LOC684545 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC684545
Entrez Gene ID	684545
Full Name	NLR family pyrin domain containing 2
General protein information	Preferred Names NLR family pyrin domain containing 2 Names NACHT, LRR and PYD domains-containing protein 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q12

mRNA and Protein(s)

mRNA	Protein	Name
XM_001070923.3	XP_001070923.1	NACHT, LRR and PYD domains-containing protein 2 isoform X2
XM_017590431.1	XP_017445920.1	NACHT, LRR and PYD domains-containing protein 2 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	LOC684545	XP_003748813.1
Mus musculus (house mouse)	Nlrp2	NP_808358.2
Homo sapiens (human)	NLRP2	NP_001167552.1
	NLRP2	NP_001107824.1
Pan troglodytes (chimpanzee)	NLRP2	XP_001175085.1
Canis lupus familiaris (dog)	NLRP2	XP_541420.4
Bos taurus (cattle)	NLRP2	XP_002695462.3

Gene Ontology

The following LOC684545 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC684545 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa57057	XM_017590431.1 Latest version!	Rattus norvegicus similar to NACHT, leucine rich repeat and PYD containing 2 (LOC684545), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa14216	XM_001070923.3 Latest version!	Rattus norvegicus similar to NACHT, leucine rich repeat and PYD containing 2 (LOC684545), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC684545 ( XM_017590431.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017445920.1 Rattus norvegicus similar to NACHT, leucine rich repeat and PYD containing 2 (LOC684545), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa57057
Clone ID Related Accession (Same CDS sequence)	XM_017590431.1
Accession Version	XM_017590431.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NACHT, LRR and PYD domains-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGAACATT CTGATCAACT GGGTTTCAAC CTAAAAGATG TCCTGAAAAA GCTAGACAAG 
ACCGAGTTGA ACAATTTCAA GAGTACATTG AAGAGCTGTT CTCTACCACA GACAATGAAG 
CAGATCTCCA AGCTGACAAT GGACCTGGCC AATGGAGCTC AACTAGCAGA AATTCTCACA 
GACTACTGCC CCAATGATTG GGTAAAGAGA GTTACCTTCA AGATCTTAGA GGAAATAAAC 
CGTGCAGATC TAGCTGAGTT AGTAATGAAG CAAGTCGAAG AAGCAGCATC AAAAGCATCG 
GAAGAAAAGG TTTCTTCTAA ACCAATATCC CCCCCAATAA TTGATGGTAA AGAGCACAAA 
CATAAAAAGG AATGGAGGAA TATGTACACT GAAAAATGGA AGAAGAACTT CTGGCCCAAA 
TGCAATAAAG AAATCTATGT GGATACTGAG AGCTACAGGA CACTCCTGAC ATTGTGTAAC 
