Slc6a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc6a1
Entrez Gene ID	79212
Full Name	solute carrier family 6 member 1
Synonyms	Gabt1,Gat1
General protein information	Preferred Names solute carrier family 6 member 1 Names sodium- and chloride-dependent GABA transporter 1 GABA transporter protein GAT-1 solute carrier family 6 (neurotransmitter transporter), member 1 solute carrier family 6 (neurotransmitter transporter, GABA), member 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q42
Summary	transporter for the neurotransmitter gamma-aminobutyric acid (GABA) [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006237060.3	XP_006237122.1	sodium- and chloride-dependent GABA transporter 1 isoform X1
XM_006237059.2	XP_006237121.1	sodium- and chloride-dependent GABA transporter 1 isoform X1
NM_024371.2	NP_077347.1	sodium- and chloride-dependent GABA transporter 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SLC6A1	NP_003033.3
Canis lupus familiaris (dog)	SLC6A1	XP_541770.2
	SLC6A1	XP_001088606.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009768	XP_318854.4
Gallus gallus (chicken)	SLC6A1	XP_414303.1
Pan troglodytes (chimpanzee)	SLC6A1	XP_516280.3
Mus musculus (house mouse)	Slc6a1	NP_848818.1
Danio rerio (zebrafish)	slc6a1a	NP_001038752.2
Drosophila melanogaster (fruit fly)	CG1732	NP_651930.2
Caenorhabditis elegans (roundworm)	snf-11	NP_505873.2
Bos taurus (cattle)	SLC6A1	NP_001071304.1
Rattus norvegicus (Norway rat)	Slc6a1	NP_077347.1
Danio rerio (zebrafish)	slc6a1b	NP_001007363.1
Xenopus tropicalis (tropical clawed frog)	LOC100127682	NP_001106493.1

Gene Ontology
Bibliography

Related articles in PubMed

Localization and expression of GABA transporters in the suprachiasmatic nucleus.
Moldavan M, Cravetchi O, Williams M, Irwin RP, Aicher SA, Allen CN
The European journal of neuroscience42(12)3018-32(2015 Dec)

A quantitative analysis of cellular and synaptic localization of GAT-1 and GAT-3 in rat neocortex.
Melone M, Ciappelloni S, Conti F
Brain structure & function220(2)885-97(2015 Mar)

Synaptic GABA release prevents GABA transporter type-1 reversal during excessive network activity.
Savtchenko L, Megalogeni M, Rusakov DA, Walker MC, Pavlov I
Nature communications66597(2015 Mar)

Inhibition of different GABA transporter systems is required to attenuate epileptiform activity in the CA3 region of the immature rat hippocampus.
Sharopov S, Chen R, Sun H, Kolbaev SN, Kirischuk S, Luhmann HJ, Kilb W
Epilepsy research108(2)182-9(2014 Feb)

Plasma membrane transporters GAT-1 and GAT-3 contribute to heterogeneity of GABAergic synapses in neocortex.
Melone M, Ciappelloni S, Conti F
Frontiers in neuroanatomy872(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study demonstrated that GAT1 and GAT3 were expressed in glial cells but not at detectable levels in suprachiasmatic hypothalamic nucleus neurons
Title: Localization and expression of GABA transporters in the suprachiasmatic nucleus.

Study provides a quantitative analysis of the subcellular distribution of GAT-1 and GAT-3 in cerebral cortex, and a detailed description of their ultrastructural localization at GABAergic synapses.
Title: A quantitative analysis of cellular and synaptic localization of GAT-1 and GAT-3 in rat neocortex.

Epileptiform activity is associated with increased synaptic GABA release and is not accompanied by GAT-1 reversal. Sustained efflux of GABA through GAT-1 is unlikely to occur during physiological or pathological network activity.
Title: Synaptic GABA release prevents GABA transporter type-1 reversal during excessive network activity.

Both Gat-1 and Gat-3 act synergisticaly to regulate excitability in the immature hippocampus.
Title: Inhibition of different GABA transporter systems is required to attenuate epileptiform activity in the CA3 region of the immature rat hippocampus.

An inverse relationship links temperature and substrate apparent affinity in the ion-coupled cotransporters rGAT1 and KAAT1.
Title: An inverse relationship links temperature and substrate apparent affinity in the ion-coupled cotransporters rGAT1 and KAAT1.

The following Slc6a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc6a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa06965	NM_024371.2 Latest version!	Rattus norvegicus solute carrier family 6 member 1 (Slc6a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$349.30 $499.00
ORa27586	XM_006237060.3 Latest version!	Rattus norvegicus solute carrier family 6 member 1 (Slc6a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa27586	XM_006237059.2 Latest version!	Rattus norvegicus solute carrier family 6 member 1 (Slc6a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc6a1 ( NM_024371.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_077347.1 Rattus norvegicus solute carrier family 6 member 1 (Slc6a1), mRNA.

