Tek cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tek
Entrez Gene ID	89804
Full Name	TEK receptor tyrosine kinase
Synonyms	Tie-2,Tie2
General protein information	Preferred Names TEK receptor tyrosine kinase Names angiopoietin-1 receptor TEK tyrosine kinase, endothelial endothelial-specific receptor tyrosine kinase tyrosine kinases that contain immunoglobulin-like loops and epidermal growth factor-similar domains 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q33
Summary	mediates development of embryonic vasculature; plays a role in tumors and skin wounds [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001105737.1	NP_001099207.1	angiopoietin-1 receptor
XM_008763910.1	XP_008762132.1	angiopoietin-1 receptor isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TEK	NP_000450.2
Mus musculus (house mouse)	Tek	NP_038718.2
Xenopus tropicalis (tropical clawed frog)	tek	XP_002934031.2
	TEK	XP_001105270.1
Rattus norvegicus (Norway rat)	Tek	NP_001099207.1
Pan troglodytes (chimpanzee)	TEK	XP_520519.3
Canis lupus familiaris (dog)	TEK	XP_005626753.1
Bos taurus (cattle)	TEK	NP_776389.1
Gallus gallus (chicken)	TEK	XP_004949637.1
Danio rerio (zebrafish)	tek	NP_571536.1

Gene Ontology
Bibliography

Related articles in PubMed

MicroRNA-150 regulates blood-brain barrier permeability via Tie-2 after permanent middle cerebral artery occlusion in rats.
Fang Z, He QW, Li Q, Chen XL, Baral S, Jin HJ, Zhu YY, Li M, Xia YP, Mao L, Hu B
FASEB journal : official publication of the Federation of American Societies for Experimental Biology30(6)2097-107(2016 06)

Compound 49b Restores Retinal Thickness and Reduces Degenerate Capillaries in the Rat Retina following Ischemia/Reperfusion.
Liu L, Jiang Y, Steinle JJ
PloS one11(7)e0159532(2016)

Temporal expression in rats of receptor tyrosine kinase Tie2 during early wound healing after tooth extraction.
Sato H, Kasai S, Maesawa C
Journal of oral science57(4)313-8(2015)

COMP-angiopoietin 1 increases proliferation, differentiation, and migration of stem-like cells through Tie-2-mediated activation of p38 MAPK and PI3K/Akt signal transduction pathways.
Kook SH, Lim SS, Cho ES, Lee YH, Han SK, Lee KY, Kwon J, Hwang JW, Bae CH, Seo YK, Lee JC
Biochemical and biophysical research communications455(3-4)371-7(2014 Dec)

Tie2-dependent neovascularization of the ischemic hindlimb is mediated by angiopoietin-2.
Lekas M, Lekas P, Mei SH, Deng Y, Dumont DJ, Stewart DJ
PloS one7(9)e43568(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings suggested that miR-150 could regulate claudin-5 expression and endothelial cell survival by targeting Tie-2, thus affecting the permeability of BBB after permanent middle cerebral artery occlusion in rats, and that miR-150 might be a potential alternative target for the treatment of stroke.
Title: MicroRNA-150 regulates blood-brain barrier permeability via Tie-2 after permanent middle cerebral artery occlusion in rats.

Hypoxia significantly increased protein levels of VEGF, KDR, Angiopoiein 1, and Tie2, which were reduced following Compound 49b treatment. These data strongly suggested that Compound 49b protected the retina against I/R-induced injury. This provides additional support for a role of beta-adrenergic receptor actions in the retina.
Title: Compound 49b Restores Retinal Thickness and Reduces Degenerate Capillaries in the Rat Retina following Ischemia/Reperfusion.

Tie2 extracellular domain initially inhibited angiogenesis, and its degradation relieved inhibition of new vessel formation
Title: Temporal expression in rats of receptor tyrosine kinase Tie2 during early wound healing after tooth extraction.

COMP-Ang1-stimulated proliferation, differentiation, and migration of progenitor cells may involve the Tie-2-mediated activation of p38 MAPK and PI3K/Akt pathways
Title: COMP-angiopoietin 1 increases proliferation, differentiation, and migration of stem-like cells through Tie-2-mediated activation of p38 MAPK and PI3K/Akt signal transduction pathways.

Tie2-dependent neovascularization of the ischemic hindlimb is mediated by angiopoietin-2.
Title: Tie2-dependent neovascularization of the ischemic hindlimb is mediated by angiopoietin-2.

The following Tek gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tek cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13904	NM_001105737.1 Latest version!	Rattus norvegicus TEK receptor tyrosine kinase (Tek), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$825.30 $1179.00
ORa43883	XM_008763910.1 Latest version!	Rattus norvegicus TEK receptor tyrosine kinase (Tek), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tek ( NM_001105737.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099207.1 Rattus norvegicus TEK receptor tyrosine kinase (Tek), mRNA.

