Syt8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Syt8
Entrez Gene ID	60566
Full Name	synaptotagmin 8
General protein information	Preferred Names synaptotagmin 8 Names synaptotagmin-8 synaptotagmin VIII sytVIII
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q41
Summary	brain-specific Ca2+/phospholipid-binding proteins [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053325.1	NP_445777.1	synaptotagmin-8

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Syt8	NP_001272790.1
Pan troglodytes (chimpanzee)	SYT8	XP_001153153.1
Canis lupus familiaris (dog)	SYT8	XP_854323.2
Gallus gallus (chicken)	SYT8	XP_004941499.1
Homo sapiens (human)	SYT8	NP_612634.3
Rattus norvegicus (Norway rat)	Syt8	NP_445777.1
Bos taurus (cattle)	SYT8	XP_003588111.2

Gene Ontology
Bibliography

Related articles in PubMed

Synaptotagmin 8 is expressed both as a calcium-insensitive soluble and membrane protein in neurons, neuroendocrine and endocrine cells.
Monterrat C, Boal F, Grise F, H?mar A, Lang J
Biochimica et biophysica acta1763(1)73-81(2006 Jan)

Synaptotagmin VI and VIII and syntaxin 2 are essential for the mouse sperm acrosome reaction.
Hutt DM, Baltz JM, Ngsee JK
The Journal of biological chemistry280(21)20197-203(2005 May)

Expression and localisation of synaptotagmin isoforms in endocrine beta-cells: their function in insulin exocytosis.
Gut A, Kiraly CE, Fukuda M, Mikoshiba K, Wollheim CB, Lang J
Journal of cell science114(Pt 9)1709-16(2001 May)

Expression of synaptotagmin VIII in rat kidney.
Kishore BK, Wade JB, Schorr K, Inoue T, Mandon B, Knepper MA
The American journal of physiology275(1 Pt 2)F131-42(1998 Jul)

Ca(2+)-dependent and -independent activities of neural and non-neural synaptotagmins.
Li C, Ullrich B, Zhang JZ, Anderson RG, Brose N, S?dhof TC
Nature375(6532)594-9(1995 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Endogenous cytosolic syt8 and transiently expressed syt8-C2AB-eGFP did not translocate upon a raise in cytosolic calcium in living cells.
Title: Synaptotagmin 8 is expressed both as a calcium-insensitive soluble and membrane protein in neurons, neuroendocrine and endocrine cells.

The following Syt8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Syt8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09281	NM_053325.1 Latest version!	Rattus norvegicus synaptotagmin 8 (Syt8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Syt8 ( NM_053325.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445777.1 Rattus norvegicus synaptotagmin 8 (Syt8), mRNA.

Price & Availability↑

CloneID	ORa09281
Clone ID Related Accession (Same CDS sequence)	NM_053325.1
Accession Version	NM_053325.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Rattus norvegicus(Norway rat)
Product	synaptotagmin-8
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF375462.1. ##Evidence-Data-START## Transcript exon combination :: AF375462.1, AF375467.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCAAGCTG ATAGGAGCAT GAAGATGGGG CATGTCTCAA ACCCCCTCAG CACCTCTGCC 
CCAGTTGATG CCACCGCTGG GCCCAATCTC ATTCCAGACC TCATTACCAA GATCCCCTGG 
CCTCGCTGGA TACTCTTTAT TGCCATCCTT GCTGCTGGAG TCCTCTTAGT CTCCTGTCTG 
CTCTGTGTCA TCTGTTACTG TTGCCACCGC CAACGCCACA GGAAGCAACC CAAGGATAAA 
GAGACTGTGG GCCTGGGCAG TGCTCGGAAC AGCACCACTA CTCACCTGGT TCAACCAGAT 
GTGGAGTGCC TAGAGCCCTG CTCTGGAGGG GACCAACCGT GGGGGCAACT GCTGCTCTCC 
CTGGAGTATG ATTTTGGAAG CCAGGAGATT AGAGTGGGCC TGAGGCAGGC TAAAAATCTG 
AAGGCTGAGG GCACAGCAGA CCCCTACGCC CGTGTCAGTG TTTCCACCCA GGCCGGGAGA 
AGACATGAGA CAAAAGTGCA CCGTGGGACA CTCTGTCCCA TGTTTGAAGA GACATGCTGC 
TTCCTGGTCC CACCAGCAGA GCTGCCCAAA GCCACTCTGA AGGTGCAGCT ATTGGACTTC 
AAGCGGTTTT CAGAACATGA GCCACTGGGG GAGCTCCAGC TACCCCTGGG CACTGTGGAC 
CTTCAGCATG TCCTGGAGAG CTGGTACCAG CTAGGCCCTC CAGGTTCCAC TGAGTCTGAG 
CAGATGGGGG AGCTGTGCTT CTCACTACGC TATGTGCCCA GCTCAGGCCG CCTGACTGTG 
GTCATACTGG AGGCTCGGGG CCTGAATCCG GGGCTTGCAG AGGCCTATGT GAAGGTCCAG 
CTTATATTAA ACCAGAGAAA GTGGAAGAAG AACAAAACAT CCTCCAAGAA GGGCACAACT 
AACCCCTACT TCAACGAGGC CTTCGTCTTC CTGGTTCCCG TTAGCCAGCT CCAGAGCGTG 
GACCTGGTGC TGGCTGTCTG GGCCCGGGGC CTGCAGCTCC TGGCTGAGCC TGTGGGCAAA 
GTGTTGCTGG GTCCCCGGGC TTCAGGCCAA CCCCTACAGC ACTGGGCAGA CATGCTGGCC 
CATGCTCGGC GGCCCATCGC CCAGTGGCAC CACCTGCGGT CACCCAGGGA AGTGGATCGT 
GCTCTGGCCC TGCAGCCTCG TCTTCCCCTG CTTAGGCCTC GCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445777.1
CDS	1..1188
Translation	MQADRSMKMGHVSNPLSTSAPVDATAGPNLIPDLITKIPWPRWILFIAILAAGVLLVSCLLCVICYCCHRQRHRKQPKDKETVGLGSARNSTTTHLVQPDVECLEPCSGGDQPWGQLLLSLEYDFGSQEIRVGLRQAKNLKAEGTADPYARVSVSTQAGRRHETKVHRGTLCPMFEETCCFLVPPAELPKATLKVQLLDFKRFSEHEPLGELQLPLGTVDLQHVLESWYQLGPPGSTESEQMGELCFSLRYVPSSGRLTVVILEARGLNPGLAEAYVKVQLILNQRKWKKNKTSSKKGTTNPYFNEAFVFLVPVSQLQSVDLVLAVWARGLQLLAEPVGKVLLGPRASGQPLQHWADMLAHARRPIAQWHHLRSPREVDRALALQPRLPLLRPRS

