Aplp2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Aplp2
Entrez Gene ID	64312
Full Name	amyloid beta precursor like protein 2
Synonyms	APLP-2,Apph,WALPLP2
General protein information	Preferred Names amyloid beta precursor like protein 2 Names amyloid-like protein 2 Amyloid protein precursor-like protein 2 amyloid beta (A4) precursor-like protein 2 amyloid beta (A4) precursor-like protein 2-like sperm membrane protein (YWK-II)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q13
Summary	may contribute to membrane cytoplasmic junctions in sperm membranes [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012906.1	NP_037038.1	amyloid-like protein 2 isoform 1 precursor
NM_022520.1	NP_071965.1	amyloid-like protein 2 isoform 2 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Aplp2	NP_037038.1
	APLP2	XP_001112057.2
Mus musculus (house mouse)	Aplp2	NP_001095925.1
Gallus gallus (chicken)	APLP2	NP_001006317.2
Pan troglodytes (chimpanzee)	APLP2	XP_001155401.1
Canis lupus familiaris (dog)	APLP2	XP_536530.2
Danio rerio (zebrafish)	aplp2	NP_001116484.1
Homo sapiens (human)	APLP2	NP_001633.1
Bos taurus (cattle)	APLP2	XP_002699281.1

Gene Ontology
Bibliography

Related articles in PubMed

A single amino acid difference between the intracellular domains of amyloid precursor protein and amyloid-like precursor protein 2 enables induction of synaptic depression and block of long-term potentiation.
Trillaud-Doppia E, Paradis-Isler N, Boehm J
Neurobiology of disease9194-104(2016 07)

Discrepancy in Expression of ?-Secretase and Amyloid-? Protein Precursor in Alzheimer-Related Genes in the Rat Medial Temporal Lobe Cortex Following Transient Global Brain Ischemia.
Pluta R, Kocki J, U?amek-Kozio? M, Petniak A, Gil-Kulik P, Januszewski S, Bogucki J, Jab?o?ski M, Brzozowska J, Furmaga-Jab?o?ska W, Bogucka-Kocka A, Czuczwar SJ
Journal of Alzheimer's disease : JAD51(4)1023-31(2016)

[Acute and chronic stress induced changes in gene transcriptions related to Alzheimer's disease].
S?ntha P, P?k?ski M, Fazekas O, Szucs S, Fodor EK, K?lm?n J Jr, K?lm?n S, Szab? G, Janka Z, K?lm?n J
Ideggyogyaszati szemle65(5-6)195-200(2012 May)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal of proteome research8(3)1304-14(2009 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study demonstrated that the downregulation of amyloid beta precursor in Rat Medial Temporal Lobe Cortex Following Transient Global Brain ischemia.
Title: Discrepancy in Expression of ?-Secretase and Amyloid-? Protein Precursor in Alzheimer-Related Genes in the Rat Medial Temporal Lobe Cortex Following Transient Global Brain Ischemia.

Findings demonstrate that both acute and chronic Immobilization Stress lead to gene transcriptional changes of beta-actin, APP and MAPK-1. These changes may lead to cognitive deterioration, and the development of Alzheimer disease.
Title: [Acute and chronic stress induced changes in gene transcriptions related to Alzheimer's disease].

The APLP2 gene has both a GAGA sequence and a CAGA box immediately upstream of a position corresponding to the APP gene CAGA box.
Title: Presence of a "CAGA box" in the APP gene unique to amyloid plaque-forming species and absent in all APLP-1/2 genes: implications in Alzheimer's disease.

The expression of APLP2 is up-regulated in the striatum following dopaminergic denervation
Title: Identification and comparative analysis of differentially expressed proteins in rat striatum following 6-hydroxydopamine lesions of the nigrostriatal pathway: up-regulation of amyloid precursor-like protein 2 expression.

