S100a9 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	S100a9
Entrez Gene ID	94195
Full Name	S100 calcium binding protein A9
Synonyms	Mrp14
General protein information	Preferred Names S100 calcium binding protein A9 Names protein S100-A9 MRP-14 S100 calcium binding protein A9 (calgranulin B) calgranulin-B intracellular calcium-binding protein (MRP14) migration inhibitory factor-related protein 14 myeloid-related protein 14 p14
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34
Summary	calcium binding protein that may be associated with acute inflammatory processes [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053587.1	NP_446039.1	protein S100-A9
XM_006232780.2	XP_006232842.1	protein S100-A9 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	S100A9	XP_547585.4
Pan troglodytes (chimpanzee)	S100A9	XP_003308531.1
Rattus norvegicus (Norway rat)	S100a9	NP_446039.1
Homo sapiens (human)	S100A9	NP_002956.1
Bos taurus (cattle)	S100A9	NP_001039793.1
Gallus gallus (chicken)	S100A9	XP_424012.4
Mus musculus (house mouse)	S100a9	NP_001268781.1
	S100A9	XP_001110408.2

Gene Ontology
Bibliography

Related articles in PubMed

Upregulation of calprotectin in mild IgE-mediated ovalbumin hypersensitivity.
Zhu Q, Li F, Wang J, Ma J, Sheng X
Oncotarget8(23)37342-37354(2017 Jun)

Enzyme-linked immunosorbent assay for S100A9 in the stool of rats with dextran sulfate sodium-induced colitis.
Sekiya S, Murata M, Arai S, Murayama H, Kawasaki A, Ashida N, Okada K, Ikemoto M
Journal of immunological methods43944-49(2016 Dec)

CD68 on rat macrophages binds tightly to S100A8 and S100A9 and helps to regulate the cells' immune functions.
Okada K, Arai S, Itoh H, Adachi S, Hayashida M, Nakase H, Ikemoto M
Journal of leukocyte biology100(5)1093-1104(2016 11)

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Barallobre-Barreiro J, Oklu R, Lynch M, Fava M, Baig F, Yin X, Barwari T, Potier DN, Albadawi H, Jahangiri M, Porter KE, Watkins MT, Misra S, Stoughton J, Mayr M
Cardiovascular research110(3)419-30(2016 06)

Decreased S100A9 Expression Promoted Rat Airway Smooth Muscle Cell Proliferation by Stimulating ROS Generation and Inhibiting p38 MAPK.
Yin LM, Han XJ, Duan TT, Xu YD, Wang Y, Ulloa L, Yang YQ
Canadian respiratory journal20161462563(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In dextran sulfate sodium-induced colitis fecal levels of S100A9 detected by ELISA were significantly elevated before microscopic damage was detected.
Title: Enzyme-linked immunosorbent assay for S100A9 in the stool of rats with dextran sulfate sodium-induced colitis.

Recombinant rat r-S100A9 bound to r-CD68. The expression levels of S100A9 were significantly suppressed after the macrophages had been treated with an anti-CD68 antibody. Small interfering CD68 also significantly suppressed activation of macrophages through an autocrine pathway by r-S100A9.
Title: CD68 on rat macrophages binds tightly to S100A8 and S100A9 and helps to regulate the cells' immune functions.

Decreased S100A9 expression promoted airway smooth muscle cell proliferation by stimulating reactive oxygen species generation and inhibiting p38 MAPK.
Title: Decreased S100A9 Expression Promoted Rat Airway Smooth Muscle Cell Proliferation by Stimulating ROS Generation and Inhibiting p38 MAPK.

Expression of S100A9 mRNA and the corresponding protein in cultured NRVCs was similar between control cells and NE-stimulated cells, but was markedly upregulated at 12 hours after the withdrawal of NE
Title: Myocardial Hypertrophic Preconditioning Attenuates Cardiomyocyte Hypertrophy and Slows Progression to Heart Failure Through Upregulation of S100A8/A9.

in ulcerative colitis rats the immune functions of macrophages are regulated in a complex manner by r-S100A8 and/or r-S100A9 through undefined autocrine pathways on the cells
Title: Autocrine pathways involving S100A8 and/or S100A9 that are postulated to regulate the immunological functions of macrophages in rats.

The following S100a9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the S100a9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09977	NM_053587.1 Latest version!	Rattus norvegicus S100 calcium binding protein A9 (S100a9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa24972	XM_006232780.2 Latest version!	Rattus norvegicus S100 calcium binding protein A9 (S100a9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

S100a9 ( NM_053587.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446039.1 Rattus norvegicus S100 calcium binding protein A9 (S100a9), mRNA.

Price & Availability↑

CloneID	ORa09977
Clone ID Related Accession (Same CDS sequence)	NM_053587.1
Accession Version	NM_053587.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Rattus norvegicus(Norway rat)
Product	protein S100-A9
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L18948.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L18948.1, CB611837.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906354, SAMN01906356 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGCCA AAACAGGATC TCAGCTGGAG CGCAGCATAA GCACCATCAT CAATGTTTTC 
CATCAGTACT CTAGGAAGTA TGGACATCCT GACACCCTGA ACAAGGCGGA ATTCAAAGAA 
ATGGTGAATA AGGACTTGCC AAATTTTCTG AAGAGGGAGA AAAGAAATGA AAATCTCCTA 
AGAGACATCA TGGAGGACCT GGACACAAAC CAGGACAATC AACTGTCCTT TGAGGAGTGT 
ATGATGCTGA TGGGAAAGTT GATCTTTGCC TGTCATGAGA AGCTGCATGA GAACAACCCA 
CGTGGGCATG ACCACAGGCA CGGCAAAGGC TGTGGGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446039.1
CDS	32..373
Translation	MAAKTGSQLERSISTIINVFHQYSRKYGHPDTLNKAEFKEMVNKDLPNFLKREKRNENLLRDIMEDLDTNQDNQLSFEECMMLMGKLIFACHEKLHENNPRGHDHRHGKGCGK

