Pias3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pias3
Entrez Gene ID	83614
Full Name	protein inhibitor of activated STAT, 3
General protein information	Preferred Names protein inhibitor of activated STAT, 3 Names E3 SUMO-protein ligase PIAS3 KChAP potassium channel regulatory protein KChAP potassium channel-associated protein protein inhibitor of activated STAT protein 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34
Summary	voltage-gated K+ channel binding protein; involved in modulating the expression of Kv2 channels [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006232991.3	XP_006233053.1	E3 SUMO-protein ligase PIAS3 isoform X1
XM_006232993.3	XP_006233055.1	E3 SUMO-protein ligase PIAS3 isoform X2
XM_008761338.2	XP_008759560.1	E3 SUMO-protein ligase PIAS3 isoform X3
XM_006232992.3	XP_006233054.1	E3 SUMO-protein ligase PIAS3 isoform X2
NM_031784.2	NP_113972.2	E3 SUMO-protein ligase PIAS3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PIAS3	NP_001095655.1
Mus musculus (house mouse)	Pias3	NP_001159421.1
Pan troglodytes (chimpanzee)	PIAS3	XP_003949540.1
	PIAS3	XP_001095153.2
Canis lupus familiaris (dog)	PIAS3	XP_859310.2
Rattus norvegicus (Norway rat)	Pias3	NP_113972.2
Homo sapiens (human)	PIAS3	NP_006090.2
Xenopus tropicalis (tropical clawed frog)	pias3	NP_001116890.1

Gene Ontology
Bibliography

Related articles in PubMed

Characterization of nuclear localization and SUMOylation of the ATBF1 transcription factor in epithelial cells.
Sun X, Li J, Dong FN, Dong JT
PloS one9(3)e92746(2014)

SUMOylation and SUMO-interacting motif (SIM) of metastasis tumor antigen 1 (MTA1) synergistically regulate its transcriptional repressor function.
Cong L, Pakala SB, Ohshiro K, Li DQ, Kumar R
The Journal of biological chemistry286(51)43793-808(2011 Dec)

The PINIT domain of PIAS3: structure-function analysis of its interaction with STAT3.
Mautsa N, Prinsloo E, Bishop ?T, Blatch GL
Journal of molecular recognition : JMR24(5)795-803(2011 Sep-Oct)

A specific epitope of protein inhibitor of activated STAT3 is responsible for the induction of apoptosis in rat transformed mast cells.
Yagil Z, Kay G, Nechushtan H, Razin E
Journal of immunology (Baltimore, Md. : 1950)182(4)2168-75(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We have characterized the biological role of a 23-aa domain derived from PIAS3 that induces apoptosis in these cells by inhibiting the transcriptional activity of both MITF and STAT3.
Title: A specific epitope of protein inhibitor of activated STAT3 is responsible for the induction of apoptosis in rat transformed mast cells.

The following Pias3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pias3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa08730	NM_031784.2 Latest version!	Rattus norvegicus protein inhibitor of activated STAT, 3 (Pias3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa25086	XM_006232991.3 Latest version!	Rattus norvegicus protein inhibitor of activated STAT, 3 (Pias3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa25087	XM_006232992.3 Latest version!	Rattus norvegicus protein inhibitor of activated STAT, 3 (Pias3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa25087	XM_006232993.3 Latest version!	Rattus norvegicus protein inhibitor of activated STAT, 3 (Pias3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa43766	XM_008761338.2 Latest version!	Rattus norvegicus protein inhibitor of activated STAT, 3 (Pias3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pias3 ( NM_031784.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113972.2 Rattus norvegicus protein inhibitor of activated STAT, 3 (Pias3), mRNA.

Price & Availability↑

CloneID	ORa08730
Clone ID Related Accession (Same CDS sequence)	NM_031784.2
Accession Version	NM_031784.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Rattus norvegicus(Norway rat)
Product	E3 SUMO-protein ligase PIAS3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF032872.2. On Dec 4, 2001 this sequence version replaced NM_031784.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF032872.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGAGC TGGGCGAATT AAAGCACATG GTGATGAGTT TCCGGGTGTC TGAGCTCCAG 
GTGCTCCTTG GCTTCGCTGG CAGGAACAAG AGTGGGCGGA AGCACGAATT GCTGGCCAAG 
GCCCTGCACC TCCTCAAGTC TAGCTGCGCC CCCAGCGTCC AGATGAAGAT CAAAGAACTT 
TACCGCAGGC GCTTTCCCCG GAAGACCCTG GGGCCTTCCG ATCTCTCTTT GCTCTCTTTG 
CCCCCTGGCA CCTCTCCTGT AGGCTCCCCC AGCCCCCTTG CTTCCATTCC TCCCACCCTC 
CTGACCCCTG GCACCTTGCT GGGCCCTAAG CGTGAGGTGG ACATGCACCC TCCTCTGCCC 
CAGCCTGTGC ACCCTGACGT CACCATGAAA CCACTGCCCT TCTACGAAGT CTACGGAGAG 
CTCATCCGGC CGACCACCCT TGCGTCCACC TCCAGTCAGA GGTTTGAGGA AGCCCACTTT 
ACCTTTGCAC TCACTCCCCA GCAGCTGCAG CAGATTCTCA CATCCAGGGA GGTTCTGCCA 
GGAGCCAAGT GCGATTATAC CATACAAGTG CAGCTCAGGT TCTGTCTCTG TGAGACCAGC 
TGCCCCCAGG AGGACTATTT CCCCCCTAAC CTCTTTGTCA AGGTTAATGG GAAACTCTGC 
CCCCTGCCGG GTTACCTCCC TCCAACCAAG AATGGAGCTG AGCCCAAGAG GCCTAGTCGT 
CCAATCAACA TCACACCCCT GGCTCGTCTC TCAGCCACTG TTCCCAACAC CATAGTGGTT 
AACTGGTCAT CTGAGTTTGG ACGGAATTAC TCCTTGTCTG TGTACCTGGT GAGGCAGTTG 
ACTGCAGGGA CCCTGCTACA AAAGCTCAGA GCCAAGGGTA TCCGGAATCC AGACCATTCC 
CGAGCACTGA TCAAGGAGAA ATTGACTGCT GACCCCGACA GTGAAGTGGC TACTACAAGT 
CTCCGGGTGT CACTCATGTG CCCGCTGGGG AAGATGCGCC TGACTGTCCC ATGCCGCGCT 
CTCACCTGTG CCCACCTGCA GAGTTTCGAT GCTGCCCTTT ATCTACAGAT GAATGAGAAA 
AAGCCAACAT GGACGTGCCC TGTGTGTGAC AAGAAGGCTC CCTATGAGTC ACTGATTATT 
GATGGTTTAT TCATGGAAAT TCTTAATTCC TGTTCGGATT GTGATGAGAT CCAGTTCATG 
GAAGATGGAT CCTGGTGTCC AATGAAACCC AAGAAGGAGG CATCCGAGGT TTGCCCCCCA 
CCAGGGTATG GGCTGGATGG TCTCCAGTAT AGCCCAGTCC AGGAGGGAAA TCAGTCAGAG 
AATAAGAAGA GGGTTGAAGT CATTGACTTG ACAATCGAAA GCTCATCAGA TGAGGAAGAT 
CTGCCCCCCA CCAAGAAGCA CTGCCCTGTT ACCTCGGCTG CCATTCCAGC CCTTCCTGGA 
AGCAAAGGAG CCCTGACCTC TGGTCACCAG CCGTCTTCGG TGCTGCGGAG CCCTGCAATG 
GGTACACTGG GCAGTGATTT CCTGTCTAGT CTCCCACTAC ATGAGTACCC ACCTGCCTTC 
CCGCTGGGGG CTGACATCCA AGGTTTAGAT TTATTTTCTT TCCTTCAGAC TGAGAGTCAG 
CACTACAGCC CTTCAGTTAT CACTTCACTA GATGAGCAGG ACACCCTTGG CCACTTCTTC 
CAATTCCGGG GAACCCCTCC CCACTTCCTG GGCCCACTGG CCCCCACATT GGGGAGCTCT 
CACCGCAGCG CCACTCCAGC ACCCGCTCCT GGCCGTGTCA GCAGCATTGT GGCTCCTGGG 
AGTTCCTTGA GGGAAGGGCA TGGAGGACCC CTGCCTTCCG GTCCCTCTTT GACTGGCTGT 
CGGTCAGACG TCATTTCCTT GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113972.2
CDS	59..1945
Translation	MAELGELKHMVMSFRVSELQVLLGFAGRNKSGRKHELLAKALHLLKSSCAPSVQMKIKELYRRRFPRKTLGPSDLSLLSLPPGTSPVGSPSPLASIPPTLLTPGTLLGPKREVDMHPPLPQPVHPDVTMKPLPFYEVYGELIRPTTLASTSSQRFEEAHFTFALTPQQLQQILTSREVLPGAKCDYTIQVQLRFCLCETSCPQEDYFPPNLFVKVNGKLCPLPGYLPPTKNGAEPKRPSRPINITPLARLSATVPNTIVVNWSSEFGRNYSLSVYLVRQLTAGTLLQKLRAKGIRNPDHSRALIKEKLTADPDSEVATTSLRVSLMCPLGKMRLTVPCRALTCAHLQSFDAALYLQMNEKKPTWTCPVCDKKAPYESLIIDGLFMEILNSCSDCDEIQFMEDGSWCPMKPKKEASEVCPPPGYGLDGLQYSPVQEGNQSENKKRVEVIDLTIESSSDEEDLPPTKKHCPVTSAAIPALPGSKGALTSGHQPSSVLRSPAMGTLGSDFLSSLPLHEYPPAFPLGADIQGLDLFSFLQTESQHYSPSVITSLDEQDTLGHFFQFRGTPPHFLGPLAPTLGSSHRSATPAPAPGRVSSIVAPGSSLREGHGGPLPSGPSLTGCRSDVISLD

