mest cDNA ORF clone, Seriola dumerili(greater amberjack)

Gene
Clones
gRNAs

Summary

Gene Symbol	mest
Entrez Gene ID	111224912
Full Name	mesoderm specific transcript
Gene Type	protein-coding
Organism	Seriola dumerili(greater amberjack)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022749609.1	XP_022605330.1	mesoderm-specific transcript homolog protein

Pathways from BioSystems
Gene Ontology

The following mest gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mest cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe218918	XM_022749609.1 Latest version!	Seriola dumerili mesoderm specific transcript (mest), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mest ( XM_022749609.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022605330.1 Seriola dumerili mesoderm specific transcript (mest), mRNA.

Price & Availability↑

CloneID	OSe218918
Clone ID Related Accession (Same CDS sequence)	XM_022749609.1
Accession Version	XM_022749609.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	mesoderm-specific transcript homolog protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174393.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAGT GGTGGATTCA TGTGGGTTTG ATCTGCATCC CGCTGGTGGC CGTCTACCTG 
CACATCCCGC CCCCTCAGCT CTCACCTGCC CTACAGAAGT GGCACAATGC CGGAGAGGTG 
TTCCACTTCA GAGGCAGGAA GATCTTCTAC AGAGACTCCT ATGGTGCTCT TGGAAGCTCT 
GACATTGTCA TTCTGCTCCA CGGCTTCCCC ACCTCCAGCT ACGACTGGAA CAAGATCTGG 
GAGCCGCTCA CACAGCGCTT CCATCGAGTC ATTGCCCTGG ACTTCCTGGG GTTTGGCTTT 
AGCGACAAGC CACGACCACA CAGGTACTCT ATCTTCGAGC AGGCCAGTGT GGTGGAGGCG 
CTGGTATCCA ATTTGGGTCT GAGCAACCAG CGAGTCAACC TGGTGTCCCA TGACTACGGT 
GACACTGTGG CCCTGGAGCT GCTCTACAGG AGTGACCAAA ATCGCACAGG TCACCTGACC 
TTTAACAGCC TGTGTCTTTC AAACGGAGGC ATATTCCCAG AAACACACTA CCCACGGCTG 
CTGCAGACGC TTCTGAAGGA CTCCAGTTTT CTGGCTCCAC TCCTGACTCG TCTCACCAAC 
TATATGATCT TCCAAAAGGG GATCGGGGAA GTGTTCGGTC CGTACACGCA GCCCACAGAC 
GCTGAGTTCT GGGACATGTG GACGGGTTTG CGCTACAATG ATGGCAACCT AGTTTTGGAC 
AGTATTCTCC AGTACATCAA CCAGAGATTG AAACACAGAG AGCGGTGGGT GGGTGCGCTC 
ACTTCCACCT TTGTCCCACT GCACATGATC TACGGACCCC TGGACCCGGT CAACCCCCAT 
CCTCAGTTCA TCCGTGTTTA CCAGCAGCTG GTCCAGAGGT CGACAGTCAC CATTTTGGAT 
GAACACATCA GTCACTACCC TCAGCTGGAG GATCCCACTG GCTTCCTCAA TGCATATTTC 
AATTTTATTC ACTCTTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022605330.1
CDS	278..1258
Translation	MREWWIHVGLICIPLVAVYLHIPPPQLSPALQKWHNAGEVFHFRGRKIFYRDSYGALGSSDIVILLHGFPTSSYDWNKIWEPLTQRFHRVIALDFLGFGFSDKPRPHRYSIFEQASVVEALVSNLGLSNQRVNLVSHDYGDTVALELLYRSDQNRTGHLTFNSLCLSNGGIFPETHYPRLLQTLLKDSSFLAPLLTRLTNYMIFQKGIGEVFGPYTQPTDAEFWDMWTGLRYNDGNLVLDSILQYINQRLKHRERWVGALTSTFVPLHMIYGPLDPVNPHPQFIRVYQQLVQRSTVTILDEHISHYPQLEDPTGFLNAYFNFIHSF

Target ORF information:

RefSeq Version	XM_022749609.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili mesoderm specific transcript (mest), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022749609.1

ORF Insert Sequence:

ATGAGGGAGT GGTGGATTCA TGTGGGTTTG ATCTGCATCC CGCTGGTGGC CGTCTACCTG 
CACATCCCGC CCCCTCAGCT CTCACCTGCC CTACAGAAGT GGCACAATGC CGGAGAGGTG 
TTCCACTTCA GAGGCAGGAA GATCTTCTAC AGAGACTCCT ATGGTGCTCT TGGAAGCTCT 
GACATTGTCA TTCTGCTCCA CGGCTTCCCC ACCTCCAGCT ACGACTGGAA CAAGATCTGG 
GAGCCGCTCA CACAGCGCTT CCATCGAGTC ATTGCCCTGG ACTTCCTGGG GTTTGGCTTT 
AGCGACAAGC CACGACCACA CAGGTACTCT ATCTTCGAGC AGGCCAGTGT GGTGGAGGCG 
CTGGTATCCA ATTTGGGTCT GAGCAACCAG CGAGTCAACC TGGTGTCCCA TGACTACGGT 
GACACTGTGG CCCTGGAGCT GCTCTACAGG AGTGACCAAA ATCGCACAGG TCACCTGACC 
TTTAACAGCC TGTGTCTTTC AAACGGAGGC ATATTCCCAG AAACACACTA CCCACGGCTG 
CTGCAGACGC TTCTGAAGGA CTCCAGTTTT CTGGCTCCAC TCCTGACTCG TCTCACCAAC 
TATATGATCT TCCAAAAGGG GATCGGGGAA GTGTTCGGTC CGTACACGCA GCCCACAGAC 
GCTGAGTTCT GGGACATGTG GACGGGTTTG CGCTACAATG ATGGCAACCT AGTTTTGGAC 
AGTATTCTCC AGTACATCAA CCAGAGATTG AAACACAGAG AGCGGTGGGT GGGTGCGCTC 
ACTTCCACCT TTGTCCCACT GCACATGATC TACGGACCCC TGGACCCGGT CAACCCCCAT 
CCTCAGTTCA TCCGTGTTTA CCAGCAGCTG GTCCAGAGGT CGACAGTCAC CATTTTGGAT 
GAACACATCA GTCACTACCC TCAGCTGGAG GATCCCACTG GCTTCCTCAA TGCATATTTC 
AATTTTATTC ACTCTTTCTG A