Fbn2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fbn2
Entrez Gene ID	689008
Full Name	fibrillin 2
General protein information	Preferred Names fibrillin 2 Names fibrillin-2 fibrillin-2-like
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 18 Map Location: 18q12.1
Summary	extracellular matrix protein which serves as a ligand for alphavbeta3 integrin [RGD, Apr 2007].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031826.1	NP_114014.1	fibrillin-2 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Fbn2	NP_034311.2
Rattus norvegicus (Norway rat)	Fbn2	NP_114014.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010021	XP_553917.3
Homo sapiens (human)	FBN2	NP_001990.2
Bos taurus (cattle)	FBN2	NP_001265517.1
Gallus gallus (chicken)	FBN2	XP_424715.3
Xenopus tropicalis (tropical clawed frog)	fbn2	XP_002931771.2
Pan troglodytes (chimpanzee)	FBN2	XP_526999.3
Canis lupus familiaris (dog)	FBN2	XP_538612.2

Gene Ontology
Bibliography

Related articles in PubMed

Disruption of murine Adamtsl4 results in zonular fiber detachment from the lens and in retinal pigment epithelium dedifferentiation.
Collin GB, Hubmacher D, Charette JR, Hicks WL, Stone L, Yu M, Naggert JK, Krebs MP, Peachey NS, Apte SS, Nishina PM
Human molecular genetics24(24)6958-74(2015 Dec)

Human eye development is characterized by coordinated expression of fibrillin isoforms.
Hubmacher D, Reinhardt DP, Plesec T, Schenke-Layland K, Apte SS
Investigative ophthalmology & visual science55(12)7934-44(2014 Nov)

Rare variants in FBN1 and FBN2 are associated with severe adolescent idiopathic scoliosis.
Buchan JG, Alvarado DM, Haller GE, Cruchaga C, Harms MB, Zhang T, Willing MC, Grange DK, Braverman AC, Miller NH, Morcuende JA, Tang NL, Lam TP, Ng BK, Cheng JC, Dobbs MB, Gurnett CA
Human molecular genetics23(19)5271-82(2014 Oct)

Comparative proteomic analysis of supportive and unsupportive extracellular matrix substrates for human embryonic stem cell maintenance.
Soteriou D, Iskender B, Byron A, Humphries JD, Borg-Bartolo S, Haddock MC, Baxter MA, Knight D, Humphries MJ, Kimber SJ
The Journal of biological chemistry288(26)18716-31(2013 Jun)

A trans-acting protein effect causes severe eye malformation in the Mp mouse.
Rainger J, Keighren M, Keene DR, Charbonneau NL, Rainger JK, Fisher M, Mella S, Huang JT, Rose L, van't Hof R, Sakai LY, Jackson IJ, Fitzpatrick DR
PLoS genetics9(12)e1003998(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Fbn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fbn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08825	NM_031826.1 Latest version!	Rattus norvegicus fibrillin 2 (Fbn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fbn2 ( NM_031826.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_114014.1 Rattus norvegicus fibrillin 2 (Fbn2), mRNA.