CCGAAGATAG AAATGCCATT TGCTTATACA ATTGTGCTGC ATGGCCCTCC AGGCTCTGGA 
AAAACCACAA TGGCAAGGAG GCTAATGCTA GAATGGTCAG AGAGTAAGCA GGCCCAGAGT 
TTTTCATGTG CCTTCTACAT CAGCTGCAGG AAACTTAATA ATAGTAAACC TTGCACTTTT 
GCCCACCTCC TCTCCATGAA CAATCCCTCC TGGAGATACT ATATGATTCG GCGTCTAGTC 
CTGTCAGAAC AATTCTTATT TGTGGTAGAT GGTTTTGATG AGCTGACATT CCCAGCAGGA 
GCTCTGCTCC GTGATATCTG TGGTGACTGG AACACAGTGA AGCCTGTAGA GGTCTTACTG 
AGCAGTTTGC TAATGAGGAA GATGGAACCT CATAGCACAC TGCTGGTAAC CACACAAACA 
CAGTCTTTGC ACCAAATCTA CCTCATGGTA GATCAGCCTG TCCTAGTAGA AACTCAGGGT 
TTCTTAGAGC CAGAAAAACA GGAGTACTTT CAGCAATACT TTGAAGATGA GGAGGGTGAG 
GAAGAGGGTG AAGGTGAGGG AAAGGCACTG AGAGCTCTTA AGGAAGTTAA ATGCAATGAA 
GCTCTCTTCC AAATGGCCTC ACTTCCTGCT GCATGTGGAA TATTCTGCCT CTGTCTGGAG 
CTAGGAATGA AGAAGGGGGA AGACCTGGCT CTGACATGTC AGACCTACAC CTCTATGTTC 
CTGAACTTCC TCTGCAGAGT CTTCCTACAA GAAGCCTGTG ATGGCCATCT CAACAAGCAA 
CTCCAAATCT TATTCAAGAA AATATGTATC CTGGCTGCCG ATAGTCTCTT AGAACAGGTG 
CCCATACTCT GTGAAGAAGA CTTCTTGAAA TTAAAATTGA ACCTGAATAA ATTGCATCCC 
GTGGTGTACA GGTGCATCTT TCTTGAGGAC AGTTCCTGTA CAAACTGTCT TTCTTTCATC 
TGCCTCAGTA TCCAGCAGCT CCTGGCTGCC ATAATATTTG TTCAGGAACT CGGGCAAGAA 
AGCAAGGATG TCTCTAAATA TAGTATACAG AAGATGCTTT CTAGGGAAGC AAGGTTGAAA 
AACCCCAACC TGTCTGGAGT GCTGCCATTT GTATTTAGCC TCCTGAACAA AACTCATATT 
CAAGAGCTGG AGACCATTTT TGGCTGCCAA ATATCAATGG GGGTCAAGAG AAAATTCCTG 
GAGTGTGAGT TGGGGGAAAA TAAACCCTTC CTATTACTGA TGGATCTGCA AGAGATCTTT 
TCCTGTCTCT ATGAATCTCA GGAGGAGGAG TTTGTGAAAG AAGCCATGGC CCTCTTTGGG 
GAAGTCTCCT TGCATTTGAA AACCAACATG GACCTCGTCC ATGCTTCATT CTGCCTCAAG 
AACTCTCAGA ACTTGCAGAC AATGTTCCTT AAAGTAGAAA AGGCAGTCTT CCCAGAGAAT 
TTTGCTACAC TGGAGTCCAC AGCTGAGGAC CAAAGGTTCC AGGACAAAAA ATGCATGCTA 
ACTCTCTGGA CAGACTTTTG TGACACATTT CATTCAAATG AGAAACTGGC CTTTCTAGAA 
ATCCACGAAA GCTTCCTCAA CAGCTCCTCA CTGGAGATTC TTTGTGAAAA GCTGTCCTCT 
GCTCCCTGCC ATCTCCAGAA AGTGGTCCTC AAAAATATTT CCCCAGATGA TGCGTATGAG 
AAATTATGCC TCATTTTTTG TGGTTATGAA ACCATCTCAC ATCTGACTTT GCAAGGTGGT 
AAACTGGATA GCATGCTTCA CTCACTGTGT AAGGTTGTGA AAAATCCAGC ATGCAACCTG 
AAGTTTCTCA GCCTGGGATC TTGTTCTACC ACTGCTCAGA AATGGGATGA CTTCTTCCCA 
GCCCTGAAAG CCAATCAATC CCTGATAAGT CTGGATCTCA CAGACAATAA TCTCTTGGAC 
AAGGGTGTGA GGCTTCTATG TAATACTTGG AAGCAATCAA AGTGCATACT GCAGAGGGTA 
TCGTTGGAGA ACTGTCATCT CACTGAAGTC TGTTGCAAGG ACCTCTCTTC TGTTCTGATG 
GTCAGCCAGA CTCTAACACA CCTTAGCCTG GCCAAGAATG AACTTGGAGA TAATGGAGTG 
AAAAAACTAT GTGAAAGCTT AAGCTTCCCC AAATGCAAAC TACAGACCCT GGTTCTGTGG 
