Tkt cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tkt
Entrez Gene ID	64524
Full Name	transketolase
General protein information	Preferred Names transketolase Names transketolase TK
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16p16
Summary	human homolog is a key enzyme in the pentose-phosphate pathway and may be associated with Wernicke-Korsakoff syndrome [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022592.1	NP_072114.1	transketolase
XM_006252661.1	XP_006252723.1	transketolase isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TKT	NP_001244957.1
Danio rerio (zebrafish)	LOC557518	XP_005155944.1
Canis lupus familiaris (dog)	TKT	XP_005632478.1
Bos taurus (cattle)	TKT	NP_001003906.1
Gallus gallus (chicken)	TKT	XP_004944618.1
Pan troglodytes (chimpanzee)	TKT	NP_001009097.1
Mus musculus (house mouse)	Tkt	NP_033414.1
Rattus norvegicus (Norway rat)	Tkt	NP_072114.1
Xenopus tropicalis (tropical clawed frog)	tkt	NP_001015866.1

Gene Ontology
Bibliography

Related articles in PubMed

Downregulation of transketolase activity is related to inhibition of hippocampal progenitor cell proliferation induced by thiamine deficiency.
Zhao Y, Wu Y, Hu H, Cai J, Ning M, Ni X, Zhong C
BioMed research international2014572915(2014)

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W
Immunology and cell biology88(8)851-6(2010 Nov-Dec)

The crystal structure of human transketolase and new insights into its mode of action.
Mitschke L, Parthier C, Schr?der-Tittmann K, Coy J, L?dtke S, Tittmann K
The Journal of biological chemistry285(41)31559-70(2010 Oct)

Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense.
Kesimer M, Scull M, Brighton B, DeMaria G, Burns K, O'Neal W, Pickles RJ, Sheehan JK
FASEB journal : official publication of the Federation of American Societies for Experimental Biology23(6)1858-68(2009 Jun)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results suggest that modulation of transketolase activity might be one of the mechanisms by which thiamine regulates the proliferation of hippocampal progenitor cells.
Title: Downregulation of transketolase activity is related to inhibition of hippocampal progenitor cell proliferation induced by thiamine deficiency.

The following Tkt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tkt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06073	NM_022592.1 Latest version!	Rattus norvegicus transketolase (Tkt), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$384.30 $549.00
ORa37077	XM_006252661.1 Latest version!	Rattus norvegicus transketolase (Tkt), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tkt ( NM_022592.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_072114.1 Rattus norvegicus transketolase (Tkt), mRNA.

Price & Availability↑

CloneID	ORa06073
Clone ID Related Accession (Same CDS sequence)	NM_022592.1
Accession Version	NM_022592.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Rattus norvegicus(Norway rat)
Product	transketolase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U09256.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U09256.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689605, SAMN01906347 [ECO:0000348] ##Evidence-Data-END##

