pole2 cDNA ORF clone, Seriola dumerili(greater amberjack)

Gene
Clones
gRNAs

Summary

Gene Symbol	pole2
Entrez Gene ID	111225299
Full Name	DNA polymerase epsilon 2, accessory subunit
Gene Type	protein-coding
Organism	Seriola dumerili(greater amberjack)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022750074.1	XP_022605795.1	DNA polymerase epsilon subunit 2

Pathways from BioSystems
Gene Ontology

The following pole2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pole2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe219284	XM_022750074.1 Latest version!	Seriola dumerili DNA polymerase epsilon 2, accessory subunit (pole2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pole2 ( XM_022750074.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022605795.1 Seriola dumerili DNA polymerase epsilon 2, accessory subunit (pole2), mRNA.

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CloneID	OSe219284
Clone ID Related Accession (Same CDS sequence)	XM_022750074.1
Accession Version	XM_022750074.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	DNA polymerase epsilon subunit 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174404.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGTTG CTCGCAAAAT AAAGACAAAG GTGTTGGCTG GCTTCAAGAT GCGGGGACTA 
ATACTGCGAC CCGAAGCCAG CAGATACCTT GTAGAGGTGC TGGAGTCCGT AAGTCCCTCT 
GAACTGGATG ATGTTATTGA AAGGGTCCTG GATGCGGTTG AAAAGCAGCC ATTGTCCTCT 
AGTATGATAG AGCTGTCTGT GGTGGAAACC GCTGTGCAGG ACTGCACTCA GTCTTGTGAT 
GAAACCATAG ATAATGTTTT CAACATCATT GGAGCCTTCG ATGTGCCCAG ATACATTTAC 
AGCGTGGAGC GGAAGAAATT TTTACCCATC AGTATGACCA GACATCCAGC CCCGAGTCTG 
TGTGGGTTGG CCAAAGACAA AGCTGAACTC TTCAGGGAGC GCTACACAAT CTTACAACAG 
CGTACACATC GGCACGAGCT CTTCACCCCA CCTGCTATTG GAGCTGCTGT TGAAGAGGGT 
CAAAACAAAT TTCAGCTAAA GACAATTGAA GCATTGCTTG GTAGCACAGC TAAACTGGGA 
GAGGTGATTG TACTCGGGAT GATCACACAA CTGAAAGAGG GTAAATTCTA CCTGGAGGAC 
CCAAGTGGAA CAGTACAACT GGACATGTCC AAAGCACAGT TTCATAATGG CCTGTACACT 
GAATCCTGCT TTGTACTGGC AGAGGGTTGG TACGAGGACT CTGTTTTCCA TGTCAATGGG 
TTTGGGTTTC CACCAACAGA GCCATCATCA GGCACAAGGG CATACTATGG CAACATGAAC 
TTCTTTGGTG GTCCATCCAC CACTTCGGTG AAGGCCTCTG CCAAGCTGAA GCAGCTGGAG 
GAAGAGAATG AGGACGCCAT GTTTGTAATT GTTTCTGATG TGTGGCTGGA CAACGTTGAA 
GTGATGGAAA AGCTCAACAT CATGTTTTCA GGCTATGCTG CGATGCCTCC CACCTGTTTC 
ATTTTGTGTG GAAACTTTTC CTCTGCCCCA TATGGAAAGA CACAGATCAA ATCGCTCAAA 
GAGTCACTGA AGGCCCTTGC TGATGCCATA TGTGCATACC CTAACATTCA CAGCAGTAGT 
CGCTTTGTGT TTGTCCCTGG TCCTGAAGAC CCTGGTCCAG GCACCATCCT GCCAAGGCCT 
CCCCTGGCAG ACCACATCAC AGAGGAGTTC AGACAGAAGG TGCCATTTTC AGTGTTCACG 
ACTAACCCAT GCAGGATCCA GTACTGCAGC CAGGAGATCG TCATTATCAG GGAAGACCTG 
GTCAACAAGA TGTGCAGGAA CTGCGTGCGG TTGCCCAATA ACAATCTTGA CATTCCCAAC 
CATTTTGTTA AGACCATCCT GTCTCAGGGT CACCTGACTC CACTGCCTCC GTACGTCAGT 
CCTGTATTCT GGGCCTACGA CTACTCCCTG TATGTCTACC CAGTCCCTGA TGTCATCATC 
TTTGCAGACA AATACGACCC CTTCAGCATC ACCAACACGG ACTGCCTCTG TGTCAACCCA 
GGCTCCTTCC CAAGAAGTGG CTTCACATTT AAGGTGTATT ACCCATCCAA CAGGACTGTT 
GAGGACAGCA AACTTCAAGG GCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022605795.1
CDS	25..1611
Translation	MDVARKIKTKVLAGFKMRGLILRPEASRYLVEVLESVSPSELDDVIERVLDAVEKQPLSSSMIELSVVETAVQDCTQSCDETIDNVFNIIGAFDVPRYIYSVERKKFLPISMTRHPAPSLCGLAKDKAELFRERYTILQQRTHRHELFTPPAIGAAVEEGQNKFQLKTIEALLGSTAKLGEVIVLGMITQLKEGKFYLEDPSGTVQLDMSKAQFHNGLYTESCFVLAEGWYEDSVFHVNGFGFPPTEPSSGTRAYYGNMNFFGGPSTTSVKASAKLKQLEEENEDAMFVIVSDVWLDNVEVMEKLNIMFSGYAAMPPTCFILCGNFSSAPYGKTQIKSLKESLKALADAICAYPNIHSSSRFVFVPGPEDPGPGTILPRPPLADHITEEFRQKVPFSVFTTNPCRIQYCSQEIVIIREDLVNKMCRNCVRLPNNNLDIPNHFVKTILSQGHLTPLPPYVSPVFWAYDYSLYVYPVPDVIIFADKYDPFSITNTDCLCVNPGSFPRSGFTFKVYYPSNRTVEDSKLQGL

