Pygl cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pygl
Entrez Gene ID	64035
Full Name	glycogen phosphorylase L
General protein information	Preferred Names glycogen phosphorylase L Names glycogen phosphorylase, liver form liver glycogen phosphorylase phosphorylase, glycogen, liver
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q24
Summary	catalyzes the rate-limiting step of glycogen catabolism [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022268.1	NP_071604.1	glycogen phosphorylase, liver form

Pathways from BioSystems

Homologies

Homo sapiens (human)	PYGL	NP_002854.3
Gallus gallus (chicken)	PYGL	NP_989723.1
Pan troglodytes (chimpanzee)	PYGL	XP_001145433.2
Mus musculus (house mouse)	Pygl	NP_573461.2
Saccharomyces cerevisiae (baker's yeast)	GPH1	NP_015486.1
Eremothecium gossypii	AGOS_AFR547W	NP_986094.1
Arabidopsis thaliana (thale cress)	AT3G29320	NP_189578.1
Magnaporthe oryzae (rice blast fungus)	MGG_01819	XP_003714833.1
Neurospora crassa	NCU07027	XP_962166.2
	PYGL	XP_001102253.1
Xenopus tropicalis (tropical clawed frog)	pygl	XP_002936619.1
Canis lupus familiaris (dog)	PYGL	XP_003639242.1
Bos taurus (cattle)	PYGL	NP_001068671.1
Rattus norvegicus (Norway rat)	Pygl	NP_071604.1
Kluyveromyces lactis	KLLA0F10065g	XP_455538.1

Gene Ontology
Bibliography

Related articles in PubMed

Proteomic investigation of signatures for geniposide-induced hepatotoxicity.
Wei J, Zhang F, Zhang Y, Cao C, Li X, Li D, Liu X, Yang H, Huang L
Journal of proteome research13(12)5724-33(2014 Dec)

Impact of lactobacilli on orally acquired listeriosis.
Archambaud C, Nahori MA, Soubigou G, B?cavin C, Laval L, Lechat P, Smokvina T, Langella P, Lecuit M, Cossart P
Proceedings of the National Academy of Sciences of the United States of America109(41)16684-9(2012 Oct)

Acetylation negatively regulates glycogen phosphorylase by recruiting protein phosphatase 1.
Zhang T, Wang S, Lin Y, Xu W, Ye D, Xiong Y, Zhao S, Guan KL
Cell metabolism15(1)75-87(2012 Jan)

RIP3, an energy metabolism regulator that switches TNF-induced cell death from apoptosis to necrosis.
Zhang DW, Shao J, Lin J, Zhang N, Lu BJ, Lin SC, Dong MQ, Han J
Science (New York, N.Y.)325(5938)332-6(2009 Jul)

Inhibition of the interaction between protein phosphatase 1 glycogen-targeting subunit and glycogen phosphorylase increases glycogen synthesis in primary rat hepatocytes.
Zibrova D, Grempler R, Streicher R, Kauschke SG
The Biochemical journal412(2)359-66(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that two biomarkers, glycine N-methyltransferase (GNMT) and glycogen phosphorylase (PYGL), were found to indicate early hepatic injuries.
Title: Proteomic investigation of signatures for geniposide-induced hepatotoxicity.

Data show that inhibition of the interaction between protein phosphatase 1 glycogen-targeting subunit and glycogen phosphorylase increases glycogen synthesis in primary rat hepatocytes.
Title: Inhibition of the interaction between protein phosphatase 1 glycogen-targeting subunit and glycogen phosphorylase increases glycogen synthesis in primary rat hepatocytes.

Liver-type glycogen phosphorylase can also catalyze reduction of arsenate.
Title: Glutathione-dependent reduction of arsenate by glycogen phosphorylase responsiveness to endogenous and xenobiotic inhibitors.

glycogen phosphorylase has a role in 5-phosphoribosyl-1-pyrophosphate generation in liver tissue in the basal (postabsorptive) state
Title: Modulation of glycogen phosphorylase activity affects 5-phosphoribosyl-1-pyrophosphate availability in rat hepatocyte cultures.

