Xylt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Xylt1
Entrez Gene ID	64133
Full Name	xylosyltransferase 1
Synonyms	XYLT-1,Xt1
General protein information	Preferred Names xylosyltransferase 1 Names xylosyltransferase 1 LOW QUALITY PROTEIN: xylosyltransferase 1 peptide O-xylosyltransferase 1 xylosyltransferase I
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q35

mRNA and Protein(s)

mRNA	Protein	Name
XM_006230151.2	XP_006230213.2	LOW QUALITY PROTEIN: xylosyltransferase 1 isoform X1
NM_022295.1	NP_071631.1	xylosyltransferase 1 precursor
XM_006223458.2	XP_006223520.1	xylosyltransferase 1 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	XYLT1	NP_001032366.1
Bos taurus (cattle)	XYLT1	XP_002698083.1
Mus musculus (house mouse)	Xylt1	NP_783576.2
Gallus gallus (chicken)	XYLT1	XP_414904.3
Drosophila melanogaster (fruit fly)	oxt	NP_647705.1
	LOC718187	XP_001110525.2
Xenopus tropicalis (tropical clawed frog)	xylt1	NP_001120412.1
Homo sapiens (human)	XYLT1	NP_071449.1
Canis lupus familiaris (dog)	XYLT1	NP_001008718.1
Rattus norvegicus (Norway rat)	Xylt1	XP_006230213.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005811	XP_556482.2
Danio rerio (zebrafish)	xylt1	NP_001165868.1

Gene Ontology
Bibliography

Related articles in PubMed

Xylosyltransferase-1 expression is refractory to inhibition by the inflammatory cytokines tumor necrosis factor ? and IL-1? in nucleus pulposus cells: novel regulation by AP-1, Sp1, and Sp3.
Ye W, Zhou J, Markova DZ, Tian Y, Li J, Anderson DG, Shapiro IM, Risbud MV
The American journal of pathology185(2)485-95(2015 Feb)

Forward genetics defines Xylt1 as a key, conserved regulator of early chondrocyte maturation and skeletal length.
Mis EK, Liem KF Jr, Kong Y, Schwartz NB, Domowicz M, Weatherbee SD
Developmental biology385(1)67-82(2014 Jan)

Modulation of xylosyltransferase I expression provides a mechanism regulating glycosaminoglycan chain synthesis during cartilage destruction and repair.
Venkatesan N, Barr? L, Magdalou J, Mainard D, Netter P, Fournel-Gigleux S, Ouzzine M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology23(3)813-22(2009 Mar)

Deoxyribozyme-mediated knockdown of xylosyltransferase-1 mRNA promotes axon growth in the adult rat spinal cord.
Hurtado A, Podinin H, Oudega M, Grimpe B
Brain : a journal of neurology131(Pt 10)2596-605(2008 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that XT-1 expression is refractory to the disease process and to inhibition by inflammatory cytokines and that signaling through AP-1, Sp1, and Sp3 is important in the maintenance of XT-1 levels in NP cells.
Title: Xylosyltransferase-1 expression is refractory to inhibition by the inflammatory cytokines tumor necrosis factor ? and IL-1? in nucleus pulposus cells: novel regulation by AP-1, Sp1, and Sp3.

Expression of xylosyltransferase I (XT-I) gene initiating proteoglycan-glycosaminoglycan chain synthesis was significantly reduced in antigen-induced arthritis rat cartilage and was associated with a decrease in proteoglycan synthesis.
Title: Modulation of xylosyltransferase I expression provides a mechanism regulating glycosaminoglycan chain synthesis during cartilage destruction and repair.

Xylosyltransferase and heparanase expression is upregulated in adriamycin nephropathy.
Title: [Gene expressions of xylosyltransferase and heparanase in renal tissue of rat with adriamycin nephropathy and relevance for proteinuria].

The following Xylt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Xylt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa14615	NM_022295.1 Latest version!	Rattus norvegicus xylosyltransferase 1 (Xylt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa14615	XM_006223458.2 Latest version!	Rattus norvegicus xylosyltransferase 1 (Xylt1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
ORa43559	XM_006230151.2 Latest version!	Rattus norvegicus xylosyltransferase 1 (Xylt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Xylt1 ( NM_022295.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071631.1 Rattus norvegicus xylosyltransferase 1 (Xylt1), mRNA.

