Eif4a3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eif4a3
Entrez Gene ID	688288
Full Name	eukaryotic translation initiation factor 4A3
Synonyms	eIF4A-III
General protein information	Preferred Names eukaryotic translation initiation factor 4A3 Names eukaryotic initiation factor 4A-III ATP-dependent RNA helicase DDX48 ATP-dependent RNA helicase eIF4A-3 DEAD box protein 48 eIF-4A-III eukaryotic translation initiation factor 4A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001100158.2	NP_001093628.1	eukaryotic initiation factor 4A-III

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	EIF4A3	XP_533130.2
Bos taurus (cattle)	EIF4A3	NP_001039653.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003089	XP_312776.3
Caenorhabditis elegans (roundworm)	F33D11.10	NP_491703.1
Saccharomyces cerevisiae (baker's yeast)	FAL1	NP_010304.3
Neurospora crassa	NCU01234	XP_961600.1
Arabidopsis thaliana (thale cress)	EIF4A-III	NP_188610.1
Gallus gallus (chicken)	EIF4A3	NP_001025820.1
Danio rerio (zebrafish)	eif4a3	NP_957372.1
Drosophila melanogaster (fruit fly)	eIF4AIII	NP_649788.2
Caenorhabditis elegans (roundworm)	Y65B4A.6	NP_490761.2
Oryza sativa (rice)	Os01g0639100	NP_001043673.1
Homo sapiens (human)	EIF4A3	NP_055555.1
	EIF4A3	XP_001110130.1
Mus musculus (house mouse)	Eif4a3	NP_619610.1
Rattus norvegicus (Norway rat)	Eif4a3	NP_001093628.1
Schizosaccharomyces pombe (fission yeast)	SPAC1F5.10	NP_592863.1
Oryza sativa (rice)	Os03g0566800	NP_001050506.1
Xenopus tropicalis (tropical clawed frog)	eif4a3	NP_001107349.1
Pan troglodytes (chimpanzee)	EIF4A3	XP_511724.3
Kluyveromyces lactis	KLLA0A10659g	XP_451466.1
Eremothecium gossypii	AGOS_AER408W	NP_985263.2
Magnaporthe oryzae (rice blast fungus)	MGG_04885	XP_003712368.1

Gene Ontology
Bibliography

Related articles in PubMed

Structure of a human catalytic step I spliceosome.
Zhan X, Yan C, Zhang X, Lei J, Shi Y
Science (New York, N.Y.)(2018 Jan)

A noncoding expansion in EIF4A3 causes Richieri-Costa-Pereira syndrome, a craniofacial disorder associated with limb defects.
Favaro FP, Alvizi L, Zechi-Ceide RM, Bertola D, Felix TM, de Souza J, Raskin S, Twigg SR, Weiner AM, Armas P, Margarit E, Calcaterra NB, Andersen GR, McGowan SJ, Wilkie AO, Richieri-Costa A, de Almeida ML, Passos-Bueno MR
American journal of human genetics94(1)120-8(2014 Jan)

Contribution of copy number variants involving nonsense-mediated mRNA decay pathway genes to neuro-developmental disorders.
Nguyen LS, Kim HG, Rosenfeld JA, Shen Y, Gusella JF, Lacassie Y, Layman LC, Shaffer LG, G?cz J
Human molecular genetics22(9)1816-25(2013 May)

Expression of the core exon-junction complex factor eukaryotic initiation factor 4A3 is increased during spatial exploration and striatally-mediated learning.
Barker-Haliski ML, Pastuzyn ED, Keefe KA
Neuroscience22651-61(2012 Dec)

Human CWC22 escorts the helicase eIF4AIII to spliceosomes and promotes exon junction complex assembly.
Barbosa I, Haque N, Fiorini F, Barrandon C, Tomasetto C, Blanchette M, Le Hir H
Nature structural & molecular biology19(10)983-90(2012 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings thus further implicate eIF4A3 as a key mediator of Arc protein mRNA availability underlying learning and memory processes in vivo
Title: Expression of the core exon-junction complex factor eukaryotic initiation factor 4A3 is increased during spatial exploration and striatally-mediated learning.

Eukaryotic initiation factor 4a3 is a selenium-regulated RNA-binding protein that selectively inhibits selenocysteine incorporation.
Title: Eukaryotic initiation factor 4a3 is a selenium-regulated RNA-binding protein that selectively inhibits selenocysteine incorporation.