Results suggest that a signalling pathway involving dephosphorylation of phosphorylase a, leading to both activation and translocation of glycogen synthase, is a critical component of the mechanism by which insulin stimulates hepatic glycogen synthesis.
Title: Inactivation of phosphorylase is a major component of the mechanism by which insulin stimulates hepatic glycogen synthesis.

The following Pygl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pygl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05587	NM_022268.1 Latest version!	Rattus norvegicus glycogen phosphorylase L (Pygl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pygl ( NM_022268.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071604.1 Rattus norvegicus glycogen phosphorylase L (Pygl), mRNA.

Price & Availability↑

CloneID	ORa05587
Clone ID Related Accession (Same CDS sequence)	NM_022268.1
Accession Version	NM_022268.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	glycogen phosphorylase, liver form
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X63515.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X63515.1, BC070901.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGAAGC CCTTGACCGA CCAGGAAAAG CGACGGCAGA TCAGCATCCG CGGCATCGTG 
GGCGTGGAGA ACGTAGCGGA GCTGAAAAAG GGCTTCAATC GTCACCTGCA CTTCACTCTG 
GTCAAGGACC GCAATGTGGC CACCCCCCGC GACTACTACT TCGCCCTTGC GCACACAGTG 
CGCGACCACC TGGTGGGGCG CTGGATCCGC ACACAGCAGC ACTACTATGA CAAGTGCCCC 
AAGAGGGTGT ATTACCTCTC TCTGGAATTT TACATGGGCC GAACATTACA GAACACCATG 
ATCAACCTTG GCTTACAGAA TGCCTGCGAC GAAGCTATTT ACCAGCTCGG GCTGGACATG 
GAGGAGTTGG AAGAAATTGA AGAAGATGCT GGGCTTGGCA ATGGTGGTCT TGGGAGGCTT 
GCTGCCTGCT TCTTGGACTC CATGGCAACG CTGGGGCTTG CAGCCTATGG ATACGGCATC 
CGTTATGAAT ATGGAATCTT CAATCAGAAG ATCCGAGAAG GGTGGCAGGT AGAGGAGGCA 
GATGACTGGC TCAGGCATGG AAACCCTTGG GAGAAGGCTC GTCCTGAATT CATGCTGCCT 
GTGCATTTCT ACGGAAGAGT AGAGCACACC CAGGCAGGAA CAAAGTGGGT CGACACCCAG 
GTGGTGCTGG CTTTGCCGTA CGACACCCCC GTACCTGGGT ATATGAACAA CACGGTGAAC 
ACTATGCGCC TCTGGTCGGC CCGAGCACCC AATGACTTTA ACCTTCAAGA CTTTAATGTC 
GGAGACTACA TTCAGGCTGT GCTGGACCGG AACCTGGCTG AGAATATCTC CAGAGTGCTG 
TACCCCAACG ATAACTTTTT TGAAGGGAAG GAGCTGAGGC TGAAGCAGGA GTACTTTGTG 
GTGGCTGCGA CCCTGCAGGA TGTCATCCGA CGTTTCAAGG CCTCCAAGTT CGGCTCCAAG 
GATGGTGTAG GAACCGTGTT TGATGCTTTT CCAGATCAGG TAGCCATCCA GCTGAATGAC 
ACACATCCCG CACTCGCCAT CCCGGAGCTG ATGAGGATCT TTGTGGACAT TGAAAAATTG 
CCCTGGTCCA AGGCCTGGGA GATCACCAAG AAGACCTTTG CCTACACCAA CCACACGGTG 
CTCCCGGAGG CCCTGGAGCG CTGGCCAGTG GACCTGGTGG AGAAGCTGCT GCCTCGACAC 
