EIF3A cDNA ORF clone, Saccoglossus kowalevskii

Gene
Clones
gRNAs

Summary

Gene Symbol	EIF3A
Entrez Gene ID	100377637
Full Name	eukaryotic translation initiation factor 3, subunit A
Gene Type	protein-coding
Organism	Saccoglossus kowalevskii

mRNA and Protein(s)

mRNA	Protein	Name
XM_002738360.2	XP_002738406.1	eukaryotic translation initiation factor 3 subunit A

Pathways from BioSystems
Gene Ontology

The following EIF3A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF3A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSg10871	XM_002738360.2 Latest version!	Saccoglossus kowalevskii eukaryotic translation initiation factor 3, subunit A (EIF3A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EIF3A ( XM_002738360.2 ) cDNA ORF clone, Saccoglossus kowalevskii -> XP_002738406.1 Saccoglossus kowalevskii eukaryotic translation initiation factor 3, subunit A (EIF3A), mRNA.

Price & Availability↑

CloneID	OSg10871
Clone ID Related Accession (Same CDS sequence)	XM_002738360.2
Accession Version	XM_002738360.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-02-17
Organism	Saccoglossus kowalevskii
Product	eukaryotic translation initiation factor 3 subunit A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003140812.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 18, 2014 this sequence version replaced XM_002738360.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Saccoglossus kowalevskii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTACAT ACTTTCAGCG TCCCGAAAAT GCTCTCAAAA GAGCAAACGA ATTCATCGAT 
GTTGGTAAAA AGCAGCCTGC ATTGGATGCC TTGTATGATG TTATCAAGAG CAAAAAACAT 
CGAACATGGC AGAAAATCCA TGAACCGATC ATGGAGAAAT ATCTAGTGCT GTGTGTTGAT 
TTACGCAAGA GTCATATTGC AAAGGAAGGT CTTTATCAAT ACAAGAATAT CTGTCAACAG 
GTTAACATCA GATCCCTAGA GGATGTTGTT AGAAGTTATT TAAGACTGGC CGAAGAAAAA 
ACTGAACAGG CCAAGGAAGA ATCGCACCAG AAAGTTGATG ATATTGATGA CTTAGATCAA 
ATCACAACAC CAGAGAGCTT ACTTCTAAGT GCTGTGAGTG GTGAGGATAC ACAAGATCGA 
ACTGATCGCA TACTTCTGAC ACCGTGGGTA AAGTTCCTAT GGGAGTCATA TCGACAGTGT 
TTGGATTTAC TAAGAAATAA TTCCAAAGTT GAAAGGCTTT ATCAAGATAT AGCTCAACAA 
GCTTTCAAGT TCTGTTTGAA ATACTCCAGG AAGACGGAAT TTCGTAAACT ATGTGAAAAT 
CTAAGGAATC ATCTTGGGTT GATTACAAAA TATCAGAACC AAGCATCGTC TATTAATTTG 
AACAATCCTG ACAGTCAGCA GATGCATCTG GAAACCAGAT TAGTGCAGTT AGACAGTGCT 
ATCAGTATGG AACTATGGCA GGAAGCCTAC AAAGCCATTG AAGATGTACA TGGGCTGATG 
