crim1 cDNA ORF clone, Scleropages formosus(Asian bonytongue)

Gene
Clones
gRNAs

Summary

Gene Symbol	crim1
Entrez Gene ID	108922283
Full Name	cysteine rich transmembrane BMP regulator 1
Gene Type	protein-coding
Organism	Scleropages formosus(Asian bonytongue)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018732332.1	XP_018587848.1	cysteine-rich motor neuron 1 protein
XM_018732332.2	XP_018587848.1	cysteine-rich motor neuron 1 protein

Pathways from BioSystems
Gene Ontology

The following crim1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crim1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc145092	XM_018732332.2 Latest version!	Scleropages formosus cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSc35172	XM_018732332.1 Latest version!	Scleropages formosus cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crim1 ( XM_018732332.2 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018587848.1 Scleropages formosus cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

Price & Availability↑

CloneID	OSc145092
Clone ID Related Accession (Same CDS sequence)	XM_018732332.2
Accession Version	XM_018732332.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-21
Organism	Scleropages formosus(Asian bonytongue)
Product	cysteine-rich motor neuron 1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041820.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2019 this sequence version replaced XM_018732332.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Scleropages formosus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCCT CCAGGATGTA CCTTCTCGCA CTGAAATGCA TCGTGATCTC GCAGCTCGCC 
GTGCTCATGG CCAAGGGCAG CCGCGCTCTG CTCTGCCTGC CGTGCGATGA GTCCAAGTGC 
GAGGAGCCGA GGCACTGCAG GGGCGCCGTG GTGCTCGGCG TGTGCGGCTG CTGCTCCGTG 
TGCGCCAAGC AGAGGAACGA GAGCTGCGGC GGAGTCTACG GGCTGTACGG GACGTGCGAT 
CGCGGGCTGC GCTGCGTCAT CCGACCGGCG CTCGGCGGAG TCTCCGTCAC GCAGTACGAG 
GTCGGCGTCT GCGAAGATGA GAACTGGGAT GATGACCAGC TTTTGGGCTT TGAGCCCTGC 
AATGAAAACC TCATCACGGG CTGCAACATC ATCGACGGGA AGTGCGAGTG CGACAGCCTG 
CGGACGTGCA GCAACCCTTT CGAGTTCCCC AGCCAGGGGG CCTGTCAGGC TGCGCTCAAG 
AAGATCGAAG AGGAGAAGCC GGACTGCTCC TCAGTGCGCT GCGAGGTCCA GTTCTCACCC 
CGCTGTCCTG AGGACTCCGT GCTGATCGAA GGCTACGCCC CCCCGGGTGA ATGTTGCCCG 
CTGCCCAGCC GCTGCGTTTG CAGTCCAGCC GGCTGCCTGC GCAAAGTGTG CCAGCCGGGT 
TACCTCAACA TCCTGGTGTC CAAGGGTTCG GGGAAGCCCG GCGAGTGTTG CGACCTGTAC 
GAATGCAAGC CAGTTTTCAG CGTGGACTGT AGAACGGTGG AGTGTCCGCC AGTCCAACAA 
GTTCAGTGCC CCCCTGACAG CTATGAGACC CAGGTCCGCC TCACCGCAGA TGGCTGCTGT 
ACCCTGCCAA CCAGATGTGA ATGTTTACCT GGCCTGTGTG GCTTCCCCCA GTGCTCCGCA 
GGAAGCGTTC CCCGTGTCAT TTCCCGCGGC GACGGCTCTC CGGGGAGGTG CTGCGACGTC 
TTTGAGTGCG TCAACGAGAC TAAGCCGCCA TGCTCCTTCA ATGGGGTGGA GTACCGAGAT 
GGAGACATGT TCCGGATGGA CGCCTGCCGC TTCTGCCGCT GCCAGGGCGG AGTGTCCGTC 
TGCTTCCAGG CACAATGTGG TGTCCTCCAC TGCCAGCGCT ACTACGTTCC CGAAGGAGAG 
TGCTGTCCCG TCTGTGAAGA CACCAGCTAC CCGGCTCTGA ATTATGCCGG ATGCTTTGTC 
AACGGTCAGA TCTTAGCTCA CGGCGACCAC TGGCGTGAGG ACGACTGCAC ATTCTGCCAG 
TGTGTGAGCG GGGAGGCACG GTGCGTGGCA GCTGCCTGCG GACACAGCTG TGTTAACCCC 