Price & Availability↑

CloneID	ORa14615
Clone ID Related Accession (Same CDS sequence)	NM_022295.1 , XM_006223458.2
Accession Version	NM_022295.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Rattus norvegicus(Norway rat)
Product	xylosyltransferase 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAHX01008118.1, AAHX01008124.1, AAHX01008125.1, AAHX01008126.1 and AAHX01008128.1. On Oct 25, 2019 this sequence version replaced XM_006223458.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGTGGCGG CGCCGTGCGC TCGGAGGCTG GCGCGGCGCT CGCACTCGGC GCTGCTGGCG 
GCGCTCATGG TGCTGCTGCT GCAGACGCTG GTGGTGTGGA ATTTCAGCAG CCTGGACTCC 
GGGGCTGGAG AGCAGCGGCG CGCGGGGGCA GCGGCGGGAG CAGCGGAGCA GCAGCAGCAG 
CAGCCCGCGG CCCCGCGCCG GGAGCGCAGG GACCTGGCTG CCCATCTGCC CGCAGCCCGC 
GGAGGACCTG GAGGCCGAGC CGGCGGAGGA GGAGCACGGG GAGGCGGCCC CGGAGGAGCC 
CGCGCACAGC AGCCTGCCAG CCGCGGGGCA CTAGCCGCCC GGGCGAGGGA TCCACACCCA 
AGTCCACTGA TCACCCTAGA GACCCAGGAT GGCTACTTCT CTCACCGGCC CAAAGAGAAA 
GTTCGGACCG ACAGCAACAA TGAGAACTCA GTCCCCAAGG ACTTTGAGAA TGTGGACAAC 
AGCAACTTCG CACCCAGGAC CCAGAAGCAG AAGCACCAGC CAGAGTTGGC AAAGAAGCCC 
CTCAGCAGGC AGAAAGAGCG TTTGCAGAGA AAGCTGGGCG CCCAGGACAA AGGACAGGGG 
CAGTCAGTCC TAGGAAAAGG CCCCAAGGAG GTGCTGCCTC CTCGGGAAAA AGCCCCAGGC 
AACAGTAGCC AAGGGAAGGA TCTCTCAAGA CACAGCCATA GCAGGAAGAG TGGTGGCGGT 
GGGTCCCCGG AAACTAAGTC TGACCAGGTC CCCAAGTGTG ACATCTCTGG CAAGGAGGCC 
ATCTCAGCGC TGACCCGCGC TAAGTCCAAG CACTGTCGCC AGGAGATTGC AGAAACCTAC 
TGTCGCCACA AGCTGGGGCT GCTGATGCCA GAGAAGGTGG CTCGATTCTG TCCCCTGGAA 
GGCAAAGCCA ACAAGAATGT CCAGTGGGAT GAGGATGCTG TTGAGTACAT GCCCCCCAAC 
CCAGTCAGAA TCGCCTTTGT CCTGGTGGTC CATGGCCGTG CCTCTCGACA GCTGCAGCGC 
ATGTTTAAGG CCATCTACCA CAAAGACCAT TTCTACTATA TCCATGTGGA TAAGCGTTCC 
AATTACCTGC ATCGGCAAGT GCTCCAGTTC TCCAGGCAGT ACGACAACGT CCGAGTCACT 
TCCTGGAGGA TGGCCACCAT TTGGGGCGGA GCCAGCCTCC TGTCCACTTA CCTGCAGAGC 
ATGCGGGATC TACTGGAGAT GACTGACTGG CCCTGGGACT TCTTCATCAA CCTCAGTGCT 
GCTGACTACC CCATCAGGAC AAATGACCAG CTCGTAGCAT TTCTTTCCAG ATACCGAGAT 
ATGAATTTCC TGAAGTCACA TGGTCGGGAC AATGCAAGGT TCATCCGAAA GCAGGGCCTG 
GACCGTCTCT TCCTGGAGTG TGATACACAC ATGTGGCGCC TGGGGGACCG GCGAATCCCA 
GAGGGCATTG CTGTAGATGG CGGCTCTGAT TGGTTCCTGC TAAACCGGAA GTTTGTAGAG 
TATGTGGCAT TCTCCACAGA TGACCTGGTG ACCAAGATGA AGCAGTTCTA CTCTTATACC 
CTCCTCCCTG CTGAGTCCTT TTTCCACACG GTCCTGGAGA ACAGCCCCCA CTGTGACACC 
ATGGTGGATA ACAACCTGCG CATCACCAAT TGGAACCGCA AGCTGGGCTG CAAGTGCCAG 
TACAAGCATA TTGTGGACTG GTGTGGCTGC TCTCCCAATG ACTTCAAGCC TCAGGACTTC 
CATCGCTTCC AGCAGACGGC CCGGCCCACC TTCTTTGCCC GAAAGTTCGA AGCCATAGTG 
AACCAGGAAA TCATTGGGCA GTTGGACTCT TACCTGTATG GCAACTATCC TGCGGGCACC 
CCGGGCCTCC GCTCTTACTG GGAGAATGTC TATGACGAAC CAGATGGCAT CCAGAGCCTC 
AGCGATGTGG CACTCACCAT GTATCATTCC TTCATCCGCC TGGGTCTTCG AAGGGCTGAG 
TCATCGCTAC ACACGGATGG GGAGAACAGC TGCAGGTACT ATCCAATGGG CCACCCAGCG 
TCTGTCCACC TCTACTTCCT GGCTGACCGA TTCCAGGGCT TTCTGATCAA GCACCATGTG 
ACCAACCTTG CTGTGAGCAA ACTGGAGACA CTGGAGACAT GGATGATGCC AAAGAAAGTC 
TTCAAGGTCG CAAGTCCCCC CAGTGACTTT GGGAGGCTTC AGTTTTCTGA GGTTGGCACT 
GACTGGGATG CCAAGGAGAG ACTATTCCGG AACTTTGGTG GTCTTTTGGG GCCCATGGAT 
GAGCCGGTGG GAATGCAGAA ATGGGGGAAG GGACCCAATG TGACCGTGAC TGTTATTTGG 
GTGGATCCTG TCAATGTCAT CGCAGCCACC TATGATATCC TGATCGAGTC CACTGCGGAA 
TTCACACACT ACAAACCCCC TTTGAATCTG CCTCTGAGGC CTGGTGTCTG GACAGTGAAG 
ATTCTCCATC ACTGGGTACC AGTTGCAGAG ACCAAATTCC TTGTCGCACC TCTGACCTTC 
TCCAACAAGC AGCCCATCAA ACCAGAGGAG GCTTTGAAGC TGCACAATGG GCCACCTCGC 
AGTGCCTACA TGGAGCAGAG TTTCCAGAGC CTAAACCCAG TCCTCAGCCT GCACATCAAT 
CCTGCCCAAG TAGAGCAGGC CCGGAAGAAC GCAGCCTTCA CTGGGACAGC ACTAGAAGCC 
TGGCTGGACT CGCTGGTGGG TGGGACTTGG ACTGCCATGG ACGTCTGTGC CACAGGCCCC 
ACTGCCTGCC CAGTCATGCA GACCTGCAGC CAAACAGCCT GGAGTTCCTT CAGCCCTGAT 
CCCAAGTCAG AGCTGGGTGC AGTGAAGCCT GATGGTCGGC TCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071631.1
CDS	9..2876
Translation	MVAAPCARRLARRSHSALLAALMVLLLQTLVVWNFSSLDSGAGEQRRAGAAAGAAEQQQQQPAAPRRERRDLAAHLPAARGGPGGRAGGGGARGGGPGGARAQQPASRGALAARARDPHPSPLITLETQDGYFSHRPKEKVRTDSNNENSVPKDFENVDNSNFAPRTQKQKHQPELAKKPLSRQKERLQRKLGAQDKGQGQSVLGKGPKEVLPPREKAPGNSSQGKDLSRHSHSRKSGGGGSPETKSDQVPKCDISGKEAISALTRAKSKHCRQEIAETYCRHKLGLLMPEKVARFCPLEGKANKNVQWDEDAVEYMPPNPVRIAFVLVVHGRASRQLQRMFKAIYHKDHFYYIHVDKRSNYLHRQVLQFSRQYDNVRVTSWRMATIWGGASLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIRTNDQLVAFLSRYRDMNFLKSHGRDNARFIRKQGLDRLFLECDTHMWRLGDRRIPEGIAVDGGSDWFLLNRKFVEYVAFSTDDLVTKMKQFYSYTLLPAESFFHTVLENSPHCDTMVDNNLRITNWNRKLGCKCQYKHIVDWCGCSPNDFKPQDFHRFQQTARPTFFARKFEAIVNQEIIGQLDSYLYGNYPAGTPGLRSYWENVYDEPDGIQSLSDVALTMYHSFIRLGLRRAESSLHTDGENSCRYYPMGHPASVHLYFLADRFQGFLIKHHVTNLAVSKLETLETWMMPKKVFKVASPPSDFGRLQFSEVGTDWDAKERLFRNFGGLLGPMDEPVGMQKWGKGPNVTVTVIWVDPVNVIAATYDILIESTAEFTHYKPPLNLPLRPGVWTVKILHHWVPVAETKFLVAPLTFSNKQPIKPEEALKLHNGPPRSAYMEQSFQSLNPVLSLHINPAQVEQARKNAAFTGTALEAWLDSLVGGTWTAMDVCATGPTACPVMQTCSQTAWSSFSPDPKSELGAVKPDGRLR

Target ORF information:

RefSeq Version	NM_022295.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus xylosyltransferase 1 (Xylt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022295.1