ACGTTGTCTA AGAAATCCCC TAAACCTCAG CTGATGGCTA ACTATTACAC CAAGCTGGGT 
TTAGTGTTTT GGAAATCTGG AAATCATCTC TTCCATGCAT GTGCTTTGCA TCGTTTATTA 
CAATTGTCGA GAGAACAGCG GAAGAATCTC TCGCAGGACG AGATTCAAAA AATGGCATCC 
CGTGTGTTAC TAGCTACTTT ATCTATTCAA GTTATCCAAC CTAGAAATGA AATCACTACA 
ATCCTGGACA TGGAGGAAGC ACATATTGAA AAACAACGTA AACTAGCCAA CCTATTAGGA 
TTGCAGAGTC CACCCAATAG ATCTCAATTA ATCAAAGATA TGGTCAAGTA CAACGTCATC 
CAGCATGTAT TGCCACAGTT AAAAGATCTA TTTAATTGGT TAGAAGTAGA GTTCCATCCG 
TTACAGCTAA GTGATAGAGT GACTAAAGTA CTAGAATTCA TAGAGGAAAC TAACGAATTA 
GAGATGGTGC AGTATGTGTC GGCACTGCAA AATGTCGCTC TTACCAGATT ACTTTCACAG 
GTGTCTCAAG TGTACCAAAC AATTCAGTTT AGTCGTCTAT CTCAGCTGGC TCCGTTTGCT 
TCGGCGTTCC ATTTGGAGAA TGTTATCGTT GATGCAGCTA GGAGTGGTGC ATTACAGGTA 
CAAATAGATC ATCGTAATCG TTCACTTCGT TTTGGATCTG ATCTTGGCAT GTCAACCCTG 
GAACCGGACG AGGAAGTAGA TGAAGGTCCT TATTTACAAG CTATGCCGTC GGATCAAATC 
AGAAACCAAT TGACTAAAAT GTCTGCAGCT TTAATCAAAG CTGTTGCATA CGTCAGTCCA 
AGTCACAAAC AGTCTGCTCG AGCCGATCAA CGCAAGCAGA TAGTGTCAAG TTATATGCGT 
AATTGTAAGA AGGAACACAG TCGAATACTT GCTAGGAGGC AGATCATTGA AGACAGAAAA 
GAGCACCTTG AAAGTCTAAA TACACAAAGA GAAAAGCAGG AGCAAGACTT ACTAGAACAG 
CAAAAACGTG TTGCCGTGGA AGCAGAGAAA CGTAGACTTG AGAAAGAAGC TGAGGAGAGA 
GAACATAGAC GAAGAAAAGA GGAACATAAA ATCATTCAAC AGAAGCAGAC TTTAGAGCGA 
ATTGAGAATT TGAAAAAGAC TGCTATTGGT GCTCGTACAT TAAAGAAACT TGACTTAGAT 
GAATTGAAAG AGATGGATGC AGATGAAATC ATGGCTAAAC AAGTTGAGCA GTTGGAGCAA 
GAGAAAAAAG AACTGCAAAC GCGATTGAAA GCACAGGAAA AAAGGATTGA TTACTTTGCC 
CGTGCACAGC GACTTGAAGA GATCCCGCTT CTTAAAAAAG ATTATGAAGA GTTTGCCTTG 
ATGGACAGAG AAACCTGGGA TAATGAAGAA GAGAAGAGAA TCCAGAATTT GAAAGAAGAG 
CGGGAAATGT CCAAGTTGGA GCGAAATCGT TTGGCCTTTA TGGCAGTTGA TAAAGACGAA 
TTCCTAAATG GTTTGACCCA AGCCAGAATG TCTGTCTATA AGGAAAAACT AGAAAACTTC 
AAGACTATGT ACGAGGAAGA AAGGAAAGTA CGACTGGAAG AGCGGAAAGA CAGGCGTAAA 
GAAGAACGCC GACAGAAGTG GTTAAAAGAA AAGGAAGAGG AGGAGCAGCG TAAACGTGAT 
GAGGAAATGA AAAGAGAAAG GGAACGAGAA GAAGAGGAGA AACGAGCCAA ACAGGAGGAA 
GAAGAGGAAG AATACAGAGA CAGACTTGCT AAATTGGAGG AACAAGCTGC TAAACAGAGA 
GCAAGAGAGA AAGAAATTGA AGAAAGACTG GCCAGAAGAG AAATGACTTC CAGAGATAGG 
TCTGAACCAG AAAGACGAGA TGCTGGTTGG AGACATGAAG AACGAAAAGA TGATGATTCT 
GGCCCATGGA GAGCTCCAGT GCCAAGTGAA CCTGCTAGCG ACCGACCAAC TCGGGCCTGG 
AAACCTCCAT CCAGGGATGG TGGAGGATGG AGAGCCAGAG AAATGGACAG GGCTGGTGTA 
CAGAGGAGGG ATGCAAGATC TCCTCCTTTT CAAGATAGGC CTTCCCTGGA TGATGCACCA 
GCTCGTGACA GGCTACCAGC TCGTGATGAG AGATACAGTG ATATAAGACG AGAAGATGGA 