Price & Availability↑

CloneID	ORa06965
Clone ID Related Accession (Same CDS sequence)	NM_024371.2
Accession Version	NM_024371.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Rattus norvegicus(Norway rat)
Product	sodium- and chloride-dependent GABA transporter 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M59742.1. On Feb 21, 2013 this sequence version replaced NM_024371.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M59742.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGACTG ACAACAGCAA GGTGGCTGAT GGGCAGATCT CTACCGAGGT CAGCGAGGCT 
CCTGTGGCCA GTGACAAGCC CAAAACCCTT GTAGTCAAGG TGCAGAAGAA GGCTGGAGAC 
CTCCCTGACC GGGACACATG GAAGGGACGC TTCGACTTCC TCATGTCCTG TGTGGGCTAC 
GCCATCGGCC TGGGCAATGT ATGGAGGTTC CCTTATCTCT GCGGAAAGAA CGGTGGTGGG 
GCCTTCCTAA TTCCATATTT CCTGACGCTC ATCTTTGCGG GTGTTCCTCT CTTCCTTTTG 
GAGTGCTCCC TAGGCCAGTA CACCTCCATT GGGGGCCTGG GCGTATGGAA GCTGGCTCCC 
ATGTTCAAGG GTGTGGGCCT CGCGGCAGCT GTGCTGTCCT TCTGGCTGAA CATTTATTAC 
ATTGTCATCA TCTCCTGGGC CATCTACTAC CTGTACAACT CCTTCACCAC GACTCTGCCG 
TGGAAACAGT GTGACAACCC CTGGAACACT GACCGCTGCT TCTCCAACTA TAGCCTGGTC 
AACACCACCA ACATGACCAG TGCCGTGGTG GAATTCTGGG AGCGCAACAT GCACCAAATG 
ACAGATGGGC TAGACAAGCC AGGACAGATC CGCTGGCCGC TAGCCATCAC ACTGGCCATT 
GCCTGGGTGC TTGTGTATTT CTGCATCTGG AAGGGTGTTG GATGGACTGG AAAGGTGGTC 
TACTTCTCAG CCACATACCC CTACATCATG CTCATCATCC TGTTCTTCCG TGGAGTGACA 
CTTCCTGGGG CCAAGGAGGG GATCCTCTTC TACATCACGC CCAACTTCCG AAAGCTGTCT 
GATTCTGAGG TGTGGCTTGA CGCCGCCACC CAGATCTTCT TCTCCTACGG ACTGGGCCTG 
GGGTCCCTGA TTGCTCTGGG AAGCTACAAC TCTTTCCACA ACAATGTGTA CAGGGACTCC 