ATGGATCCAG TGAGACAGAT CCAGTGCGAC CGTGAAGGCA AACGCTTTCC GTTCCTCTCA 
GCTCCACCAG CTTCCACGTC CTCGCCCGAC CGCGCCATGG AGGGTTACCA TAAGCCAGAT 
CAGCAGAAGC TCCAGGCCCT GAAGGACACA GCCAATCGCC TGCGCATCAG CTCCATCCAG 
GCCACCACCG CGGCAGGCTC GGGACACCCC ACATCATGCT GCAGCGCTGC CGAGATCATG 
GCTGTCCTGT TTTTCCATAC CATGCGCTAC AAGGCCCTGG ATCCCCGAAA CCCTCACAAT 
GATCGCTTTG TGCTCTCCAA GGGCCATGCA GCTCCCATCT TATATGCAGT CTGGGCTGAA 
GCTGGCTTCC TGCCTGAGGC AGAGCTGCTG AACCTGAGGA AAATCAGCTC TGACTTGGAT 
GGGCATCCTG TCCCGAAACA AGCCTTCACC GATGTGGCCA CTGGCTCCCT GGGCCAGGGG 
CTGGGAGCTG CGTGCGGGAT GGCATACACA GGCAAATACT TCGACAAAGC CAGCTACCGA 
GTCTATTGCA TGCTGGGAGA CGGGGAGGTG TCCGAGGGCT CCGTTTGGGA GGCCATGGCC 
TTCGCTGGAA TTTACAAGCT GGACAACCTC GTTGCCATTT TTGACATCAA CCGTCTGGGC 
CAGAGCGACC CAGCCCCGCT GCAGCACCAA GTGGACGTCT ACCAGAAGCG CTGTGAGGCC 
TTTGGCTGGC ACGCCATCAT CGTGGATGGG CACAGTGTGG AGGAGCTGTG CAAGGCCTTT 
GGTCAGGCCA AGCACCAACC AACAGCCATC ATTGCCAAGA CCTTCAAGGG CCGCGGGATC 
ACAGGGATTG AAGACAAGGA GGCGTGGCAT GGGAAGCCCC TCCCCAAAAA CATGGCTGAG 
CAGATTATCC AGGAGATTTA CAGCCAGGTT CAGAGCAAAA AGAAGATCCT CGCCACGCCC 
CCTCAGGAGG ATGCCCCTTC CGTGGACATT GCCAACATCC GAATGCCTAC CCCACCCAAC 
TACAAAGTGG GGGACAAGAT AGCCACACGG AAAGCCTATG GATTGGCCCT TGCCAAGCTG 
GGCCACGCCA GTGACCGCAT CATCGCCCTG GATGGAGACA CCAAAAATTC CACCTTCTCA 
GAGCTCTTCA AAAAGGAGCA CCCAGACCGT TTCATCGAGT GCTACATTGC TGAGCAGAAC 
ATGGTGAGCA TTGCTGTGGG CTGTGCCACA CGTGACAGGA CAGTGCCCTT CTGCAGCACT 
TTTGCGGCCT TCTTCACACG CGCCTTCGAC CAGATCCGCA TGGCCGCCAT CTCCGAGAGC 
AACATCAACC TTTGTGGCTC CCACTGCGGC GTGTCCATTG GGGAAGACGG GCCCTCGCAG 
ATGGCCCTGG AAGACCTGGC CATGTTTCGG TCGGTCCCTA TGTCCACCGT CTTTTACCCA 
AGTGATGGAG TTGCCACAGA GAAGGCAGTG GAATTAGCAG CCAATACAAA GGGCATCTGC 
TTCATTCGGA CCAGCCGCCC AGAAAATGCC ATTATCTATA GCAACAACGA GGATTTCCAG 
GTTGGCCAAG CCAAGGTGGT CCTGAAGAGC AAGGACGACC AAGTGACAGT GATCGGGGCT 
GGCGTAACTC TGCACGAGGC TCTGGCTGCT GCAGAGATGT TGAAGAAAGA GAAGATCGGT 
GTCCGTGTAC TGGACCCCTT CACCATCAAG CCCCTGGACA AAAAGCTCAT TCTCGACTGT 
GCCAGAGCAA CCAAAGGCAG GATCCTCACC GTGGAGGACC ACTACTATGA AGGTGGCATA 
GGCGAGGCAG TATCTGCTGT GGTAGTGGGC GAACCTGGAG TCACAGTCAC TCGCCTGGCG 
GTCAGCCAAG TACCACGAAG TGGGAAGCCA GCTGAGCTGC TGAAGATGTT TGGTATTGAC 
AAAGACGCCA TTGTGCAAGC TGTGAAGGGC CTTGTCACCA AGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_072114.1
CDS	16..1983
Translation	MDPVRQIQCDREGKRFPFLSAPPASTSSPDRAMEGYHKPDQQKLQALKDTANRLRISSIQATTAAGSGHPTSCCSAAEIMAVLFFHTMRYKALDPRNPHNDRFVLSKGHAAPILYAVWAEAGFLPEAELLNLRKISSDLDGHPVPKQAFTDVATGSLGQGLGAACGMAYTGKYFDKASYRVYCMLGDGEVSEGSVWEAMAFAGIYKLDNLVAIFDINRLGQSDPAPLQHQVDVYQKRCEAFGWHAIIVDGHSVEELCKAFGQAKHQPTAIIAKTFKGRGITGIEDKEAWHGKPLPKNMAEQIIQEIYSQVQSKKKILATPPQEDAPSVDIANIRMPTPPNYKVGDKIATRKAYGLALAKLGHASDRIIALDGDTKNSTFSELFKKEHPDRFIECYIAEQNMVSIAVGCATRDRTVPFCSTFAAFFTRAFDQIRMAAISESNINLCGSHCGVSIGEDGPSQMALEDLAMFRSVPMSTVFYPSDGVATEKAVELAANTKGICFIRTSRPENAIIYSNNEDFQVGQAKVVLKSKDDQVTVIGAGVTLHEALAAAEMLKKEKIGVRVLDPFTIKPLDKKLILDCARATKGRILTVEDHYYEGGIGEAVSAVVVGEPGVTVTRLAVSQVPRSGKPAELLKMFGIDKDAIVQAVKGLVTKG

