U2af1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	U2af1
Entrez Gene ID	687575
Full Name	U2 small nuclear RNA auxiliary factor 1
General protein information	Preferred Names U2 small nuclear RNA auxiliary factor 1 Names splicing factor U2AF 35 kDa subunit
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20p12

mRNA and Protein(s)

mRNA	Protein	Name
XM_006256228.3	XP_006256290.1	splicing factor U2AF 35 kDa subunit isoform X1
NM_001044291.1	NP_001037756.1	splicing factor U2AF 35 kDa subunit
XM_017601774.1	XP_017457263.1	splicing factor U2AF 35 kDa subunit isoform X2

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	SPAP8A3.06	NP_594945.1
Pan troglodytes (chimpanzee)	U2AF1	XP_003949235.1
Canis lupus familiaris (dog)	U2AF1	XP_003640135.1
Bos taurus (cattle)	U2AF1	NP_001073737.1
Rattus norvegicus (Norway rat)	U2af1	NP_001037756.1
Drosophila melanogaster (fruit fly)	U2af38	NP_477208.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002956	XP_311943.3
Magnaporthe oryzae (rice blast fungus)	MGG_09948	XP_003710052.1
Oryza sativa (rice)	Os01g0667800	NP_001043811.2
Mus musculus (house mouse)	U2af1	NP_077149.2
Caenorhabditis elegans (roundworm)	uaf-2	NP_503036.1
Xenopus tropicalis (tropical clawed frog)	u2af1	NP_001039113.1
	LOC722383	XP_001118538.2
Gallus gallus (chicken)	U2AF1	NP_989986.2
Neurospora crassa	NCU03261	XP_964414.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome-wide RNAi screen identifies the Parkinson disease GWAS risk locus SREBF1 as a regulator of mitophagy.
Ivatt RM, Sanchez-Martinez A, Godena VK, Brown S, Ziviani E, Whitworth AJ
Proceedings of the National Academy of Sciences of the United States of America111(23)8494-9(2014 Jun)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

The spliceosome: design principles of a dynamic RNP machine.
Wahl MC, Will CL, L?hrmann R
Cell136(4)701-18(2009 Feb)

PHF5A represents a bridge protein between splicing proteins and ATP-dependent helicases and is differentially expressed during mouse spermatogenesis.
Rzymski T, Grzmil P, Meinhardt A, Wolf S, Burfeind P
Cytogenetic and genome research121(3-4)232-44(2008)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following U2af1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the U2af1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12888	NM_001044291.1 Latest version!	Rattus norvegicus U2 small nuclear RNA auxiliary factor 1 (U2af1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa39320	XM_006256228.3 Latest version!	Rattus norvegicus U2 small nuclear RNA auxiliary factor 1 (U2af1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa60189	XM_017601774.1 Latest version!	Rattus norvegicus U2 small nuclear RNA auxiliary factor 1 (U2af1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

U2af1 ( NM_001044291.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001037756.1 Rattus norvegicus U2 small nuclear RNA auxiliary factor 1 (U2af1), mRNA.

