Dlat cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dlat
Entrez Gene ID	81654
Full Name	dihydrolipoamide S-acetyltransferase
General protein information	Preferred Names dihydrolipoamide S-acetyltransferase Names dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial 70 kDa mitochondrial autoantigen of primary biliary cirrhosis E2 PBC PDC-E2 PDCE2 dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex) dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex pyruvate dehydrogenase complex component E2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q23
Summary	putative dihydrolipoamide acetyltransferase [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031025.1	NP_112287.1	dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial

Pathways from BioSystems

Homologies

Homo sapiens (human)	DLAT	NP_001922.2
Rattus norvegicus (Norway rat)	Dlat	NP_112287.1
Drosophila melanogaster (fruit fly)	CG5261	NP_001260196.1
Magnaporthe oryzae (rice blast fungus)	MGG_09878	XP_003720354.1
Arabidopsis thaliana (thale cress)	AT3G13930	NP_566470.1
Xenopus tropicalis (tropical clawed frog)	dlat	NP_001016320.2
	DLAT	XP_001107013.1
Canis lupus familiaris (dog)	DLAT	XP_546524.2
Mus musculus (house mouse)	Dlat	NP_663589.3
Oryza sativa (rice)	Os07g0410100	NP_001059445.1
Bos taurus (cattle)	DLAT	NP_001192659.2
Gallus gallus (chicken)	DLAT	XP_417933.3
Neurospora crassa	NCU07659	XP_962786.1
Pan troglodytes (chimpanzee)	DLAT	XP_003313345.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007975	XP_317493.4
Caenorhabditis elegans (roundworm)	dlat-1	NP_506579.1
Arabidopsis thaliana (thale cress)	AT1G54220	NP_001031186.1
Danio rerio (zebrafish)	dlat	NP_997832.1
Saccharomyces cerevisiae (baker's yeast)	LAT1	NP_014328.3
Kluyveromyces lactis	KLLA0F04741g	XP_455294.1
Eremothecium gossypii	AGOS_AER364W	NP_985220.1
Schizosaccharomyces pombe (fission yeast)	lat1	NP_587755.1
Oryza sativa (rice)	Os06g0105400	NP_001056555.1
Oryza sativa (rice)	Os02g0105200	NP_001045618.1

Gene Ontology
Bibliography

Related articles in PubMed

Sirtuin 4 is a lipoamidase regulating pyruvate dehydrogenase complex activity.
Mathias RA, Greco TM, Oberstein A, Budayeva HG, Chakrabarti R, Rowland EA, Kang Y, Shenk T, Cristea IM
Cell159(7)1615-25(2014 Dec)

Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
Zhao X, Le?n IR, Bak S, Mogensen M, Wrzesinski K, H?jlund K, Jensen ON
Molecular & cellular proteomics : MCP10(1)M110.000299(2011 Jan)

Structures of the human pyruvate dehydrogenase complex cores: a highly conserved catalytic center with flexible N-terminal domains.
Yu X, Hiromasa Y, Tsen H, Stoops JK, Roche TE, Zhou ZH
Structure (London, England : 1993)16(1)104-14(2008 Jan)

Extended polypeptide linkers establish the spatial architecture of a pyruvate dehydrogenase multienzyme complex.
Lengyel JS, Stott KM, Wu X, Brooks BR, Balbo A, Schuck P, Perham RN, Subramaniam S, Milne JL
Structure (London, England : 1993)16(1)93-103(2008 Jan)

Proteolipid protein is required for transport of sirtuin 2 into CNS myelin.
Werner HB, Kuhlmann K, Shen S, Uecker M, Schardt A, Dimova K, Orfaniotou F, Dhaunchak A, Brinkmann BG, M?bius W, Guarente L, Casaccia-Bonnefil P, Jahn O, Nave KA
The Journal of neuroscience : the official journal of the Society for Neuroscience27(29)7717-30(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dlat gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dlat cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12498	NM_031025.1 Latest version!	Rattus norvegicus dihydrolipoamide S-acetyltransferase (Dlat), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$370.30 $529.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dlat ( NM_031025.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112287.1 Rattus norvegicus dihydrolipoamide S-acetyltransferase (Dlat), mRNA.