Target ORF information:

RefSeq Version	NM_031784.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein inhibitor of activated STAT, 3 (Pias3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031784.2

ORF Insert Sequence:

ATGGCGGAGC TGGGCGAATT AAAGCACATG GTGATGAGTT TCCGGGTGTC TGAGCTCCAG 
GTGCTCCTTG GCTTCGCTGG CAGGAACAAG AGTGGGCGGA AGCACGAATT GCTGGCCAAG 
GCCCTGCACC TCCTCAAGTC TAGCTGCGCC CCCAGCGTCC AGATGAAGAT CAAAGAACTT 
TACCGCAGGC GCTTTCCCCG GAAGACCCTG GGGCCTTCCG ATCTCTCTTT GCTCTCTTTG 
CCCCCTGGCA CCTCTCCTGT AGGCTCCCCC AGCCCCCTTG CTTCCATTCC TCCCACCCTC 
CTGACCCCTG GCACCTTGCT GGGCCCTAAG CGTGAGGTGG ACATGCACCC TCCTCTGCCC 
CAGCCTGTGC ACCCTGACGT CACCATGAAA CCACTGCCCT TCTACGAAGT CTACGGAGAG 
CTCATCCGGC CGACCACCCT TGCGTCCACC TCCAGTCAGA GGTTTGAGGA AGCCCACTTT 
ACCTTTGCAC TCACTCCCCA GCAGCTGCAG CAGATTCTCA CATCCAGGGA GGTTCTGCCA 
GGAGCCAAGT GCGATTATAC CATACAAGTG CAGCTCAGGT TCTGTCTCTG TGAGACCAGC 
TGCCCCCAGG AGGACTATTT CCCCCCTAAC CTCTTTGTCA AGGTTAATGG GAAACTCTGC 
CCCCTGCCGG GTTACCTCCC TCCAACCAAG AATGGAGCTG AGCCCAAGAG GCCTAGTCGT 
CCAATCAACA TCACACCCCT GGCTCGTCTC TCAGCCACTG TTCCCAACAC CATAGTGGTT 
AACTGGTCAT CTGAGTTTGG ACGGAATTAC TCCTTGTCTG TGTACCTGGT GAGGCAGTTG 
ACTGCAGGGA CCCTGCTACA AAAGCTCAGA GCCAAGGGTA TCCGGAATCC AGACCATTCC 
CGAGCACTGA TCAAGGAGAA ATTGACTGCT GACCCCGACA GTGAAGTGGC TACTACAAGT 
CTCCGGGTGT CACTCATGTG CCCGCTGGGG AAGATGCGCC TGACTGTCCC ATGCCGCGCT 
CTCACCTGTG CCCACCTGCA GAGTTTCGAT GCTGCCCTTT ATCTACAGAT GAATGAGAAA 
AAGCCAACAT GGACGTGCCC TGTGTGTGAC AAGAAGGCTC CCTATGAGTC ACTGATTATT 
GATGGTTTAT TCATGGAAAT TCTTAATTCC TGTTCGGATT GTGATGAGAT CCAGTTCATG 
GAAGATGGAT CCTGGTGTCC AATGAAACCC AAGAAGGAGG CATCCGAGGT TTGCCCCCCA 
CCAGGGTATG GGCTGGATGG TCTCCAGTAT AGCCCAGTCC AGGAGGGAAA TCAGTCAGAG 
AATAAGAAGA GGGTTGAAGT CATTGACTTG ACAATCGAAA GCTCATCAGA TGAGGAAGAT 
CTGCCCCCCA CCAAGAAGCA CTGCCCTGTT ACCTCGGCTG CCATTCCAGC CCTTCCTGGA 
AGCAAAGGAG CCCTGACCTC TGGTCACCAG CCGTCTTCGG TGCTGCGGAG CCCTGCAATG 
GGTACACTGG GCAGTGATTT CCTGTCTAGT CTCCCACTAC ATGAGTACCC ACCTGCCTTC 
CCGCTGGGGG CTGACATCCA AGGTTTAGAT TTATTTTCTT TCCTTCAGAC TGAGAGTCAG 
CACTACAGCC CTTCAGTTAT CACTTCACTA GATGAGCAGG ACACCCTTGG CCACTTCTTC 
CAATTCCGGG GAACCCCTCC CCACTTCCTG GGCCCACTGG CCCCCACATT GGGGAGCTCT 
CACCGCAGCG CCACTCCAGC ACCCGCTCCT GGCCGTGTCA GCAGCATTGT GGCTCCTGGG 
AGTTCCTTGA GGGAAGGGCA TGGAGGACCC CTGCCTTCCG GTCCCTCTTT GACTGGCTGT 
CGGTCAGACG TCATTTCCTT GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pias3 ( XM_006232991.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233053.1 Rattus norvegicus protein inhibitor of activated STAT, 3 (Pias3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa25086
Clone ID Related Accession (Same CDS sequence)	XM_006232991.3
Accession Version	XM_006232991.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 SUMO-protein ligase PIAS3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006232991.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTAT TAGGGCTAGG GATGGGGCTC GGTGGTCCTC GTGTGTTGAG CTGGTCAGTC 
AGGTCAGCTA CTCAGCACAT GGTGATGAGT TTCCGGGTGT CTGAGCTCCA GGTGCTCCTT 
