plk1 cDNA ORF clone, Seriola dumerili(greater amberjack)

Gene
Clones
gRNAs

Summary

Gene Symbol	plk1
Entrez Gene ID	111216631
Full Name	polo like kinase 1
Gene Type	protein-coding
Organism	Seriola dumerili(greater amberjack)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022738219.1	XP_022593940.1	serine/threonine-protein kinase PLK1

Pathways from BioSystems
Gene Ontology

The following plk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the plk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe209664	XM_022738219.1 Latest version!	Seriola dumerili polo like kinase 1 (plk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

plk1 ( XM_022738219.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022593940.1 Seriola dumerili polo like kinase 1 (plk1), mRNA.

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CloneID	OSe209664
Clone ID Related Accession (Same CDS sequence)	XM_022738219.1
Accession Version	XM_022738219.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	serine/threonine-protein kinase PLK1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174290.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGCAG GTATTCCGAA GCCGGCGAAT CCGTCGGCCC ATGTCGACCC CAAATCAGCT 
CCTCTCAAAG AAATCCCAGA TGTTCTGGTG GACCCACGCA CCATGAGGAG ATACACGAGG 
GGAAGATTCC TTGGAAAAGG TGGTTTCGCC AAATGCTATG AAATCACAGA TATGGAGACG 
AAGCAGGTTT TCGCCGGAAA AATCGTGCCC AAGTCCCTCA TCCTGAAGCA GCACCAGAGG 
GAGAAGATGT CCTCGGAAAT CGCCATTCAC AAGAGCCTCG ACCATGCGAA CATTGTGGGG 
TTCCACGGGT TTTTCGAAGA CGACGACTTT GTCTTTGTTG TCCTGGAAAT CTGCAGGAGA 
AGGTCCTTGT TGGAGCTGCA CAAGCGTCGT AAGGCTGTGA CTGAGCCAGA GGCTCGCTAC 
TACATGACAC AGCTGCTCAA GGGTGTCCAG TACCTGCACA ACAACCGAGT CATCCACAGA 
GACCTGAAAC TGGGCAACAT CTTCCTCAAT GATGACATGG AGGTCAAGAT AGGGGACTTT 
GGCCTGGCCA CTAAGATCGA GTTTGATGGC GAGAGGAAGA AGACCTTGTG TGGAACACCC 
AACTACATTG CACCAGAAGT GCTTTGCAAG AAAGGCCACA GCTATGAAGT GGACGTCTGG 
TCGCTTGGGT GTATATTGTA CACTTTGTTG GTGGGTAAGC CCCCATTCGA GACCTCCTGT 
CTGAAGGAGA CCTACAACCG CATCAAGAAA AACAACTACA CTGTCCCCTG GCACATCAAC 
CCGTCCGCAT CTGCTCTCAT CAAGCGGATG CTGCACGCTG ATCCTGCTCA GAGGCCCACC 
ATCGCTGAGC TGCAAACAGA TGAGTTCTTC ACATCCGGCT ACATCCCCTT ACGTCTGCCC 
ACTACATGCC TCACTGTGCC CCCGCGGTTC TCCATCGCTC CCTCCACAGC TATGGAGCTC 
AGCCAGAGGC GCCCCCTGAC TGCTATTAAC AACAAGGCTG GAACAGAGAA GGTGGACGTT 
AAGGATGAAC CTGTTCAAAG GGAGACTGAG GCGTCAGAAT GCCACCTGAA AGACATGCTA 
CAGCAGCTCA ACAGTATCAT TGCTGCCAAG CCCTCTGAGA AGGCACTCAT ACGCCAGGAA 
GAGGCAGAGG ATCCTGCATG TATCCCCATC TTCTGGATCA GCAAATGGGT CGACTACTCT 
GACAAATATG GACTAGGCTA CCAGCTCTGT GACAACAGCG TGGGCGTGCT GTTCAACGAT 
TACACTCGCC TGATCATGTA CACCGACGGA GACAGTCTGC AGTACATCGA CAAGACGGCG 
GCTGAATCCT ACCTCAGCGT GCGCTCTTGC CCTGCTACCC TCAACAAGAA GATAACACTG 
CTGAAATATT TCCGCAATTA CATGAGTGAG CACCTGTTGA AGGCCGGTGC GAACATGGCA 
CGGCGGGAAG GAGATGAGCT TGCCAGGCTG CCTTACCTCT CCCTCTGGTT CAGAACAAAG 
AGCGCCATCG TCCTGCACCT CACCAACGGC ACCGTTCAGA TCAACTTCTT CCAGGACCAC 
ACCAAGCTGA TCCTGTGTCC GCTGATGGCC GCGGTGACCT ACATCGACGA GAAGCGAGAA 
TTCCGCACCT ACAAGCTGTC TCTGCTGGAG GAGTTCGGTT GCAGTAAGGA GCTGTCCAGC 
CGCATCTGCT ACGCCAAGCT CATGGTGCAG AAACTGCTGG ACAGCAAACT CACCCCCGCT 
GCACACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022593940.1
CDS	304..2052
Translation	MSAGIPKPANPSAHVDPKSAPLKEIPDVLVDPRTMRRYTRGRFLGKGGFAKCYEITDMETKQVFAGKIVPKSLILKQHQREKMSSEIAIHKSLDHANIVGFHGFFEDDDFVFVVLEICRRRSLLELHKRRKAVTEPEARYYMTQLLKGVQYLHNNRVIHRDLKLGNIFLNDDMEVKIGDFGLATKIEFDGERKKTLCGTPNYIAPEVLCKKGHSYEVDVWSLGCILYTLLVGKPPFETSCLKETYNRIKKNNYTVPWHINPSASALIKRMLHADPAQRPTIAELQTDEFFTSGYIPLRLPTTCLTVPPRFSIAPSTAMELSQRRPLTAINNKAGTEKVDVKDEPVQRETEASECHLKDMLQQLNSIIAAKPSEKALIRQEEAEDPACIPIFWISKWVDYSDKYGLGYQLCDNSVGVLFNDYTRLIMYTDGDSLQYIDKTAAESYLSVRSCPATLNKKITLLKYFRNYMSEHLLKAGANMARREGDELARLPYLSLWFRTKSAIVLHLTNGTVQINFFQDHTKLILCPLMAAVTYIDEKREFRTYKLSLLEEFGCSKELSSRICYAKLMVQKLLDSKLTPAAH