Target ORF information:

RefSeq Version	NM_053325.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus synaptotagmin 8 (Syt8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053325.1

ORF Insert Sequence:

ATGCAAGCTG ATAGGAGCAT GAAGATGGGG CATGTCTCAA ACCCCCTCAG CACCTCTGCC 
CCAGTTGATG CCACCGCTGG GCCCAATCTC ATTCCAGACC TCATTACCAA GATCCCCTGG 
CCTCGCTGGA TACTCTTTAT TGCCATCCTT GCTGCTGGAG TCCTCTTAGT CTCCTGTCTG 
CTCTGTGTCA TCTGTTACTG TTGCCACCGC CAACGCCACA GGAAGCAACC CAAGGATAAA 
GAGACTGTGG GCCTGGGCAG TGCTCGGAAC AGCACCACTA CTCACCTGGT TCAACCAGAT 
GTGGAGTGCC TAGAGCCCTG CTCTGGAGGG GACCAACCGT GGGGGCAACT GCTGCTCTCC 
CTGGAGTATG ATTTTGGAAG CCAGGAGATT AGAGTGGGCC TGAGGCAGGC TAAAAATCTG 
AAGGCTGAGG GCACAGCAGA CCCCTACGCC CGTGTCAGTG TTTCCACCCA GGCCGGGAGA 
AGACATGAGA CAAAAGTGCA CCGTGGGACA CTCTGTCCCA TGTTTGAAGA GACATGCTGC 
TTCCTGGTCC CACCAGCAGA GCTGCCCAAA GCCACTCTGA AGGTGCAGCT ATTGGACTTC 
AAGCGGTTTT CAGAACATGA GCCACTGGGG GAGCTCCAGC TACCCCTGGG CACTGTGGAC 
CTTCAGCATG TCCTGGAGAG CTGGTACCAG CTAGGCCCTC CAGGTTCCAC TGAGTCTGAG 
CAGATGGGGG AGCTGTGCTT CTCACTACGC TATGTGCCCA GCTCAGGCCG CCTGACTGTG 
GTCATACTGG AGGCTCGGGG CCTGAATCCG GGGCTTGCAG AGGCCTATGT GAAGGTCCAG 
CTTATATTAA ACCAGAGAAA GTGGAAGAAG AACAAAACAT CCTCCAAGAA GGGCACAACT 
AACCCCTACT TCAACGAGGC CTTCGTCTTC CTGGTTCCCG TTAGCCAGCT CCAGAGCGTG 
GACCTGGTGC TGGCTGTCTG GGCCCGGGGC CTGCAGCTCC TGGCTGAGCC TGTGGGCAAA 
GTGTTGCTGG GTCCCCGGGC TTCAGGCCAA CCCCTACAGC ACTGGGCAGA CATGCTGGCC 
CATGCTCGGC GGCCCATCGC CCAGTGGCAC CACCTGCGGT CACCCAGGGA AGTGGATCGT 
GCTCTGGCCC TGCAGCCTCG TCTTCCCCTG CTTAGGCCTC GCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Synaptotagmin 8 is expressed both as a calcium-insensitive soluble and membrane protein in neurons, neuroendocrine and endocrine cells.
Biochimica et biophysica acta1763(1)73-81(2006 Jan)
Monterrat C,Boal F,Grise F,H?mar A,Lang J

Synaptotagmin VI and VIII and syntaxin 2 are essential for the mouse sperm acrosome reaction.
The Journal of biological chemistry280(21)20197-203(2005 May)
Hutt DM,Baltz JM,Ngsee JK

Expression and localisation of synaptotagmin isoforms in endocrine beta-cells: their function in insulin exocytosis.
Journal of cell science114(Pt 9)1709-16(2001 May)
Gut A,Kiraly CE,Fukuda M,Mikoshiba K,Wollheim CB,Lang J

Expression of synaptotagmin VIII in rat kidney.
The American journal of physiology275(1 Pt 2)F131-42(1998 Jul)
Kishore BK,Wade JB,Schorr K,Inoue T,Mandon B,Knepper MA

Ca(2+)-dependent and -independent activities of neural and non-neural synaptotagmins.
Nature375(6532)594-9(1995 Jun)
Li C,Ullrich B,Zhang JZ,Anderson RG,Brose N,S?dhof TC