Lypla2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lypla2
Entrez Gene ID	83510
Full Name	lysophospholipase II
General protein information	Preferred Names lysophospholipase II Names acyl-protein thioesterase 2 APT-2 LPL-II lysoPLA II lysophospholipase 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36
Summary	mouse homolog displays preferential activity on zwitterionic lysophospholipid substrates; mouse and rat homolog share the Gly-X-Ser-X-Gly esterase/lipase consensus sequence [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031342.1	NP_112632.1	acyl-protein thioesterase 2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LYPLA2	NP_001155854.1
Drosophila melanogaster (fruit fly)	CG18815	NP_652674.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012126	XP_320405.4
Homo sapiens (human)	LYPLA2	NP_009191.1
Pan troglodytes (chimpanzee)	LYPLA2	NP_001238957.1
Magnaporthe oryzae (rice blast fungus)	MGG_02811	XP_003720928.1
Xenopus tropicalis (tropical clawed frog)	lypla2	NP_989287.1
Bos taurus (cattle)	LYPLA2	NP_001073831.1
Rattus norvegicus (Norway rat)	Lypla2	NP_112632.1
Neurospora crassa	NCU02027	XP_964649.1
Arabidopsis thaliana (thale cress)	AT5G20060	NP_974815.1
	LYPLA2	XP_001112696.2
Canis lupus familiaris (dog)	LYPLA2	XP_003638952.1
Mus musculus (house mouse)	Lypla2	NP_036072.1
Schizosaccharomyces pombe (fission yeast)	SPAC8E11.04c	NP_594165.1
Oryza sativa (rice)	Os01g0175000	NP_001042168.2
Danio rerio (zebrafish)	lypla2	NP_957043.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification and dynamics of the human ZDHHC16-ZDHHC6 palmitoylation cascade.
Abrami L, Dallavilla T, Sandoz PA, Demir M, Kunz B, Savoglidis G, Hatzimanikatis V, van der Goot FG
eLife6(2017 Aug)

Identification of PSD-95 Depalmitoylating Enzymes.
Yokoi N, Fukata Y, Sekiya A, Murakami T, Kobayashi K, Fukata M
The Journal of neuroscience : the official journal of the Society for Neuroscience36(24)6431-44(2016 06)

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Guo Z, Neilson LJ, Zhong H, Murray PS, Zanivan S, Zaidel-Bar R
Science signaling7(354)rs7(2014 Dec)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

Sequence, expression in Escherichia coli, and characterization of lysophospholipase II.
Toyoda T, Sugimoto H, Yamashita S
Biochimica et biophysica acta1437(2)182-93(1999 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Lypla2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lypla2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07871	NM_031342.1 Latest version!	Rattus norvegicus lysophospholipase 2 (Lypla2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lypla2 ( NM_031342.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112632.1 Rattus norvegicus lysophospholipase 2 (Lypla2), mRNA.

