Npffr1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Npffr1
Entrez Gene ID	64107
Full Name	neuropeptide FF receptor 1
Synonyms	Gpr147,NPFF1,OT7T022
General protein information	Preferred Names neuropeptide FF receptor 1 Names neuropeptide FF receptor 1 G-protein coupled receptor 147 RFamide-related peptide receptor OT7T022
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20q11
Summary	a G protein-coupled receptor that binds neuropeptide FF and BIBP3226, an anorexigenic Y1 receptor ligand and has a potential role in feeding [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006256485.3	XP_006256547.1	neuropeptide FF receptor 1 isoform X1
XM_017601768.1	XP_017457257.1	neuropeptide FF receptor 1 isoform X2
NM_022291.2	NP_071627.2	neuropeptide FF receptor 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NPFFR1	XP_002698951.1
Pan troglodytes (chimpanzee)	NPFFR1	XP_521501.3
	LOC716688	XP_001107552.2
Xenopus tropicalis (tropical clawed frog)	npffr1	XP_004915843.1
Canis lupus familiaris (dog)	NPFFR1	XP_851935.2
Homo sapiens (human)	NPFFR1	NP_071429.1
Mus musculus (house mouse)	Npffr1	NP_001170982.1
Rattus norvegicus (Norway rat)	Npffr1	NP_071627.2
Xenopus tropicalis (tropical clawed frog)	LOC100491949	XP_004920471.1
Gallus gallus (chicken)	NPFFR1	NP_989693.1
Danio rerio (zebrafish)	npffr1l3	NP_001082858.1
Danio rerio (zebrafish)	npffr1l2	NP_001165168.1

Gene Ontology
Bibliography

Related articles in PubMed

Tubby family proteins are adapters for ciliary trafficking of integral membrane proteins.
Badgandi HB, Hwang SH, Shimada IS, Loriot E, Mukhopadhyay S
The Journal of cell biology216(3)743-760(2017 Mar)

Changes in RFamide-related peptide-1 (RFRP-1)-immunoreactivity during postnatal development and the estrous cycle.
J?rgensen SR, Andersen MD, Overgaard A, Mikkelsen JD
Endocrinology155(11)4402-10(2014 Nov)

Glucocorticoid receptor-mediated regulation of Rfrp (GnIH) and Gpr147 (GnIH-R) synthesis in immortalized hypothalamic neurons.
Gojska NM, Belsham DD
Molecular and cellular endocrinology384(1-2)23-31(2014 Mar)

Expression of gonadotropin-inhibitory hormone receptors in mouse pituitary gonadotroph L?T2 cells and hypothalamic gonadotropin-releasing hormone-producing GT1-7 cells.
Sukhbaatar U, Kanasaki H, Mijiddorj T, Oride A, Miyazaki K
Endocrine journal61(1)25-34(2014)

Developmental and steroidogenic effects on the gene expression of RFamide related peptides and their receptor in the rat brain and pituitary gland.
Quennell JH, Rizwan MZ, Relf HL, Anderson GM
Journal of neuroendocrinology22(4)309-16(2010 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Adult females, as compared with males, have significantly more RFRP-1-ir per cell, and these cells are regulated during the estrous cycle.
Title: Changes in RFamide-related peptide-1 (RFRP-1)-immunoreactivity during postnatal development and the estrous cycle.

Rfrp (GnIH)-expressing neurons serve as potential upstream mediators of stress-induced effects through Gpr147 (GnIH-R)-dependent mechanisms.
Title: Glucocorticoid receptor-mediated regulation of Rfrp (GnIH) and Gpr147 (GnIH-R) synthesis in immortalized hypothalamic neurons.

two central controllers of reproductive function, gonadotropin-releasing hormone (GnRH) and kisspeptin, stimulate the expression of Gonadotropin-inhibitory hormone (GnIH) receptors in pituitary gonadotroph cells and hypothalamic GnRH neurons
Title: Expression of gonadotropin-inhibitory hormone receptors in mouse pituitary gonadotroph L?T2 cells and hypothalamic gonadotropin-releasing hormone-producing GT1-7 cells.