TCCTGTAACA TCACCAGCAA TGGTTGCCAC TATCTCTCAA AGATGCTCGG ACAAGCACCA 
AGCCTGAAGC ACTTGGATCT GGGGCTAAAT CGCATTGGAA CCACAGGTGC AAAGTTTTTG 
TGTGAGGCTT TGAAGAGCCC CAGGAGTAAC TTGAAAAGTT TGTGGCTGTG TGGATGCTCC 
ATCATTCCAA CGAACTGCAA AGACTTCTCT GAGATTCTCA GAAGCAACAA GAGCCTGAAC 
ACTCTAGACC TGTCTCAGAA CGTCATGGGA ACTGATGCAG TAAAGATATT CTGTGAATCC 
TTGAAACTCC AAATTTGTCC CCTCCAAACA CTCAGGTTGA AATTTGATGA AGCTAACCCC 
AGTATCCAGA AGCTGATCCA AGAAATGAAG GAGAGCCATC CACAATTGAG CATTACCAGT 
GACCAAGTGG ATCCTAAGAA AAAGAGTCAT TCTTTCCCTC ATTTCATTTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017445920.1
CDS	34..3147
Translation	MEHSDQLGFNLKDVLKKLDKTELNNFKSTLKSCSLPQTMKQISKLTMDLANGAQLAEILTDYCPNDWVKRVTFKILEEINRADLAELVMKQVEEAASKASEEKVSSKPISPPIIDGKEHKHKKEWRNMYTEKWKKNFWPKCNKEIYVDTESYRTLLTLCNPKIEMPFAYTIVLHGPPGSGKTTMARRLMLEWSESKQAQSFSCAFYISCRKLNNSKPCTFAHLLSMNNPSWRYYMIRRLVLSEQFLFVVDGFDELTFPAGALLRDICGDWNTVKPVEVLLSSLLMRKMEPHSTLLVTTQTQSLHQIYLMVDQPVLVETQGFLEPEKQEYFQQYFEDEEGEEEGEGEGKALRALKEVKCNEALFQMASLPAACGIFCLCLELGMKKGEDLALTCQTYTSMFLNFLCRVFLQEACDGHLNKQLQILFKKICILAADSLLEQVPILCEEDFLKLKLNLNKLHPVVYRCIFLEDSSCTNCLSFICLSIQQLLAAIIFVQELGQESKDVSKYSIQKMLSREARLKNPNLSGVLPFVFSLLNKTHIQELETIFGCQISMGVKRKFLECELGENKPFLLLMDLQEIFSCLYESQEEEFVKEAMALFGEVSLHLKTNMDLVHASFCLKNSQNLQTMFLKVEKAVFPENFATLESTAEDQRFQDKKCMLTLWTDFCDTFHSNEKLAFLEIHESFLNSSSLEILCEKLSSAPCHLQKVVLKNISPDDAYEKLCLIFCGYETISHLTLQGGKLDSMLHSLCKVVKNPACNLKFLSLGSCSTTAQKWDDFFPALKANQSLISLDLTDNNLLDKGVRLLCNTWKQSKCILQRVSLENCHLTEVCCKDLSSVLMVSQTLTHLSLAKNELGDNGVKKLCESLSFPKCKLQTLVLWSCNITSNGCHYLSKMLGQAPSLKHLDLGLNRIGTTGAKFLCEALKSPRSNLKSLWLCGCSIIPTNCKDFSEILRSNKSLNTLDLSQNVMGTDAVKIFCESLKLQICPLQTLRLKFDEANPSIQKLIQEMKESHPQLSITSDQVDPKKKSHSFPHFIF

Target ORF information:

RefSeq Version	XM_017590431.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus similar to NACHT, leucine rich repeat and PYD containing 2 (LOC684545), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017590431.1

ORF Insert Sequence:

ATGGAACATT CTGATCAACT GGGTTTCAAC CTAAAAGATG TCCTGAAAAA GCTAGACAAG 
ACCGAGTTGA ACAATTTCAA GAGTACATTG AAGAGCTGTT CTCTACCACA GACAATGAAG 
CAGATCTCCA AGCTGACAAT GGACCTGGCC AATGGAGCTC AACTAGCAGA AATTCTCACA 
GACTACTGCC CCAATGATTG GGTAAAGAGA GTTACCTTCA AGATCTTAGA GGAAATAAAC 
CGTGCAGATC TAGCTGAGTT AGTAATGAAG CAAGTCGAAG AAGCAGCATC AAAAGCATCG 
GAAGAAAAGG TTTCTTCTAA ACCAATATCC CCCCCAATAA TTGATGGTAA AGAGCACAAA 
CATAAAAAGG AATGGAGGAA TATGTACACT GAAAAATGGA AGAAGAACTT CTGGCCCAAA 
TGCAATAAAG AAATCTATGT GGATACTGAG AGCTACAGGA CACTCCTGAC ATTGTGTAAC 