Price & Availability↑

CloneID	ORa07871
Clone ID Related Accession (Same CDS sequence)	NM_031342.1
Accession Version	NM_031342.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	acyl-protein thioesterase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB021645.1. ##Evidence-Data-START## Transcript exon combination :: AB021645.1, BC070503.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTGTGGTA ACAACATGTC TGTGCCCCTG CTCACCGACG CAGCCACCGT GTCCGGAGCT 
GAGCGGGAAA CGGCCGCGGT TATTTTTTTA CATGGACTTG GAGACACAGG GCACAGCTGG 
GCTGACGCCC TCTCCACCAT CCGGCTTCCT CATGTCAAGT ACATCTGTCC CCATGCGCCC 
AGGATTCCTG TGACACTCAA CATGAAGATG GTAATGCCCT CCTGGTTTGA CCTGATGGGG 
CTGAGTCCAG ATGCCCCAGA GGATGAAGCT GGCATCAAGA AGGCCGCAGA GAACATCAAG 
GCTTTGATTG AACACGAGAT GAAGAACGGG ATTCCTGCCA ACCGAATCGT CCTGGGTGGC 
TTTTCGCAGG GTGGGGCCCT GTCCCTCTAT ACAGCACTTA CCTGCCCCCA CCCTCTGGCC 
GGCATCGTGG CTTTGAGCTG CTGGCTGCCT CTGCACCGGA ACTTCCCCCA AGCAGCCAAT 
GGCAGTGCCA AGGACCTGGC CATCCTTCAA TGCCACGGCG AGCTGGACCC CATGGTACCT 
GTTCGGTTTG GGGCCCTGAC AGCTGAGAAG CTCCGGACAG TTGTCACACC TGCCAGGGTC 
CAGTTTAAGA CATACCCAGG TGTCATGCAC AGCTCCTGTC CTCAGGAGAT GGCAGCTGTA 
AAGGAATTTC TGGAGAAACT TCTGCCTCCG GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112632.1
CDS	88..783
Translation	MCGNNMSVPLLTDAATVSGAERETAAVIFLHGLGDTGHSWADALSTIRLPHVKYICPHAPRIPVTLNMKMVMPSWFDLMGLSPDAPEDEAGIKKAAENIKALIEHEMKNGIPANRIVLGGFSQGGALSLYTALTCPHPLAGIVALSCWLPLHRNFPQAANGSAKDLAILQCHGELDPMVPVRFGALTAEKLRTVVTPARVQFKTYPGVMHSSCPQEMAAVKEFLEKLLPPV

Target ORF information:

RefSeq Version	NM_031342.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus lysophospholipase 2 (Lypla2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031342.1

ORF Insert Sequence:

ATGTGTGGTA ACAACATGTC TGTGCCCCTG CTCACCGACG CAGCCACCGT GTCCGGAGCT 
GAGCGGGAAA CGGCCGCGGT TATTTTTTTA CATGGACTTG GAGACACAGG GCACAGCTGG 
GCTGACGCCC TCTCCACCAT CCGGCTTCCT CATGTCAAGT ACATCTGTCC CCATGCGCCC 
AGGATTCCTG TGACACTCAA CATGAAGATG GTAATGCCCT CCTGGTTTGA CCTGATGGGG 
CTGAGTCCAG ATGCCCCAGA GGATGAAGCT GGCATCAAGA AGGCCGCAGA GAACATCAAG 
GCTTTGATTG AACACGAGAT GAAGAACGGG ATTCCTGCCA ACCGAATCGT CCTGGGTGGC 
TTTTCGCAGG GTGGGGCCCT GTCCCTCTAT ACAGCACTTA CCTGCCCCCA CCCTCTGGCC 
GGCATCGTGG CTTTGAGCTG CTGGCTGCCT CTGCACCGGA ACTTCCCCCA AGCAGCCAAT 
GGCAGTGCCA AGGACCTGGC CATCCTTCAA TGCCACGGCG AGCTGGACCC CATGGTACCT 
GTTCGGTTTG GGGCCCTGAC AGCTGAGAAG CTCCGGACAG TTGTCACACC TGCCAGGGTC 
CAGTTTAAGA CATACCCAGG TGTCATGCAC AGCTCCTGTC CTCAGGAGAT GGCAGCTGTA 
AAGGAATTTC TGGAGAAACT TCTGCCTCCG GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification and dynamics of the human ZDHHC16-ZDHHC6 palmitoylation cascade.
eLife6(2017 Aug)
Abrami L,Dallavilla T,Sandoz PA,Demir M,Kunz B,Savoglidis G,Hatzimanikatis V,van der Goot FG

Identification of PSD-95 Depalmitoylating Enzymes.
The Journal of neuroscience : the official journal of the Society for Neuroscience36(24)6431-44(2016 06)
Yokoi N,Fukata Y,Sekiya A,Murakami T,Kobayashi K,Fukata M

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Science signaling7(354)rs7(2014 Dec)
Guo Z,Neilson LJ,Zhong H,Murray PS,Zanivan S,Zaidel-Bar R

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

Sequence, expression in Escherichia coli, and characterization of lysophospholipase II.
Biochimica et biophysica acta1437(2)182-93(1999 Feb)
Toyoda T,Sugimoto H,Yamashita S