Rida cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rida
Entrez Gene ID	65151
Full Name	reactive intermediate imine deaminase A homolog
Synonyms	Hrsp12,L-PSP,PSP1,Psp,rp14.5
General protein information	Preferred Names reactive intermediate imine deaminase A homolog Names 2-iminobutanoate/2-iminopropanoate deaminase 14.5 kDa translational inhibitor protein UK114 antigen homolog heat-responsive protein 12 liver perchloric acid-soluble protein perchloric acid-soluble protein perchrolic acid soluble protein ribonuclease UK114 translation inhibitor L-PSP ribonuclease
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q22
Summary	involved in response to heat shock [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031714.1	NP_113902.1	2-iminobutanoate/2-iminopropanoate deaminase

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	hrsp12	NP_001012315.1
Saccharomyces cerevisiae (baker's yeast)	HMF1	NP_010978.3
Gallus gallus (chicken)	HRSP12	XP_001234689.3
Canis lupus familiaris (dog)	HRSP12	XP_532278.3
Mus musculus (house mouse)	Hrsp12	NP_032313.2
Arabidopsis thaliana (thale cress)	AT3G20390	NP_188674.1
	HRSP12	XP_001090682.1
Bos taurus (cattle)	HRSP12	NP_001029380.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005327	XP_315342.3
Caenorhabditis elegans (roundworm)	CELE_C23G10.2	NP_741177.2
Xenopus tropicalis (tropical clawed frog)	hrp12	XP_004915188.1
Homo sapiens (human)	HRSP12	NP_005827.1
Pan troglodytes (chimpanzee)	HRSP12	XP_001149111.1
Rattus norvegicus (Norway rat)	Hrsp12	NP_113902.1
Drosophila melanogaster (fruit fly)	UK114	NP_001260465.1
Oryza sativa (rice)	Os07g0516200	NP_001059781.1

Gene Ontology
Bibliography

Related articles in PubMed

Protective effect of Phellinus linteus polysaccharide extracts against thioacetamide-induced liver fibrosis in rats: a proteomics analysis.
Wang H, Wu G, Park HJ, Jiang PP, Sit WH, van Griensven LJ, Wan JM
Chinese medicine7(1)23(2012 Oct)

Y-box binding protein 1 and RNase UK114 mediate monocyte chemoattractant protein 1 mRNA stability in vascular smooth muscle cells.
Dhawan L, Liu B, Pytlak A, Kulshrestha S, Blaxall BC, Taubman MB
Molecular and cellular biology32(18)3768-75(2012 Sep)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

Conserved YjgF protein family deaminates reactive enamine/imine intermediates of pyridoxal 5'-phosphate (PLP)-dependent enzyme reactions.
Lambrecht JA, Flynn JM, Downs DM
The Journal of biological chemistry287(5)3454-61(2012 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These studies define new roles for glucocorticoid receptor, Y-box binding protein 1, and RNase UK114 in the formation of a molecular complex that degrades MCP-1 mRNA.
Title: Y-box binding protein 1 and RNase UK114 mediate monocyte chemoattractant protein 1 mRNA stability in vascular smooth muscle cells.

Intestinal PSP expression was induced by approximately 3-fold by oral administration of dietary fat
Title: Perchloric acid-soluble protein is expressed in enterocytes and goblet cells in the intestine and upregulated by dietary lipid.

Expression of PSP protein and mRNA decreased and then gradually increased during liver regeneration
Title: Reduced expression of perchloric acid-soluble protein after partial hepatectomy in rats.

PSP is involved in the cellular response to ER stressors, and that the change in localization of PSP from the endoplasmic reticulum to the nucleus may be associated with endoplasmic reticulum stress responses.
Title: Nuclear transfer of perchloric acid-soluble protein by endoplasmic reticulum stressors.

expression of perchloric acid-soluble protein PSP in rat hepatoma was down regulated as compared with intact tissue.
Title: Down regulation of a novel protein, PSP, in rat hepatoma cdRLh 84-beared tumor.