Price & Availability↑

CloneID	ORa08825
Clone ID Related Accession (Same CDS sequence)	NM_031826.1
Accession Version	NM_031826.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-26
Organism	Rattus norvegicus(Norway rat)
Product	fibrillin-2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF135060.1. On or before Apr 26, 2007 this sequence version replaced XM_001069157.1, XM_577627.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF135060.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGGGAGAA GACGGAGGCT GTGTCTCCGG CCCTACTTCG TGTGGCTGGG TTGCGTGGCG 
CTCTGGGCGC AGGGCACAGA TGGCCAGCCC CAGCCTCCTC CACCAAAGAC GCTCCGGCCC 
CAGCCGCCCC CGCAACAGGT CCGGCCCGCA GGAGCGTCCG AAGGTGGCTT CGCGGGGCCT 
GAGTACAGGG ACGAGGGCGC GTTAGCCGCG AGCCGGGTCC GCAGGCGAGG ACAGCAGGAA 
ATACTCCGAG GGCCCAACGT GTGCGGTTCT AGATTCCACT CCTATTGCTG CCCGGGATGG 
AAGACGCTCC CAGGAGGAAA TCAGTGCATT GTCCCCATTT GCAGGAACAG CTGTGGAGAT 
GGATTCTGTT CCCGCCCCAA CATGTGTACC TGTTCCAGCG GACAAATATC CCCGACCTGT 
GGAGGCAAAT CAATTCAGCA GTGTAGTGTG AGGTGCATGA ACGGTGGGAC CTGTGCGGAT 
GACCACTGTC AGTGCCAGAA GGGATACATC GGGACCTACT GTGGACAACC TGTCTGTGAG 
ACTGGATGCC AGAATGGTGG GCGTTGCATC GGCCCTAACC GCTGTGCTTG TGTTTATGGA 
TTCACCGGGC CACAGTGCGA AAGAGATTAC AGGACAGGCC CATGTTTCAC TCAAGTCAAT 
AATCAGATGT GCCAGGGGCA GCTGACAGGC ATCGTCTGTA CGAAGACGCT GTGCTGTGCC 
ACCATTGGGC GAGCCTGGGG CCATCCTTGT GAGATGTGCC CAGCCCAGCC TCAGCCCTGC 
CGCCGGGGCT TCATCCCCAA CATCCGCACT GGAGCATGTC AGGATGTTGA CGAATGCCAG 
GCGATTCCAG GATTGTGCCA GGGCGGAAAC TGTATCAACA CAGTGGGCTC TTTTGAATGC 
AGATGCCCTG CTGGCCACAA GCAGAGTGAA ACCACACAGA AATGTGAAGA CATCGACGAG 
TGCAGCGTGG TTCCTGGAAT CTGTGAGACA GGCGACTGTT CCAACACCGT GGGAAGCTAT 
TTTTGTCTCT GTCCACGCGG CTTCGTTACC TCAACAGATG GCTCCCGGTG CATTGATCAG 
AGAACTGGCA CATGCTTCTC CGGCCTGGTG AATGGCCGCT GTGCTCAAGA ACTCCCAGGC 
AGAATGGCAA AAGCTCAGTG CTGTTGCGAG CCCGGCCGTT GCTGGGGCAT CGGGACCATT 
CCTGAGGCCT GTCCTGTCAG AGGTTCTGAA GAGTACCGTA GACTGTGCCT GGATGGTCTT 
CCAATGGGCG GTATTCCGGG GAGTTCAGTT TCTAGACCTG GAGGGTCTGG GAGCAACAGC 
AATGGTTATG GCCCAGGAGG AACAGGCTTC CTGCCCATCC CTGGAGGCAA TGGCTTTTCT 
CCTGGTGTTG GAGGAGCTGG AGTAGGAGCT GGAGGGCAGG GACCCATCAT CACGGGACTA 
ACAATTCTCA ACCAGACAAT AGACATCTGT AAGCACCATG CCAATCTGTG TTTGAACGGA 
CGTTGCATAC CTACTGTTTC CAGCTACAGA TGCGAATGTA ACATGGGCTA CAAGCAGGAT 
GCTAATGGAG ATTGTATAGA TGTTGATGAA TGTACATCAA ACCCCTGCAG TCATGGAGAT 
TGTGTTAACA CACCTGGATC CTATTACTGT AAATGTCACG CTGGATTCCA GAGGACTCCT 
ACGAAGCAAG CATGTATCGA CATTGATGAG TGCATCCAGA ATGGGGTTCT GTGTAAAAAT 
GGCCGATGCG TGAACACAGA TGGCAGCTTC CAGTGCATTT GCAATGCTGG CTTTGAGTTA 
ACCACAGATG GAGAAAACTG TGTTGGCCAC GATGAATGTA CAACGACCAA CATGTGCTTG 
AACGGGATGT GTATCAATGA AGATGGCAGC TTCAAGTGCG TGTGCAAGCC AGGCTTTGTC 
CTGGCTCCAA ATGGCCGCTG CTGCACAGAC GTGGACGAGT GCCAGACCCC GGGCATCTGC 
ATGAATGGGC ACTGCATCAA CAACGAAGGG TCCTTCCGCT GTGACTGTCC TCCCGGCCTG 
GCTGTGGGTG TGGATGGACG TGTATGTGTT GACACGCATA TGCGTAGCAC CTGCTATGGG 
GAAATCAAGA AAGGAGTGTG TGTGCGGCCA TTCCCGGGTG CAGTGACCAA GTATGAATGC 
TGCTGTGCTA ATCCAGACTA TGGTTTTGGA GAGCCCTGCC AGCCTTGTCC TGCTAAAAAT 
TCAGCGGAGT TCCATGGTCT TTGTAGCGGT GGCGTAGGCA TCACTGTGGA TGGAAGAGAT 
ATCAATGAGT GTGCTTTGGA TCCTGACATA TGTGCCAACG GGATCTGTGA AAACTTGCGA 
GGCAGCTACC GGTGTAACTG CAACAGTGGC TATGAGCCAG ACGCCTCAGG AAGAAATTGC 
ATTGACATTG ATGAGTGCTT GGTAAACAGG CTGCTTTGTG ACAATGGCCT ATGCAGAAAT 
ACGCCTGGCA GTTACAGCTG CACGTGTCCC CCTGGCTATG TGTTCCGGAC AGAGACAGAG 
ACCTGCGAAG ATGTCAATGA GTGTGAAAGC AACCCGTGTG TCAATGGAGC CTGCAGGAAC 
AACCTGGGCT CTTTCCACTG CGAGTGCTCG CCTGGGAGCA AGCTCAGCTC CACGGGACTG 
ATCTGCATCG GCAGCCTGAA AGGGACCTGC TGGCTCAACA TCCAGGACAA CCGCTGCGAA 
GTGAACATTA ACGGAGCCAC CCTGAAATCT GAATGCTGCG CCACGCTTGG AACTGCCTGG 
GGGAGCCCCT GTGAGCGCTG CGAGCTAGAT GCAGCTTGCC CGAGAGGGTT CGCCAGGATC 
AAAGGTGTGA CCTGCGAAGA CGTTAATGAG TGCGAGGTGT TTCCTGGCGT CTGTCCAAAT 
GGACGCTGTG TCAACAACAA AGGCTCTTTC CACTGTGAAT GCCCTGAAGG CCTTACTCTG 
GATGGCACAG GCCGGGTGTG TCTGGATGTC CGCATGGAGC ACTGCTTCCT GAAGTGGGAT 
GAGGATGAGT GCGTCCACCC CGTCCCCGGG AAGTTCCGCA TGGACGCCTG CTGCTGTGCG 
GTCGGGGCGG CTTGGGGCAC AGAGTGTGAA GAGTGTCCAA AACCTGGCAC CAAGGAATAT 
GAGACCCTGT GTCCCCGGGG CCCCGGCTTT GCTAACAGAG GGGACATCCT CACTGGGCGT 
CCATTTTACA AAGATATCAA CGAGTGCAAG GCCTTGCCTG GGATGTGCAC CTATGGGAAG 
TGTAGAAACA CAATCGGAAG CTTCAAGTGT CGTTGCAACA GTGGCTTTGC CCTGGACATG 
GAGGAGAGGA ACTGTACTGA CATTGATGAG TGCAGGATCT CCCCTGACCT CTGTGGCAAT 
GGCATCTGTG TCAACACGCC AGGCAGCTTT GAGTGCGAGT GCTTTGAGGG CTACGAGAGC 
GGCTTCATGA TGATGAAGAA TTGCATGGAC ATTGACGAAT GTGAACGTAA CCCTCTCCTT 
