Kitlg cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kitlg
Entrez Gene ID	60427
Full Name	KIT ligand
Synonyms	Kitl,Mgf,SCF
General protein information	Preferred Names KIT ligand Names kit ligand C-kit ligand hematopoietic growth factor KL mast cell growth factor steel factor/kit ligand stem cell factor KL-1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q21
Summary	soluble growth factor that activates c-kit tyrosine kinase receptor; important for signaling in spermatogenesis; putative regulator of Leydig cell development [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_021843.4	NP_068615.1	kit ligand isoform 1 precursor
NM_021844.2	NP_068616.1	kit ligand isoform 2 precursor

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	kitlg	NP_001039061.1
Pan troglodytes (chimpanzee)	KITLG	XP_509255.2
Mus musculus (house mouse)	Kitl	NP_038626.1
Gallus gallus (chicken)	KITLG	NP_990461.1
Homo sapiens (human)	KITLG	NP_000890.1
Bos taurus (cattle)	KITLG	NP_776800.1
Rattus norvegicus (Norway rat)	Kitlg	NP_068615.1
	KITLG	XP_001101381.2
Canis lupus familiaris (dog)	KITLG	NP_001012753.1

Gene Ontology
Bibliography

Related articles in PubMed

Cadmium exposure in newborn rats ovary induces developmental disorders of primordial follicles and the differential expression of SCF/c-kit gene.
Zhang W, Wu T, Zhang C, Luo L, Xie M, Huang H
Toxicology letters28020-28(2017 Oct)

Aberrant expressions of stem cell factor/c-KIT in rat testis with varicocele.
Qin Q, Liu J, Ma Y, Wang Y, Zhang F, Gao S, Dong L
Journal of the Formosan Medical Association = Taiwan yi zhi116(7)542-548(2017 Jul)

NPs/NPRs Signaling Pathways May Be Involved in Depression-Induced Loss of Gastric ICC by Decreasing the Production of mSCF.
Lin XL, Tang XD, Cai ZX, Wang FY, Li P, Sui H, Guo HS
PloS one11(2)e0149031(2016)

Increased nuclear stiffness via FAK-ERK1/2 signaling is necessary for synthetic mechano-growth factor E peptide-induced tenocyte migration.
Zhang B, Luo Q, Chen Z, Shi Y, Ju Y, Yang L, Song G
Scientific reports618809(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

It was concluded that the exposure of cadimium on newborn rats ovaries could inhibit follicle development. SCF/c-kit system might mediate follicle development damage caused by cadmium, which is associated with DNA hypomethylation of SCF/c-kit promoter region may be worthy of further study.
Title: Cadmium exposure in newborn rats ovary induces developmental disorders of primordial follicles and the differential expression of SCF/c-kit gene.

up-regulation of the NPs/NPR-C and NPs/NPR-A, B/cGMP signaling pathways may be involved in depression-induced loss of gastric ICC by decreasing the production of mSCF
Title: NPs/NPRs Signaling Pathways May Be Involved in Depression-Induced Loss of Gastric ICC by Decreasing the Production of mSCF.

MGF-C25E promotes tenocyte migration by increasing nuclear stiffness via the FAK-ERK1/2 signaling pathway.
Title: Increased nuclear stiffness via FAK-ERK1/2 signaling is necessary for synthetic mechano-growth factor E peptide-induced tenocyte migration.

Downregulation of either c-kit or CXCR4 in bone marrow stem cells decreased engraftment of circulating bone marrow stem cells and inhibited reparative effects of transmyocardial revascularization.
Title: Transmyocardial Revascularization Enhances Bone Marrow Stem Cell Engraftment in Infarcted Hearts Through SCF-C-kit and SDF-1-CXCR4 Signaling Axes.

A 100 nM dose of E2 unbalanced the SCF/c-kit system, with a crucial impact on germ cell survival and thus male fertility.
Title: Estrogenic regulation of testicular expression of stem cell factor and c-kit: implications in germ cell survival and male fertility.