Target ORF information:

RefSeq Version	NM_022592.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus transketolase (Tkt), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022592.1

ORF Insert Sequence:

ATGGATCCAG TGAGACAGAT CCAGTGCGAC CGTGAAGGCA AACGCTTTCC GTTCCTCTCA 
GCTCCACCAG CTTCCACGTC CTCGCCCGAC CGCGCCATGG AGGGTTACCA TAAGCCAGAT 
CAGCAGAAGC TCCAGGCCCT GAAGGACACA GCCAATCGCC TGCGCATCAG CTCCATCCAG 
GCCACCACCG CGGCAGGCTC GGGACACCCC ACATCATGCT GCAGCGCTGC CGAGATCATG 
GCTGTCCTGT TTTTCCATAC CATGCGCTAC AAGGCCCTGG ATCCCCGAAA CCCTCACAAT 
GATCGCTTTG TGCTCTCCAA GGGCCATGCA GCTCCCATCT TATATGCAGT CTGGGCTGAA 
GCTGGCTTCC TGCCTGAGGC AGAGCTGCTG AACCTGAGGA AAATCAGCTC TGACTTGGAT 
GGGCATCCTG TCCCGAAACA AGCCTTCACC GATGTGGCCA CTGGCTCCCT GGGCCAGGGG 
CTGGGAGCTG CGTGCGGGAT GGCATACACA GGCAAATACT TCGACAAAGC CAGCTACCGA 
GTCTATTGCA TGCTGGGAGA CGGGGAGGTG TCCGAGGGCT CCGTTTGGGA GGCCATGGCC 
TTCGCTGGAA TTTACAAGCT GGACAACCTC GTTGCCATTT TTGACATCAA CCGTCTGGGC 
CAGAGCGACC CAGCCCCGCT GCAGCACCAA GTGGACGTCT ACCAGAAGCG CTGTGAGGCC 
TTTGGCTGGC ACGCCATCAT CGTGGATGGG CACAGTGTGG AGGAGCTGTG CAAGGCCTTT 
GGTCAGGCCA AGCACCAACC AACAGCCATC ATTGCCAAGA CCTTCAAGGG CCGCGGGATC 
ACAGGGATTG AAGACAAGGA GGCGTGGCAT GGGAAGCCCC TCCCCAAAAA CATGGCTGAG 
CAGATTATCC AGGAGATTTA CAGCCAGGTT CAGAGCAAAA AGAAGATCCT CGCCACGCCC 
CCTCAGGAGG ATGCCCCTTC CGTGGACATT GCCAACATCC GAATGCCTAC CCCACCCAAC 
TACAAAGTGG GGGACAAGAT AGCCACACGG AAAGCCTATG GATTGGCCCT TGCCAAGCTG 
GGCCACGCCA GTGACCGCAT CATCGCCCTG GATGGAGACA CCAAAAATTC CACCTTCTCA 
GAGCTCTTCA AAAAGGAGCA CCCAGACCGT TTCATCGAGT GCTACATTGC TGAGCAGAAC 
ATGGTGAGCA TTGCTGTGGG CTGTGCCACA CGTGACAGGA CAGTGCCCTT CTGCAGCACT 
TTTGCGGCCT TCTTCACACG CGCCTTCGAC CAGATCCGCA TGGCCGCCAT CTCCGAGAGC 
AACATCAACC TTTGTGGCTC CCACTGCGGC GTGTCCATTG GGGAAGACGG GCCCTCGCAG 
ATGGCCCTGG AAGACCTGGC CATGTTTCGG TCGGTCCCTA TGTCCACCGT CTTTTACCCA 
AGTGATGGAG TTGCCACAGA GAAGGCAGTG GAATTAGCAG CCAATACAAA GGGCATCTGC 
