PPH3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PPH3
Entrez Gene ID	851647
Full Name	phosphoprotein phosphatase PP4 catalytic subunit PPH3
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180383.1	NP_010360.1	phosphoprotein phosphatase PP4 catalytic subunit PPH3

Pathways from BioSystems

Homologies

	PPP4C	XP_001108500.1
Canis lupus familiaris (dog)	PPP4C	XP_547067.1
Rattus norvegicus (Norway rat)	Ppp4c	NP_599186.1
Danio rerio (zebrafish)	ppp4cb	NP_001104638.1
Kluyveromyces lactis	KLLA0E10055g	XP_454403.1
Drosophila melanogaster (fruit fly)	Pp4-19C	NP_524803.1
Neurospora crassa	NCU08301	XP_962514.2
Arabidopsis thaliana (thale cress)	PPX1	NP_194402.1
Oryza sativa (rice)	Os09g0284200	NP_001062774.1
Pan troglodytes (chimpanzee)	PPP4C	XP_001148577.1
Eremothecium gossypii	AGOS_AGR136W	NP_986802.2
Schizosaccharomyces pombe (fission yeast)	SPBC26H8.05c	NP_596646.1
Xenopus tropicalis (tropical clawed frog)	ppp4c	NP_988943.1
Bos taurus (cattle)	PPP4C	NP_001092578.1
Mus musculus (house mouse)	Ppp4c	NP_062648.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003779	XP_310323.5
Caenorhabditis elegans (roundworm)	pph-4.1	NP_499603.1
Homo sapiens (human)	PPP4C	NP_002711.1
Saccharomyces cerevisiae (baker's yeast)	PPH3	NP_010360.1
Magnaporthe oryzae (rice blast fungus)	MGG_01528	XP_003714501.1
Arabidopsis thaliana (thale cress)	PPX2	NP_200337.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Physical interaction between Pph3 and Mec1, mediated by cofactors Psy2 and Ddc2, is important for regulating the checkpoint response in yeast.
Title: Yeast PP4 interacts with ATR homolog Ddc2-Mec1 and regulates checkpoint signaling.

Data show that Pph3-Psy2 targets the glucose signal transducer protein Mth1, which is required for the dephosphorylation of Rgt1 upon glucose withdrawal, a critical event in the repression of HXT genes, which encode glucose transporters.
Title: Psy2 targets the PP4 family phosphatase Pph3 to dephosphorylate Mth1 and repress glucose transporter gene expression.

Data indicate that the phosphatase complex Pph3/Psy2 connects homologous end-joining (NHEJ) repair to cell cycle progression.
Title: Phosphatase complex Pph3/Psy2 is involved in regulation of efficient non-homologous end-joining pathway in the yeast Saccharomyces cerevisiae.

Results suggest that the trimeric Pph3p-Psy4p-Psy2p complex may dephosphorylate both gammaH2AX and Rad53p.
Title: Characterization of the role of a trimeric protein phosphatase complex in recovery from cisplatin-induced versus noncrosslinking DNA damage.

Mutational analyses of conserved regions of Tap42/alpha4 identified a region critical for its interactions with PP2Ac. We propose a scaffolding function for Tap42/alpha4 whereby the interaction of PP2Ac promotes the dephosphorylation of substrates.
Title: The structure of Tap42/alpha4 reveals a tetratricopeptide repeat-like fold and provides insights into PP2A regulation.

The following PPH3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPH3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01164	NM_001180383.1 Latest version!	Saccharomyces cerevisiae S288C phosphoprotein phosphatase PP4 catalytic subunit PPH3 (PPH3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPH3 ( NM_001180383.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010360.1 Saccharomyces cerevisiae S288C phosphoprotein phosphatase PP4 catalytic subunit PPH3 (PPH3), partial mRNA.