RFRP-1 injected into the central nucleus of the rat amygdala decreases food intake. This is a NPFF-1 linked effect, because it could be prevented by NPFF-receptor antagonist RF9.
Title: Microinjection of RFRP-1 in the central nucleus of amygdala decreases food intake in the rat.

Developmental and steroidogenic effects on the gene expression of RFamide related peptides and their receptor in the rat brain and pituitary gland.
Title: Developmental and steroidogenic effects on the gene expression of RFamide related peptides and their receptor in the rat brain and pituitary gland.

The following Npffr1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Npffr1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa05636	NM_022291.2 Latest version!	Rattus norvegicus neuropeptide FF receptor 1 (Npffr1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa39483	XM_006256485.3 Latest version!	Rattus norvegicus neuropeptide FF receptor 1 (Npffr1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa60185	XM_017601768.1 Latest version!	Rattus norvegicus neuropeptide FF receptor 1 (Npffr1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Npffr1 ( NM_022291.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071627.2 Rattus norvegicus neuropeptide FF receptor 1 (Npffr1), mRNA.

Price & Availability↑

CloneID	ORa05636
Clone ID Related Accession (Same CDS sequence)	NM_022291.2
Accession Version	NM_022291.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	neuropeptide FF receptor 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07072835.1 and AC139981.3. On Apr 14, 2010 this sequence version replaced NM_022291.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGCGG AGCCCTCCCA GCCTCCCAAC GGCAGCTGGC CCCTGGGTCA GAACGGGAGT 
GATGTGGAGA CCAGCATAGC AACCAGCCTC ACCTTCTCCT CCTACTACCA ACACTCCTCT 
CCGGTGGCAG CCATGTTCAT CGCGGCCTAC GTGCTCATCT TCCTCCTCTG CATGGTGGGC 
AACACCCTGG TCTGCTTCAT TGTGCTCAAG AACCGGCACA TGCGCACTGT CACCAACATG 
TTTATCCTCA ACCTGGCCGT CAGCGACCTG CTGGTGGGCA TCTTCTGCAT GCCCACAACC 
CTTGTGGACA ACCTTATCAC TGGTTGGCCT TTTGACAACG CCACATGCAA GATGAGCGGC 
TTGGTGCAGG GCATGTCCGT GTCTGCATCG GTTTTCACAC TGGTGGCCAT CGCTGTGGAA 
AGGTTCCGCT GCATCGTGCA CCCTTTCCGC GAGAAGCTGA CCCTTCGGAA GGCGCTGTTC 
ACCATCGCGG TGATCTGGGC TCTGGCGCTG CTCATCATGT GTCCCTCGGC GGTCACTCTG 
ACAGTCACCC GAGAGGAGCA TCACTTCATG CTGGATGCTC GTAACCGCTC CTACCCGCTC 
TACTCGTGCT GGGAGGCCTG GCCCGAGAAG GGCATGCGCA AGGTCTACAC CGCGGTGCTC 
TTCGCGCACA TCTACCTGGT GCCGCTGGCG CTCATCGTAG TGATGTACGT GCGCATCGCG 
CGCAAGCTAT GCCAGGCCCC CGGTCCTGCG CGCGACACGG AGGAGGCGGT GGCCGAGGGT 
GGCCGCACTT CGCGCCGTAG GGCCCGCGTG GTGCACATGC TGGTCATGGT GGCGCTCTTC 
TTCACGTTGT CCTGGCTGCC ACTCTGGGTG CTGCTGCTGC TCATCGACTA TGGGGAGCTG 
AGCGAGCTGC AACTGCACCT GCTGTCGGTC TACGCCTTCC CCTTGGCACA CTGGCTGGCC 
TTCTTCCACA GCAGCGCCAA CCCCATCATC TACGGCTACT TCAACGAGAA CTTCCGCCGC 
GGCTTCCAGG CTGCCTTCCG TGCACAGCTC TGCTGGCCTC CCTGGGCCGC CCACAAGCAA 
GCCTACTCGG AGCGGCCCAA CCGCCTCCTG CGCAGGCGGG TGGTGGTGGA CGTGCAACCC 
AGCGACTCCG GCCTGCCATC AGAGTCTGGC CCCAGCAGCG GGGTCCCAGG GCCTGGCCGG 
CTGCCACTGC GAAATGGGCG TGTGGCCCAT CAGGATGGCC CGGGGGAAGG GCCAGGCTGC 
AACCACATGC CCCTCACCAT CCCGGCCTGG AACATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071627.2
CDS	9..1307
Translation	MEAEPSQPPNGSWPLGQNGSDVETSIATSLTFSSYYQHSSPVAAMFIAAYVLIFLLCMVGNTLVCFIVLKNRHMRTVTNMFILNLAVSDLLVGIFCMPTTLVDNLITGWPFDNATCKMSGLVQGMSVSASVFTLVAIAVERFRCIVHPFREKLTLRKALFTIAVIWALALLIMCPSAVTLTVTREEHHFMLDARNRSYPLYSCWEAWPEKGMRKVYTAVLFAHIYLVPLALIVVMYVRIARKLCQAPGPARDTEEAVAEGGRTSRRRARVVHMLVMVALFFTLSWLPLWVLLLLIDYGELSELQLHLLSVYAFPLAHWLAFFHSSANPIIYGYFNENFRRGFQAAFRAQLCWPPWAAHKQAYSERPNRLLRRRVVVDVQPSDSGLPSESGPSSGVPGPGRLPLRNGRVAHQDGPGEGPGCNHMPLTIPAWNI