ATCATTGTTT GCTGCATCAA CTCCTGCACC AGCATGTTTG CCGGCTTCGT CATCTTCTCC 
ATCGTGGGCT TCATGGCTCA TGTCACCAAG AGGTCCATAG CCGATGTGGC GGCCTCAGGC 
CCTGGACTGG CATTCTTGGC ATACCCCGAG GCTGTGACAC AGCTACCCAT CTCTCCCCTC 
TGGGCTATCC TCTTCTTCTC CATGCTGCTG ATGCTGGGCA TTGACAGCCA GTTCTGTACC 
GTGGAGGGCT TCATCACTGC CCTGGTGGAT GAGTACCCCA GGCTTCTCCG CAATCGCCGT 
GAACTCTTCA TTGCTGCCGT GTGCATCGTG TCCTACCTGA TTGGCCTCTC TAACATCACC 
CAGGGTGGCA TTTATGTCTT CAAACTGTTC GATTATTACT CTGCCAGTGG CATGAGCCTG 
CTGTTCCTGG TTTTCTTTGA GTGTGTCTCC ATTTCCTGGT TTTATGGTGT CAACCGGTTC 
TATGACAACA TCCAGGAGAT GGTTGGCTCC AGGCCCTGCA TCTGGTGGAA GCTGTGCTGG 
TCCTTTTTCA CACCCATCAT TGTGGCGGGC GTGTTTCTCT TCAGTGCTGT GCAGATGACA 
CCACTCACCA TGGGAAGCTA CGTTTTCCCC AAGTGGGGCC AGGGCGTGGG CTGGCTCATG 
GCTCTGTCCT CCATGGTGCT CATCCCCGGG TACATGGCTT ACATGTTCCT CACCCTAAAG 
GGCTCTCTGA AGCAGCGTCT CCAGGTCATG ATTCAGCCCA GTGAAGATAT CGTGCGCCCT 
GAGAATGGTC CTGAGCAGCC GCAGGCTGGC AGCTCAGCCA GCAAGGAGGC CTACATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077347.1
CDS	150..1949
Translation	MATDNSKVADGQISTEVSEAPVASDKPKTLVVKVQKKAGDLPDRDTWKGRFDFLMSCVGYAIGLGNVWRFPYLCGKNGGGAFLIPYFLTLIFAGVPLFLLECSLGQYTSIGGLGVWKLAPMFKGVGLAAAVLSFWLNIYYIVIISWAIYYLYNSFTTTLPWKQCDNPWNTDRCFSNYSLVNTTNMTSAVVEFWERNMHQMTDGLDKPGQIRWPLAITLAIAWVLVYFCIWKGVGWTGKVVYFSATYPYIMLIILFFRGVTLPGAKEGILFYITPNFRKLSDSEVWLDAATQIFFSYGLGLGSLIALGSYNSFHNNVYRDSIIVCCINSCTSMFAGFVIFSIVGFMAHVTKRSIADVAASGPGLAFLAYPEAVTQLPISPLWAILFFSMLLMLGIDSQFCTVEGFITALVDEYPRLLRNRRELFIAAVCIVSYLIGLSNITQGGIYVFKLFDYYSASGMSLLFLVFFECVSISWFYGVNRFYDNIQEMVGSRPCIWWKLCWSFFTPIIVAGVFLFSAVQMTPLTMGSYVFPKWGQGVGWLMALSSMVLIPGYMAYMFLTLKGSLKQRLQVMIQPSEDIVRPENGPEQPQAGSSASKEAYI

