fbln1 cDNA ORF clone, Seriola dumerili(greater amberjack)

Gene
Clones
gRNAs

Summary

Gene Symbol	fbln1
Entrez Gene ID	111224801
Full Name	fibulin 1
Gene Type	protein-coding
Organism	Seriola dumerili(greater amberjack)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022749451.1	XP_022605172.1	fibulin-1

Pathways from BioSystems
Gene Ontology

The following fbln1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fbln1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe218794	XM_022749451.1 Latest version!	Seriola dumerili fibulin 1 (fbln1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fbln1 ( XM_022749451.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022605172.1 Seriola dumerili fibulin 1 (fbln1), mRNA.

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CloneID	OSe218794
Clone ID Related Accession (Same CDS sequence)	XM_022749451.1
Accession Version	XM_022749451.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	fibulin-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174393.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAGT GGACTATACT TTTGTTCTCT TTGTACGGAG TTCTGCATGG AGAAGAGATT 
CAGCCTCCTA CTGTAGAAAA GTGCTGTCAG GATGGGAGGG ATCGCGCCCT GGGAGGACAT 
GACTGTACAA TTCTCCCCCT CATCTCGTCC TCCCACAACT GCAGGATCGC CCAGGAGCAG 
TGCTGTGCAG CAGCAGTACT AGACAAGCTC TGTGACAATG GAATCAAACT GGCCCACAGT 
CAAGGGGCCT GTGAGAGGCC CTTCTTCCAA GGAGACCCCT GGGAAACTGA AATCTCGAAG 
ATGTGTTGTG ACTGCTGTAT GCTCGGCCTG ATGGCAGTGG GCCGGGGTTC GAGCTGCGAG 
CTGCAGGGCC TGTTGCTGGG GAGACAGTGT GCACACACAG CTGAAACCTG CTGTGGCAAA 
AACACAACAG CGGACGTCAA ACGAAATGTT GAGGTTCCAG CGACAGCCGA GCCTAAAGCC 
AGCATTACTA CCAAGCCTCC AGAGACACTG GACTCCTGTA AAGACTCCAA TTGCTCCCAG 
CTGTGTGTAG GTAATGGATC ATGTACCTGC CACGATGGTT ATCAACTGCA AAAGGATGGA 
GTGAATTGTG AGGATGTTAA TGAGTGTGTG ACGGGAGGCC ACAACTGCAT CTTCGGCCAG 
TTTTGCATCA ACACAGAGGG CTCCTTTCGC TGTCAGAGGG AAACCAGCTG TGGTACAGGG 
TATGATCTCA AAGAAAACAA CAGTTGCCAA GACATTGATG AGTGTGCGTT GGGGATCCAT 
AACTGTGGAC CAGACTTTTC ATGTACAAAC ACAGCCGGCT CGTTCCGCTG CCACCCAAAG 
GAGAGGTGCG CTGACGGTTA CATACAGGAC GCCGCCGGCA GCTGTATCGA TATAAATGAG 