CCTTGGAGAG GGCGGGACGA CCTTGACAGA CGTGGCCCTC CACCTGAAGA TAGAGACAAA 
GCTTGGGCAC GTAAGGACGA TCGTGATAGA TCTGGTCCAG CCTTAGACAG AGAAAGTGAT 
TGGCGACGAG GACCTCCACC TGATGACCGT GATAGACGAC CCCCGCTTGA TGACCGTGAT 
AGACGAGGAC CTCCGTTTGA TGATCGCGAT AGACGGGGAC CTGATGCATG GAGACGTGAT 
GATCGTGGTT CTCCCAGCAG AGGTCCACCC CCAAGAGATG ACAGAGGACC CTGGAGAGGA 
TCTTCCAGAG ATGATAGCGG ACCTGCCTGG CGTCGAGAAG AACGTGATAG GGGCCCACCT 
GTGAAAGAAG ATCGCTATAT TCCACCACGT GGAGATGAAG AGCGAGATTG GCGTAGAGAT 
GACCGTGGAC CTATAAGAGA TGATAGAGAT CGTGAACCTC CAAATGCTTG GAGGCGCGAC 
CGCGGCCCTC TTGTACGCGG TGACAGAGAC AGGCCACCGA TGCGTGATGA CAGAGACAGG 
CCACCAATAC GTGATGACAG AGACAGGCCA CCAATACGTG ATGACAGAGA CAGGCCACCA 
ATACGTGATG ACAGAGACAG GCCACCGATG CGTGATGACC GAGACAGGCC ACCACTGCGA 
GGTGATAGGG ACATTCGTGG CCCACCAGAT GATCGTTGGC GTGACGGACC CCAAAGAGAA 
AATGAATGGA GACGCGGAGA TGACAGACCA AGTGATTCTA GACTACCTCC TAGAGATAAA 
GGTCCACCAC CACGCAGTGA CAGAGATGAG AAATGGCAAA GAAGTAGAGA AGAACCTGAA 
AGAAATAAAG AAGATGACGG TTGGCAAACT GTTTCTAGGC GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002738406.1
CDS	117..3941
Translation	MPTYFQRPENALKRANEFIDVGKKQPALDALYDVIKSKKHRTWQKIHEPIMEKYLVLCVDLRKSHIAKEGLYQYKNICQQVNIRSLEDVVRSYLRLAEEKTEQAKEESHQKVDDIDDLDQITTPESLLLSAVSGEDTQDRTDRILLTPWVKFLWESYRQCLDLLRNNSKVERLYQDIAQQAFKFCLKYSRKTEFRKLCENLRNHLGLITKYQNQASSINLNNPDSQQMHLETRLVQLDSAISMELWQEAYKAIEDVHGLMTLSKKSPKPQLMANYYTKLGLVFWKSGNHLFHACALHRLLQLSREQRKNLSQDEIQKMASRVLLATLSIQVIQPRNEITTILDMEEAHIEKQRKLANLLGLQSPPNRSQLIKDMVKYNVIQHVLPQLKDLFNWLEVEFHPLQLSDRVTKVLEFIEETNELEMVQYVSALQNVALTRLLSQVSQVYQTIQFSRLSQLAPFASAFHLENVIVDAARSGALQVQIDHRNRSLRFGSDLGMSTLEPDEEVDEGPYLQAMPSDQIRNQLTKMSAALIKAVAYVSPSHKQSARADQRKQIVSSYMRNCKKEHSRILARRQIIEDRKEHLESLNTQREKQEQDLLEQQKRVAVEAEKRRLEKEAEEREHRRRKEEHKIIQQKQTLERIENLKKTAIGARTLKKLDLDELKEMDADEIMAKQVEQLEQEKKELQTRLKAQEKRIDYFARAQRLEEIPLLKKDYEEFALMDRETWDNEEEKRIQNLKEEREMSKLERNRLAFMAVDKDEFLNGLTQARMSVYKEKLENFKTMYEEERKVRLEERKDRRKEERRQKWLKEKEEEEQRKRDEEMKREREREEEEKRAKQEEEEEEYRDRLAKLEEQAAKQRAREKEIEERLARREMTSRDRSEPERRDAGWRHEERKDDDSGPWRAPVPSEPASDRPTRAWKPPSRDGGGWRAREMDRAGVQRRDARSPPFQDRPSLDDAPARDRLPARDERYSDIRREDGPWRGRDDLDRRGPPPEDRDKAWARKDDRDRSGPALDRESDWRRGPPPDDRDRRPPLDDRDRRGPPFDDRDRRGPDAWRRDDRGSPSRGPPPRDDRGPWRGSSRDDSGPAWRREERDRGPPVKEDRYIPPRGDEERDWRRDDRGPIRDDRDREPPNAWRRDRGPLVRGDRDRPPMRDDRDRPPIRDDRDRPPIRDDRDRPPIRDDRDRPPMRDDRDRPPLRGDRDIRGPPDDRWRDGPQRENEWRRGDDRPSDSRLPPRDKGPPPRSDRDEKWQRSREEPERNKEDDGWQTVSRR