TTGCAGATCA TTTATGAGAT CAATCAGAAG CATTTAGATA GAATCGTGGC CCTGTTTCCT 
AAAGACATCG ACCGCATGCG GCGGATGTCT CTCATCGAAG AGGAAGGAGG CAAAAGGATC 
AACATGGCCC ACCTCTGCAT CGTGGGCTGC CACGCGGTGA ACGGGGTAGC GAAGATCCAC 
TCGGACATCG TGAAGACCCA AGTATTCAAG GACTTCAGTG AGCTAGAACC AGACAAGTTC 
CAGAATAAAA CCAACGGGAT CACCCCGAGG CGCTGGCTCT TACTCTGCAA CCCAGGGCTG 
GCTGACTTGA TAGCAGAGAA AATTGGAGAA GACTATGTGA AAGACCTGAG CCAGCTGACG 
AAGCTCCACA GCTTCGTGGG CGACGACATC TTCCTCCGGG AAATAGCCAA AGTGAAGCAG 
GAAAATAAAC TGAAATTCTC CCAGTTCCTG GAAAAGGAGT ACAAGGTGAA GATCAACCCA 
TCTTCCATGT TTGACGTGCA CGTGAAGCGG ATCCACGAGT ACAAACGACA GCTTCTGAAC 
TGCCTGCATG TGATCACCAT GTACAATCGC ATCAAGAAAG ACCCTAAGAA GTTCTTCGTG 
CCAAGGACAG TCATAATTGG TGGGAAAGCT GCCCCAGGAT ATCACATGGC CAAAATGATC 
ATAAAGCTGG TCACCTCCGT GGCAGAAGTG GTGAACAACG ACCCTATGGT TGGCAGCAAG 
TTGAAAGTCA TCTTCTTGGA GAACTACAGA GTGTCTCTTG CTGAAAAAGT CATTCCAGCC 
ACGGACCTGT CAGAACAGAT CTCCACTGCT GGCACGGAAG CCTCGGGGAC GGGCAACATG 
AAGTTCATGC TGAACGGGGC CCTGACCATC GGGACTATGG ATGGGGCCAA TGTGGAGATG 
GCGGAGGAGG CCGGGGAGGA AAACCTGTTC ATCTTTGGCA TGAGGGTAGA TGATGTGGCC 
GCTCTGGACA AGAAAGGGTA TGAGGCCAAA GAATATTATG AGGCCCTTCC AGAACTGAAG 
CTGGTCATTG ACCAAATTGA CAATGGCTTC TTTTCTCCCA ATCAGCCAGA CCTCTTCAAA 
GACATCATCA ACATGTTATT TTATCATGAC AGATTTAAAG TCTTTGCAGA CTACGAAGCC 
TATGTCAAGT GTCAAGAAAA AGTCAGTCAG CTGTATATGA ATCAAAAAGC CTGGAACACA 
ATGGTTCTCA GAAACATAGC TGCCTCGGGG AAGTTCTCCA GTGACCGAAC AATCAGGGAG 
TATGCCAAGG ACATCTGGAA CATGGAGCCT TCCGATCTGA AGATCTCCCT ATCTAAGGAG 
TCCAGCAATG GGGTCAACGC CAATGGGAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071604.1
CDS	16..2568
Translation	MAKPLTDQEKRRQISIRGIVGVENVAELKKGFNRHLHFTLVKDRNVATPRDYYFALAHTVRDHLVGRWIRTQQHYYDKCPKRVYYLSLEFYMGRTLQNTMINLGLQNACDEAIYQLGLDMEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEYGIFNQKIREGWQVEEADDWLRHGNPWEKARPEFMLPVHFYGRVEHTQAGTKWVDTQVVLALPYDTPVPGYMNNTVNTMRLWSARAPNDFNLQDFNVGDYIQAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDVIRRFKASKFGSKDGVGTVFDAFPDQVAIQLNDTHPALAIPELMRIFVDIEKLPWSKAWEITKKTFAYTNHTVLPEALERWPVDLVEKLLPRHLQIIYEINQKHLDRIVALFPKDIDRMRRMSLIEEEGGKRINMAHLCIVGCHAVNGVAKIHSDIVKTQVFKDFSELEPDKFQNKTNGITPRRWLLLCNPGLADLIAEKIGEDYVKDLSQLTKLHSFVGDDIFLREIAKVKQENKLKFSQFLEKEYKVKINPSSMFDVHVKRIHEYKRQLLNCLHVITMYNRIKKDPKKFFVPRTVIIGGKAAPGYHMAKMIIKLVTSVAEVVNNDPMVGSKLKVIFLENYRVSLAEKVIPATDLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGEENLFIFGMRVDDVAALDKKGYEAKEYYEALPELKLVIDQIDNGFFSPNQPDLFKDIINMLFYHDRFKVFADYEAYVKCQEKVSQLYMNQKAWNTMVLRNIAASGKFSSDRTIREYAKDIWNMEPSDLKISLSKESSNGVNANGK