Price & Availability↑

CloneID	ORa13904
Clone ID Related Accession (Same CDS sequence)	NM_001105737.1
Accession Version	NM_001105737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Rattus norvegicus(Norway rat)
Product	angiopoietin-1 receptor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473998.1. On or before Oct 3, 2007 this sequence version replaced XM_342863.3, XM_001054732.1, XM_001054185.1, XM_001054314.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGACTCTT TAGCCGGCTT AGTTCTCTGT GGAGTCAGCT TGCTCCTTTA TGGACTAGTA 
GAAGGAGCCA TGGACCTGAT CTTGATCAAT TCCCTACCTC TTGTGTCTGA TGCCGAAACA 
TCCCTCACCT GCATTGCTTC TGGGTGGCAC CCCCACGAGC CCATCACCAT AGGAAGGGAC 
TTTGAAGCCT TAATGAACCA GCACCAAGAT CCACTGGAAG TTACTCAAGA TGTGACCAGA 
GAATGGGCGA AAAAGGTTGT TTGGAAGAGA GAAAAGGCCA GTAAGATTAA TGGTGCTTAT 
TTCTGTGAAG GTCGAGTTCG AGGACAGGCT ATAAGGATAC GAACCATGAA GATGCGTCAA 
CAAGCTTCCT TTCTACCTGC TACTCTAACT ATGACCGTGG ACAGGGGAGA TAATGTGAAC 
ATATCCTTCA AAAAGGTGTT AATTAAAGAA GAAGATGCAG TGATTTACAA AAATGGTTCC 
TTCATTCATT CAGTGCCCCG GCATGAAGTT CCTGATATTT TAGAAGTGCA TCTGCCTCAC 
GCTCAGCCCC AGGATGCTGG TGTGTACTCG GCCAGGTACA TAGGAGGAAA CCTGTTCACC 
TCGGCCTTCA CCAGGCTGAT TGTCCGGAGA TGTGAAGCTC AGAAGTGGGG ACCCGACTGT 
AACCGTCCTT GTACTACTTG CAAGAACAAT GGTGTCTGCC ATGAAGATAC TGGGGAGTGC 
ATTTGCCCTC CTGGGTTTAT GGGGAGGACA TGTGAAAAGG CTTGTGAGCC ACACACATTT 
GGCAGGACCT GTAAGGAACG GTGTAGTGGA TCAGAGGGAT GCAAGTCATA TGTGTTCTGT 
CTGCCAGACC CCTATGGGTG TTCCTGTGCC ACAGGCTGGA GGGGGCTGCA GTGCAATGAA 
GCATGTCCAT ACGGTCACTA TGGGCCAGAC TGTAAGCTCA GGTGCCACTG TACCAATGAA 
GAGATGTGTG ACCGCTTCCA AGGATGCCTC TGCTCTCAAG GATGGCAAGG GCTGCAGTGT 
GAGAAAGAAG GCAGGCCAAG GATGACTCCA CAGATAGAGG ATTTGCCAGA TCACATAGAA 
GTAAACAGTG GGAAATTTAA CCCGATCTGC AAGGCCTCTG GCTGGCCACT ACCTACTAGT 
GAAGAAATGA CCCTGGTGAA GCCGGATGGG ACAGTGCTCC AACCAAATGA CTTCAACCAT 
ACAGATCATT TCTCAGTGGC CATATTCACT GTCAACCGGA TCTTACCTCC TGACTCAGGA 
GTTTGGGTCT GCAGTGTGAA CACAGTGGCT GGGATGGTGG AAAAGCCTTT CAACATTTCT 
GTCAAAGTTC TTCCAGAGCC CCTGCATGCC CCAAATGTTA TTGACACTGG ACACAACTTT 
GCTATCATCA ACATCAGCTC TGAGCCTTAC TTTGGGGATG GACCGATCAA ATCCAAGAAG 
CTCTTCTATA AACCTGTCAA TCAGGCTTGG AAATACATTC AAGTGATGAA TGAGATTGTC 
ACACTCAACT ACCTGGAGCC TCGGACTGAC TACGAGCTGT GTGTACAGCT GGTCCGTCCT 
GGAGAGGGTG GAGAAGGACA TCCTGGACCT GTGAGAAGAT TCACAACAGC GTCTATCGGA 
CTCCCTCCTC CAAGAGGTCT CAGTCTCCTA CCCAAAAGCC AGACAGCTCT GAATTTGACT 
TGGCAACCGA TATTTACAAG CTCAGAAGAT GAATTTTATG TGGAAGTTGA GAGGTGGTCC 
CAGCAAACAA GAAGTGATCA GCAGAACATC AAAGTGCCTG GGAACCTGAC TTCCGTGCTG 
CTGAACAACT TACTCCCCAG GGAGCAGTAC AGCGTCCGAG CTAGAGTCAA CACCAAGGCC 
CAGGGGGAGT GGAGTGAAGA ACTCAGGGCC TGGACCCTTA GTGACATTCT CCCTCCTCAG 
CCAGAAAACA TCAAGATCAC CAACATCACT GATTACACAG CTCTGGTTTC TTGGACAATC 
GTGGACGGCT ATTCGATTTC TTCCATCATC ATCCGGTATA AGGTTCAGGG CAAAAATGAA 