The following Rida gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rida cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08557	NM_031714.1 Latest version!	Rattus norvegicus reactive intermediate imine deaminase A homolog (Rida), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rida ( NM_031714.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113902.1 Rattus norvegicus reactive intermediate imine deaminase A homolog (Rida), mRNA.

Price & Availability↑

CloneID	ORa08557
Clone ID Related Accession (Same CDS sequence)	NM_031714.1
Accession Version	NM_031714.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	2-iminobutanoate/2-iminopropanoate deaminase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF015949.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF015949.1, FQ209529.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906351, SAMN01906353 [ECO:0000348] ##Evidence-Data-END##

ATGTCGTCAA TAATCAGAAA AGTGATCAGC ACTTCAAAAG CCCCGGCGGC CATTGGTGCC 
TACAGCCAAG CTGTGCTAGT GGACAGGACC ATTTACGTTT CTGGACAGAT AGGCATGGAT 
CCTTCCAGTG GACAACTTGT GCCAGGAGGG GTAGCAGAAG AAGCTAATCA AGCTCTTAAA 
AACCTGGGTG AGATTCTGAA GGCTGCAGGC TGTGACTTCA CTAATGTGGT GAAGACAACT 
GTTTTACTGG CTGACATAAA TGACTTTGGC ACTGTCAATG AGATCTACAA AACATATTTC 
CAGGGTAACC TTCCTGCCAG GGCTGCTTAC CAAGTCGCCG CTTTACCCAA AGGAAGTCGA 
ATTGAAATCG AAGCCATCGC TGTCCAGGGG CCTTTCACCA CAGCAGGACT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113902.1
CDS	58..471
Translation	MSSIIRKVISTSKAPAAIGAYSQAVLVDRTIYVSGQIGMDPSSGQLVPGGVAEEANQALKNLGEILKAAGCDFTNVVKTTVLLADINDFGTVNEIYKTYFQGNLPARAAYQVAALPKGSRIEIEAIAVQGPFTTAGL

Target ORF information:

RefSeq Version	NM_031714.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus reactive intermediate imine deaminase A homolog (Rida), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031714.1

ORF Insert Sequence:

ATGTCGTCAA TAATCAGAAA AGTGATCAGC ACTTCAAAAG CCCCGGCGGC CATTGGTGCC 
TACAGCCAAG CTGTGCTAGT GGACAGGACC ATTTACGTTT CTGGACAGAT AGGCATGGAT 
CCTTCCAGTG GACAACTTGT GCCAGGAGGG GTAGCAGAAG AAGCTAATCA AGCTCTTAAA 
AACCTGGGTG AGATTCTGAA GGCTGCAGGC TGTGACTTCA CTAATGTGGT GAAGACAACT 
GTTTTACTGG CTGACATAAA TGACTTTGGC ACTGTCAATG AGATCTACAA AACATATTTC 
CAGGGTAACC TTCCTGCCAG GGCTGCTTAC CAAGTCGCCG CTTTACCCAA AGGAAGTCGA 
ATTGAAATCG AAGCCATCGC TGTCCAGGGG CCTTTCACCA CAGCAGGACT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Protective effect of Phellinus linteus polysaccharide extracts against thioacetamide-induced liver fibrosis in rats: a proteomics analysis.
Chinese medicine7(1)23(2012 Oct)
Wang H,Wu G,Park HJ,Jiang PP,Sit WH,van Griensven LJ,Wan JM

Y-box binding protein 1 and RNase UK114 mediate monocyte chemoattractant protein 1 mRNA stability in vascular smooth muscle cells.
Molecular and cellular biology32(18)3768-75(2012 Sep)
Dhawan L,Liu B,Pytlak A,Kulshrestha S,Blaxall BC,Taubman MB

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

Conserved YjgF protein family deaminates reactive enamine/imine intermediates of pyridoxal 5'-phosphate (PLP)-dependent enzyme reactions.
The Journal of biological chemistry287(5)3454-61(2012 Jan)
Lambrecht JA,Flynn JM,Downs DM