Nae1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nae1
Entrez Gene ID	84019
Full Name	NEDD8 activating enzyme E1 subunit 1
Synonyms	Appbp1,RGD1563180
General protein information	Preferred Names NEDD8 activating enzyme E1 subunit 1 Names NEDD8-activating enzyme E1 regulatory subunit APP-binding protein 1 amyloid beta precursor protein-binding protein 1, 59 kDa
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19p14
Summary	may induce apoptosis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_032072.4	NP_114461.3	NEDD8-activating enzyme E1 regulatory subunit

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NAE1	NP_001233317.1
Gallus gallus (chicken)	NAE1	NP_001006129.1
Arabidopsis thaliana (thale cress)	AXL	NP_180800.1
Arabidopsis thaliana (thale cress)	AXR1	NP_172010.1
Homo sapiens (human)	NAE1	NP_001273429.1
	NAE1	XP_001085059.1
Bos taurus (cattle)	NAE1	NP_001179962.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005544	XP_315544.2
Canis lupus familiaris (dog)	NAE1	XP_003639123.1
Rattus norvegicus (Norway rat)	Nae1	NP_114461.2
Danio rerio (zebrafish)	nae1	NP_956793.1
Drosophila melanogaster (fruit fly)	APP-BP1	NP_648435.1
Oryza sativa (rice)	Os03g0820100	NP_001051717.1
Xenopus tropicalis (tropical clawed frog)	nae1	NP_001120050.1
Mus musculus (house mouse)	Nae1	NP_659180.1

Gene Ontology
Bibliography

Related articles in PubMed

Focal transient ischemia increases APP-BP1 expression in neural progenitor cells.
Joo Y, Lee SH, Ha S, Hong BH, Kim JH, Suh YH, Kim HS
Neuroreport22(5)200-5(2011 Mar)

Amyloid precursor protein binding protein-1 modulates cell cycle progression in fetal neural stem cells.
Joo Y, Ha S, Hong BH, Kim Ja, Chang KA, Liew H, Kim S, Sun W, Kim JH, Chong YH, Suh YH, Kim HS
PloS one5(12)e14203(2010 Dec)

TRIP12 functions as an E3 ubiquitin ligase of APP-BP1.
Park Y, Yoon SK, Yoon JB
Biochemical and biophysical research communications374(2)294-8(2008 Sep)

Rab5 mediates an amyloid precursor protein signaling pathway that leads to apoptosis.
Laifenfeld D, Patzek LJ, McPhie DL, Chen Y, Levites Y, Cataldo AM, Neve RL
The Journal of neuroscience : the official journal of the Society for Neuroscience27(27)7141-53(2007 Jul)

APP-BP1 mediates APP-induced apoptosis and DNA synthesis and is increased in Alzheimer's disease brain.
Chen Y, Liu W, McPhie DL, Hassinger L, Neve RL
The Journal of cell biology163(1)27-33(2003 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The upregulation of APP-BP1 expression in neural progenitor cells after focal transient ischemia suggests that this protein contributes to the neurogenesis induced by transient ischemia and reperfusion
Title: Focal transient ischemia increases APP-BP1 expression in neural progenitor cells.

APP-BP1 plays an important role in the cell cycle progression of fetal neural stem cells
Title: Amyloid precursor protein binding protein-1 modulates cell cycle progression in fetal neural stem cells.

The following Nae1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nae1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa43810	NM_032072.4 Latest version!	Rattus norvegicus NEDD8 activating enzyme E1 subunit 1 (Nae1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$314.30 $449.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nae1 ( NM_032072.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_114461.3 Rattus norvegicus NEDD8 activating enzyme E1 subunit 1 (Nae1), mRNA.