Target ORF information:

RefSeq Version	XM_002738360.2
Organism	Saccoglossus kowalevskii
Definition	Saccoglossus kowalevskii eukaryotic translation initiation factor 3, subunit A (EIF3A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002738360.2

ORF Insert Sequence:

ATGCCTACAT ACTTTCAGCG TCCCGAAAAT GCTCTCAAAA GAGCAAACGA ATTCATCGAT 
GTTGGTAAAA AGCAGCCTGC ATTGGATGCC TTGTATGATG TTATCAAGAG CAAAAAACAT 
CGAACATGGC AGAAAATCCA TGAACCGATC ATGGAGAAAT ATCTAGTGCT GTGTGTTGAT 
TTACGCAAGA GTCATATTGC AAAGGAAGGT CTTTATCAAT ACAAGAATAT CTGTCAACAG 
GTTAACATCA GATCCCTAGA GGATGTTGTT AGAAGTTATT TAAGACTGGC CGAAGAAAAA 
ACTGAACAGG CCAAGGAAGA ATCGCACCAG AAAGTTGATG ATATTGATGA CTTAGATCAA 
ATCACAACAC CAGAGAGCTT ACTTCTAAGT GCTGTGAGTG GTGAGGATAC ACAAGATCGA 
ACTGATCGCA TACTTCTGAC ACCGTGGGTA AAGTTCCTAT GGGAGTCATA TCGACAGTGT 
TTGGATTTAC TAAGAAATAA TTCCAAAGTT GAAAGGCTTT ATCAAGATAT AGCTCAACAA 
GCTTTCAAGT TCTGTTTGAA ATACTCCAGG AAGACGGAAT TTCGTAAACT ATGTGAAAAT 
CTAAGGAATC ATCTTGGGTT GATTACAAAA TATCAGAACC AAGCATCGTC TATTAATTTG 
AACAATCCTG ACAGTCAGCA GATGCATCTG GAAACCAGAT TAGTGCAGTT AGACAGTGCT 
ATCAGTATGG AACTATGGCA GGAAGCCTAC AAAGCCATTG AAGATGTACA TGGGCTGATG 
ACGTTGTCTA AGAAATCCCC TAAACCTCAG CTGATGGCTA ACTATTACAC CAAGCTGGGT 
TTAGTGTTTT GGAAATCTGG AAATCATCTC TTCCATGCAT GTGCTTTGCA TCGTTTATTA 
CAATTGTCGA GAGAACAGCG GAAGAATCTC TCGCAGGACG AGATTCAAAA AATGGCATCC 
CGTGTGTTAC TAGCTACTTT ATCTATTCAA GTTATCCAAC CTAGAAATGA AATCACTACA 
ATCCTGGACA TGGAGGAAGC ACATATTGAA AAACAACGTA AACTAGCCAA CCTATTAGGA 
TTGCAGAGTC CACCCAATAG ATCTCAATTA ATCAAAGATA TGGTCAAGTA CAACGTCATC 
CAGCATGTAT TGCCACAGTT AAAAGATCTA TTTAATTGGT TAGAAGTAGA GTTCCATCCG 
TTACAGCTAA GTGATAGAGT GACTAAAGTA CTAGAATTCA TAGAGGAAAC TAACGAATTA 
GAGATGGTGC AGTATGTGTC GGCACTGCAA AATGTCGCTC TTACCAGATT ACTTTCACAG 
GTGTCTCAAG TGTACCAAAC AATTCAGTTT AGTCGTCTAT CTCAGCTGGC TCCGTTTGCT 
TCGGCGTTCC ATTTGGAGAA TGTTATCGTT GATGCAGCTA GGAGTGGTGC ATTACAGGTA 
CAAATAGATC ATCGTAATCG TTCACTTCGT TTTGGATCTG ATCTTGGCAT GTCAACCCTG 
GAACCGGACG AGGAAGTAGA TGAAGGTCCT TATTTACAAG CTATGCCGTC GGATCAAATC 
AGAAACCAAT TGACTAAAAT GTCTGCAGCT TTAATCAAAG CTGTTGCATA CGTCAGTCCA 
AGTCACAAAC AGTCTGCTCG AGCCGATCAA CGCAAGCAGA TAGTGTCAAG TTATATGCGT 
AATTGTAAGA AGGAACACAG TCGAATACTT GCTAGGAGGC AGATCATTGA AGACAGAAAA 
GAGCACCTTG AAAGTCTAAA TACACAAAGA GAAAAGCAGG AGCAAGACTT ACTAGAACAG 