The following Aplp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aplp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01274	NM_012906.1 Latest version!	Rattus norvegicus amyloid beta precursor like protein 2 (Aplp2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa12296	NM_022520.1 Latest version!	Rattus norvegicus amyloid beta precursor like protein 2 (Aplp2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Aplp2 ( NM_012906.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037038.1 Rattus norvegicus amyloid beta precursor like protein 2 (Aplp2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa01274
Clone ID Related Accession (Same CDS sequence)	NM_012906.1
Accession Version	NM_012906.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	amyloid-like protein 2 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FM044356.1, FM029818.1, CA340752.1, CB795867.1, FM030557.1, FM039816.1, CB700594.1, FM112636.1, FM104554.1, FM041002.1 and BP497339.1. Transcript Variant: This variant (1) is the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCCA CCGGGACCGC GGCCGCTGCG GCCACCGGCA AGCTTCTTGT CCTGCTGCTG 
CTCGGGCTCA CGGCACCCGC AGCGGCTCTG GCTGGCTATA TCGAGGCTCT CGCAGCCAAT 
GCTGGAACAG GCTTTGCGGT TGCTGAACCT CAGATTGCCA TGTTCTGTGG GAAGCTGAAC 
ATGCATGTGA ACATTCAGAC TGGGAAGTGG GAGCCTGACC CAACCGGCAC CAAGAGCTGC 
CTGGGAACAA AGGAGGAAGT TCTTCAGTAC TGCCAGGAGA TATATCCAGA GCTGCAGATC 
ACAAATGTGA TGGAAGCAAA CCAGCCAGTC AATATTGATA GTTGGTGCCG AAGGGACAAA 
AAACAGTGCA GGAGTCACAT TGTTATACCA TTCAAGTGTC TTGTGGGTGA ATTTGTAAGT 
GATGTTCTGC TAGTTCCAGA AAACTGCCAG TTTTTCCACC AAGAGCGGAT GGAGGTGTGT 
GAGAAGCATC AGCGGTGGCA CACAGTCGTC AAGGAGGCAT GTCTGACTGA GGGGATGACC 
TTATATAGCT ATGGCATGCT GCTGCCCTGT GGGGTAGACC AGTTCCATGG CACTGAGTAT 
GTGTGCTGCC CTCAGACAAA GGTTGTTGAC TCTGACTCCA CTATGTCCAA AGAAGAGGAG 
GAAGAAGAGG AAGAGGATGA AGAAGAAGAC TATGCTCTTG ATAAAAGTGA ATTTCCTACC 
GAAGCAGATC TGGAAGACTT CACAGAAGCA GCTGCAGATG AGGACGAGGA TGAGGAGGAG 
GAGGAGGAGG AGGAAGGGGA GGAAGTGGTA GAAGACCGTG ATTACTACTA TGACTCCTTT 
AAAGGAGATG ATTACAATGA GGAGAACCCA ACTGAACCCA GCAGCGATGG CACCATCTCA 
GACAAGGAGA TTGCTCACGA TGTTAAAGCT GTCTGCTCCC AGGAGGCCAT GACGGGGCCC 
TGCCGGGCCG TGATGCCTCG TTGGTACTTC GACCTCTCCA AGGGAAAGTG CGTACGCTTT 
ATATATGGCG GCTGCGGCGG CAACAGGAAC AATTTTGAGT CTGAGGATTA TTGTATGGCT 