CCGAAGATAG AAATGCCATT TGCTTATACA ATTGTGCTGC ATGGCCCTCC AGGCTCTGGA 
AAAACCACAA TGGCAAGGAG GCTAATGCTA GAATGGTCAG AGAGTAAGCA GGCCCAGAGT 
TTTTCATGTG CCTTCTACAT CAGCTGCAGG AAACTTAATA ATAGTAAACC TTGCACTTTT 
GCCCACCTCC TCTCCATGAA CAATCCCTCC TGGAGATACT ATATGATTCG GCGTCTAGTC 
CTGTCAGAAC AATTCTTATT TGTGGTAGAT GGTTTTGATG AGCTGACATT CCCAGCAGGA 
GCTCTGCTCC GTGATATCTG TGGTGACTGG AACACAGTGA AGCCTGTAGA GGTCTTACTG 
AGCAGTTTGC TAATGAGGAA GATGGAACCT CATAGCACAC TGCTGGTAAC CACACAAACA 
CAGTCTTTGC ACCAAATCTA CCTCATGGTA GATCAGCCTG TCCTAGTAGA AACTCAGGGT 
TTCTTAGAGC CAGAAAAACA GGAGTACTTT CAGCAATACT TTGAAGATGA GGAGGGTGAG 
GAAGAGGGTG AAGGTGAGGG AAAGGCACTG AGAGCTCTTA AGGAAGTTAA ATGCAATGAA 
GCTCTCTTCC AAATGGCCTC ACTTCCTGCT GCATGTGGAA TATTCTGCCT CTGTCTGGAG 
CTAGGAATGA AGAAGGGGGA AGACCTGGCT CTGACATGTC AGACCTACAC CTCTATGTTC 
CTGAACTTCC TCTGCAGAGT CTTCCTACAA GAAGCCTGTG ATGGCCATCT CAACAAGCAA 
CTCCAAATCT TATTCAAGAA AATATGTATC CTGGCTGCCG ATAGTCTCTT AGAACAGGTG 
CCCATACTCT GTGAAGAAGA CTTCTTGAAA TTAAAATTGA ACCTGAATAA ATTGCATCCC 
GTGGTGTACA GGTGCATCTT TCTTGAGGAC AGTTCCTGTA CAAACTGTCT TTCTTTCATC 
TGCCTCAGTA TCCAGCAGCT CCTGGCTGCC ATAATATTTG TTCAGGAACT CGGGCAAGAA 
AGCAAGGATG TCTCTAAATA TAGTATACAG AAGATGCTTT CTAGGGAAGC AAGGTTGAAA 
AACCCCAACC TGTCTGGAGT GCTGCCATTT GTATTTAGCC TCCTGAACAA AACTCATATT 
CAAGAGCTGG AGACCATTTT TGGCTGCCAA ATATCAATGG GGGTCAAGAG AAAATTCCTG 
GAGTGTGAGT TGGGGGAAAA TAAACCCTTC CTATTACTGA TGGATCTGCA AGAGATCTTT 
TCCTGTCTCT ATGAATCTCA GGAGGAGGAG TTTGTGAAAG AAGCCATGGC CCTCTTTGGG 
GAAGTCTCCT TGCATTTGAA AACCAACATG GACCTCGTCC ATGCTTCATT CTGCCTCAAG 
AACTCTCAGA ACTTGCAGAC AATGTTCCTT AAAGTAGAAA AGGCAGTCTT CCCAGAGAAT 
TTTGCTACAC TGGAGTCCAC AGCTGAGGAC CAAAGGTTCC AGGACAAAAA ATGCATGCTA 
ACTCTCTGGA CAGACTTTTG TGACACATTT CATTCAAATG AGAAACTGGC CTTTCTAGAA 
ATCCACGAAA GCTTCCTCAA CAGCTCCTCA CTGGAGATTC TTTGTGAAAA GCTGTCCTCT 
GCTCCCTGCC ATCTCCAGAA AGTGGTCCTC AAAAATATTT CCCCAGATGA TGCGTATGAG 
AAATTATGCC TCATTTTTTG TGGTTATGAA ACCATCTCAC ATCTGACTTT GCAAGGTGGT 
AAACTGGATA GCATGCTTCA CTCACTGTGT AAGGTTGTGA AAAATCCAGC ATGCAACCTG 
AAGTTTCTCA GCCTGGGATC TTGTTCTACC ACTGCTCAGA AATGGGATGA CTTCTTCCCA 
GCCCTGAAAG CCAATCAATC CCTGATAAGT CTGGATCTCA CAGACAATAA TCTCTTGGAC 
AAGGGTGTGA GGCTTCTATG TAATACTTGG AAGCAATCAA AGTGCATACT GCAGAGGGTA 
TCGTTGGAGA ACTGTCATCT CACTGAAGTC TGTTGCAAGG ACCTCTCTTC TGTTCTGATG 
GTCAGCCAGA CTCTAACACA CCTTAGCCTG GCCAAGAATG AACTTGGAGA TAATGGAGTG 
AAAAAACTAT GTGAAAGCTT AAGCTTCCCC AAATGCAAAC TACAGACCCT GGTTCTGTGG 
