Nphs1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nphs1
Entrez Gene ID	64563
Full Name	NPHS1 nephrin
General protein information	Preferred Names NPHS1 nephrin Names nephrin nephrosis 1 homolog, nephrin nephrosis 1, congenital, Finnish type (nephrin) renal glomerulus-specific cell adhesion receptor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q21
Summary	renal protein localized specifically to glomerular capillary walls; may contribute to the development of proteinuria in renal disease [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008759187.2	XP_008757409.1	nephrin isoform X2
NM_022628.1	NP_072150.1	nephrin precursor
XM_008759186.2	XP_008757408.1	nephrin isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NPHS1	XP_524228.3
Homo sapiens (human)	NPHS1	NP_004637.1
Rattus norvegicus (Norway rat)	Nphs1	NP_072150.1
Mus musculus (house mouse)	Nphs1	NP_062332.2
Drosophila melanogaster (fruit fly)	sns	NP_788286.1
	NPHS1	XP_001112314.1
Canis lupus familiaris (dog)	NPHS1	XP_541685.3
Bos taurus (cattle)	NPHS1	NP_001179441.1
Danio rerio (zebrafish)	nphs1	NP_001035777.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010742	XP_311455.4
Xenopus tropicalis (tropical clawed frog)	nphs1	XP_002940674.2

Gene Ontology
Bibliography

Related articles in PubMed

The WD40-domain containing protein CORO2B is specifically enriched in glomerular podocytes and regulates the ventral actin cytoskeleton.
Rogg M, Yasuda-Yamahara M, Abed A, Dinse P, Helmst?dter M, Conzelmann AC, Frimmel J, Sellung D, Biniossek ML, Kretz O, Grahammer F, Schilling O, Huber TB, Schell C
Scientific reports7(1)15910(2017 Nov)

Metformin ameliorates podocyte damage by restoring renal tissue nephrin expression in type 2 diabetic rats.
Zhai L, Gu J, Yang D, Hu W, Wang W, Ye S
Journal of diabetes9(5)510-517(2017 May)

The effects of sildenafil citrate on urinary podocin and nephrin mRNA expression in an L-NAME model of pre-eclampsia.
Baijnath S, Murugesan S, Mackraj I, Gathiram P, Moodley J
Molecular and cellular biochemistry427(1-2)59-67(2017 Mar)

Candesartan Mediated Amelioration of Cisplatin-Induced Testicular Damage Is Associated with Alterations in Expression Patterns of Nephrin and Podocin.
Enatsu N, Miyake H, Chiba K, Fujisawa M
BioMed research international2015273784(2015)

Increased SHP-1 protein expression by high glucose levels reduces nephrin phosphorylation in podocytes.
Denhez B, Lizotte F, Guimond MO, Jones N, Takano T, Geraldes P
The Journal of biological chemistry290(1)350-8(2015 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that metformin provides some protection against podocyte damage in diabetic rats, which may be related, in part, to its effect in reducing urinary nephrin (Nphs1) excretion as well as improving podocyte nephrin expression.
Title: Metformin ameliorates podocyte damage by restoring renal tissue nephrin expression in type 2 diabetic rats.

The effects of sildenafil citrate on urinary podocin and nephrin mRNA expression in an L-NAME model of pre-eclampsia.
Title: The effects of sildenafil citrate on urinary podocin and nephrin mRNA expression in an L-NAME model of pre-eclampsia.

These findings suggest that candesartan can prevent cisplatin-induced testicular damage by regulating expression patterns of the nephrin-podocin complex in the testes.
Title: Candesartan Mediated Amelioration of Cisplatin-Induced Testicular Damage Is Associated with Alterations in Expression Patterns of Nephrin and Podocin.

Rat nephrin Tyr1127 and Tyr1152 are essential for SHP-1 induced nephrin dephosphorylation under high-glucose conditions.
Title: Increased SHP-1 protein expression by high glucose levels reduces nephrin phosphorylation in podocytes.

Irbesartan treatment ameliorates proteinuria in puromycin aminonucleoside nephropathy by inhibiting the reduction of NEPH1 and nephrin in the kidney glomerulus.
Title: Angiotensin II type 1 receptor blockade ameliorates proteinuria in puromycin aminonucleoside nephropathy by inhibiting the reduction of NEPH1 and nephrin.

The following Nphs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nphs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa06172	NM_022628.1 Latest version!	Rattus norvegicus NPHS1 adhesion molecule, nephrin (Nphs1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa43609	XM_008759186.2 Latest version!	Rattus norvegicus NPHS1 nephrin (Nphs1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa43610	XM_008759187.2 Latest version!	Rattus norvegicus NPHS1 nephrin (Nphs1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nphs1 ( NM_022628.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_072150.1 Rattus norvegicus NPHS1 adhesion molecule, nephrin (Nphs1), mRNA.

Price & Availability↑

CloneID	ORa06172
Clone ID Related Accession (Same CDS sequence)	NM_022628.1
Accession Version	NM_022628.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	nephrin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF161715.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF161715.1, AF172255.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02642745, SAMN02642747 [ECO:0000348] ##Evidence-Data-END##