TGTAGGGGTG GCACCTGTGT GAACACTGAG GGAAGCTTTC AGTGTGACTG CCCACTGGGA 
CACGAGCTGT CGCCGTCACG CGAGGACTGC ATAGATATTA ACGAGTGCTC CCTGAGCGAC 
AATCTCTGCA GAAATGGCAA ATGTGTGAAC ATGATCGGAA CCTACCAGTG TTCTTGCAAT 
CCTGGATACC AGGCCACGCC AGATCGCCAG GGCTGTTCAG ATATCGATGA GTGTATGATA 
ATGAACGGGG GCTGCGACAC CCAGTGCACC AACTCAGAAG GAAGCTACGA ATGCAGCTGC 
AGCGAAGGGT ACGCACTGAT GCCAGACGGA CGGTCGTGCG CAGATATTGA CGAGTGTGAA 
AACAACCCCG ACATCTGTGA CGGCGGCCAG TGTACCAACA TTCCGGGGGA GTATCGCTGC 
CTCTGCTACG ATGGCTTCAT GGCGTCGATG GACATGAAGA CATGCATCGA TGTGAACGAG 
TGTGACCTGA ATCCCAACAT CTGCATGTTT GGGGAGTGTG AGAACACAAA GGGCTCCTTC 
ATTTGCCACT GCCAGCTGGG CTACTCAGTG AAGAAGGGGG CCACGGGATG CACAGACGTG 
GACGAGTGTG AAATTGGCGC CCACAACTGT GACATGCACG CCTCGTGCCT GAATGTCCCT 
GGGAGCTTCA AATGCAGCTG CAGAGAAGGC TGGGTGGGAA ATGGCATCAA ATGCATTGAT 
CTGGATGAAT GCGCGAATGG AACCCACCAG TGCAGCATAA ACGCCCAGTG TGTCAACACC 
CCAGGCTCGT ACCGATGTGC CTGCTCGGAG GGTTTCACGG GAGACGGCTT TACTTGCTCA 
GACGTTGATG AGTGCGCGGA AAACATAAAC CTTTGTGAGA ATGGCCAGTG CCTGAACGTC 
CCGGGAGCTT ACCGCTGCGA ATGTGAGATG GGCTTCACCC CAGCCTCAGA CAGCAGGTCC 
TGCCAAGATA TTGACGAATG CTCCTTCCAA AATATCTGCG TCTTCGGGAC ATGCAACAAC 
TTGCCCGGGA TGTTTCACTG CATCTGTGAC GATGGTTACG GACTGGACAG AACAGGAGGA 
CACTGCACAG ATATTGACGA GTGCGCAGAT CCCATAAACT GTGTCAATGG CCTATGCGTG 
AACACACCTG GTCGCTATGA GTGTAACTGC CCGCCTGACT TTCAGCTGAA CGCCACTGGT 
GTGGGCTGCG TTGACAACCG AGTGGGCAAC TGTTACCTGA AGTTTGGTCC TCGAGGAGAT 
GGGAGTCTGT CCTGCAAGAC AGAGGTCGGG GTGGGTGTCA GTTGCTCCTC CTGCTGCTGC 
TCCCTGGGGA AGGCCTGGGG AAACCCCTGT GAGACGTGCC CCCCTGTAAA CAGCACTGAG 
TACTATAGCC TGTGTCCTGG TGGGGAAGGC TTTAGGCCGA ACCAGATCAC GATCATCTTA 
GAAGACATTG ACGAATGTCA GGAGTTACCA GGGCTCTGCC AGGGTGGAAA CTGTATCAAC 
ACTTTCGGGA GCTTCCAGTG TGAGTGTCCA CAGGGTTACT ACCTCAGTGA AGAAACCCGC 
ATCTGTGAAG ATATTGATGA GTGCTTCGCC CATCCCGGCG TGTGCGGACC TGGAACCTGC 
TACAACACTC TGGGGAATTA CACTTGCATT TGCCCGCCCG AGTACATGCA AGTCAACGGA 
GGACACAACT GCATGGACAT GAGGAAAAGC TTTTGCTACA GAAGCTATAA CGGGACCACT 
TGTGAAAACG AGTTGCCTTT TAATGTGACA AAGAGGATGT GCTGCTGCAC GTACAACGTC 
GGCAAGGCCT GGAACAAACC TTGTGAGCCA TGCCCTACGC CGGGGACAGC TGACTTTAAA 
ACTATATGTG GAAATATTCC CGGATTCACC TTTGACATTC ACACAGGAAA AGCTGTTGAC 
ATTGACGAGT GTAAAGAGAT TCCGGGCATC TGTGCAAACG GTGTGTGCAT TAACCAGATC 
GGCAGTTTCC GCTGTGAATG CCCCACAGGC TTCAGTTACA ACGACCTGCT CTTGGTCTGT 
GAAGACATCG ATGAGTGCAG CAATGGTGAC AACCTGTGCC AACGAAACGC GGACTGCATC 
AACAGCCCAG GCAGCTACCG CTGCGAGTGT GCGGCGGGCT TCAAGCTCTC CCCCAACGGG 
GCCTGTGTAG ATCGCAATGA GTGTTTGGAG ATTCCTAATG TTTGCAGTCA TGGGCTGTGT 
GTTGATTTGC AAGGAAGTTA CCAGTGCATC TGCAACAACG GCTTCAAGGC GTCTCAAGAC 
CAGACCATGT GCATGGATGT TGATGAGTGT GAGAGGCACC CATGTGGAAA CGGGACTTGT 
AAGAACACTG TCGGGTCCTA CAACTGTCTG TGCTACCCTG GCTTCGAGCT CACCCACAAC 
AACGACTGCC TAGACATAGA CGAGTGCAGC TCGTTCTTTG GCCAGGTGTG CAGAAACGGG 
CGGTGCTTCA ATGAAATCGG CTCCTTCAAA TGTCTGTGCA ACGAGGGCTA CGAACTTACT 
CCGGATGGCA AGAACTGTAT CGATACTAAT GAGTGTGTCG CACTTCCTGG CTCCTGCTCC 
CCTGGGACCT GTCAGAATTT GGAGGGATCC TTCAGGTGCA TCTGCCCCCC TGGATATGAA 
GTGAAAAGCG AAAATTGCAT TGATATAAAT GAATGTGACG AGGACCCCAA CATTTGCCTC 
TTTAGTTCCT GCACCAACAC CCCAGGGGGC TTCCAATGCA TCTGTCCCCC TGGCTTTGTC 
TTGTCTGACA ACGGACGGAG ATGCTTCGAT ACCCGCCAGA GCTTCTGCTT CACCAACTTT 
GAAAACGGAA AGTGTTCTGT ACCCAAAGCC TTCAACACCA CCAAAGCAAA ATGCTGTTGC 
AGCAAGATGC CAGGCGAGGG CTGGGGGGAC CCGTGTGAGC TGTGCCCCAA AGACGATGAA 
GTTGCCTTCC AGGATCTGTG TCCATATGGC CATGGGACAG TCCCTAGTCT TCATGACACA 
CGTGAAGATG TCAATGAGTG CCTTGAGAGC CCAGGCATTT GCTCAAACGG TCAGTGCATT 
AACACTGACG GATCCTTCCG ATGTGAGTGT CCGATGGGCT ACAATCTGGA TTACAGTGGA 
GTCCGGTGTG TGGACACTGA TGAGTGTTCG ATCGGTAATC CCTGTGGGAA CGGGACCTGC 
GCCAACGTGA TCGGGAGTTT CGAATGCAAT TGCAATGAGG GCTTTGAGCC GGGGCCCATG 
ATGAACTGTG AAGACATCAA CGAGTGTGCC CAGAACCCGC TGTTCTGCGC TTTCCGCTGC 
ATGAACACCT TTGGATCCTA TGAATGCACA TGCCCAGTTG GCTACGCCCT CAGGGAAGAC 
CAAAAGATGT GTAAAGATCT GGATGAATGC GCTGAGGGGC TTCACGACTG TGAGTCCAGA 
GGCATGATGT GTAAGAATCT GATCGGCACC TTCATGTGCA TCTGCCCCCC TGGCATGGCC 
CGCAGGCCAG ACGGAGAAGG CTGCGTAGAC GAAAACGAAT GCAGGACCAA GCCCGGCATC 
TGTGAGAATG GACGCTGCGT TAACATCATT GGAAGCTACA GGTGTGAGTG CAACGAAGGA 
TTCCAGTCAA GCTCCTCGGG CACCGAATGC CTTGACAACC GACAGGGCCT GTGCTTCGCA 
GAGGTGCTGC AGACAATGTG TCAGATGGCC TCCAGTAGCC GTAACCTCGT CACAAAGTCA 
GAATGCTGTT GTGATGGCGG GCGAGGCTGG GGCCACCAGT GTGAGCTCTG CCCACTTCCT 
GGAACTGCCC AGTACAAAAA GATCTGCCCC CATGGCCCAG GATATGCCAC TGACGGAAGA 
GATATTGACG AATGTAAGGT GATGCCAAGC CTCTGTACTA ATGGCCTGTG TGTCAACACC 
ATGGGCTCCT TCCGGTGCTT CTGCAAGGTT GGCTACACCA CAGACATCAG CGGGACAGCT 