GTCACCGTGC CTGGGGAGTG CTGCCCTGTC TGCGAAGAGC CCACCTACAT CACCATGGCT 
CCCCCTGCTT GTGACTCCAT GAAAAACTGC AGCCTAACAG AGCAGGACTG TATCTACGGC 
TTCAAACGAG ACCAGGACAA CTGCCGCATC TGCCAGTGCA AGACACGAGA GGAGTTGTGC 
AGGGGCCTCA TGGCGGACTG CACCCTCGAC TGCCCCTTTG GTTTCCAAAC CGACGTCCAC 
GGATGCGGAG TATGTCAGTG TCGACCGAGG CCCAAAAAGT GCAAACCCGT GTCCTGCGAC 
AAGGAATGCC CCTTTGGCTT CATAAAGAAC AAGCATGGCT GTGAAGTGTG CCGCTGCAGG 
AAGTGCCCAG AGGTGCCATG CGACAAGTCC TGTCCATTTG GCTTCCAGCT TGACCGGCTG 
GGCTGCCTCA CCTGCCAGTG CAAAGACCAA AGCACTTCAT CTGCGGAACC CTCAGTGAAA 
CTGGGCTCCT GCTTGTCCAT GGATGGCCGT CGGCACGACA ACGGTGACAA CTGGCATGAC 
GGCTGTCGGG ACTGCTACTG CCACAATGGC AGGGAGATGT GCGCTCTGAT TTCGTGCCCT 
GTGCCCGTCT GCGCCAACCC TACCATTCGA CCTGGCCAGT GCTGCCCGTC TTGTGCAGAT 
GACCCCAGCT CCCACAAGCC TGAGCTCAGC GAGGCATCCG TGTGCCTGGC CCCCAGCGGG 
GAGTACTTTG TGGAGGGTGA AACTTGGAAC ATCGACTCAT GCACGCAGTG CACATGCCAC 
AGCGGCAGGG TGCTGTGTGA GACGGAGGTT TGCCCACCCC TGCTATGCCA CAGTCCCATC 
AGGACACAGG ACTCCTGCTG TCCACATTGC CCAGATGAGC CCCTCACTCC ACAGGCCCCC 
AGCAATGACA GCATGCCCAG CTACTGCCGC AATGAGGAGG GTGACATCTT CCTGGCAGCT 
GAGTCCTGGA AGCCCAACAT GTGCTCCAGT TGCGTCTGCC TGGACGGCGT CATCAGCTGC 
TTCTCCGAGT CCTGCCCGGT GGTGGGCTGC GCGAGGCCTG TGCTGCGCAA GGGCCAGTGC 
TGCCCGTACT GCCTAGAGGA CACGGTGCCA AGGGCGGTGT GCCACTTCAA TGGGAAGACA 
TACGCCGACG AGGAGCGCTG GGACATCGAC AGCTGCACGC ATTGCTACTG CCTGCAGGGC 
CAGACGCTGT GCTCCACCGT GAGCTGCCCC GCCCTGCCCT GCCACGAGCC CGTCTACGTG 
GAGGGCAGCT GCTGTCCCAT GTGCTCAGGT GTGTATCCAC CCACGAACAT ACCCATCGAG 
AAGACAGACC GTCGGGGAGA TTCGCAGGTG CACCAGTCAG CCTGGCCAAC GAGGAGCGAG 
AATGACATCG TGCCCCAGTT CAGAGACTTC GGAAGCCTAC AGATGCCGTA CTTGGATGGC 
AAGGCACCGG TGCCCAGTGA AGAGGACACC TTCCACTCGG TGGCCTGGGT GGCGCTGCCC 
GTGCTCATGA TGCTCGTTAC TATTGCTATC CTGCTGCTCG TCAACCAGAA GAAGCAGTGG 
GTCCCCGTCC CCTGCTACCG GACCCCTACA AAGACCACCT GTCTGAACAA CCAGCTGGTG 
TACGTGGACT GTCAGAAGGG GACCAAGGTG CAGGTGGACA GCTCCCAGCG CGTGCTGCGG 
ATCGCCGACC CTGACTCCAG GCACAGCGGC TACTACAGCA TGCAGAAGCA GAACAACCTG 
CAGGCGGACA ACTTCTATCA GACTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018587848.1
CDS	788..3877
Translation	MASSRMYLLALKCIVISQLAVLMAKGSRALLCLPCDESKCEEPRHCRGAVVLGVCGCCSVCAKQRNESCGGVYGLYGTCDRGLRCVIRPALGGVSVTQYEVGVCEDENWDDDQLLGFEPCNENLITGCNIIDGKCECDSLRTCSNPFEFPSQGACQAALKKIEEEKPDCSSVRCEVQFSPRCPEDSVLIEGYAPPGECCPLPSRCVCSPAGCLRKVCQPGYLNILVSKGSGKPGECCDLYECKPVFSVDCRTVECPPVQQVQCPPDSYETQVRLTADGCCTLPTRCECLPGLCGFPQCSAGSVPRVISRGDGSPGRCCDVFECVNETKPPCSFNGVEYRDGDMFRMDACRFCRCQGGVSVCFQAQCGVLHCQRYYVPEGECCPVCEDTSYPALNYAGCFVNGQILAHGDHWREDDCTFCQCVSGEARCVAAACGHSCVNPVTVPGECCPVCEEPTYITMAPPACDSMKNCSLTEQDCIYGFKRDQDNCRICQCKTREELCRGLMADCTLDCPFGFQTDVHGCGVCQCRPRPKKCKPVSCDKECPFGFIKNKHGCEVCRCRKCPEVPCDKSCPFGFQLDRLGCLTCQCKDQSTSSAEPSVKLGSCLSMDGRRHDNGDNWHDGCRDCYCHNGREMCALISCPVPVCANPTIRPGQCCPSCADDPSSHKPELSEASVCLAPSGEYFVEGETWNIDSCTQCTCHSGRVLCETEVCPPLLCHSPIRTQDSCCPHCPDEPLTPQAPSNDSMPSYCRNEEGDIFLAAESWKPNMCSSCVCLDGVISCFSESCPVVGCARPVLRKGQCCPYCLEDTVPRAVCHFNGKTYADEERWDIDSCTHCYCLQGQTLCSTVSCPALPCHEPVYVEGSCCPMCSGVYPPTNIPIEKTDRRGDSQVHQSAWPTRSENDIVPQFRDFGSLQMPYLDGKAPVPSEEDTFHSVAWVALPVLMMLVTIAILLLVNQKKQWVPVPCYRTPTKTTCLNNQLVYVDCQKGTKVQVDSSQRVLRIADPDSRHSGYYSMQKQNNLQADNFYQTV

