gRNAs
The following Card9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Card9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa12933 | |
| Clone ID Related Accession (Same CDS sequence) | NM_022303.1 | |
| Accession Version | NM_022303.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | caspase recruitment domain-containing protein 9 | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF311288.1. On Jan 12, 2005 this sequence version replaced XM_346649.1. ##Evidence-Data-START## Transcript exon combination :: AF311288.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297, SAMEA2689594 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGTCAGACT ATGAAAATGA CGACGAGTGC TGGAGTGCCC TGGAGAGCTT CCGGGTGAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_071639.1 |
| CDS | 113..1723 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_022303.1 |
1 | ATGTCAGACT ATGAAAATGA CGACGAGTGC TGGAGTGCCC TGGAGAGCTT CCGGGTGAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa43566 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017592019.1 | |
| Accession Version | XM_017592019.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1359bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | caspase recruitment domain-containing protein 9 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGTCAGACT ATGAAAATGA CGACGAGTGC TGGAGTGCCC TGGAGAGCTT CCGGGTGAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017447508.1 |
| CDS | 230..1588 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017592019.1 |
1 | ATGTCAGACT ATGAAAATGA CGACGAGTGC TGGAGTGCCC TGGAGAGCTT CCGGGTGAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa43567 | |
| Clone ID Related Accession (Same CDS sequence) | XM_008761626.2 | |
| Accession Version | XM_008761626.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1296bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | caspase recruitment domain-containing protein 9 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761626.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGTCAGACT ATGAAAATGA CGACGAGTGC TGGAGTGCCC TGGAGAGCTT CCGGGTGAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_008759848.1 |
| CDS | 231..1526 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_008761626.2 |
1 | ATGTCAGACT ATGAAAATGA CGACGAGTGC TGGAGTGCCC TGGAGAGCTT CCGGGTGAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CARD9 gene silencing with siRNA protects rats against severe acute pancreatitis: CARD9-dependent NF-?B and P38MAPKs pathway. |
The adaptor protein CARD9 is required for innate immune responses to intracellular pathogens. |
Card9 controls a non-TLR signalling pathway for innate anti-fungal immunity. |
CARD9 is a novel caspase recruitment domain-containing protein that interacts with BCL10/CLAP and activates NF-kappa B. |
