Acta2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Acta2
Entrez Gene ID	81633
Full Name	actin, alpha 2, smooth muscle, aorta
General protein information	Preferred Names actin, alpha 2, smooth muscle, aorta Names actin, aortic smooth muscle alpha-actin-2 smooth muscle alpha-actin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q52
Summary	contractile protein; required for extracellular matrix remodeling [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031004.2	NP_112266.1	actin, aortic smooth muscle

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Acta2	NP_112266.1
Gallus gallus (chicken)	ACTA2	NP_001026400.1
Xenopus tropicalis (tropical clawed frog)	acta1	NP_001006709.1
Homo sapiens (human)	ACTA2	NP_001604.1
Canis lupus familiaris (dog)	ACTA2	XP_534781.2
Bos taurus (cattle)	ACTA2	NP_001029674.1
	ACTA2	XP_002805789.1
Danio rerio (zebrafish)	acta1a	NP_001017750.1
Oryza sativa (rice)	Os12g0163700	NP_001066233.1
Xenopus tropicalis (tropical clawed frog)	MGC75582	NP_989355.1
Pan troglodytes (chimpanzee)	ACTA2	XP_001142014.1
Mus musculus (house mouse)	Acta2	NP_031418.1

Gene Ontology
Bibliography

Related articles in PubMed

miR?21 promotes ??SMA and collagen I expression in hepatic stellate cells via the Smad7 signaling pathway.
Fu RQ, Hu DP, Hu YB, Hong L, Sun QF, Ding JG
Molecular medicine reports16(4)4327-4333(2017 Oct)

Prolactin, EGFR, vimentin and ?-actin profiles in elderly rat prostate subjected to steroid hormonal imbalance.
Hetzl AC, Montico F, Kido LA, Cagnon VH
Tissue & cell48(3)189-96(2016 Jun)

Significance of alpha smooth muscle actin expression in traumatic painful neuromas: a pilot study in rats.
Weng W, Zhao B, Lin D, Gao W, Li Z, Yan H
Scientific reports623828(2016 Mar)

A Targeted Multiple Antigenic Peptide Vaccine Augments the Immune Response to Self TGF-?1 and Suppresses Ongoing Hepatic Fibrosis.
Li Y, Wang W, Jia X, Zhai S, Wang X, Wang Y, Dang S
Archivum immunologiae et therapiae experimentalis63(4)305-15(2015 Aug)

Laser-capture microdissection for analysis of cell type-specific gene expression of muscarinic receptor subtypes in the rat bladder with cyclophosphamide-induced cystitis.
Sugino Y, O'Malley KJ, Wang Z, Tyagi P, Birder LA, Ogawa O, Yoshimura N
International urology and nephrology47(4)637-42(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

considering the steroid hormone imbalance in late life period, as well as, the role of this imbalance in prostatic lesion development and progression, the aim of the study herein was to characterize and correlate prolactin, EGFR, alpha-actin and vimentin immunoreactivity in the prostatic epithelium and stroma of elderly rats, following steroid hormone imbalance.
Title: Prolactin, EGFR, vimentin and ?-actin profiles in elderly rat prostate subjected to steroid hormonal imbalance.

Data suggest that the expression of actin alpha 2 smooth muscle (alpha-SMA) plays certain roles in the neuroma-associated pain, either as a direct cause of pain or as an indirect marker of existence of local mechanical stimuli.
Title: Significance of alpha smooth muscle actin expression in traumatic painful neuromas: a pilot study in rats.

TGF-beta1 and alpha-SMA expression is reduced in a rat model of hepatic fibrosis treated with antigenic peptide vaccine
Title: A Targeted Multiple Antigenic Peptide Vaccine Augments the Immune Response to Self TGF-?1 and Suppresses Ongoing Hepatic Fibrosis.

Expressions of M2R and M3R mRNA were increased in urothelial cells and decreased in detrusor muscles following chronic cystitis.
Title: Laser-capture microdissection for analysis of cell type-specific gene expression of muscarinic receptor subtypes in the rat bladder with cyclophosphamide-induced cystitis.

Smooth muscle alpha-actin is present in the cervix of virgin, 11 day, and 20 day pregnant rats.
Title: The persistence of active smooth muscle in the female rat cervix through pregnancy.

The following Acta2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Acta2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07336	NM_031004.2 Latest version!	Rattus norvegicus actin alpha 2, smooth muscle (Acta2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Acta2 ( NM_031004.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112266.1 Rattus norvegicus actin alpha 2, smooth muscle (Acta2), mRNA.