Target ORF information:

RefSeq Version	XM_018732332.2
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018732332.2

ORF Insert Sequence:

ATGGCCTCCT CCAGGATGTA CCTTCTCGCA CTGAAATGCA TCGTGATCTC GCAGCTCGCC 
GTGCTCATGG CCAAGGGCAG CCGCGCTCTG CTCTGCCTGC CGTGCGATGA GTCCAAGTGC 
GAGGAGCCGA GGCACTGCAG GGGCGCCGTG GTGCTCGGCG TGTGCGGCTG CTGCTCCGTG 
TGCGCCAAGC AGAGGAACGA GAGCTGCGGC GGAGTCTACG GGCTGTACGG GACGTGCGAT 
CGCGGGCTGC GCTGCGTCAT CCGACCGGCG CTCGGCGGAG TCTCCGTCAC GCAGTACGAG 
GTCGGCGTCT GCGAAGATGA GAACTGGGAT GATGACCAGC TTTTGGGCTT TGAGCCCTGC 
AATGAAAACC TCATCACGGG CTGCAACATC ATCGACGGGA AGTGCGAGTG CGACAGCCTG 
CGGACGTGCA GCAACCCTTT CGAGTTCCCC AGCCAGGGGG CCTGTCAGGC TGCGCTCAAG 
AAGATCGAAG AGGAGAAGCC GGACTGCTCC TCAGTGCGCT GCGAGGTCCA GTTCTCACCC 
CGCTGTCCTG AGGACTCCGT GCTGATCGAA GGCTACGCCC CCCCGGGTGA ATGTTGCCCG 
CTGCCCAGCC GCTGCGTTTG CAGTCCAGCC GGCTGCCTGC GCAAAGTGTG CCAGCCGGGT 
TACCTCAACA TCCTGGTGTC CAAGGGTTCG GGGAAGCCCG GCGAGTGTTG CGACCTGTAC 
GAATGCAAGC CAGTTTTCAG CGTGGACTGT AGAACGGTGG AGTGTCCGCC AGTCCAACAA 
GTTCAGTGCC CCCCTGACAG CTATGAGACC CAGGTCCGCC TCACCGCAGA TGGCTGCTGT 
ACCCTGCCAA CCAGATGTGA ATGTTTACCT GGCCTGTGTG GCTTCCCCCA GTGCTCCGCA 
GGAAGCGTTC CCCGTGTCAT TTCCCGCGGC GACGGCTCTC CGGGGAGGTG CTGCGACGTC 
TTTGAGTGCG TCAACGAGAC TAAGCCGCCA TGCTCCTTCA ATGGGGTGGA GTACCGAGAT 
GGAGACATGT TCCGGATGGA CGCCTGCCGC TTCTGCCGCT GCCAGGGCGG AGTGTCCGTC 
TGCTTCCAGG CACAATGTGG TGTCCTCCAC TGCCAGCGCT ACTACGTTCC CGAAGGAGAG 
TGCTGTCCCG TCTGTGAAGA CACCAGCTAC CCGGCTCTGA ATTATGCCGG ATGCTTTGTC 
AACGGTCAGA TCTTAGCTCA CGGCGACCAC TGGCGTGAGG ACGACTGCAC ATTCTGCCAG 
TGTGTGAGCG GGGAGGCACG GTGCGTGGCA GCTGCCTGCG GACACAGCTG TGTTAACCCC 
GTCACCGTGC CTGGGGAGTG CTGCCCTGTC TGCGAAGAGC CCACCTACAT CACCATGGCT 
CCCCCTGCTT GTGACTCCAT GAAAAACTGC AGCCTAACAG AGCAGGACTG TATCTACGGC 
TTCAAACGAG ACCAGGACAA CTGCCGCATC TGCCAGTGCA AGACACGAGA GGAGTTGTGC 
AGGGGCCTCA TGGCGGACTG CACCCTCGAC TGCCCCTTTG GTTTCCAAAC CGACGTCCAC 