ORF Insert Sequence:

ATGGTGGCGG CGCCGTGCGC TCGGAGGCTG GCGCGGCGCT CGCACTCGGC GCTGCTGGCG 
GCGCTCATGG TGCTGCTGCT GCAGACGCTG GTGGTGTGGA ATTTCAGCAG CCTGGACTCC 
GGGGCTGGAG AGCAGCGGCG CGCGGGGGCA GCGGCGGGAG CAGCGGAGCA GCAGCAGCAG 
CAGCCCGCGG CCCCGCGCCG GGAGCGCAGG GACCTGGCTG CCCATCTGCC CGCAGCCCGC 
GGAGGACCTG GAGGCCGAGC CGGCGGAGGA GGAGCACGGG GAGGCGGCCC CGGAGGAGCC 
CGCGCACAGC AGCCTGCCAG CCGCGGGGCA CTAGCCGCCC GGGCGAGGGA TCCACACCCA 
AGTCCACTGA TCACCCTAGA GACCCAGGAT GGCTACTTCT CTCACCGGCC CAAAGAGAAA 
GTTCGGACCG ACAGCAACAA TGAGAACTCA GTCCCCAAGG ACTTTGAGAA TGTGGACAAC 
AGCAACTTCG CACCCAGGAC CCAGAAGCAG AAGCACCAGC CAGAGTTGGC AAAGAAGCCC 
CTCAGCAGGC AGAAAGAGCG TTTGCAGAGA AAGCTGGGCG CCCAGGACAA AGGACAGGGG 
CAGTCAGTCC TAGGAAAAGG CCCCAAGGAG GTGCTGCCTC CTCGGGAAAA AGCCCCAGGC 
AACAGTAGCC AAGGGAAGGA TCTCTCAAGA CACAGCCATA GCAGGAAGAG TGGTGGCGGT 
GGGTCCCCGG AAACTAAGTC TGACCAGGTC CCCAAGTGTG ACATCTCTGG CAAGGAGGCC 
ATCTCAGCGC TGACCCGCGC TAAGTCCAAG CACTGTCGCC AGGAGATTGC AGAAACCTAC 
TGTCGCCACA AGCTGGGGCT GCTGATGCCA GAGAAGGTGG CTCGATTCTG TCCCCTGGAA 
GGCAAAGCCA ACAAGAATGT CCAGTGGGAT GAGGATGCTG TTGAGTACAT GCCCCCCAAC 
CCAGTCAGAA TCGCCTTTGT CCTGGTGGTC CATGGCCGTG CCTCTCGACA GCTGCAGCGC 
ATGTTTAAGG CCATCTACCA CAAAGACCAT TTCTACTATA TCCATGTGGA TAAGCGTTCC 
AATTACCTGC ATCGGCAAGT GCTCCAGTTC TCCAGGCAGT ACGACAACGT CCGAGTCACT 
TCCTGGAGGA TGGCCACCAT TTGGGGCGGA GCCAGCCTCC TGTCCACTTA CCTGCAGAGC 
ATGCGGGATC TACTGGAGAT GACTGACTGG CCCTGGGACT TCTTCATCAA CCTCAGTGCT 
GCTGACTACC CCATCAGGAC AAATGACCAG CTCGTAGCAT TTCTTTCCAG ATACCGAGAT 
ATGAATTTCC TGAAGTCACA TGGTCGGGAC AATGCAAGGT TCATCCGAAA GCAGGGCCTG 
GACCGTCTCT TCCTGGAGTG TGATACACAC ATGTGGCGCC TGGGGGACCG GCGAATCCCA 
GAGGGCATTG CTGTAGATGG CGGCTCTGAT TGGTTCCTGC TAAACCGGAA GTTTGTAGAG 
TATGTGGCAT TCTCCACAGA TGACCTGGTG ACCAAGATGA AGCAGTTCTA CTCTTATACC 
CTCCTCCCTG CTGAGTCCTT TTTCCACACG GTCCTGGAGA ACAGCCCCCA CTGTGACACC 
ATGGTGGATA ACAACCTGCG CATCACCAAT TGGAACCGCA AGCTGGGCTG CAAGTGCCAG 
TACAAGCATA TTGTGGACTG GTGTGGCTGC TCTCCCAATG ACTTCAAGCC TCAGGACTTC 
CATCGCTTCC AGCAGACGGC CCGGCCCACC TTCTTTGCCC GAAAGTTCGA AGCCATAGTG 
AACCAGGAAA TCATTGGGCA GTTGGACTCT TACCTGTATG GCAACTATCC TGCGGGCACC 
CCGGGCCTCC GCTCTTACTG GGAGAATGTC TATGACGAAC CAGATGGCAT CCAGAGCCTC 
AGCGATGTGG CACTCACCAT GTATCATTCC TTCATCCGCC TGGGTCTTCG AAGGGCTGAG 
TCATCGCTAC ACACGGATGG GGAGAACAGC TGCAGGTACT ATCCAATGGG CCACCCAGCG 
TCTGTCCACC TCTACTTCCT GGCTGACCGA TTCCAGGGCT TTCTGATCAA GCACCATGTG 
ACCAACCTTG CTGTGAGCAA ACTGGAGACA CTGGAGACAT GGATGATGCC AAAGAAAGTC 
TTCAAGGTCG CAAGTCCCCC CAGTGACTTT GGGAGGCTTC AGTTTTCTGA GGTTGGCACT 
GACTGGGATG CCAAGGAGAG ACTATTCCGG AACTTTGGTG GTCTTTTGGG GCCCATGGAT 
GAGCCGGTGG GAATGCAGAA ATGGGGGAAG GGACCCAATG TGACCGTGAC TGTTATTTGG 
GTGGATCCTG TCAATGTCAT CGCAGCCACC TATGATATCC TGATCGAGTC CACTGCGGAA 
TTCACACACT ACAAACCCCC TTTGAATCTG CCTCTGAGGC CTGGTGTCTG GACAGTGAAG 
ATTCTCCATC ACTGGGTACC AGTTGCAGAG ACCAAATTCC TTGTCGCACC TCTGACCTTC 
TCCAACAAGC AGCCCATCAA ACCAGAGGAG GCTTTGAAGC TGCACAATGG GCCACCTCGC 
AGTGCCTACA TGGAGCAGAG TTTCCAGAGC CTAAACCCAG TCCTCAGCCT GCACATCAAT 
CCTGCCCAAG TAGAGCAGGC CCGGAAGAAC GCAGCCTTCA CTGGGACAGC ACTAGAAGCC 
TGGCTGGACT CGCTGGTGGG TGGGACTTGG ACTGCCATGG ACGTCTGTGC CACAGGCCCC 
ACTGCCTGCC CAGTCATGCA GACCTGCAGC CAAACAGCCT GGAGTTCCTT CAGCCCTGAT 
CCCAAGTCAG AGCTGGGTGC AGTGAAGCCT GATGGTCGGC TCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Xylt1 ( XM_006223458.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006223520.1 Rattus norvegicus xylosyltransferase 1 (Xylt1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa14615
Clone ID Related Accession (Same CDS sequence)	NM_022295.1 , XM_006223458.2
Accession Version	XM_006223458.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	xylosyltransferase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000069.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006223458.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCGG CGCCGTGCGC TCGGAGGCTG GCGCGGCGCT CGCACTCGGC GCTGCTGGCG 
GCGCTCATGG TGCTGCTGCT GCAGACGCTG GTGGTGTGGA ATTTCAGCAG CCTGGACTCC 
GGGGCTGGAG AGCAGCGGCG CGCGGGGGCA GCGGCGGGAG CAGCGGAGCA GCAGCAGCAG 