Price & Availability↑

CloneID	ORa07336
Clone ID Related Accession (Same CDS sequence)	NM_031004.2
Accession Version	NM_031004.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	actin, aortic smooth muscle
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DN934985.1, CB608976.1, BI287899.1 and BI282383.1. On May 25, 2007 this sequence version replaced NM_031004.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC158550.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906347, SAMN01906348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGTGAAG AGGAAGACAG CACAGCTCTG GTGTGTGACA ATGGCTCCGG GCTCTGTAAG 
GCGGGCTTTG CTGGTGATGA TGCTCCCAGG GCTGTTTTCC CATCCATCGT GGGACGTCCC 
AGACACCAGG GAGTGATGGT TGGAATGGGC CAAAAGGACA GCTATGTGGG GGACGAAGCG 
CAGAGCAAGA GAGGGATCCT GACCCTGAAG TATCCGATAG AACACGGCAT CATCACCAAC 
TGGGACGACA TGGAAAAGAT CTGGCACCAC TCCTTCTATA ACGAGCTTCG CGTGGCCCCA 
GAGGAGCATC CGACCTTGCT AACGGAGGCG CCGCTGAACC CTAAGGCCAA CCGGGAGAAA 
ATGACCCAGA TTATGTTTGA GACCTTCAAT GTCCCTGCCA TGTATGTGGC TATTCAGGCT 
GTGCTGTCCC TCTATGCTTC TGGACGTACA ACTGGTATTG TGCTGGACTC TGGAGATGGC 
GTGACTCACA ACGTGCCTAT CTATGAGGGC TATGCTCTGC CTCATGCCAT CATGCGTCTG 
GACTTGGCTG GCCGAGATCT CACCGACTAC CTCATGAAGA TCCTGACTGA GCGTGGCTAT 
TCCTTCGTGA CTACTGCTGA GCGTGAGATC GTCCGTGACA TCAAGGAGAA GCTGTGCTAT 
GTCGCTCTGG ACTTTGAAAA TGAGATGGCC ACTGCTGCTT CCTCTTCTTC CCTGGAGAAG 
AGCTACGAAC TGCCTGATGG GCAGGTGATC ACCATCGGGA ATGAACGCTT CCGCTGCCCG 
GAGACCCTCT TCCAGCCATC TTTCATTGGA ATGGAGTCGG CGGGCATCCA CGAAACCACC 
TATAACAGCA TCATGAAGTG TGATATTGAC ATCAGGAAGG ACCTCTATGC TAACAACGTC 
CTCTCGGGGG GCACTACCAT GTACCCAGGC ATTGCTGACA GGATGCAGAA GGAGATCACA 
GCCCTCGCTC CCAGCACCAT GAAGATCAAG ATTATTGCTC CTCCAGAACG CAAATATTCT 
GTCTGGATCG GCGGCTCCAT CCTGGCTTCT CTATCTACCT TCCAGCAGAT GTGGATCAGC 
AAACAGGAGT ATGACGAAGC TGGCCCCTCC ATTGTCCACC GCAAATGCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112266.1
CDS	58..1191
Translation	MCEEEDSTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF

Target ORF information:

RefSeq Version	NM_031004.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus actin alpha 2, smooth muscle (Acta2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031004.2

ORF Insert Sequence:

ATGTGTGAAG AGGAAGACAG CACAGCTCTG GTGTGTGACA ATGGCTCCGG GCTCTGTAAG 
GCGGGCTTTG CTGGTGATGA TGCTCCCAGG GCTGTTTTCC CATCCATCGT GGGACGTCCC 
AGACACCAGG GAGTGATGGT TGGAATGGGC CAAAAGGACA GCTATGTGGG GGACGAAGCG 
CAGAGCAAGA GAGGGATCCT GACCCTGAAG TATCCGATAG AACACGGCAT CATCACCAAC 
TGGGACGACA TGGAAAAGAT CTGGCACCAC TCCTTCTATA ACGAGCTTCG CGTGGCCCCA 
GAGGAGCATC CGACCTTGCT AACGGAGGCG CCGCTGAACC CTAAGGCCAA CCGGGAGAAA 
ATGACCCAGA TTATGTTTGA GACCTTCAAT GTCCCTGCCA TGTATGTGGC TATTCAGGCT 
GTGCTGTCCC TCTATGCTTC TGGACGTACA ACTGGTATTG TGCTGGACTC TGGAGATGGC 
GTGACTCACA ACGTGCCTAT CTATGAGGGC TATGCTCTGC CTCATGCCAT CATGCGTCTG 
GACTTGGCTG GCCGAGATCT CACCGACTAC CTCATGAAGA TCCTGACTGA GCGTGGCTAT 
TCCTTCGTGA CTACTGCTGA GCGTGAGATC GTCCGTGACA TCAAGGAGAA GCTGTGCTAT 
GTCGCTCTGG ACTTTGAAAA TGAGATGGCC ACTGCTGCTT CCTCTTCTTC CCTGGAGAAG 
AGCTACGAAC TGCCTGATGG GCAGGTGATC ACCATCGGGA ATGAACGCTT CCGCTGCCCG 
GAGACCCTCT TCCAGCCATC TTTCATTGGA ATGGAGTCGG CGGGCATCCA CGAAACCACC 
TATAACAGCA TCATGAAGTG TGATATTGAC ATCAGGAAGG ACCTCTATGC TAACAACGTC 
CTCTCGGGGG GCACTACCAT GTACCCAGGC ATTGCTGACA GGATGCAGAA GGAGATCACA 
GCCCTCGCTC CCAGCACCAT GAAGATCAAG ATTATTGCTC CTCCAGAACG CAAATATTCT 
GTCTGGATCG GCGGCTCCAT CCTGGCTTCT CTATCTACCT TCCAGCAGAT GTGGATCAGC 
AAACAGGAGT ATGACGAAGC TGGCCCCTCC ATTGTCCACC GCAAATGCTT CTAA