Fzd2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fzd2
Entrez Gene ID	64512
Full Name	frizzled class receptor 2
Synonyms	Fz-10,Fzd10
General protein information	Preferred Names frizzled class receptor 2 Names frizzled-2 Drosophila polarity gene (frizzled) homologue Drosophila polarity gene homolog 2 frizzled family receptor 2 fz-2 rFz2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.1
Summary	G-protein coupled receptor that binds Wnt proteins and induces intracellular calcium release; also mediates signaling via inhibition of cGMP [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_172035.1	NP_742032.1	frizzled-2 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FZD2	XP_003315471.1
Canis lupus familiaris (dog)	FZD2	XP_548060.2
Bos taurus (cattle)	FZD2	XP_003587503.1
Gallus gallus (chicken)	FZD2	NP_989553.1
Danio rerio (zebrafish)	fzd2	NP_571215.1
Xenopus tropicalis (tropical clawed frog)	fzd2	NP_001008449.1
Mus musculus (house mouse)	Fzd2	NP_065256.1
	FZD2	XP_001101358.2
Homo sapiens (human)	FZD2	NP_001457.1
Rattus norvegicus (Norway rat)	Fzd2	NP_742032.1

Gene Ontology
Bibliography

Related articles in PubMed

The Involvement of the Decrease of Astrocytic Wnt5a in the Cognitive Decline in Minimal Hepatic Encephalopathy.
Ding S, Xu Z, Yang J, Liu L, Huang X, Wang X, Zhuge Q
Molecular neurobiology54(10)7949-7963(2017 Dec)

Mapping of Wnt-Frizzled interactions by multiplex CRISPR targeting of receptor gene families.
Voloshanenko O, Gmach P, Winter J, Kranz D, Boutros M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(11)4832-4844(2017 Nov)

Resveratrol attenuates hypoxia/reoxygenation?induced Ca2+ overload by inhibiting the Wnt5a/Frizzled?2 pathway in rat H9c2 cells.
Wu X, Zhou S, Zhu N, Wang X, Jin W, Song X, Chen A
Molecular medicine reports10(5)2542-8(2014 Nov)

Secreted Frizzled-related protein potentiation versus inhibition of Wnt3a/?-catenin signaling.
Xavier CP, Melikova M, Chuman Y, ?ren A, Baljinnyam B, Rubin JS
Cellular signalling26(1)94-101(2014 Jan)

Suppression of Frizzled-2-mediated Wnt/Ca?? signaling significantly attenuates intracellular calcium accumulation in vitro and in a rat model of traumatic brain injury.
Niu LJ, Xu RX, Zhang P, Du MX, Jiang XD
Neuroscience21319-28(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

H9c2 cell treatment with resveratrol significantly reduces the expression of Frizzled2 and Wnt5a.
Title: Resveratrol attenuates hypoxia/reoxygenation?induced Ca2+ overload by inhibiting the Wnt5a/Frizzled?2 pathway in rat H9c2 cells.

upon binding to Wnt5a, increased Frizzled-2 expression after hypoxia/reoxygenation treatment activated intracellular calcium release in H9c2 cells
Title: Suppression of rat Frizzled-2 attenuates hypoxia/reoxygenation-induced Ca2+ accumulation in rat H9c2 cells.

These findings demonstrated that the Wnt5a/Fzd2 signaling pathway contributed to increasing intracellular Ca(2) in nerve cells under physiological and pathological conditions
Title: Suppression of Frizzled-2-mediated Wnt/Ca?? signaling significantly attenuates intracellular calcium accumulation in vitro and in a rat model of traumatic brain injury.

Highly upregulated expression of Wnt5a and its receptor frizzled 2 implicates this pathway in differentiation of quiescent hepatic stellate cells into myofibroblasts.
Title: Gene expression profile of quiescent and activated rat hepatic stellate cells implicates Wnt signaling pathway in activation.

role of phosphodiesterase and of cGMP in Frizzled-2 signaling
Title: Signaling of rat Frizzled-2 through phosphodiesterase and cyclic GMP.