GTGTGTAAAA CGATGATTCC CCCGACTCCC CTGCCAACCA ATGATGTGGA CGTGTATTTT 
GAGACGTCGG CTGATGACAA TGAGCACGCC CGCTTTCAGA AGGCTAAGGA GCAGTTGGAA 
ATTCGGCATC GCAGCCGAAT GGACAGGGTA AAAAAGGAGT GGGAAGAAGC AGAACTTCAA 
GCCAAGAACC TCCCCAAGGC AGAGCGACAG ACCCTCATTC AGCATTTCCA AGCTATGGTT 
AAAGCCTTAG AGAAGGAAGC AGCCAGTGAG AAGCAGCAGC TTGTGGAGAC CCATTTGGCC 
CGAGTAGAAG CAATGCTGAA TGACCGTCGG CGCATAGCTC TGGAAAACTA CCTGGCTGCC 
CTGCAGTCTG ACCCTCCTCG GCCACATCGC ATTCTTCAAG CTCTTCGTCG TTACGTCCGT 
GCTGAGAACA AAGATCGCCT GCACACCATT CGTCATTACC AACATGTGTT GGCTGTTGAC 
CCAGAAAAGG CTGCGCAGAT GAAATCCCAG GTGATGACAC ACCTCCATGT CATTGAAGAA 
AGAAGGAACC AAAGTCTCTC TCTGCTGTAC AAAGTGCCTT ATGTGGCTCA AGAGATTCAG 
GAGGAAATTG ATGAGCTCCT TCAGGAACAG CGAGCAGATA TGGACCAGTT TACCTCCTCC 
ATCTCAGAGA ACCCTGTGGA TGTCCGGGTG AGCTCTGAGG AAAGTGAGGA GATCCCGCCG 
TTCCACCCTT TCCATCCCTT CCCATCCTTG TCTGAGAACG AAGACACTCA GCCGGAGTTG 
TACCACCCAA TGAAAAAAGG ATCTGGAATG GCAGAGCAAG ATGGGGGCCT GATTGGTGCA 
GAAGAAAAGG TGATCAACAG TAAGAATAAA ATGGATGAAA ATATGGTCAT TGACGAGACT 
CTGGATGTTA AGGAAATGAT TTTCAATGCT GAGAGAGTTG GGGGTCTGGA GGAAGAGCCG 
GACTCTGTGG GGCCTCTACG GGAGGACTTC AGTTTGAGCA GCAGTGCCCT TATTGGCTTG 
CTGGTCATCG CGGTGGCCAT TGCTACAGTC ATCGTCATCA GCCTGGTGAT GCTGAGGAAG 
AGGCAATACG GCACCATCAG CCATGGCATT GTGGAGGTTG ATCCAATGCT CACCCCAGAA 
GAGCGTCACT TGAACAAGAT GCAGAACCAC GGCTATGAAA ACCCAACCTA CAAATACCTG 
GAGCAGATGC AGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037038.1
CDS	101..2398
Translation	MAATGTAAAAATGKLLVLLLLGLTAPAAALAGYIEALAANAGTGFAVAEPQIAMFCGKLNMHVNIQTGKWEPDPTGTKSCLGTKEEVLQYCQEIYPELQITNVMEANQPVNIDSWCRRDKKQCRSHIVIPFKCLVGEFVSDVLLVPENCQFFHQERMEVCEKHQRWHTVVKEACLTEGMTLYSYGMLLPCGVDQFHGTEYVCCPQTKVVDSDSTMSKEEEEEEEEDEEEDYALDKSEFPTEADLEDFTEAAADEDEDEEEEEEEEGEEVVEDRDYYYDSFKGDDYNEENPTEPSSDGTISDKEIAHDVKAVCSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVCKTMIPPTPLPTNDVDVYFETSADDNEHARFQKAKEQLEIRHRSRMDRVKKEWEEAELQAKNLPKAERQTLIQHFQAMVKALEKEAASEKQQLVETHLARVEAMLNDRRRIALENYLAALQSDPPRPHRILQALRRYVRAENKDRLHTIRHYQHVLAVDPEKAAQMKSQVMTHLHVIEERRNQSLSLLYKVPYVAQEIQEEIDELLQEQRADMDQFTSSISENPVDVRVSSEESEEIPPFHPFHPFPSLSENEDTQPELYHPMKKGSGMAEQDGGLIGAEEKVINSKNKMDENMVIDETLDVKEMIFNAERVGGLEEEPDSVGPLREDFSLSSSALIGLLVIAVAIATVIVISLVMLRKRQYGTISHGIVEVDPMLTPEERHLNKMQNHGYENPTYKYLEQMQI