Price & Availability↑

CloneID	ORa12498
Clone ID Related Accession (Same CDS sequence)	NM_031025.1
Accession Version	NM_031025.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC107440.1. On Nov 1, 2005 this sequence version replaced XM_343389.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC107440.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTGGCGCG TCTGTGCGCG GAGGGTGCAG AGTGCTGTTC CCAGGGCCGG GTTCAGGGCT 
CGGTGGGCAA CTTTGAAGGG CCCGCGGACC GGCCCCGCCG CAGTTCGTTG CGGCAGTGGG 
ATACCCAGCT ATGGGGTCCG CTCGTTGTGC GGCTGGAGCT ACGGCTCTGC CACCGTCCCA 
CGTAACCGCA TACTGCAACA ACTCCTGGGG TCGCCCAGCC GCCGCTCTTA TAGCCTCCCT 
CCGCACCAGA AGGTTCCACT ACCTTCACTT TCCCCTACAA TGCAGGCAGG CACCATAGCC 
CGCTGGGAAA AAAAAGAAGG GGAAAAAATC AGTGAGGGTG ATCTAATTGC AGAGGTTGAA 
ACTGATAAAG CCACTGTTGG ATTTGAGAGC TTGGAGGAGT GCTATATGGC AAAGATCCTT 
GTTCCTGAGG GGACCAGAGA TGTTCCAGTA GGATCTATCA TTTGTATCAC AGTTGAAAAG 
CCTCAGGATA TTGAGGCCTT TAAAAATTAT ACATTGGATT CAGCAACAGC TGCCACACAG 
GCAGCCCCAG CCCCAGCTGC AGCTCCAGCT GCTGCCCCAG CTGCACCTTC TGCAAGTGCT 
CCAGGTAGCT CCTATCCCGT TCACATGCAG ATTGTTCTTC CTGCCCTCTC CCCAACCATG 
ACCATGGGCA CCGTCCAGAG GTGGGAAAAG AAAGTGGGAG AGAAGCTGAG TGAAGGAGAC 
TTGCTGGCAG AGATAGAGAC CGACAAGGCC ACCATAGGCT TTGAAGTACA AGAAGAAGGT 
TATCTGGCAA AAATCCTGGT CCCTGAAGGC ACAAGGGATG TTCCTCTGGG AACCCCGCTA 
TGTATCATAG TAGAAAAACA GGAAGATATA GCAGCATTTG CAGACTACAG GCCAACAGAA 
GTGACCAGCT TAAAGCCACA GGCACCACCA CCTGTCCCAC CCCCAGTGGC AGCTGTTCCT 
CCCATCCCCC AGCCTTTAGC ACCTACCCCC TCAGCCGCTC CTGCTGGACC AAAGGGAAGG 
GTGTTCGTTA GCCCTCTTGC AAAGAAATTG GCAGCAGAGA AAGGGATTGA CCTCACCCAA 
GTTAAAGGGA CGGGACCAGA AGGCAGAATC ATCAAGAAGG ACATTGACTC TTTTGTGCCT 
ACTAAGGCTG CTCCTGCCGC TGCAGCAGCT GCTCCCCCGG GTCCAAGAGT GGCACCAACT 
CCTGCAGGTG TCTTCATAGA CATCCCCATC AGCAACATTC GTCGAGTGAT TGCGCAGAGG 
CTCATGCAGT CGAAGCAGAC TATACCTCAT TATTACCTTT CTGTTGATGT AAATATGGGA 
GAGGTGCTGT TGGTGCGGAA GGAACTTAAT AAGATGCTTG AAGGTAAAGG AAAAATCTCC 
GTCAATGACT TCATCATAAA AGCTTCAGCT TTGGCCTGCC TGAAAGTTCC TGAAGCAAAC 
TCATCTTGGA TGGACACAGT TATACGACAA AATCATGTGG TTGATGTCAG CGTTGCTGTC 
AGTACCCCTG CAGGACTTAT CACCCCTATT GTGTTTAATG CACACATAAA AGGACTGGAA 
ACCATTGCTA GTGATGTTGT TTCTTTAGCC TCCAAAGCAA GAGAGGGTAA ACTTCAGCCT 
CACGAGTTCC AGGGTGGGAC ATTTACAATC TCCAACTTAG GGATGTTCGG AATTAAGAAT 
TTCTCTGCGA TTATTAACCC ACCTCAGGCA TGTATTTTGG CAATTGGTGC TTCCGAGGAT 
AAACTGATCC CTGCAGATAA TGAGAAAGGC TTTGACGTGG CTAGTGTGAT GTCTGTCACA 
CTCAGCTGTG ATCATCGAGT TGTGGATGGA GCAGTTGGAG CCCAGTGGCT TGCTGAATTC 
AAAAAGTACC TTGAAAAGCC TGTAACTATG TTATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112287.1
CDS	11..1909
Translation	MWRVCARRVQSAVPRAGFRARWATLKGPRTGPAAVRCGSGIPSYGVRSLCGWSYGSATVPRNRILQQLLGSPSRRSYSLPPHQKVPLPSLSPTMQAGTIARWEKKEGEKISEGDLIAEVETDKATVGFESLEECYMAKILVPEGTRDVPVGSIICITVEKPQDIEAFKNYTLDSATAATQAAPAPAAAPAAAPAAPSASAPGSSYPVHMQIVLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKILVPEGTRDVPLGTPLCIIVEKQEDIAAFADYRPTEVTSLKPQAPPPVPPPVAAVPPIPQPLAPTPSAAPAGPKGRVFVSPLAKKLAAEKGIDLTQVKGTGPEGRIIKKDIDSFVPTKAAPAAAAAAPPGPRVAPTPAGVFIDIPISNIRRVIAQRLMQSKQTIPHYYLSVDVNMGEVLLVRKELNKMLEGKGKISVNDFIIKASALACLKVPEANSSWMDTVIRQNHVVDVSVAVSTPAGLITPIVFNAHIKGLETIASDVVSLASKAREGKLQPHEFQGGTFTISNLGMFGIKNFSAIINPPQACILAIGASEDKLIPADNEKGFDVASVMSVTLSCDHRVVDGAVGAQWLAEFKKYLEKPVTMLL