GGATGCGGAG TATGTCAGTG TCGACCGAGG CCCAAAAAGT GCAAACCCGT GTCCTGCGAC 
AAGGAATGCC CCTTTGGCTT CATAAAGAAC AAGCATGGCT GTGAAGTGTG CCGCTGCAGG 
AAGTGCCCAG AGGTGCCATG CGACAAGTCC TGTCCATTTG GCTTCCAGCT TGACCGGCTG 
GGCTGCCTCA CCTGCCAGTG CAAAGACCAA AGCACTTCAT CTGCGGAACC CTCAGTGAAA 
CTGGGCTCCT GCTTGTCCAT GGATGGCCGT CGGCACGACA ACGGTGACAA CTGGCATGAC 
GGCTGTCGGG ACTGCTACTG CCACAATGGC AGGGAGATGT GCGCTCTGAT TTCGTGCCCT 
GTGCCCGTCT GCGCCAACCC TACCATTCGA CCTGGCCAGT GCTGCCCGTC TTGTGCAGAT 
GACCCCAGCT CCCACAAGCC TGAGCTCAGC GAGGCATCCG TGTGCCTGGC CCCCAGCGGG 
GAGTACTTTG TGGAGGGTGA AACTTGGAAC ATCGACTCAT GCACGCAGTG CACATGCCAC 
AGCGGCAGGG TGCTGTGTGA GACGGAGGTT TGCCCACCCC TGCTATGCCA CAGTCCCATC 
AGGACACAGG ACTCCTGCTG TCCACATTGC CCAGATGAGC CCCTCACTCC ACAGGCCCCC 
AGCAATGACA GCATGCCCAG CTACTGCCGC AATGAGGAGG GTGACATCTT CCTGGCAGCT 
GAGTCCTGGA AGCCCAACAT GTGCTCCAGT TGCGTCTGCC TGGACGGCGT CATCAGCTGC 
TTCTCCGAGT CCTGCCCGGT GGTGGGCTGC GCGAGGCCTG TGCTGCGCAA GGGCCAGTGC 
TGCCCGTACT GCCTAGAGGA CACGGTGCCA AGGGCGGTGT GCCACTTCAA TGGGAAGACA 
TACGCCGACG AGGAGCGCTG GGACATCGAC AGCTGCACGC ATTGCTACTG CCTGCAGGGC 
CAGACGCTGT GCTCCACCGT GAGCTGCCCC GCCCTGCCCT GCCACGAGCC CGTCTACGTG 
GAGGGCAGCT GCTGTCCCAT GTGCTCAGGT GTGTATCCAC CCACGAACAT ACCCATCGAG 
AAGACAGACC GTCGGGGAGA TTCGCAGGTG CACCAGTCAG CCTGGCCAAC GAGGAGCGAG 
AATGACATCG TGCCCCAGTT CAGAGACTTC GGAAGCCTAC AGATGCCGTA CTTGGATGGC 
AAGGCACCGG TGCCCAGTGA AGAGGACACC TTCCACTCGG TGGCCTGGGT GGCGCTGCCC 
GTGCTCATGA TGCTCGTTAC TATTGCTATC CTGCTGCTCG TCAACCAGAA GAAGCAGTGG 
GTCCCCGTCC CCTGCTACCG GACCCCTACA AAGACCACCT GTCTGAACAA CCAGCTGGTG 
TACGTGGACT GTCAGAAGGG GACCAAGGTG CAGGTGGACA GCTCCCAGCG CGTGCTGCGG 
ATCGCCGACC CTGACTCCAG GCACAGCGGC TACTACAGCA TGCAGAAGCA GAACAACCTG 
CAGGCGGACA ACTTCTATCA GACTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crim1 ( XM_018732332.1 ) cDNA ORF clone, Scleropages formosus(Asian bonytongue) -> XP_018587848.1 Scleropages formosus cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