Target ORF information:

RefSeq Version	NM_022291.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neuropeptide FF receptor 1 (Npffr1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022291.2

ORF Insert Sequence:

ATGGAGGCGG AGCCCTCCCA GCCTCCCAAC GGCAGCTGGC CCCTGGGTCA GAACGGGAGT 
GATGTGGAGA CCAGCATAGC AACCAGCCTC ACCTTCTCCT CCTACTACCA ACACTCCTCT 
CCGGTGGCAG CCATGTTCAT CGCGGCCTAC GTGCTCATCT TCCTCCTCTG CATGGTGGGC 
AACACCCTGG TCTGCTTCAT TGTGCTCAAG AACCGGCACA TGCGCACTGT CACCAACATG 
TTTATCCTCA ACCTGGCCGT CAGCGACCTG CTGGTGGGCA TCTTCTGCAT GCCCACAACC 
CTTGTGGACA ACCTTATCAC TGGTTGGCCT TTTGACAACG CCACATGCAA GATGAGCGGC 
TTGGTGCAGG GCATGTCCGT GTCTGCATCG GTTTTCACAC TGGTGGCCAT CGCTGTGGAA 
AGGTTCCGCT GCATCGTGCA CCCTTTCCGC GAGAAGCTGA CCCTTCGGAA GGCGCTGTTC 
ACCATCGCGG TGATCTGGGC TCTGGCGCTG CTCATCATGT GTCCCTCGGC GGTCACTCTG 
ACAGTCACCC GAGAGGAGCA TCACTTCATG CTGGATGCTC GTAACCGCTC CTACCCGCTC 
TACTCGTGCT GGGAGGCCTG GCCCGAGAAG GGCATGCGCA AGGTCTACAC CGCGGTGCTC 
TTCGCGCACA TCTACCTGGT GCCGCTGGCG CTCATCGTAG TGATGTACGT GCGCATCGCG 
CGCAAGCTAT GCCAGGCCCC CGGTCCTGCG CGCGACACGG AGGAGGCGGT GGCCGAGGGT 
GGCCGCACTT CGCGCCGTAG GGCCCGCGTG GTGCACATGC TGGTCATGGT GGCGCTCTTC 
TTCACGTTGT CCTGGCTGCC ACTCTGGGTG CTGCTGCTGC TCATCGACTA TGGGGAGCTG 
AGCGAGCTGC AACTGCACCT GCTGTCGGTC TACGCCTTCC CCTTGGCACA CTGGCTGGCC 
TTCTTCCACA GCAGCGCCAA CCCCATCATC TACGGCTACT TCAACGAGAA CTTCCGCCGC 
GGCTTCCAGG CTGCCTTCCG TGCACAGCTC TGCTGGCCTC CCTGGGCCGC CCACAAGCAA 
GCCTACTCGG AGCGGCCCAA CCGCCTCCTG CGCAGGCGGG TGGTGGTGGA CGTGCAACCC 
AGCGACTCCG GCCTGCCATC AGAGTCTGGC CCCAGCAGCG GGGTCCCAGG GCCTGGCCGG 
CTGCCACTGC GAAATGGGCG TGTGGCCCAT CAGGATGGCC CGGGGGAAGG GCCAGGCTGC 
AACCACATGC CCCTCACCAT CCCGGCCTGG AACATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Npffr1 ( XM_006256485.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256547.1 Rattus norvegicus neuropeptide FF receptor 1 (Npffr1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa39483
Clone ID Related Accession (Same CDS sequence)	XM_006256485.3
Accession Version	XM_006256485.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neuropeptide FF receptor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256485.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAGGCA AGCAGTCTGA GCTGCATCAC CATCCAAAGG TTGGCTTTTC AGTGGTGAAA 