ATGGGCGCTA AGAGAGTCAC TGTCAGAGGT GCCCGGACAA GCCCAATACA CAGAACGTCC 
AGTTTGACTC CCCTGCTGCT CATGGGAATG CTGACCTCAG GCCTGGCCGA GTCGCCAGTC 
CCCACCTCAG CACCTCAAGG CTTCTGGGCT CTGTCTGAAA ACCTGACTGC GGTGGAAGGG 
ACAACAGTTA AGCTATGGTG CGGTGTCAGG GCCCCTGGCA GTGTGGTGCA GTGGGCTAAG 
GATGGGCTGC TTCTGGGTCC AAACCCGAAG ATGCCAGGCT TCCCGAGGTA CAGCCTGGAA 
GGAGATCGTG CTAAAGGCGA GTTCCACCTG CTTATTGAAG CCTGTGACCT CAGTGATGAC 
GCAGAGTATG AATGCCAAGT CGGCCGCTCA GAGTTGGGTC CCGAGCTTGT GTCTCCTAAA 
GTAATCCTCT CCATTCTAGT TTCCCCCAAG GTGCTTCTGT TGACCCCCGA GGCAGGAAGC 
ACAGTGACCT GGGTAGCTGG GCAGGAGTAT GTGGTCACCT GTGTGTCTGG AGATGCAAAA 
CCAGCACCTG ACATCACCTT CATCCAGAGT GGACGAACTA TATTGGACGT CTCCTCCAAT 
GTGAATGAGG GATCAGAGGA GAAACTCTGC ATCACAGAGG CCGAAGCCAG GGTGATACCC 
CAGAGCTCGG ATAACGGGCA GTTACTGGTC TGTGAGGGTT CCAACCCAGC TTTGGACACT 
CCCATAAAGG CTTCATTCAC CATGAATATT CTGTTTCCCC CAGGACCTCC TGTCATTGAT 
TGGCCAGGCC TGAATGAGGG GCATGTGAGG GCAGGGGAGA ACCTGGAGCT GCCCTGCACA 
GCCAGAGGTG GCAATCCACC TGCTACCCTG CAGTGGCTGA AGAACGGTAA ACCAGTGTCC 
ACAGCCTGGG GCACCGAGCA TGCCCAGGCA GTGGCCCACA GCGTGCTGGT GATGACTGTA 
CGACCTGAAG ACCATGGAGC TCGGCTCAGC TGTCAGTCCT ACAACAGCGT GTCTGCAGGG 
ACCCAGGAGA GAAGCATCAC ACTACAGGTC ACCTTTCCCC CAAGCGCCAT TACCATCCTG 
GGATCTGTAT CACAATCGGA GAACAAGAAC GTGACCCTTT GCTGCCTGAC CAAGTCCAGT 
CGCCCACGGG TCCTGCTGCG ATGGTGGTTG GGTGGACGGC AGCTGCTGCC CACAGATGAG 
ACAGTCATGG ATGGCCTGCA TGGTGGCCAC ATCTCCATGT CCAATCTCAC ATTCTTGGTG 
CGGAGAGAAG ACAATGGCCT GCCCCTCACC TGTGAAGCCT TCAGTGACGC CTTCAGCAAG 
GAGACCTTCA AGAAGTCACT CACCTTGAAT GTGAAATACC CTGCCCAGAA GCTGTGGATT 
GAGGGGCCCC CAGAGGGACA GTACATCCGG ACTGGGACTC GGGTGAGGCT GGTATGCTTG 
GCCATCGGAG GCAACCCAGA CCCCTCCCTC ATCTGGTTTA AGGATTCACG TCCGGTGAGC 
GAGCCCCGGC AGCCCCAGGA GCCCCGGCGT GTGCAGCTGG GCAGTGTGGA GAAGTCCGGG 
AGCACTTTCT CCCGCGAGCT GGTGTTGATC ATAGGTCCGC CGGACAACCG AGCCAAGTTC 
TCCTGCAAGG CGGGTCAGCT CAGTGCGTCT ACGCAGCTGG TGGTGCAGTT CCCCCCAACC 
AACCTGACCA TCCTGGCCAA CTCGTCCGCG CTGCGCCCAG GCGACGCCTT GAACTTGACC 
TGCGTCAGCA TCAGCAGCAA CCCCCCAGTC AACTTGTCTT GGGACAAGGA AGGAGAGAGG 
CTGGAAGATG TGGCTGCAAA ACCCCAGAGT GCACCGTTCA AAGGCTCCGC TGCATCCAGG 
AGTGTTTTTC TCAGAGTGTC ATCCCGAGAC CACGGTCAAC GGGTCACCTG CCGGGCCCAC 
AGCGAGGCAC TCCGTGAAAC CGTGAGCTCC TTCTACCGCT TCAATGTGCT GTATCCTCCA 
GAATTCCTGG GGGAGCAAGT CCGGGCAGTG ACCGTGGTGG AGCAGGGCCA GGTGCTGCTG 
CCGGTGTCGG TGTCCGCTAA CCCCGCCCCC GAGGCCTTCA ACTGGACCTT CCGAGGCTAC 
CGCCTCAGCC CAGCTGGGGG TCCCCGGCAC CGTATCCTGT CTGGAGGGGC TCTGCAGCTG 
TGGAATGTGA CCCGAGCTGA CGATGGCTTT TATCAGCTGC ACTGCCAGAA CTCAGAGGGC 
ACCGCTGAGG CGCTGTTGAA GCTGGACGTG CATTATGCTC CCACCATCCG TGCCCTCCGG 
GACCCTACTG AGGTGAATGT TGGGGGTTCT GTGGACATAG TCTGCACCGT TGACGCCAAT 
CCCATCCTCC CAGAGATGTT CAGCTGGGAG AGACTGGGAG AAGAAGAGGA GGATCTGAAC 
CTGGACGACA TGGAGAAAGT TTCCAAGGGA TCCACGGGGC GTCTGCGGAT TCGCCAAGCC 
AAGCTATCCC AGGCTGGTGC CTACCAGTGC ATCGTGGACA ATGGGGTGGC TCCTGCAGCC 
AGAGGACTGG TTCGTCTTGT CGTCCGATTT GCTCCCCAGG TGGATCAGCC TACTCCCCTA 
ACAAAAGTGG CTGCCGCTGG GGACAGCACC AGCTCAGCCA CACTGCACTG CCGTGCCCGG 
GGTGTCCCCA ACATCGACTT CACTTGGACC AAAAACGGGG TCCCTCTGGA TCTCCAAGAC 
CCCAGGTACA CAGAGCACAG GTACCACCAG GGTGTTGTCC ACAGCAGCCT CTTGACCATC 
GCTAATGTGT CTGCGGCCCA GGACTATGCC CTCTTCAAAT GCACGGCCAC CAATGCCCTT 
GGCTCTGACC ACACCAACAT CCAGCTCGTC AGCATCAGCC GCCCTGACCC TCCACTGGGA 
CTGAAGGTTG TCAGCATAAG CCCTCACTCG GTGGGGCTGG AGTGGAAGCC TGGCTTTGAT 
GGGGGTCTGC CTCAGAGGTT CCAAATCAGG TACGAGGCCC TCGAGACCCC AGGATTCCTC 
CACGTGGATG TCCTACCTAC ACAGGCCACT ACCTTCACGC TGACTGGGCT GAAGCCTTCT 
ACACGATATA GGATCTGGCT GTTGGCCAGC AATGCCCTGG GGGACAGTGG ATTGACGGAC 
AAGGGGATCC AGGTCTCCGT CACTACCCCA GGCCCCGACC AGGCTCCTGA AGACACAGAC 
CACCAGCTGC CCACAGAGCT GCCTCCAGGA CCCCCAAGGC TGCCCCTGCT GCCTGTGCTC 
TTTGCAGTTG GTGGTCTTCT GCTGCTCTCC AATGCCTCCT GTGTTGGGGG TCTCCTCTGG 
CGGAGAAGAC TGAGGCGCCT TGCTGAGGAG ATCTCAGAGA AGACAGAGGC AGGGTCGGAG 
GACAGGATCA GGAATGAATA TGAGGAGAGT CAGTGGACTG GGGACCGGGA CACGAGAAGC 
TCCACGGTTA GCACAGCAGA AGTGGACCCA AATTACTACT CCATGAGGGA CTTCAGCCCC 
CAGCTTCCCC CAACACTGGA GGAGGTGCTG TATCACCAAG GTGCTGAAGG CGAGGACATG 
GCCTTCCCCG GACACCTGCA TGATGAAGTG GAGAGAGCCT ATGGCCCGCC TGGGGCCTGG 
GGACCCCTCT ATGATGAAGT ACGAATGGAC CCCTATGACC TTCGCTGGCC TGAGGTCCAG 
TGTGAGGATC CGAGGGGAAT CTACGACCAG GTGGCAGCAG ACATGGATGC TGTGGAAGCT 
AGCTCTCTGC CGTTTGAGCT GAGGGGACAT CTGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_072150.1
CDS	404..4162
Translation	MGAKRVTVRGARTSPIHRTSSLTPLLLMGMLTSGLAESPVPTSAPQGFWALSENLTAVEGTTVKLWCGVRAPGSVVQWAKDGLLLGPNPKMPGFPRYSLEGDRAKGEFHLLIEACDLSDDAEYECQVGRSELGPELVSPKVILSILVSPKVLLLTPEAGSTVTWVAGQEYVVTCVSGDAKPAPDITFIQSGRTILDVSSNVNEGSEEKLCITEAEARVIPQSSDNGQLLVCEGSNPALDTPIKASFTMNILFPPGPPVIDWPGLNEGHVRAGENLELPCTARGGNPPATLQWLKNGKPVSTAWGTEHAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSAGTQERSITLQVTFPPSAITILGSVSQSENKNVTLCCLTKSSRPRVLLRWWLGGRQLLPTDETVMDGLHGGHISMSNLTFLVRREDNGLPLTCEAFSDAFSKETFKKSLTLNVKYPAQKLWIEGPPEGQYIRTGTRVRLVCLAIGGNPDPSLIWFKDSRPVSEPRQPQEPRRVQLGSVEKSGSTFSRELVLIIGPPDNRAKFSCKAGQLSASTQLVVQFPPTNLTILANSSALRPGDALNLTCVSISSNPPVNLSWDKEGERLEDVAAKPQSAPFKGSAASRSVFLRVSSRDHGQRVTCRAHSEALRETVSSFYRFNVLYPPEFLGEQVRAVTVVEQGQVLLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALRDPTEVNVGGSVDIVCTVDANPILPEMFSWERLGEEEEDLNLDDMEKVSKGSTGRLRIRQAKLSQAGAYQCIVDNGVAPAARGLVRLVVRFAPQVDQPTPLTKVAAAGDSTSSATLHCRARGVPNIDFTWTKNGVPLDLQDPRYTEHRYHQGVVHSSLLTIANVSAAQDYALFKCTATNALGSDHTNIQLVSISRPDPPLGLKVVSISPHSVGLEWKPGFDGGLPQRFQIRYEALETPGFLHVDVLPTQATTFTLTGLKPSTRYRIWLLASNALGDSGLTDKGIQVSVTTPGPDQAPEDTDHQLPTELPPGPPRLPLLPVLFAVGGLLLLSNASCVGGLLWRRRLRRLAEEISEKTEAGSEDRIRNEYEESQWTGDRDTRSSTVSTAEVDPNYYSMRDFSPQLPPTLEEVLYHQGAEGEDMAFPGHLHDEVERAYGPPGAWGPLYDEVRMDPYDLRWPEVQCEDPRGIYDQVAADMDAVEASSLPFELRGHLV

Target ORF information:

RefSeq Version	NM_022628.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NPHS1 adhesion molecule, nephrin (Nphs1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022628.1

ORF Insert Sequence:

ATGGGCGCTA AGAGAGTCAC TGTCAGAGGT GCCCGGACAA GCCCAATACA CAGAACGTCC 
AGTTTGACTC CCCTGCTGCT CATGGGAATG CTGACCTCAG GCCTGGCCGA GTCGCCAGTC 
CCCACCTCAG CACCTCAAGG CTTCTGGGCT CTGTCTGAAA ACCTGACTGC GGTGGAAGGG 
ACAACAGTTA AGCTATGGTG CGGTGTCAGG GCCCCTGGCA GTGTGGTGCA GTGGGCTAAG 
GATGGGCTGC TTCTGGGTCC AAACCCGAAG ATGCCAGGCT TCCCGAGGTA CAGCCTGGAA 
GGAGATCGTG CTAAAGGCGA GTTCCACCTG CTTATTGAAG CCTGTGACCT CAGTGATGAC 
GCAGAGTATG AATGCCAAGT CGGCCGCTCA GAGTTGGGTC CCGAGCTTGT GTCTCCTAAA 
GTAATCCTCT CCATTCTAGT TTCCCCCAAG GTGCTTCTGT TGACCCCCGA GGCAGGAAGC 
ACAGTGACCT GGGTAGCTGG GCAGGAGTAT GTGGTCACCT GTGTGTCTGG AGATGCAAAA 
CCAGCACCTG ACATCACCTT CATCCAGAGT GGACGAACTA TATTGGACGT CTCCTCCAAT 
GTGAATGAGG GATCAGAGGA GAAACTCTGC ATCACAGAGG CCGAAGCCAG GGTGATACCC 
CAGAGCTCGG ATAACGGGCA GTTACTGGTC TGTGAGGGTT CCAACCCAGC TTTGGACACT 
CCCATAAAGG CTTCATTCAC CATGAATATT CTGTTTCCCC CAGGACCTCC TGTCATTGAT 
TGGCCAGGCC TGAATGAGGG GCATGTGAGG GCAGGGGAGA ACCTGGAGCT GCCCTGCACA 
GCCAGAGGTG GCAATCCACC TGCTACCCTG CAGTGGCTGA AGAACGGTAA ACCAGTGTCC 
ACAGCCTGGG GCACCGAGCA TGCCCAGGCA GTGGCCCACA GCGTGCTGGT GATGACTGTA 
CGACCTGAAG ACCATGGAGC TCGGCTCAGC TGTCAGTCCT ACAACAGCGT GTCTGCAGGG 
ACCCAGGAGA GAAGCATCAC ACTACAGGTC ACCTTTCCCC CAAGCGCCAT TACCATCCTG 
GGATCTGTAT CACAATCGGA GAACAAGAAC GTGACCCTTT GCTGCCTGAC CAAGTCCAGT 
CGCCCACGGG TCCTGCTGCG ATGGTGGTTG GGTGGACGGC AGCTGCTGCC CACAGATGAG 
ACAGTCATGG ATGGCCTGCA TGGTGGCCAC ATCTCCATGT CCAATCTCAC ATTCTTGGTG 
CGGAGAGAAG ACAATGGCCT GCCCCTCACC TGTGAAGCCT TCAGTGACGC CTTCAGCAAG 
GAGACCTTCA AGAAGTCACT CACCTTGAAT GTGAAATACC CTGCCCAGAA GCTGTGGATT 
GAGGGGCCCC CAGAGGGACA GTACATCCGG ACTGGGACTC GGGTGAGGCT GGTATGCTTG 
GCCATCGGAG GCAACCCAGA CCCCTCCCTC ATCTGGTTTA AGGATTCACG TCCGGTGAGC 
GAGCCCCGGC AGCCCCAGGA GCCCCGGCGT GTGCAGCTGG GCAGTGTGGA GAAGTCCGGG 
AGCACTTTCT CCCGCGAGCT GGTGTTGATC ATAGGTCCGC CGGACAACCG AGCCAAGTTC 
TCCTGCAAGG CGGGTCAGCT CAGTGCGTCT ACGCAGCTGG TGGTGCAGTT CCCCCCAACC 
AACCTGACCA TCCTGGCCAA CTCGTCCGCG CTGCGCCCAG GCGACGCCTT GAACTTGACC 
TGCGTCAGCA TCAGCAGCAA CCCCCCAGTC AACTTGTCTT GGGACAAGGA AGGAGAGAGG 
CTGGAAGATG TGGCTGCAAA ACCCCAGAGT GCACCGTTCA AAGGCTCCGC TGCATCCAGG 
AGTGTTTTTC TCAGAGTGTC ATCCCGAGAC CACGGTCAAC GGGTCACCTG CCGGGCCCAC 
AGCGAGGCAC TCCGTGAAAC CGTGAGCTCC TTCTACCGCT TCAATGTGCT GTATCCTCCA 
GAATTCCTGG GGGAGCAAGT CCGGGCAGTG ACCGTGGTGG AGCAGGGCCA GGTGCTGCTG 
CCGGTGTCGG TGTCCGCTAA CCCCGCCCCC GAGGCCTTCA ACTGGACCTT CCGAGGCTAC 
CGCCTCAGCC CAGCTGGGGG TCCCCGGCAC CGTATCCTGT CTGGAGGGGC TCTGCAGCTG 
TGGAATGTGA CCCGAGCTGA CGATGGCTTT TATCAGCTGC ACTGCCAGAA CTCAGAGGGC 
ACCGCTGAGG CGCTGTTGAA GCTGGACGTG CATTATGCTC CCACCATCCG TGCCCTCCGG 
GACCCTACTG AGGTGAATGT TGGGGGTTCT GTGGACATAG TCTGCACCGT TGACGCCAAT 
CCCATCCTCC CAGAGATGTT CAGCTGGGAG AGACTGGGAG AAGAAGAGGA GGATCTGAAC 
CTGGACGACA TGGAGAAAGT TTCCAAGGGA TCCACGGGGC GTCTGCGGAT TCGCCAAGCC 
AAGCTATCCC AGGCTGGTGC CTACCAGTGC ATCGTGGACA ATGGGGTGGC TCCTGCAGCC 
AGAGGACTGG TTCGTCTTGT CGTCCGATTT GCTCCCCAGG TGGATCAGCC TACTCCCCTA 
ACAAAAGTGG CTGCCGCTGG GGACAGCACC AGCTCAGCCA CACTGCACTG CCGTGCCCGG 
GGTGTCCCCA ACATCGACTT CACTTGGACC AAAAACGGGG TCCCTCTGGA TCTCCAAGAC 
CCCAGGTACA CAGAGCACAG GTACCACCAG GGTGTTGTCC ACAGCAGCCT CTTGACCATC 
GCTAATGTGT CTGCGGCCCA GGACTATGCC CTCTTCAAAT GCACGGCCAC CAATGCCCTT 
GGCTCTGACC ACACCAACAT CCAGCTCGTC AGCATCAGCC GCCCTGACCC TCCACTGGGA 
CTGAAGGTTG TCAGCATAAG CCCTCACTCG GTGGGGCTGG AGTGGAAGCC TGGCTTTGAT 
GGGGGTCTGC CTCAGAGGTT CCAAATCAGG TACGAGGCCC TCGAGACCCC AGGATTCCTC 
CACGTGGATG TCCTACCTAC ACAGGCCACT ACCTTCACGC TGACTGGGCT GAAGCCTTCT 