GGCTTCGCTG GCAGGAACAA GAGTGGGCGG AAGCACGAAT TGCTGGCCAA GGCCCTGCAC 
CTCCTCAAGT CTAGCTGCGC CCCCAGCGTC CAGATGAAGA TCAAAGAACT TTACCGCAGG 
CGCTTTCCCC GGAAGACCCT GGGGCCTTCC GATCTCTCTT TGCTCTCTTT GCCCCCTGGC 
ACCTCTCCTG TAGGCTCCCC CAGCCCCCTT GCTTCCATTC CTCCCACCCT CCTGACCCCT 
GGCACCTTGC TGGGCCCTAA GCGTGAGGTG GACATGCACC CTCCTCTGCC CCAGCCTGTG 
CACCCTGACG TCACCATGAA ACCACTGCCC TTCTACGAAG TCTACGGAGA GCTCATCCGG 
CCCACCACCC TTGCGTCCAC CTCCAGTCAG AGGTTTGAGG AAGCCCACTT TACCTTTGCA 
CTCACTCCCC AGCAGCTGCA GCAGATTCTC ACATCCAGGG AGGTTCTGCC AGGAGCCAAG 
TGCGATTATA CCATACAAGT GCAGCTCAGG TTCTGTCTCT GTGAGACCAG CTGCCCCCAG 
GAGGACTATT TCCCCCCTAA CCTCTTTGTC AAGGTTAATG GGAAACTCTG CCCCCTGCCG 
GGTTACCTCC CTCCAACCAA GAATGGAGCT GAGCCCAAGA GGCCTAGTCG TCCAATCAAC 
ATCACACCCC TGGCTCGTCT CTCAGCCACT GTTCCCAACA CCATAGTGGT TAACTGGTCA 
TCTGAGTTTG GACGGAATTA CTCCTTGTCT GTGTACCTGG TGAGGCAGTT GACTGCAGGG 
ACCCTGCTAC AAAAGCTCAG AGCCAAGGGT ATCCGGAATC CAGACCATTC CCGAGCACTG 
ATCAAGGAGA AATTGACTGC TGACCCCGAC AGTGAAGTGG CTACTACAAG TCTCCGGGTG 
TCACTCATGT GCCCGCTGGG GAAGATGCGC CTGACTGTCC CATGCCGCGC TCTCACCTGT 
GCCCACCTGC AGAGTTTCGA TGCTGCCCTT TATCTACAGA TGAATGAGAA AAAGCCAACA 
TGGACGTGCC CTGTGTGTGA CAAGAAGGCT CCCTATGAGT CACTGATTAT TGATGGTTTA 
TTCATGGAAA TTCTTAATTC CTGTTCGGAT TGTGATGAGA TCCAGTTCAT GGAAGATGGA 
TCCTGGTGTC CAATGAAACC CAAGAAGGAG GCATCCGAGG TTTGCCCCCC ACCAGGGTAT 
GGGCTGGATG GTCTCCAGTA TAGCCCAGTC CAGGAGGGAA ATCAGTCAGA GAATAAGAAG 
AGGGTTGAAG TCATTGACTT GACAATCGAA AGCTCATCAG ATGAGGAAGA TCTGCCCCCC 
ACCAAGAAGC ACTGCCCTGT TACCTCGGCT GCCATTCCAG CCCTTCCTGG AAGCAAAGGA 
GCCCTGACCT CTGGTCACCA GCCGTCTTCG GTGCTGCGGA GCCCTGCAAT GGGTACACTG 
GGCAGTGATT TCCTGTCTAG TCTCCCACTA CATGAGTACC CACCTGCCTT CCCGCTGGGG 
GCTGACATCC AAGGTTTAGA TTTATTTTCT TTCCTTCAGA CTGAGAGTCA GCACTACAGC 
CCTTCAGTTA TCACTTCACT AGATGAGCAG GACACCCTTG GCCACTTCTT CCAATTCCGG 
GGAACCCCTC CCCACTTCCT GGGCCCACTG GCCCCCACAT TGGGGAGCTC TCACCGCAGC 
GCCACTCCAG CACCCGCTCC TGGCCGTGTC AGCAGCATTG TGGCTCCTGG GAGTTCCTTG 
AGGGAAGGGC ATGGAGGACC CCTGCCTTCC GGTCCCTCTT TGACTGGCTG TCGGTCAGAC 
GTCATTTCCT TGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233053.1
CDS	262..2199
Translation	MSVLGLGMGLGGPRVLSWSVRSATQHMVMSFRVSELQVLLGFAGRNKSGRKHELLAKALHLLKSSCAPSVQMKIKELYRRRFPRKTLGPSDLSLLSLPPGTSPVGSPSPLASIPPTLLTPGTLLGPKREVDMHPPLPQPVHPDVTMKPLPFYEVYGELIRPTTLASTSSQRFEEAHFTFALTPQQLQQILTSREVLPGAKCDYTIQVQLRFCLCETSCPQEDYFPPNLFVKVNGKLCPLPGYLPPTKNGAEPKRPSRPINITPLARLSATVPNTIVVNWSSEFGRNYSLSVYLVRQLTAGTLLQKLRAKGIRNPDHSRALIKEKLTADPDSEVATTSLRVSLMCPLGKMRLTVPCRALTCAHLQSFDAALYLQMNEKKPTWTCPVCDKKAPYESLIIDGLFMEILNSCSDCDEIQFMEDGSWCPMKPKKEASEVCPPPGYGLDGLQYSPVQEGNQSENKKRVEVIDLTIESSSDEEDLPPTKKHCPVTSAAIPALPGSKGALTSGHQPSSVLRSPAMGTLGSDFLSSLPLHEYPPAFPLGADIQGLDLFSFLQTESQHYSPSVITSLDEQDTLGHFFQFRGTPPHFLGPLAPTLGSSHRSATPAPAPGRVSSIVAPGSSLREGHGGPLPSGPSLTGCRSDVISLD

Target ORF information:

RefSeq Version	XM_006232991.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein inhibitor of activated STAT, 3 (Pias3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232991.3

ORF Insert Sequence:

ATGTCTGTAT TAGGGCTAGG GATGGGGCTC GGTGGTCCTC GTGTGTTGAG CTGGTCAGTC 
AGGTCAGCTA CTCAGCACAT GGTGATGAGT TTCCGGGTGT CTGAGCTCCA GGTGCTCCTT 
GGCTTCGCTG GCAGGAACAA GAGTGGGCGG AAGCACGAAT TGCTGGCCAA GGCCCTGCAC 
CTCCTCAAGT CTAGCTGCGC CCCCAGCGTC CAGATGAAGA TCAAAGAACT TTACCGCAGG 
CGCTTTCCCC GGAAGACCCT GGGGCCTTCC GATCTCTCTT TGCTCTCTTT GCCCCCTGGC 
ACCTCTCCTG TAGGCTCCCC CAGCCCCCTT GCTTCCATTC CTCCCACCCT CCTGACCCCT 
GGCACCTTGC TGGGCCCTAA GCGTGAGGTG GACATGCACC CTCCTCTGCC CCAGCCTGTG 
CACCCTGACG TCACCATGAA ACCACTGCCC TTCTACGAAG TCTACGGAGA GCTCATCCGG 
CCCACCACCC TTGCGTCCAC CTCCAGTCAG AGGTTTGAGG AAGCCCACTT TACCTTTGCA 
CTCACTCCCC AGCAGCTGCA GCAGATTCTC ACATCCAGGG AGGTTCTGCC AGGAGCCAAG 
TGCGATTATA CCATACAAGT GCAGCTCAGG TTCTGTCTCT GTGAGACCAG CTGCCCCCAG 
GAGGACTATT TCCCCCCTAA CCTCTTTGTC AAGGTTAATG GGAAACTCTG CCCCCTGCCG 
GGTTACCTCC CTCCAACCAA GAATGGAGCT GAGCCCAAGA GGCCTAGTCG TCCAATCAAC 
ATCACACCCC TGGCTCGTCT CTCAGCCACT GTTCCCAACA CCATAGTGGT TAACTGGTCA 
TCTGAGTTTG GACGGAATTA CTCCTTGTCT GTGTACCTGG TGAGGCAGTT GACTGCAGGG 
ACCCTGCTAC AAAAGCTCAG AGCCAAGGGT ATCCGGAATC CAGACCATTC CCGAGCACTG 
ATCAAGGAGA AATTGACTGC TGACCCCGAC AGTGAAGTGG CTACTACAAG TCTCCGGGTG 
TCACTCATGT GCCCGCTGGG GAAGATGCGC CTGACTGTCC CATGCCGCGC TCTCACCTGT 
GCCCACCTGC AGAGTTTCGA TGCTGCCCTT TATCTACAGA TGAATGAGAA AAAGCCAACA 
TGGACGTGCC CTGTGTGTGA CAAGAAGGCT CCCTATGAGT CACTGATTAT TGATGGTTTA 
TTCATGGAAA TTCTTAATTC CTGTTCGGAT TGTGATGAGA TCCAGTTCAT GGAAGATGGA 
TCCTGGTGTC CAATGAAACC CAAGAAGGAG GCATCCGAGG TTTGCCCCCC ACCAGGGTAT 
GGGCTGGATG GTCTCCAGTA TAGCCCAGTC CAGGAGGGAA ATCAGTCAGA GAATAAGAAG 
AGGGTTGAAG TCATTGACTT GACAATCGAA AGCTCATCAG ATGAGGAAGA TCTGCCCCCC 
ACCAAGAAGC ACTGCCCTGT TACCTCGGCT GCCATTCCAG CCCTTCCTGG AAGCAAAGGA 
GCCCTGACCT CTGGTCACCA GCCGTCTTCG GTGCTGCGGA GCCCTGCAAT GGGTACACTG 
GGCAGTGATT TCCTGTCTAG TCTCCCACTA CATGAGTACC CACCTGCCTT CCCGCTGGGG 
GCTGACATCC AAGGTTTAGA TTTATTTTCT TTCCTTCAGA CTGAGAGTCA GCACTACAGC 
CCTTCAGTTA TCACTTCACT AGATGAGCAG GACACCCTTG GCCACTTCTT CCAATTCCGG 
GGAACCCCTC CCCACTTCCT GGGCCCACTG GCCCCCACAT TGGGGAGCTC TCACCGCAGC 
GCCACTCCAG CACCCGCTCC TGGCCGTGTC AGCAGCATTG TGGCTCCTGG GAGTTCCTTG 
AGGGAAGGGC ATGGAGGACC CCTGCCTTCC GGTCCCTCTT TGACTGGCTG TCGGTCAGAC 
GTCATTTCCT TGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pias3 ( XM_006232993.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233055.1 Rattus norvegicus protein inhibitor of activated STAT, 3 (Pias3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa25087
Clone ID Related Accession (Same CDS sequence)	XM_006232993.3 , XM_006232992.3
Accession Version	XM_006232993.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 SUMO-protein ligase PIAS3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006232993.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGATGA GTTTCCGGGT GTCTGAGCTC CAGGTGCTCC TTGGCTTCGC TGGCAGGAAC 
AAGAGTGGGC GGAAGCACGA ATTGCTGGCC AAGGCCCTGC ACCTCCTCAA GTCTAGCTGC 
GCCCCCAGCG TCCAGATGAA GATCAAAGAA CTTTACCGCA GGCGCTTTCC CCGGAAGACC 
CTGGGGCCTT CCGATCTCTC TTTGCTCTCT TTGCCCCCTG GCACCTCTCC TGTAGGCTCC 
CCCAGCCCCC TTGCTTCCAT TCCTCCCACC CTCCTGACCC CTGGCACCTT GCTGGGCCCT 
AAGCGTGAGG TGGACATGCA CCCTCCTCTG CCCCAGCCTG TGCACCCTGA CGTCACCATG 
AAACCACTGC CCTTCTACGA AGTCTACGGA GAGCTCATCC GGCCCACCAC CCTTGCGTCC 
ACCTCCAGTC AGAGGTTTGA GGAAGCCCAC TTTACCTTTG CACTCACTCC CCAGCAGCTG 
CAGCAGATTC TCACATCCAG GGAGGTTCTG CCAGGAGCCA AGTGCGATTA TACCATACAA 
GTGCAGCTCA GGTTCTGTCT CTGTGAGACC AGCTGCCCCC AGGAGGACTA TTTCCCCCCT 
AACCTCTTTG TCAAGGTTAA TGGGAAACTC TGCCCCCTGC CGGGTTACCT CCCTCCAACC 
AAGAATGGAG CTGAGCCCAA GAGGCCTAGT CGTCCAATCA ACATCACACC CCTGGCTCGT 
CTCTCAGCCA CTGTTCCCAA CACCATAGTG GTTAACTGGT CATCTGAGTT TGGACGGAAT 
TACTCCTTGT CTGTGTACCT GGTGAGGCAG TTGACTGCAG GGACCCTGCT ACAAAAGCTC 
AGAGCCAAGG GTATCCGGAA TCCAGACCAT TCCCGAGCAC TGATCAAGGA GAAATTGACT 
GCTGACCCCG ACAGTGAAGT GGCTACTACA AGTCTCCGGG TGTCACTCAT GTGCCCGCTG 
GGGAAGATGC GCCTGACTGT CCCATGCCGC GCTCTCACCT GTGCCCACCT GCAGAGTTTC 
GATGCTGCCC TTTATCTACA GATGAATGAG AAAAAGCCAA CATGGACGTG CCCTGTGTGT 
GACAAGAAGG CTCCCTATGA GTCACTGATT ATTGATGGTT TATTCATGGA AATTCTTAAT 
TCCTGTTCGG ATTGTGATGA GATCCAGTTC ATGGAAGATG GATCCTGGTG TCCAATGAAA 
CCCAAGAAGG AGGCATCCGA GGTTTGCCCC CCACCAGGGT ATGGGCTGGA TGGTCTCCAG 
TATAGCCCAG TCCAGGAGGG AAATCAGTCA GAGAATAAGA AGAGGGTTGA AGTCATTGAC 
TTGACAATCG AAAGCTCATC AGATGAGGAA GATCTGCCCC CCACCAAGAA GCACTGCCCT 
GTTACCTCGG CTGCCATTCC AGCCCTTCCT GGAAGCAAAG GAGCCCTGAC CTCTGGTCAC 
CAGCCGTCTT CGGTGCTGCG GAGCCCTGCA ATGGGTACAC TGGGCAGTGA TTTCCTGTCT 
AGTCTCCCAC TACATGAGTA CCCACCTGCC TTCCCGCTGG GGGCTGACAT CCAAGGTTTA 
GATTTATTTT CTTTCCTTCA GACTGAGAGT CAGCACTACA GCCCTTCAGT TATCACTTCA 
CTAGATGAGC AGGACACCCT TGGCCACTTC TTCCAATTCC GGGGAACCCC TCCCCACTTC 
CTGGGCCCAC TGGCCCCCAC ATTGGGGAGC TCTCACCGCA GCGCCACTCC AGCACCCGCT 
CCTGGCCGTG TCAGCAGCAT TGTGGCTCCT GGGAGTTCCT TGAGGGAAGG GCATGGAGGA 
CCCCTGCCTT CCGGTCCCTC TTTGACTGGC TGTCGGTCAG ACGTCATTTC CTTGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233055.1
CDS	314..2173
Translation	MVMSFRVSELQVLLGFAGRNKSGRKHELLAKALHLLKSSCAPSVQMKIKELYRRRFPRKTLGPSDLSLLSLPPGTSPVGSPSPLASIPPTLLTPGTLLGPKREVDMHPPLPQPVHPDVTMKPLPFYEVYGELIRPTTLASTSSQRFEEAHFTFALTPQQLQQILTSREVLPGAKCDYTIQVQLRFCLCETSCPQEDYFPPNLFVKVNGKLCPLPGYLPPTKNGAEPKRPSRPINITPLARLSATVPNTIVVNWSSEFGRNYSLSVYLVRQLTAGTLLQKLRAKGIRNPDHSRALIKEKLTADPDSEVATTSLRVSLMCPLGKMRLTVPCRALTCAHLQSFDAALYLQMNEKKPTWTCPVCDKKAPYESLIIDGLFMEILNSCSDCDEIQFMEDGSWCPMKPKKEASEVCPPPGYGLDGLQYSPVQEGNQSENKKRVEVIDLTIESSSDEEDLPPTKKHCPVTSAAIPALPGSKGALTSGHQPSSVLRSPAMGTLGSDFLSSLPLHEYPPAFPLGADIQGLDLFSFLQTESQHYSPSVITSLDEQDTLGHFFQFRGTPPHFLGPLAPTLGSSHRSATPAPAPGRVSSIVAPGSSLREGHGGPLPSGPSLTGCRSDVISLD