Price & Availability↑

CloneID	OSc35172
Clone ID Related Accession (Same CDS sequence)	XM_018732332.1
Accession Version	XM_018732332.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Scleropages formosus(Asian bonytongue)
Product	cysteine-rich motor neuron 1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017371478.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Scleropages formosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCCT CCAGGATGTA CCTTCTCGCA CTGAAATGCA TCGTGATCTC GCAGCTCGCC 
GTGCTCATGG CCAAGGGCAG CCGCGCTCTG CTCTGCCTGC CGTGCGATGA GTCCAAGTGT 
GAGGAGCCGA GGCACTGCAG GGGCGCCGTG GTGCTCGGCG TGTGCGGCTG CTGCTCCGTG 
TGCGCCAAGC AGAGGAACGA GAGCTGCGGC GGAGTCTACG GGCTGTACGG GACGTGCGAT 
CGCGGGCTGC GCTGCGTCAT CCGACCGGCG CTCGGCGGAG TCTCCGTCAC GCAGTACGAG 
GTCGGCGTCT GCGAAGATGA GAACTGGGAT GATGACCAGC TTTTGGGGTT TGAGCCCTGC 
AATGAAAACC TCATCACGGG CTGCAACATC ATCGACGGGA AGTGCGAGTG CGACAGCCTG 
CGGACGTGCA GCAACCCTTT CGAGTTCCCC AGCCAGGGGG CCTGTCAGGC TGCGCTCAAG 
AAGATCGAAG AGGAGAAGCC GGACTGCTCC TCAGTGCGCT GCGAGGTCCA GTTCTCACCC 
CGCTGTCCTG AGGACTCCGT GCTGATCGAA GGCTACGCCC CCCCGGGTGA ATGTTGCCCG 
CTGCCCAGCC GCTGCGTTTG CAGTCCAGCC GGCTGCCTGC GCAAAGTGTG CCAGCCGGGT 
TACCTCAACA TCCTGGTGTC CAAGGGTTCG GGGAAGCCCG GCGAGTGTTG CGACCTGTAC 
GAATGCAAGC CAGTTTTCAG CGTGGACTGT AGAACGGTGG AGTGTCCGCC AGTCCAACAA 
GTTCAGTGCC CCCCTGACAG CTATGAGACC CAGGTCCGCC TCACCGCAGA TGGCTGCTGT 
ACCCTGCCAA CCAGATGTGA ATGTTTACCT GGCCTGTGTG GCTTCCCCCA GTGCTCCGCA 
GGAAGCGTTC CCCGTGTCAT TTCCCGCGGC GACGGCTCTC CGGGGAGGTG CTGCGACGTC 
TTTGAGTGCG TCAACGAGAC TAAGCCGCCA TGCTCCTTCA ATGGGGTGGA GTACCGAGAT 
GGAGACATGT TCCGGATGGA CGCCTGCCGC TTCTGCCGCT GCCAGGGCGG AGTGTCCGTC 
TGCTTCCAGG CACAATGTGG TGTCCTCCAC TGCCAGCGCT ACTACGTTCC CGAAGGAGAG 
TGCTGTCCCG TCTGTGAAGA CACCAGCTAC CCGGCTCTGA ATTATGCCGG ATGCTTTGTC 
AACGGTCAGA TCTTAGCTCA CGGCGACCAC TGGCGTGAGG ACGACTGCAC ATTCTGCCAG 
TGTGTGAGCG GGGAGGCACG GTGCGTGGCA GCTGCCTGCG GACACAGCTG TGTTAACCCC 
GTCACCGTGC CTGGGGAGTG CTGCCCTGTC TGCGAAGAGC CCACCTACAT CACCATGGCT 
CCCCCTGCTT GTGACTCCAT GAAAAACTGC AGCCTAACAG AGCAGGACTG TATCTACGGC 
TTCAAACGAG ACCAGGACAA CTGCCGCATC TGCCAGTGCA AGACACGAGA GGAGTTGTGC 
AGGGGCCTCA TGGCGGACTG CACCCTCGAC TGCCCCTTTG GTTTCCAAAC CGACGTCCAC 
GGATGCGGAG TATGTCAGTG TCGACCGAGG CCCAAAAAGT GCAAACCCGT GTCCTGCGAC 
AAGGAATGCC CCTTTGGCTT CATAAAGAAC AAGCATGGCT GTGAAGTGTG CCGCTGCAGG 
AAGTGCCCAG AGGTGCCATG CGACAAGTCC TGTCCATTTG GCTTCCAGCT TGACCGGCTG 
GGCTGCCTCA CCTGCCAGTG CAAAGACCAA AGCACTTCAT CTGCGGAACC CTCAGTGAAA 
CTGGGCTCCT GCTTGTCCAT GGATGGCCGT CGGCACGACA ACGGTGACAA CTGGCATGAC 