ACACGATATA GGATCTGGCT GTTGGCCAGC AATGCCCTGG GGGACAGTGG ATTGACGGAC 
AAGGGGATCC AGGTCTCCGT CACTACCCCA GGCCCCGACC AGGCTCCTGA AGACACAGAC 
CACCAGCTGC CCACAGAGCT GCCTCCAGGA CCCCCAAGGC TGCCCCTGCT GCCTGTGCTC 
TTTGCAGTTG GTGGTCTTCT GCTGCTCTCC AATGCCTCCT GTGTTGGGGG TCTCCTCTGG 
CGGAGAAGAC TGAGGCGCCT TGCTGAGGAG ATCTCAGAGA AGACAGAGGC AGGGTCGGAG 
GACAGGATCA GGAATGAATA TGAGGAGAGT CAGTGGACTG GGGACCGGGA CACGAGAAGC 
TCCACGGTTA GCACAGCAGA AGTGGACCCA AATTACTACT CCATGAGGGA CTTCAGCCCC 
CAGCTTCCCC CAACACTGGA GGAGGTGCTG TATCACCAAG GTGCTGAAGG CGAGGACATG 
GCCTTCCCCG GACACCTGCA TGATGAAGTG GAGAGAGCCT ATGGCCCGCC TGGGGCCTGG 
GGACCCCTCT ATGATGAAGT ACGAATGGAC CCCTATGACC TTCGCTGGCC TGAGGTCCAG 
TGTGAGGATC CGAGGGGAAT CTACGACCAG GTGGCAGCAG ACATGGATGC TGTGGAAGCT 
AGCTCTCTGC CGTTTGAGCT GAGGGGACAT CTGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nphs1 ( XM_008759186.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757408.1 Rattus norvegicus NPHS1 nephrin (Nphs1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43609
Clone ID Related Accession (Same CDS sequence)	XM_008759186.2
Accession Version	XM_008759186.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nephrin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759186.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGTC TGACGGCTTT GGCTTTCTGG GTTCATGGAA CTGCTGGACC CAGCACAGCT 
GGCCTGGCCG AGTCGCCAGT CCCCACCTCA GCACCTCAAG GCTTCTGGGC TCTGTCTGAA 
AACCTGACTG CGGTGGAAGG GACAACAGTT AAGCTATGGT GCGGTGTCAG GGCCCCTGGC 
AGTGTGGTGC AGTGGGCTAA GGATGGGCTG CTTCTGGGTC CAAACCCGAA GATGCCAGGC 
TTCCCGAGGT ACAGCCTGGA AGGAGATCGT GCTAAAGGCG AGTTCCACCT GCTTATTGAA 
GCCTGTGACC TCAGTGATGA CGCAGAGTAT GAATGCCAAG TCGGCCGCTC AGAGTTGGGT 
CCCGAGCTTG TGTCTCCTAA AGTAATCCTC TCCATTCTAG TTTCCCCCAA GGTGCTTCTG 
TTGACCCCCG AGGCAGGAAG CACAGTGACC TGGGTAGCTG GGCAGGAGTA TGTGGTCACC 
TGTGTGTCTG GAGATGCAAA ACCAGCACCT GACATCACCT TCATCCAGAG TGGACGAACT 
ATATTGGACG TCTCCTCCAA TGTGAATGAG GGATCAGAGG AGAAACTCTG CATCACAGAG 
GCCGAAGCCA GGGTGATACC CCAGAGCTCG GATAACGGGC AGTTACTGGT CTGTGAGGGT 
TCCAACCCAG CTTTGGACAC TCCCATAAAG GCTTCATTCA CCATGAATAT TCTGTTTCCC 
CCAGGACCTC CTGTCATTGA TTGGCCAGGC CTGAATGAGG GGCATGTGAG GGCAGGGGAG 
AACCTGGAGC TGCCCTGCAC AGCCAGAGGT GGCAATCCAC CTGCTACCCT GCAGTGGCTG 
AAGAACGGTA AACCAGTGTC CACAGCCTGG GGCACCGAGC ATGCCCAGGC AGTGGCCCAC 
AGCGTGCTGG TGATGACTGT ACGACCTGAA GACCATGGAG CTCGGCTCAG CTGTCAGTCC 
TACAACAGCG TGTCTGCAGG GACCCAGGAG AGAAGCATCA CACTACAGGT CACCTTTCCC 
CCAAGCGCCA TTACCATCCT GGGATCTGTA TCACAATCGG AGAACAAGAA CGTGACCCTT 
TGCTGCCTGA CCAAGTCCAG TCGCCCACGG GTCCTGCTGC GATGGTGGTT GGGTGGACGG 
CAGCTGCTGC CCACAGATGA GACAGTCATG GATGGCCTGC ATGGTGGCCA CATCTCCATG 
TCCAATCTCA CATTCTTGGT GCGGAGAGAA GACAATGGCC TGCCCCTCAC CTGTGAAGCC 
TTCAGTGACG CCTTCAGCAA GGAGACCTTC AAGAAGTCAC TCACCTTGAA TGTGAAATAC 
CCTGCCCAGA AGCTGTGGAT TGAGGGGCCC CCAGAGGGAC AGTACATCCG GACTGGGACT 
CGGGTGAGGC TGGTATGCTT GGCCATCGGA GGCAACCCAG ACCCCTCCCT CATCTGGTTT 
AAGGATTCAC GTCCGGTGAG CGAGCCCCGG CAGCCCCAGG AGCCCCGGCG TGTGCAGCTG 
GGCAGTGTGG AGAAGTCCGG GAGCACTTTC TCCCGCGAGC TGGTGTTGAT CATAGGTCCG 
CCGGACAACC GAGCCAAGTT CTCCTGCAAG GCGGGTCAGC TCAGTGCGTC TACGCAGCTG 
GTGGTGCAGT TCCCCCCAAC CAACCTGACC ATCCTGGCCA ACTCGTCCGC GCTGCGCCCA 
GGCGACGCCT TGAACTTGAC CTGCGTCAGC ATCAGCAGCA ACCCCCCAGT CAACTTGTCT 
TGGGACAAGG AAGGAGAGAG GCTGGAAGAT GTGGCTGCAA AACCCCAGAG TGCACCGTTC 
AAAGGCTCCG CTGCATCCAG GAGTGTTTTT CTCAGAGTGT CATCCCGAGA CCACGGTCAA 