CAAAAACGTG TTGCCGTGGA AGCAGAGAAA CGTAGACTTG AGAAAGAAGC TGAGGAGAGA 
GAACATAGAC GAAGAAAAGA GGAACATAAA ATCATTCAAC AGAAGCAGAC TTTAGAGCGA 
ATTGAGAATT TGAAAAAGAC TGCTATTGGT GCTCGTACAT TAAAGAAACT TGACTTAGAT 
GAATTGAAAG AGATGGATGC AGATGAAATC ATGGCTAAAC AAGTTGAGCA GTTGGAGCAA 
GAGAAAAAAG AACTGCAAAC GCGATTGAAA GCACAGGAAA AAAGGATTGA TTACTTTGCC 
CGTGCACAGC GACTTGAAGA GATCCCGCTT CTTAAAAAAG ATTATGAAGA GTTTGCCTTG 
ATGGACAGAG AAACCTGGGA TAATGAAGAA GAGAAGAGAA TCCAGAATTT GAAAGAAGAG 
CGGGAAATGT CCAAGTTGGA GCGAAATCGT TTGGCCTTTA TGGCAGTTGA TAAAGACGAA 
TTCCTAAATG GTTTGACCCA AGCCAGAATG TCTGTCTATA AGGAAAAACT AGAAAACTTC 
AAGACTATGT ACGAGGAAGA AAGGAAAGTA CGACTGGAAG AGCGGAAAGA CAGGCGTAAA 
GAAGAACGCC GACAGAAGTG GTTAAAAGAA AAGGAAGAGG AGGAGCAGCG TAAACGTGAT 
GAGGAAATGA AAAGAGAAAG GGAACGAGAA GAAGAGGAGA AACGAGCCAA ACAGGAGGAA 
GAAGAGGAAG AATACAGAGA CAGACTTGCT AAATTGGAGG AACAAGCTGC TAAACAGAGA 
GCAAGAGAGA AAGAAATTGA AGAAAGACTG GCCAGAAGAG AAATGACTTC CAGAGATAGG 
TCTGAACCAG AAAGACGAGA TGCTGGTTGG AGACATGAAG AACGAAAAGA TGATGATTCT 
GGCCCATGGA GAGCTCCAGT GCCAAGTGAA CCTGCTAGCG ACCGACCAAC TCGGGCCTGG 
AAACCTCCAT CCAGGGATGG TGGAGGATGG AGAGCCAGAG AAATGGACAG GGCTGGTGTA 
CAGAGGAGGG ATGCAAGATC TCCTCCTTTT CAAGATAGGC CTTCCCTGGA TGATGCACCA 
GCTCGTGACA GGCTACCAGC TCGTGATGAG AGATACAGTG ATATAAGACG AGAAGATGGA 
CCTTGGAGAG GGCGGGACGA CCTTGACAGA CGTGGCCCTC CACCTGAAGA TAGAGACAAA 
GCTTGGGCAC GTAAGGACGA TCGTGATAGA TCTGGTCCAG CCTTAGACAG AGAAAGTGAT 
TGGCGACGAG GACCTCCACC TGATGACCGT GATAGACGAC CCCCGCTTGA TGACCGTGAT 
AGACGAGGAC CTCCGTTTGA TGATCGCGAT AGACGGGGAC CTGATGCATG GAGACGTGAT 
GATCGTGGTT CTCCCAGCAG AGGTCCACCC CCAAGAGATG ACAGAGGACC CTGGAGAGGA 
TCTTCCAGAG ATGATAGCGG ACCTGCCTGG CGTCGAGAAG AACGTGATAG GGGCCCACCT 
GTGAAAGAAG ATCGCTATAT TCCACCACGT GGAGATGAAG AGCGAGATTG GCGTAGAGAT 
GACCGTGGAC CTATAAGAGA TGATAGAGAT CGTGAACCTC CAAATGCTTG GAGGCGCGAC 
CGCGGCCCTC TTGTACGCGG TGACAGAGAC AGGCCACCGA TGCGTGATGA CAGAGACAGG 
CCACCAATAC GTGATGACAG AGACAGGCCA CCAATACGTG ATGACAGAGA CAGGCCACCA 
ATACGTGATG ACAGAGACAG GCCACCGATG CGTGATGACC GAGACAGGCC ACCACTGCGA 
GGTGATAGGG ACATTCGTGG CCCACCAGAT GATCGTTGGC GTGACGGACC CCAAAGAGAA 
AATGAATGGA GACGCGGAGA TGACAGACCA AGTGATTCTA GACTACCTCC TAGAGATAAA 
GGTCCACCAC CACGCAGTGA CAGAGATGAG AAATGGCAAA GAAGTAGAGA AGAACCTGAA 
AGAAATAAAG AAGATGACGG TTGGCAAACT GTTTCTAGGC GCTAA