Target ORF information:

RefSeq Version	NM_024371.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 6 member 1 (Slc6a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024371.2

ORF Insert Sequence:

ATGGCGACTG ACAACAGCAA GGTGGCTGAT GGGCAGATCT CTACCGAGGT CAGCGAGGCT 
CCTGTGGCCA GTGACAAGCC CAAAACCCTT GTAGTCAAGG TGCAGAAGAA GGCTGGAGAC 
CTCCCTGACC GGGACACATG GAAGGGACGC TTCGACTTCC TCATGTCCTG TGTGGGCTAC 
GCCATCGGCC TGGGCAATGT ATGGAGGTTC CCTTATCTCT GCGGAAAGAA CGGTGGTGGG 
GCCTTCCTAA TTCCATATTT CCTGACGCTC ATCTTTGCGG GTGTTCCTCT CTTCCTTTTG 
GAGTGCTCCC TAGGCCAGTA CACCTCCATT GGGGGCCTGG GCGTATGGAA GCTGGCTCCC 
ATGTTCAAGG GTGTGGGCCT CGCGGCAGCT GTGCTGTCCT TCTGGCTGAA CATTTATTAC 
ATTGTCATCA TCTCCTGGGC CATCTACTAC CTGTACAACT CCTTCACCAC GACTCTGCCG 
TGGAAACAGT GTGACAACCC CTGGAACACT GACCGCTGCT TCTCCAACTA TAGCCTGGTC 
AACACCACCA ACATGACCAG TGCCGTGGTG GAATTCTGGG AGCGCAACAT GCACCAAATG 
ACAGATGGGC TAGACAAGCC AGGACAGATC CGCTGGCCGC TAGCCATCAC ACTGGCCATT 
GCCTGGGTGC TTGTGTATTT CTGCATCTGG AAGGGTGTTG GATGGACTGG AAAGGTGGTC 
TACTTCTCAG CCACATACCC CTACATCATG CTCATCATCC TGTTCTTCCG TGGAGTGACA 
CTTCCTGGGG CCAAGGAGGG GATCCTCTTC TACATCACGC CCAACTTCCG AAAGCTGTCT 
GATTCTGAGG TGTGGCTTGA CGCCGCCACC CAGATCTTCT TCTCCTACGG ACTGGGCCTG 
GGGTCCCTGA TTGCTCTGGG AAGCTACAAC TCTTTCCACA ACAATGTGTA CAGGGACTCC 
ATCATTGTTT GCTGCATCAA CTCCTGCACC AGCATGTTTG CCGGCTTCGT CATCTTCTCC 
ATCGTGGGCT TCATGGCTCA TGTCACCAAG AGGTCCATAG CCGATGTGGC GGCCTCAGGC 
CCTGGACTGG CATTCTTGGC ATACCCCGAG GCTGTGACAC AGCTACCCAT CTCTCCCCTC 
TGGGCTATCC TCTTCTTCTC CATGCTGCTG ATGCTGGGCA TTGACAGCCA GTTCTGTACC 
GTGGAGGGCT TCATCACTGC CCTGGTGGAT GAGTACCCCA GGCTTCTCCG CAATCGCCGT 
GAACTCTTCA TTGCTGCCGT GTGCATCGTG TCCTACCTGA TTGGCCTCTC TAACATCACC 
CAGGGTGGCA TTTATGTCTT CAAACTGTTC GATTATTACT CTGCCAGTGG CATGAGCCTG 
CTGTTCCTGG TTTTCTTTGA GTGTGTCTCC ATTTCCTGGT TTTATGGTGT CAACCGGTTC 
TATGACAACA TCCAGGAGAT GGTTGGCTCC AGGCCCTGCA TCTGGTGGAA GCTGTGCTGG 
TCCTTTTTCA CACCCATCAT TGTGGCGGGC GTGTTTCTCT TCAGTGCTGT GCAGATGACA 
CCACTCACCA TGGGAAGCTA CGTTTTCCCC AAGTGGGGCC AGGGCGTGGG CTGGCTCATG 
GCTCTGTCCT CCATGGTGCT CATCCCCGGG TACATGGCTT ACATGTTCCT CACCCTAAAG 
GGCTCTCTGA AGCAGCGTCT CCAGGTCATG ATTCAGCCCA GTGAAGATAT CGTGCGCCCT 
GAGAATGGTC CTGAGCAGCC GCAGGCTGGC AGCTCAGCCA GCAAGGAGGC CTACATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc6a1 ( XM_006237060.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006237122.1 Rattus norvegicus solute carrier family 6 member 1 (Slc6a1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa27586
Clone ID Related Accession (Same CDS sequence)	XM_006237060.3 , XM_006237059.2
Accession Version	XM_006237060.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sodium- and chloride-dependent GABA transporter 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006237060.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACTG ACAACAGCAA GGTGGCTGAT GGGCAGATCT CTACCGAGGT CAGCGAGGCT 