TGTGTAGACC TGGATGAATG TTCCCAGTCT CCAAAGCCAT GCAACTTCAT CTGCAAGAAC 
ACCGAGGGCA GTTACCAGTG CTCCTGCCCA CGGGGTTACG TCCTGCAGGA AGACGGCAAG 
ACGTGCAAAG ACCTCGATGA ATGTCAAACC AAACAGCACA ACTGCCAGTT CCTCTGTGTC 
AACACCCTGG GGGGGTTCAC CTGTAAATGT CCACCCGGTT TCACCCAGCA TCACACGGCT 
TGCATTGATA ACAATGAGTG TGGGTCCCAG CCTTCCCTCT GTGGAGCAAA GGGGATCTGT 
CAGAACACTC CTGGGAGCTT CAGCTGCGAA TGCCAAAGAG GCTTCTCCCT GGATGCCTCT 
GGGCTGAACT GCGAAGATGT TGACGAATGT GACGGAAACC ACAGGTGCCA ACATGGCTGC 
CAGAACATCC TGGGGGGTTA CCGGTGCGGC TGCCCGCAGG GATATGTCCA GCACTACCAG 
TGGAACCAGT GTGTTGATGA GAATGAATGT TCCAACCCCG GTGCCTGTGG CTCGGCCTCT 
TGCTATAACA CCCTGGGGAG TTACAAGTGC GCCTGCCCCT CGCGGTTCTC CTTCGACCAG 
TTCTCCAGTG CCTGTCACGA CGTGAACGAG TGCTCCTCCT CCAAGAACCC GTGCAGCTAC 
GGCTGCTCCA ACACGGAAGG AGGCTACCTC TGTGGCTGCC CACCAGGCTA CTTCAGAGTG 
GGACAGGGCC ACTGTGTCTC GGGAATGGGC TTCAACAAAG GACAGTACCT GTCAGTGGAT 
TCGGAGGCAG AGGACGACGA AAACGCTCTG TCCCCAGAAG CATGCTACGA GTGCAAAATC 
AACGGCTACG CCAAGAAAGA TGGCCGGCGG AAGAGGAGCG CGTCAGAGCC TGAGCCCGCC 
TCAGCAGAGG AGCAAATCAG CCTAGAAAGC GTTGCCATGG ACAGCCCAGT CAACATGAAG 
TTCAACCTCT CCGGCCTCGG CTCCAAGGAG CACATCCTAG AACTGGTCCC CGCCATTGAG 
CCCCTCAACA ACCACATCCG TTACGTCATC TCCCAAGGGA ATGACGATGG TGTCTTCCGA 
ATCCATCAAA GGAATGGGCT CAGCTACCTG CACACAGCCA AGAAGAAGCT CACTCCCGGC 
ACATACACAC TGGAAATCAC CAGCATCCCT CTTTATGGGA AGAAGGAGCT AAGGAAATTG 
GAGGAACGCA ATGAGGGCAG CTACCTCCTG GGGGTGCTGG GGGAGGCTCT CAGAATGAGG 
TTGCAGATTC AGCTGTATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_114014.1
CDS	1..8721
Translation	MGRRRRLCLRPYFVWLGCVALWAQGTDGQPQPPPPKTLRPQPPPQQVRPAGASEGGFAGPEYRDEGALAASRVRRRGQQEILRGPNVCGSRFHSYCCPGWKTLPGGNQCIVPICRNSCGDGFCSRPNMCTCSSGQISPTCGGKSIQQCSVRCMNGGTCADDHCQCQKGYIGTYCGQPVCETGCQNGGRCIGPNRCACVYGFTGPQCERDYRTGPCFTQVNNQMCQGQLTGIVCTKTLCCATIGRAWGHPCEMCPAQPQPCRRGFIPNIRTGACQDVDECQAIPGLCQGGNCINTVGSFECRCPAGHKQSETTQKCEDIDECSVVPGICETGDCSNTVGSYFCLCPRGFVTSTDGSRCIDQRTGTCFSGLVNGRCAQELPGRMAKAQCCCEPGRCWGIGTIPEACPVRGSEEYRRLCLDGLPMGGIPGSSVSRPGGSGSNSNGYGPGGTGFLPIPGGNGFSPGVGGAGVGAGGQGPIITGLTILNQTIDICKHHANLCLNGRCIPTVSSYRCECNMGYKQDANGDCIDVDECTSNPCSHGDCVNTPGSYYCKCHAGFQRTPTKQACIDIDECIQNGVLCKNGRCVNTDGSFQCICNAGFELTTDGENCVGHDECTTTNMCLNGMCINEDGSFKCVCKPGFVLAPNGRCCTDVDECQTPGICMNGHCINNEGSFRCDCPPGLAVGVDGRVCVDTHMRSTCYGEIKKGVCVRPFPGAVTKYECCCANPDYGFGEPCQPCPAKNSAEFHGLCSGGVGITVDGRDINECALDPDICANGICENLRGSYRCNCNSGYEPDASGRNCIDIDECLVNRLLCDNGLCRNTPGSYSCTCPPGYVFRTETETCEDVNECESNPCVNGACRNNLGSFHCECSPGSKLSSTGLICIGSLKGTCWLNIQDNRCEVNINGATLKSECCATLGTAWGSPCERCELDAACPRGFARIKGVTCEDVNECEVFPGVCPNGRCVNNKGSFHCECPEGLTLDGTGRVCLDVRMEHCFLKWDEDECVHPVPGKFRMDACCCAVGAAWGTECEECPKPGTKEYETLCPRGPGFANRGDILTGRPFYKDINECKALPGMCTYGKCRNTIGSFKCRCNSGFALDMEERNCTDIDECRISPDLCGNGICVNTPGSFECECFEGYESGFMMMKNCMDIDECERNPLLCRGGTCVNTEGSFQCDCPLGHELSPSREDCIDINECSLSDNLCRNGKCVNMIGTYQCSCNPGYQATPDRQGCSDIDECMIMNGGCDTQCTNSEGSYECSCSEGYALMPDGRSCADIDECENNPDICDGGQCTNIPGEYRCLCYDGFMASMDMKTCIDVNECDLNPNICMFGECENTKGSFICHCQLGYSVKKGATGCTDVDECEIGAHNCDMHASCLNVPGSFKCSCREGWVGNGIKCIDLDECANGTHQCSINAQCVNTPGSYRCACSEGFTGDGFTCSDVDECAENINLCENGQCLNVPGAYRCECEMGFTPASDSRSCQDIDECSFQNICVFGTCNNLPGMFHCICDDGYGLDRTGGHCTDIDECADPINCVNGLCVNTPGRYECNCPPDFQLNATGVGCVDNRVGNCYLKFGPRGDGSLSCKTEVGVGVSCSSCCCSLGKAWGNPCETCPPVNSTEYYSLCPGGEGFRPNQITIILEDIDECQELPGLCQGGNCINTFGSFQCECPQGYYLSEETRICEDIDECFAHPGVCGPGTCYNTLGNYTCICPPEYMQVNGGHNCMDMRKSFCYRSYNGTTCENELPFNVTKRMCCCTYNVGKAWNKPCEPCPTPGTADFKTICGNIPGFTFDIHTGKAVDIDECKEIPGICANGVCINQIGSFRCECPTGFSYNDLLLVCEDIDECSNGDNLCQRNADCINSPGSYRCECAAGFKLSPNGACVDRNECLEIPNVCSHGLCVDLQGSYQCICNNGFKASQDQTMCMDVDECERHPCGNGTCKNTVGSYNCLCYPGFELTHNNDCLDIDECSSFFGQVCRNGRCFNEIGSFKCLCNEGYELTPDGKNCIDTNECVALPGSCSPGTCQNLEGSFRCICPPGYEVKSENCIDINECDEDPNICLFSSCTNTPGGFQCICPPGFVLSDNGRRCFDTRQSFCFTNFENGKCSVPKAFNTTKAKCCCSKMPGEGWGDPCELCPKDDEVAFQDLCPYGHGTVPSLHDTREDVNECLESPGICSNGQCINTDGSFRCECPMGYNLDYSGVRCVDTDECSIGNPCGNGTCANVIGSFECNCNEGFEPGPMMNCEDINECAQNPLFCAFRCMNTFGSYECTCPVGYALREDQKMCKDLDECAEGLHDCESRGMMCKNLIGTFMCICPPGMARRPDGEGCVDENECRTKPGICENGRCVNIIGSYRCECNEGFQSSSSGTECLDNRQGLCFAEVLQTMCQMASSSRNLVTKSECCCDGGRGWGHQCELCPLPGTAQYKKICPHGPGYATDGRDIDECKVMPSLCTNGLCVNTMGSFRCFCKVGYTTDISGTACVDLDECSQSPKPCNFICKNTEGSYQCSCPRGYVLQEDGKTCKDLDECQTKQHNCQFLCVNTLGGFTCKCPPGFTQHHTACIDNNECGSQPSLCGAKGICQNTPGSFSCECQRGFSLDASGLNCEDVDECDGNHRCQHGCQNILGGYRCGCPQGYVQHYQWNQCVDENECSNPGACGSASCYNTLGSYKCACPSRFSFDQFSSACHDVNECSSSKNPCSYGCSNTEGGYLCGCPPGYFRVGQGHCVSGMGFNKGQYLSVDSEAEDDENALSPEACYECKINGYAKKDGRRKRSASEPEPASAEEQISLESVAMDSPVNMKFNLSGLGSKEHILELVPAIEPLNNHIRYVISQGNDDGVFRIHQRNGLSYLHTAKKKLTPGTYTLEITSIPLYGKKELRKLEERNEGSYLLGVLGEALRMRLQIQLY