Price & Availability↑

CloneID	ORa43810
Clone ID Related Accession (Same CDS sequence)	NM_032072.4
Accession Version	NM_032072.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-16
Organism	Rattus norvegicus(Norway rat)
Product	NEDD8-activating enzyme E1 regulatory subunit
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC127481.1. On Sep 25, 2014 this sequence version replaced NM_032072.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC127481.1, U90829.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCAGC AAGGGAAGAT ACTCAAGGAG CAAAAGTACG ACCGGCAGCT GAGGTTGTGG 
GGTGATCATG GTCAAGAAGC TTTGGAATCT GCTCACGTTT GCTTGATAAA TGCCACAGCT 
ACAGGAACGG AAATTCTTAA AAACTTGGTA CTGCCAGGTA TAGGATCATT TACAATTATC 
GATGGAAATC AGGTCAGCGG AGAAGATGTT GGAAACAATT TTTTCCTTCA AAAATGCAGT 
ATTGGCAAGA ATCGAGCTCA AGCTGCCATG GAATTCTTAC AAGAGCTAAA TAGCGATGTC 
TCTGGAAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGATCC TTCATTTTTC 
TGTAGGTTTA CTATTGTGGT TGCAACTCAG CTTCTTGAAA GTACATTACT ACGTTTAGCA 
GATGTCCTCT GGAATTCCCA GATCCCTCTT TTGATCTGTA GGACATATGG CCTAGTTGGT 
TATATGAGGA TTATTATAAA AGAACATCCA GTAATAGAAT CTCATCCAGA TAATGCATTG 
GAGGACCTAC GACTAGATAA GCCATTTCCT GAACTGAGAG AACATTTTCA GTCTTATGAC 
TTGGACCATA TGGAAAAAAA GGACCATAGC CATACTCCAT GGATTGTGAT CATAGCTAAA 
TATTTAGCCC AGTGGTATAG TGAGACAAAT GGACGAATTC CTAAAAGTTA TAAAGAAAAA 
GAGGACTTCA GAGAATTGAT AAGACAAGGG ATTTTAAAGA ATGAGAATGG AGCTCCAGAA 
GATGAAGAGA ATTTTGAAGA AGCTATTAAA AATGTGAACA CAGCACTAAA TACAACTCAG 
ATCCCAAGCA GTATTGAAGA TATATTTAAT GATGATCGCT GCATAAATAT CACCAAACAG 
ACCCCATCTT TTTGGATTTT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA GGAGGGTCAA 
GGAAATTTAC CTGTTCGAGG CACAATTCCT GATATGATTG CAGATTCAAG CAAATACATA 
AAATTGCAGA ATGTTTATCG TGAGAAAGCA AAGAAAGATG CTGCTGCTGT AGGTAATCAT 
GTTGCCAAAT TGCTTCAGTC TGTTGGCCAG GCACCAGAGT CCATTTCAGA GAAAGAATTA 
AAACTGCTTT GTAGCAATTC TGCATTTCTT CGAGTGGTAA GATGTCGGTC TTTAGCTGAA 
GAGTATGGTT TGCATACAGT CAACAAAGAT GAAATTATTT CTAGCATGGA CAATCCGGAT 
AATGAGATAG TCCTGTACCT AATGTTGCGG GCTGTTGATC GATTTCATAA GCAGCATGGC 
AGATACCCAG GAGTATCTAA CTATCAAGTT GAAGAAGATA TAGGGAAGTT GAAGTCCTGT 
CTTACTGGCT TCCTTCAGGA ATATGGGTTG TCTGTAATGG TGAAGGACGA TTATGTCCAT 
GAATTTTGCC GGTACGGAGC TGCTGAGCCA CACACCGTTG CTGCATTCCT GGGAGGAGCT 
GCTGCTCAAG AAGTTATCAA AATAATCACC AAACAATTTG TAATTTTTAA CAATACTTAT 
ATTTATAGTG GTATGTCACA AACTTCTGCA ACTTTCCAAT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_114461.3
CDS	44..1648
Translation	MAQQGKILKEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDVGNNFFLQKCSIGKNRAQAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTIVVATQLLESTLLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYSETNGRIPKSYKEKEDFRELIRQGILKNENGAPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSSKYIKLQNVYREKAKKDAAAVGNHVAKLLQSVGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYGLHTVNKDEIISSMDNPDNEIVLYLMLRAVDRFHKQHGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDDYVHEFCRYGAAEPHTVAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL

Target ORF information:

RefSeq Version	NM_032072.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NEDD8 activating enzyme E1 subunit 1 (Nae1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032072.4

ORF Insert Sequence:

ATGGCTCAGC AAGGGAAGAT ACTCAAGGAG CAAAAGTACG ACCGGCAGCT GAGGTTGTGG 
GGTGATCATG GTCAAGAAGC TTTGGAATCT GCTCACGTTT GCTTGATAAA TGCCACAGCT 
ACAGGAACGG AAATTCTTAA AAACTTGGTA CTGCCAGGTA TAGGATCATT TACAATTATC 
GATGGAAATC AGGTCAGCGG AGAAGATGTT GGAAACAATT TTTTCCTTCA AAAATGCAGT 
ATTGGCAAGA ATCGAGCTCA AGCTGCCATG GAATTCTTAC AAGAGCTAAA TAGCGATGTC 
TCTGGAAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGATCC TTCATTTTTC 
TGTAGGTTTA CTATTGTGGT TGCAACTCAG CTTCTTGAAA GTACATTACT ACGTTTAGCA 
GATGTCCTCT GGAATTCCCA GATCCCTCTT TTGATCTGTA GGACATATGG CCTAGTTGGT 
TATATGAGGA TTATTATAAA AGAACATCCA GTAATAGAAT CTCATCCAGA TAATGCATTG 
GAGGACCTAC GACTAGATAA GCCATTTCCT GAACTGAGAG AACATTTTCA GTCTTATGAC 
TTGGACCATA TGGAAAAAAA GGACCATAGC CATACTCCAT GGATTGTGAT CATAGCTAAA 
TATTTAGCCC AGTGGTATAG TGAGACAAAT GGACGAATTC CTAAAAGTTA TAAAGAAAAA 
GAGGACTTCA GAGAATTGAT AAGACAAGGG ATTTTAAAGA ATGAGAATGG AGCTCCAGAA 
GATGAAGAGA ATTTTGAAGA AGCTATTAAA AATGTGAACA CAGCACTAAA TACAACTCAG 
ATCCCAAGCA GTATTGAAGA TATATTTAAT GATGATCGCT GCATAAATAT CACCAAACAG 
ACCCCATCTT TTTGGATTTT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA GGAGGGTCAA 
GGAAATTTAC CTGTTCGAGG CACAATTCCT GATATGATTG CAGATTCAAG CAAATACATA 
AAATTGCAGA ATGTTTATCG TGAGAAAGCA AAGAAAGATG CTGCTGCTGT AGGTAATCAT 
GTTGCCAAAT TGCTTCAGTC TGTTGGCCAG GCACCAGAGT CCATTTCAGA GAAAGAATTA 
AAACTGCTTT GTAGCAATTC TGCATTTCTT CGAGTGGTAA GATGTCGGTC TTTAGCTGAA 
GAGTATGGTT TGCATACAGT CAACAAAGAT GAAATTATTT CTAGCATGGA CAATCCGGAT 
AATGAGATAG TCCTGTACCT AATGTTGCGG GCTGTTGATC GATTTCATAA GCAGCATGGC 
AGATACCCAG GAGTATCTAA CTATCAAGTT GAAGAAGATA TAGGGAAGTT GAAGTCCTGT 
CTTACTGGCT TCCTTCAGGA ATATGGGTTG TCTGTAATGG TGAAGGACGA TTATGTCCAT 
GAATTTTGCC GGTACGGAGC TGCTGAGCCA CACACCGTTG CTGCATTCCT GGGAGGAGCT 
GCTGCTCAAG AAGTTATCAA AATAATCACC AAACAATTTG TAATTTTTAA CAATACTTAT 
ATTTATAGTG GTATGTCACA AACTTCTGCA ACTTTCCAAT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Focal transient ischemia increases APP-BP1 expression in neural progenitor cells.
Neuroreport22(5)200-5(2011 Mar)
Joo Y,Lee SH,Ha S,Hong BH,Kim JH,Suh YH,Kim HS

Amyloid precursor protein binding protein-1 modulates cell cycle progression in fetal neural stem cells.
PloS one5(12)e14203(2010 Dec)
Joo Y,Ha S,Hong BH,Kim Ja,Chang KA,Liew H,Kim S,Sun W,Kim JH,Chong YH,Suh YH,Kim HS

TRIP12 functions as an E3 ubiquitin ligase of APP-BP1.
Biochemical and biophysical research communications374(2)294-8(2008 Sep)
Park Y,Yoon SK,Yoon JB

Rab5 mediates an amyloid precursor protein signaling pathway that leads to apoptosis.
The Journal of neuroscience : the official journal of the Society for Neuroscience27(27)7141-53(2007 Jul)
Laifenfeld D,Patzek LJ,McPhie DL,Chen Y,Levites Y,Cataldo AM,Neve RL

APP-BP1 mediates APP-induced apoptosis and DNA synthesis and is increased in Alzheimer's disease brain.
The Journal of cell biology163(1)27-33(2003 Oct)
Chen Y,Liu W,McPhie DL,Hassinger L,Neve RL