Target ORF information:

RefSeq Version	NM_022268.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glycogen phosphorylase L (Pygl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022268.1

ORF Insert Sequence:

ATGGCGAAGC CCTTGACCGA CCAGGAAAAG CGACGGCAGA TCAGCATCCG CGGCATCGTG 
GGCGTGGAGA ACGTAGCGGA GCTGAAAAAG GGCTTCAATC GTCACCTGCA CTTCACTCTG 
GTCAAGGACC GCAATGTGGC CACCCCCCGC GACTACTACT TCGCCCTTGC GCACACAGTG 
CGCGACCACC TGGTGGGGCG CTGGATCCGC ACACAGCAGC ACTACTATGA CAAGTGCCCC 
AAGAGGGTGT ATTACCTCTC TCTGGAATTT TACATGGGCC GAACATTACA GAACACCATG 
ATCAACCTTG GCTTACAGAA TGCCTGCGAC GAAGCTATTT ACCAGCTCGG GCTGGACATG 
GAGGAGTTGG AAGAAATTGA AGAAGATGCT GGGCTTGGCA ATGGTGGTCT TGGGAGGCTT 
GCTGCCTGCT TCTTGGACTC CATGGCAACG CTGGGGCTTG CAGCCTATGG ATACGGCATC 
CGTTATGAAT ATGGAATCTT CAATCAGAAG ATCCGAGAAG GGTGGCAGGT AGAGGAGGCA 
GATGACTGGC TCAGGCATGG AAACCCTTGG GAGAAGGCTC GTCCTGAATT CATGCTGCCT 
GTGCATTTCT ACGGAAGAGT AGAGCACACC CAGGCAGGAA CAAAGTGGGT CGACACCCAG 
GTGGTGCTGG CTTTGCCGTA CGACACCCCC GTACCTGGGT ATATGAACAA CACGGTGAAC 
ACTATGCGCC TCTGGTCGGC CCGAGCACCC AATGACTTTA ACCTTCAAGA CTTTAATGTC 
GGAGACTACA TTCAGGCTGT GCTGGACCGG AACCTGGCTG AGAATATCTC CAGAGTGCTG 
TACCCCAACG ATAACTTTTT TGAAGGGAAG GAGCTGAGGC TGAAGCAGGA GTACTTTGTG 
GTGGCTGCGA CCCTGCAGGA TGTCATCCGA CGTTTCAAGG CCTCCAAGTT CGGCTCCAAG 
GATGGTGTAG GAACCGTGTT TGATGCTTTT CCAGATCAGG TAGCCATCCA GCTGAATGAC 
ACACATCCCG CACTCGCCAT CCCGGAGCTG ATGAGGATCT TTGTGGACAT TGAAAAATTG 
CCCTGGTCCA AGGCCTGGGA GATCACCAAG AAGACCTTTG CCTACACCAA CCACACGGTG 
CTCCCGGAGG CCCTGGAGCG CTGGCCAGTG GACCTGGTGG AGAAGCTGCT GCCTCGACAC 
TTGCAGATCA TTTATGAGAT CAATCAGAAG CATTTAGATA GAATCGTGGC CCTGTTTCCT 
AAAGACATCG ACCGCATGCG GCGGATGTCT CTCATCGAAG AGGAAGGAGG CAAAAGGATC 
AACATGGCCC ACCTCTGCAT CGTGGGCTGC CACGCGGTGA ACGGGGTAGC GAAGATCCAC 
TCGGACATCG TGAAGACCCA AGTATTCAAG GACTTCAGTG AGCTAGAACC AGACAAGTTC 
CAGAATAAAA CCAACGGGAT CACCCCGAGG CGCTGGCTCT TACTCTGCAA CCCAGGGCTG 
GCTGACTTGA TAGCAGAGAA AATTGGAGAA GACTATGTGA AAGACCTGAG CCAGCTGACG 
AAGCTCCACA GCTTCGTGGG CGACGACATC TTCCTCCGGG AAATAGCCAA AGTGAAGCAG 
GAAAATAAAC TGAAATTCTC CCAGTTCCTG GAAAAGGAGT ACAAGGTGAA GATCAACCCA 