Target ORF information:

RefSeq Version	NM_031826.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus fibrillin 2 (Fbn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031826.1

ORF Insert Sequence:

ATGGGGAGAA GACGGAGGCT GTGTCTCCGG CCCTACTTCG TGTGGCTGGG TTGCGTGGCG 
CTCTGGGCGC AGGGCACAGA TGGCCAGCCC CAGCCTCCTC CACCAAAGAC GCTCCGGCCC 
CAGCCGCCCC CGCAACAGGT CCGGCCCGCA GGAGCGTCCG AAGGTGGCTT CGCGGGGCCT 
GAGTACAGGG ACGAGGGCGC GTTAGCCGCG AGCCGGGTCC GCAGGCGAGG ACAGCAGGAA 
ATACTCCGAG GGCCCAACGT GTGCGGTTCT AGATTCCACT CCTATTGCTG CCCGGGATGG 
AAGACGCTCC CAGGAGGAAA TCAGTGCATT GTCCCCATTT GCAGGAACAG CTGTGGAGAT 
GGATTCTGTT CCCGCCCCAA CATGTGTACC TGTTCCAGCG GACAAATATC CCCGACCTGT 
GGAGGCAAAT CAATTCAGCA GTGTAGTGTG AGGTGCATGA ACGGTGGGAC CTGTGCGGAT 
GACCACTGTC AGTGCCAGAA GGGATACATC GGGACCTACT GTGGACAACC TGTCTGTGAG 
ACTGGATGCC AGAATGGTGG GCGTTGCATC GGCCCTAACC GCTGTGCTTG TGTTTATGGA 
TTCACCGGGC CACAGTGCGA AAGAGATTAC AGGACAGGCC CATGTTTCAC TCAAGTCAAT 
AATCAGATGT GCCAGGGGCA GCTGACAGGC ATCGTCTGTA CGAAGACGCT GTGCTGTGCC 
ACCATTGGGC GAGCCTGGGG CCATCCTTGT GAGATGTGCC CAGCCCAGCC TCAGCCCTGC 
CGCCGGGGCT TCATCCCCAA CATCCGCACT GGAGCATGTC AGGATGTTGA CGAATGCCAG 
GCGATTCCAG GATTGTGCCA GGGCGGAAAC TGTATCAACA CAGTGGGCTC TTTTGAATGC 
AGATGCCCTG CTGGCCACAA GCAGAGTGAA ACCACACAGA AATGTGAAGA CATCGACGAG 
TGCAGCGTGG TTCCTGGAAT CTGTGAGACA GGCGACTGTT CCAACACCGT GGGAAGCTAT 
TTTTGTCTCT GTCCACGCGG CTTCGTTACC TCAACAGATG GCTCCCGGTG CATTGATCAG 
AGAACTGGCA CATGCTTCTC CGGCCTGGTG AATGGCCGCT GTGCTCAAGA ACTCCCAGGC 
AGAATGGCAA AAGCTCAGTG CTGTTGCGAG CCCGGCCGTT GCTGGGGCAT CGGGACCATT 
CCTGAGGCCT GTCCTGTCAG AGGTTCTGAA GAGTACCGTA GACTGTGCCT GGATGGTCTT 
CCAATGGGCG GTATTCCGGG GAGTTCAGTT TCTAGACCTG GAGGGTCTGG GAGCAACAGC 
AATGGTTATG GCCCAGGAGG AACAGGCTTC CTGCCCATCC CTGGAGGCAA TGGCTTTTCT 
CCTGGTGTTG GAGGAGCTGG AGTAGGAGCT GGAGGGCAGG GACCCATCAT CACGGGACTA 
ACAATTCTCA ACCAGACAAT AGACATCTGT AAGCACCATG CCAATCTGTG TTTGAACGGA 
CGTTGCATAC CTACTGTTTC CAGCTACAGA TGCGAATGTA ACATGGGCTA CAAGCAGGAT 
GCTAATGGAG ATTGTATAGA TGTTGATGAA TGTACATCAA ACCCCTGCAG TCATGGAGAT 
TGTGTTAACA CACCTGGATC CTATTACTGT AAATGTCACG CTGGATTCCA GAGGACTCCT 
ACGAAGCAAG CATGTATCGA CATTGATGAG TGCATCCAGA ATGGGGTTCT GTGTAAAAAT 
GGCCGATGCG TGAACACAGA TGGCAGCTTC CAGTGCATTT GCAATGCTGG CTTTGAGTTA 
ACCACAGATG GAGAAAACTG TGTTGGCCAC GATGAATGTA CAACGACCAA CATGTGCTTG 
AACGGGATGT GTATCAATGA AGATGGCAGC TTCAAGTGCG TGTGCAAGCC AGGCTTTGTC 
CTGGCTCCAA ATGGCCGCTG CTGCACAGAC GTGGACGAGT GCCAGACCCC GGGCATCTGC 
ATGAATGGGC ACTGCATCAA CAACGAAGGG TCCTTCCGCT GTGACTGTCC TCCCGGCCTG 
GCTGTGGGTG TGGATGGACG TGTATGTGTT GACACGCATA TGCGTAGCAC CTGCTATGGG 
GAAATCAAGA AAGGAGTGTG TGTGCGGCCA TTCCCGGGTG CAGTGACCAA GTATGAATGC 
TGCTGTGCTA ATCCAGACTA TGGTTTTGGA GAGCCCTGCC AGCCTTGTCC TGCTAAAAAT 
TCAGCGGAGT TCCATGGTCT TTGTAGCGGT GGCGTAGGCA TCACTGTGGA TGGAAGAGAT 
ATCAATGAGT GTGCTTTGGA TCCTGACATA TGTGCCAACG GGATCTGTGA AAACTTGCGA 
GGCAGCTACC GGTGTAACTG CAACAGTGGC TATGAGCCAG ACGCCTCAGG AAGAAATTGC 
ATTGACATTG ATGAGTGCTT GGTAAACAGG CTGCTTTGTG ACAATGGCCT ATGCAGAAAT 
ACGCCTGGCA GTTACAGCTG CACGTGTCCC CCTGGCTATG TGTTCCGGAC AGAGACAGAG 
ACCTGCGAAG ATGTCAATGA GTGTGAAAGC AACCCGTGTG TCAATGGAGC CTGCAGGAAC 
AACCTGGGCT CTTTCCACTG CGAGTGCTCG CCTGGGAGCA AGCTCAGCTC CACGGGACTG 
ATCTGCATCG GCAGCCTGAA AGGGACCTGC TGGCTCAACA TCCAGGACAA CCGCTGCGAA 
GTGAACATTA ACGGAGCCAC CCTGAAATCT GAATGCTGCG CCACGCTTGG AACTGCCTGG 
GGGAGCCCCT GTGAGCGCTG CGAGCTAGAT GCAGCTTGCC CGAGAGGGTT CGCCAGGATC 
AAAGGTGTGA CCTGCGAAGA CGTTAATGAG TGCGAGGTGT TTCCTGGCGT CTGTCCAAAT 