Target ORF information:

RefSeq Version	XM_006232993.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein inhibitor of activated STAT, 3 (Pias3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232993.3

ORF Insert Sequence:

ATGGTGATGA GTTTCCGGGT GTCTGAGCTC CAGGTGCTCC TTGGCTTCGC TGGCAGGAAC 
AAGAGTGGGC GGAAGCACGA ATTGCTGGCC AAGGCCCTGC ACCTCCTCAA GTCTAGCTGC 
GCCCCCAGCG TCCAGATGAA GATCAAAGAA CTTTACCGCA GGCGCTTTCC CCGGAAGACC 
CTGGGGCCTT CCGATCTCTC TTTGCTCTCT TTGCCCCCTG GCACCTCTCC TGTAGGCTCC 
CCCAGCCCCC TTGCTTCCAT TCCTCCCACC CTCCTGACCC CTGGCACCTT GCTGGGCCCT 
AAGCGTGAGG TGGACATGCA CCCTCCTCTG CCCCAGCCTG TGCACCCTGA CGTCACCATG 
AAACCACTGC CCTTCTACGA AGTCTACGGA GAGCTCATCC GGCCCACCAC CCTTGCGTCC 
ACCTCCAGTC AGAGGTTTGA GGAAGCCCAC TTTACCTTTG CACTCACTCC CCAGCAGCTG 
CAGCAGATTC TCACATCCAG GGAGGTTCTG CCAGGAGCCA AGTGCGATTA TACCATACAA 
GTGCAGCTCA GGTTCTGTCT CTGTGAGACC AGCTGCCCCC AGGAGGACTA TTTCCCCCCT 
AACCTCTTTG TCAAGGTTAA TGGGAAACTC TGCCCCCTGC CGGGTTACCT CCCTCCAACC 
AAGAATGGAG CTGAGCCCAA GAGGCCTAGT CGTCCAATCA ACATCACACC CCTGGCTCGT 
CTCTCAGCCA CTGTTCCCAA CACCATAGTG GTTAACTGGT CATCTGAGTT TGGACGGAAT 
TACTCCTTGT CTGTGTACCT GGTGAGGCAG TTGACTGCAG GGACCCTGCT ACAAAAGCTC 
AGAGCCAAGG GTATCCGGAA TCCAGACCAT TCCCGAGCAC TGATCAAGGA GAAATTGACT 
GCTGACCCCG ACAGTGAAGT GGCTACTACA AGTCTCCGGG TGTCACTCAT GTGCCCGCTG 
GGGAAGATGC GCCTGACTGT CCCATGCCGC GCTCTCACCT GTGCCCACCT GCAGAGTTTC 
GATGCTGCCC TTTATCTACA GATGAATGAG AAAAAGCCAA CATGGACGTG CCCTGTGTGT 
GACAAGAAGG CTCCCTATGA GTCACTGATT ATTGATGGTT TATTCATGGA AATTCTTAAT 
TCCTGTTCGG ATTGTGATGA GATCCAGTTC ATGGAAGATG GATCCTGGTG TCCAATGAAA 
CCCAAGAAGG AGGCATCCGA GGTTTGCCCC CCACCAGGGT ATGGGCTGGA TGGTCTCCAG 
TATAGCCCAG TCCAGGAGGG AAATCAGTCA GAGAATAAGA AGAGGGTTGA AGTCATTGAC 
TTGACAATCG AAAGCTCATC AGATGAGGAA GATCTGCCCC CCACCAAGAA GCACTGCCCT 
GTTACCTCGG CTGCCATTCC AGCCCTTCCT GGAAGCAAAG GAGCCCTGAC CTCTGGTCAC 
CAGCCGTCTT CGGTGCTGCG GAGCCCTGCA ATGGGTACAC TGGGCAGTGA TTTCCTGTCT 
AGTCTCCCAC TACATGAGTA CCCACCTGCC TTCCCGCTGG GGGCTGACAT CCAAGGTTTA 
GATTTATTTT CTTTCCTTCA GACTGAGAGT CAGCACTACA GCCCTTCAGT TATCACTTCA 
CTAGATGAGC AGGACACCCT TGGCCACTTC TTCCAATTCC GGGGAACCCC TCCCCACTTC 
CTGGGCCCAC TGGCCCCCAC ATTGGGGAGC TCTCACCGCA GCGCCACTCC AGCACCCGCT 
CCTGGCCGTG TCAGCAGCAT TGTGGCTCCT GGGAGTTCCT TGAGGGAAGG GCATGGAGGA 
CCCCTGCCTT CCGGTCCCTC TTTGACTGGC TGTCGGTCAG ACGTCATTTC CTTGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pias3 ( XM_006232992.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233054.1 Rattus norvegicus protein inhibitor of activated STAT, 3 (Pias3), transcript variant X2, mRNA.