TGTGTGGCCC GCAGCAGCCC ATGCCTGCCT GGCCACACTT GTATCAACAC AATAGGCTCG 
TACACCTGTC GCAGGAACAC AGTCACCTGT GGACGAGGCT ATCACCTAAC CGAGGACGGC 
ACGCGCTGTG AAGATGTAGA TGAGTGTCGC ACAGGTAATG TGTGTGGTGA GCACGGCTGT 
GTGAACTTCG TGGGATCGTA CCGCTGTGAG TGCAGAATCG GCTTCATCTT CAACAGCATC 
ACCAAACTGT GCGAAGATAT CAATGAGTGC AGGCATTATC CCGGACGGCT CTGCGCCCAC 
AAGTGTGAGA ATACAGAGGG GTCCTACCAG TGCAGCTGCA CCACTGGCTT CAAACTGTCC 
CATGATGGCA GAAACTGTGA TGATGTGAAC GAGTGTGAGG CCAACCCTTG CAGCCAGGAA 
TGTGCCAATG TCTTTGGCTC CTATCAGTGC TACTGTCGCC GTGGTTACCA GCTGAGTGAC 
ATTGACGGGA TAACATGCGA AGATATTGAT GAGTGTGCTC TGCCTGCTGG AGGTCATGTG 
TGTTCCTATC GCTGCTCCAA CGCCCCAGGA AGTTTCTACT GCACCTGCCC TCTCACTGGC 
TACACACTCA CCCCCAATGG ACGCACATGT CAAGACATTG ATGAATGTGC AGCAGGGAGC 
CACACCTGTT CTGTCTCTGA GAGCTGCGTC AACGTCCAGG GAGGGTTTCG CTGCCTGTCC 
TTCCAGTGTC CTCCAAACTT CCGACAAGCA GCACAGGGAT CCAGACCTGA TGTCTCAGCT 
GGTTTGCGTT GTATTAAGGC CTGCCAACCT AACAACATCG GCTGTGCTCT GGACCCCGTC 
CACCTCATCA CCCACACCGT CCTCTCTCTG CCAACCTTCA GAGACTTCGA TGGACCAGAG 
GAAATAGTTT TCCTGCGGAC AACTGTGGCG GCTAACCCTG CTCTTCTCCC TGGCGCAACC 
GATGTTCTCT TTGAAATCGT GGCTGCAGAT GAGCAGTTCT CCTTTGACGT GGTGAAGCGC 
TCCCACCAGG GCTTGATTAT GGGTGTTGTT CAGCAGGTGA AACCTATCAT TGGCCCCAGG 
GATCTTGTGT TGGAGGTGTC TATGAATTAT GTCAAGTCAG GGGTCATTTC CCATCGCAAC 
GTTGTCATGG TCCACGTCTT TATCTCTGAG TTCTGGTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022605172.1
CDS	115..2196
Translation	MAQWTILLFSLYGVLHGEEIQPPTVEKCCQDGRDRALGGHDCTILPLISSSHNCRIAQEQCCAAAVLDKLCDNGIKLAHSQGACERPFFQGDPWETEISKMCCDCCMLGLMAVGRGSSCELQGLLLGRQCAHTAETCCGKNTTADVKRNVEVPATAEPKASITTKPPETLDSCKDSNCSQLCVGNGSCTCHDGYQLQKDGVNCEDVNECVTGGHNCIFGQFCINTEGSFRCQRETSCGTGYDLKENNSCQDIDECALGIHNCGPDFSCTNTAGSFRCHPKERCADGYIQDAAGSCIDINECVARSSPCLPGHTCINTIGSYTCRRNTVTCGRGYHLTEDGTRCEDVDECRTGNVCGEHGCVNFVGSYRCECRIGFIFNSITKLCEDINECRHYPGRLCAHKCENTEGSYQCSCTTGFKLSHDGRNCDDVNECEANPCSQECANVFGSYQCYCRRGYQLSDIDGITCEDIDECALPAGGHVCSYRCSNAPGSFYCTCPLTGYTLTPNGRTCQDIDECAAGSHTCSVSESCVNVQGGFRCLSFQCPPNFRQAAQGSRPDVSAGLRCIKACQPNNIGCALDPVHLITHTVLSLPTFRDFDGPEEIVFLRTTVAANPALLPGATDVLFEIVAADEQFSFDVVKRSHQGLIMGVVQQVKPIIGPRDLVLEVSMNYVKSGVISHRNVVMVHVFISEFWF