GGACGCTGTG TCAACAACAA AGGCTCTTTC CACTGTGAAT GCCCTGAAGG CCTTACTCTG 
GATGGCACAG GCCGGGTGTG TCTGGATGTC CGCATGGAGC ACTGCTTCCT GAAGTGGGAT 
GAGGATGAGT GCGTCCACCC CGTCCCCGGG AAGTTCCGCA TGGACGCCTG CTGCTGTGCG 
GTCGGGGCGG CTTGGGGCAC AGAGTGTGAA GAGTGTCCAA AACCTGGCAC CAAGGAATAT 
GAGACCCTGT GTCCCCGGGG CCCCGGCTTT GCTAACAGAG GGGACATCCT CACTGGGCGT 
CCATTTTACA AAGATATCAA CGAGTGCAAG GCCTTGCCTG GGATGTGCAC CTATGGGAAG 
TGTAGAAACA CAATCGGAAG CTTCAAGTGT CGTTGCAACA GTGGCTTTGC CCTGGACATG 
GAGGAGAGGA ACTGTACTGA CATTGATGAG TGCAGGATCT CCCCTGACCT CTGTGGCAAT 
GGCATCTGTG TCAACACGCC AGGCAGCTTT GAGTGCGAGT GCTTTGAGGG CTACGAGAGC 
GGCTTCATGA TGATGAAGAA TTGCATGGAC ATTGACGAAT GTGAACGTAA CCCTCTCCTT 
TGTAGGGGTG GCACCTGTGT GAACACTGAG GGAAGCTTTC AGTGTGACTG CCCACTGGGA 
CACGAGCTGT CGCCGTCACG CGAGGACTGC ATAGATATTA ACGAGTGCTC CCTGAGCGAC 
AATCTCTGCA GAAATGGCAA ATGTGTGAAC ATGATCGGAA CCTACCAGTG TTCTTGCAAT 
CCTGGATACC AGGCCACGCC AGATCGCCAG GGCTGTTCAG ATATCGATGA GTGTATGATA 
ATGAACGGGG GCTGCGACAC CCAGTGCACC AACTCAGAAG GAAGCTACGA ATGCAGCTGC 
AGCGAAGGGT ACGCACTGAT GCCAGACGGA CGGTCGTGCG CAGATATTGA CGAGTGTGAA 
AACAACCCCG ACATCTGTGA CGGCGGCCAG TGTACCAACA TTCCGGGGGA GTATCGCTGC 
CTCTGCTACG ATGGCTTCAT GGCGTCGATG GACATGAAGA CATGCATCGA TGTGAACGAG 
TGTGACCTGA ATCCCAACAT CTGCATGTTT GGGGAGTGTG AGAACACAAA GGGCTCCTTC 
ATTTGCCACT GCCAGCTGGG CTACTCAGTG AAGAAGGGGG CCACGGGATG CACAGACGTG 
GACGAGTGTG AAATTGGCGC CCACAACTGT GACATGCACG CCTCGTGCCT GAATGTCCCT 
GGGAGCTTCA AATGCAGCTG CAGAGAAGGC TGGGTGGGAA ATGGCATCAA ATGCATTGAT 
CTGGATGAAT GCGCGAATGG AACCCACCAG TGCAGCATAA ACGCCCAGTG TGTCAACACC 
CCAGGCTCGT ACCGATGTGC CTGCTCGGAG GGTTTCACGG GAGACGGCTT TACTTGCTCA 
GACGTTGATG AGTGCGCGGA AAACATAAAC CTTTGTGAGA ATGGCCAGTG CCTGAACGTC 
CCGGGAGCTT ACCGCTGCGA ATGTGAGATG GGCTTCACCC CAGCCTCAGA CAGCAGGTCC 
TGCCAAGATA TTGACGAATG CTCCTTCCAA AATATCTGCG TCTTCGGGAC ATGCAACAAC 
TTGCCCGGGA TGTTTCACTG CATCTGTGAC GATGGTTACG GACTGGACAG AACAGGAGGA 
CACTGCACAG ATATTGACGA GTGCGCAGAT CCCATAAACT GTGTCAATGG CCTATGCGTG 
AACACACCTG GTCGCTATGA GTGTAACTGC CCGCCTGACT TTCAGCTGAA CGCCACTGGT 
GTGGGCTGCG TTGACAACCG AGTGGGCAAC TGTTACCTGA AGTTTGGTCC TCGAGGAGAT 
GGGAGTCTGT CCTGCAAGAC AGAGGTCGGG GTGGGTGTCA GTTGCTCCTC CTGCTGCTGC 
TCCCTGGGGA AGGCCTGGGG AAACCCCTGT GAGACGTGCC CCCCTGTAAA CAGCACTGAG 
TACTATAGCC TGTGTCCTGG TGGGGAAGGC TTTAGGCCGA ACCAGATCAC GATCATCTTA 
GAAGACATTG ACGAATGTCA GGAGTTACCA GGGCTCTGCC AGGGTGGAAA CTGTATCAAC 
ACTTTCGGGA GCTTCCAGTG TGAGTGTCCA CAGGGTTACT ACCTCAGTGA AGAAACCCGC 
ATCTGTGAAG ATATTGATGA GTGCTTCGCC CATCCCGGCG TGTGCGGACC TGGAACCTGC 
TACAACACTC TGGGGAATTA CACTTGCATT TGCCCGCCCG AGTACATGCA AGTCAACGGA 
GGACACAACT GCATGGACAT GAGGAAAAGC TTTTGCTACA GAAGCTATAA CGGGACCACT 
TGTGAAAACG AGTTGCCTTT TAATGTGACA AAGAGGATGT GCTGCTGCAC GTACAACGTC 
GGCAAGGCCT GGAACAAACC TTGTGAGCCA TGCCCTACGC CGGGGACAGC TGACTTTAAA 
ACTATATGTG GAAATATTCC CGGATTCACC TTTGACATTC ACACAGGAAA AGCTGTTGAC 
ATTGACGAGT GTAAAGAGAT TCCGGGCATC TGTGCAAACG GTGTGTGCAT TAACCAGATC 
GGCAGTTTCC GCTGTGAATG CCCCACAGGC TTCAGTTACA ACGACCTGCT CTTGGTCTGT 
GAAGACATCG ATGAGTGCAG CAATGGTGAC AACCTGTGCC AACGAAACGC GGACTGCATC 
AACAGCCCAG GCAGCTACCG CTGCGAGTGT GCGGCGGGCT TCAAGCTCTC CCCCAACGGG 
GCCTGTGTAG ATCGCAATGA GTGTTTGGAG ATTCCTAATG TTTGCAGTCA TGGGCTGTGT 
GTTGATTTGC AAGGAAGTTA CCAGTGCATC TGCAACAACG GCTTCAAGGC GTCTCAAGAC 
CAGACCATGT GCATGGATGT TGATGAGTGT GAGAGGCACC CATGTGGAAA CGGGACTTGT 
AAGAACACTG TCGGGTCCTA CAACTGTCTG TGCTACCCTG GCTTCGAGCT CACCCACAAC 
AACGACTGCC TAGACATAGA CGAGTGCAGC TCGTTCTTTG GCCAGGTGTG CAGAAACGGG 