TCCTGTAACA TCACCAGCAA TGGTTGCCAC TATCTCTCAA AGATGCTCGG ACAAGCACCA 
AGCCTGAAGC ACTTGGATCT GGGGCTAAAT CGCATTGGAA CCACAGGTGC AAAGTTTTTG 
TGTGAGGCTT TGAAGAGCCC CAGGAGTAAC TTGAAAAGTT TGTGGCTGTG TGGATGCTCC 
ATCATTCCAA CGAACTGCAA AGACTTCTCT GAGATTCTCA GAAGCAACAA GAGCCTGAAC 
ACTCTAGACC TGTCTCAGAA CGTCATGGGA ACTGATGCAG TAAAGATATT CTGTGAATCC 
TTGAAACTCC AAATTTGTCC CCTCCAAACA CTCAGGTTGA AATTTGATGA AGCTAACCCC 
AGTATCCAGA AGCTGATCCA AGAAATGAAG GAGAGCCATC CACAATTGAG CATTACCAGT 
GACCAAGTGG ATCCTAAGAA AAAGAGTCAT TCTTTCCCTC ATTTCATTTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC684545 ( XM_001070923.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_001070923.1 Rattus norvegicus similar to NACHT, leucine rich repeat and PYD containing 2 (LOC684545), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa14216
Clone ID Related Accession (Same CDS sequence)	XM_001070923.3
Accession Version	XM_001070923.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NACHT, LRR and PYD domains-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000069.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_001070923.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACATT CTGATCAACT GGGTTTCAAC CTAAAAGATG TCCTGAAAAA GCTAGACAAG 
ACCGAGTTGA ACAATTTCAA GAGTACATTG AAGAGCTGTT CTCTACCACA GACAATGAAG 
CAGATCTCCA AGCTGACAAT GGACCTGGCC AATGGAGCTC AACTAGCAGA AATTCTCACA 
GACTACTGCC CCAATGATTG GGTAAAGAGA GTTACCTTCA AGATCTTAGA GGAAATAAAC 
CGTGCAGATC TAGCTGAGTT AGTAATGAAG CAAGTCGAAG AAGCAGCATC AAAAGCATCG 
GAAGAAAAGG TTTCTTCTAA ACCAAGAGAA CCCTTGAAGA CATCACTTTT CCCACAGGAT 
TTCGTTCAAG GAGATGAAAG ACCAGAGCAC AAACATAAAA AGGAATGGAG GAATATGTAC 
ACTGAAAAAT GGAAGAAGAA CTTCTGGCCC AAATGCAATA AAGAAATCTA TGTGGATACT 
GAGAGCTACA GGACACTCCT GACATTGTGT AACCCGAAGA TAGAAATGCC ATTTGCTTAT 
ACAATTGTGC TGCATGGCCC TCCAGGCTCT GGAAAAACCA CAATGGCAAG GAGGCTAATG 
CTAGAATGGT CAGAGAGTAA GCAGGCCCAG AGTTTTTCAT GTGCCTTCTA CATCAGCTGC 
AGGAAACTTA ATAATAGTAA ACCTTGCACT TTTGCCCACC TCCTCTCCAT GAACAATCCC 
TCCTGGAGAT ACTATATGAT TCGGCGTCTA GTCCTGTCAG AACAATTCTT ATTTGTGGTA 
GATGGTTTTG ATGAGCTGAC ATTCCCAGCA GGAGCTCTGC TCCGTGATAT CTGTGGTGAC 
TGGAACACAG TGAAGCCTGT AGAGGTCTTA CTGAGCAGTT TGCTAATGAG GAAGATGGAA 
CCTCATAGCA CACTGCTGGT AACCACACAA ACACAGTCTT TGCACCAAAT CTACCTCATG 
GTAGATCAGC CTGTCCTAGT AGAAACTCAG GGTTTCTTAG AGCCAGAAAA ACAGGAGTAC 
TTTCAGCAAT ACTTTGAAGA TGAGGAGGGT GAGGAAGAGG GTGAAGGTGA GGGAAAGGCA 