The following Fzd2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fzd2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13778	NM_172035.1 Latest version!	Rattus norvegicus frizzled class receptor 2 (Fzd2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fzd2 ( NM_172035.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_742032.1 Rattus norvegicus frizzled class receptor 2 (Fzd2), mRNA.

Price & Availability↑

CloneID	ORa13778
Clone ID Related Accession (Same CDS sequence)	NM_172035.1
Accession Version	NM_172035.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	frizzled-2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L02530.1. On Jan 12, 2005 this sequence version replaced XM_346408.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L02530.1 [ECO:0000332] ##Evidence-Data-END##

ATGCGGGCCC GCAGCGCCCT GCCCCGCAGC GCCCTGCCCC GCCTGCTGCT GCCACTGCTG 
CTGCTGCCGG CTGCCGGGCC GGCTCAGTTC CACGGGGAGA AGGGCATCTC CATCCCGGAC 
CACGGCTTCT GCCAGCCCAT CTCCATCCCG CTGTGCACGG ACATCGCCTA CAACCAGACC 
ATCATGCCCA ACCTTCTTGG GCACACGAAC CAAGAGGACG CGGGCCTGGA GGTGCATCAA 
TTCTACCCGC TGGTGAAGGT GCAGTGCTCG CCCGAGCTGC GCTTCTTCCT GTGCTCCATG 
TACGCTCCGG TGTGCACGGT GCTGGAGCAG GCCATCCCGC CGTGCCGCTC CATCTGCGAA 
CGCGCGCGCC AAGGCTGCGA GGCGCTCATG AACAAGTTCG GCTTCCAGTG GCCCGAGCGC 
CTCCGCTGCG AGCATTTCCC GCGTCACGGC GCGGAGCAGA TCTGCGTGGG CCAGAACCAC 
TCCGAGGACG GAACTCCTGC GCTACTCACC ACCGCGCCAC CGTCTGGGCT GCAGCCTGGC 
GCTGGTGGCA CCCCGGGCGG CCCTGGCGGT GGTGGCGCGC CCCCGCGCTA CGCCACTCTG 
GAGCACCCTT TCCACTGTCC CCGCGTCCTC AAGGTGCCGT CCTATCTCAG CTATAAGTTT 
CTGGGTGAGC GCGATTGTGC CGCGCCCTGC GAGCCTGCAC GGCCCGACGG CTCCATGTTC 
TTCTCGCACC ACCACACTCG TTTTGCCCGT CTCTGGATCC TCACATGGTC GGTGCTGTGC 