CAGCCCGCGG CCCCGCGCCG GGAGCGCAGG GACCTGGCTG CCCATCTGCC CGCAGCCCGC 
GGAGGACCTG GAGGCCGAGC CGGCGGAGGA GGAGCACGGG GAGGCGGCCC CGGAGGAGCC 
CGCGCACAGC AGCCTGCCAG CCGCGGGGCA CTAGCCGCCC GGGCGAGGGA TCCACACCCA 
AGTCCACTGA TCACCCTAGA GACCCAGGAT GGCTACTTCT CTCACCGGCC CAAAGAGAAA 
GTTCGGACCG ACAGCAACAA TGAGAACTCA GTCCCCAAGG ACTTTGAGAA TGTGGACAAC 
AGCAACTTCG CACCCAGGAC CCAGAAGCAG AAGCACCAGC CAGAGTTGGC AAAGAAGCCC 
CTCAGCAGGC AGAAAGAGCG TTTGCAGAGA AAGCTGGGCG CCCAGGACAA AGGACAGGGG 
CAGTCAGTCC TAGGAAAAGG CCCCAAGGAG GTGCTGCCTC CTCGGGAAAA AGCCCCAGGC 
AACAGTAGCC AAGGGAAGGA TCTCTCAAGA CACAGCCATA GCAGGAAGAG TGGTGGCGGT 
GGGTCCCCGG AAACTAAGTC TGACCAGGTC CCCAAGTGTG ACATCTCTGG CAAGGAGGCC 
ATCTCAGCGC TGACCCGCGC TAAGTCCAAG CACTGTCGCC AGGAGATTGC AGAAACCTAC 
TGTCGCCACA AGCTGGGGCT GCTGATGCCA GAGAAGGTGG CTCGATTCTG TCCCCTGGAA 
GGCAAAGCCA ACAAGAATGT CCAGTGGGAT GAGGATGCTG TTGAGTACAT GCCCCCCAAC 
CCAGTCAGAA TCGCCTTTGT CCTGGTGGTC CATGGCCGTG CCTCTCGACA GCTGCAGCGC 
ATGTTTAAGG CCATCTACCA CAAAGACCAT TTCTACTATA TCCATGTGGA TAAGCGTTCC 
AATTACCTGC ATCGGCAAGT GCTCCAGTTC TCCAGGCAGT ACGACAACGT CCGAGTCACT 
TCCTGGAGGA TGGCCACCAT TTGGGGCGGA GCCAGCCTCC TGTCCACTTA CCTGCAGAGC 
ATGCGGGATC TACTGGAGAT GACTGACTGG CCCTGGGACT TCTTCATCAA CCTCAGTGCT 
GCTGACTACC CCATCAGGAC AAATGACCAG CTCGTAGCAT TTCTTTCCAG ATACCGAGAT 
ATGAATTTCC TGAAGTCACA TGGTCGGGAC AATGCAAGGT TCATCCGAAA GCAGGGCCTG 
GACCGTCTCT TCCTGGAGTG TGATACACAC ATGTGGCGCC TGGGGGACCG GCGAATCCCA 
GAGGGCATTG CTGTAGATGG CGGCTCTGAT TGGTTCCTGC TAAACCGGAA GTTTGTAGAG 
TATGTGGCAT TCTCCACAGA TGACCTGGTG ACCAAGATGA AGCAGTTCTA CTCTTATACC 
CTCCTCCCTG CTGAGTCCTT TTTCCACACG GTCCTGGAGA ACAGCCCCCA CTGTGACACC 
ATGGTGGATA ACAACCTGCG CATCACCAAT TGGAACCGCA AGCTGGGCTG CAAGTGCCAG 
TACAAGCATA TTGTGGACTG GTGTGGCTGC TCTCCCAATG ACTTCAAGCC TCAGGACTTC 
CATCGCTTCC AGCAGACGGC CCGGCCCACC TTCTTTGCCC GAAAGTTCGA AGCCATAGTG 
AACCAGGAAA TCATTGGGCA GTTGGACTCT TACCTGTATG GCAACTATCC TGCGGGCACC 
CCGGGCCTCC GCTCTTACTG GGAGAATGTC TATGACGAAC CAGATGGCAT CCAGAGCCTC 
AGCGATGTGG CACTCACCAT GTATCATTCC TTCATCCGCC TGGGTCTTCG AAGGGCTGAG 
TCATCGCTAC ACACGGATGG GGAGAACAGC TGCAGGTACT ATCCAATGGG CCACCCAGCG 
TCTGTCCACC TCTACTTCCT GGCTGACCGA TTCCAGGGCT TTCTGATCAA GCACCATGTG 
ACCAACCTTG CTGTGAGCAA ACTGGAGACA CTGGAGACAT GGATGATGCC AAAGAAAGTC 
TTCAAGGTCG CAAGTCCCCC CAGTGACTTT GGGAGGCTTC AGTTTTCTGA GGTTGGCACT 
GACTGGGATG CCAAGGAGAG ACTATTCCGG AACTTTGGTG GTCTTTTGGG GCCCATGGAT 
GAGCCGGTGG GAATGCAGAA ATGGGGGAAG GGACCCAATG TGACCGTGAC TGTTATTTGG 
GTGGATCCTG TCAATGTCAT CGCAGCCACC TATGATATCC TGATCGAGTC CACTGCGGAA 
TTCACACACT ACAAACCCCC TTTGAATCTG CCTCTGAGGC CTGGTGTCTG GACAGTGAAG 
ATTCTCCATC ACTGGGTACC AGTTGCAGAG ACCAAATTCC TTGTCGCACC TCTGACCTTC 
TCCAACAAGC AGCCCATCAA ACCAGAGGAG GCTTTGAAGC TGCACAATGG GCCACCTCGC 
AGTGCCTACA TGGAGCAGAG TTTCCAGAGC CTAAACCCAG TCCTCAGCCT GCACATCAAT 
CCTGCCCAAG TAGAGCAGGC CCGGAAGAAC GCAGCCTTCA CTGGGACAGC ACTAGAAGCC 
TGGCTGGACT CGCTGGTGGG TGGGACTTGG ACTGCCATGG ACGTCTGTGC CACAGGCCCC 
ACTGCCTGCC CAGTCATGCA GACCTGCAGC CAAACAGCCT GGAGTTCCTT CAGCCCTGAT 
CCCAAGTCAG AGCTGGGTGC AGTGAAGCCT GATGGTCGGC TCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006223520.1
CDS	9..2876
Translation	MVAAPCARRLARRSHSALLAALMVLLLQTLVVWNFSSLDSGAGEQRRAGAAAGAAEQQQQQPAAPRRERRDLAAHLPAARGGPGGRAGGGGARGGGPGGARAQQPASRGALAARARDPHPSPLITLETQDGYFSHRPKEKVRTDSNNENSVPKDFENVDNSNFAPRTQKQKHQPELAKKPLSRQKERLQRKLGAQDKGQGQSVLGKGPKEVLPPREKAPGNSSQGKDLSRHSHSRKSGGGGSPETKSDQVPKCDISGKEAISALTRAKSKHCRQEIAETYCRHKLGLLMPEKVARFCPLEGKANKNVQWDEDAVEYMPPNPVRIAFVLVVHGRASRQLQRMFKAIYHKDHFYYIHVDKRSNYLHRQVLQFSRQYDNVRVTSWRMATIWGGASLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIRTNDQLVAFLSRYRDMNFLKSHGRDNARFIRKQGLDRLFLECDTHMWRLGDRRIPEGIAVDGGSDWFLLNRKFVEYVAFSTDDLVTKMKQFYSYTLLPAESFFHTVLENSPHCDTMVDNNLRITNWNRKLGCKCQYKHIVDWCGCSPNDFKPQDFHRFQQTARPTFFARKFEAIVNQEIIGQLDSYLYGNYPAGTPGLRSYWENVYDEPDGIQSLSDVALTMYHSFIRLGLRRAESSLHTDGENSCRYYPMGHPASVHLYFLADRFQGFLIKHHVTNLAVSKLETLETWMMPKKVFKVASPPSDFGRLQFSEVGTDWDAKERLFRNFGGLLGPMDEPVGMQKWGKGPNVTVTVIWVDPVNVIAATYDILIESTAEFTHYKPPLNLPLRPGVWTVKILHHWVPVAETKFLVAPLTFSNKQPIKPEEALKLHNGPPRSAYMEQSFQSLNPVLSLHINPAQVEQARKNAAFTGTALEAWLDSLVGGTWTAMDVCATGPTACPVMQTCSQTAWSSFSPDPKSELGAVKPDGRLR