Target ORF information:

RefSeq Version	XM_022750074.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili DNA polymerase epsilon 2, accessory subunit (pole2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022750074.1

ORF Insert Sequence:

ATGGATGTTG CTCGCAAAAT AAAGACAAAG GTGTTGGCTG GCTTCAAGAT GCGGGGACTA 
ATACTGCGAC CCGAAGCCAG CAGATACCTT GTAGAGGTGC TGGAGTCCGT AAGTCCCTCT 
GAACTGGATG ATGTTATTGA AAGGGTCCTG GATGCGGTTG AAAAGCAGCC ATTGTCCTCT 
AGTATGATAG AGCTGTCTGT GGTGGAAACC GCTGTGCAGG ACTGCACTCA GTCTTGTGAT 
GAAACCATAG ATAATGTTTT CAACATCATT GGAGCCTTCG ATGTGCCCAG ATACATTTAC 
AGCGTGGAGC GGAAGAAATT TTTACCCATC AGTATGACCA GACATCCAGC CCCGAGTCTG 
TGTGGGTTGG CCAAAGACAA AGCTGAACTC TTCAGGGAGC GCTACACAAT CTTACAACAG 
CGTACACATC GGCACGAGCT CTTCACCCCA CCTGCTATTG GAGCTGCTGT TGAAGAGGGT 
CAAAACAAAT TTCAGCTAAA GACAATTGAA GCATTGCTTG GTAGCACAGC TAAACTGGGA 
GAGGTGATTG TACTCGGGAT GATCACACAA CTGAAAGAGG GTAAATTCTA CCTGGAGGAC 
CCAAGTGGAA CAGTACAACT GGACATGTCC AAAGCACAGT TTCATAATGG CCTGTACACT 
GAATCCTGCT TTGTACTGGC AGAGGGTTGG TACGAGGACT CTGTTTTCCA TGTCAATGGG 
TTTGGGTTTC CACCAACAGA GCCATCATCA GGCACAAGGG CATACTATGG CAACATGAAC 
TTCTTTGGTG GTCCATCCAC CACTTCGGTG AAGGCCTCTG CCAAGCTGAA GCAGCTGGAG 
GAAGAGAATG AGGACGCCAT GTTTGTAATT GTTTCTGATG TGTGGCTGGA CAACGTTGAA 
GTGATGGAAA AGCTCAACAT CATGTTTTCA GGCTATGCTG CGATGCCTCC CACCTGTTTC 
ATTTTGTGTG GAAACTTTTC CTCTGCCCCA TATGGAAAGA CACAGATCAA ATCGCTCAAA 
GAGTCACTGA AGGCCCTTGC TGATGCCATA TGTGCATACC CTAACATTCA CAGCAGTAGT 
CGCTTTGTGT TTGTCCCTGG TCCTGAAGAC CCTGGTCCAG GCACCATCCT GCCAAGGCCT 
CCCCTGGCAG ACCACATCAC AGAGGAGTTC AGACAGAAGG TGCCATTTTC AGTGTTCACG 
ACTAACCCAT GCAGGATCCA GTACTGCAGC CAGGAGATCG TCATTATCAG GGAAGACCTG 
GTCAACAAGA TGTGCAGGAA CTGCGTGCGG TTGCCCAATA ACAATCTTGA CATTCCCAAC 
CATTTTGTTA AGACCATCCT GTCTCAGGGT CACCTGACTC CACTGCCTCC GTACGTCAGT 
CCTGTATTCT GGGCCTACGA CTACTCCCTG TATGTCTACC CAGTCCCTGA TGTCATCATC 
TTTGCAGACA AATACGACCC CTTCAGCATC ACCAACACGG ACTGCCTCTG TGTCAACCCA 
GGCTCCTTCC CAAGAAGTGG CTTCACATTT AAGGTGTATT ACCCATCCAA CAGGACTGTT 
GAGGACAGCA AACTTCAAGG GCTCTAA