CCTGTGGCCA GTGACAAGCC CAAAACCCTT GTAGTCAAGG TGCAGAAGAA GGCTGGAGAC 
CTCCCTGACC GGGACACATG GAAGGGACGC TTCGACTTCC TCATGTCCTG TGTGGGCTAC 
GCCATCGGCC TGGGCAATGT ATGGAGGTTC CCTTATCTCT GCGGAAAGAA CGGTGGTGGG 
GCCTTCCTAA TTCCATATTT CCTGACGCTC ATCTTTGCGG GTGTTCCTCT CTTCCTTTTG 
GAGTGCTCCC TAGGCCAGTA CACCTCCATT GGGGGCCTGG GCGTATGGAA GCTGGCTCCC 
ATGTTCAAGG GTGTGGGCCT CGCGGCAGCT GTGCTGTCCT TCTGGCTGAA CATTTATTAC 
ATTGTCATCA TCTCCTGGGC CATCTACTAC CTGTACAACT CCTTCACCAC GACTCTGCCG 
TGGAAACAGT GTGACAACCC CTGGAACACT GACCGCTGCT TCTCCAACTA TAGCCTGGTC 
AACACCACCA ACATGACCAG TGCCGTGGTG GAATTCTGGG AGCGCAACAT GCACCAAATG 
ACAGATGGGC TAGACAAGCC AGGACAGATC CGCTGGCCGC TAGCCATCAC ACTGGCCATT 
GCCTGGGTGC TTGTGTATTT CTGCATCTGG AAGGGTGTTG GATGGACTGG AAAGGTGGTC 
TACTTCTCAG CCACATACCC CTACATCATG CTCATCATCC TGTTCTTCCG TGGAGTGACA 
CTTCCTGGGG CCAAGGAGGG GATCCTCTTC TACATCACGC CCAACTTCCG AAAGCTGTCT 
GATTCTGAGG TGTGGCTTGA CGCCGCCACC CAGATCTTCT TCTCCTACGG ACTGGGCCTG 
GGGTCCCTGA TTGCTCTGGG AAGCTACAAC TCTTTCCACA ACAATGTGTA CAGGGACTCC 
ATCATTGTTT GCTGCATCAA CTCCTGCACC AGCATGTTTG CCGGCTTCGT CATCTTCTCC 
ATCGTGGGCT TCATGGCTCA TGTCACCAAG AGGTCCATAG CCGATGTGGC GGCCTCAGGC 
CCTGGACTGG CATTCTTGGC GTACCCCGAG GCTGTGACAC AGCTACCCAT CTCTCCCCTC 
TGGGCTATCC TCTTCTTCTC CATGCTGCTG ATGCTGGGCA TTGACAGCCA GTTCTGTACC 
GTGGAGGGCT TCATCACTGC CCTGGTGGAT GAGTACCCCA GGCTTCTCCG CAATCGCCGT 
GAACTCTTCA TTGCTGCCGT GTGCATCGTG TCCTACCTGA TTGGCCTCTC TAACATCACC 
CAGGGTGGCA TTTATGTCTT CAAACTGTTC GATTATTACT CTGCCAGTGG CATGAGCCTG 
CTGTTCCTGG TTTTCTTTGA GTGTGTCTCC ATTTCCTGGT TTTATGGTGT CAACCGGTTC 
TATGACAACA TCCAGGAGAT GGTTGGCTCC AGGCCCTGCA TCTGGTGGAA GCTGTGCTGG 
TCCTTTTTCA CACCCATCAT TGTGGCGGGC GTGTTTCTCT TCAGTGCTGT GCAGATGACA 
CCACTCACCA TGGGAAGCTA CGTTTTCCCC AAGTGGGGCC AGGGCGTGGG CTGGCTCATG 
GCTCTGTCCT CCATGGTGCT CATCCCCGGG TACATGGCTT ACATGTTCCT CACCCTAAAG 
GGCTCTCTGA AGCAGCGTCT CCAGGTCATG ATTCAGCCCA GTGAAGATAT CGTGCGCCCT 
GAGAATGGTC CTGAGCAGCC GCAGGCTGGC AGCTCAGCCA GCAAGGAGGC CTACATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006237122.1
CDS	1177..2976
Translation	MATDNSKVADGQISTEVSEAPVASDKPKTLVVKVQKKAGDLPDRDTWKGRFDFLMSCVGYAIGLGNVWRFPYLCGKNGGGAFLIPYFLTLIFAGVPLFLLECSLGQYTSIGGLGVWKLAPMFKGVGLAAAVLSFWLNIYYIVIISWAIYYLYNSFTTTLPWKQCDNPWNTDRCFSNYSLVNTTNMTSAVVEFWERNMHQMTDGLDKPGQIRWPLAITLAIAWVLVYFCIWKGVGWTGKVVYFSATYPYIMLIILFFRGVTLPGAKEGILFYITPNFRKLSDSEVWLDAATQIFFSYGLGLGSLIALGSYNSFHNNVYRDSIIVCCINSCTSMFAGFVIFSIVGFMAHVTKRSIADVAASGPGLAFLAYPEAVTQLPISPLWAILFFSMLLMLGIDSQFCTVEGFITALVDEYPRLLRNRRELFIAAVCIVSYLIGLSNITQGGIYVFKLFDYYSASGMSLLFLVFFECVSISWFYGVNRFYDNIQEMVGSRPCIWWKLCWSFFTPIIVAGVFLFSAVQMTPLTMGSYVFPKWGQGVGWLMALSSMVLIPGYMAYMFLTLKGSLKQRLQVMIQPSEDIVRPENGPEQPQAGSSASKEAYI