AGCCAATTAA CTCTTCTTTG TATTGGTCCT GACTCTTGTG CTCCTTTCGC CCCAGCGGAG 
CCCTCCCAGC CTCCCAACGG CAGCTGGCCC CTGGGTCAGA ACGGGAGTGA TGTGGAGACC 
AGCATAGCAA CCAGCCTCAC CTTCTCCTCC TACTACCAAC ACTCCTCTCC GGTGGCAGCC 
ATGTTCATCG CGGCCTACGT GCTCATCTTC CTCCTCTGCA TGGTGGGCAA CACCCTGGTC 
TGCTTCATTG TGCTCAAGAA CCGGCACATG CGCACTGTCA CCAACATGTT TATCCTCAAC 
CTGGCCGTCA GCGACCTGCT GGTGGGCATC TTCTGCATGC CCACAACCCT TGTGGACAAC 
CTTATCACTG GTTGGCCTTT TGACAACGCC ACATGCAAGA TGAGCGGCTT GGTGCAGGGC 
ATGTCCGTGT CTGCATCGGT TTTCACACTG GTGGCCATCG CTGTGGAAAG GTTCCGCTGC 
ATCGTGCACC CTTTCCGCGA GAAGCTGACC CTTCGGAAGG CGCTGTTCAC CATCGCGGTG 
ATCTGGGCTC TGGCGCTGCT CATCATGTGT CCCTCGGCGG TCACTCTGAC AGTCACCCGA 
GAGGAGCATC ACTTCATGCT GGATGCTCGT AACCGCTCCT ACCCGCTCTA CTCGTGCTGG 
GAGGCCTGGC CCGAGAAGGG CATGCGCAAG GTCTACACCG CGGTGCTCTT CGCGCACATC 
TACCTGGTGC CGCTGGCGCT CATCGTAGTG ATGTACGTGC GCATCGCGCG CAAGCTATGC 
CAGGCCCCCG GTCCTGCGCG CGACACGGAG GAGGCGGTGG CCGAGGGTGG CCGCACTTCG 
CGCCGTAGGG CCCGCGTGGT GCACATGCTG GTCATGGTGG CGCTCTTCTT CACGTTGTCC 
TGGCTGCCAC TCTGGGTGCT GCTGCTGCTC ATCGACTATG GGGAGCTGAG CGAGCTGCAA 
CTGCACCTGC TGTCGGTCTA CGCCTTCCCC TTGGCACACT GGCTGGCCTT CTTCCACAGC 
AGCGCCAACC CCATCATCTA CGGCTACTTC AACGAGAACT TCCGCCGCGG CTTCCAGGCT 
GCCTTCCGTG CACAGCTCTG CTGGCCTCCC TGGGCCGCCC ACAAGCAAGC CTACTCGGAG 
CGGCCCAACC GCCTCCTGCG CAGGCGGGTG GTGGTGGACG TGCAACCCAG CGACTCCGGC 
CTGCCATCAG AGTCTGGCCC CAGCAGCGGG GTCCCAGGGC CTGGCCGGCT GCCACTGCGA 
AATGGGCGTG TGGCCCATCA GGATGGCCCG GGGGAAGGGC CAGGCTGCAA CCACATGCCC 
CTCACCATCC CGGCCTGGAA CATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256547.1
CDS	55..1461
Translation	MLGKQSELHHHPKVGFSVVKSQLTLLCIGPDSCAPFAPAEPSQPPNGSWPLGQNGSDVETSIATSLTFSSYYQHSSPVAAMFIAAYVLIFLLCMVGNTLVCFIVLKNRHMRTVTNMFILNLAVSDLLVGIFCMPTTLVDNLITGWPFDNATCKMSGLVQGMSVSASVFTLVAIAVERFRCIVHPFREKLTLRKALFTIAVIWALALLIMCPSAVTLTVTREEHHFMLDARNRSYPLYSCWEAWPEKGMRKVYTAVLFAHIYLVPLALIVVMYVRIARKLCQAPGPARDTEEAVAEGGRTSRRRARVVHMLVMVALFFTLSWLPLWVLLLLIDYGELSELQLHLLSVYAFPLAHWLAFFHSSANPIIYGYFNENFRRGFQAAFRAQLCWPPWAAHKQAYSERPNRLLRRRVVVDVQPSDSGLPSESGPSSGVPGPGRLPLRNGRVAHQDGPGEGPGCNHMPLTIPAWNI