GACCAGCACA TTGACGTGAA GATCAAGAAT GCCACCATCA CTCAATACCA GCTCAAGGGC 
CTAGAGCCAG AGACTACATA CCATGTGGAT ATTTTTGCTG AGAACAACAT AGGATCAAGC 
AACCCAGCCT TTTCCCAAGA AATTAGGACA CTTCCAGCCC CTAAAGACCT TGGAGGGGGA 
AAGATGCTAC TTATAGCCAT TCTTGGGTCG GCTGGAATGA CTTGCATCAC CGTGCTATTG 
GCGTTTCTGA TTATGTTGCA ACTGAAGAGA GCAAATGTCC AAAGAAGAAT GGCCCAGGCC 
TTCCAGAACG TGAGAGAAGA ACCAGCTGTT CAGTTCAACT CAGGAACTCT GGCCCTAAAC 
AGGAAGGCCA AAAACAATCC GGATCCCACA ATTTATCCTG TGCTTGACTG GAATGACATC 
AAGTTCCAAG ATGTGATTGG AGAGGGCAAC TTTGGCCAGG TTCTGAAGGC GCGCATCAAG 
AAGGATGGGT TACGGATGGA CGCTGCCATC AAGAGGATGA AAGAATATGC CTCCAAAGAT 
GATCACAGGG ACTTTGCAGG AGAACTGGAG GTTCTTTGTA AACTTGGACA CCATCCGAAC 
ATCATTAATC TCTTGGGAGC ATGTGAACAC AGAGGCTACT TATACCTGGC TATTGAGTAT 
GCCCCACATG GAAACCTCCT GGACTTTCTG CGTAAGAGCC GAGTGCTAGA GACAGACCCT 
GCCTTTGCCA TCGCCAACAG CACGGCTTCC ACACTGTCCT CCCAGCAGCT TCTTCATTTT 
GCTGCAGATG TGGCCCGGGG GATGGACTAC TTGAGCCAAA AACAGTTTAT CCACAGGGAC 
CTGGCTGCCA GAAACATTTT AGTTGGCGAA AACTACATAG CCAAAATAGC AGATTTTGGA 
TTGTCACGAG GTCAAGAAGT GTATGTGAAA AAGACAATGG GAAGGCTCCC AGTGCGCTGG 
ATGGCAATTG AGTCTCTGAA CTATAGTGTC TATACAACCA ACAGTGATGT ATGGTCCTAT 
GGTGTATTGC TCTGGGAGAT CGTTAGCTTA GGAGGCACTC CATACTGCGG CATGACATGT 
GCAGAACTCT ATGAGAAGCT GCCCCAGGGC TACAGATTGG AGAAGCCCCT GAACTGTGAT 
GATGAGGTGT ATGATCTAAT GAGACAATGC TGGAGGGAGA AGCCTTATGA GAGACCATCA 
TTTGCCCAGA TATTGGTGTC CTTAAACAGA ATGCTGGAAG AACGAAAGAC ATACGTGAAC 
ACCACACTTT ATGAGAAGTT TACCTACGCA GGAATTGACT GTTCTGCTGA AGAAGCAGCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099207.1
CDS	329..3691
Translation	MDSLAGLVLCGVSLLLYGLVEGAMDLILINSLPLVSDAETSLTCIASGWHPHEPITIGRDFEALMNQHQDPLEVTQDVTREWAKKVVWKREKASKINGAYFCEGRVRGQAIRIRTMKMRQQASFLPATLTMTVDRGDNVNISFKKVLIKEEDAVIYKNGSFIHSVPRHEVPDILEVHLPHAQPQDAGVYSARYIGGNLFTSAFTRLIVRRCEAQKWGPDCNRPCTTCKNNGVCHEDTGECICPPGFMGRTCEKACEPHTFGRTCKERCSGSEGCKSYVFCLPDPYGCSCATGWRGLQCNEACPYGHYGPDCKLRCHCTNEEMCDRFQGCLCSQGWQGLQCEKEGRPRMTPQIEDLPDHIEVNSGKFNPICKASGWPLPTSEEMTLVKPDGTVLQPNDFNHTDHFSVAIFTVNRILPPDSGVWVCSVNTVAGMVEKPFNISVKVLPEPLHAPNVIDTGHNFAIINISSEPYFGDGPIKSKKLFYKPVNQAWKYIQVMNEIVTLNYLEPRTDYELCVQLVRPGEGGEGHPGPVRRFTTASIGLPPPRGLSLLPKSQTALNLTWQPIFTSSEDEFYVEVERWSQQTRSDQQNIKVPGNLTSVLLNNLLPREQYSVRARVNTKAQGEWSEELRAWTLSDILPPQPENIKITNITDYTALVSWTIVDGYSISSIIIRYKVQGKNEDQHIDVKIKNATITQYQLKGLEPETTYHVDIFAENNIGSSNPAFSQEIRTLPAPKDLGGGKMLLIAILGSAGMTCITVLLAFLIMLQLKRANVQRRMAQAFQNVREEPAVQFNSGTLALNRKAKNNPDPTIYPVLDWNDIKFQDVIGEGNFGQVLKARIKKDGLRMDAAIKRMKEYASKDDHRDFAGELEVLCKLGHHPNIINLLGACEHRGYLYLAIEYAPHGNLLDFLRKSRVLETDPAFAIANSTASTLSSQQLLHFAADVARGMDYLSQKQFIHRDLAARNILVGENYIAKIADFGLSRGQEVYVKKTMGRLPVRWMAIESLNYSVYTTNSDVWSYGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRLEKPLNCDDEVYDLMRQCWREKPYERPSFAQILVSLNRMLEERKTYVNTTLYEKFTYAGIDCSAEEAA