Target ORF information:

RefSeq Version	NM_012906.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus amyloid beta precursor like protein 2 (Aplp2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012906.1

ORF Insert Sequence:

ATGGCGGCCA CCGGGACCGC GGCCGCTGCG GCCACCGGCA AGCTTCTTGT CCTGCTGCTG 
CTCGGGCTCA CGGCACCCGC AGCGGCTCTG GCTGGCTATA TCGAGGCTCT CGCAGCCAAT 
GCTGGAACAG GCTTTGCGGT TGCTGAACCT CAGATTGCCA TGTTCTGTGG GAAGCTGAAC 
ATGCATGTGA ACATTCAGAC TGGGAAGTGG GAGCCTGACC CAACCGGCAC CAAGAGCTGC 
CTGGGAACAA AGGAGGAAGT TCTTCAGTAC TGCCAGGAGA TATATCCAGA GCTGCAGATC 
ACAAATGTGA TGGAAGCAAA CCAGCCAGTC AATATTGATA GTTGGTGCCG AAGGGACAAA 
AAACAGTGCA GGAGTCACAT TGTTATACCA TTCAAGTGTC TTGTGGGTGA ATTTGTAAGT 
GATGTTCTGC TAGTTCCAGA AAACTGCCAG TTTTTCCACC AAGAGCGGAT GGAGGTGTGT 
GAGAAGCATC AGCGGTGGCA CACAGTCGTC AAGGAGGCAT GTCTGACTGA GGGGATGACC 
TTATATAGCT ATGGCATGCT GCTGCCCTGT GGGGTAGACC AGTTCCATGG CACTGAGTAT 
GTGTGCTGCC CTCAGACAAA GGTTGTTGAC TCTGACTCCA CTATGTCCAA AGAAGAGGAG 
GAAGAAGAGG AAGAGGATGA AGAAGAAGAC TATGCTCTTG ATAAAAGTGA ATTTCCTACC 
GAAGCAGATC TGGAAGACTT CACAGAAGCA GCTGCAGATG AGGACGAGGA TGAGGAGGAG 
GAGGAGGAGG AGGAAGGGGA GGAAGTGGTA GAAGACCGTG ATTACTACTA TGACTCCTTT 
AAAGGAGATG ATTACAATGA GGAGAACCCA ACTGAACCCA GCAGCGATGG CACCATCTCA 
GACAAGGAGA TTGCTCACGA TGTTAAAGCT GTCTGCTCCC AGGAGGCCAT GACGGGGCCC 
TGCCGGGCCG TGATGCCTCG TTGGTACTTC GACCTCTCCA AGGGAAAGTG CGTACGCTTT 
ATATATGGCG GCTGCGGCGG CAACAGGAAC AATTTTGAGT CTGAGGATTA TTGTATGGCT 
GTGTGTAAAA CGATGATTCC CCCGACTCCC CTGCCAACCA ATGATGTGGA CGTGTATTTT 
GAGACGTCGG CTGATGACAA TGAGCACGCC CGCTTTCAGA AGGCTAAGGA GCAGTTGGAA 
ATTCGGCATC GCAGCCGAAT GGACAGGGTA AAAAAGGAGT GGGAAGAAGC AGAACTTCAA 
GCCAAGAACC TCCCCAAGGC AGAGCGACAG ACCCTCATTC AGCATTTCCA AGCTATGGTT 
AAAGCCTTAG AGAAGGAAGC AGCCAGTGAG AAGCAGCAGC TTGTGGAGAC CCATTTGGCC 
