Pln cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pln
Entrez Gene ID	64672
Full Name	phospholamban
Synonyms	Plm
General protein information	Preferred Names phospholamban Names cardiac phospholamban PLB
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20q11
Summary	may inhibit sarcoplasmic reticulum Ca-ATPase; may play a role in calcium regulation and transport in the heart [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022707.2	NP_073198.1	cardiac phospholamban

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Pln	NP_073198.1
	PLN	XP_002803964.1
Canis lupus familiaris (dog)	PLN	NP_001003332.1
Bos taurus (cattle)	PLN	NP_001096789.1
Mus musculus (house mouse)	Pln	NP_001135399.1

Gene Ontology
Bibliography

Related articles in PubMed

Loss of Protein Kinase Novel 1 (PKN1) is associated with mild systolic and diastolic contractile dysfunction, increased phospholamban Thr17 phosphorylation, and exacerbated ischaemia-reperfusion injury.
Francois AA, Obasanjo-Blackshire K, Clark JE, Boguslavskyi A, Holt MR, Parker PJ, Marber MS, Heads RJ
Cardiovascular research114(1)138-157(2018 Jan)

Widespread control of calcium signaling by a family of SERCA-inhibiting micropeptides.
Anderson DM, Makarewich CA, Anderson KM, Shelton JM, Bezprozvannaya S, Bassel-Duby R, Olson EN
Science signaling9(457)ra119(2016 Dec)

TSH inhibits SERCA2a and the PKA/PLN pathway in rat cardiomyocytes.
Dong J, Gao C, Liu J, Cao Y, Tian L
Oncotarget7(26)39207-39215(2016 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results confirm that AKAP7gamma/delta binding to PLB is important for phosphorylation of PLB.
Title: Phosphorylation state-dependent interaction between AKAP7?/? and phospholamban increases phospholamban phosphorylation.

In reperfused control hearts, inhibition of phospholamban phosphorylation or SERCA (thapsigargin) simulated the cardioprotective effects of ischaemic post-conditioning
Title: Delayed phospholamban phosphorylation in post-conditioned heart favours Ca2+ normalization and contributes to protection.

Diminished phosphorylation of phospholamban by anthrax lethal toxin resulted in acute diastolic heart failure.
Title: Anthrax lethal toxin induces acute diastolic dysfunction in rats through disruption of the phospholamban signaling network.

The amount of sarcoplasmic reticulum Ca2+-ATPase (SERCA) 2a and phospholamban (PLB) protein was decreased and that may play a role in the diastolic dysfunction in SHRSP fatty rats.
Title: Abnormal amounts of intracellular calcium regulatory proteins in SHRSP.Z-Lepr(fa)/IzmDmcr rats with metabolic syndrome and cardiac dysfunction.

Cardiac function and phosphorylation of PLB and cTnI were compared in pacing, isoproterenol treatment, and combined pacing and isoproterenol treatment in isolated working heart.
Title: Synergistic effects between phosphorylation of phospholamban and troponin I promote relaxation at higher heart rate.

The following Pln gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pln cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06309	NM_022707.2 Latest version!	Rattus norvegicus phospholamban (Pln), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pln ( NM_022707.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_073198.1 Rattus norvegicus phospholamban (Pln), mRNA.

Price & Availability↑

CloneID	ORa06309
Clone ID Related Accession (Same CDS sequence)	NM_022707.2
Accession Version	NM_022707.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Rattus norvegicus(Norway rat)
Product	cardiac phospholamban
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FM050525.1, CK356371.1, FM056997.1, FM111426.1 and FM055105.1. On Aug 1, 2012 this sequence version replaced NM_022707.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CK357824.1, CK477878.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121

ATGGAAAAAG TCCAATACCT TACTCGCTCG GCTATCAGGA GAGCCTCGAC TATTGAAATG 
CCCCAGCAAG CGCGTCAGAA CCTCCAGAAC CTCTTTATCA ATTTCTGTCT CATCTTGATA 
TGTCTGCTGC TGATCTGCAT CATTGTGATG CTTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_073198.1
CDS	181..339
Translation	MEKVQYLTRSAIRRASTIEMPQQARQNLQNLFINFCLILICLLLICIIVMLL

Target ORF information:

RefSeq Version	NM_022707.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholamban (Pln), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022707.2

ORF Insert Sequence:

1
61
121

ATGGAAAAAG TCCAATACCT TACTCGCTCG GCTATCAGGA GAGCCTCGAC TATTGAAATG 
CCCCAGCAAG CGCGTCAGAA CCTCCAGAAC CTCTTTATCA ATTTCTGTCT CATCTTGATA 
TGTCTGCTGC TGATCTGCAT CATTGTGATG CTTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of Protein Kinase Novel 1 (PKN1) is associated with mild systolic and diastolic contractile dysfunction, increased phospholamban Thr17 phosphorylation, and exacerbated ischaemia-reperfusion injury.
Cardiovascular research114(1)138-157(2018 Jan)
Francois AA,Obasanjo-Blackshire K,Clark JE,Boguslavskyi A,Holt MR,Parker PJ,Marber MS,Heads RJ

Widespread control of calcium signaling by a family of SERCA-inhibiting micropeptides.
Science signaling9(457)ra119(2016 Dec)
Anderson DM,Makarewich CA,Anderson KM,Shelton JM,Bezprozvannaya S,Bassel-Duby R,Olson EN

TSH inhibits SERCA2a and the PKA/PLN pathway in rat cardiomyocytes.
Oncotarget7(26)39207-39215(2016 Jun)
Dong J,Gao C,Liu J,Cao Y,Tian L