TGCGCTTCTA CTTTCTTCAC GGTCACCACC TCTTTAGTGG CCATGCAGCG ATTCCGCTAC 
CCAGAGCGGC CCATCATCTT CCTGTCCGGT TGCTACACCA TGGTGTCAGT GGCCTACATT 
GCGGGCTTCG TGCTCCAGGA GCGCGTGGTG TGCAACGAGC GCTTCTCTGA GGACGGTTAT 
CGCACGGTGG GGCAGGGCAC TAAGAAAGAA GGCTGTACTA TACTCTTCAT GATGCTCTAC 
TTCTTCAGTA TGGCCAGCTC CATCTGGTGG GTGATTCTGT CCCTCACCTG GTTCCTGGCA 
GCCGGTATGA AGTGGGGCCA CGCGGCCATC GAGGCCAATT CGCAGTACTT CCACCTGGCC 
GCCTGGGCGG TGCCGGCCGT CAAAACCATC ACCATCCTGG CCATGGGCCA GATCGACGGC 
GACCTGCTGA GCGGCGTGTG CTTCGTGGGC CTCAACAGGC TGGACCCGCT GCGAGGCTTC 
GTGCTGGCGC CGCTCTTCGT GTACCTGTTC ATCGGCACAT CCTTCCTGCT GGCGGGCTTC 
GTGTCACTCT TCCGCATCCG CACCATCATG AAGCACGACG GCACCAAGAC GGAGCCGCTG 
GAGAGGCTCA TGGTGCGTAT CGGCGTCTTC TCCGTGCTCT ACACCGTACC GGCCACCATC 
GTCATCGCCT GCTACTTCTA TGAGCAGGCC TTCCGCGAGC ACTGGGAGCG CTCGTGGGTA 
AGCCAGCACT GCAAGAGCCT AGCCATCCCC TGCCCGGCCC ACTACACGCC CCGCACGTCG 
CCCGACTTCA CAGTCTACAT GATCAAATAC CTCATGACGC TCATCGTGGG CATCACGTCG 
GGCTTCTGGA TCTGGTCCGG CAAGACGCTG CACTCGTGGA GGAAGTTCTA CACGCGTCTC 
ACCAACAGCC GGCATGGAGA GACCACCGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_742032.1
CDS	79..1791
Translation	MRARSALPRSALPRLLLPLLLLPAAGPAQFHGEKGISIPDHGFCQPISIPLCTDIAYNQTIMPNLLGHTNQEDAGLEVHQFYPLVKVQCSPELRFFLCSMYAPVCTVLEQAIPPCRSICERARQGCEALMNKFGFQWPERLRCEHFPRHGAEQICVGQNHSEDGTPALLTTAPPSGLQPGAGGTPGGPGGGGAPPRYATLEHPFHCPRVLKVPSYLSYKFLGERDCAAPCEPARPDGSMFFSHHHTRFARLWILTWSVLCCASTFFTVTTSLVAMQRFRYPERPIIFLSGCYTMVSVAYIAGFVLQERVVCNERFSEDGYRTVGQGTKKEGCTILFMMLYFFSMASSIWWVILSLTWFLAAGMKWGHAAIEANSQYFHLAAWAVPAVKTITILAMGQIDGDLLSGVCFVGLNRLDPLRGFVLAPLFVYLFIGTSFLLAGFVSLFRIRTIMKHDGTKTEPLERLMVRIGVFSVLYTVPATIVIACYFYEQAFREHWERSWVSQHCKSLAIPCPAHYTPRTSPDFTVYMIKYLMTLIVGITSGFWIWSGKTLHSWRKFYTRLTNSRHGETTV