Target ORF information:

RefSeq Version	NM_001105737.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus TEK receptor tyrosine kinase (Tek), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105737.1

ORF Insert Sequence:

ATGGACTCTT TAGCCGGCTT AGTTCTCTGT GGAGTCAGCT TGCTCCTTTA TGGACTAGTA 
GAAGGAGCCA TGGACCTGAT CTTGATCAAT TCCCTACCTC TTGTGTCTGA TGCCGAAACA 
TCCCTCACCT GCATTGCTTC TGGGTGGCAC CCCCACGAGC CCATCACCAT AGGAAGGGAC 
TTTGAAGCCT TAATGAACCA GCACCAAGAT CCACTGGAAG TTACTCAAGA TGTGACCAGA 
GAATGGGCGA AAAAGGTTGT TTGGAAGAGA GAAAAGGCCA GTAAGATTAA TGGTGCTTAT 
TTCTGTGAAG GTCGAGTTCG AGGACAGGCT ATAAGGATAC GAACCATGAA GATGCGTCAA 
CAAGCTTCCT TTCTACCTGC TACTCTAACT ATGACCGTGG ACAGGGGAGA TAATGTGAAC 
ATATCCTTCA AAAAGGTGTT AATTAAAGAA GAAGATGCAG TGATTTACAA AAATGGTTCC 
TTCATTCATT CAGTGCCCCG GCATGAAGTT CCTGATATTT TAGAAGTGCA TCTGCCTCAC 
GCTCAGCCCC AGGATGCTGG TGTGTACTCG GCCAGGTACA TAGGAGGAAA CCTGTTCACC 
TCGGCCTTCA CCAGGCTGAT TGTCCGGAGA TGTGAAGCTC AGAAGTGGGG ACCCGACTGT 
AACCGTCCTT GTACTACTTG CAAGAACAAT GGTGTCTGCC ATGAAGATAC TGGGGAGTGC 
ATTTGCCCTC CTGGGTTTAT GGGGAGGACA TGTGAAAAGG CTTGTGAGCC ACACACATTT 
GGCAGGACCT GTAAGGAACG GTGTAGTGGA TCAGAGGGAT GCAAGTCATA TGTGTTCTGT 
CTGCCAGACC CCTATGGGTG TTCCTGTGCC ACAGGCTGGA GGGGGCTGCA GTGCAATGAA 
GCATGTCCAT ACGGTCACTA TGGGCCAGAC TGTAAGCTCA GGTGCCACTG TACCAATGAA 
GAGATGTGTG ACCGCTTCCA AGGATGCCTC TGCTCTCAAG GATGGCAAGG GCTGCAGTGT 
GAGAAAGAAG GCAGGCCAAG GATGACTCCA CAGATAGAGG ATTTGCCAGA TCACATAGAA 
GTAAACAGTG GGAAATTTAA CCCGATCTGC AAGGCCTCTG GCTGGCCACT ACCTACTAGT 
GAAGAAATGA CCCTGGTGAA GCCGGATGGG ACAGTGCTCC AACCAAATGA CTTCAACCAT 
ACAGATCATT TCTCAGTGGC CATATTCACT GTCAACCGGA TCTTACCTCC TGACTCAGGA 
GTTTGGGTCT GCAGTGTGAA CACAGTGGCT GGGATGGTGG AAAAGCCTTT CAACATTTCT 
GTCAAAGTTC TTCCAGAGCC CCTGCATGCC CCAAATGTTA TTGACACTGG ACACAACTTT 
GCTATCATCA ACATCAGCTC TGAGCCTTAC TTTGGGGATG GACCGATCAA ATCCAAGAAG 
CTCTTCTATA AACCTGTCAA TCAGGCTTGG AAATACATTC AAGTGATGAA TGAGATTGTC 
ACACTCAACT ACCTGGAGCC TCGGACTGAC TACGAGCTGT GTGTACAGCT GGTCCGTCCT 
GGAGAGGGTG GAGAAGGACA TCCTGGACCT GTGAGAAGAT TCACAACAGC GTCTATCGGA 
CTCCCTCCTC CAAGAGGTCT CAGTCTCCTA CCCAAAAGCC AGACAGCTCT GAATTTGACT 
TGGCAACCGA TATTTACAAG CTCAGAAGAT GAATTTTATG TGGAAGTTGA GAGGTGGTCC 
CAGCAAACAA GAAGTGATCA GCAGAACATC AAAGTGCCTG GGAACCTGAC TTCCGTGCTG 
CTGAACAACT TACTCCCCAG GGAGCAGTAC AGCGTCCGAG CTAGAGTCAA CACCAAGGCC 
CAGGGGGAGT GGAGTGAAGA ACTCAGGGCC TGGACCCTTA GTGACATTCT CCCTCCTCAG 
CCAGAAAACA TCAAGATCAC CAACATCACT GATTACACAG CTCTGGTTTC TTGGACAATC 
GTGGACGGCT ATTCGATTTC TTCCATCATC ATCCGGTATA AGGTTCAGGG CAAAAATGAA 
GACCAGCACA TTGACGTGAA GATCAAGAAT GCCACCATCA CTCAATACCA GCTCAAGGGC 
CTAGAGCCAG AGACTACATA CCATGTGGAT ATTTTTGCTG AGAACAACAT AGGATCAAGC 
AACCCAGCCT TTTCCCAAGA AATTAGGACA CTTCCAGCCC CTAAAGACCT TGGAGGGGGA 
AAGATGCTAC TTATAGCCAT TCTTGGGTCG GCTGGAATGA CTTGCATCAC CGTGCTATTG 
GCGTTTCTGA TTATGTTGCA ACTGAAGAGA GCAAATGTCC AAAGAAGAAT GGCCCAGGCC 
TTCCAGAACG TGAGAGAAGA ACCAGCTGTT CAGTTCAACT CAGGAACTCT GGCCCTAAAC 
AGGAAGGCCA AAAACAATCC GGATCCCACA ATTTATCCTG TGCTTGACTG GAATGACATC 
AAGTTCCAAG ATGTGATTGG AGAGGGCAAC TTTGGCCAGG TTCTGAAGGC GCGCATCAAG 
AAGGATGGGT TACGGATGGA CGCTGCCATC AAGAGGATGA AAGAATATGC CTCCAAAGAT 