Target ORF information:

RefSeq Version	NM_031025.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dihydrolipoamide S-acetyltransferase (Dlat), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031025.1

ORF Insert Sequence:

ATGTGGCGCG TCTGTGCGCG GAGGGTGCAG AGTGCTGTTC CCAGGGCCGG GTTCAGGGCT 
CGGTGGGCAA CTTTGAAGGG CCCGCGGACC GGCCCCGCCG CAGTTCGTTG CGGCAGTGGG 
ATACCCAGCT ATGGGGTCCG CTCGTTGTGC GGCTGGAGCT ACGGCTCTGC CACCGTCCCA 
CGTAACCGCA TACTGCAACA ACTCCTGGGG TCGCCCAGCC GCCGCTCTTA TAGCCTCCCT 
CCGCACCAGA AGGTTCCACT ACCTTCACTT TCCCCTACAA TGCAGGCAGG CACCATAGCC 
CGCTGGGAAA AAAAAGAAGG GGAAAAAATC AGTGAGGGTG ATCTAATTGC AGAGGTTGAA 
ACTGATAAAG CCACTGTTGG ATTTGAGAGC TTGGAGGAGT GCTATATGGC AAAGATCCTT 
GTTCCTGAGG GGACCAGAGA TGTTCCAGTA GGATCTATCA TTTGTATCAC AGTTGAAAAG 
CCTCAGGATA TTGAGGCCTT TAAAAATTAT ACATTGGATT CAGCAACAGC TGCCACACAG 
GCAGCCCCAG CCCCAGCTGC AGCTCCAGCT GCTGCCCCAG CTGCACCTTC TGCAAGTGCT 
CCAGGTAGCT CCTATCCCGT TCACATGCAG ATTGTTCTTC CTGCCCTCTC CCCAACCATG 
ACCATGGGCA CCGTCCAGAG GTGGGAAAAG AAAGTGGGAG AGAAGCTGAG TGAAGGAGAC 
TTGCTGGCAG AGATAGAGAC CGACAAGGCC ACCATAGGCT TTGAAGTACA AGAAGAAGGT 
TATCTGGCAA AAATCCTGGT CCCTGAAGGC ACAAGGGATG TTCCTCTGGG AACCCCGCTA 
TGTATCATAG TAGAAAAACA GGAAGATATA GCAGCATTTG CAGACTACAG GCCAACAGAA 
GTGACCAGCT TAAAGCCACA GGCACCACCA CCTGTCCCAC CCCCAGTGGC AGCTGTTCCT 
CCCATCCCCC AGCCTTTAGC ACCTACCCCC TCAGCCGCTC CTGCTGGACC AAAGGGAAGG 
GTGTTCGTTA GCCCTCTTGC AAAGAAATTG GCAGCAGAGA AAGGGATTGA CCTCACCCAA 
GTTAAAGGGA CGGGACCAGA AGGCAGAATC ATCAAGAAGG ACATTGACTC TTTTGTGCCT 
ACTAAGGCTG CTCCTGCCGC TGCAGCAGCT GCTCCCCCGG GTCCAAGAGT GGCACCAACT 
CCTGCAGGTG TCTTCATAGA CATCCCCATC AGCAACATTC GTCGAGTGAT TGCGCAGAGG 
CTCATGCAGT CGAAGCAGAC TATACCTCAT TATTACCTTT CTGTTGATGT AAATATGGGA 
GAGGTGCTGT TGGTGCGGAA GGAACTTAAT AAGATGCTTG AAGGTAAAGG AAAAATCTCC 
GTCAATGACT TCATCATAAA AGCTTCAGCT TTGGCCTGCC TGAAAGTTCC TGAAGCAAAC 
TCATCTTGGA TGGACACAGT TATACGACAA AATCATGTGG TTGATGTCAG CGTTGCTGTC 