Price & Availability↑

CloneID	OSi01164
Clone ID Related Accession (Same CDS sequence)	NM_001180383.1
Accession Version	NM_001180383.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	phosphoprotein phosphatase PP4 catalytic subunit PPH3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGATGGACT TAGATAAGAT TATAGCATCA CTGAGAGACG GAAAACATAT TCCTGAAGAA 
ACCGTTTTTA GGCTATGTTT AAATTCACAG GAACTATTAA TGAATGAAGG CAATGTAACA 
CAAGTCGATA CACCGGTTAC AATATGCGGT GATATACATG GCCAATTACA CGATCTACTA 
ACGCTCTTCG AAAAGAGTGG TGGTGTAGAG AAAACAAGGT ATATTTTCTT GGGCGATTTT 
GTGGATAGGG GATTTTACTC ATTGGAGAGT TTTTTACTTT TACTATGTTA CAAATTAAGA 
TATCCTGATA GGATTACTTT AATTAGAGGC AATCACGAAA CCCGGCAAAT TACTAAAGTA 
TACGGATTTT ACGATGAAGT AGTAAGAAAA TATGGTAATA GTAACGTATG GAGGTACTGC 
TGTGAAGTTT TTGATTATTT ATCATTGGGG GCAATAATAA ACAATAGCAT ATTCTGTGTT 
CATGGTGGAT TATCTCCGGA TATGACCACG GTTGATGAAA TACGAACAAT AGACAGGAAA 
CAAGAAGTTC CACATGAAGG TGCTATGTGT GACTTATTAT GGAGCGACCC CGAAGACGTT 
GACACATGGT CATTATCACC AAGAGGTGCT GGATTTCTTT TCGGTAAAAG AGAAGTTGAT 
CAATTCTTAG AGAAAAACAA CGTTGAGTTA ATTGCTAGAG CTCATCAGTT AGTGATGGAA 
GGTTACAAAG AAATGTTCGA CGGTGGATTA GTGACAGTCT GGTCGGCACC GAATTACTGT 
TATCGTTGTG GTAATGTAGC AGCTGTATTG AAGATAGACG ACGATTTGAA TCGTGAATAT 
ACAATTTTTG AAGCTGTTCA GGCACAAAAT GAAGTCGGAA ATGCTATAAT TCCAACCAAA 
AAATCTCAAA TGGACTATTT CTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010360.1
CDS	1..927
Translation	MMDLDKIIASLRDGKHIPEETVFRLCLNSQELLMNEGNVTQVDTPVTICGDIHGQLHDLLTLFEKSGGVEKTRYIFLGDFVDRGFYSLESFLLLLCYKLRYPDRITLIRGNHETRQITKVYGFYDEVVRKYGNSNVWRYCCEVFDYLSLGAIINNSIFCVHGGLSPDMTTVDEIRTIDRKQEVPHEGAMCDLLWSDPEDVDTWSLSPRGAGFLFGKREVDQFLEKNNVELIARAHQLVMEGYKEMFDGGLVTVWSAPNYCYRCGNVAAVLKIDDDLNREYTIFEAVQAQNEVGNAIIPTKKSQMDYFL

Target ORF information:

RefSeq Version	NM_001180383.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C phosphoprotein phosphatase PP4 catalytic subunit PPH3 (PPH3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180383.1

ORF Insert Sequence:

ATGATGGACT TAGATAAGAT TATAGCATCA CTGAGAGACG GAAAACATAT TCCTGAAGAA 
ACCGTTTTTA GGCTATGTTT AAATTCACAG GAACTATTAA TGAATGAAGG CAATGTAACA 
CAAGTCGATA CACCGGTTAC AATATGCGGT GATATACATG GCCAATTACA CGATCTACTA 
ACGCTCTTCG AAAAGAGTGG TGGTGTAGAG AAAACAAGGT ATATTTTCTT GGGCGATTTT 
GTGGATAGGG GATTTTACTC ATTGGAGAGT TTTTTACTTT TACTATGTTA CAAATTAAGA 
TATCCTGATA GGATTACTTT AATTAGAGGC AATCACGAAA CCCGGCAAAT TACTAAAGTA 
TACGGATTTT ACGATGAAGT AGTAAGAAAA TATGGTAATA GTAACGTATG GAGGTACTGC 
TGTGAAGTTT TTGATTATTT ATCATTGGGG GCAATAATAA ACAATAGCAT ATTCTGTGTT 
CATGGTGGAT TATCTCCGGA TATGACCACG GTTGATGAAA TACGAACAAT AGACAGGAAA 
CAAGAAGTTC CACATGAAGG TGCTATGTGT GACTTATTAT GGAGCGACCC CGAAGACGTT 
GACACATGGT CATTATCACC AAGAGGTGCT GGATTTCTTT TCGGTAAAAG AGAAGTTGAT 
CAATTCTTAG AGAAAAACAA CGTTGAGTTA ATTGCTAGAG CTCATCAGTT AGTGATGGAA 
GGTTACAAAG AAATGTTCGA CGGTGGATTA GTGACAGTCT GGTCGGCACC GAATTACTGT 
TATCGTTGTG GTAATGTAGC AGCTGTATTG AAGATAGACG ACGATTTGAA TCGTGAATAT 
ACAATTTTTG AAGCTGTTCA GGCACAAAAT GAAGTCGGAA ATGCTATAAT TCCAACCAAA 
AAATCTCAAA TGGACTATTT CTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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PPH3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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