GATCACAGGG ACTTTGCAGG AGAACTGGAG GTTCTTTGTA AACTTGGACA CCATCCGAAC 
ATCATTAATC TCTTGGGAGC ATGTGAACAC AGAGGCTACT TATACCTGGC TATTGAGTAT 
GCCCCACATG GAAACCTCCT GGACTTTCTG CGTAAGAGCC GAGTGCTAGA GACAGACCCT 
GCCTTTGCCA TCGCCAACAG CACGGCTTCC ACACTGTCCT CCCAGCAGCT TCTTCATTTT 
GCTGCAGATG TGGCCCGGGG GATGGACTAC TTGAGCCAAA AACAGTTTAT CCACAGGGAC 
CTGGCTGCCA GAAACATTTT AGTTGGCGAA AACTACATAG CCAAAATAGC AGATTTTGGA 
TTGTCACGAG GTCAAGAAGT GTATGTGAAA AAGACAATGG GAAGGCTCCC AGTGCGCTGG 
ATGGCAATTG AGTCTCTGAA CTATAGTGTC TATACAACCA ACAGTGATGT ATGGTCCTAT 
GGTGTATTGC TCTGGGAGAT CGTTAGCTTA GGAGGCACTC CATACTGCGG CATGACATGT 
GCAGAACTCT ATGAGAAGCT GCCCCAGGGC TACAGATTGG AGAAGCCCCT GAACTGTGAT 
GATGAGGTGT ATGATCTAAT GAGACAATGC TGGAGGGAGA AGCCTTATGA GAGACCATCA 
TTTGCCCAGA TATTGGTGTC CTTAAACAGA ATGCTGGAAG AACGAAAGAC ATACGTGAAC 
ACCACACTTT ATGAGAAGTT TACCTACGCA GGAATTGACT GTTCTGCTGA AGAAGCAGCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tek ( XM_008763910.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762132.1 Rattus norvegicus TEK receptor tyrosine kinase (Tek), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43883
Clone ID Related Accession (Same CDS sequence)	XM_008763910.1
Accession Version	XM_008763910.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	angiopoietin-1 receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCCTC CTGGGTTGCC ATGGCCTCTC CCAGGACCGA GACTAGTAGA AGGAGCCATG 
GACCTGATCT TGATCAATTC CCTACCTCTT GTGTCTGATG CCGAAACATC CCTCACCTGC 
ATTGCTTCTG GGTGGCACCC CCACGAGCCC ATCACCATAG GAAGGGACTT TGAAGCCTTA 
ATGAACCAGC ACCAAGATCC ACTGGAAGTT ACTCAAGATG TGACCAGAGA ATGGGCGAAA 
AAGGTTGTTT GGAAGAGAGA AAAGGCCAGT AAGATTAATG GTGCTTATTT CTGTGAAGGT 
CGAGTTCGAG GACAGGCTAT AAGGATACGA ACCATGAAGA TGCGTCAACA AGCTTCCTTT 
CTACCTGCTA CTCTAACTAT GACCGTGGAC AGGGGAGATA ATGTGAACAT ATCCTTCAAA 
AAGGTGTTAA TTAAAGAAGA AGATGCAGTG ATTTACAAAA ATGGTTCCTT CATTCATTCA 
GTGCCCCGGC ATGAAGTTCC TGATATTTTA GAAGTGCATC TGCCTCACGC TCAGCCCCAG 
GATGCTGGTG TGTACTCGGC CAGGTACATA GGAGGAAACC TGTTCACCTC GGCCTTCACC 
AGGCTGATTG TCCGGAGATG TGAAGCTCAG AAGTGGGGAC CCGACTGTAA CCGTCCTTGT 
ACTACTTGCA AGAACAATGG TGTCTGCCAT GAAGATACTG GGGAGTGCAT TTGCCCTCCT 
GGGTTTATGG GGAGGACATG TGAAAAGGCT TGTGAGCCAC ACACATTTGG CAGGACCTGT 
AAGGAACGGT GTAGTGGATC AGAGGGATGC AAGTCATATG TGTTCTGTCT GCCAGACCCC 
TATGGGTGTT CCTGTGCCAC AGGCTGGAGG GGGCTGCAGT GCAATGAAGC ATGTCCATAC 
GGTCACTATG GGCCAGACTG TAAGCTCAGG TGCCACTGTA CCAATGAAGA GATGTGTGAC 
CGCTTCCAAG GATGCCTCTG CTCTCAAGGA TGGCAAGGGC TGCAGTGTGA GAAAGAAGGC 
AGGCCAAGGA TGACTCCACA GATAGAGGAT TTGCCAGATC ACATAGAAGT AAACAGTGGG 
AAATTTAACC CGATCTGCAA GGCCTCTGGC TGGCCACTAC CTACTAGTGA AGAAATGACC 
CTGGTGAAGC CGGATGGGAC AGTGCTCCAA CCAAATGACT TCAACCATAC AGATCATTTC 
TCAGTGGCCA TATTCACTGT CAACCGGATC TTACCTCCTG ACTCAGGAGT TTGGGTCTGC 
AGTGTGAACA CAGTGGCTGG GATGGTGGAA AAGCCTTTCA ACATTTCTGT CAAAGTTCTT 
CCAGAGCCCC TGCATGCCCC AAATGTTATT GACACTGGAC ACAACTTTGC TATCATCAAC 
ATCAGCTCTG AGCCTTACTT TGGGGATGGA CCGATCAAAT CCAAGAAGCT CTTCTATAAA 
CCTGTCAATC AGGCTTGGAA ATACATTCAA GTGATGAATG AGATTGTCAC ACTCAACTAC 
CTGGAGCCTC GGACTGACTA CGAGCTGTGT GTACAGCTGG TCCGTCCTGG AGAGGGTGGA 
GAAGGACATC CTGGACCTGT GAGAAGATTC ACAACAGCGT CTATCGGACT CCCTCCTCCA 
