Vps33b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vps33b
Entrez Gene ID	64060
Full Name	VPS33B, late endosome and lysosome associated
General protein information	Preferred Names VPS33B, late endosome and lysosome associated Names vacuolar protein sorting-associated protein 33B r-vps33b vacuolar protein sorting 33 homolog B vacuolar protein sorting 33B vacuolar protein sorting homolog r-vps33b
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q31
Summary	role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022286.2	NP_071622.1	vacuolar protein sorting-associated protein 33B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	VPS33B	XP_510601.2
Mus musculus (house mouse)	Vps33b	NP_835171.2
Caenorhabditis elegans (roundworm)	vps-33.2	NP_495342.2
Canis lupus familiaris (dog)	VPS33B	XP_536197.2
Danio rerio (zebrafish)	vps33b	NP_001014370.1
	VPS33B	XP_001097946.1
Gallus gallus (chicken)	VPS33B	NP_001026519.1
Bos taurus (cattle)	VPS33B	NP_001039749.1
Homo sapiens (human)	VPS33B	NP_061138.3
Rattus norvegicus (Norway rat)	Vps33b	NP_071622.1
Xenopus tropicalis (tropical clawed frog)	vps33b	NP_001096504.1

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis.
Banushi B, Forneris F, Straatman-Iwanowska A, Strange A, Lyne AM, Rogerson C, Burden JJ, Heywood WE, Hanley J, Doykov I, Straatman KR, Smith H, Bem D, Kriston-Vizi J, Ariceta G, Risteli M, Wang C, Ardill RE, Zaniew M, Latka-Grot J, Waddington SN, Howe SJ, Ferraro F, Gjinovci A, Lawrence S, Marsh M, Girolami M, Bozec L, Mills K, Gissen P
Nature communications712111(2016 Jul)

VPS33B regulates protein sorting into and maturation of ?-granule progenitor organelles in mouse megakaryocytes.
Bem D, Smith H, Banushi B, Burden JJ, White IJ, Hanley J, Jeremiah N, Rieux-Laucat F, Bettels R, Ariceta G, Mumford AD, Thomas SG, Watson SP, Gissen P
Blood126(2)133-43(2015 Jul)

Recruitment of VPS33A to HOPS by VPS16 Is Required for Lysosome Fusion with Endosomes and Autophagosomes.
Wartosch L, G?nesdogan U, Graham SC, Luzio JP
Traffic (Copenhagen, Denmark)16(7)727-42(2015 Jul)

Vps33b pathogenic mutations preferentially affect VIPAS39/SPE-39-positive endosomes.
Tornieri K, Zlatic SA, Mullin AP, Werner E, Harrison R, L'hernault SW, Faundez V
Human molecular genetics22(25)5215-28(2013 Dec)

Clathrin-dependent mechanisms modulate the subcellular distribution of class C Vps/HOPS tether subunits in polarized and nonpolarized cells.
Zlatic SA, Tornieri K, L'Hernault SW, Faundez V
Molecular biology of the cell22(10)1699-715(2011 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Vps33b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vps33b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05622	NM_022286.2 Latest version!	Rattus norvegicus VPS33B, late endosome and lysosome associated (Vps33b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vps33b ( NM_022286.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071622.1 Rattus norvegicus VPS33B, late endosome and lysosome associated (Vps33b), mRNA.