CGGGTCACCT GCCGGGCCCA CAGCGAGGCA CTCCGTGAAA CCGTGAGCTC CTTCTACCGC 
TTCAATGTGC TGTATCCTCC AGAATTCCTG GGGGAGCAAG TCCGGGCAGT GACCGTGGTG 
GAGCAGGGCC AGGTGCTGCT GCCGGTGTCG GTGTCCGCTA ACCCCGCCCC CGAGGCCTTC 
AACTGGACCT TCCGAGGCTA CCGCCTCAGC CCAGCTGGGG GTCCCCGGCA CCGTATCCTG 
TCTGGAGGGG CTCTGCAGCT GTGGAATGTG ACCCGAGCTG ACGATGGCTT TTATCAGCTG 
CACTGCCAGA ACTCAGAGGG CACCGCTGAG GCGCTGTTGA AGCTGGACGT GCATTATGCT 
CCCACCATCC GTGCCCTCCG GGACCCTACT GAGGTGAATG TTGGGGGTTC TGTGGACATA 
GTCTGCACCG TTGACGCCAA TCCCATCCTC CCAGAGATGT TCAGCTGGGA GAGACTGGGA 
GAAGAAGAGG AGGATCTGAA CCTGGACGAC ATGGAGAAAG TTTCCAAGGG ATCCACGGGG 
CGTCTGCGGA TTCGCCAAGC CAAGCTATCC CAGGCTGGTG CCTACCAGTG CATCGTGGAC 
AATGGGGTGG CTCCTGCAGC CAGAGGACTG GTTCGTCTTG TCGTCCGATT TGCTCCCCAG 
GTGGATCAGC CTACTCCCCT AACAAAAGTG GCTGCCGCTG GGGACAGCAC CAGCTCAGCC 
ACACTGCACT GCCGTGCCCG GGGTGTCCCC AACATCGACT TCACTTGGAC CAAAAACGGG 
GTCCCTCTGG ATCTCCAAGA CCCCAGGTAC ACAGAGCACA GGTACCACCA GGGTGTTGTC 
CACAGCAGCC TCTTGACCAT CGCTAATGTG TCTGCGGCCC AGGACTATGC CCTCTTCAAA 
TGCACGGCCA CCAATGCCCT TGGCTCTGAC CACACCAACA TCCAGCTCGT CAGCATCAGC 
CGCCCTGACC CTCCACTGGG ACTGAAGGTT GTCAGCATAA GCCCTCACTC GGTGGGGCTG 
GAGTGGAAGC CTGGCTTTGA TGGGGGTCTG CCTCAGAGGT TCCAAATCAG GTACGAGGCC 
CTCGAGACCC CAGGATTCCT CCACGTGGAT GTCCTACCTA CACAGGCCAC TACCTTCACG 
CTGACTGGGC TGAAGCCTTC TACACGATAT AGGATCTGGC TGTTGGCCAG CAATGCCCTG 
GGGGACAGTG GATTGACGGA CAAGGGGATC CAGGTCTCCG TCACTACCCC AGGCCCCGAC 
CAGGCTCCTG AAGACACAGA CCACCAGCTG CCCACAGAGC TGCCTCCAGG ACCCCCAAGG 
CTGCCCCTGC TGCCTGTGCT CTTTGCAGTT GGTGGTCTTC TGCTGCTCTC CAATGCCTCC 
TGTGTTGGGG GTCTCCTCTG GCGGAGAAGA CTGAGGCGCC TTGCTGAGGA GATCTCAGAG 
AAGACAGAGG CAGGGTCGGA GGACAGGATC AGGAATGAAT ATGAGGAGAG TCAGTGGACT 
GGGGACCGGG ACACGAGAAG CTCCACGGTT AGCACAGCAG AAGTGGACCC AAATTACTAC 
TCCATGAGGG ACTTCAGCCC CCAGCTTCCC CCAACACTGG AGGAGGTGCT GTATCACCAA 
GGTGCTGAAG GCGAGGACAT GGCCTTCCCC GGACACCTGC ATGATGAAGT GGAGAGAGCC 
TATGGCCCGC CTGGGGCCTG GGGACCCCTC TATGATGAAG TACGAATGGA CCCCTATGAC 
CTTCGCTGGC CTGAGGTCCA GTGTGAGGAT CCGAGGGGAA TCTACGACCA GGTGGCAGCA 
GACATGGATG CTGTGGAAGC TAGCTCTCTG CCGTTTGAGC TGAGGGGACA TCTGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757408.1
CDS	123..3842
Translation	MLCLTALAFWVHGTAGPSTAGLAESPVPTSAPQGFWALSENLTAVEGTTVKLWCGVRAPGSVVQWAKDGLLLGPNPKMPGFPRYSLEGDRAKGEFHLLIEACDLSDDAEYECQVGRSELGPELVSPKVILSILVSPKVLLLTPEAGSTVTWVAGQEYVVTCVSGDAKPAPDITFIQSGRTILDVSSNVNEGSEEKLCITEAEARVIPQSSDNGQLLVCEGSNPALDTPIKASFTMNILFPPGPPVIDWPGLNEGHVRAGENLELPCTARGGNPPATLQWLKNGKPVSTAWGTEHAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSAGTQERSITLQVTFPPSAITILGSVSQSENKNVTLCCLTKSSRPRVLLRWWLGGRQLLPTDETVMDGLHGGHISMSNLTFLVRREDNGLPLTCEAFSDAFSKETFKKSLTLNVKYPAQKLWIEGPPEGQYIRTGTRVRLVCLAIGGNPDPSLIWFKDSRPVSEPRQPQEPRRVQLGSVEKSGSTFSRELVLIIGPPDNRAKFSCKAGQLSASTQLVVQFPPTNLTILANSSALRPGDALNLTCVSISSNPPVNLSWDKEGERLEDVAAKPQSAPFKGSAASRSVFLRVSSRDHGQRVTCRAHSEALRETVSSFYRFNVLYPPEFLGEQVRAVTVVEQGQVLLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALRDPTEVNVGGSVDIVCTVDANPILPEMFSWERLGEEEEDLNLDDMEKVSKGSTGRLRIRQAKLSQAGAYQCIVDNGVAPAARGLVRLVVRFAPQVDQPTPLTKVAAAGDSTSSATLHCRARGVPNIDFTWTKNGVPLDLQDPRYTEHRYHQGVVHSSLLTIANVSAAQDYALFKCTATNALGSDHTNIQLVSISRPDPPLGLKVVSISPHSVGLEWKPGFDGGLPQRFQIRYEALETPGFLHVDVLPTQATTFTLTGLKPSTRYRIWLLASNALGDSGLTDKGIQVSVTTPGPDQAPEDTDHQLPTELPPGPPRLPLLPVLFAVGGLLLLSNASCVGGLLWRRRLRRLAEEISEKTEAGSEDRIRNEYEESQWTGDRDTRSSTVSTAEVDPNYYSMRDFSPQLPPTLEEVLYHQGAEGEDMAFPGHLHDEVERAYGPPGAWGPLYDEVRMDPYDLRWPEVQCEDPRGIYDQVAADMDAVEASSLPFELRGHLV