Price & Availability↑

CloneID	ORa12888
Clone ID Related Accession (Same CDS sequence)	NM_001044291.1
Accession Version	NM_001044291.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	splicing factor U2AF 35 kDa subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC105905.1. On Aug 24, 2006 this sequence version replaced XM_001079206.1. ##Evidence-Data-START## Transcript exon combination :: BC105905.1, CD372826.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906347, SAMN01906349 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGAAT ACTTGGCCTC CATCTTCGGC ACCGAGAAAG ACAAAGTCAA CTGTTCATTT 
TATTTCAAAA TTGGAGCGTG TCGTCATGGA GACAGATGTT CTCGGTTGCA CAATAAACCG 
ACCTTTAGCC AGACCATTGC CCTCTTGAAC ATTTACCGTA ACCCTCAAAA CTCTTCCCAG 
TCTGCTGACG GTTTGCGCTG TGCGGTGAGC GACGTGGAGA TGCAGGAGCA CTATGACGAG 
TTCTTTGAGG AAGTCTTCAC TGAGATGGAA GAGAAGTACG GGGAGGTCGA GGAGATGAAC 
GTCTGTGACA ACCTAGGAGA CCACCTGGTG GGGAATGTGT ATGTCAAGTT CCGACGTGAG 
GAGGATGCGG AGAAAGCAGT GATCGACTTG AACAACCGCT GGTTTAATGG GCAGCCAATC 
CACGCAGAGC TGTCCCCAGT AACTGACTTC AGGGAAGCTT GCTGCCGCCA GTATGAAATG 
GGGGAGTGCA CAAGAGGGGG CTTCTGCAAC TTCATGCATC TGAAGCCGAT CTCAAGAGAG 
CTGCGACGGG AGCTGTATGG GCGCCGGCGC AAGAAGCATA GATCCAGGTC CCGATCCAGG 
GAGCGGCGTT CCCGATCGAG AGACCGTGGA CGCGGTGGTG GAGGTGGAGG TGGAGGCGGT 
GGAGGACGTG AGCGTGACAG GAGGCGGTCA AGAGACCGGG AGAGATCTGG GCGATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001037756.1
CDS	52..771
Translation	MAEYLASIFGTEKDKVNCSFYFKIGACRHGDRCSRLHNKPTFSQTIALLNIYRNPQNSSQSADGLRCAVSDVEMQEHYDEFFEEVFTEMEEKYGEVEEMNVCDNLGDHLVGNVYVKFRREEDAEKAVIDLNNRWFNGQPIHAELSPVTDFREACCRQYEMGECTRGGFCNFMHLKPISRELRRELYGRRRKKHRSRSRSRERRSRSRDRGRGGGGGGGGGGGRERDRRRSRDRERSGRF

Target ORF information:

RefSeq Version	NM_001044291.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus U2 small nuclear RNA auxiliary factor 1 (U2af1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044291.1

ORF Insert Sequence:

ATGGCGGAAT ACTTGGCCTC CATCTTCGGC ACCGAGAAAG ACAAAGTCAA CTGTTCATTT 
TATTTCAAAA TTGGAGCGTG TCGTCATGGA GACAGATGTT CTCGGTTGCA CAATAAACCG 
ACCTTTAGCC AGACCATTGC CCTCTTGAAC ATTTACCGTA ACCCTCAAAA CTCTTCCCAG 
TCTGCTGACG GTTTGCGCTG TGCGGTGAGC GACGTGGAGA TGCAGGAGCA CTATGACGAG 
TTCTTTGAGG AAGTCTTCAC TGAGATGGAA GAGAAGTACG GGGAGGTCGA GGAGATGAAC 
GTCTGTGACA ACCTAGGAGA CCACCTGGTG GGGAATGTGT ATGTCAAGTT CCGACGTGAG 
GAGGATGCGG AGAAAGCAGT GATCGACTTG AACAACCGCT GGTTTAATGG GCAGCCAATC 
CACGCAGAGC TGTCCCCAGT AACTGACTTC AGGGAAGCTT GCTGCCGCCA GTATGAAATG 
GGGGAGTGCA CAAGAGGGGG CTTCTGCAAC TTCATGCATC TGAAGCCGAT CTCAAGAGAG 
CTGCGACGGG AGCTGTATGG GCGCCGGCGC AAGAAGCATA GATCCAGGTC CCGATCCAGG 
GAGCGGCGTT CCCGATCGAG AGACCGTGGA CGCGGTGGTG GAGGTGGAGG TGGAGGCGGT 
GGAGGACGTG AGCGTGACAG GAGGCGGTCA AGAGACCGGG AGAGATCTGG GCGATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