AGAGGTCTCA GTCTCCTACC CAAAAGCCAG ACAGCTCTGA ATTTGACTTG GCAACCGATA 
TTTACAAGCT CAGAAGATGA ATTTTATGTG GAAGTTGAGA GGTGGTCCCA GCAAACAAGA 
AGTGATCAGC AGAACATCAA AGTGCCTGGG AACCTGACTT CCGTGCTGCT GAACAACTTA 
CTCCCCAGGG AGCAGTACAG CGTCCGAGCT AGAGTCAACA CCAAGGCCCA GGGGGAGTGG 
AGTGAAGAAC TCAGGGCCTG GACCCTTAGT GACATTCTCC CTCCTCAGCC AGAAAACATC 
AAGATCACCA ACATCACTGA TTACACAGCT CTGGTTTCTT GGACAATCGT GGACGGCTAT 
TCGATTTCTT CCATCATCAT CCGGTATAAG GTTCAGGGCA AAAATGAAGA CCAGCACATT 
GACGTGAAGA TCAAGAATGC CACCATCACT CAATACCAGC TCAAGGGCCT AGAGCCAGAG 
ACTACATACC ATGTGGATAT TTTTGCTGAG AACAACATAG GATCAAGCAA CCCAGCCTTT 
TCCCAAGAAA TTAGGACACT TCCAGCCCCT AAAGACCTTG GAGGGGGAAA GATGCTACTT 
ATAGCCATTC TTGGGTCGGC TGGAATGACT TGCATCACCG TGCTATTGGC GTTTCTGATT 
ATGTTGCAAC TGAAGAGAGC AAATGTCCAA AGAAGAATGG CCCAGGCCTT CCAGAACGTG 
AGAGAAGAAC CAGCTGTTCA GTTCAACTCA GGAACTCTGG CCCTAAACAG GAAGGCCAAA 
AACAATCCGG ATCCCACAAT TTATCCTGTG CTTGACTGGA ATGACATCAA GTTCCAAGAT 
GTGATTGGAG AGGGCAACTT TGGCCAGGTT CTGAAGGCGC GCATCAAGAA GGATGGGTTA 
CGGATGGACG CTGCCATCAA GAGGATGAAA GAATATGCCT CCAAAGATGA TCACAGGGAC 
TTTGCAGGAG AACTGGAGGT TCTTTGTAAA CTTGGACACC ATCCGAACAT CATTAATCTC 
TTGGGAGCAT GTGAACACAG AGGCTACTTA TACCTGGCTA TTGAGTATGC CCCACATGGA 
AACCTCCTGG ACTTTCTGCG TAAGAGCCGA GTGCTAGAGA CAGACCCTGC CTTTGCCATC 
GCCAACAGCA CGGCTTCCAC ACTGTCCTCC CAGCAGCTTC TTCATTTTGC TGCAGATGTG 
GCCCGGGGGA TGGACTACTT GAGCCAAAAA CAGTTTATCC ACAGGGACCT GGCTGCCAGA 
AACATTTTAG TTGGCGAAAA CTACATAGCC AAAATAGCAG ATTTTGGATT GTCACGAGGT 
CAAGAAGTGT ATGTGAAAAA GACAATGGGA AGGCTCCCAG TGCGCTGGAT GGCAATTGAG 
TCTCTGAACT ATAGTGTCTA TACAACCAAC AGTGATGTAT GGTCCTATGG TGTATTGCTC 
TGGGAGATCG TTAGCTTAGG AGGCACTCCA TACTGCGGCA TGACATGTGC AGAACTCTAT 
GAGAAGCTGC CCCAGGGCTA CAGATTGGAG AAGCCCCTGA ACTGTGATGA TGAGGTGTAT 
GATCTAATGA GACAATGCTG GAGGGAGAAG CCTTATGAGA GACCATCATT TGCCCAGATA 
TTGGTGTCCT TAAACAGAAT GCTGGAAGAA CGAAAGACAT ACGTGAACAC CACACTTTAT 
GAGAAGTTTA CCTACGCAGG AATTGACTGT TCTGCTGAAG AAGCAGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762132.1
CDS	161..3511
Translation	MLPPGLPWPLPGPRLVEGAMDLILINSLPLVSDAETSLTCIASGWHPHEPITIGRDFEALMNQHQDPLEVTQDVTREWAKKVVWKREKASKINGAYFCEGRVRGQAIRIRTMKMRQQASFLPATLTMTVDRGDNVNISFKKVLIKEEDAVIYKNGSFIHSVPRHEVPDILEVHLPHAQPQDAGVYSARYIGGNLFTSAFTRLIVRRCEAQKWGPDCNRPCTTCKNNGVCHEDTGECICPPGFMGRTCEKACEPHTFGRTCKERCSGSEGCKSYVFCLPDPYGCSCATGWRGLQCNEACPYGHYGPDCKLRCHCTNEEMCDRFQGCLCSQGWQGLQCEKEGRPRMTPQIEDLPDHIEVNSGKFNPICKASGWPLPTSEEMTLVKPDGTVLQPNDFNHTDHFSVAIFTVNRILPPDSGVWVCSVNTVAGMVEKPFNISVKVLPEPLHAPNVIDTGHNFAIINISSEPYFGDGPIKSKKLFYKPVNQAWKYIQVMNEIVTLNYLEPRTDYELCVQLVRPGEGGEGHPGPVRRFTTASIGLPPPRGLSLLPKSQTALNLTWQPIFTSSEDEFYVEVERWSQQTRSDQQNIKVPGNLTSVLLNNLLPREQYSVRARVNTKAQGEWSEELRAWTLSDILPPQPENIKITNITDYTALVSWTIVDGYSISSIIIRYKVQGKNEDQHIDVKIKNATITQYQLKGLEPETTYHVDIFAENNIGSSNPAFSQEIRTLPAPKDLGGGKMLLIAILGSAGMTCITVLLAFLIMLQLKRANVQRRMAQAFQNVREEPAVQFNSGTLALNRKAKNNPDPTIYPVLDWNDIKFQDVIGEGNFGQVLKARIKKDGLRMDAAIKRMKEYASKDDHRDFAGELEVLCKLGHHPNIINLLGACEHRGYLYLAIEYAPHGNLLDFLRKSRVLETDPAFAIANSTASTLSSQQLLHFAADVARGMDYLSQKQFIHRDLAARNILVGENYIAKIADFGLSRGQEVYVKKTMGRLPVRWMAIESLNYSVYTTNSDVWSYGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRLEKPLNCDDEVYDLMRQCWREKPYERPSFAQILVSLNRMLEERKTYVNTTLYEKFTYAGIDCSAEEAA