TTCATTCGGA CCAGCCGCCC AGAAAATGCC ATTATCTATA GCAACAACGA GGATTTCCAG 
GTTGGCCAAG CCAAGGTGGT CCTGAAGAGC AAGGACGACC AAGTGACAGT GATCGGGGCT 
GGCGTAACTC TGCACGAGGC TCTGGCTGCT GCAGAGATGT TGAAGAAAGA GAAGATCGGT 
GTCCGTGTAC TGGACCCCTT CACCATCAAG CCCCTGGACA AAAAGCTCAT TCTCGACTGT 
GCCAGAGCAA CCAAAGGCAG GATCCTCACC GTGGAGGACC ACTACTATGA AGGTGGCATA 
GGCGAGGCAG TATCTGCTGT GGTAGTGGGC GAACCTGGAG TCACAGTCAC TCGCCTGGCG 
GTCAGCCAAG TACCACGAAG TGGGAAGCCA GCTGAGCTGC TGAAGATGTT TGGTATTGAC 
AAAGACGCCA TTGTGCAAGC TGTGAAGGGC CTTGTCACCA AGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tkt ( XM_006252661.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006252723.1 Rattus norvegicus transketolase (Tkt), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa37077
Clone ID Related Accession (Same CDS sequence)	XM_006252661.1
Accession Version	XM_006252661.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	transketolase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGTT ACCATAAGCC AGATCAGCAG AAGCTCCAGG CCCTGAAGGA CACAGCCAAT 
CGCCTGCGCA TCAGCTCCAT CCAGGCCACC ACCGCGGCAG GCTCGGGACA CCCCACATCA 
TGCTGCAGCG CTGCCGAGAT CATGGCTGTC CTGTTTTTCC ATACCATGCG CTACAAGGCC 
CTGGATCCCC GAAACCCTCA CAATGATCGC TTTGTGCTCT CCAAGGGCCA TGCAGCTCCC 
ATCTTATATG CAGTCTGGGC TGAAGCTGGC TTCCTGCCTG AGGCAGAGCT GCTGAACCTG 
AGGAAAATCA GCTCTGACTT GGATGGGCAT CCTGTCCCGA AACAAGCCTT CACCGATGTG 
GCCACTGGCT CCCTGGGCCA GGGGCTGGGA GCTGCGTGCG GGATGGCATA CACAGGCAAA 
TACTTCGACA AAGCCAGCTA CCGAGTCTAT TGCATGCTGG GAGACGGGGA GGTGTCCGAG 
GGCTCCGTTT GGGAGGCCAT GGCCTTCGCT GGAATTTACA AGCTGGACAA CCTCGTTGCC 
ATTTTTGACA TCAACCGTCT GGGCCAGAGC GACCCAGCCC CGCTGCAGCA CCAAGTGGAC 
GTCTACCAGA AGCGCTGTGA GGCCTTTGGC TGGCATGCCA TCATCGTGGA TGGGCACAGT 
GTGGAGGAGC TGTGCAAGGC CTTTGGTCAG GCCAAGCACC AACCAACAGC CATCATTGCC 
AAGACCTTCA AGGGCCGCGG GATCACAGGG ATTGAAGACA AGGAGGCGTG GCATGGGAAG 
CCCCTCCCCA AAAACATGGC TGAGCAGATT ATCCAGGAGA TTTACAGCCA GGTTCAGAGC 