Target ORF information:

RefSeq Version	XM_006256485.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neuropeptide FF receptor 1 (Npffr1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256485.3

ORF Insert Sequence:

ATGTTAGGCA AGCAGTCTGA GCTGCATCAC CATCCAAAGG TTGGCTTTTC AGTGGTGAAA 
AGCCAATTAA CTCTTCTTTG TATTGGTCCT GACTCTTGTG CTCCTTTCGC CCCAGCGGAG 
CCCTCCCAGC CTCCCAACGG CAGCTGGCCC CTGGGTCAGA ACGGGAGTGA TGTGGAGACC 
AGCATAGCAA CCAGCCTCAC CTTCTCCTCC TACTACCAAC ACTCCTCTCC GGTGGCAGCC 
ATGTTCATCG CGGCCTACGT GCTCATCTTC CTCCTCTGCA TGGTGGGCAA CACCCTGGTC 
TGCTTCATTG TGCTCAAGAA CCGGCACATG CGCACTGTCA CCAACATGTT TATCCTCAAC 
CTGGCCGTCA GCGACCTGCT GGTGGGCATC TTCTGCATGC CCACAACCCT TGTGGACAAC 
CTTATCACTG GTTGGCCTTT TGACAACGCC ACATGCAAGA TGAGCGGCTT GGTGCAGGGC 
ATGTCCGTGT CTGCATCGGT TTTCACACTG GTGGCCATCG CTGTGGAAAG GTTCCGCTGC 
ATCGTGCACC CTTTCCGCGA GAAGCTGACC CTTCGGAAGG CGCTGTTCAC CATCGCGGTG 
ATCTGGGCTC TGGCGCTGCT CATCATGTGT CCCTCGGCGG TCACTCTGAC AGTCACCCGA 
GAGGAGCATC ACTTCATGCT GGATGCTCGT AACCGCTCCT ACCCGCTCTA CTCGTGCTGG 
GAGGCCTGGC CCGAGAAGGG CATGCGCAAG GTCTACACCG CGGTGCTCTT CGCGCACATC 
TACCTGGTGC CGCTGGCGCT CATCGTAGTG ATGTACGTGC GCATCGCGCG CAAGCTATGC 
CAGGCCCCCG GTCCTGCGCG CGACACGGAG GAGGCGGTGG CCGAGGGTGG CCGCACTTCG 
CGCCGTAGGG CCCGCGTGGT GCACATGCTG GTCATGGTGG CGCTCTTCTT CACGTTGTCC 
TGGCTGCCAC TCTGGGTGCT GCTGCTGCTC ATCGACTATG GGGAGCTGAG CGAGCTGCAA 
CTGCACCTGC TGTCGGTCTA CGCCTTCCCC TTGGCACACT GGCTGGCCTT CTTCCACAGC 
AGCGCCAACC CCATCATCTA CGGCTACTTC AACGAGAACT TCCGCCGCGG CTTCCAGGCT 
GCCTTCCGTG CACAGCTCTG CTGGCCTCCC TGGGCCGCCC ACAAGCAAGC CTACTCGGAG 
CGGCCCAACC GCCTCCTGCG CAGGCGGGTG GTGGTGGACG TGCAACCCAG CGACTCCGGC 
CTGCCATCAG AGTCTGGCCC CAGCAGCGGG GTCCCAGGGC CTGGCCGGCT GCCACTGCGA 
AATGGGCGTG TGGCCCATCA GGATGGCCCG GGGGAAGGGC CAGGCTGCAA CCACATGCCC 
CTCACCATCC CGGCCTGGAA CATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Npffr1 ( XM_017601768.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017457257.1 Rattus norvegicus neuropeptide FF receptor 1 (Npffr1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa60185