CTGAGAGCTC TTAAGGAAGT TAAATGCAAT GAAGCTCTCT TCCAAATGGC CTCACTTCCT 
GCTGCATGTG GAATATTCTG CCTCTGTCTG GAGCTAGGAA TGAAGAAGGG GGAAGACCTG 
GCTCTGACAT GTCAGACCTA CACCTCTATG TTCCTGAACT TCCTCTGCAG AGTCTTCCTA 
CAAGAAGCCT GTGATGGCCA TCTCAACAAG CAACTCCAAA TCTTATTCAA GAAAATATGT 
ATCCTGGCTG CCGATAGTCT CTTAGAACAG GTGCCCATAC TCTGTGAAGA AGACTTCTTG 
AAATTAAAAT TGAACCTGAA TAAATTGCAT CCCGTGGTGT ACAGGTGCAT CTTTCTTGAG 
GACAGTTCCT GTACAAACTG TCTTTCTTTC ATCTGCCTCA GTATCCAGCA GCTCCTGGCT 
GCCATAATAT TTGTTCAGGA ACTCGGGCAA GAAAGCAAGG ATGTCTCTAA ATATAGTATA 
CAGAAGATGC TTTCTAGGGA AGCAAGGTTG AAAAACCCCA ACCTGTCTGG AGTGCTGCCA 
TTTGTATTTA GCCTCCTGAA CAAAACTCAT ATTCAAGAGC TGGAGACCAT TTTTGGCTGC 
CAAATATCAA TGGGGGTCAA GAGAAAATTC CTGGAGTGTG AGTTGGGGGA AAATAAACCC 
TTCCTATTAC TGATGGATCT GCAAGAGATC TTTTCCTGTC TCTATGAATC TCAGGAGGAG 
GAGTTTGTGA AAGAAGCCAT GGCCCTCTTT GGGGAAGTCT CCTTGCATTT GAAAACCAAC 
ATGGACCTCG TCCATGCTTC ATTCTGCCTC AAGAACTCTC AGAACTTGCA GACAATGTTC 
CTTAAAGTAG AAAAGGCAGT CTTCCCAGAG AATTTTGCTA CACTGGAGTC CACAGCTGAG 
GACCAAAGGT TCCAGGACAA AAAATGCATG CTAACTCTCT GGACAGACTT TTGTGACACA 
TTTCATTCAA ATGAGAAACT GGCCTTTCTA GAAATCCACG AAAGCTTCCT CAACAGCTCC 
TCACTGGAGA TTCTTTGTGA AAAGCTGTCC TCTGCTCCCT GCCATCTCCA GAAAGTGGTC 
CTCAAAAATA TTTCCCCAGA TGATGCGTAT GAGAAATTAT GCCTCATTTT TTGTGGTTAT 
GAAACCATCT CACATCTGAC TTTGCAAGGT GGTAAACTGG ATAGCATGCT TCACTCACTG 
TGTAAGGTTG TGAAAAATCC AGCATGCAAC CTGAAGTTTC TCAGCCTGGG ATCTTGTTCT 
ACCACTGCTC AGAAATGGGA TGACTTCTTC CCAGCCCTGA AAGCCAATCA ATCCCTGATA 
AGTCTGGATC TCACAGACAA TAATCTCTTG GACAAGGGTG TGAGGCTTCT ATGTAATACT 
TGGAAGCAAT CAAAGTGCAT ACTGCAGAGG GTATCGTTGG AGAACTGTCA TCTCACTGAA 
GTCTGTTGCA AGGACCTCTC TTCTGTTCTG ATGGTCAGCC AGACTCTAAC ACACCTTAGC 
CTGGCCAAGA ATGAACTTGG AGATAATGGA GTGAAAAAAC TATGTGAAAG CTTAAGCTTC 
CCCAAATGCA AACTACAGAC CCTGGTTCTG TGGTCCTGTA ACATCACCAG CAATGGTTGC 
CACTATCTCT CAAAGATGCT CGGACAAGCA CCAAGCCTGA AGCACTTGGA TCTGGGGCTA 
AATCGCATTG GAACCACAGG TGCAAAGTTT TTGTGTGAGG CTTTGAAGAG CCCCAGGAGT 
AACTTGAAAA GTTTGTGGCT GTGTGGATGC TCCATCATTC CAACGAACTG CAAAGACTTC 
TCTGAGATTC TCAGAAGCAA CAAGAGCCTG AACACTCTAG ACCTGTCTCA GAACGTCATG 
GGAACTGATG CAGTAAAGAT ATTCTGTGAA TCCTTGAAAC TCCAAATTTG TCCCCTCCAA 
ACACTCAGGT TGAAATTTGA TGAAGCTAAC CCCAGTATCC AGAAGCTGAT CCAAGAAATG 
AAGGAGAGCC ATCCACAATT GAGCATTACC AGTGACCAAG TGGATCCTAA GAAAAAGAGT 
CATTCTTTCC CTCATTTCAT TTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001070923.1
CDS	34..3180