AAAAAGAAGA TCCTCGCCAC GCCCCCTCAG GAGGATGCCC CTTCCGTGGA CATTGCCAAC 
ATCCGAATGC CTACCCCACC CAACTACAAA GTGGGGGACA AGATAGCCAC ACGGAAAGCC 
TATGGATTGG CCCTTGCCAA GCTGGGCCAC GCCAGTGACC GCATCATCGC CCTGGATGGA 
GACACCAAAA ATTCCACCTT CTCAGAGCTC TTCAAAAAGG AGCACCCAGA CCGTTTCATC 
GAGTGCTACA TTGCTGAGCA GAACATGGTG AGCATTGCTG TGGGCTGTGC CACACGTGAC 
AGGACAGTGC CCTTCTGCAG CACTTTTGCG GCCTTCTTCA CACGCGCCTT CGACCAGATC 
CGCATGGCCG CCATCTCCGA GAGCAACATC AACCTCTGTG GCTCCCATTG CGGCGTGTCC 
ATTGGGGAAG ACGGGCCCTC GCAGATGGCC CTGGAAGACC TGGCCATGTT TCGGTCGGTC 
CCTATGTCCA CCGTCTTTTA CCCAAGTGAT GGAGTTGCCA CAGAGAAGGC AGTGGAATTA 
GCAGCCAATA CAAAGGGCAT CTGCTTCATT CGGACCAGCC GCCCAGAAAA TGCCATTATC 
TATAGCAACA ACGAGGATTT CCAGGTTGGC CAAGCCAAGG TGGTCCTGAA GAGCAAGGAC 
GACCAAGTGA CGGTGATCGG GGCTGGCGTA ACTCTGCACG AGGCTCTGGC TGCTGCAGAG 
ATGTTGAAGA AAGAGAAGAT CGGCGTCCGT GTACTGGACC CCTTCACCAT CAAGCCCCTG 
GACAAAAAGC TCATTCTCGA CTGTGCCAGA GCAACCAAAG GCAGGATCCT CACCGTGGAG 
GACCACTACT ATGAAGGTGG CATAGGCGAG GCAGTATCTG CTGCGGTAGT GGGCGAACCT 
GGAGTCACAG TCACTCGCCT GGCGGTCAGC CAAGTACCAC GAAGTGGGAA GCCAGCTGAG 
CTGCTGAAGA TGTTTGGTAT TGACAAAGAC GCCATTGTGC AAGCTGTGAA GGGCCTTGTC 
ACCAAGGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006252723.1
CDS	144..2015
Translation	MEGYHKPDQQKLQALKDTANRLRISSIQATTAAGSGHPTSCCSAAEIMAVLFFHTMRYKALDPRNPHNDRFVLSKGHAAPILYAVWAEAGFLPEAELLNLRKISSDLDGHPVPKQAFTDVATGSLGQGLGAACGMAYTGKYFDKASYRVYCMLGDGEVSEGSVWEAMAFAGIYKLDNLVAIFDINRLGQSDPAPLQHQVDVYQKRCEAFGWHAIIVDGHSVEELCKAFGQAKHQPTAIIAKTFKGRGITGIEDKEAWHGKPLPKNMAEQIIQEIYSQVQSKKKILATPPQEDAPSVDIANIRMPTPPNYKVGDKIATRKAYGLALAKLGHASDRIIALDGDTKNSTFSELFKKEHPDRFIECYIAEQNMVSIAVGCATRDRTVPFCSTFAAFFTRAFDQIRMAAISESNINLCGSHCGVSIGEDGPSQMALEDLAMFRSVPMSTVFYPSDGVATEKAVELAANTKGICFIRTSRPENAIIYSNNEDFQVGQAKVVLKSKDDQVTVIGAGVTLHEALAAAEMLKKEKIGVRVLDPFTIKPLDKKLILDCARATKGRILTVEDHYYEGGIGEAVSAAVVGEPGVTVTRLAVSQVPRSGKPAELLKMFGIDKDAIVQAVKGLVTKG