AGTACCCCTG CAGGACTTAT CACCCCTATT GTGTTTAATG CACACATAAA AGGACTGGAA 
ACCATTGCTA GTGATGTTGT TTCTTTAGCC TCCAAAGCAA GAGAGGGTAA ACTTCAGCCT 
CACGAGTTCC AGGGTGGGAC ATTTACAATC TCCAACTTAG GGATGTTCGG AATTAAGAAT 
TTCTCTGCGA TTATTAACCC ACCTCAGGCA TGTATTTTGG CAATTGGTGC TTCCGAGGAT 
AAACTGATCC CTGCAGATAA TGAGAAAGGC TTTGACGTGG CTAGTGTGAT GTCTGTCACA 
CTCAGCTGTG ATCATCGAGT TGTGGATGGA GCAGTTGGAG CCCAGTGGCT TGCTGAATTC 
AAAAAGTACC TTGAAAAGCC TGTAACTATG TTATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sirtuin 4 is a lipoamidase regulating pyruvate dehydrogenase complex activity.
Cell159(7)1615-25(2014 Dec)
Mathias RA,Greco TM,Oberstein A,Budayeva HG,Chakrabarti R,Rowland EA,Kang Y,Shenk T,Cristea IM

Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
Molecular & cellular proteomics : MCP10(1)M110.000299(2011 Jan)
Zhao X,Le?n IR,Bak S,Mogensen M,Wrzesinski K,H?jlund K,Jensen ON

Structures of the human pyruvate dehydrogenase complex cores: a highly conserved catalytic center with flexible N-terminal domains.
Structure (London, England : 1993)16(1)104-14(2008 Jan)
Yu X,Hiromasa Y,Tsen H,Stoops JK,Roche TE,Zhou ZH

Extended polypeptide linkers establish the spatial architecture of a pyruvate dehydrogenase multienzyme complex.
Structure (London, England : 1993)16(1)93-103(2008 Jan)
Lengyel JS,Stott KM,Wu X,Brooks BR,Balbo A,Schuck P,Perham RN,Subramaniam S,Milne JL

Proteolipid protein is required for transport of sirtuin 2 into CNS myelin.
The Journal of neuroscience : the official journal of the Society for Neuroscience27(29)7717-30(2007 Jul)
Werner HB,Kuhlmann K,Shen S,Uecker M,Schardt A,Dimova K,Orfaniotou F,Dhaunchak A,Brinkmann BG,M?bius W,Guarente L,Casaccia-Bonnefil P,Jahn O,Nave KA

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Dlat cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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