U2af1 ( XM_006256228.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256290.1 Rattus norvegicus U2 small nuclear RNA auxiliary factor 1 (U2af1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa39320
Clone ID Related Accession (Same CDS sequence)	XM_006256228.3
Accession Version	XM_006256228.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	splicing factor U2AF 35 kDa subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256228.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAAT ACTTGGCCTC CATCTTCGGC ACCGAGAAAG ACAAAGTCAA CTGTTCATTT 
TATTTCAAAA TTGGAGCGTG TCGTCATGGA GACAGATGTT CTCGGTTGCA CAATAAACCG 
ACCTTTAGCC AGACCATCTT GATTCAAAAC ATCTATCGTA ATCCCCAAAA CAGTGCACAG 
ACGGCTGACG GCTCACACTG TGCGGTGAGC GACGTGGAGA TGCAGGAGCA CTATGACGAG 
TTCTTTGAGG AAGTCTTCAC TGAGATGGAA GAGAAGTACG GGGAGGTCGA GGAGATGAAC 
GTCTGTGACA ACCTAGGAGA CCACCTGGTG GGGAATGTGT ATGTCAAGTT CCGACGTGAG 
GAGGATGCGG AGAAAGCAGT GATCGACTTG AACAACCGCT GGTTTAATGG GCAGCCAATC 
CACGCAGAGC TGTCCCCAGT AACTGACTTC AGGGAAGCTT GCTGCCGCCA GTATGAAATG 
GGGGAGTGCA CAAGAGGGGG CTTCTGCAAC TTCATGCATC TGAAGCCGAT CTCAAGAGAG 
CTGCGACGGG AGCTGTATGG GCGCCGGCGC AAGAAGCATA GATCCAGGTC CCGATCCAGG 
GAGCGGCGTT CCCGATCGAG AGACCGTGGA CGCGGTGGTG GAGGTGGAGG TGGAGGCGGT 
GGAGGACGTG AGCGTGACAG GAGGCGGTCA AGAGACCGGG AGAGATCTGG GCGATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256290.1
CDS	76..795
Translation	MAEYLASIFGTEKDKVNCSFYFKIGACRHGDRCSRLHNKPTFSQTILIQNIYRNPQNSAQTADGSHCAVSDVEMQEHYDEFFEEVFTEMEEKYGEVEEMNVCDNLGDHLVGNVYVKFRREEDAEKAVIDLNNRWFNGQPIHAELSPVTDFREACCRQYEMGECTRGGFCNFMHLKPISRELRRELYGRRRKKHRSRSRSRERRSRSRDRGRGGGGGGGGGGGRERDRRRSRDRERSGRF

Target ORF information:

RefSeq Version	XM_006256228.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus U2 small nuclear RNA auxiliary factor 1 (U2af1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256228.3

ORF Insert Sequence:

ATGGCGGAAT ACTTGGCCTC CATCTTCGGC ACCGAGAAAG ACAAAGTCAA CTGTTCATTT 
TATTTCAAAA TTGGAGCGTG TCGTCATGGA GACAGATGTT CTCGGTTGCA CAATAAACCG 
ACCTTTAGCC AGACCATCTT GATTCAAAAC ATCTATCGTA ATCCCCAAAA CAGTGCACAG 
ACGGCTGACG GCTCACACTG TGCGGTGAGC GACGTGGAGA TGCAGGAGCA CTATGACGAG 
TTCTTTGAGG AAGTCTTCAC TGAGATGGAA GAGAAGTACG GGGAGGTCGA GGAGATGAAC 
GTCTGTGACA ACCTAGGAGA CCACCTGGTG GGGAATGTGT ATGTCAAGTT CCGACGTGAG 
GAGGATGCGG AGAAAGCAGT GATCGACTTG AACAACCGCT GGTTTAATGG GCAGCCAATC 
CACGCAGAGC TGTCCCCAGT AACTGACTTC AGGGAAGCTT GCTGCCGCCA GTATGAAATG 
GGGGAGTGCA CAAGAGGGGG CTTCTGCAAC TTCATGCATC TGAAGCCGAT CTCAAGAGAG 
CTGCGACGGG AGCTGTATGG GCGCCGGCGC AAGAAGCATA GATCCAGGTC CCGATCCAGG 
GAGCGGCGTT CCCGATCGAG AGACCGTGGA CGCGGTGGTG GAGGTGGAGG TGGAGGCGGT 
GGAGGACGTG AGCGTGACAG GAGGCGGTCA AGAGACCGGG AGAGATCTGG GCGATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