The following Kitlg gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kitlg cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05261	NM_021844.2 Latest version!	Rattus norvegicus KIT ligand (Kitlg), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa13628	NM_021843.4 Latest version!	Rattus norvegicus KIT ligand (Kitlg), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kitlg ( NM_021844.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_068616.1 Rattus norvegicus KIT ligand (Kitlg), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa05261
Clone ID Related Accession (Same CDS sequence)	NM_021844.2
Accession Version	NM_021844.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rattus norvegicus(Norway rat)
Product	kit ligand isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07056817.1. On Jul 6, 2012 this sequence version replaced NM_021844.1. Transcript Variant: This variant (2) lacks an alternate in-frame segment in the 3' coding region, compared to variant 1. The resulting protein (isoform 2) is shorter when it is compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB105879.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGAAGA CACAAACTTG GATTATCACT TGCATTTATC TTCAACTGCT CCTATTTAAT 
CCTCTCGTCA AAACTCAGGA GATCTGCAGG AATCCTGTGA CTGATAATGT AAAAGACATT 
ACAAAACTGG TGGCGAATCT TCCAAATGAC TATATGATAA CCCTCAACTA TGTCGCCGGG 
ATGGATGTTT TGCCTAGTCA TTGTTGGTTA CGAGATATGG TAACACACTT ATCAGTCAGC 
TTGACTACTC TTCTGGACAA GTTTTCAAAT ATTTCTGAAG GCTTGAGTAA TTATTCCATC 
ATAGACAAAC TTGGGAAAAT AGTGGATGAC CTCGTGGCAT GTATGGAAGA AAATGCACCT 
AAGAATGTAA AAGAATCACT GAAGAAGCCA GAAACTAGAA ACTTTACTCC TGAAGAATTC 
TTTAGTATTT TCAATAGATC CATTGATGCC TTCAAGGACT TCATGGTGGC ATCTGACACT 
AGTGATTGTG TGCTCTCTTC AACATTAGGT CCTGAGAAAG GGAAAGCCGC AAAGTCCCCT 
GAAGACCCAG GCCTACAATG GACAGCAATG GCACTGCCGG CTCTCATTTC GCTTGTAATT 
GGCTTTGCTT TTGGAGCCTT ATACTGGAAG AAGAAACAGT CAAGTCTTAC AAGGGCAGTT 
GAAAATATAC AGATTAATGA AGAGGATAAT GAGATAAGTA TGTTGCAACA GAAAGAGAGA 
GAGTTTCAAG AGGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068616.1
CDS	175..912
Translation	MKKTQTWIITCIYLQLLLFNPLVKTQEICRNPVTDNVKDITKLVANLPNDYMITLNYVAGMDVLPSHCWLRDMVTHLSVSLTTLLDKFSNISEGLSNYSIIDKLGKIVDDLVACMEENAPKNVKESLKKPETRNFTPEEFFSIFNRSIDAFKDFMVASDTSDCVLSSTLGPEKGKAAKSPEDPGLQWTAMALPALISLVIGFAFGALYWKKKQSSLTRAVENIQINEEDNEISMLQQKEREFQEV

Target ORF information:

RefSeq Version	NM_021844.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus KIT ligand (Kitlg), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021844.2

ORF Insert Sequence:

ATGAAGAAGA CACAAACTTG GATTATCACT TGCATTTATC TTCAACTGCT CCTATTTAAT 
CCTCTCGTCA AAACTCAGGA GATCTGCAGG AATCCTGTGA CTGATAATGT AAAAGACATT 
ACAAAACTGG TGGCGAATCT TCCAAATGAC TATATGATAA CCCTCAACTA TGTCGCCGGG 
ATGGATGTTT TGCCTAGTCA TTGTTGGTTA CGAGATATGG TAACACACTT ATCAGTCAGC 
TTGACTACTC TTCTGGACAA GTTTTCAAAT ATTTCTGAAG GCTTGAGTAA TTATTCCATC 
ATAGACAAAC TTGGGAAAAT AGTGGATGAC CTCGTGGCAT GTATGGAAGA AAATGCACCT 
AAGAATGTAA AAGAATCACT GAAGAAGCCA GAAACTAGAA ACTTTACTCC TGAAGAATTC 
TTTAGTATTT TCAATAGATC CATTGATGCC TTCAAGGACT TCATGGTGGC ATCTGACACT 
AGTGATTGTG TGCTCTCTTC AACATTAGGT CCTGAGAAAG GGAAAGCCGC AAAGTCCCCT 
GAAGACCCAG GCCTACAATG GACAGCAATG GCACTGCCGG CTCTCATTTC GCTTGTAATT 
GGCTTTGCTT TTGGAGCCTT ATACTGGAAG AAGAAACAGT CAAGTCTTAC AAGGGCAGTT 
GAAAATATAC AGATTAATGA AGAGGATAAT GAGATAAGTA TGTTGCAACA GAAAGAGAGA 
GAGTTTCAAG AGGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kitlg ( NM_021843.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_068615.1 Rattus norvegicus KIT ligand (Kitlg), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa13628
Clone ID Related Accession (Same CDS sequence)	NM_021843.4
Accession Version	NM_021843.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	kit ligand isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07056817.1. On Jul 7, 2012 this sequence version replaced NM_021843.3. Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGAAGA CACAAACTTG GATTATCACT TGCATTTATC TTCAACTGCT CCTATTTAAT 
CCTCTCGTCA AAACTCAGGA GATCTGCAGG AATCCTGTGA CTGATAATGT AAAAGACATT 
ACAAAACTGG TGGCGAATCT TCCAAATGAC TATATGATAA CCCTCAACTA TGTCGCCGGG 
ATGGATGTTT TGCCTAGTCA TTGTTGGTTA CGAGATATGG TAACACACTT ATCAGTCAGC 
TTGACTACTC TTCTGGACAA GTTTTCAAAT ATTTCTGAAG GCTTGAGTAA TTATTCCATC 
ATAGACAAAC TTGGGAAAAT AGTGGATGAC CTCGTGGCAT GTATGGAAGA AAATGCACCT 
AAGAATGTAA AAGAATCACT GAAGAAGCCA GAAACTAGAA ACTTTACTCC TGAAGAATTC 
TTTAGTATTT TCAATAGATC CATTGATGCC TTCAAGGACT TCATGGTGGC ATCTGACACT 
AGTGATTGTG TGCTCTCTTC AACATTAGGT CCTGAGAAAG ATTCCAGAGT CAGTGTCACA 
AAACCATTTA TGTTACCCCC TGTTGCAGCC AGTTCCCTTA GGAATGACAG CAGTAGCAGT 
AATAGGAAAG CCGCAAAGTC CCCTGAAGAC CCAGGCCTAC AATGGACAGC AATGGCACTG 
CCGGCTCTCA TTTCGCTTGT AATTGGCTTT GCTTTTGGAG CCTTATACTG GAAGAAGAAA 
CAGTCAAGTC TTACAAGGGC AGTTGAAAAT ATACAGATTA ATGAAGAGGA TAATGAGATA 
AGTATGTTGC AACAGAAAGA GAGAGAGTTT CAAGAGGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068615.1
CDS	175..996
Translation	MKKTQTWIITCIYLQLLLFNPLVKTQEICRNPVTDNVKDITKLVANLPNDYMITLNYVAGMDVLPSHCWLRDMVTHLSVSLTTLLDKFSNISEGLSNYSIIDKLGKIVDDLVACMEENAPKNVKESLKKPETRNFTPEEFFSIFNRSIDAFKDFMVASDTSDCVLSSTLGPEKDSRVSVTKPFMLPPVAASSLRNDSSSSNRKAAKSPEDPGLQWTAMALPALISLVIGFAFGALYWKKKQSSLTRAVENIQINEEDNEISMLQQKEREFQEV