CGAGTAGAAG CAATGCTGAA TGACCGTCGG CGCATAGCTC TGGAAAACTA CCTGGCTGCC 
CTGCAGTCTG ACCCTCCTCG GCCACATCGC ATTCTTCAAG CTCTTCGTCG TTACGTCCGT 
GCTGAGAACA AAGATCGCCT GCACACCATT CGTCATTACC AACATGTGTT GGCTGTTGAC 
CCAGAAAAGG CTGCGCAGAT GAAATCCCAG GTGATGACAC ACCTCCATGT CATTGAAGAA 
AGAAGGAACC AAAGTCTCTC TCTGCTGTAC AAAGTGCCTT ATGTGGCTCA AGAGATTCAG 
GAGGAAATTG ATGAGCTCCT TCAGGAACAG CGAGCAGATA TGGACCAGTT TACCTCCTCC 
ATCTCAGAGA ACCCTGTGGA TGTCCGGGTG AGCTCTGAGG AAAGTGAGGA GATCCCGCCG 
TTCCACCCTT TCCATCCCTT CCCATCCTTG TCTGAGAACG AAGACACTCA GCCGGAGTTG 
TACCACCCAA TGAAAAAAGG ATCTGGAATG GCAGAGCAAG ATGGGGGCCT GATTGGTGCA 
GAAGAAAAGG TGATCAACAG TAAGAATAAA ATGGATGAAA ATATGGTCAT TGACGAGACT 
CTGGATGTTA AGGAAATGAT TTTCAATGCT GAGAGAGTTG GGGGTCTGGA GGAAGAGCCG 
GACTCTGTGG GGCCTCTACG GGAGGACTTC AGTTTGAGCA GCAGTGCCCT TATTGGCTTG 
CTGGTCATCG CGGTGGCCAT TGCTACAGTC ATCGTCATCA GCCTGGTGAT GCTGAGGAAG 
AGGCAATACG GCACCATCAG CCATGGCATT GTGGAGGTTG ATCCAATGCT CACCCCAGAA 
GAGCGTCACT TGAACAAGAT GCAGAACCAC GGCTATGAAA ACCCAACCTA CAAATACCTG 
GAGCAGATGC AGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aplp2 ( NM_022520.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071965.1 Rattus norvegicus amyloid beta precursor like protein 2 (Aplp2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa12296
Clone ID Related Accession (Same CDS sequence)	NM_022520.1
Accession Version	NM_022520.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	amyloid-like protein 2 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FM044356.1, FM029818.1, CA340752.1, CB795867.1, FM030557.1, FM039816.1, CB700594.1, FM112636.1, FM104554.1, FM041002.1 and BP497339.1. On or before Nov 10, 2012 this sequence version replaced XM_001056214.3, XM_003754396.1, XM_343513.4. Transcript Variant: This variant (2) lacks an in-frame coding exon and encodes a shorter isoform (2), compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCCA CCGGGACCGC GGCCGCTGCG GCCACCGGCA AGCTTCTTGT CCTGCTGCTG 