Data show that eIF4AIII, a core exon junction complex (EJC) component loaded onto mRNAs by pre-mRNA splicing, is associated with neuronal mRNA granules and dendritic mRNAs.
Title: The EJC factor eIF4AIII modulates synaptic strength and neuronal protein expression.

The following Eif4a3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eif4a3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10727	NM_001100158.2 Latest version!	Rattus norvegicus eukaryotic translation initiation factor 4A3 (Eif4a3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eif4a3 ( NM_001100158.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001093628.1 Rattus norvegicus eukaryotic translation initiation factor 4A3 (Eif4a3), mRNA.

Price & Availability↑

CloneID	ORa10727
Clone ID Related Accession (Same CDS sequence)	NM_001100158.2
Accession Version	NM_001100158.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	eukaryotic initiation factor 4A-III
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CO559861.1 and BC105875.1. On Jan 22, 2011 this sequence version replaced NM_001100158.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC105875.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCCA CGGCCACGAT GGCGACGTCG GGCTCCGCGC GGAAGCGGCT GCTCAAAGAG 
GAGGACATGA CCAAAGTGGA GTTCGAGACC AGCGAGGAGG TGGACGTGAC CCCCACGTTC 
GACACCATGG GCCTTCGGGA GGACCTGCTG CGCGGCATCT ACGCCTACGG TTTTGAAAAA 
CCTTCTGCGA TCCAACAGCG TGCTATCAAG CAGATAATTA AAGGGAGAGA TGTCATTGCA 
CAGTCTCAGT CTGGCACAGG CAAGACGGCC ACCTTCAGTA TTTCAGTCCT TCAGTGCTTG 
GATATCCAGG TTCGAGAAAC CCAAGCTTTG ATATTGGCTC CAACAAGAGA GTTAGCAGTG 
CAGATTCAGA AGGGTCTGCT TGCTTTGGGG GATTACATGA ACGTGCAGTG CCATGCCTGC 
ATTGGTGGCA CCAATGTCGG GGAGGACATC CGGAAGCTGG ACTATGGGCA GCATGTTGTG 
GCGGGCACAC CAGGACGAGT CTTTGATATG ATCCGCCGTA GAAGTTTAAG GACACGGGCA 
ATCAAGATGT TGGTTTTGGA TGAAGCTGAT GAAATGTTGA ACAAAGGTTT CAAGGAGCAG 
ATCTATGACG TGTACAGGTA CTTGCCACCA GCCACTCAGG TTGTTCTCAT CAGCGCCACG 
CTGCCCCATG AGATCCTGGA GATGACCAAC AAGTTCATGA CCGACCCCAT CCGCATCTTG 
GTGAAGCGTG ATGAGTTGAC TCTGGAAGGC ATCAAACAGT TCTTCGTGGC CGTGGAAAGA 
GAGGAATGGA AGTTCGACAC TCTCTGTGAT CTCTACGACA CGCTGACCAT CACCCAGGCT 
GTCATCTTCT GCAACACCAA GAGGAAGGTT GACTGGCTGA CAGAGAAAAT GAGAGAAGCC 
AATTTCACAG TGTCGTCCAT GCACGGAGAC ATGCCCCAGA AAGAGCGTGA GTCCATCATG 
AAGGAGTTCC GGTCAGGTGC CAGCCGAGTG CTCATCTCCA CAGATGTCTG GGCGCGGGGC 
CTCGATGTCC CTCAGGTGTC CCTCATCATT AACTATGACC TGCCCAACAA CAGAGAACTG 
TACATTCACA GAATCGGGAG ATCAGGTCGA TATGGACGCA AAGGTGTGGC CATCAATTTT 
GTGAAGAATG ATGACATCCG CATCCTCAGG GACATTGAGC AGTACTACTC CACCCAGATA 
GATGAGATGC CTATGAATGT GGCTGACCTC ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093628.1
CDS	61..1296
Translation	MAATATMATSGSARKRLLKEEDMTKVEFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFSISVLQCLDIQVRETQALILAPTRELAVQIQKGLLALGDYMNVQCHACIGGTNVGEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRAIKMLVLDEADEMLNKGFKEQIYDVYRYLPPATQVVLISATLPHEILEMTNKFMTDPIRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMREANFTVSSMHGDMPQKERESIMKEFRSGASRVLISTDVWARGLDVPQVSLIINYDLPNNRELYIHRIGRSGRYGRKGVAINFVKNDDIRILRDIEQYYSTQIDEMPMNVADLI

Target ORF information:

RefSeq Version	NM_001100158.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus eukaryotic translation initiation factor 4A3 (Eif4a3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100158.2

ORF Insert Sequence:

ATGGCGGCCA CGGCCACGAT GGCGACGTCG GGCTCCGCGC GGAAGCGGCT GCTCAAAGAG 
GAGGACATGA CCAAAGTGGA GTTCGAGACC AGCGAGGAGG TGGACGTGAC CCCCACGTTC 
GACACCATGG GCCTTCGGGA GGACCTGCTG CGCGGCATCT ACGCCTACGG TTTTGAAAAA 
CCTTCTGCGA TCCAACAGCG TGCTATCAAG CAGATAATTA AAGGGAGAGA TGTCATTGCA 
CAGTCTCAGT CTGGCACAGG CAAGACGGCC ACCTTCAGTA TTTCAGTCCT TCAGTGCTTG 
GATATCCAGG TTCGAGAAAC CCAAGCTTTG ATATTGGCTC CAACAAGAGA GTTAGCAGTG 
CAGATTCAGA AGGGTCTGCT TGCTTTGGGG GATTACATGA ACGTGCAGTG CCATGCCTGC 
ATTGGTGGCA CCAATGTCGG GGAGGACATC CGGAAGCTGG ACTATGGGCA GCATGTTGTG 
GCGGGCACAC CAGGACGAGT CTTTGATATG ATCCGCCGTA GAAGTTTAAG GACACGGGCA 
ATCAAGATGT TGGTTTTGGA TGAAGCTGAT GAAATGTTGA ACAAAGGTTT CAAGGAGCAG 
ATCTATGACG TGTACAGGTA CTTGCCACCA GCCACTCAGG TTGTTCTCAT CAGCGCCACG 
CTGCCCCATG AGATCCTGGA GATGACCAAC AAGTTCATGA CCGACCCCAT CCGCATCTTG 
GTGAAGCGTG ATGAGTTGAC TCTGGAAGGC ATCAAACAGT TCTTCGTGGC CGTGGAAAGA 
GAGGAATGGA AGTTCGACAC TCTCTGTGAT CTCTACGACA CGCTGACCAT CACCCAGGCT 
GTCATCTTCT GCAACACCAA GAGGAAGGTT GACTGGCTGA CAGAGAAAAT GAGAGAAGCC 
AATTTCACAG TGTCGTCCAT GCACGGAGAC ATGCCCCAGA AAGAGCGTGA GTCCATCATG 
AAGGAGTTCC GGTCAGGTGC CAGCCGAGTG CTCATCTCCA CAGATGTCTG GGCGCGGGGC 
CTCGATGTCC CTCAGGTGTC CCTCATCATT AACTATGACC TGCCCAACAA CAGAGAACTG 
TACATTCACA GAATCGGGAG ATCAGGTCGA TATGGACGCA AAGGTGTGGC CATCAATTTT 
GTGAAGAATG ATGACATCCG CATCCTCAGG GACATTGAGC AGTACTACTC CACCCAGATA 
GATGAGATGC CTATGAATGT GGCTGACCTC ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structure of a human catalytic step I spliceosome.
Science (New York, N.Y.)(2018 Jan)
Zhan X,Yan C,Zhang X,Lei J,Shi Y

A noncoding expansion in EIF4A3 causes Richieri-Costa-Pereira syndrome, a craniofacial disorder associated with limb defects.
American journal of human genetics94(1)120-8(2014 Jan)
Favaro FP,Alvizi L,Zechi-Ceide RM,Bertola D,Felix TM,de Souza J,Raskin S,Twigg SR,Weiner AM,Armas P,Margarit E,Calcaterra NB,Andersen GR,McGowan SJ,Wilkie AO,Richieri-Costa A,de Almeida ML,Passos-Bueno MR

Contribution of copy number variants involving nonsense-mediated mRNA decay pathway genes to neuro-developmental disorders.
Human molecular genetics22(9)1816-25(2013 May)
Nguyen LS,Kim HG,Rosenfeld JA,Shen Y,Gusella JF,Lacassie Y,Layman LC,Shaffer LG,G?cz J

Expression of the core exon-junction complex factor eukaryotic initiation factor 4A3 is increased during spatial exploration and striatally-mediated learning.
Neuroscience22651-61(2012 Dec)
Barker-Haliski ML,Pastuzyn ED,Keefe KA

Human CWC22 escorts the helicase eIF4AIII to spliceosomes and promotes exon junction complex assembly.
Nature structural & molecular biology19(10)983-90(2012 Oct)
Barbosa I,Haque N,Fiorini F,Barrandon C,Tomasetto C,Blanchette M,Le Hir H