CGGTGCTTCA ATGAAATCGG CTCCTTCAAA TGTCTGTGCA ACGAGGGCTA CGAACTTACT 
CCGGATGGCA AGAACTGTAT CGATACTAAT GAGTGTGTCG CACTTCCTGG CTCCTGCTCC 
CCTGGGACCT GTCAGAATTT GGAGGGATCC TTCAGGTGCA TCTGCCCCCC TGGATATGAA 
GTGAAAAGCG AAAATTGCAT TGATATAAAT GAATGTGACG AGGACCCCAA CATTTGCCTC 
TTTAGTTCCT GCACCAACAC CCCAGGGGGC TTCCAATGCA TCTGTCCCCC TGGCTTTGTC 
TTGTCTGACA ACGGACGGAG ATGCTTCGAT ACCCGCCAGA GCTTCTGCTT CACCAACTTT 
GAAAACGGAA AGTGTTCTGT ACCCAAAGCC TTCAACACCA CCAAAGCAAA ATGCTGTTGC 
AGCAAGATGC CAGGCGAGGG CTGGGGGGAC CCGTGTGAGC TGTGCCCCAA AGACGATGAA 
GTTGCCTTCC AGGATCTGTG TCCATATGGC CATGGGACAG TCCCTAGTCT TCATGACACA 
CGTGAAGATG TCAATGAGTG CCTTGAGAGC CCAGGCATTT GCTCAAACGG TCAGTGCATT 
AACACTGACG GATCCTTCCG ATGTGAGTGT CCGATGGGCT ACAATCTGGA TTACAGTGGA 
GTCCGGTGTG TGGACACTGA TGAGTGTTCG ATCGGTAATC CCTGTGGGAA CGGGACCTGC 
GCCAACGTGA TCGGGAGTTT CGAATGCAAT TGCAATGAGG GCTTTGAGCC GGGGCCCATG 
ATGAACTGTG AAGACATCAA CGAGTGTGCC CAGAACCCGC TGTTCTGCGC TTTCCGCTGC 
ATGAACACCT TTGGATCCTA TGAATGCACA TGCCCAGTTG GCTACGCCCT CAGGGAAGAC 
CAAAAGATGT GTAAAGATCT GGATGAATGC GCTGAGGGGC TTCACGACTG TGAGTCCAGA 
GGCATGATGT GTAAGAATCT GATCGGCACC TTCATGTGCA TCTGCCCCCC TGGCATGGCC 
CGCAGGCCAG ACGGAGAAGG CTGCGTAGAC GAAAACGAAT GCAGGACCAA GCCCGGCATC 
TGTGAGAATG GACGCTGCGT TAACATCATT GGAAGCTACA GGTGTGAGTG CAACGAAGGA 
TTCCAGTCAA GCTCCTCGGG CACCGAATGC CTTGACAACC GACAGGGCCT GTGCTTCGCA 
GAGGTGCTGC AGACAATGTG TCAGATGGCC TCCAGTAGCC GTAACCTCGT CACAAAGTCA 
GAATGCTGTT GTGATGGCGG GCGAGGCTGG GGCCACCAGT GTGAGCTCTG CCCACTTCCT 
GGAACTGCCC AGTACAAAAA GATCTGCCCC CATGGCCCAG GATATGCCAC TGACGGAAGA 
GATATTGACG AATGTAAGGT GATGCCAAGC CTCTGTACTA ATGGCCTGTG TGTCAACACC 
ATGGGCTCCT TCCGGTGCTT CTGCAAGGTT GGCTACACCA CAGACATCAG CGGGACAGCT 
TGTGTAGACC TGGATGAATG TTCCCAGTCT CCAAAGCCAT GCAACTTCAT CTGCAAGAAC 
ACCGAGGGCA GTTACCAGTG CTCCTGCCCA CGGGGTTACG TCCTGCAGGA AGACGGCAAG 
ACGTGCAAAG ACCTCGATGA ATGTCAAACC AAACAGCACA ACTGCCAGTT CCTCTGTGTC 
AACACCCTGG GGGGGTTCAC CTGTAAATGT CCACCCGGTT TCACCCAGCA TCACACGGCT 
TGCATTGATA ACAATGAGTG TGGGTCCCAG CCTTCCCTCT GTGGAGCAAA GGGGATCTGT 
CAGAACACTC CTGGGAGCTT CAGCTGCGAA TGCCAAAGAG GCTTCTCCCT GGATGCCTCT 
GGGCTGAACT GCGAAGATGT TGACGAATGT GACGGAAACC ACAGGTGCCA ACATGGCTGC 
CAGAACATCC TGGGGGGTTA CCGGTGCGGC TGCCCGCAGG GATATGTCCA GCACTACCAG 
TGGAACCAGT GTGTTGATGA GAATGAATGT TCCAACCCCG GTGCCTGTGG CTCGGCCTCT 
TGCTATAACA CCCTGGGGAG TTACAAGTGC GCCTGCCCCT CGCGGTTCTC CTTCGACCAG 
TTCTCCAGTG CCTGTCACGA CGTGAACGAG TGCTCCTCCT CCAAGAACCC GTGCAGCTAC 
GGCTGCTCCA ACACGGAAGG AGGCTACCTC TGTGGCTGCC CACCAGGCTA CTTCAGAGTG 
GGACAGGGCC ACTGTGTCTC GGGAATGGGC TTCAACAAAG GACAGTACCT GTCAGTGGAT 
TCGGAGGCAG AGGACGACGA AAACGCTCTG TCCCCAGAAG CATGCTACGA GTGCAAAATC 
AACGGCTACG CCAAGAAAGA TGGCCGGCGG AAGAGGAGCG CGTCAGAGCC TGAGCCCGCC 
TCAGCAGAGG AGCAAATCAG CCTAGAAAGC GTTGCCATGG ACAGCCCAGT CAACATGAAG 
TTCAACCTCT CCGGCCTCGG CTCCAAGGAG CACATCCTAG AACTGGTCCC CGCCATTGAG 
CCCCTCAACA ACCACATCCG TTACGTCATC TCCCAAGGGA ATGACGATGG TGTCTTCCGA 
ATCCATCAAA GGAATGGGCT CAGCTACCTG CACACAGCCA AGAAGAAGCT CACTCCCGGC 
ACATACACAC TGGAAATCAC CAGCATCCCT CTTTATGGGA AGAAGGAGCT AAGGAAATTG 
GAGGAACGCA ATGAGGGCAG CTACCTCCTG GGGGTGCTGG GGGAGGCTCT CAGAATGAGG 
TTGCAGATTC AGCTGTATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Disruption of murine Adamtsl4 results in zonular fiber detachment from the lens and in retinal pigment epithelium dedifferentiation.
Human molecular genetics24(24)6958-74(2015 Dec)
Collin GB,Hubmacher D,Charette JR,Hicks WL,Stone L,Yu M,Naggert JK,Krebs MP,Peachey NS,Apte SS,Nishina PM