CTCGGGCTCA CGGCACCCGC AGCGGCTCTG GCTGGCTATA TCGAGGCTCT CGCAGCCAAT 
GCTGGAACAG GCTTTGCGGT TGCTGAACCT CAGATTGCCA TGTTCTGTGG GAAGCTGAAC 
ATGCATGTGA ACATTCAGAC TGGGAAGTGG GAGCCTGACC CAACCGGCAC CAAGAGCTGC 
CTGGGAACAA AGGAGGAAGT TCTTCAGTAC TGCCAGGAGA TATATCCAGA GCTGCAGATC 
ACAAATGTGA TGGAAGCAAA CCAGCCAGTC AATATTGATA GTTGGTGCCG AAGGGACAAA 
AAACAGTGCA GGAGTCACAT TGTTATACCA TTCAAGTGTC TTGTGGGTGA ATTTGTAAGT 
GATGTTCTGC TAGTTCCAGA AAACTGCCAG TTTTTCCACC AAGAGCGGAT GGAGGTGTGT 
GAGAAGCATC AGCGGTGGCA CACAGTCGTC AAGGAGGCAT GTCTGACTGA GGGGATGACC 
TTATATAGCT ATGGCATGCT GCTGCCCTGT GGGGTAGACC AGTTCCATGG CACTGAGTAT 
GTGTGCTGCC CTCAGACAAA GGTTGTTGAC TCTGACTCCA CTATGTCCAA AGAAGAGGAG 
GAAGAAGAGG AAGAGGATGA AGAAGAAGAC TATGCTCTTG ATAAAAGTGA ATTTCCTACC 
GAAGCAGATC TGGAAGACTT CACAGAAGCA GCTGCAGATG AGGACGAGGA TGAGGAGGAG 
GAGGAGGAGG AGGAAGGGGA GGAAGTGGTA GAAGACCGTG ATTACTACTA TGACTCCTTT 
AAAGGAGATG ATTACAATGA GGAGAACCCA ACTGAACCCA GCAGCGATGG CACCATCTCA 
GACAAGGAGA TTGCTCACGA TGTTAAAGCT GTCTGCTCCC AGGAGGCCAT GACGGGGCCC 
TGCCGGGCCG TGATGCCTCG TTGGTACTTC GACCTCTCCA AGGGAAAGTG CGTACGCTTT 
ATATATGGCG GCTGCGGCGG CAACAGGAAC AATTTTGAGT CTGAGGATTA TTGTATGGCT 
GTGTGTAAAA CGATGATTCC CCCGACTCCC CTGCCAACCA ATGATGTGGA CGTGTATTTT 
GAGACGTCGG CTGATGACAA TGAGCACGCC CGCTTTCAGA AGGCTAAGGA GCAGTTGGAA 
ATTCGGCATC GCAGCCGAAT GGACAGGGTA AAAAAGGAGT GGGAAGAAGC AGAACTTCAA 
GCCAAGAACC TCCCCAAGGC AGAGCGACAG ACCCTCATTC AGCATTTCCA AGCTATGGTT 
AAAGCCTTAG AGAAGGAAGC AGCCAGTGAG AAGCAGCAGC TTGTGGAGAC CCATTTGGCC 
CGAGTAGAAG CAATGCTGAA TGACCGTCGG CGCATAGCTC TGGAAAACTA CCTGGCTGCC 