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CloneID	ORa25087
Clone ID Related Accession (Same CDS sequence)	XM_006232993.3 , XM_006232992.3
Accession Version	XM_006232992.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 SUMO-protein ligase PIAS3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006232992.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGATGA GTTTCCGGGT GTCTGAGCTC CAGGTGCTCC TTGGCTTCGC TGGCAGGAAC 
AAGAGTGGGC GGAAGCACGA ATTGCTGGCC AAGGCCCTGC ACCTCCTCAA GTCTAGCTGC 
GCCCCCAGCG TCCAGATGAA GATCAAAGAA CTTTACCGCA GGCGCTTTCC CCGGAAGACC 
CTGGGGCCTT CCGATCTCTC TTTGCTCTCT TTGCCCCCTG GCACCTCTCC TGTAGGCTCC 
CCCAGCCCCC TTGCTTCCAT TCCTCCCACC CTCCTGACCC CTGGCACCTT GCTGGGCCCT 
AAGCGTGAGG TGGACATGCA CCCTCCTCTG CCCCAGCCTG TGCACCCTGA CGTCACCATG 
AAACCACTGC CCTTCTACGA AGTCTACGGA GAGCTCATCC GGCCCACCAC CCTTGCGTCC 
ACCTCCAGTC AGAGGTTTGA GGAAGCCCAC TTTACCTTTG CACTCACTCC CCAGCAGCTG 
CAGCAGATTC TCACATCCAG GGAGGTTCTG CCAGGAGCCA AGTGCGATTA TACCATACAA 
GTGCAGCTCA GGTTCTGTCT CTGTGAGACC AGCTGCCCCC AGGAGGACTA TTTCCCCCCT 
AACCTCTTTG TCAAGGTTAA TGGGAAACTC TGCCCCCTGC CGGGTTACCT CCCTCCAACC 
AAGAATGGAG CTGAGCCCAA GAGGCCTAGT CGTCCAATCA ACATCACACC CCTGGCTCGT 
CTCTCAGCCA CTGTTCCCAA CACCATAGTG GTTAACTGGT CATCTGAGTT TGGACGGAAT 
TACTCCTTGT CTGTGTACCT GGTGAGGCAG TTGACTGCAG GGACCCTGCT ACAAAAGCTC 
AGAGCCAAGG GTATCCGGAA TCCAGACCAT TCCCGAGCAC TGATCAAGGA GAAATTGACT 
GCTGACCCCG ACAGTGAAGT GGCTACTACA AGTCTCCGGG TGTCACTCAT GTGCCCGCTG 
GGGAAGATGC GCCTGACTGT CCCATGCCGC GCTCTCACCT GTGCCCACCT GCAGAGTTTC 
GATGCTGCCC TTTATCTACA GATGAATGAG AAAAAGCCAA CATGGACGTG CCCTGTGTGT 
GACAAGAAGG CTCCCTATGA GTCACTGATT ATTGATGGTT TATTCATGGA AATTCTTAAT 
TCCTGTTCGG ATTGTGATGA GATCCAGTTC ATGGAAGATG GATCCTGGTG TCCAATGAAA 
CCCAAGAAGG AGGCATCCGA GGTTTGCCCC CCACCAGGGT ATGGGCTGGA TGGTCTCCAG 
TATAGCCCAG TCCAGGAGGG AAATCAGTCA GAGAATAAGA AGAGGGTTGA AGTCATTGAC 
TTGACAATCG AAAGCTCATC AGATGAGGAA GATCTGCCCC CCACCAAGAA GCACTGCCCT 
GTTACCTCGG CTGCCATTCC AGCCCTTCCT GGAAGCAAAG GAGCCCTGAC CTCTGGTCAC 
CAGCCGTCTT CGGTGCTGCG GAGCCCTGCA ATGGGTACAC TGGGCAGTGA TTTCCTGTCT 
AGTCTCCCAC TACATGAGTA CCCACCTGCC TTCCCGCTGG GGGCTGACAT CCAAGGTTTA 
GATTTATTTT CTTTCCTTCA GACTGAGAGT CAGCACTACA GCCCTTCAGT TATCACTTCA 
CTAGATGAGC AGGACACCCT TGGCCACTTC TTCCAATTCC GGGGAACCCC TCCCCACTTC 
CTGGGCCCAC TGGCCCCCAC ATTGGGGAGC TCTCACCGCA GCGCCACTCC AGCACCCGCT 
CCTGGCCGTG TCAGCAGCAT TGTGGCTCCT GGGAGTTCCT TGAGGGAAGG GCATGGAGGA 
CCCCTGCCTT CCGGTCCCTC TTTGACTGGC TGTCGGTCAG ACGTCATTTC CTTGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233054.1
CDS	1412..3271
Translation	MVMSFRVSELQVLLGFAGRNKSGRKHELLAKALHLLKSSCAPSVQMKIKELYRRRFPRKTLGPSDLSLLSLPPGTSPVGSPSPLASIPPTLLTPGTLLGPKREVDMHPPLPQPVHPDVTMKPLPFYEVYGELIRPTTLASTSSQRFEEAHFTFALTPQQLQQILTSREVLPGAKCDYTIQVQLRFCLCETSCPQEDYFPPNLFVKVNGKLCPLPGYLPPTKNGAEPKRPSRPINITPLARLSATVPNTIVVNWSSEFGRNYSLSVYLVRQLTAGTLLQKLRAKGIRNPDHSRALIKEKLTADPDSEVATTSLRVSLMCPLGKMRLTVPCRALTCAHLQSFDAALYLQMNEKKPTWTCPVCDKKAPYESLIIDGLFMEILNSCSDCDEIQFMEDGSWCPMKPKKEASEVCPPPGYGLDGLQYSPVQEGNQSENKKRVEVIDLTIESSSDEEDLPPTKKHCPVTSAAIPALPGSKGALTSGHQPSSVLRSPAMGTLGSDFLSSLPLHEYPPAFPLGADIQGLDLFSFLQTESQHYSPSVITSLDEQDTLGHFFQFRGTPPHFLGPLAPTLGSSHRSATPAPAPGRVSSIVAPGSSLREGHGGPLPSGPSLTGCRSDVISLD

Target ORF information:

RefSeq Version	XM_006232992.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein inhibitor of activated STAT, 3 (Pias3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232992.3

ORF Insert Sequence:

ATGGTGATGA GTTTCCGGGT GTCTGAGCTC CAGGTGCTCC TTGGCTTCGC TGGCAGGAAC 
AAGAGTGGGC GGAAGCACGA ATTGCTGGCC AAGGCCCTGC ACCTCCTCAA GTCTAGCTGC 
GCCCCCAGCG TCCAGATGAA GATCAAAGAA CTTTACCGCA GGCGCTTTCC CCGGAAGACC 
CTGGGGCCTT CCGATCTCTC TTTGCTCTCT TTGCCCCCTG GCACCTCTCC TGTAGGCTCC 
CCCAGCCCCC TTGCTTCCAT TCCTCCCACC CTCCTGACCC CTGGCACCTT GCTGGGCCCT 
AAGCGTGAGG TGGACATGCA CCCTCCTCTG CCCCAGCCTG TGCACCCTGA CGTCACCATG 
AAACCACTGC CCTTCTACGA AGTCTACGGA GAGCTCATCC GGCCCACCAC CCTTGCGTCC 
ACCTCCAGTC AGAGGTTTGA GGAAGCCCAC TTTACCTTTG CACTCACTCC CCAGCAGCTG 
CAGCAGATTC TCACATCCAG GGAGGTTCTG CCAGGAGCCA AGTGCGATTA TACCATACAA 
GTGCAGCTCA GGTTCTGTCT CTGTGAGACC AGCTGCCCCC AGGAGGACTA TTTCCCCCCT 
AACCTCTTTG TCAAGGTTAA TGGGAAACTC TGCCCCCTGC CGGGTTACCT CCCTCCAACC 
AAGAATGGAG CTGAGCCCAA GAGGCCTAGT CGTCCAATCA ACATCACACC CCTGGCTCGT 
CTCTCAGCCA CTGTTCCCAA CACCATAGTG GTTAACTGGT CATCTGAGTT TGGACGGAAT 
TACTCCTTGT CTGTGTACCT GGTGAGGCAG TTGACTGCAG GGACCCTGCT ACAAAAGCTC 
AGAGCCAAGG GTATCCGGAA TCCAGACCAT TCCCGAGCAC TGATCAAGGA GAAATTGACT 
GCTGACCCCG ACAGTGAAGT GGCTACTACA AGTCTCCGGG TGTCACTCAT GTGCCCGCTG 
GGGAAGATGC GCCTGACTGT CCCATGCCGC GCTCTCACCT GTGCCCACCT GCAGAGTTTC 
GATGCTGCCC TTTATCTACA GATGAATGAG AAAAAGCCAA CATGGACGTG CCCTGTGTGT 
GACAAGAAGG CTCCCTATGA GTCACTGATT ATTGATGGTT TATTCATGGA AATTCTTAAT 
TCCTGTTCGG ATTGTGATGA GATCCAGTTC ATGGAAGATG GATCCTGGTG TCCAATGAAA 
CCCAAGAAGG AGGCATCCGA GGTTTGCCCC CCACCAGGGT ATGGGCTGGA TGGTCTCCAG 
TATAGCCCAG TCCAGGAGGG AAATCAGTCA GAGAATAAGA AGAGGGTTGA AGTCATTGAC 
TTGACAATCG AAAGCTCATC AGATGAGGAA GATCTGCCCC CCACCAAGAA GCACTGCCCT 
GTTACCTCGG CTGCCATTCC AGCCCTTCCT GGAAGCAAAG GAGCCCTGAC CTCTGGTCAC 
CAGCCGTCTT CGGTGCTGCG GAGCCCTGCA ATGGGTACAC TGGGCAGTGA TTTCCTGTCT 
AGTCTCCCAC TACATGAGTA CCCACCTGCC TTCCCGCTGG GGGCTGACAT CCAAGGTTTA 
GATTTATTTT CTTTCCTTCA GACTGAGAGT CAGCACTACA GCCCTTCAGT TATCACTTCA 
CTAGATGAGC AGGACACCCT TGGCCACTTC TTCCAATTCC GGGGAACCCC TCCCCACTTC 
CTGGGCCCAC TGGCCCCCAC ATTGGGGAGC TCTCACCGCA GCGCCACTCC AGCACCCGCT 
CCTGGCCGTG TCAGCAGCAT TGTGGCTCCT GGGAGTTCCT TGAGGGAAGG GCATGGAGGA 
CCCCTGCCTT CCGGTCCCTC TTTGACTGGC TGTCGGTCAG ACGTCATTTC CTTGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pias3 ( XM_008761338.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759560.1 Rattus norvegicus protein inhibitor of activated STAT, 3 (Pias3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa43766
Clone ID Related Accession (Same CDS sequence)	XM_008761338.2
Accession Version	XM_008761338.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 SUMO-protein ligase PIAS3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761338.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTAT TAGGGCTAGG GATGGGGCTC GGTGGTCCTC GTGTGTTGAG CTGGTCAGTC 
AGGTCAGCTA CTCAGCACAT GGTGATGAGT TTCCGGGTGT CTGAGCTCCA GGTGCTCCTT 
GGCTTCGCTG GCAGGAACAA GAGTGGGCGG AAGCACGAAT TGCTGGCCAA GGCCCTGCAC 
CTCCTCAAGT CTAGCTGCGC CCCCAGCGTC CAGATGAAGA TCAAAGAACT TTACCGCAGG 
CGCTTTCCCC GGAAGACCCT GGGGCCTTCC GATCTCTCTT TGCTCTCTTT GCCCCCTGGC 
ACCTCTCCTG TAGGCTCCCC CAGCCCCCTT GCTTCCATTC CTCCCACCCT CCTGACCCCT 
GGCACCTTGC TGGGCCCTAA GCGTGAGGTG GACATGCACC CTCCTCTGCC CCAGCCTGTG 
CACCCTGACG TCACCATGAA ACCACTGCCC TTCTACGAAG TCTACGGAGA GCTCATCCGG 
CCCACCACCC TTGCGTCCAC CTCCAGTCAG AGGTTTGAGG AAGCCCACTT TACCTTTGCA 
CTCACTCCCC AGCAGCTGCA GCAGATTCTC ACATCCAGGG AGGTTCTGCC AGGAGCCAAG 
TGCGATTATA CCATACAAGT GCAGCTCAGG TTCTGTCTCT GTGAGACCAG CTGCCCCCAG 
GAGGACTATT TCCCCCCTAA CCTCTTTGTC AAGGTTAATG GGAAACTCTG CCCCCTGCCG 
GGTTACCTCC CTCCAACCAA GAATGGAGCT GAGCCCAAGA GGCCTAGTCG TCCAATCAAC 
ATCACACCCC TGGCTCGTCT CTCAGCCACT GTTCCCAACA CCATAGTGGT TAACTGGTCA 
TCTGAGTTTG GACGGAATTA CTCCTTGTCT GTGTACCTGG TGAGGCAGTT GACTGCAGGG 
ACCCTGCTAC AAAAGCTCAG AGCCAAGGGT ATCCGGAATC CAGACCATTC CCGAGCACTG 
ATCAAGGAGA AATTGACTGC TGACCCCGAC AGTGAAGTGG CTACTACAAG TCTCCGGGTG 
TCACTCATGT GCCCGCTGGG GAAGATGCGC CTGACTGTCC CATGCCGCGC TCTCACCTGT 
GCCCACCTGC AGAGTTTCGA TGCTGCCCTT TATCTACAGA TGAATGAGAA AAAGCCAACA 
TGGACGTGCC CTGTGTGTGA CAAGAAGGCT CCCTATGAGT CACTGATTAT TGATGGTTTA 
TTCATGGAAA TTCTTAATTC CTGTTCGGAT TGTGATGAGA TCCAGTTCAT GGAAGATGGA 
TCCTGGTGTC CAATGAAACC CAAGAAGGAG GCATCCGAGG TTTGCCCCCC ACCAGGGTAT 
GGGCTGGATG GTCTCCAGTA TAGCCCAGTC CAGGAGGGAA ATCAGTCAGA GAATAAGAAG 
AGGGTTGAAG TCATTGACTT GACAATCGAA AGCTCATCAG ATGAGGAAGA TCTGCCCCCC 
ACCAAGAAGC ACTGCCCTGT TACCTCGGCT GCCATTCCAG CCCTTCCTGG AAGCAAAGGA 
GCCCTGACCT CTGGTCACCA GCCGTCTTCG GTGCTGCGGA GCCCTGCAAT GGGTACACTG 
GGCAGTGATT TCCTGTCTAG TCTCCCACTA CATGAGTACC CACCTGCCTT CCCGCTGGGG 
GCTGACATCC AAGCACTACA GCCCTTCAGT TATCACTTCA CTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759560.1
CDS	261..1928
Translation	MSVLGLGMGLGGPRVLSWSVRSATQHMVMSFRVSELQVLLGFAGRNKSGRKHELLAKALHLLKSSCAPSVQMKIKELYRRRFPRKTLGPSDLSLLSLPPGTSPVGSPSPLASIPPTLLTPGTLLGPKREVDMHPPLPQPVHPDVTMKPLPFYEVYGELIRPTTLASTSSQRFEEAHFTFALTPQQLQQILTSREVLPGAKCDYTIQVQLRFCLCETSCPQEDYFPPNLFVKVNGKLCPLPGYLPPTKNGAEPKRPSRPINITPLARLSATVPNTIVVNWSSEFGRNYSLSVYLVRQLTAGTLLQKLRAKGIRNPDHSRALIKEKLTADPDSEVATTSLRVSLMCPLGKMRLTVPCRALTCAHLQSFDAALYLQMNEKKPTWTCPVCDKKAPYESLIIDGLFMEILNSCSDCDEIQFMEDGSWCPMKPKKEASEVCPPPGYGLDGLQYSPVQEGNQSENKKRVEVIDLTIESSSDEEDLPPTKKHCPVTSAAIPALPGSKGALTSGHQPSSVLRSPAMGTLGSDFLSSLPLHEYPPAFPLGADIQALQPFSYHFTR