Target ORF information:

RefSeq Version	XM_006237060.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 6 member 1 (Slc6a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006237060.3

ORF Insert Sequence:

ATGGCGACTG ACAACAGCAA GGTGGCTGAT GGGCAGATCT CTACCGAGGT CAGCGAGGCT 
CCTGTGGCCA GTGACAAGCC CAAAACCCTT GTAGTCAAGG TGCAGAAGAA GGCTGGAGAC 
CTCCCTGACC GGGACACATG GAAGGGACGC TTCGACTTCC TCATGTCCTG TGTGGGCTAC 
GCCATCGGCC TGGGCAATGT ATGGAGGTTC CCTTATCTCT GCGGAAAGAA CGGTGGTGGG 
GCCTTCCTAA TTCCATATTT CCTGACGCTC ATCTTTGCGG GTGTTCCTCT CTTCCTTTTG 
GAGTGCTCCC TAGGCCAGTA CACCTCCATT GGGGGCCTGG GCGTATGGAA GCTGGCTCCC 
ATGTTCAAGG GTGTGGGCCT CGCGGCAGCT GTGCTGTCCT TCTGGCTGAA CATTTATTAC 
ATTGTCATCA TCTCCTGGGC CATCTACTAC CTGTACAACT CCTTCACCAC GACTCTGCCG 
TGGAAACAGT GTGACAACCC CTGGAACACT GACCGCTGCT TCTCCAACTA TAGCCTGGTC 
AACACCACCA ACATGACCAG TGCCGTGGTG GAATTCTGGG AGCGCAACAT GCACCAAATG 
ACAGATGGGC TAGACAAGCC AGGACAGATC CGCTGGCCGC TAGCCATCAC ACTGGCCATT 
GCCTGGGTGC TTGTGTATTT CTGCATCTGG AAGGGTGTTG GATGGACTGG AAAGGTGGTC 
TACTTCTCAG CCACATACCC CTACATCATG CTCATCATCC TGTTCTTCCG TGGAGTGACA 
CTTCCTGGGG CCAAGGAGGG GATCCTCTTC TACATCACGC CCAACTTCCG AAAGCTGTCT 
GATTCTGAGG TGTGGCTTGA CGCCGCCACC CAGATCTTCT TCTCCTACGG ACTGGGCCTG 
GGGTCCCTGA TTGCTCTGGG AAGCTACAAC TCTTTCCACA ACAATGTGTA CAGGGACTCC 
ATCATTGTTT GCTGCATCAA CTCCTGCACC AGCATGTTTG CCGGCTTCGT CATCTTCTCC 
ATCGTGGGCT TCATGGCTCA TGTCACCAAG AGGTCCATAG CCGATGTGGC GGCCTCAGGC 
CCTGGACTGG CATTCTTGGC GTACCCCGAG GCTGTGACAC AGCTACCCAT CTCTCCCCTC 
TGGGCTATCC TCTTCTTCTC CATGCTGCTG ATGCTGGGCA TTGACAGCCA GTTCTGTACC 
GTGGAGGGCT TCATCACTGC CCTGGTGGAT GAGTACCCCA GGCTTCTCCG CAATCGCCGT 
GAACTCTTCA TTGCTGCCGT GTGCATCGTG TCCTACCTGA TTGGCCTCTC TAACATCACC 
CAGGGTGGCA TTTATGTCTT CAAACTGTTC GATTATTACT CTGCCAGTGG CATGAGCCTG 
CTGTTCCTGG TTTTCTTTGA GTGTGTCTCC ATTTCCTGGT TTTATGGTGT CAACCGGTTC 
TATGACAACA TCCAGGAGAT GGTTGGCTCC AGGCCCTGCA TCTGGTGGAA GCTGTGCTGG 
TCCTTTTTCA CACCCATCAT TGTGGCGGGC GTGTTTCTCT TCAGTGCTGT GCAGATGACA 
CCACTCACCA TGGGAAGCTA CGTTTTCCCC AAGTGGGGCC AGGGCGTGGG CTGGCTCATG 
GCTCTGTCCT CCATGGTGCT CATCCCCGGG TACATGGCTT ACATGTTCCT CACCCTAAAG 
GGCTCTCTGA AGCAGCGTCT CCAGGTCATG ATTCAGCCCA GTGAAGATAT CGTGCGCCCT 
GAGAATGGTC CTGAGCAGCC GCAGGCTGGC AGCTCAGCCA GCAAGGAGGC CTACATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc6a1 ( XM_006237059.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006237121.1 Rattus norvegicus solute carrier family 6 member 1 (Slc6a1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa27586
Clone ID Related Accession (Same CDS sequence)	XM_006237060.3 , XM_006237059.2
Accession Version	XM_006237059.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sodium- and chloride-dependent GABA transporter 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006237059.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACTG ACAACAGCAA GGTGGCTGAT GGGCAGATCT CTACCGAGGT CAGCGAGGCT 