Target ORF information:

RefSeq Version	XM_008759186.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NPHS1 nephrin (Nphs1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759186.2

ORF Insert Sequence:

ATGCTGTGTC TGACGGCTTT GGCTTTCTGG GTTCATGGAA CTGCTGGACC CAGCACAGCT 
GGCCTGGCCG AGTCGCCAGT CCCCACCTCA GCACCTCAAG GCTTCTGGGC TCTGTCTGAA 
AACCTGACTG CGGTGGAAGG GACAACAGTT AAGCTATGGT GCGGTGTCAG GGCCCCTGGC 
AGTGTGGTGC AGTGGGCTAA GGATGGGCTG CTTCTGGGTC CAAACCCGAA GATGCCAGGC 
TTCCCGAGGT ACAGCCTGGA AGGAGATCGT GCTAAAGGCG AGTTCCACCT GCTTATTGAA 
GCCTGTGACC TCAGTGATGA CGCAGAGTAT GAATGCCAAG TCGGCCGCTC AGAGTTGGGT 
CCCGAGCTTG TGTCTCCTAA AGTAATCCTC TCCATTCTAG TTTCCCCCAA GGTGCTTCTG 
TTGACCCCCG AGGCAGGAAG CACAGTGACC TGGGTAGCTG GGCAGGAGTA TGTGGTCACC 
TGTGTGTCTG GAGATGCAAA ACCAGCACCT GACATCACCT TCATCCAGAG TGGACGAACT 
ATATTGGACG TCTCCTCCAA TGTGAATGAG GGATCAGAGG AGAAACTCTG CATCACAGAG 
GCCGAAGCCA GGGTGATACC CCAGAGCTCG GATAACGGGC AGTTACTGGT CTGTGAGGGT 
TCCAACCCAG CTTTGGACAC TCCCATAAAG GCTTCATTCA CCATGAATAT TCTGTTTCCC 
CCAGGACCTC CTGTCATTGA TTGGCCAGGC CTGAATGAGG GGCATGTGAG GGCAGGGGAG 
AACCTGGAGC TGCCCTGCAC AGCCAGAGGT GGCAATCCAC CTGCTACCCT GCAGTGGCTG 
AAGAACGGTA AACCAGTGTC CACAGCCTGG GGCACCGAGC ATGCCCAGGC AGTGGCCCAC 
AGCGTGCTGG TGATGACTGT ACGACCTGAA GACCATGGAG CTCGGCTCAG CTGTCAGTCC 
TACAACAGCG TGTCTGCAGG GACCCAGGAG AGAAGCATCA CACTACAGGT CACCTTTCCC 
CCAAGCGCCA TTACCATCCT GGGATCTGTA TCACAATCGG AGAACAAGAA CGTGACCCTT 
TGCTGCCTGA CCAAGTCCAG TCGCCCACGG GTCCTGCTGC GATGGTGGTT GGGTGGACGG 
CAGCTGCTGC CCACAGATGA GACAGTCATG GATGGCCTGC ATGGTGGCCA CATCTCCATG 
TCCAATCTCA CATTCTTGGT GCGGAGAGAA GACAATGGCC TGCCCCTCAC CTGTGAAGCC 
TTCAGTGACG CCTTCAGCAA GGAGACCTTC AAGAAGTCAC TCACCTTGAA TGTGAAATAC 
CCTGCCCAGA AGCTGTGGAT TGAGGGGCCC CCAGAGGGAC AGTACATCCG GACTGGGACT 
CGGGTGAGGC TGGTATGCTT GGCCATCGGA GGCAACCCAG ACCCCTCCCT CATCTGGTTT 
AAGGATTCAC GTCCGGTGAG CGAGCCCCGG CAGCCCCAGG AGCCCCGGCG TGTGCAGCTG 
GGCAGTGTGG AGAAGTCCGG GAGCACTTTC TCCCGCGAGC TGGTGTTGAT CATAGGTCCG 
CCGGACAACC GAGCCAAGTT CTCCTGCAAG GCGGGTCAGC TCAGTGCGTC TACGCAGCTG 
GTGGTGCAGT TCCCCCCAAC CAACCTGACC ATCCTGGCCA ACTCGTCCGC GCTGCGCCCA 
GGCGACGCCT TGAACTTGAC CTGCGTCAGC ATCAGCAGCA ACCCCCCAGT CAACTTGTCT 
TGGGACAAGG AAGGAGAGAG GCTGGAAGAT GTGGCTGCAA AACCCCAGAG TGCACCGTTC 
AAAGGCTCCG CTGCATCCAG GAGTGTTTTT CTCAGAGTGT CATCCCGAGA CCACGGTCAA 
CGGGTCACCT GCCGGGCCCA CAGCGAGGCA CTCCGTGAAA CCGTGAGCTC CTTCTACCGC 
TTCAATGTGC TGTATCCTCC AGAATTCCTG GGGGAGCAAG TCCGGGCAGT GACCGTGGTG 
GAGCAGGGCC AGGTGCTGCT GCCGGTGTCG GTGTCCGCTA ACCCCGCCCC CGAGGCCTTC 
AACTGGACCT TCCGAGGCTA CCGCCTCAGC CCAGCTGGGG GTCCCCGGCA CCGTATCCTG 
TCTGGAGGGG CTCTGCAGCT GTGGAATGTG ACCCGAGCTG ACGATGGCTT TTATCAGCTG 
CACTGCCAGA ACTCAGAGGG CACCGCTGAG GCGCTGTTGA AGCTGGACGT GCATTATGCT 
CCCACCATCC GTGCCCTCCG GGACCCTACT GAGGTGAATG TTGGGGGTTC TGTGGACATA 
GTCTGCACCG TTGACGCCAA TCCCATCCTC CCAGAGATGT TCAGCTGGGA GAGACTGGGA 
GAAGAAGAGG AGGATCTGAA CCTGGACGAC ATGGAGAAAG TTTCCAAGGG ATCCACGGGG 
CGTCTGCGGA TTCGCCAAGC CAAGCTATCC CAGGCTGGTG CCTACCAGTG CATCGTGGAC 
AATGGGGTGG CTCCTGCAGC CAGAGGACTG GTTCGTCTTG TCGTCCGATT TGCTCCCCAG 
GTGGATCAGC CTACTCCCCT AACAAAAGTG GCTGCCGCTG GGGACAGCAC CAGCTCAGCC 
ACACTGCACT GCCGTGCCCG GGGTGTCCCC AACATCGACT TCACTTGGAC CAAAAACGGG 
GTCCCTCTGG ATCTCCAAGA CCCCAGGTAC ACAGAGCACA GGTACCACCA GGGTGTTGTC 
CACAGCAGCC TCTTGACCAT CGCTAATGTG TCTGCGGCCC AGGACTATGC CCTCTTCAAA 
TGCACGGCCA CCAATGCCCT TGGCTCTGAC CACACCAACA TCCAGCTCGT CAGCATCAGC 
CGCCCTGACC CTCCACTGGG ACTGAAGGTT GTCAGCATAA GCCCTCACTC GGTGGGGCTG 
GAGTGGAAGC CTGGCTTTGA TGGGGGTCTG CCTCAGAGGT TCCAAATCAG GTACGAGGCC 
CTCGAGACCC CAGGATTCCT CCACGTGGAT GTCCTACCTA CACAGGCCAC TACCTTCACG 
CTGACTGGGC TGAAGCCTTC TACACGATAT AGGATCTGGC TGTTGGCCAG CAATGCCCTG 
GGGGACAGTG GATTGACGGA CAAGGGGATC CAGGTCTCCG TCACTACCCC AGGCCCCGAC 
CAGGCTCCTG AAGACACAGA CCACCAGCTG CCCACAGAGC TGCCTCCAGG ACCCCCAAGG 
CTGCCCCTGC TGCCTGTGCT CTTTGCAGTT GGTGGTCTTC TGCTGCTCTC CAATGCCTCC 
TGTGTTGGGG GTCTCCTCTG GCGGAGAAGA CTGAGGCGCC TTGCTGAGGA GATCTCAGAG 
AAGACAGAGG CAGGGTCGGA GGACAGGATC AGGAATGAAT ATGAGGAGAG TCAGTGGACT 
GGGGACCGGG ACACGAGAAG CTCCACGGTT AGCACAGCAG AAGTGGACCC AAATTACTAC 
TCCATGAGGG ACTTCAGCCC CCAGCTTCCC CCAACACTGG AGGAGGTGCT GTATCACCAA 
GGTGCTGAAG GCGAGGACAT GGCCTTCCCC GGACACCTGC ATGATGAAGT GGAGAGAGCC 
TATGGCCCGC CTGGGGCCTG GGGACCCCTC TATGATGAAG TACGAATGGA CCCCTATGAC 
CTTCGCTGGC CTGAGGTCCA GTGTGAGGAT CCGAGGGGAA TCTACGACCA GGTGGCAGCA 
GACATGGATG CTGTGGAAGC TAGCTCTCTG CCGTTTGAGC TGAGGGGACA TCTGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nphs1 ( XM_008759187.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757409.1 Rattus norvegicus NPHS1 nephrin (Nphs1), transcript variant X2, mRNA.