Target ORF information:

RefSeq Version	XM_006223458.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus xylosyltransferase 1 (Xylt1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006223458.2

ORF Insert Sequence:

ATGGTGGCGG CGCCGTGCGC TCGGAGGCTG GCGCGGCGCT CGCACTCGGC GCTGCTGGCG 
GCGCTCATGG TGCTGCTGCT GCAGACGCTG GTGGTGTGGA ATTTCAGCAG CCTGGACTCC 
GGGGCTGGAG AGCAGCGGCG CGCGGGGGCA GCGGCGGGAG CAGCGGAGCA GCAGCAGCAG 
CAGCCCGCGG CCCCGCGCCG GGAGCGCAGG GACCTGGCTG CCCATCTGCC CGCAGCCCGC 
GGAGGACCTG GAGGCCGAGC CGGCGGAGGA GGAGCACGGG GAGGCGGCCC CGGAGGAGCC 
CGCGCACAGC AGCCTGCCAG CCGCGGGGCA CTAGCCGCCC GGGCGAGGGA TCCACACCCA 
AGTCCACTGA TCACCCTAGA GACCCAGGAT GGCTACTTCT CTCACCGGCC CAAAGAGAAA 
GTTCGGACCG ACAGCAACAA TGAGAACTCA GTCCCCAAGG ACTTTGAGAA TGTGGACAAC 
AGCAACTTCG CACCCAGGAC CCAGAAGCAG AAGCACCAGC CAGAGTTGGC AAAGAAGCCC 
CTCAGCAGGC AGAAAGAGCG TTTGCAGAGA AAGCTGGGCG CCCAGGACAA AGGACAGGGG 
CAGTCAGTCC TAGGAAAAGG CCCCAAGGAG GTGCTGCCTC CTCGGGAAAA AGCCCCAGGC 
AACAGTAGCC AAGGGAAGGA TCTCTCAAGA CACAGCCATA GCAGGAAGAG TGGTGGCGGT 
GGGTCCCCGG AAACTAAGTC TGACCAGGTC CCCAAGTGTG ACATCTCTGG CAAGGAGGCC 
ATCTCAGCGC TGACCCGCGC TAAGTCCAAG CACTGTCGCC AGGAGATTGC AGAAACCTAC 
TGTCGCCACA AGCTGGGGCT GCTGATGCCA GAGAAGGTGG CTCGATTCTG TCCCCTGGAA 
GGCAAAGCCA ACAAGAATGT CCAGTGGGAT GAGGATGCTG TTGAGTACAT GCCCCCCAAC 
CCAGTCAGAA TCGCCTTTGT CCTGGTGGTC CATGGCCGTG CCTCTCGACA GCTGCAGCGC 
ATGTTTAAGG CCATCTACCA CAAAGACCAT TTCTACTATA TCCATGTGGA TAAGCGTTCC 
AATTACCTGC ATCGGCAAGT GCTCCAGTTC TCCAGGCAGT ACGACAACGT CCGAGTCACT 
TCCTGGAGGA TGGCCACCAT TTGGGGCGGA GCCAGCCTCC TGTCCACTTA CCTGCAGAGC 
ATGCGGGATC TACTGGAGAT GACTGACTGG CCCTGGGACT TCTTCATCAA CCTCAGTGCT 
GCTGACTACC CCATCAGGAC AAATGACCAG CTCGTAGCAT TTCTTTCCAG ATACCGAGAT 
ATGAATTTCC TGAAGTCACA TGGTCGGGAC AATGCAAGGT TCATCCGAAA GCAGGGCCTG 
GACCGTCTCT TCCTGGAGTG TGATACACAC ATGTGGCGCC TGGGGGACCG GCGAATCCCA 
GAGGGCATTG CTGTAGATGG CGGCTCTGAT TGGTTCCTGC TAAACCGGAA GTTTGTAGAG 
TATGTGGCAT TCTCCACAGA TGACCTGGTG ACCAAGATGA AGCAGTTCTA CTCTTATACC 
CTCCTCCCTG CTGAGTCCTT TTTCCACACG GTCCTGGAGA ACAGCCCCCA CTGTGACACC 
ATGGTGGATA ACAACCTGCG CATCACCAAT TGGAACCGCA AGCTGGGCTG CAAGTGCCAG 
TACAAGCATA TTGTGGACTG GTGTGGCTGC TCTCCCAATG ACTTCAAGCC TCAGGACTTC 
CATCGCTTCC AGCAGACGGC CCGGCCCACC TTCTTTGCCC GAAAGTTCGA AGCCATAGTG 
AACCAGGAAA TCATTGGGCA GTTGGACTCT TACCTGTATG GCAACTATCC TGCGGGCACC 
CCGGGCCTCC GCTCTTACTG GGAGAATGTC TATGACGAAC CAGATGGCAT CCAGAGCCTC 
AGCGATGTGG CACTCACCAT GTATCATTCC TTCATCCGCC TGGGTCTTCG AAGGGCTGAG 
TCATCGCTAC ACACGGATGG GGAGAACAGC TGCAGGTACT ATCCAATGGG CCACCCAGCG 
TCTGTCCACC TCTACTTCCT GGCTGACCGA TTCCAGGGCT TTCTGATCAA GCACCATGTG 
ACCAACCTTG CTGTGAGCAA ACTGGAGACA CTGGAGACAT GGATGATGCC AAAGAAAGTC 
TTCAAGGTCG CAAGTCCCCC CAGTGACTTT GGGAGGCTTC AGTTTTCTGA GGTTGGCACT 
GACTGGGATG CCAAGGAGAG ACTATTCCGG AACTTTGGTG GTCTTTTGGG GCCCATGGAT 
GAGCCGGTGG GAATGCAGAA ATGGGGGAAG GGACCCAATG TGACCGTGAC TGTTATTTGG 
GTGGATCCTG TCAATGTCAT CGCAGCCACC TATGATATCC TGATCGAGTC CACTGCGGAA 
TTCACACACT ACAAACCCCC TTTGAATCTG CCTCTGAGGC CTGGTGTCTG GACAGTGAAG 
ATTCTCCATC ACTGGGTACC AGTTGCAGAG ACCAAATTCC TTGTCGCACC TCTGACCTTC 
TCCAACAAGC AGCCCATCAA ACCAGAGGAG GCTTTGAAGC TGCACAATGG GCCACCTCGC 
AGTGCCTACA TGGAGCAGAG TTTCCAGAGC CTAAACCCAG TCCTCAGCCT GCACATCAAT 
CCTGCCCAAG TAGAGCAGGC CCGGAAGAAC GCAGCCTTCA CTGGGACAGC ACTAGAAGCC 
TGGCTGGACT CGCTGGTGGG TGGGACTTGG ACTGCCATGG ACGTCTGTGC CACAGGCCCC 
ACTGCCTGCC CAGTCATGCA GACCTGCAGC CAAACAGCCT GGAGTTCCTT CAGCCCTGAT 
CCCAAGTCAG AGCTGGGTGC AGTGAAGCCT GATGGTCGGC TCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Xylt1 ( XM_006230151.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006230213.2 Rattus norvegicus xylosyltransferase 1 (Xylt1), transcript variant X1, mRNA.