Clone ID Related Accession (Same CDS sequence)	XM_017601768.1
Accession Version	XM_017601768.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	neuropeptide FF receptor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005119.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA AGGTTAATTA TGGATATGTG GCTGAGAACT GCCAGAGGCA GATGTATCTG 
GGAGAGTCCA GAGTGGCCAT GATCTGGAAT CATGTGGGGA GAGGGGGAGG GGAAGGGAGA 
GAGAGGAACG AAGGACAACA GCCAAGAGGC TGTGCAAAAG AAAGGTTCCG CTGCATCGTG 
CACCCTTTCC GCGAGAAGCT GACCCTTCGG AAGGCGCTGT TCACCATCGC GGTGATCTGG 
GCTCTGGCGC TGCTCATCAT GTGTCCCTCG GCGGTCACTC TGACAGTCAC CCGAGAGGAG 
CATCACTTCA TGCTGGATGC TCGTAACCGC TCCTACCCGC TCTACTCGTG CTGGGAGGCC 
TGGCCCGAGA AGGGCATGCG CAAGGTCTAC ACCGCGGTGC TCTTCGCGCA CATCTACCTG 
GTGCCGCTGG CGCTCATCGT AGTGATGTAC GTGCGCATCG CGCGCAAGCT ATGCCAGGCC 
CCCGGTCCTG CGCGCGACAC GGAGGAGGCG GTGGCCGAGG GTGGCCGCAC TTCGCGCCGT 
AGGGCCCGCG TGGTGCACAT GCTGGTCATG GTGGCGCTCT TCTTCACGTT GTCCTGGCTG 
CCACTCTGGG TGCTGCTGCT GCTCATCGAC TATGGGGAGC TGAGCGAGCT GCAACTGCAC 
CTGCTGTCGG TCTACGCCTT CCCCTTGGCA CACTGGCTGG CCTTCTTCCA CAGCAGCGCC 
AACCCCATCA TCTACGGCTA CTTCAACGAG AACTTCCGCC GCGGCTTCCA GGCTGCCTTC 
CGTGCACAGC TCTGCTGGCC TCCCTGGGCC GCCCACAAGC AAGCCTACTC GGAGCGGCCC 
AACCGCCTCC TGCGCAGGCG GGTGGTGGTG GACGTGCAAC CCAGCGACTC CGGCCTGCCA 
TCAGAGTCTG GCCCCAGCAG CGGGGTCCCA GGGCCTGGCC GGCTGCCACT GCGAAATGGG 
CGTGTGGCCC ATCAGGATGG CCCGGGGGAA GGGCCAGGCT GCAACCACAT GCCCCTCACC 
ATCCCGGCCT GGAACATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017457257.1
CDS	156..1196
Translation	MEEKVNYGYVAENCQRQMYLGESRVAMIWNHVGRGGGEGRERNEGQQPRGCAKERFRCIVHPFREKLTLRKALFTIAVIWALALLIMCPSAVTLTVTREEHHFMLDARNRSYPLYSCWEAWPEKGMRKVYTAVLFAHIYLVPLALIVVMYVRIARKLCQAPGPARDTEEAVAEGGRTSRRRARVVHMLVMVALFFTLSWLPLWVLLLLIDYGELSELQLHLLSVYAFPLAHWLAFFHSSANPIIYGYFNENFRRGFQAAFRAQLCWPPWAAHKQAYSERPNRLLRRRVVVDVQPSDSGLPSESGPSSGVPGPGRLPLRNGRVAHQDGPGEGPGCNHMPLTIPAWNI