GGCTGTCGGG ACTGCTACTG CCACAATGGC AGGGAGATGT GCGCTCTGAT TTCGTGCCCT 
GTGCCCGTCT GCGCCAACCC TACCATTCGA CCTGGCCAGT GCTGCCCGTC TTGTGCAGAT 
GACCCCAGCT CCCACAAGCC TGAGCTCAGC GAGGCATCCG TGTGCCTGGC CCCCAGCGGG 
GAGTACTTTG TGGAGGGTGA AACTTGGAAC ATCGACTCAT GCACGCAGTG CACATGCCAC 
AGCGGCAGGG TGCTGTGTGA GACGGAGGTT TGCCCACCCC TGCTATGCCA CAGTCCCATC 
AGGACACAGG ACTCCTGCTG TCCACATTGC CCAGATGAGC CCCTCACTCC ACAGGCCCCC 
AGCAATGACA GCATGCCCAG CTACTGCCGC AATGAGGAGG GTGACATCTT CCTGGCAGCT 
GAGTCCTGGA AGCCCAACAT GTGCTCCAGT TGCGTCTGCC TGGACGGCGT CATCAGCTGC 
TTCTCCGAGT CCTGCCCGGT GGTGGGCTGC GCGAGGCCTG TGCTGCGCAA GGGCCAGTGC 
TGCCCGTACT GCCTAGAGGA CACGGTGCCA AGGGCGGTGT GCCACTTCAA TGGGAAGACA 
TACGCCGACG AGGAGCGCTG GGACATCGAC AGCTGCACGC ATTGCTACTG CCTGCAGGGC 
CAGACGCTGT GCTCCACCGT GAGCTGCCCC GCCCTGCCCT GCCACGAGCC CGTCTACGTG 
GAGGGCAGCT GCTGTCCCAT GTGCTCAGGT GTGTATCCAC CCACGAACAT ACCCATCGAG 
AAGACAGACC GTCGGGGAGA TTCGCAGGTG CACCAGTCAG CCTGGCCAAC GAGGAGCGAG 
AATGACATCG TGCCCCAGTT CAGAGACTTC GGAAGCCTAC AGATGCCGTA CTTGGATGGC 
AAGGCACCGG TGCCCAGTGA AGAGGACACC TTCCACTCGG TGGCCTGGGT GGCGCTGCCC 
GTGCTCATGA TGCTCGTTAC TATTGCTATC CTGCTGCTCG TCAACCAGAA GAAGCAGTGG 
GTCCCCGTCC CCTGCTACCG GACCCCTACA AAGACCACCT GTCTGAACAA CCAGCTGGTG 
TACGTGGACT GTCAGAAGGG GACCAAGGTG CAGGTGGACA GCTCCCAGCG CGTGCTGCGG 
ATCGCCGACC CTGACTCCAG GCACAGCGGC TACTACAGCA TGCAGAAGCA GAACAACCTG 
CAGGCGGACA ACTTCTATCA GACTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018587848.1
CDS	757..3846
Translation	MASSRMYLLALKCIVISQLAVLMAKGSRALLCLPCDESKCEEPRHCRGAVVLGVCGCCSVCAKQRNESCGGVYGLYGTCDRGLRCVIRPALGGVSVTQYEVGVCEDENWDDDQLLGFEPCNENLITGCNIIDGKCECDSLRTCSNPFEFPSQGACQAALKKIEEEKPDCSSVRCEVQFSPRCPEDSVLIEGYAPPGECCPLPSRCVCSPAGCLRKVCQPGYLNILVSKGSGKPGECCDLYECKPVFSVDCRTVECPPVQQVQCPPDSYETQVRLTADGCCTLPTRCECLPGLCGFPQCSAGSVPRVISRGDGSPGRCCDVFECVNETKPPCSFNGVEYRDGDMFRMDACRFCRCQGGVSVCFQAQCGVLHCQRYYVPEGECCPVCEDTSYPALNYAGCFVNGQILAHGDHWREDDCTFCQCVSGEARCVAAACGHSCVNPVTVPGECCPVCEEPTYITMAPPACDSMKNCSLTEQDCIYGFKRDQDNCRICQCKTREELCRGLMADCTLDCPFGFQTDVHGCGVCQCRPRPKKCKPVSCDKECPFGFIKNKHGCEVCRCRKCPEVPCDKSCPFGFQLDRLGCLTCQCKDQSTSSAEPSVKLGSCLSMDGRRHDNGDNWHDGCRDCYCHNGREMCALISCPVPVCANPTIRPGQCCPSCADDPSSHKPELSEASVCLAPSGEYFVEGETWNIDSCTQCTCHSGRVLCETEVCPPLLCHSPIRTQDSCCPHCPDEPLTPQAPSNDSMPSYCRNEEGDIFLAAESWKPNMCSSCVCLDGVISCFSESCPVVGCARPVLRKGQCCPYCLEDTVPRAVCHFNGKTYADEERWDIDSCTHCYCLQGQTLCSTVSCPALPCHEPVYVEGSCCPMCSGVYPPTNIPIEKTDRRGDSQVHQSAWPTRSENDIVPQFRDFGSLQMPYLDGKAPVPSEEDTFHSVAWVALPVLMMLVTIAILLLVNQKKQWVPVPCYRTPTKTTCLNNQLVYVDCQKGTKVQVDSSQRVLRIADPDSRHSGYYSMQKQNNLQADNFYQTV