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CloneID	ORa43559
Clone ID Related Accession (Same CDS sequence)	XM_006230151.2
Accession Version	XM_006230151.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	LOW QUALITY PROTEIN: xylosyltransferase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006230151.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases frameshifts :: corrected 3 indels ##RefSeq-Attributes-END##

ATGCAGGAGA TTCAGAGAGT GGACACAGAG GAAACAGATA TGGACTACAG TGAAGCAGAG 
AAAGACACGG GGTGTCCCGG CACCCGCGCC CCCGGGCGCC GCGGAAGATG GGCGGCGCCC 
TGTGGCGAGG CTGGCGCCGG CGGCTCGCAC TCGGCGTGCG TGGCGGGCGC TATGGTGGCG 
CTGCTGCAGA CGCTGTGCGT GTGGAATTTC AGCAGCGTGG ACTCGGCCTG GAGGAGAGCG 
GCGCGCGGGG CGCGAGCGGC CGGGAGCAGC ACAGCGCAGC AGCAGCCCGC GGCCCCGCGC 
CGGGAGCGCA GGGACCTGGC TGCCCATCTG CCCGCAGCCC GCGGAGGACC TGGAGGCCGA 
GCCGGCGGAG GAGGAGCACG GGGAGGCGGC CCCGGAGGAG CCCGCGCACA GCAGCCTGCC 
AGCCGCGGGG CACTAGCCGC CCGGGCGAGG GATCCACACC CAAGTCCACT GATCACCCTA 
GAGACCCAGG ATGGCTACTT CTCTCACCGG CCCAAAGAGA AAGTTCGGAC CGACAGCAAC 
AATGAGAACT CAGTCCCCAA GGACTTTGAG AATGTGGACA ACAGCAACTT CGCACCCAGG 
ACCCAGAAGC AGAAGCACCA GCCAGAGTTG GCAAAGAAGC CCCTCAGCAG GCAGAAAGAG 
CGTTTGCAGA GAAAGCTGGG CGCCCAGGAC AAAGGACAGG GGCAGTCAGT CCTAGGAAAA 
GGCCCCAAGG AGGTGCTGCC TCCTCGGGAA AAAGCCCCAG GCAACAGTAG CCAAGGGAAG 
GATCTCTCAA GACACAGCCA TAGCAGGAAG AGTGGTGGCG GTGGGTCCCC GGAAACTAAG 
TCTGACCAGG TCCCCAAGTG TGACATCTCT GGCAAGGAGG CCATCTCAGC GCTGACCCGC 
GCTAAGTCCA AGCACTGTCG CCAGGAGATT GCAGAAACCT ACTGTCGCCA CAAGCTGGGG 
CTGCTGATGC CAGAGAAGGT GGCTCGATTC TGTCCCCTGG AAGGCAAAGC CAACAAGAAT 
GTCCAGTGGG ATGAGGATGC TGTTGAGTAC ATGCCCCCCA ACCCAGTCAG AATCGCCTTT 
GTCCTGGTGG TCCATGGCCG TGCCTCTCGA CAGCTGCAGC GCATGTTTAA GGCCATCTAC 
CACAAAGACC ATTTCTACTA TATCCATGTG GATAAGCGTT CCAATTACCT GCATCGGCAA 
GTGCTCCAGT TCTCCAGGCA GTACGACAAC GTCCGAGTCA CTTCCTGGAG GATGGCCACC 
ATTTGGGGCG GAGCCAGCCT CCTGTCCACT TACCTGCAGA GCATGCGGGA TCTACTGGAG 
ATGACTGACT GGCCCTGGGA CTTCTTCATC AACCTCAGTG CTGCTGACTA CCCCATCAGG 
ACAAATGACC AGCTCGTAGC ATTTCTTTCC AGATACCGAG ATATGAATTT CCTGAAGTCA 
CATGGTCGGG ACAATGCAAG GTTCATCCGA AAGCAGGGCC TGGACCGTCT CTTCCTGGAG 
TGTGATACAC ACATGTGGCG CCTGGGGGAC CGGCGAATCC CAGAGGGCAT TGCTGTAGAT 
GGCGGCTCTG ATTGGTTCCT GCTAAACCGG AAGTTTGTAG AGTATGTGGC ATTCTCCACA 
GATGACCTGG TGACCAAGAT GAAGCAGTTC TACTCTTATA CCCTCCTCCC TGCTGAGTCC 
TTTTTCCACA CGGTCCTGGA GAACAGCCCC CACTGTGACA CCATGGTGGA TAACAACCTG 
CGCATCACCA ATTGGAACCG CAAGCTGGGC TGCAAGTGCC AGTACAAGCA TATTGTGGAC 
TGGTGTGGCT GCTCTCCCAA TGACTTCAAG CCTCAGGACT TCCATCGCTT CCAGCAGACG 