CCTGTGGCCA GTGACAAGCC CAAAACCCTT GTAGTCAAGG TGCAGAAGAA GGCTGGAGAC 
CTCCCTGACC GGGACACATG GAAGGGACGC TTCGACTTCC TCATGTCCTG TGTGGGCTAC 
GCCATCGGCC TGGGCAATGT ATGGAGGTTC CCTTATCTCT GCGGAAAGAA CGGTGGTGGG 
GCCTTCCTAA TTCCATATTT CCTGACGCTC ATCTTTGCGG GTGTTCCTCT CTTCCTTTTG 
GAGTGCTCCC TAGGCCAGTA CACCTCCATT GGGGGCCTGG GCGTATGGAA GCTGGCTCCC 
ATGTTCAAGG GTGTGGGCCT CGCGGCAGCT GTGCTGTCCT TCTGGCTGAA CATTTATTAC 
ATTGTCATCA TCTCCTGGGC CATCTACTAC CTGTACAACT CCTTCACCAC GACTCTGCCG 
TGGAAACAGT GTGACAACCC CTGGAACACT GACCGCTGCT TCTCCAACTA TAGCCTGGTC 
AACACCACCA ACATGACCAG TGCCGTGGTG GAATTCTGGG AGCGCAACAT GCACCAAATG 
ACAGATGGGC TAGACAAGCC AGGACAGATC CGCTGGCCGC TAGCCATCAC ACTGGCCATT 
GCCTGGGTGC TTGTGTATTT CTGCATCTGG AAGGGTGTTG GATGGACTGG AAAGGTGGTC 
TACTTCTCAG CCACATACCC CTACATCATG CTCATCATCC TGTTCTTCCG TGGAGTGACA 
CTTCCTGGGG CCAAGGAGGG GATCCTCTTC TACATCACGC CCAACTTCCG AAAGCTGTCT 
GATTCTGAGG TGTGGCTTGA CGCCGCCACC CAGATCTTCT TCTCCTACGG ACTGGGCCTG 
GGGTCCCTGA TTGCTCTGGG AAGCTACAAC TCTTTCCACA ACAATGTGTA CAGGGACTCC 
ATCATTGTTT GCTGCATCAA CTCCTGCACC AGCATGTTTG CCGGCTTCGT CATCTTCTCC 
ATCGTGGGCT TCATGGCTCA TGTCACCAAG AGGTCCATAG CCGATGTGGC GGCCTCAGGC 
CCTGGACTGG CATTCTTGGC GTACCCCGAG GCTGTGACAC AGCTACCCAT CTCTCCCCTC 
TGGGCTATCC TCTTCTTCTC CATGCTGCTG ATGCTGGGCA TTGACAGCCA GTTCTGTACC 
GTGGAGGGCT TCATCACTGC CCTGGTGGAT GAGTACCCCA GGCTTCTCCG CAATCGCCGT 
GAACTCTTCA TTGCTGCCGT GTGCATCGTG TCCTACCTGA TTGGCCTCTC TAACATCACC 
CAGGGTGGCA TTTATGTCTT CAAACTGTTC GATTATTACT CTGCCAGTGG CATGAGCCTG 
CTGTTCCTGG TTTTCTTTGA GTGTGTCTCC ATTTCCTGGT TTTATGGTGT CAACCGGTTC 
TATGACAACA TCCAGGAGAT GGTTGGCTCC AGGCCCTGCA TCTGGTGGAA GCTGTGCTGG 
TCCTTTTTCA CACCCATCAT TGTGGCGGGC GTGTTTCTCT TCAGTGCTGT GCAGATGACA 
CCACTCACCA TGGGAAGCTA CGTTTTCCCC AAGTGGGGCC AGGGCGTGGG CTGGCTCATG 
GCTCTGTCCT CCATGGTGCT CATCCCCGGG TACATGGCTT ACATGTTCCT CACCCTAAAG 
GGCTCTCTGA AGCAGCGTCT CCAGGTCATG ATTCAGCCCA GTGAAGATAT CGTGCGCCCT 
GAGAATGGTC CTGAGCAGCC GCAGGCTGGC AGCTCAGCCA GCAAGGAGGC CTACATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006237121.1
CDS	351..2150
Translation	MATDNSKVADGQISTEVSEAPVASDKPKTLVVKVQKKAGDLPDRDTWKGRFDFLMSCVGYAIGLGNVWRFPYLCGKNGGGAFLIPYFLTLIFAGVPLFLLECSLGQYTSIGGLGVWKLAPMFKGVGLAAAVLSFWLNIYYIVIISWAIYYLYNSFTTTLPWKQCDNPWNTDRCFSNYSLVNTTNMTSAVVEFWERNMHQMTDGLDKPGQIRWPLAITLAIAWVLVYFCIWKGVGWTGKVVYFSATYPYIMLIILFFRGVTLPGAKEGILFYITPNFRKLSDSEVWLDAATQIFFSYGLGLGSLIALGSYNSFHNNVYRDSIIVCCINSCTSMFAGFVIFSIVGFMAHVTKRSIADVAASGPGLAFLAYPEAVTQLPISPLWAILFFSMLLMLGIDSQFCTVEGFITALVDEYPRLLRNRRELFIAAVCIVSYLIGLSNITQGGIYVFKLFDYYSASGMSLLFLVFFECVSISWFYGVNRFYDNIQEMVGSRPCIWWKLCWSFFTPIIVAGVFLFSAVQMTPLTMGSYVFPKWGQGVGWLMALSSMVLIPGYMAYMFLTLKGSLKQRLQVMIQPSEDIVRPENGPEQPQAGSSASKEAYI