CTGCAGTCTG ACCCTCCTCG GCCACATCGC ATTCTTCAAG CTCTTCGTCG TTACGTCCGT 
GCTGAGAACA AAGATCGCCT GCACACCATT CGTCATTACC AACATGTGTT GGCTGTTGAC 
CCAGAAAAGG CTGCGCAGAT GAAATCCCAG GTGATGACAC ACCTCCATGT CATTGAAGAA 
AGAAGGAACC AAAGTCTCTC TCTGCTGTAC AAAGTGCCTT ATGTGGCTCA AGAGATTCAG 
GAGGAAATTG ATGAGCTCCT TCAGGAACAG CGAGCAGATA TGGACCAGTT TACCTCCTCC 
ATCTCAGAGA ACCCTGTGGA TGTCCGGGTG AGCTCTGAGG AAAGTGAGGA GATCCCGCCG 
TTCCACCCTT TCCATCCCTT CCCATCCTTG TCTGAGAACG AAGGATCTGG AATGGCAGAG 
CAAGATGGGG GCCTGATTGG TGCAGAAGAA AAGGTGATCA ACAGTAAGAA TAAAATGGAT 
GAAAATATGG TCATTGACGA GACTCTGGAT GTTAAGGAAA TGATTTTCAA TGCTGAGAGA 
GTTGGGGGTC TGGAGGAAGA GCCGGACTCT GTGGGGCCTC TACGGGAGGA CTTCAGTTTG 
AGCAGCAGTG CCCTTATTGG CTTGCTGGTC ATCGCGGTGG CCATTGCTAC AGTCATCGTC 
ATCAGCCTGG TGATGCTGAG GAAGAGGCAA TACGGCACCA TCAGCCATGG CATTGTGGAG 
GTTGATCCAA TGCTCACCCC AGAAGAGCGT CACTTGAACA AGATGCAGAA CCACGGCTAT 
GAAAACCCAA CCTACAAATA CCTGGAGCAG ATGCAGATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071965.1
CDS	101..2362
Translation	MAATGTAAAAATGKLLVLLLLGLTAPAAALAGYIEALAANAGTGFAVAEPQIAMFCGKLNMHVNIQTGKWEPDPTGTKSCLGTKEEVLQYCQEIYPELQITNVMEANQPVNIDSWCRRDKKQCRSHIVIPFKCLVGEFVSDVLLVPENCQFFHQERMEVCEKHQRWHTVVKEACLTEGMTLYSYGMLLPCGVDQFHGTEYVCCPQTKVVDSDSTMSKEEEEEEEEDEEEDYALDKSEFPTEADLEDFTEAAADEDEDEEEEEEEEGEEVVEDRDYYYDSFKGDDYNEENPTEPSSDGTISDKEIAHDVKAVCSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVCKTMIPPTPLPTNDVDVYFETSADDNEHARFQKAKEQLEIRHRSRMDRVKKEWEEAELQAKNLPKAERQTLIQHFQAMVKALEKEAASEKQQLVETHLARVEAMLNDRRRIALENYLAALQSDPPRPHRILQALRRYVRAENKDRLHTIRHYQHVLAVDPEKAAQMKSQVMTHLHVIEERRNQSLSLLYKVPYVAQEIQEEIDELLQEQRADMDQFTSSISENPVDVRVSSEESEEIPPFHPFHPFPSLSENEGSGMAEQDGGLIGAEEKVINSKNKMDENMVIDETLDVKEMIFNAERVGGLEEEPDSVGPLREDFSLSSSALIGLLVIAVAIATVIVISLVMLRKRQYGTISHGIVEVDPMLTPEERHLNKMQNHGYENPTYKYLEQMQI