U2af1 ( XM_017601774.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017457263.1 Rattus norvegicus U2 small nuclear RNA auxiliary factor 1 (U2af1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa60189
Clone ID Related Accession (Same CDS sequence)	XM_017601774.1
Accession Version	XM_017601774.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	splicing factor U2AF 35 kDa subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGAGC ACTATGACGA GTTCTTTGAG GAAGTCTTCA CTGAGATGGA AGAGAAGTAC 
GGGGAGGTCG AGGAGATGAA CGTCTGTGAC AACCTAGGAG ACCACCTGGT GGGGAATGTG 
TATGTCAAGT TCCGACGTGA GGAGGATGCG GAGAAAGCAG TGATCGACTT GAACAACCGC 
TGGTTTAATG GGCAGCCAAT CCACGCAGAG CTGTCCCCAG TAACTGACTT CAGGGAAGCT 
TGCTGCCGCC AGTATGAAAT GGGGGAGTGC ACAAGAGGGG GCTTCTGCAA CTTCATGCAT 
CTGAAGCCGA TCTCAAGAGA GCTGCGACGG GAGCTGTATG GGCGCCGGCG CAAGAAGCAT 
AGATCCAGGT CCCGATCCAG GGAGCGGCGT TCCCGATCGA GAGACCGTGG ACGCGGTGGT 
GGAGGTGGAG GTGGAGGCGG TGGAGGACGT GAGCGTGACA GGAGGCGGTC AAGAGACCGG 
GAGAGATCTG GGCGATTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017457263.1
CDS	155..655
Translation	MQEHYDEFFEEVFTEMEEKYGEVEEMNVCDNLGDHLVGNVYVKFRREEDAEKAVIDLNNRWFNGQPIHAELSPVTDFREACCRQYEMGECTRGGFCNFMHLKPISRELRRELYGRRRKKHRSRSRSRERRSRSRDRGRGGGGGGGGGGGRERDRRRSRDRERSGRF

Target ORF information:

RefSeq Version	XM_017601774.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus U2 small nuclear RNA auxiliary factor 1 (U2af1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601774.1

ORF Insert Sequence:

ATGCAGGAGC ACTATGACGA GTTCTTTGAG GAAGTCTTCA CTGAGATGGA AGAGAAGTAC 
GGGGAGGTCG AGGAGATGAA CGTCTGTGAC AACCTAGGAG ACCACCTGGT GGGGAATGTG 
TATGTCAAGT TCCGACGTGA GGAGGATGCG GAGAAAGCAG TGATCGACTT GAACAACCGC 
TGGTTTAATG GGCAGCCAAT CCACGCAGAG CTGTCCCCAG TAACTGACTT CAGGGAAGCT 
TGCTGCCGCC AGTATGAAAT GGGGGAGTGC ACAAGAGGGG GCTTCTGCAA CTTCATGCAT 
CTGAAGCCGA TCTCAAGAGA GCTGCGACGG GAGCTGTATG GGCGCCGGCG CAAGAAGCAT 
AGATCCAGGT CCCGATCCAG GGAGCGGCGT TCCCGATCGA GAGACCGTGG ACGCGGTGGT 
GGAGGTGGAG GTGGAGGCGG TGGAGGACGT GAGCGTGACA GGAGGCGGTC AAGAGACCGG 
GAGAGATCTG GGCGATTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-wide RNAi screen identifies the Parkinson disease GWAS risk locus SREBF1 as a regulator of mitophagy.
Proceedings of the National Academy of Sciences of the United States of America111(23)8494-9(2014 Jun)
Ivatt RM,Sanchez-Martinez A,Godena VK,Brown S,Ziviani E,Whitworth AJ

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

The spliceosome: design principles of a dynamic RNP machine.
Cell136(4)701-18(2009 Feb)
Wahl MC,Will CL,L?hrmann R

PHF5A represents a bridge protein between splicing proteins and ATP-dependent helicases and is differentially expressed during mouse spermatogenesis.
Cytogenetic and genome research121(3-4)232-44(2008)
Rzymski T,Grzmil P,Meinhardt A,Wolf S,Burfeind P