Target ORF information:

RefSeq Version	XM_008763910.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus TEK receptor tyrosine kinase (Tek), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763910.1

ORF Insert Sequence:

ATGCTGCCTC CTGGGTTGCC ATGGCCTCTC CCAGGACCGA GACTAGTAGA AGGAGCCATG 
GACCTGATCT TGATCAATTC CCTACCTCTT GTGTCTGATG CCGAAACATC CCTCACCTGC 
ATTGCTTCTG GGTGGCACCC CCACGAGCCC ATCACCATAG GAAGGGACTT TGAAGCCTTA 
ATGAACCAGC ACCAAGATCC ACTGGAAGTT ACTCAAGATG TGACCAGAGA ATGGGCGAAA 
AAGGTTGTTT GGAAGAGAGA AAAGGCCAGT AAGATTAATG GTGCTTATTT CTGTGAAGGT 
CGAGTTCGAG GACAGGCTAT AAGGATACGA ACCATGAAGA TGCGTCAACA AGCTTCCTTT 
CTACCTGCTA CTCTAACTAT GACCGTGGAC AGGGGAGATA ATGTGAACAT ATCCTTCAAA 
AAGGTGTTAA TTAAAGAAGA AGATGCAGTG ATTTACAAAA ATGGTTCCTT CATTCATTCA 
GTGCCCCGGC ATGAAGTTCC TGATATTTTA GAAGTGCATC TGCCTCACGC TCAGCCCCAG 
GATGCTGGTG TGTACTCGGC CAGGTACATA GGAGGAAACC TGTTCACCTC GGCCTTCACC 
AGGCTGATTG TCCGGAGATG TGAAGCTCAG AAGTGGGGAC CCGACTGTAA CCGTCCTTGT 
ACTACTTGCA AGAACAATGG TGTCTGCCAT GAAGATACTG GGGAGTGCAT TTGCCCTCCT 
GGGTTTATGG GGAGGACATG TGAAAAGGCT TGTGAGCCAC ACACATTTGG CAGGACCTGT 
AAGGAACGGT GTAGTGGATC AGAGGGATGC AAGTCATATG TGTTCTGTCT GCCAGACCCC 
TATGGGTGTT CCTGTGCCAC AGGCTGGAGG GGGCTGCAGT GCAATGAAGC ATGTCCATAC 
GGTCACTATG GGCCAGACTG TAAGCTCAGG TGCCACTGTA CCAATGAAGA GATGTGTGAC 
CGCTTCCAAG GATGCCTCTG CTCTCAAGGA TGGCAAGGGC TGCAGTGTGA GAAAGAAGGC 
AGGCCAAGGA TGACTCCACA GATAGAGGAT TTGCCAGATC ACATAGAAGT AAACAGTGGG 
AAATTTAACC CGATCTGCAA GGCCTCTGGC TGGCCACTAC CTACTAGTGA AGAAATGACC 
CTGGTGAAGC CGGATGGGAC AGTGCTCCAA CCAAATGACT TCAACCATAC AGATCATTTC 
TCAGTGGCCA TATTCACTGT CAACCGGATC TTACCTCCTG ACTCAGGAGT TTGGGTCTGC 
AGTGTGAACA CAGTGGCTGG GATGGTGGAA AAGCCTTTCA ACATTTCTGT CAAAGTTCTT 
CCAGAGCCCC TGCATGCCCC AAATGTTATT GACACTGGAC ACAACTTTGC TATCATCAAC 
ATCAGCTCTG AGCCTTACTT TGGGGATGGA CCGATCAAAT CCAAGAAGCT CTTCTATAAA 
CCTGTCAATC AGGCTTGGAA ATACATTCAA GTGATGAATG AGATTGTCAC ACTCAACTAC 
CTGGAGCCTC GGACTGACTA CGAGCTGTGT GTACAGCTGG TCCGTCCTGG AGAGGGTGGA 
GAAGGACATC CTGGACCTGT GAGAAGATTC ACAACAGCGT CTATCGGACT CCCTCCTCCA 
AGAGGTCTCA GTCTCCTACC CAAAAGCCAG ACAGCTCTGA ATTTGACTTG GCAACCGATA 
TTTACAAGCT CAGAAGATGA ATTTTATGTG GAAGTTGAGA GGTGGTCCCA GCAAACAAGA 
AGTGATCAGC AGAACATCAA AGTGCCTGGG AACCTGACTT CCGTGCTGCT GAACAACTTA 
CTCCCCAGGG AGCAGTACAG CGTCCGAGCT AGAGTCAACA CCAAGGCCCA GGGGGAGTGG 
AGTGAAGAAC TCAGGGCCTG GACCCTTAGT GACATTCTCC CTCCTCAGCC AGAAAACATC 
AAGATCACCA ACATCACTGA TTACACAGCT CTGGTTTCTT GGACAATCGT GGACGGCTAT 
TCGATTTCTT CCATCATCAT CCGGTATAAG GTTCAGGGCA AAAATGAAGA CCAGCACATT 
GACGTGAAGA TCAAGAATGC CACCATCACT CAATACCAGC TCAAGGGCCT AGAGCCAGAG 