Price & Availability↑

CloneID	ORa05622
Clone ID Related Accession (Same CDS sequence)	NM_022286.2
Accession Version	NM_022286.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	vacuolar protein sorting-associated protein 33B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC081707.1. On Sep 16, 2004 this sequence version replaced NM_022286.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC081707.1, U35245.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCTTTCC CCCATCGACT GGACGCCCCT GAGCTTCCCG ACTTCTCTAT GCTCAAGAGG 
CTGGCTCGGG ACCAGCTCAT CTATCTGCTA GAACAGCTTC CTGGGAAAAA GGATTTGTTC 
ATTGAGGCAG ATCTCATGAG CCCTTTGGAT CGAATTGCTA ACGTCTCTAT CCTGAAGCAA 
CATGAAGTAG ACAAGCTGTA CAAGGTGGAA AACAAACTTA CTCTCAGCTC CAATGAACAA 
TTGTGCTTCT TGGTCAGACC TCGAATCAAG ACTATGCGCT ACATTGCCAA TCTTGTCAAT 
GCTGACAAAC TGGCTGGCCG AGTTAGAAAA TACAAAATCA TCTTAAGTCC TCAGAAGTTT 
TATGCATGTG AGATGGTGCT TGAGGAAGAG GGAGTCTATG GAGATGTGAG CTGTGATGAA 
TGGGCCTTTT CTCTGCTGCC TCTTGATGTG GATCTGCTGA GCATGGAACT GCCAGAATTT 
TTCAGGGATT ACTTCCTGGA AGGGGATCAG CGTTGGATCA ACACGGTGGC TCAGGCCTTG 
CACCTTCTCA GTACTCTCTA TGGGCCCTTC CCTAACTGCT ATGGCATTGG CAGATGCACG 
AAGATGTCAT ATGACCTGTG GAGGAAACTG GAGGAGGAAG AGGACAGTGA AACGAAAGGT 
CGGAGACCAG AGATTGGACA CATCTTTCTC TTGGACAGAG ATGTGGACTT TGTGACAGCA 
CTTTGCTCCC AAGTGGTTTA TGAGGGCTTG GTAGATGACA CCTTCCGAAT CAAGTGTGGA 
AGTGTCGACT TTGGCCCAGA AGTCACATCC TCTGACAAGA GCCTGAAGGT GCTGCTCAAC 
GCTGAGGACA AGGTGTTCAG TGAGATCCGC AATGAGCACT TCTCCAATGT CTTTGGCTTC 
TTGAGCCAGA AAGCCCGGAA CTTGCAGGCT CAATATGACC GCCGGAGAGG TATGGACATA 
AAGCAGATGA AGAACTTCGT GTCCCAAGAG CTCAAGGGAC TGAAACAGGA GCACCGCCTT 
CTGAGTCTCC ATATTGGAGC TTGTGAATCA ATCATGAAGA AGAAAACCAA GCAGGACTTC 
CAGGAGCTAA TCAAGACCGA GCATGCACTG CTGGAGGGCT TCAACATTCG AGAGAGCACT 
AGCTACATTG AAGAACACAT AGACCGGCAG GTGTCGCCCA TAGAAAGCCT ACGCCTCATG 
TGCCTTTTGT CCATCACTGA GAATGGTTTG ATTCCCAAGG ATTATCGGTC CCTGAAAACA 
CAGTATCTGC AGAGCTACGG CCCCGAGCAC CTGCTAACCT TCTCCAACCT TCGGAGAGCC 
GGGCTTCTAA CAGAGCAGGC TTCGGGGGAC ACCCTCACTG CAGTAGAGAA TAAAGTGAGC 
AAGCTGGTGA CTGACAAGGC CGCAGGAAAA ATCACTGATG CCTTCAGTTC TCTGGCCAAG 
AGGAGCAATT TTCGTGCCAT CAGCAAAAAG CTGAATTTGA TCCCACGTGT AGATGGGGAG 
TATGACCTGA AAGTGCCCCG GGACATGGCT TACGTCTTCA GTGGCGCCTA CGTGCCTCTG 
AGCTGCCGAA TCATTGAGCA GGTGCTGGAC CGGCGGAGTT GGCAGGGCCT AGATGAAGTG 
GTCCGGCTGC TAAACTGCAG TGACTTTGCA TTCACAGACA TGGCTAAGGA AGACAAGGCT 
TCCAGTGAGT CACTCCGCCT CATCTTGGTG GTGTTCTTGG GGGGCTGCAC GTTCTCAGAG 
ATATCAGCCC TGCGCTTCCT GGGTAGAGAG AAAGGGTACA GATTTATTTT TCTGACAACT 
GCTGTTACAA ACAGTGCTCG CCTCATGGAA GCCATGAGTG AGGTGAAATC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071622.1
CDS	312..2165
Translation	MAFPHRLDAPELPDFSMLKRLARDQLIYLLEQLPGKKDLFIEADLMSPLDRIANVSILKQHEVDKLYKVENKLTLSSNEQLCFLVRPRIKTMRYIANLVNADKLAGRVRKYKIILSPQKFYACEMVLEEEGVYGDVSCDEWAFSLLPLDVDLLSMELPEFFRDYFLEGDQRWINTVAQALHLLSTLYGPFPNCYGIGRCTKMSYDLWRKLEEEEDSETKGRRPEIGHIFLLDRDVDFVTALCSQVVYEGLVDDTFRIKCGSVDFGPEVTSSDKSLKVLLNAEDKVFSEIRNEHFSNVFGFLSQKARNLQAQYDRRRGMDIKQMKNFVSQELKGLKQEHRLLSLHIGACESIMKKKTKQDFQELIKTEHALLEGFNIRESTSYIEEHIDRQVSPIESLRLMCLLSITENGLIPKDYRSLKTQYLQSYGPEHLLTFSNLRRAGLLTEQASGDTLTAVENKVSKLVTDKAAGKITDAFSSLAKRSNFRAISKKLNLIPRVDGEYDLKVPRDMAYVFSGAYVPLSCRIIEQVLDRRSWQGLDEVVRLLNCSDFAFTDMAKEDKASSESLRLILVVFLGGCTFSEISALRFLGREKGYRFIFLTTAVTNSARLMEAMSEVKS