Target ORF information:

RefSeq Version	XM_006237059.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 6 member 1 (Slc6a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006237059.2

ORF Insert Sequence:

ATGGCGACTG ACAACAGCAA GGTGGCTGAT GGGCAGATCT CTACCGAGGT CAGCGAGGCT 
CCTGTGGCCA GTGACAAGCC CAAAACCCTT GTAGTCAAGG TGCAGAAGAA GGCTGGAGAC 
CTCCCTGACC GGGACACATG GAAGGGACGC TTCGACTTCC TCATGTCCTG TGTGGGCTAC 
GCCATCGGCC TGGGCAATGT ATGGAGGTTC CCTTATCTCT GCGGAAAGAA CGGTGGTGGG 
GCCTTCCTAA TTCCATATTT CCTGACGCTC ATCTTTGCGG GTGTTCCTCT CTTCCTTTTG 
GAGTGCTCCC TAGGCCAGTA CACCTCCATT GGGGGCCTGG GCGTATGGAA GCTGGCTCCC 
ATGTTCAAGG GTGTGGGCCT CGCGGCAGCT GTGCTGTCCT TCTGGCTGAA CATTTATTAC 
ATTGTCATCA TCTCCTGGGC CATCTACTAC CTGTACAACT CCTTCACCAC GACTCTGCCG 
TGGAAACAGT GTGACAACCC CTGGAACACT GACCGCTGCT TCTCCAACTA TAGCCTGGTC 
AACACCACCA ACATGACCAG TGCCGTGGTG GAATTCTGGG AGCGCAACAT GCACCAAATG 
ACAGATGGGC TAGACAAGCC AGGACAGATC CGCTGGCCGC TAGCCATCAC ACTGGCCATT 
GCCTGGGTGC TTGTGTATTT CTGCATCTGG AAGGGTGTTG GATGGACTGG AAAGGTGGTC 
TACTTCTCAG CCACATACCC CTACATCATG CTCATCATCC TGTTCTTCCG TGGAGTGACA 
CTTCCTGGGG CCAAGGAGGG GATCCTCTTC TACATCACGC CCAACTTCCG AAAGCTGTCT 
GATTCTGAGG TGTGGCTTGA CGCCGCCACC CAGATCTTCT TCTCCTACGG ACTGGGCCTG 
GGGTCCCTGA TTGCTCTGGG AAGCTACAAC TCTTTCCACA ACAATGTGTA CAGGGACTCC 
ATCATTGTTT GCTGCATCAA CTCCTGCACC AGCATGTTTG CCGGCTTCGT CATCTTCTCC 
ATCGTGGGCT TCATGGCTCA TGTCACCAAG AGGTCCATAG CCGATGTGGC GGCCTCAGGC 
CCTGGACTGG CATTCTTGGC GTACCCCGAG GCTGTGACAC AGCTACCCAT CTCTCCCCTC 
TGGGCTATCC TCTTCTTCTC CATGCTGCTG ATGCTGGGCA TTGACAGCCA GTTCTGTACC 
GTGGAGGGCT TCATCACTGC CCTGGTGGAT GAGTACCCCA GGCTTCTCCG CAATCGCCGT 
GAACTCTTCA TTGCTGCCGT GTGCATCGTG TCCTACCTGA TTGGCCTCTC TAACATCACC 
CAGGGTGGCA TTTATGTCTT CAAACTGTTC GATTATTACT CTGCCAGTGG CATGAGCCTG 
CTGTTCCTGG TTTTCTTTGA GTGTGTCTCC ATTTCCTGGT TTTATGGTGT CAACCGGTTC 
TATGACAACA TCCAGGAGAT GGTTGGCTCC AGGCCCTGCA TCTGGTGGAA GCTGTGCTGG 
TCCTTTTTCA CACCCATCAT TGTGGCGGGC GTGTTTCTCT TCAGTGCTGT GCAGATGACA 
CCACTCACCA TGGGAAGCTA CGTTTTCCCC AAGTGGGGCC AGGGCGTGGG CTGGCTCATG 
GCTCTGTCCT CCATGGTGCT CATCCCCGGG TACATGGCTT ACATGTTCCT CACCCTAAAG 
GGCTCTCTGA AGCAGCGTCT CCAGGTCATG ATTCAGCCCA GTGAAGATAT CGTGCGCCCT 
GAGAATGGTC CTGAGCAGCC GCAGGCTGGC AGCTCAGCCA GCAAGGAGGC CTACATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Localization and expression of GABA transporters in the suprachiasmatic nucleus.
The European journal of neuroscience42(12)3018-32(2015 Dec)
Moldavan M,Cravetchi O,Williams M,Irwin RP,Aicher SA,Allen CN

A quantitative analysis of cellular and synaptic localization of GAT-1 and GAT-3 in rat neocortex.
Brain structure & function220(2)885-97(2015 Mar)
Melone M,Ciappelloni S,Conti F

Synaptic GABA release prevents GABA transporter type-1 reversal during excessive network activity.
Nature communications66597(2015 Mar)
Savtchenko L,Megalogeni M,Rusakov DA,Walker MC,Pavlov I

Inhibition of different GABA transporter systems is required to attenuate epileptiform activity in the CA3 region of the immature rat hippocampus.
Epilepsy research108(2)182-9(2014 Feb)
Sharopov S,Chen R,Sun H,Kolbaev SN,Kirischuk S,Luhmann HJ,Kilb W

Plasma membrane transporters GAT-1 and GAT-3 contribute to heterogeneity of GABAergic synapses in neocortex.
Frontiers in neuroanatomy872(2014)
Melone M,Ciappelloni S,Conti F