Target ORF information:

RefSeq Version	NM_053587.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus S100 calcium binding protein A9 (S100a9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053587.1

ORF Insert Sequence:

ATGGCTGCCA AAACAGGATC TCAGCTGGAG CGCAGCATAA GCACCATCAT CAATGTTTTC 
CATCAGTACT CTAGGAAGTA TGGACATCCT GACACCCTGA ACAAGGCGGA ATTCAAAGAA 
ATGGTGAATA AGGACTTGCC AAATTTTCTG AAGAGGGAGA AAAGAAATGA AAATCTCCTA 
AGAGACATCA TGGAGGACCT GGACACAAAC CAGGACAATC AACTGTCCTT TGAGGAGTGT 
ATGATGCTGA TGGGAAAGTT GATCTTTGCC TGTCATGAGA AGCTGCATGA GAACAACCCA 
CGTGGGCATG ACCACAGGCA CGGCAAAGGC TGTGGGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

S100a9 ( XM_006232780.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006232842.1 Rattus norvegicus S100 calcium binding protein A9 (S100a9), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa24972
Clone ID Related Accession (Same CDS sequence)	XM_006232780.2
Accession Version	XM_006232780.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein S100-A9 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006232780.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGCT TACACTGCTC TTGCCAGACG CTCTGTGGCT CCTTAGCTTT GAGCAAGAAG 
ATGGCTGCCA AAACAGGATC TCAGCTGGAG CGCAGCATAA GCACCATCAT CAATGTTTTC 
CATCAGTACT CTAGGAAGTA TGGACATCCT GACACCCTGA ACAAGGCGGA ATTCAAAGAA 
ATGGTGAATA AGGACTTGCC AAATTTTCTG AAGAGGGAGA AAAGAAATGA AAATCTCCTA 
AGAGACATCA TGGAGGACCT GGACACAAAC CAGGACAATC AACTGTCCTT TGAGGAGTGT 
ATGATGCTGA TGGGAAAGTT GATCTTTGCC TGTCATGAGA AGCTGCATGA GAACAACCCA 
CGTGGGCATG ACCACAGCCA CGGCAAAGGC TGTGGGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006232842.1
CDS	54..455
Translation	MLGLHCSCQTLCGSLALSKKMAAKTGSQLERSISTIINVFHQYSRKYGHPDTLNKAEFKEMVNKDLPNFLKREKRNENLLRDIMEDLDTNQDNQLSFEECMMLMGKLIFACHEKLHENNPRGHDHSHGKGCGK

Target ORF information:

RefSeq Version	XM_006232780.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus S100 calcium binding protein A9 (S100a9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232780.2

ORF Insert Sequence:

ATGCTGGGCT TACACTGCTC TTGCCAGACG CTCTGTGGCT CCTTAGCTTT GAGCAAGAAG 
ATGGCTGCCA AAACAGGATC TCAGCTGGAG CGCAGCATAA GCACCATCAT CAATGTTTTC 
CATCAGTACT CTAGGAAGTA TGGACATCCT GACACCCTGA ACAAGGCGGA ATTCAAAGAA 
ATGGTGAATA AGGACTTGCC AAATTTTCTG AAGAGGGAGA AAAGAAATGA AAATCTCCTA 
AGAGACATCA TGGAGGACCT GGACACAAAC CAGGACAATC AACTGTCCTT TGAGGAGTGT 
ATGATGCTGA TGGGAAAGTT GATCTTTGCC TGTCATGAGA AGCTGCATGA GAACAACCCA 
CGTGGGCATG ACCACAGCCA CGGCAAAGGC TGTGGGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Upregulation of calprotectin in mild IgE-mediated ovalbumin hypersensitivity.
Oncotarget8(23)37342-37354(2017 Jun)
Zhu Q,Li F,Wang J,Ma J,Sheng X

Enzyme-linked immunosorbent assay for S100A9 in the stool of rats with dextran sulfate sodium-induced colitis.
Journal of immunological methods43944-49(2016 Dec)
Sekiya S,Murata M,Arai S,Murayama H,Kawasaki A,Ashida N,Okada K,Ikemoto M

CD68 on rat macrophages binds tightly to S100A8 and S100A9 and helps to regulate the cells' immune functions.
Journal of leukocyte biology100(5)1093-1104(2016 11)
Okada K,Arai S,Itoh H,Adachi S,Hayashida M,Nakase H,Ikemoto M

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Cardiovascular research110(3)419-30(2016 06)
Barallobre-Barreiro J,Oklu R,Lynch M,Fava M,Baig F,Yin X,Barwari T,Potier DN,Albadawi H,Jahangiri M,Porter KE,Watkins MT,Misra S,Stoughton J,Mayr M

Decreased S100A9 Expression Promoted Rat Airway Smooth Muscle Cell Proliferation by Stimulating ROS Generation and Inhibiting p38 MAPK.
Canadian respiratory journal20161462563(2016)
Yin LM,Han XJ,Duan TT,Xu YD,Wang Y,Ulloa L,Yang YQ