Target ORF information:

RefSeq Version	NM_022286.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus VPS33B, late endosome and lysosome associated (Vps33b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022286.2

ORF Insert Sequence:

ATGGCTTTCC CCCATCGACT GGACGCCCCT GAGCTTCCCG ACTTCTCTAT GCTCAAGAGG 
CTGGCTCGGG ACCAGCTCAT CTATCTGCTA GAACAGCTTC CTGGGAAAAA GGATTTGTTC 
ATTGAGGCAG ATCTCATGAG CCCTTTGGAT CGAATTGCTA ACGTCTCTAT CCTGAAGCAA 
CATGAAGTAG ACAAGCTGTA CAAGGTGGAA AACAAACTTA CTCTCAGCTC CAATGAACAA 
TTGTGCTTCT TGGTCAGACC TCGAATCAAG ACTATGCGCT ACATTGCCAA TCTTGTCAAT 
GCTGACAAAC TGGCTGGCCG AGTTAGAAAA TACAAAATCA TCTTAAGTCC TCAGAAGTTT 
TATGCATGTG AGATGGTGCT TGAGGAAGAG GGAGTCTATG GAGATGTGAG CTGTGATGAA 
TGGGCCTTTT CTCTGCTGCC TCTTGATGTG GATCTGCTGA GCATGGAACT GCCAGAATTT 
TTCAGGGATT ACTTCCTGGA AGGGGATCAG CGTTGGATCA ACACGGTGGC TCAGGCCTTG 
CACCTTCTCA GTACTCTCTA TGGGCCCTTC CCTAACTGCT ATGGCATTGG CAGATGCACG 
AAGATGTCAT ATGACCTGTG GAGGAAACTG GAGGAGGAAG AGGACAGTGA AACGAAAGGT 
CGGAGACCAG AGATTGGACA CATCTTTCTC TTGGACAGAG ATGTGGACTT TGTGACAGCA 
CTTTGCTCCC AAGTGGTTTA TGAGGGCTTG GTAGATGACA CCTTCCGAAT CAAGTGTGGA 
AGTGTCGACT TTGGCCCAGA AGTCACATCC TCTGACAAGA GCCTGAAGGT GCTGCTCAAC 
GCTGAGGACA AGGTGTTCAG TGAGATCCGC AATGAGCACT TCTCCAATGT CTTTGGCTTC 
TTGAGCCAGA AAGCCCGGAA CTTGCAGGCT CAATATGACC GCCGGAGAGG TATGGACATA 
AAGCAGATGA AGAACTTCGT GTCCCAAGAG CTCAAGGGAC TGAAACAGGA GCACCGCCTT 
CTGAGTCTCC ATATTGGAGC TTGTGAATCA ATCATGAAGA AGAAAACCAA GCAGGACTTC 
CAGGAGCTAA TCAAGACCGA GCATGCACTG CTGGAGGGCT TCAACATTCG AGAGAGCACT 
AGCTACATTG AAGAACACAT AGACCGGCAG GTGTCGCCCA TAGAAAGCCT ACGCCTCATG 
TGCCTTTTGT CCATCACTGA GAATGGTTTG ATTCCCAAGG ATTATCGGTC CCTGAAAACA 
CAGTATCTGC AGAGCTACGG CCCCGAGCAC CTGCTAACCT TCTCCAACCT TCGGAGAGCC 
GGGCTTCTAA CAGAGCAGGC TTCGGGGGAC ACCCTCACTG CAGTAGAGAA TAAAGTGAGC 
AAGCTGGTGA CTGACAAGGC CGCAGGAAAA ATCACTGATG CCTTCAGTTC TCTGGCCAAG 