Target ORF information:

RefSeq Version	XM_006252661.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus transketolase (Tkt), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006252661.1

ORF Insert Sequence:

ATGGAGGGTT ACCATAAGCC AGATCAGCAG AAGCTCCAGG CCCTGAAGGA CACAGCCAAT 
CGCCTGCGCA TCAGCTCCAT CCAGGCCACC ACCGCGGCAG GCTCGGGACA CCCCACATCA 
TGCTGCAGCG CTGCCGAGAT CATGGCTGTC CTGTTTTTCC ATACCATGCG CTACAAGGCC 
CTGGATCCCC GAAACCCTCA CAATGATCGC TTTGTGCTCT CCAAGGGCCA TGCAGCTCCC 
ATCTTATATG CAGTCTGGGC TGAAGCTGGC TTCCTGCCTG AGGCAGAGCT GCTGAACCTG 
AGGAAAATCA GCTCTGACTT GGATGGGCAT CCTGTCCCGA AACAAGCCTT CACCGATGTG 
GCCACTGGCT CCCTGGGCCA GGGGCTGGGA GCTGCGTGCG GGATGGCATA CACAGGCAAA 
TACTTCGACA AAGCCAGCTA CCGAGTCTAT TGCATGCTGG GAGACGGGGA GGTGTCCGAG 
GGCTCCGTTT GGGAGGCCAT GGCCTTCGCT GGAATTTACA AGCTGGACAA CCTCGTTGCC 
ATTTTTGACA TCAACCGTCT GGGCCAGAGC GACCCAGCCC CGCTGCAGCA CCAAGTGGAC 
GTCTACCAGA AGCGCTGTGA GGCCTTTGGC TGGCATGCCA TCATCGTGGA TGGGCACAGT 
GTGGAGGAGC TGTGCAAGGC CTTTGGTCAG GCCAAGCACC AACCAACAGC CATCATTGCC 
AAGACCTTCA AGGGCCGCGG GATCACAGGG ATTGAAGACA AGGAGGCGTG GCATGGGAAG 
CCCCTCCCCA AAAACATGGC TGAGCAGATT ATCCAGGAGA TTTACAGCCA GGTTCAGAGC 
AAAAAGAAGA TCCTCGCCAC GCCCCCTCAG GAGGATGCCC CTTCCGTGGA CATTGCCAAC 
ATCCGAATGC CTACCCCACC CAACTACAAA GTGGGGGACA AGATAGCCAC ACGGAAAGCC 
TATGGATTGG CCCTTGCCAA GCTGGGCCAC GCCAGTGACC GCATCATCGC CCTGGATGGA 
GACACCAAAA ATTCCACCTT CTCAGAGCTC TTCAAAAAGG AGCACCCAGA CCGTTTCATC 
GAGTGCTACA TTGCTGAGCA GAACATGGTG AGCATTGCTG TGGGCTGTGC CACACGTGAC 
AGGACAGTGC CCTTCTGCAG CACTTTTGCG GCCTTCTTCA CACGCGCCTT CGACCAGATC 
CGCATGGCCG CCATCTCCGA GAGCAACATC AACCTCTGTG GCTCCCATTG CGGCGTGTCC 
ATTGGGGAAG ACGGGCCCTC GCAGATGGCC CTGGAAGACC TGGCCATGTT TCGGTCGGTC 
CCTATGTCCA CCGTCTTTTA CCCAAGTGAT GGAGTTGCCA CAGAGAAGGC AGTGGAATTA 
GCAGCCAATA CAAAGGGCAT CTGCTTCATT CGGACCAGCC GCCCAGAAAA TGCCATTATC 
TATAGCAACA ACGAGGATTT CCAGGTTGGC CAAGCCAAGG TGGTCCTGAA GAGCAAGGAC 
GACCAAGTGA CGGTGATCGG GGCTGGCGTA ACTCTGCACG AGGCTCTGGC TGCTGCAGAG 
ATGTTGAAGA AAGAGAAGAT CGGCGTCCGT GTACTGGACC CCTTCACCAT CAAGCCCCTG 
GACAAAAAGC TCATTCTCGA CTGTGCCAGA GCAACCAAAG GCAGGATCCT CACCGTGGAG 
GACCACTACT ATGAAGGTGG CATAGGCGAG GCAGTATCTG CTGCGGTAGT GGGCGAACCT 
GGAGTCACAG TCACTCGCCT GGCGGTCAGC CAAGTACCAC GAAGTGGGAA GCCAGCTGAG 
CTGCTGAAGA TGTTTGGTAT TGACAAAGAC GCCATTGTGC AAGCTGTGAA GGGCCTTGTC 
ACCAAGGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Downregulation of transketolase activity is related to inhibition of hippocampal progenitor cell proliferation induced by thiamine deficiency.
BioMed research international2014572915(2014)
Zhao Y,Wu Y,Hu H,Cai J,Ning M,Ni X,Zhong C

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Immunology and cell biology88(8)851-6(2010 Nov-Dec)
Buschow SI,van Balkom BW,Aalberts M,Heck AJ,Wauben M,Stoorvogel W

The crystal structure of human transketolase and new insights into its mode of action.
The Journal of biological chemistry285(41)31559-70(2010 Oct)
Mitschke L,Parthier C,Schr?der-Tittmann K,Coy J,L?dtke S,Tittmann K

Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology23(6)1858-68(2009 Jun)
Kesimer M,Scull M,Brighton B,DeMaria G,Burns K,O'Neal W,Pickles RJ,Sheehan JK

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

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Tkt cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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