Target ORF information:

RefSeq Version	NM_022520.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus amyloid beta precursor like protein 2 (Aplp2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022520.1

ORF Insert Sequence:

ATGGCGGCCA CCGGGACCGC GGCCGCTGCG GCCACCGGCA AGCTTCTTGT CCTGCTGCTG 
CTCGGGCTCA CGGCACCCGC AGCGGCTCTG GCTGGCTATA TCGAGGCTCT CGCAGCCAAT 
GCTGGAACAG GCTTTGCGGT TGCTGAACCT CAGATTGCCA TGTTCTGTGG GAAGCTGAAC 
ATGCATGTGA ACATTCAGAC TGGGAAGTGG GAGCCTGACC CAACCGGCAC CAAGAGCTGC 
CTGGGAACAA AGGAGGAAGT TCTTCAGTAC TGCCAGGAGA TATATCCAGA GCTGCAGATC 
ACAAATGTGA TGGAAGCAAA CCAGCCAGTC AATATTGATA GTTGGTGCCG AAGGGACAAA 
AAACAGTGCA GGAGTCACAT TGTTATACCA TTCAAGTGTC TTGTGGGTGA ATTTGTAAGT 
GATGTTCTGC TAGTTCCAGA AAACTGCCAG TTTTTCCACC AAGAGCGGAT GGAGGTGTGT 
GAGAAGCATC AGCGGTGGCA CACAGTCGTC AAGGAGGCAT GTCTGACTGA GGGGATGACC 
TTATATAGCT ATGGCATGCT GCTGCCCTGT GGGGTAGACC AGTTCCATGG CACTGAGTAT 
GTGTGCTGCC CTCAGACAAA GGTTGTTGAC TCTGACTCCA CTATGTCCAA AGAAGAGGAG 
GAAGAAGAGG AAGAGGATGA AGAAGAAGAC TATGCTCTTG ATAAAAGTGA ATTTCCTACC 
GAAGCAGATC TGGAAGACTT CACAGAAGCA GCTGCAGATG AGGACGAGGA TGAGGAGGAG 
GAGGAGGAGG AGGAAGGGGA GGAAGTGGTA GAAGACCGTG ATTACTACTA TGACTCCTTT 
AAAGGAGATG ATTACAATGA GGAGAACCCA ACTGAACCCA GCAGCGATGG CACCATCTCA 
GACAAGGAGA TTGCTCACGA TGTTAAAGCT GTCTGCTCCC AGGAGGCCAT GACGGGGCCC 
TGCCGGGCCG TGATGCCTCG TTGGTACTTC GACCTCTCCA AGGGAAAGTG CGTACGCTTT 
ATATATGGCG GCTGCGGCGG CAACAGGAAC AATTTTGAGT CTGAGGATTA TTGTATGGCT 
GTGTGTAAAA CGATGATTCC CCCGACTCCC CTGCCAACCA ATGATGTGGA CGTGTATTTT 
GAGACGTCGG CTGATGACAA TGAGCACGCC CGCTTTCAGA AGGCTAAGGA GCAGTTGGAA 
ATTCGGCATC GCAGCCGAAT GGACAGGGTA AAAAAGGAGT GGGAAGAAGC AGAACTTCAA 
GCCAAGAACC TCCCCAAGGC AGAGCGACAG ACCCTCATTC AGCATTTCCA AGCTATGGTT 
AAAGCCTTAG AGAAGGAAGC AGCCAGTGAG AAGCAGCAGC TTGTGGAGAC CCATTTGGCC 
CGAGTAGAAG CAATGCTGAA TGACCGTCGG CGCATAGCTC TGGAAAACTA CCTGGCTGCC 
CTGCAGTCTG ACCCTCCTCG GCCACATCGC ATTCTTCAAG CTCTTCGTCG TTACGTCCGT 
GCTGAGAACA AAGATCGCCT GCACACCATT CGTCATTACC AACATGTGTT GGCTGTTGAC 
CCAGAAAAGG CTGCGCAGAT GAAATCCCAG GTGATGACAC ACCTCCATGT CATTGAAGAA 
AGAAGGAACC AAAGTCTCTC TCTGCTGTAC AAAGTGCCTT ATGTGGCTCA AGAGATTCAG 
GAGGAAATTG ATGAGCTCCT TCAGGAACAG CGAGCAGATA TGGACCAGTT TACCTCCTCC 
ATCTCAGAGA ACCCTGTGGA TGTCCGGGTG AGCTCTGAGG AAAGTGAGGA GATCCCGCCG 
TTCCACCCTT TCCATCCCTT CCCATCCTTG TCTGAGAACG AAGGATCTGG AATGGCAGAG 
CAAGATGGGG GCCTGATTGG TGCAGAAGAA AAGGTGATCA ACAGTAAGAA TAAAATGGAT 
GAAAATATGG TCATTGACGA GACTCTGGAT GTTAAGGAAA TGATTTTCAA TGCTGAGAGA 
GTTGGGGGTC TGGAGGAAGA GCCGGACTCT GTGGGGCCTC TACGGGAGGA CTTCAGTTTG 
AGCAGCAGTG CCCTTATTGG CTTGCTGGTC ATCGCGGTGG CCATTGCTAC AGTCATCGTC 
ATCAGCCTGG TGATGCTGAG GAAGAGGCAA TACGGCACCA TCAGCCATGG CATTGTGGAG 
GTTGATCCAA TGCTCACCCC AGAAGAGCGT CACTTGAACA AGATGCAGAA CCACGGCTAT 
GAAAACCCAA CCTACAAATA CCTGGAGCAG ATGCAGATTT AA