Target ORF information:

RefSeq Version	XM_022749451.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili fibulin 1 (fbln1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022749451.1

ORF Insert Sequence:

ATGGCGCAGT GGACTATACT TTTGTTCTCT TTGTACGGAG TTCTGCATGG AGAAGAGATT 
CAGCCTCCTA CTGTAGAAAA GTGCTGTCAG GATGGGAGGG ATCGCGCCCT GGGAGGACAT 
GACTGTACAA TTCTCCCCCT CATCTCGTCC TCCCACAACT GCAGGATCGC CCAGGAGCAG 
TGCTGTGCAG CAGCAGTACT AGACAAGCTC TGTGACAATG GAATCAAACT GGCCCACAGT 
CAAGGGGCCT GTGAGAGGCC CTTCTTCCAA GGAGACCCCT GGGAAACTGA AATCTCGAAG 
ATGTGTTGTG ACTGCTGTAT GCTCGGCCTG ATGGCAGTGG GCCGGGGTTC GAGCTGCGAG 
CTGCAGGGCC TGTTGCTGGG GAGACAGTGT GCACACACAG CTGAAACCTG CTGTGGCAAA 
AACACAACAG CGGACGTCAA ACGAAATGTT GAGGTTCCAG CGACAGCCGA GCCTAAAGCC 
AGCATTACTA CCAAGCCTCC AGAGACACTG GACTCCTGTA AAGACTCCAA TTGCTCCCAG 
CTGTGTGTAG GTAATGGATC ATGTACCTGC CACGATGGTT ATCAACTGCA AAAGGATGGA 
GTGAATTGTG AGGATGTTAA TGAGTGTGTG ACGGGAGGCC ACAACTGCAT CTTCGGCCAG 
TTTTGCATCA ACACAGAGGG CTCCTTTCGC TGTCAGAGGG AAACCAGCTG TGGTACAGGG 
TATGATCTCA AAGAAAACAA CAGTTGCCAA GACATTGATG AGTGTGCGTT GGGGATCCAT 
AACTGTGGAC CAGACTTTTC ATGTACAAAC ACAGCCGGCT CGTTCCGCTG CCACCCAAAG 
GAGAGGTGCG CTGACGGTTA CATACAGGAC GCCGCCGGCA GCTGTATCGA TATAAATGAG 
TGTGTGGCCC GCAGCAGCCC ATGCCTGCCT GGCCACACTT GTATCAACAC AATAGGCTCG 
TACACCTGTC GCAGGAACAC AGTCACCTGT GGACGAGGCT ATCACCTAAC CGAGGACGGC 
ACGCGCTGTG AAGATGTAGA TGAGTGTCGC ACAGGTAATG TGTGTGGTGA GCACGGCTGT 
GTGAACTTCG TGGGATCGTA CCGCTGTGAG TGCAGAATCG GCTTCATCTT CAACAGCATC 
ACCAAACTGT GCGAAGATAT CAATGAGTGC AGGCATTATC CCGGACGGCT CTGCGCCCAC 
AAGTGTGAGA ATACAGAGGG GTCCTACCAG TGCAGCTGCA CCACTGGCTT CAAACTGTCC 
CATGATGGCA GAAACTGTGA TGATGTGAAC GAGTGTGAGG CCAACCCTTG CAGCCAGGAA 
TGTGCCAATG TCTTTGGCTC CTATCAGTGC TACTGTCGCC GTGGTTACCA GCTGAGTGAC 
ATTGACGGGA TAACATGCGA AGATATTGAT GAGTGTGCTC TGCCTGCTGG AGGTCATGTG 
TGTTCCTATC GCTGCTCCAA CGCCCCAGGA AGTTTCTACT GCACCTGCCC TCTCACTGGC 
TACACACTCA CCCCCAATGG ACGCACATGT CAAGACATTG ATGAATGTGC AGCAGGGAGC 
CACACCTGTT CTGTCTCTGA GAGCTGCGTC AACGTCCAGG GAGGGTTTCG CTGCCTGTCC 
TTCCAGTGTC CTCCAAACTT CCGACAAGCA GCACAGGGAT CCAGACCTGA TGTCTCAGCT 
GGTTTGCGTT GTATTAAGGC CTGCCAACCT AACAACATCG GCTGTGCTCT GGACCCCGTC 
CACCTCATCA CCCACACCGT CCTCTCTCTG CCAACCTTCA GAGACTTCGA TGGACCAGAG 
GAAATAGTTT TCCTGCGGAC AACTGTGGCG GCTAACCCTG CTCTTCTCCC TGGCGCAACC 
GATGTTCTCT TTGAAATCGT GGCTGCAGAT GAGCAGTTCT CCTTTGACGT GGTGAAGCGC 
TCCCACCAGG GCTTGATTAT GGGTGTTGTT CAGCAGGTGA AACCTATCAT TGGCCCCAGG 
GATCTTGTGT TGGAGGTGTC TATGAATTAT GTCAAGTCAG GGGTCATTTC CCATCGCAAC 
GTTGTCATGG TCCACGTCTT TATCTCTGAG TTCTGGTTCT GA