Translation	MEHSDQLGFNLKDVLKKLDKTELNNFKSTLKSCSLPQTMKQISKLTMDLANGAQLAEILTDYCPNDWVKRVTFKILEEINRADLAELVMKQVEEAASKASEEKVSSKPREPLKTSLFPQDFVQGDERPEHKHKKEWRNMYTEKWKKNFWPKCNKEIYVDTESYRTLLTLCNPKIEMPFAYTIVLHGPPGSGKTTMARRLMLEWSESKQAQSFSCAFYISCRKLNNSKPCTFAHLLSMNNPSWRYYMIRRLVLSEQFLFVVDGFDELTFPAGALLRDICGDWNTVKPVEVLLSSLLMRKMEPHSTLLVTTQTQSLHQIYLMVDQPVLVETQGFLEPEKQEYFQQYFEDEEGEEEGEGEGKALRALKEVKCNEALFQMASLPAACGIFCLCLELGMKKGEDLALTCQTYTSMFLNFLCRVFLQEACDGHLNKQLQILFKKICILAADSLLEQVPILCEEDFLKLKLNLNKLHPVVYRCIFLEDSSCTNCLSFICLSIQQLLAAIIFVQELGQESKDVSKYSIQKMLSREARLKNPNLSGVLPFVFSLLNKTHIQELETIFGCQISMGVKRKFLECELGENKPFLLLMDLQEIFSCLYESQEEEFVKEAMALFGEVSLHLKTNMDLVHASFCLKNSQNLQTMFLKVEKAVFPENFATLESTAEDQRFQDKKCMLTLWTDFCDTFHSNEKLAFLEIHESFLNSSSLEILCEKLSSAPCHLQKVVLKNISPDDAYEKLCLIFCGYETISHLTLQGGKLDSMLHSLCKVVKNPACNLKFLSLGSCSTTAQKWDDFFPALKANQSLISLDLTDNNLLDKGVRLLCNTWKQSKCILQRVSLENCHLTEVCCKDLSSVLMVSQTLTHLSLAKNELGDNGVKKLCESLSFPKCKLQTLVLWSCNITSNGCHYLSKMLGQAPSLKHLDLGLNRIGTTGAKFLCEALKSPRSNLKSLWLCGCSIIPTNCKDFSEILRSNKSLNTLDLSQNVMGTDAVKIFCESLKLQICPLQTLRLKFDEANPSIQKLIQEMKESHPQLSITSDQVDPKKKSHSFPHFIF

Target ORF information:

RefSeq Version	XM_001070923.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus similar to NACHT, leucine rich repeat and PYD containing 2 (LOC684545), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001070923.3

ORF Insert Sequence:

ATGGAACATT CTGATCAACT GGGTTTCAAC CTAAAAGATG TCCTGAAAAA GCTAGACAAG 
ACCGAGTTGA ACAATTTCAA GAGTACATTG AAGAGCTGTT CTCTACCACA GACAATGAAG 
CAGATCTCCA AGCTGACAAT GGACCTGGCC AATGGAGCTC AACTAGCAGA AATTCTCACA 
GACTACTGCC CCAATGATTG GGTAAAGAGA GTTACCTTCA AGATCTTAGA GGAAATAAAC 
CGTGCAGATC TAGCTGAGTT AGTAATGAAG CAAGTCGAAG AAGCAGCATC AAAAGCATCG 
GAAGAAAAGG TTTCTTCTAA ACCAAGAGAA CCCTTGAAGA CATCACTTTT CCCACAGGAT 
TTCGTTCAAG GAGATGAAAG ACCAGAGCAC AAACATAAAA AGGAATGGAG GAATATGTAC 
ACTGAAAAAT GGAAGAAGAA CTTCTGGCCC AAATGCAATA AAGAAATCTA TGTGGATACT 
GAGAGCTACA GGACACTCCT GACATTGTGT AACCCGAAGA TAGAAATGCC ATTTGCTTAT 
ACAATTGTGC TGCATGGCCC TCCAGGCTCT GGAAAAACCA CAATGGCAAG GAGGCTAATG 
CTAGAATGGT CAGAGAGTAA GCAGGCCCAG AGTTTTTCAT GTGCCTTCTA CATCAGCTGC 
AGGAAACTTA ATAATAGTAA ACCTTGCACT TTTGCCCACC TCCTCTCCAT GAACAATCCC 
TCCTGGAGAT ACTATATGAT TCGGCGTCTA GTCCTGTCAG AACAATTCTT ATTTGTGGTA 
GATGGTTTTG ATGAGCTGAC ATTCCCAGCA GGAGCTCTGC TCCGTGATAT CTGTGGTGAC 
TGGAACACAG TGAAGCCTGT AGAGGTCTTA CTGAGCAGTT TGCTAATGAG GAAGATGGAA 
CCTCATAGCA CACTGCTGGT AACCACACAA ACACAGTCTT TGCACCAAAT CTACCTCATG 
GTAGATCAGC CTGTCCTAGT AGAAACTCAG GGTTTCTTAG AGCCAGAAAA ACAGGAGTAC 