Target ORF information:

RefSeq Version	NM_172035.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus frizzled class receptor 2 (Fzd2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172035.1

ORF Insert Sequence:

ATGCGGGCCC GCAGCGCCCT GCCCCGCAGC GCCCTGCCCC GCCTGCTGCT GCCACTGCTG 
CTGCTGCCGG CTGCCGGGCC GGCTCAGTTC CACGGGGAGA AGGGCATCTC CATCCCGGAC 
CACGGCTTCT GCCAGCCCAT CTCCATCCCG CTGTGCACGG ACATCGCCTA CAACCAGACC 
ATCATGCCCA ACCTTCTTGG GCACACGAAC CAAGAGGACG CGGGCCTGGA GGTGCATCAA 
TTCTACCCGC TGGTGAAGGT GCAGTGCTCG CCCGAGCTGC GCTTCTTCCT GTGCTCCATG 
TACGCTCCGG TGTGCACGGT GCTGGAGCAG GCCATCCCGC CGTGCCGCTC CATCTGCGAA 
CGCGCGCGCC AAGGCTGCGA GGCGCTCATG AACAAGTTCG GCTTCCAGTG GCCCGAGCGC 
CTCCGCTGCG AGCATTTCCC GCGTCACGGC GCGGAGCAGA TCTGCGTGGG CCAGAACCAC 
TCCGAGGACG GAACTCCTGC GCTACTCACC ACCGCGCCAC CGTCTGGGCT GCAGCCTGGC 
GCTGGTGGCA CCCCGGGCGG CCCTGGCGGT GGTGGCGCGC CCCCGCGCTA CGCCACTCTG 
GAGCACCCTT TCCACTGTCC CCGCGTCCTC AAGGTGCCGT CCTATCTCAG CTATAAGTTT 
CTGGGTGAGC GCGATTGTGC CGCGCCCTGC GAGCCTGCAC GGCCCGACGG CTCCATGTTC 
TTCTCGCACC ACCACACTCG TTTTGCCCGT CTCTGGATCC TCACATGGTC GGTGCTGTGC 
TGCGCTTCTA CTTTCTTCAC GGTCACCACC TCTTTAGTGG CCATGCAGCG ATTCCGCTAC 
CCAGAGCGGC CCATCATCTT CCTGTCCGGT TGCTACACCA TGGTGTCAGT GGCCTACATT 
GCGGGCTTCG TGCTCCAGGA GCGCGTGGTG TGCAACGAGC GCTTCTCTGA GGACGGTTAT 
CGCACGGTGG GGCAGGGCAC TAAGAAAGAA GGCTGTACTA TACTCTTCAT GATGCTCTAC 
TTCTTCAGTA TGGCCAGCTC CATCTGGTGG GTGATTCTGT CCCTCACCTG GTTCCTGGCA 
GCCGGTATGA AGTGGGGCCA CGCGGCCATC GAGGCCAATT CGCAGTACTT CCACCTGGCC 
GCCTGGGCGG TGCCGGCCGT CAAAACCATC ACCATCCTGG CCATGGGCCA GATCGACGGC 
GACCTGCTGA GCGGCGTGTG CTTCGTGGGC CTCAACAGGC TGGACCCGCT GCGAGGCTTC 
GTGCTGGCGC CGCTCTTCGT GTACCTGTTC ATCGGCACAT CCTTCCTGCT GGCGGGCTTC 
GTGTCACTCT TCCGCATCCG CACCATCATG AAGCACGACG GCACCAAGAC GGAGCCGCTG 
GAGAGGCTCA TGGTGCGTAT CGGCGTCTTC TCCGTGCTCT ACACCGTACC GGCCACCATC 
GTCATCGCCT GCTACTTCTA TGAGCAGGCC TTCCGCGAGC ACTGGGAGCG CTCGTGGGTA 
AGCCAGCACT GCAAGAGCCT AGCCATCCCC TGCCCGGCCC ACTACACGCC CCGCACGTCG 
CCCGACTTCA CAGTCTACAT GATCAAATAC CTCATGACGC TCATCGTGGG CATCACGTCG 
GGCTTCTGGA TCTGGTCCGG CAAGACGCTG CACTCGTGGA GGAAGTTCTA CACGCGTCTC 
ACCAACAGCC GGCATGGAGA GACCACCGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Involvement of the Decrease of Astrocytic Wnt5a in the Cognitive Decline in Minimal Hepatic Encephalopathy.
Molecular neurobiology54(10)7949-7963(2017 Dec)
Ding S,Xu Z,Yang J,Liu L,Huang X,Wang X,Zhuge Q

Mapping of Wnt-Frizzled interactions by multiplex CRISPR targeting of receptor gene families.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(11)4832-4844(2017 Nov)
Voloshanenko O,Gmach P,Winter J,Kranz D,Boutros M

Resveratrol attenuates hypoxia/reoxygenation?induced Ca2+ overload by inhibiting the Wnt5a/Frizzled?2 pathway in rat H9c2 cells.
Molecular medicine reports10(5)2542-8(2014 Nov)
Wu X,Zhou S,Zhu N,Wang X,Jin W,Song X,Chen A

Secreted Frizzled-related protein potentiation versus inhibition of Wnt3a/?-catenin signaling.
Cellular signalling26(1)94-101(2014 Jan)
Xavier CP,Melikova M,Chuman Y,?ren A,Baljinnyam B,Rubin JS

Suppression of Frizzled-2-mediated Wnt/Ca?? signaling significantly attenuates intracellular calcium accumulation in vitro and in a rat model of traumatic brain injury.
Neuroscience21319-28(2012 Jun)
Niu LJ,Xu RX,Zhang P,Du MX,Jiang XD