Target ORF information:

RefSeq Version	NM_021843.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus KIT ligand (Kitlg), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021843.4

ORF Insert Sequence:

ATGAAGAAGA CACAAACTTG GATTATCACT TGCATTTATC TTCAACTGCT CCTATTTAAT 
CCTCTCGTCA AAACTCAGGA GATCTGCAGG AATCCTGTGA CTGATAATGT AAAAGACATT 
ACAAAACTGG TGGCGAATCT TCCAAATGAC TATATGATAA CCCTCAACTA TGTCGCCGGG 
ATGGATGTTT TGCCTAGTCA TTGTTGGTTA CGAGATATGG TAACACACTT ATCAGTCAGC 
TTGACTACTC TTCTGGACAA GTTTTCAAAT ATTTCTGAAG GCTTGAGTAA TTATTCCATC 
ATAGACAAAC TTGGGAAAAT AGTGGATGAC CTCGTGGCAT GTATGGAAGA AAATGCACCT 
AAGAATGTAA AAGAATCACT GAAGAAGCCA GAAACTAGAA ACTTTACTCC TGAAGAATTC 
TTTAGTATTT TCAATAGATC CATTGATGCC TTCAAGGACT TCATGGTGGC ATCTGACACT 
AGTGATTGTG TGCTCTCTTC AACATTAGGT CCTGAGAAAG ATTCCAGAGT CAGTGTCACA 
AAACCATTTA TGTTACCCCC TGTTGCAGCC AGTTCCCTTA GGAATGACAG CAGTAGCAGT 
AATAGGAAAG CCGCAAAGTC CCCTGAAGAC CCAGGCCTAC AATGGACAGC AATGGCACTG 
CCGGCTCTCA TTTCGCTTGT AATTGGCTTT GCTTTTGGAG CCTTATACTG GAAGAAGAAA 
CAGTCAAGTC TTACAAGGGC AGTTGAAAAT ATACAGATTA ATGAAGAGGA TAATGAGATA 
AGTATGTTGC AACAGAAAGA GAGAGAGTTT CAAGAGGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cadmium exposure in newborn rats ovary induces developmental disorders of primordial follicles and the differential expression of SCF/c-kit gene.
Toxicology letters28020-28(2017 Oct)
Zhang W,Wu T,Zhang C,Luo L,Xie M,Huang H

Aberrant expressions of stem cell factor/c-KIT in rat testis with varicocele.
Journal of the Formosan Medical Association = Taiwan yi zhi116(7)542-548(2017 Jul)
Qin Q,Liu J,Ma Y,Wang Y,Zhang F,Gao S,Dong L

NPs/NPRs Signaling Pathways May Be Involved in Depression-Induced Loss of Gastric ICC by Decreasing the Production of mSCF.
PloS one11(2)e0149031(2016)
Lin XL,Tang XD,Cai ZX,Wang FY,Li P,Sui H,Guo HS

Increased nuclear stiffness via FAK-ERK1/2 signaling is necessary for synthetic mechano-growth factor E peptide-induced tenocyte migration.
Scientific reports618809(2016 Jan)
Zhang B,Luo Q,Chen Z,Shi Y,Ju Y,Yang L,Song G