Target ORF information:

RefSeq Version	XM_018732332.1
Organism	Scleropages formosus(Asian bonytongue)
Definition	Scleropages formosus cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018732332.1

ORF Insert Sequence:

ATGGCCTCCT CCAGGATGTA CCTTCTCGCA CTGAAATGCA TCGTGATCTC GCAGCTCGCC 
GTGCTCATGG CCAAGGGCAG CCGCGCTCTG CTCTGCCTGC CGTGCGATGA GTCCAAGTGT 
GAGGAGCCGA GGCACTGCAG GGGCGCCGTG GTGCTCGGCG TGTGCGGCTG CTGCTCCGTG 
TGCGCCAAGC AGAGGAACGA GAGCTGCGGC GGAGTCTACG GGCTGTACGG GACGTGCGAT 
CGCGGGCTGC GCTGCGTCAT CCGACCGGCG CTCGGCGGAG TCTCCGTCAC GCAGTACGAG 
GTCGGCGTCT GCGAAGATGA GAACTGGGAT GATGACCAGC TTTTGGGGTT TGAGCCCTGC 
AATGAAAACC TCATCACGGG CTGCAACATC ATCGACGGGA AGTGCGAGTG CGACAGCCTG 
CGGACGTGCA GCAACCCTTT CGAGTTCCCC AGCCAGGGGG CCTGTCAGGC TGCGCTCAAG 
AAGATCGAAG AGGAGAAGCC GGACTGCTCC TCAGTGCGCT GCGAGGTCCA GTTCTCACCC 
CGCTGTCCTG AGGACTCCGT GCTGATCGAA GGCTACGCCC CCCCGGGTGA ATGTTGCCCG 
CTGCCCAGCC GCTGCGTTTG CAGTCCAGCC GGCTGCCTGC GCAAAGTGTG CCAGCCGGGT 
TACCTCAACA TCCTGGTGTC CAAGGGTTCG GGGAAGCCCG GCGAGTGTTG CGACCTGTAC 
GAATGCAAGC CAGTTTTCAG CGTGGACTGT AGAACGGTGG AGTGTCCGCC AGTCCAACAA 
GTTCAGTGCC CCCCTGACAG CTATGAGACC CAGGTCCGCC TCACCGCAGA TGGCTGCTGT 
ACCCTGCCAA CCAGATGTGA ATGTTTACCT GGCCTGTGTG GCTTCCCCCA GTGCTCCGCA 
GGAAGCGTTC CCCGTGTCAT TTCCCGCGGC GACGGCTCTC CGGGGAGGTG CTGCGACGTC 
TTTGAGTGCG TCAACGAGAC TAAGCCGCCA TGCTCCTTCA ATGGGGTGGA GTACCGAGAT 
GGAGACATGT TCCGGATGGA CGCCTGCCGC TTCTGCCGCT GCCAGGGCGG AGTGTCCGTC 
TGCTTCCAGG CACAATGTGG TGTCCTCCAC TGCCAGCGCT ACTACGTTCC CGAAGGAGAG 
TGCTGTCCCG TCTGTGAAGA CACCAGCTAC CCGGCTCTGA ATTATGCCGG ATGCTTTGTC 
AACGGTCAGA TCTTAGCTCA CGGCGACCAC TGGCGTGAGG ACGACTGCAC ATTCTGCCAG 
TGTGTGAGCG GGGAGGCACG GTGCGTGGCA GCTGCCTGCG GACACAGCTG TGTTAACCCC 
GTCACCGTGC CTGGGGAGTG CTGCCCTGTC TGCGAAGAGC CCACCTACAT CACCATGGCT 
CCCCCTGCTT GTGACTCCAT GAAAAACTGC AGCCTAACAG AGCAGGACTG TATCTACGGC 