AGGAGCAATT TTCGTGCCAT CAGCAAAAAG CTGAATTTGA TCCCACGTGT AGATGGGGAG 
TATGACCTGA AAGTGCCCCG GGACATGGCT TACGTCTTCA GTGGCGCCTA CGTGCCTCTG 
AGCTGCCGAA TCATTGAGCA GGTGCTGGAC CGGCGGAGTT GGCAGGGCCT AGATGAAGTG 
GTCCGGCTGC TAAACTGCAG TGACTTTGCA TTCACAGACA TGGCTAAGGA AGACAAGGCT 
TCCAGTGAGT CACTCCGCCT CATCTTGGTG GTGTTCTTGG GGGGCTGCAC GTTCTCAGAG 
ATATCAGCCC TGCGCTTCCT GGGTAGAGAG AAAGGGTACA GATTTATTTT TCTGACAACT 
GCTGTTACAA ACAGTGCTCG CCTCATGGAA GCCATGAGTG AGGTGAAATC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis.
Nature communications712111(2016 Jul)
Banushi B,Forneris F,Straatman-Iwanowska A,Strange A,Lyne AM,Rogerson C,Burden JJ,Heywood WE,Hanley J,Doykov I,Straatman KR,Smith H,Bem D,Kriston-Vizi J,Ariceta G,Risteli M,Wang C,Ardill RE,Zaniew M,Latka-Grot J,Waddington SN,Howe SJ,Ferraro F,Gjinovci A,Lawrence S,Marsh M,Girolami M,Bozec L,Mills K,Gissen P

VPS33B regulates protein sorting into and maturation of ?-granule progenitor organelles in mouse megakaryocytes.
Blood126(2)133-43(2015 Jul)
Bem D,Smith H,Banushi B,Burden JJ,White IJ,Hanley J,Jeremiah N,Rieux-Laucat F,Bettels R,Ariceta G,Mumford AD,Thomas SG,Watson SP,Gissen P

Recruitment of VPS33A to HOPS by VPS16 Is Required for Lysosome Fusion with Endosomes and Autophagosomes.
Traffic (Copenhagen, Denmark)16(7)727-42(2015 Jul)
Wartosch L,G?nesdogan U,Graham SC,Luzio JP

Vps33b pathogenic mutations preferentially affect VIPAS39/SPE-39-positive endosomes.
Human molecular genetics22(25)5215-28(2013 Dec)
Tornieri K,Zlatic SA,Mullin AP,Werner E,Harrison R,L'hernault SW,Faundez V

Clathrin-dependent mechanisms modulate the subcellular distribution of class C Vps/HOPS tether subunits in polarized and nonpolarized cells.
Molecular biology of the cell22(10)1699-715(2011 May)
Zlatic SA,Tornieri K,L'Hernault SW,Faundez V