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CloneID	ORa43610
Clone ID Related Accession (Same CDS sequence)	XM_008759187.2
Accession Version	XM_008759187.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	nephrin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759187.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGCTA AGAGAGTCAC TGTCAGAGGT GCCCGGACAA GCCCAATACA CAGAACGTCC 
AGTTTGACTC CCCTGCTGCT CATGGGAATG CTGACCTCAG GCCTGGCCGA GTCGCCAGTC 
CCCACCTCAG CACCTCAAGG CTTCTGGGCT CTGTCTGAAA ACCTGACTGC GGTGGAAGGG 
ACAACAGTTA AGCTATGGTG CGGTGTCAGG GCCCCTGGCA GTGTGGTGCA GTGGGCTAAG 
GATGGGCTGC TTCTGGGTCC AAACCCGAAG ATGCCAGGCT TCCCGAGGTA CAGCCTGGAA 
GGAGATCGTG CTAAAGGCGA GTTCCACCTG CTTATTGAAG CCTGTGACCT CAGTGATGAC 
GCAGAGTATG AATGCCAAGT CGGCCGCTCA GAGTTGGGTC CCGAGCTTGT GTCTCCTAAA 
GTAATCCTCT CCATTCTAGT TTCCCCCAAG GTGCTTCTGT TGACCCCCGA GGCAGGAAGC 
ACAGTGACCT GGGTAGCTGG GCAGGAGTAT GTGGTCACCT GTGTGTCTGG AGATGCAAAA 
CCAGCACCTG ACATCACCTT CATCCAGAGT GGACGAACTA TATTGGACGT CTCCTCCAAT 
GTGAATGAGG GATCAGAGGA GAAACTCTGC ATCACAGAGG CCGAAGCCAG GGTGATACCC 
CAGAGCTCGG ATAACGGGCA GTTACTGGTC TGTGAGGGTT CCAACCCAGC TTTGGACACT 
CCCATAAAGG CTTCATTCAC CATGAATATT CTGTTTCCCC CAGGACCTCC TGTCATTGAT 
TGGCCAGGCC TGAATGAGGG GCATGTGAGG GCAGGGGAGA ACCTGGAGCT GCCCTGCACA 
GCCAGAGGTG GCAATCCACC TGCTACCCTG CAGTGGCTGA AGAACGGTAA ACCAGTGTCC 
ACAGCCTGGG GCACCGAGCA TGCCCAGGCA GTGGCCCACA GCGTGCTGGT GATGACTGTA 
CGACCTGAAG ACCATGGAGC TCGGCTCAGC TGTCAGTCCT ACAACAGCGT GTCTGCAGGG 
ACCCAGGAGA GAAGCATCAC ACTACAGGTC ACCTTTCCCC CAAGCGCCAT TACCATCCTG 
GGATCTGTAT CACAATCGGA GAACAAGAAC GTGACCCTTT GCTGCCTGAC CAAGTCCAGT 
CGCCCACGGG TCCTGCTGCG ATGGTGGTTG GGTGGACGGC AGCTGCTGCC CACAGATGAG 
ACAGTCATGG ATGGCCTGCA TGGTGGCCAC ATCTCCATGT CCAATCTCAC ATTCTTGGTG 
CGGAGAGAAG ACAATGGCCT GCCCCTCACC TGTGAAGCCT TCAGTGACGC CTTCAGCAAG 
GAGACCTTCA AGAAGTCACT CACCTTGAAT GTGAAATACC CTGCCCAGAA GCTGTGGATT 
GAGGGGCCCC CAGAGGGACA GTACATCCGG ACTGGGACTC GGGTGAGGCT GGTATGCTTG 
GCCATCGGAG GCAACCCAGA CCCCTCCCTC ATCTGGTTTA AGGATTCACG TCCGGTGAGC 
GAGCCCCGGC AGCCCCAGGA GCCCCGGCGT GTGCAGCTGG GCAGTGTGGA GAAGTCCGGG 
AGCACTTTCT CCCGCGAGCT GGTGTTGATC ATAGGTCCGC CGGACAACCG AGCCAAGTTC 
TCCTGCAAGG CGGGTCAGCT CAGTGCGTCT ACGCAGCTGG TGGTGCAGTG CGACGCCTTG 
AACTTGACCT GCGTCAGCAT CAGCAGCAAC CCCCCAGTCA ACTTGTCTTG GGACAAGGAA 
GGAGAGAGGC TGGAAGATGT GGCTGCAAAA CCCCAGAGTG CACCGTTCAA AGGCTCCGCT 
GCATCCAGGA GTGTTTTTCT CAGAGTGTCA TCCCGAGACC ACGGTCAACG GGTCACCTGC 
CGGGCCCACA GCGAGGCACT CCGTGAAACC GTGAGCTCCT TCTACCGCTT CAATGTGCTG 
TATCCTCCAG AATTCCTGGG GGAGCAAGTC CGGGCAGTGA CCGTGGTGGA GCAGGGCCAG 
GTGCTGCTGC CGGTGTCGGT GTCCGCTAAC CCCGCCCCCG AGGCCTTCAA CTGGACCTTC 
CGAGGCTACC GCCTCAGCCC AGCTGGGGGT CCCCGGCACC GTATCCTGTC TGGAGGGGCT 
CTGCAGCTGT GGAATGTGAC CCGAGCTGAC GATGGCTTTT ATCAGCTGCA CTGCCAGAAC 
TCAGAGGGCA CCGCTGAGGC GCTGTTGAAG CTGGACGTGC ATTATGCTCC CACCATCCGT 
GCCCTCCGGG ACCCTACTGA GGTGAATGTT GGGGGTTCTG TGGACATAGT CTGCACCGTT 
GACGCCAATC CCATCCTCCC AGAGATGTTC AGCTGGGAGA GACTGGGAGA AGAAGAGGAG 
GATCTGAACC TGGACGACAT GGAGAAAGTT TCCAAGGGAT CCACGGGGCG TCTGCGGATT 
CGCCAAGCCA AGCTATCCCA GGCTGGTGCC TACCAGTGCA TCGTGGACAA TGGGGTGGCT 
CCTGCAGCCA GAGGACTGGT TCGTCTTGTC GTCCGATTTG CTCCCCAGGT GGATCAGCCT 
ACTCCCCTAA CAAAAGTGGC TGCCGCTGGG GACAGCACCA GCTCAGCCAC ACTGCACTGC 
CGTGCCCGGG GTGTCCCCAA CATCGACTTC ACTTGGACCA AAAACGGGGT CCCTCTGGAT 
CTCCAAGACC CCAGGTACAC AGAGCACAGG TACCACCAGG GTGTTGTCCA CAGCAGCCTC 
TTGACCATCG CTAATGTGTC TGCGGCCCAG GACTATGCCC TCTTCAAATG CACGGCCACC 
AATGCCCTTG GCTCTGACCA CACCAACATC CAGCTCGTCA GCATCAGCCG CCCTGACCCT 
CCACTGGGAC TGAAGGTTGT CAGCATAAGC CCTCACTCGG TGGGGCTGGA GTGGAAGCCT 
GGCTTTGATG GGGGTCTGCC TCAGAGGTTC CAAATCAGGT ACGAGGCCCT CGAGACCCCA 
GGATTCCTCC ACGTGGATGT CCTACCTACA CAGGCCACTA CCTTCACGCT GACTGGGCTG 
AAGCCTTCTA CACGATATAG GATCTGGCTG TTGGCCAGCA ATGCCCTGGG GGACAGTGGA 
TTGACGGACA AGGGGATCCA GGTCTCCGTC ACTACCCCAG GCCCCGACCA GGCTCCTGAA 
GACACAGACC ACCAGCTGCC CACAGAGCTG CCTCCAGGAC CCCCAAGGCT GCCCCTGCTG 
CCTGTGCTCT TTGCAGTTGG TGGTCTTCTG CTGCTCTCCA ATGCCTCCTG TGTTGGGGGT 
CTCCTCTGGC GGAGAAGACT GAGGCGCCTT GCTGAGGAGA TCTCAGAGAA GACAGAGGCA 
GGGTCGGAGG ACAGGATCAG GAATGAATAT GAGGAGAGTC AGTGGACTGG GGACCGGGAC 
ACGAGAAGCT CCACGGTTAG CACAGCAGAA GTGGACCCAA ATTACTACTC CATGAGGGAC 
TTCAGCCCCC AGCTTCCCCC AACACTGGAG GAGGTGCTGT ATCACCAAGG TGCTGAAGGC 
GAGGACATGG CCTTCCCCGG ACACCTGCAT GATGAAGTGG AGAGAGCCTA TGGCCCGCCT 
GGGGCCTGGG GACCCCTCTA TGATGAAGTA CGAATGGACC CCTATGACCT TCGCTGGCCT 
GAGGTCCAGT GTGAGGATCC GAGGGGAATC TACGACCAGG TGGCAGCAGA CATGGATGCT 
GTGGAAGCTA GCTCTCTGCC GTTTGAGCTG AGGGGACATC TGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757409.1
CDS	491..4198
Translation	MGAKRVTVRGARTSPIHRTSSLTPLLLMGMLTSGLAESPVPTSAPQGFWALSENLTAVEGTTVKLWCGVRAPGSVVQWAKDGLLLGPNPKMPGFPRYSLEGDRAKGEFHLLIEACDLSDDAEYECQVGRSELGPELVSPKVILSILVSPKVLLLTPEAGSTVTWVAGQEYVVTCVSGDAKPAPDITFIQSGRTILDVSSNVNEGSEEKLCITEAEARVIPQSSDNGQLLVCEGSNPALDTPIKASFTMNILFPPGPPVIDWPGLNEGHVRAGENLELPCTARGGNPPATLQWLKNGKPVSTAWGTEHAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSAGTQERSITLQVTFPPSAITILGSVSQSENKNVTLCCLTKSSRPRVLLRWWLGGRQLLPTDETVMDGLHGGHISMSNLTFLVRREDNGLPLTCEAFSDAFSKETFKKSLTLNVKYPAQKLWIEGPPEGQYIRTGTRVRLVCLAIGGNPDPSLIWFKDSRPVSEPRQPQEPRRVQLGSVEKSGSTFSRELVLIIGPPDNRAKFSCKAGQLSASTQLVVQCDALNLTCVSISSNPPVNLSWDKEGERLEDVAAKPQSAPFKGSAASRSVFLRVSSRDHGQRVTCRAHSEALRETVSSFYRFNVLYPPEFLGEQVRAVTVVEQGQVLLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSGGALQLWNVTRADDGFYQLHCQNSEGTAEALLKLDVHYAPTIRALRDPTEVNVGGSVDIVCTVDANPILPEMFSWERLGEEEEDLNLDDMEKVSKGSTGRLRIRQAKLSQAGAYQCIVDNGVAPAARGLVRLVVRFAPQVDQPTPLTKVAAAGDSTSSATLHCRARGVPNIDFTWTKNGVPLDLQDPRYTEHRYHQGVVHSSLLTIANVSAAQDYALFKCTATNALGSDHTNIQLVSISRPDPPLGLKVVSISPHSVGLEWKPGFDGGLPQRFQIRYEALETPGFLHVDVLPTQATTFTLTGLKPSTRYRIWLLASNALGDSGLTDKGIQVSVTTPGPDQAPEDTDHQLPTELPPGPPRLPLLPVLFAVGGLLLLSNASCVGGLLWRRRLRRLAEEISEKTEAGSEDRIRNEYEESQWTGDRDTRSSTVSTAEVDPNYYSMRDFSPQLPPTLEEVLYHQGAEGEDMAFPGHLHDEVERAYGPPGAWGPLYDEVRMDPYDLRWPEVQCEDPRGIYDQVAADMDAVEASSLPFELRGHLV

Target ORF information:

RefSeq Version	XM_008759187.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NPHS1 nephrin (Nphs1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759187.2

ORF Insert Sequence:

ATGGGCGCTA AGAGAGTCAC TGTCAGAGGT GCCCGGACAA GCCCAATACA CAGAACGTCC 
AGTTTGACTC CCCTGCTGCT CATGGGAATG CTGACCTCAG GCCTGGCCGA GTCGCCAGTC 
CCCACCTCAG CACCTCAAGG CTTCTGGGCT CTGTCTGAAA ACCTGACTGC GGTGGAAGGG 
ACAACAGTTA AGCTATGGTG CGGTGTCAGG GCCCCTGGCA GTGTGGTGCA GTGGGCTAAG 
GATGGGCTGC TTCTGGGTCC AAACCCGAAG ATGCCAGGCT TCCCGAGGTA CAGCCTGGAA 
GGAGATCGTG CTAAAGGCGA GTTCCACCTG CTTATTGAAG CCTGTGACCT CAGTGATGAC 
GCAGAGTATG AATGCCAAGT CGGCCGCTCA GAGTTGGGTC CCGAGCTTGT GTCTCCTAAA 
GTAATCCTCT CCATTCTAGT TTCCCCCAAG GTGCTTCTGT TGACCCCCGA GGCAGGAAGC 
ACAGTGACCT GGGTAGCTGG GCAGGAGTAT GTGGTCACCT GTGTGTCTGG AGATGCAAAA 
CCAGCACCTG ACATCACCTT CATCCAGAGT GGACGAACTA TATTGGACGT CTCCTCCAAT 
GTGAATGAGG GATCAGAGGA GAAACTCTGC ATCACAGAGG CCGAAGCCAG GGTGATACCC 
CAGAGCTCGG ATAACGGGCA GTTACTGGTC TGTGAGGGTT CCAACCCAGC TTTGGACACT 
CCCATAAAGG CTTCATTCAC CATGAATATT CTGTTTCCCC CAGGACCTCC TGTCATTGAT 
TGGCCAGGCC TGAATGAGGG GCATGTGAGG GCAGGGGAGA ACCTGGAGCT GCCCTGCACA 
GCCAGAGGTG GCAATCCACC TGCTACCCTG CAGTGGCTGA AGAACGGTAA ACCAGTGTCC 
ACAGCCTGGG GCACCGAGCA TGCCCAGGCA GTGGCCCACA GCGTGCTGGT GATGACTGTA 
CGACCTGAAG ACCATGGAGC TCGGCTCAGC TGTCAGTCCT ACAACAGCGT GTCTGCAGGG 
ACCCAGGAGA GAAGCATCAC ACTACAGGTC ACCTTTCCCC CAAGCGCCAT TACCATCCTG 
GGATCTGTAT CACAATCGGA GAACAAGAAC GTGACCCTTT GCTGCCTGAC CAAGTCCAGT 
CGCCCACGGG TCCTGCTGCG ATGGTGGTTG GGTGGACGGC AGCTGCTGCC CACAGATGAG 
ACAGTCATGG ATGGCCTGCA TGGTGGCCAC ATCTCCATGT CCAATCTCAC ATTCTTGGTG 
CGGAGAGAAG ACAATGGCCT GCCCCTCACC TGTGAAGCCT TCAGTGACGC CTTCAGCAAG 
GAGACCTTCA AGAAGTCACT CACCTTGAAT GTGAAATACC CTGCCCAGAA GCTGTGGATT 
GAGGGGCCCC CAGAGGGACA GTACATCCGG ACTGGGACTC GGGTGAGGCT GGTATGCTTG 
GCCATCGGAG GCAACCCAGA CCCCTCCCTC ATCTGGTTTA AGGATTCACG TCCGGTGAGC 
GAGCCCCGGC AGCCCCAGGA GCCCCGGCGT GTGCAGCTGG GCAGTGTGGA GAAGTCCGGG 
AGCACTTTCT CCCGCGAGCT GGTGTTGATC ATAGGTCCGC CGGACAACCG AGCCAAGTTC 
TCCTGCAAGG CGGGTCAGCT CAGTGCGTCT ACGCAGCTGG TGGTGCAGTG CGACGCCTTG 
AACTTGACCT GCGTCAGCAT CAGCAGCAAC CCCCCAGTCA ACTTGTCTTG GGACAAGGAA 
GGAGAGAGGC TGGAAGATGT GGCTGCAAAA CCCCAGAGTG CACCGTTCAA AGGCTCCGCT 
GCATCCAGGA GTGTTTTTCT CAGAGTGTCA TCCCGAGACC ACGGTCAACG GGTCACCTGC 
CGGGCCCACA GCGAGGCACT CCGTGAAACC GTGAGCTCCT TCTACCGCTT CAATGTGCTG 
TATCCTCCAG AATTCCTGGG GGAGCAAGTC CGGGCAGTGA CCGTGGTGGA GCAGGGCCAG 
GTGCTGCTGC CGGTGTCGGT GTCCGCTAAC CCCGCCCCCG AGGCCTTCAA CTGGACCTTC 
CGAGGCTACC GCCTCAGCCC AGCTGGGGGT CCCCGGCACC GTATCCTGTC TGGAGGGGCT 
CTGCAGCTGT GGAATGTGAC CCGAGCTGAC GATGGCTTTT ATCAGCTGCA CTGCCAGAAC 
TCAGAGGGCA CCGCTGAGGC GCTGTTGAAG CTGGACGTGC ATTATGCTCC CACCATCCGT 
GCCCTCCGGG ACCCTACTGA GGTGAATGTT GGGGGTTCTG TGGACATAGT CTGCACCGTT 
GACGCCAATC CCATCCTCCC AGAGATGTTC AGCTGGGAGA GACTGGGAGA AGAAGAGGAG 
GATCTGAACC TGGACGACAT GGAGAAAGTT TCCAAGGGAT CCACGGGGCG TCTGCGGATT 
CGCCAAGCCA AGCTATCCCA GGCTGGTGCC TACCAGTGCA TCGTGGACAA TGGGGTGGCT 
CCTGCAGCCA GAGGACTGGT TCGTCTTGTC GTCCGATTTG CTCCCCAGGT GGATCAGCCT 
ACTCCCCTAA CAAAAGTGGC TGCCGCTGGG GACAGCACCA GCTCAGCCAC ACTGCACTGC 
CGTGCCCGGG GTGTCCCCAA CATCGACTTC ACTTGGACCA AAAACGGGGT CCCTCTGGAT 
CTCCAAGACC CCAGGTACAC AGAGCACAGG TACCACCAGG GTGTTGTCCA CAGCAGCCTC 
TTGACCATCG CTAATGTGTC TGCGGCCCAG GACTATGCCC TCTTCAAATG CACGGCCACC 
AATGCCCTTG GCTCTGACCA CACCAACATC CAGCTCGTCA GCATCAGCCG CCCTGACCCT 
CCACTGGGAC TGAAGGTTGT CAGCATAAGC CCTCACTCGG TGGGGCTGGA GTGGAAGCCT 
GGCTTTGATG GGGGTCTGCC TCAGAGGTTC CAAATCAGGT ACGAGGCCCT CGAGACCCCA 
GGATTCCTCC ACGTGGATGT CCTACCTACA CAGGCCACTA CCTTCACGCT GACTGGGCTG 
AAGCCTTCTA CACGATATAG GATCTGGCTG TTGGCCAGCA ATGCCCTGGG GGACAGTGGA 
TTGACGGACA AGGGGATCCA GGTCTCCGTC ACTACCCCAG GCCCCGACCA GGCTCCTGAA 
GACACAGACC ACCAGCTGCC CACAGAGCTG CCTCCAGGAC CCCCAAGGCT GCCCCTGCTG 
CCTGTGCTCT TTGCAGTTGG TGGTCTTCTG CTGCTCTCCA ATGCCTCCTG TGTTGGGGGT 
CTCCTCTGGC GGAGAAGACT GAGGCGCCTT GCTGAGGAGA TCTCAGAGAA GACAGAGGCA 
GGGTCGGAGG ACAGGATCAG GAATGAATAT GAGGAGAGTC AGTGGACTGG GGACCGGGAC 
ACGAGAAGCT CCACGGTTAG CACAGCAGAA GTGGACCCAA ATTACTACTC CATGAGGGAC 
TTCAGCCCCC AGCTTCCCCC AACACTGGAG GAGGTGCTGT ATCACCAAGG TGCTGAAGGC 
GAGGACATGG CCTTCCCCGG ACACCTGCAT GATGAAGTGG AGAGAGCCTA TGGCCCGCCT 
GGGGCCTGGG GACCCCTCTA TGATGAAGTA CGAATGGACC CCTATGACCT TCGCTGGCCT 
GAGGTCCAGT GTGAGGATCC GAGGGGAATC TACGACCAGG TGGCAGCAGA CATGGATGCT 
GTGGAAGCTA GCTCTCTGCC GTTTGAGCTG AGGGGACATC TGGTGTGA