GCCCGGCCCA CCTTCTTTGC CCGAAAGTTC GAAGCCATAG TGAACCAGGA AATCATTGGG 
CAGTTGGACT CTTACCTGTA TGGCAACTAT CCTGCGGGCA CCCCGGGCCT CCGCTCTTAC 
TGGGAGAATG TCTATGACGA ACCAGATGGC ATCCAGAGCC TCAGCGATGT GGCACTCACC 
ATGTATCATT CCTTCATCCG CCTGGGTCTT CGAAGGGCTG AGTCATCGCT ACACACGGAT 
GGGGAGAACA GCTGCAGGTA CTATCCAATG GGCCACCCAG CGTCTGTCCA CCTCTACTTC 
CTGGCTGACC GATTCCAGGG CTTTCTGATC AAGCACCATG TGACCAACCT TGCTGTGAGC 
AAACTGGAGA CACTGGAGAC ATGGATGATG CCAAAGAAAG TCTTCAAGGT CGCAAGTCCC 
CCCAGTGACT TTGGGAGGCT TCAGTTTTCT GAGGTTGGCA CTGACTGGGA TGCCAAGGAG 
AGACTATTCC GGAACTTTGG TGGTCTTTTG GGGCCCATGG ATGAGCCGGT GGGAATGCAG 
AAATGGGGGA AGGGACCCAA TGTGACCGTG ACTGTTATTT GGGTGGATCC TGTCAATGTC 
ATCGCAGCCA CCTATGATAT CCTGATCGAG TCCACTGCGG AATTCACACA CTACAAACCC 
CCTTTGAATC TGCCTCTGAG GCCTGGTGTC TGGACAGTGA AGATTCTCCA TCACTGGGTA 
CCAGTTGCAG AGACCAAATT CCTTGTCGCA CCTCTGACCT TCTCCAACAA GCAGCCCATC 
AAACCAGAGG AGGCTTTGAA GCTGCACAAT GGGCCACCTC GCAGTGCCTA CATGGAGCAG 
AGTTTCCAGA GCCTAAACCC AGTCCTCAGC CTGCACATCA ATCCTGCCCA AGTAGAGCAG 
GCCCGGAAGA ACGCAGCCTT CACTGGGACA GCACTAGAAG CCTGGCTGGA CTCGCTGGTG 
GGTGGGACTT GGACTGCCAT GGACGTCTGT GCCACAGGCC CCACTGCCTG CCCAGTCATG 
CAGACCTGCA GCCAAACAGC CTGGAGTTCC TTCAGCCCTG ATCCCAAGTC AGAGCTGGGT 
GCAGTGAAGC CTGATGGTCG GCTCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006230213.2
CDS	1..2970
Translation	MQEIQRVDTEETDMDYSEAEKDTGCPGTRAPGRRGRWAAPCGEAGAGGSHSACVAGAMVALLQTLCVWNFSSVDSAWRRAARGARAAGSSTAQQQPAAPRRERRDLAAHLPAARGGPGGRAGGGGARGGGPGGARAQQPASRGALAARARDPHPSPLITLETQDGYFSHRPKEKVRTDSNNENSVPKDFENVDNSNFAPRTQKQKHQPELAKKPLSRQKERLQRKLGAQDKGQGQSVLGKGPKEVLPPREKAPGNSSQGKDLSRHSHSRKSGGGGSPETKSDQVPKCDISGKEAISALTRAKSKHCRQEIAETYCRHKLGLLMPEKVARFCPLEGKANKNVQWDEDAVEYMPPNPVRIAFVLVVHGRASRQLQRMFKAIYHKDHFYYIHVDKRSNYLHRQVLQFSRQYDNVRVTSWRMATIWGGASLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIRTNDQLVAFLSRYRDMNFLKSHGRDNARFIRKQGLDRLFLECDTHMWRLGDRRIPEGIAVDGGSDWFLLNRKFVEYVAFSTDDLVTKMKQFYSYTLLPAESFFHTVLENSPHCDTMVDNNLRITNWNRKLGCKCQYKHIVDWCGCSPNDFKPQDFHRFQQTARPTFFARKFEAIVNQEIIGQLDSYLYGNYPAGTPGLRSYWENVYDEPDGIQSLSDVALTMYHSFIRLGLRRAESSLHTDGENSCRYYPMGHPASVHLYFLADRFQGFLIKHHVTNLAVSKLETLETWMMPKKVFKVASPPSDFGRLQFSEVGTDWDAKERLFRNFGGLLGPMDEPVGMQKWGKGPNVTVTVIWVDPVNVIAATYDILIESTAEFTHYKPPLNLPLRPGVWTVKILHHWVPVAETKFLVAPLTFSNKQPIKPEEALKLHNGPPRSAYMEQSFQSLNPVLSLHINPAQVEQARKNAAFTGTALEAWLDSLVGGTWTAMDVCATGPTACPVMQTCSQTAWSSFSPDPKSELGAVKPDGRLR

Target ORF information:

RefSeq Version	XM_006230151.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus xylosyltransferase 1 (Xylt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006230151.2

ORF Insert Sequence:

ATGCAGGAGA TTCAGAGAGT GGACACAGAG GAAACAGATA TGGACTACAG TGAAGCAGAG 
AAAGACACGG GGTGTCCCGG CACCCGCGCC CCCGGGCGCC GCGGAAGATG GGCGGCGCCC 
TGTGGCGAGG CTGGCGCCGG CGGCTCGCAC TCGGCGTGCG TGGCGGGCGC TATGGTGGCG 
CTGCTGCAGA CGCTGTGCGT GTGGAATTTC AGCAGCGTGG ACTCGGCCTG GAGGAGAGCG 
GCGCGCGGGG CGCGAGCGGC CGGGAGCAGC ACAGCGCAGC AGCAGCCCGC GGCCCCGCGC 
CGGGAGCGCA GGGACCTGGC TGCCCATCTG CCCGCAGCCC GCGGAGGACC TGGAGGCCGA 
GCCGGCGGAG GAGGAGCACG GGGAGGCGGC CCCGGAGGAG CCCGCGCACA GCAGCCTGCC 
AGCCGCGGGG CACTAGCCGC CCGGGCGAGG GATCCACACC CAAGTCCACT GATCACCCTA 
GAGACCCAGG ATGGCTACTT CTCTCACCGG CCCAAAGAGA AAGTTCGGAC CGACAGCAAC 
AATGAGAACT CAGTCCCCAA GGACTTTGAG AATGTGGACA ACAGCAACTT CGCACCCAGG 
ACCCAGAAGC AGAAGCACCA GCCAGAGTTG GCAAAGAAGC CCCTCAGCAG GCAGAAAGAG 
CGTTTGCAGA GAAAGCTGGG CGCCCAGGAC AAAGGACAGG GGCAGTCAGT CCTAGGAAAA 
GGCCCCAAGG AGGTGCTGCC TCCTCGGGAA AAAGCCCCAG GCAACAGTAG CCAAGGGAAG 
GATCTCTCAA GACACAGCCA TAGCAGGAAG AGTGGTGGCG GTGGGTCCCC GGAAACTAAG 
TCTGACCAGG TCCCCAAGTG TGACATCTCT GGCAAGGAGG CCATCTCAGC GCTGACCCGC 
GCTAAGTCCA AGCACTGTCG CCAGGAGATT GCAGAAACCT ACTGTCGCCA CAAGCTGGGG 
CTGCTGATGC CAGAGAAGGT GGCTCGATTC TGTCCCCTGG AAGGCAAAGC CAACAAGAAT 
GTCCAGTGGG ATGAGGATGC TGTTGAGTAC ATGCCCCCCA ACCCAGTCAG AATCGCCTTT 
GTCCTGGTGG TCCATGGCCG TGCCTCTCGA CAGCTGCAGC GCATGTTTAA GGCCATCTAC 
CACAAAGACC ATTTCTACTA TATCCATGTG GATAAGCGTT CCAATTACCT GCATCGGCAA 
GTGCTCCAGT TCTCCAGGCA GTACGACAAC GTCCGAGTCA CTTCCTGGAG GATGGCCACC 
ATTTGGGGCG GAGCCAGCCT CCTGTCCACT TACCTGCAGA GCATGCGGGA TCTACTGGAG 
ATGACTGACT GGCCCTGGGA CTTCTTCATC AACCTCAGTG CTGCTGACTA CCCCATCAGG 
ACAAATGACC AGCTCGTAGC ATTTCTTTCC AGATACCGAG ATATGAATTT CCTGAAGTCA 
CATGGTCGGG ACAATGCAAG GTTCATCCGA AAGCAGGGCC TGGACCGTCT CTTCCTGGAG 
TGTGATACAC ACATGTGGCG CCTGGGGGAC CGGCGAATCC CAGAGGGCAT TGCTGTAGAT 
GGCGGCTCTG ATTGGTTCCT GCTAAACCGG AAGTTTGTAG AGTATGTGGC ATTCTCCACA 
GATGACCTGG TGACCAAGAT GAAGCAGTTC TACTCTTATA CCCTCCTCCC TGCTGAGTCC 
TTTTTCCACA CGGTCCTGGA GAACAGCCCC CACTGTGACA CCATGGTGGA TAACAACCTG 
CGCATCACCA ATTGGAACCG CAAGCTGGGC TGCAAGTGCC AGTACAAGCA TATTGTGGAC 
TGGTGTGGCT GCTCTCCCAA TGACTTCAAG CCTCAGGACT TCCATCGCTT CCAGCAGACG 
GCCCGGCCCA CCTTCTTTGC CCGAAAGTTC GAAGCCATAG TGAACCAGGA AATCATTGGG 
CAGTTGGACT CTTACCTGTA TGGCAACTAT CCTGCGGGCA CCCCGGGCCT CCGCTCTTAC 
TGGGAGAATG TCTATGACGA ACCAGATGGC ATCCAGAGCC TCAGCGATGT GGCACTCACC 
ATGTATCATT CCTTCATCCG CCTGGGTCTT CGAAGGGCTG AGTCATCGCT ACACACGGAT 
GGGGAGAACA GCTGCAGGTA CTATCCAATG GGCCACCCAG CGTCTGTCCA CCTCTACTTC 
CTGGCTGACC GATTCCAGGG CTTTCTGATC AAGCACCATG TGACCAACCT TGCTGTGAGC 
AAACTGGAGA CACTGGAGAC ATGGATGATG CCAAAGAAAG TCTTCAAGGT CGCAAGTCCC 
CCCAGTGACT TTGGGAGGCT TCAGTTTTCT GAGGTTGGCA CTGACTGGGA TGCCAAGGAG 
AGACTATTCC GGAACTTTGG TGGTCTTTTG GGGCCCATGG ATGAGCCGGT GGGAATGCAG 
AAATGGGGGA AGGGACCCAA TGTGACCGTG ACTGTTATTT GGGTGGATCC TGTCAATGTC 
ATCGCAGCCA CCTATGATAT CCTGATCGAG TCCACTGCGG AATTCACACA CTACAAACCC 
CCTTTGAATC TGCCTCTGAG GCCTGGTGTC TGGACAGTGA AGATTCTCCA TCACTGGGTA 
CCAGTTGCAG AGACCAAATT CCTTGTCGCA CCTCTGACCT TCTCCAACAA GCAGCCCATC 
AAACCAGAGG AGGCTTTGAA GCTGCACAAT GGGCCACCTC GCAGTGCCTA CATGGAGCAG 
AGTTTCCAGA GCCTAAACCC AGTCCTCAGC CTGCACATCA ATCCTGCCCA AGTAGAGCAG 
GCCCGGAAGA ACGCAGCCTT CACTGGGACA GCACTAGAAG CCTGGCTGGA CTCGCTGGTG 
GGTGGGACTT GGACTGCCAT GGACGTCTGT GCCACAGGCC CCACTGCCTG CCCAGTCATG 
CAGACCTGCA GCCAAACAGC CTGGAGTTCC TTCAGCCCTG ATCCCAAGTC AGAGCTGGGT 
GCAGTGAAGC CTGATGGTCG GCTCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Xylosyltransferase-1 expression is refractory to inhibition by the inflammatory cytokines tumor necrosis factor ? and IL-1? in nucleus pulposus cells: novel regulation by AP-1, Sp1, and Sp3.
The American journal of pathology185(2)485-95(2015 Feb)
Ye W,Zhou J,Markova DZ,Tian Y,Li J,Anderson DG,Shapiro IM,Risbud MV

Forward genetics defines Xylt1 as a key, conserved regulator of early chondrocyte maturation and skeletal length.
Developmental biology385(1)67-82(2014 Jan)
Mis EK,Liem KF Jr,Kong Y,Schwartz NB,Domowicz M,Weatherbee SD

Modulation of xylosyltransferase I expression provides a mechanism regulating glycosaminoglycan chain synthesis during cartilage destruction and repair.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology23(3)813-22(2009 Mar)
Venkatesan N,Barr? L,Magdalou J,Mainard D,Netter P,Fournel-Gigleux S,Ouzzine M

Deoxyribozyme-mediated knockdown of xylosyltransferase-1 mRNA promotes axon growth in the adult rat spinal cord.
Brain : a journal of neurology131(Pt 10)2596-605(2008 Oct)
Hurtado A,Podinin H,Oudega M,Grimpe B

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Xylt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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