TCTTCCATGT TTGACGTGCA CGTGAAGCGG ATCCACGAGT ACAAACGACA GCTTCTGAAC 
TGCCTGCATG TGATCACCAT GTACAATCGC ATCAAGAAAG ACCCTAAGAA GTTCTTCGTG 
CCAAGGACAG TCATAATTGG TGGGAAAGCT GCCCCAGGAT ATCACATGGC CAAAATGATC 
ATAAAGCTGG TCACCTCCGT GGCAGAAGTG GTGAACAACG ACCCTATGGT TGGCAGCAAG 
TTGAAAGTCA TCTTCTTGGA GAACTACAGA GTGTCTCTTG CTGAAAAAGT CATTCCAGCC 
ACGGACCTGT CAGAACAGAT CTCCACTGCT GGCACGGAAG CCTCGGGGAC GGGCAACATG 
AAGTTCATGC TGAACGGGGC CCTGACCATC GGGACTATGG ATGGGGCCAA TGTGGAGATG 
GCGGAGGAGG CCGGGGAGGA AAACCTGTTC ATCTTTGGCA TGAGGGTAGA TGATGTGGCC 
GCTCTGGACA AGAAAGGGTA TGAGGCCAAA GAATATTATG AGGCCCTTCC AGAACTGAAG 
CTGGTCATTG ACCAAATTGA CAATGGCTTC TTTTCTCCCA ATCAGCCAGA CCTCTTCAAA 
GACATCATCA ACATGTTATT TTATCATGAC AGATTTAAAG TCTTTGCAGA CTACGAAGCC 
TATGTCAAGT GTCAAGAAAA AGTCAGTCAG CTGTATATGA ATCAAAAAGC CTGGAACACA 
ATGGTTCTCA GAAACATAGC TGCCTCGGGG AAGTTCTCCA GTGACCGAAC AATCAGGGAG 
TATGCCAAGG ACATCTGGAA CATGGAGCCT TCCGATCTGA AGATCTCCCT ATCTAAGGAG 
TCCAGCAATG GGGTCAACGC CAATGGGAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Proteomic investigation of signatures for geniposide-induced hepatotoxicity.
Journal of proteome research13(12)5724-33(2014 Dec)
Wei J,Zhang F,Zhang Y,Cao C,Li X,Li D,Liu X,Yang H,Huang L

Impact of lactobacilli on orally acquired listeriosis.
Proceedings of the National Academy of Sciences of the United States of America109(41)16684-9(2012 Oct)
Archambaud C,Nahori MA,Soubigou G,B?cavin C,Laval L,Lechat P,Smokvina T,Langella P,Lecuit M,Cossart P

Acetylation negatively regulates glycogen phosphorylase by recruiting protein phosphatase 1.
Cell metabolism15(1)75-87(2012 Jan)
Zhang T,Wang S,Lin Y,Xu W,Ye D,Xiong Y,Zhao S,Guan KL

RIP3, an energy metabolism regulator that switches TNF-induced cell death from apoptosis to necrosis.
Science (New York, N.Y.)325(5938)332-6(2009 Jul)
Zhang DW,Shao J,Lin J,Zhang N,Lu BJ,Lin SC,Dong MQ,Han J

Inhibition of the interaction between protein phosphatase 1 glycogen-targeting subunit and glycogen phosphorylase increases glycogen synthesis in primary rat hepatocytes.
The Biochemical journal412(2)359-66(2008 Jun)
Zibrova D,Grempler R,Streicher R,Kauschke SG