TTCAAACGAG ACCAGGACAA CTGCCGCATC TGCCAGTGCA AGACACGAGA GGAGTTGTGC 
AGGGGCCTCA TGGCGGACTG CACCCTCGAC TGCCCCTTTG GTTTCCAAAC CGACGTCCAC 
GGATGCGGAG TATGTCAGTG TCGACCGAGG CCCAAAAAGT GCAAACCCGT GTCCTGCGAC 
AAGGAATGCC CCTTTGGCTT CATAAAGAAC AAGCATGGCT GTGAAGTGTG CCGCTGCAGG 
AAGTGCCCAG AGGTGCCATG CGACAAGTCC TGTCCATTTG GCTTCCAGCT TGACCGGCTG 
GGCTGCCTCA CCTGCCAGTG CAAAGACCAA AGCACTTCAT CTGCGGAACC CTCAGTGAAA 
CTGGGCTCCT GCTTGTCCAT GGATGGCCGT CGGCACGACA ACGGTGACAA CTGGCATGAC 
GGCTGTCGGG ACTGCTACTG CCACAATGGC AGGGAGATGT GCGCTCTGAT TTCGTGCCCT 
GTGCCCGTCT GCGCCAACCC TACCATTCGA CCTGGCCAGT GCTGCCCGTC TTGTGCAGAT 
GACCCCAGCT CCCACAAGCC TGAGCTCAGC GAGGCATCCG TGTGCCTGGC CCCCAGCGGG 
GAGTACTTTG TGGAGGGTGA AACTTGGAAC ATCGACTCAT GCACGCAGTG CACATGCCAC 
AGCGGCAGGG TGCTGTGTGA GACGGAGGTT TGCCCACCCC TGCTATGCCA CAGTCCCATC 
AGGACACAGG ACTCCTGCTG TCCACATTGC CCAGATGAGC CCCTCACTCC ACAGGCCCCC 
AGCAATGACA GCATGCCCAG CTACTGCCGC AATGAGGAGG GTGACATCTT CCTGGCAGCT 
GAGTCCTGGA AGCCCAACAT GTGCTCCAGT TGCGTCTGCC TGGACGGCGT CATCAGCTGC 
TTCTCCGAGT CCTGCCCGGT GGTGGGCTGC GCGAGGCCTG TGCTGCGCAA GGGCCAGTGC 
TGCCCGTACT GCCTAGAGGA CACGGTGCCA AGGGCGGTGT GCCACTTCAA TGGGAAGACA 
TACGCCGACG AGGAGCGCTG GGACATCGAC AGCTGCACGC ATTGCTACTG CCTGCAGGGC 
CAGACGCTGT GCTCCACCGT GAGCTGCCCC GCCCTGCCCT GCCACGAGCC CGTCTACGTG 
GAGGGCAGCT GCTGTCCCAT GTGCTCAGGT GTGTATCCAC CCACGAACAT ACCCATCGAG 
AAGACAGACC GTCGGGGAGA TTCGCAGGTG CACCAGTCAG CCTGGCCAAC GAGGAGCGAG 
AATGACATCG TGCCCCAGTT CAGAGACTTC GGAAGCCTAC AGATGCCGTA CTTGGATGGC 
AAGGCACCGG TGCCCAGTGA AGAGGACACC TTCCACTCGG TGGCCTGGGT GGCGCTGCCC 
GTGCTCATGA TGCTCGTTAC TATTGCTATC CTGCTGCTCG TCAACCAGAA GAAGCAGTGG 
GTCCCCGTCC CCTGCTACCG GACCCCTACA AAGACCACCT GTCTGAACAA CCAGCTGGTG 
TACGTGGACT GTCAGAAGGG GACCAAGGTG CAGGTGGACA GCTCCCAGCG CGTGCTGCGG 
ATCGCCGACC CTGACTCCAG GCACAGCGGC TACTACAGCA TGCAGAAGCA GAACAACCTG 
CAGGCGGACA ACTTCTATCA GACTGTGTGA