ACTACATACC ATGTGGATAT TTTTGCTGAG AACAACATAG GATCAAGCAA CCCAGCCTTT 
TCCCAAGAAA TTAGGACACT TCCAGCCCCT AAAGACCTTG GAGGGGGAAA GATGCTACTT 
ATAGCCATTC TTGGGTCGGC TGGAATGACT TGCATCACCG TGCTATTGGC GTTTCTGATT 
ATGTTGCAAC TGAAGAGAGC AAATGTCCAA AGAAGAATGG CCCAGGCCTT CCAGAACGTG 
AGAGAAGAAC CAGCTGTTCA GTTCAACTCA GGAACTCTGG CCCTAAACAG GAAGGCCAAA 
AACAATCCGG ATCCCACAAT TTATCCTGTG CTTGACTGGA ATGACATCAA GTTCCAAGAT 
GTGATTGGAG AGGGCAACTT TGGCCAGGTT CTGAAGGCGC GCATCAAGAA GGATGGGTTA 
CGGATGGACG CTGCCATCAA GAGGATGAAA GAATATGCCT CCAAAGATGA TCACAGGGAC 
TTTGCAGGAG AACTGGAGGT TCTTTGTAAA CTTGGACACC ATCCGAACAT CATTAATCTC 
TTGGGAGCAT GTGAACACAG AGGCTACTTA TACCTGGCTA TTGAGTATGC CCCACATGGA 
AACCTCCTGG ACTTTCTGCG TAAGAGCCGA GTGCTAGAGA CAGACCCTGC CTTTGCCATC 
GCCAACAGCA CGGCTTCCAC ACTGTCCTCC CAGCAGCTTC TTCATTTTGC TGCAGATGTG 
GCCCGGGGGA TGGACTACTT GAGCCAAAAA CAGTTTATCC ACAGGGACCT GGCTGCCAGA 
AACATTTTAG TTGGCGAAAA CTACATAGCC AAAATAGCAG ATTTTGGATT GTCACGAGGT 
CAAGAAGTGT ATGTGAAAAA GACAATGGGA AGGCTCCCAG TGCGCTGGAT GGCAATTGAG 
TCTCTGAACT ATAGTGTCTA TACAACCAAC AGTGATGTAT GGTCCTATGG TGTATTGCTC 
TGGGAGATCG TTAGCTTAGG AGGCACTCCA TACTGCGGCA TGACATGTGC AGAACTCTAT 
GAGAAGCTGC CCCAGGGCTA CAGATTGGAG AAGCCCCTGA ACTGTGATGA TGAGGTGTAT 
GATCTAATGA GACAATGCTG GAGGGAGAAG CCTTATGAGA GACCATCATT TGCCCAGATA 
TTGGTGTCCT TAAACAGAAT GCTGGAAGAA CGAAAGACAT ACGTGAACAC CACACTTTAT 
GAGAAGTTTA CCTACGCAGG AATTGACTGT TCTGCTGAAG AAGCAGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MicroRNA-150 regulates blood-brain barrier permeability via Tie-2 after permanent middle cerebral artery occlusion in rats.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology30(6)2097-107(2016 06)
Fang Z,He QW,Li Q,Chen XL,Baral S,Jin HJ,Zhu YY,Li M,Xia YP,Mao L,Hu B

Compound 49b Restores Retinal Thickness and Reduces Degenerate Capillaries in the Rat Retina following Ischemia/Reperfusion.
PloS one11(7)e0159532(2016)
Liu L,Jiang Y,Steinle JJ

Temporal expression in rats of receptor tyrosine kinase Tie2 during early wound healing after tooth extraction.
Journal of oral science57(4)313-8(2015)
Sato H,Kasai S,Maesawa C

COMP-angiopoietin 1 increases proliferation, differentiation, and migration of stem-like cells through Tie-2-mediated activation of p38 MAPK and PI3K/Akt signal transduction pathways.
Biochemical and biophysical research communications455(3-4)371-7(2014 Dec)
Kook SH,Lim SS,Cho ES,Lee YH,Han SK,Lee KY,Kwon J,Hwang JW,Bae CH,Seo YK,Lee JC

Tie2-dependent neovascularization of the ischemic hindlimb is mediated by angiopoietin-2.
PloS one7(9)e43568(2012)
Lekas M,Lekas P,Mei SH,Deng Y,Dumont DJ,Stewart DJ