Target ORF information:

RefSeq Version	XM_008761338.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein inhibitor of activated STAT, 3 (Pias3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761338.2

ORF Insert Sequence:

ATGTCTGTAT TAGGGCTAGG GATGGGGCTC GGTGGTCCTC GTGTGTTGAG CTGGTCAGTC 
AGGTCAGCTA CTCAGCACAT GGTGATGAGT TTCCGGGTGT CTGAGCTCCA GGTGCTCCTT 
GGCTTCGCTG GCAGGAACAA GAGTGGGCGG AAGCACGAAT TGCTGGCCAA GGCCCTGCAC 
CTCCTCAAGT CTAGCTGCGC CCCCAGCGTC CAGATGAAGA TCAAAGAACT TTACCGCAGG 
CGCTTTCCCC GGAAGACCCT GGGGCCTTCC GATCTCTCTT TGCTCTCTTT GCCCCCTGGC 
ACCTCTCCTG TAGGCTCCCC CAGCCCCCTT GCTTCCATTC CTCCCACCCT CCTGACCCCT 
GGCACCTTGC TGGGCCCTAA GCGTGAGGTG GACATGCACC CTCCTCTGCC CCAGCCTGTG 
CACCCTGACG TCACCATGAA ACCACTGCCC TTCTACGAAG TCTACGGAGA GCTCATCCGG 
CCCACCACCC TTGCGTCCAC CTCCAGTCAG AGGTTTGAGG AAGCCCACTT TACCTTTGCA 
CTCACTCCCC AGCAGCTGCA GCAGATTCTC ACATCCAGGG AGGTTCTGCC AGGAGCCAAG 
TGCGATTATA CCATACAAGT GCAGCTCAGG TTCTGTCTCT GTGAGACCAG CTGCCCCCAG 
GAGGACTATT TCCCCCCTAA CCTCTTTGTC AAGGTTAATG GGAAACTCTG CCCCCTGCCG 
GGTTACCTCC CTCCAACCAA GAATGGAGCT GAGCCCAAGA GGCCTAGTCG TCCAATCAAC 
ATCACACCCC TGGCTCGTCT CTCAGCCACT GTTCCCAACA CCATAGTGGT TAACTGGTCA 
TCTGAGTTTG GACGGAATTA CTCCTTGTCT GTGTACCTGG TGAGGCAGTT GACTGCAGGG 
ACCCTGCTAC AAAAGCTCAG AGCCAAGGGT ATCCGGAATC CAGACCATTC CCGAGCACTG 
ATCAAGGAGA AATTGACTGC TGACCCCGAC AGTGAAGTGG CTACTACAAG TCTCCGGGTG 
TCACTCATGT GCCCGCTGGG GAAGATGCGC CTGACTGTCC CATGCCGCGC TCTCACCTGT 
GCCCACCTGC AGAGTTTCGA TGCTGCCCTT TATCTACAGA TGAATGAGAA AAAGCCAACA 
TGGACGTGCC CTGTGTGTGA CAAGAAGGCT CCCTATGAGT CACTGATTAT TGATGGTTTA 
TTCATGGAAA TTCTTAATTC CTGTTCGGAT TGTGATGAGA TCCAGTTCAT GGAAGATGGA 
TCCTGGTGTC CAATGAAACC CAAGAAGGAG GCATCCGAGG TTTGCCCCCC ACCAGGGTAT 
GGGCTGGATG GTCTCCAGTA TAGCCCAGTC CAGGAGGGAA ATCAGTCAGA GAATAAGAAG 
AGGGTTGAAG TCATTGACTT GACAATCGAA AGCTCATCAG ATGAGGAAGA TCTGCCCCCC 
ACCAAGAAGC ACTGCCCTGT TACCTCGGCT GCCATTCCAG CCCTTCCTGG AAGCAAAGGA 
GCCCTGACCT CTGGTCACCA GCCGTCTTCG GTGCTGCGGA GCCCTGCAAT GGGTACACTG 
GGCAGTGATT TCCTGTCTAG TCTCCCACTA CATGAGTACC CACCTGCCTT CCCGCTGGGG 
GCTGACATCC AAGCACTACA GCCCTTCAGT TATCACTTCA CTAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of nuclear localization and SUMOylation of the ATBF1 transcription factor in epithelial cells.
PloS one9(3)e92746(2014)
Sun X,Li J,Dong FN,Dong JT

SUMOylation and SUMO-interacting motif (SIM) of metastasis tumor antigen 1 (MTA1) synergistically regulate its transcriptional repressor function.
The Journal of biological chemistry286(51)43793-808(2011 Dec)
Cong L,Pakala SB,Ohshiro K,Li DQ,Kumar R

The PINIT domain of PIAS3: structure-function analysis of its interaction with STAT3.
Journal of molecular recognition : JMR24(5)795-803(2011 Sep-Oct)
Mautsa N,Prinsloo E,Bishop ?T,Blatch GL

A specific epitope of protein inhibitor of activated STAT3 is responsible for the induction of apoptosis in rat transformed mast cells.
Journal of immunology (Baltimore, Md. : 1950)182(4)2168-75(2009 Feb)
Yagil Z,Kay G,Nechushtan H,Razin E

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Pias3 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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