Human eye development is characterized by coordinated expression of fibrillin isoforms.
Investigative ophthalmology & visual science55(12)7934-44(2014 Nov)
Hubmacher D,Reinhardt DP,Plesec T,Schenke-Layland K,Apte SS

Rare variants in FBN1 and FBN2 are associated with severe adolescent idiopathic scoliosis.
Human molecular genetics23(19)5271-82(2014 Oct)
Buchan JG,Alvarado DM,Haller GE,Cruchaga C,Harms MB,Zhang T,Willing MC,Grange DK,Braverman AC,Miller NH,Morcuende JA,Tang NL,Lam TP,Ng BK,Cheng JC,Dobbs MB,Gurnett CA

Comparative proteomic analysis of supportive and unsupportive extracellular matrix substrates for human embryonic stem cell maintenance.
The Journal of biological chemistry288(26)18716-31(2013 Jun)
Soteriou D,Iskender B,Byron A,Humphries JD,Borg-Bartolo S,Haddock MC,Baxter MA,Knight D,Humphries MJ,Kimber SJ

A trans-acting protein effect causes severe eye malformation in the Mp mouse.
PLoS genetics9(12)e1003998(2013)
Rainger J,Keighren M,Keene DR,Charbonneau NL,Rainger JK,Fisher M,Mella S,Huang JT,Rose L,van't Hof R,Sakai LY,Jackson IJ,Fitzpatrick DR

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Fbn2 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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