Target ORF information:

RefSeq Version	XM_017601768.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neuropeptide FF receptor 1 (Npffr1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017601768.1

ORF Insert Sequence:

ATGGAGGAGA AGGTTAATTA TGGATATGTG GCTGAGAACT GCCAGAGGCA GATGTATCTG 
GGAGAGTCCA GAGTGGCCAT GATCTGGAAT CATGTGGGGA GAGGGGGAGG GGAAGGGAGA 
GAGAGGAACG AAGGACAACA GCCAAGAGGC TGTGCAAAAG AAAGGTTCCG CTGCATCGTG 
CACCCTTTCC GCGAGAAGCT GACCCTTCGG AAGGCGCTGT TCACCATCGC GGTGATCTGG 
GCTCTGGCGC TGCTCATCAT GTGTCCCTCG GCGGTCACTC TGACAGTCAC CCGAGAGGAG 
CATCACTTCA TGCTGGATGC TCGTAACCGC TCCTACCCGC TCTACTCGTG CTGGGAGGCC 
TGGCCCGAGA AGGGCATGCG CAAGGTCTAC ACCGCGGTGC TCTTCGCGCA CATCTACCTG 
GTGCCGCTGG CGCTCATCGT AGTGATGTAC GTGCGCATCG CGCGCAAGCT ATGCCAGGCC 
CCCGGTCCTG CGCGCGACAC GGAGGAGGCG GTGGCCGAGG GTGGCCGCAC TTCGCGCCGT 
AGGGCCCGCG TGGTGCACAT GCTGGTCATG GTGGCGCTCT TCTTCACGTT GTCCTGGCTG 
CCACTCTGGG TGCTGCTGCT GCTCATCGAC TATGGGGAGC TGAGCGAGCT GCAACTGCAC 
CTGCTGTCGG TCTACGCCTT CCCCTTGGCA CACTGGCTGG CCTTCTTCCA CAGCAGCGCC 
AACCCCATCA TCTACGGCTA CTTCAACGAG AACTTCCGCC GCGGCTTCCA GGCTGCCTTC 
CGTGCACAGC TCTGCTGGCC TCCCTGGGCC GCCCACAAGC AAGCCTACTC GGAGCGGCCC 
AACCGCCTCC TGCGCAGGCG GGTGGTGGTG GACGTGCAAC CCAGCGACTC CGGCCTGCCA 
TCAGAGTCTG GCCCCAGCAG CGGGGTCCCA GGGCCTGGCC GGCTGCCACT GCGAAATGGG 
CGTGTGGCCC ATCAGGATGG CCCGGGGGAA GGGCCAGGCT GCAACCACAT GCCCCTCACC 
ATCCCGGCCT GGAACATTTG A