Target ORF information:

RefSeq Version	XM_022738219.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili polo like kinase 1 (plk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022738219.1

ORF Insert Sequence:

ATGAGTGCAG GTATTCCGAA GCCGGCGAAT CCGTCGGCCC ATGTCGACCC CAAATCAGCT 
CCTCTCAAAG AAATCCCAGA TGTTCTGGTG GACCCACGCA CCATGAGGAG ATACACGAGG 
GGAAGATTCC TTGGAAAAGG TGGTTTCGCC AAATGCTATG AAATCACAGA TATGGAGACG 
AAGCAGGTTT TCGCCGGAAA AATCGTGCCC AAGTCCCTCA TCCTGAAGCA GCACCAGAGG 
GAGAAGATGT CCTCGGAAAT CGCCATTCAC AAGAGCCTCG ACCATGCGAA CATTGTGGGG 
TTCCACGGGT TTTTCGAAGA CGACGACTTT GTCTTTGTTG TCCTGGAAAT CTGCAGGAGA 
AGGTCCTTGT TGGAGCTGCA CAAGCGTCGT AAGGCTGTGA CTGAGCCAGA GGCTCGCTAC 
TACATGACAC AGCTGCTCAA GGGTGTCCAG TACCTGCACA ACAACCGAGT CATCCACAGA 
GACCTGAAAC TGGGCAACAT CTTCCTCAAT GATGACATGG AGGTCAAGAT AGGGGACTTT 
GGCCTGGCCA CTAAGATCGA GTTTGATGGC GAGAGGAAGA AGACCTTGTG TGGAACACCC 
AACTACATTG CACCAGAAGT GCTTTGCAAG AAAGGCCACA GCTATGAAGT GGACGTCTGG 
TCGCTTGGGT GTATATTGTA CACTTTGTTG GTGGGTAAGC CCCCATTCGA GACCTCCTGT 
CTGAAGGAGA CCTACAACCG CATCAAGAAA AACAACTACA CTGTCCCCTG GCACATCAAC 
CCGTCCGCAT CTGCTCTCAT CAAGCGGATG CTGCACGCTG ATCCTGCTCA GAGGCCCACC 
ATCGCTGAGC TGCAAACAGA TGAGTTCTTC ACATCCGGCT ACATCCCCTT ACGTCTGCCC 
ACTACATGCC TCACTGTGCC CCCGCGGTTC TCCATCGCTC CCTCCACAGC TATGGAGCTC 
AGCCAGAGGC GCCCCCTGAC TGCTATTAAC AACAAGGCTG GAACAGAGAA GGTGGACGTT 
AAGGATGAAC CTGTTCAAAG GGAGACTGAG GCGTCAGAAT GCCACCTGAA AGACATGCTA 
CAGCAGCTCA ACAGTATCAT TGCTGCCAAG CCCTCTGAGA AGGCACTCAT ACGCCAGGAA 
GAGGCAGAGG ATCCTGCATG TATCCCCATC TTCTGGATCA GCAAATGGGT CGACTACTCT 
GACAAATATG GACTAGGCTA CCAGCTCTGT GACAACAGCG TGGGCGTGCT GTTCAACGAT 
TACACTCGCC TGATCATGTA CACCGACGGA GACAGTCTGC AGTACATCGA CAAGACGGCG 
GCTGAATCCT ACCTCAGCGT GCGCTCTTGC CCTGCTACCC TCAACAAGAA GATAACACTG 
CTGAAATATT TCCGCAATTA CATGAGTGAG CACCTGTTGA AGGCCGGTGC GAACATGGCA 
CGGCGGGAAG GAGATGAGCT TGCCAGGCTG CCTTACCTCT CCCTCTGGTT CAGAACAAAG 
AGCGCCATCG TCCTGCACCT CACCAACGGC ACCGTTCAGA TCAACTTCTT CCAGGACCAC 
ACCAAGCTGA TCCTGTGTCC GCTGATGGCC GCGGTGACCT ACATCGACGA GAAGCGAGAA 
TTCCGCACCT ACAAGCTGTC TCTGCTGGAG GAGTTCGGTT GCAGTAAGGA GCTGTCCAGC 
CGCATCTGCT ACGCCAAGCT CATGGTGCAG AAACTGCTGG ACAGCAAACT CACCCCCGCT 
GCACACTAG