TTTCAGCAAT ACTTTGAAGA TGAGGAGGGT GAGGAAGAGG GTGAAGGTGA GGGAAAGGCA 
CTGAGAGCTC TTAAGGAAGT TAAATGCAAT GAAGCTCTCT TCCAAATGGC CTCACTTCCT 
GCTGCATGTG GAATATTCTG CCTCTGTCTG GAGCTAGGAA TGAAGAAGGG GGAAGACCTG 
GCTCTGACAT GTCAGACCTA CACCTCTATG TTCCTGAACT TCCTCTGCAG AGTCTTCCTA 
CAAGAAGCCT GTGATGGCCA TCTCAACAAG CAACTCCAAA TCTTATTCAA GAAAATATGT 
ATCCTGGCTG CCGATAGTCT CTTAGAACAG GTGCCCATAC TCTGTGAAGA AGACTTCTTG 
AAATTAAAAT TGAACCTGAA TAAATTGCAT CCCGTGGTGT ACAGGTGCAT CTTTCTTGAG 
GACAGTTCCT GTACAAACTG TCTTTCTTTC ATCTGCCTCA GTATCCAGCA GCTCCTGGCT 
GCCATAATAT TTGTTCAGGA ACTCGGGCAA GAAAGCAAGG ATGTCTCTAA ATATAGTATA 
CAGAAGATGC TTTCTAGGGA AGCAAGGTTG AAAAACCCCA ACCTGTCTGG AGTGCTGCCA 
TTTGTATTTA GCCTCCTGAA CAAAACTCAT ATTCAAGAGC TGGAGACCAT TTTTGGCTGC 
CAAATATCAA TGGGGGTCAA GAGAAAATTC CTGGAGTGTG AGTTGGGGGA AAATAAACCC 
TTCCTATTAC TGATGGATCT GCAAGAGATC TTTTCCTGTC TCTATGAATC TCAGGAGGAG 
GAGTTTGTGA AAGAAGCCAT GGCCCTCTTT GGGGAAGTCT CCTTGCATTT GAAAACCAAC 
ATGGACCTCG TCCATGCTTC ATTCTGCCTC AAGAACTCTC AGAACTTGCA GACAATGTTC 
CTTAAAGTAG AAAAGGCAGT CTTCCCAGAG AATTTTGCTA CACTGGAGTC CACAGCTGAG 
GACCAAAGGT TCCAGGACAA AAAATGCATG CTAACTCTCT GGACAGACTT TTGTGACACA 
TTTCATTCAA ATGAGAAACT GGCCTTTCTA GAAATCCACG AAAGCTTCCT CAACAGCTCC 
TCACTGGAGA TTCTTTGTGA AAAGCTGTCC TCTGCTCCCT GCCATCTCCA GAAAGTGGTC 
CTCAAAAATA TTTCCCCAGA TGATGCGTAT GAGAAATTAT GCCTCATTTT TTGTGGTTAT 
GAAACCATCT CACATCTGAC TTTGCAAGGT GGTAAACTGG ATAGCATGCT TCACTCACTG 
TGTAAGGTTG TGAAAAATCC AGCATGCAAC CTGAAGTTTC TCAGCCTGGG ATCTTGTTCT 
ACCACTGCTC AGAAATGGGA TGACTTCTTC CCAGCCCTGA AAGCCAATCA ATCCCTGATA 
AGTCTGGATC TCACAGACAA TAATCTCTTG GACAAGGGTG TGAGGCTTCT ATGTAATACT 
TGGAAGCAAT CAAAGTGCAT ACTGCAGAGG GTATCGTTGG AGAACTGTCA TCTCACTGAA 
GTCTGTTGCA AGGACCTCTC TTCTGTTCTG ATGGTCAGCC AGACTCTAAC ACACCTTAGC 
CTGGCCAAGA ATGAACTTGG AGATAATGGA GTGAAAAAAC TATGTGAAAG CTTAAGCTTC 
CCCAAATGCA AACTACAGAC CCTGGTTCTG TGGTCCTGTA ACATCACCAG CAATGGTTGC 
CACTATCTCT CAAAGATGCT CGGACAAGCA CCAAGCCTGA AGCACTTGGA TCTGGGGCTA 
AATCGCATTG GAACCACAGG TGCAAAGTTT TTGTGTGAGG CTTTGAAGAG CCCCAGGAGT 
AACTTGAAAA GTTTGTGGCT GTGTGGATGC TCCATCATTC CAACGAACTG CAAAGACTTC 
TCTGAGATTC TCAGAAGCAA CAAGAGCCTG AACACTCTAG ACCTGTCTCA GAACGTCATG 
GGAACTGATG CAGTAAAGAT ATTCTGTGAA TCCTTGAAAC TCCAAATTTG TCCCCTCCAA 
ACACTCAGGT TGAAATTTGA TGAAGCTAAC CCCAGTATCC AGAAGCTGAT CCAAGAAATG 
AAGGAGAGCC ATCCACAATT GAGCATTACC AGTGACCAAG TGGATCCTAA GAAAAAGAGT 
CATTCTTTCC CTCATTTCAT TTTTTGA