Slc18a3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc18a3
Entrez Gene ID	60422
Full Name	solute carrier family 18 member A3
Synonyms	VACht,rVAT
General protein information	Preferred Names solute carrier family 18 member A3 Names vesicular acetylcholine transporter solute carrier family 18 (vesicular acetylcholine transporter), member 3 solute carrier family 18 (vesicular acetylcholine), member 3 solute carrier family 18 (vesicular monoamine) member 3 solute carrier family 18, member 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16p16
Summary	a vesicular acetylcholine transporter involved in concentrating acetylcholine into synaptic vesicles [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031663.2	NP_113851.1	vesicular acetylcholine transporter

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SLC18A3	XP_001138996.1
Rattus norvegicus (Norway rat)	Slc18a3	NP_113851.1
Danio rerio (zebrafish)	slc18a3b	NP_957401.1
Caenorhabditis elegans (roundworm)	unc-17	NP_001023602.1
Xenopus tropicalis (tropical clawed frog)	slc18a3	NP_001072859.1
	SLC18A3	XP_001107292.1
Bos taurus (cattle)	SLC18A3	XP_002699016.1
Gallus gallus (chicken)	SLC18A3	NP_996865.1
Danio rerio (zebrafish)	slc18a3a	NP_001071018.1
Homo sapiens (human)	SLC18A3	NP_003046.2
Canis lupus familiaris (dog)	SLC18A3	XP_543901.2
Mus musculus (house mouse)	Slc18a3	NP_068358.2
Drosophila melanogaster (fruit fly)	VAChT	NP_477138.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002369	XP_312587.5

Gene Ontology
Bibliography

Related articles in PubMed

Acetyl-CoA the key factor for survival or death of cholinergic neurons in course of neurodegenerative diseases.
Szutowicz A, Bielarczyk H, Jankowska-Kulawy A, Pawe?czyk T, Ronowska A
Neurochemical research38(8)1523-42(2013 Aug)

Increased non-quantal release of acetylcholine after inhibition of endocytosis by methyl-?-cyclodextrin: the role of vesicular acetylcholine transporter.
Petrov AM, Naumenko NV, Uzinskaya KV, Giniatullin AR, Urazaev AK, Zefirov AL
Neuroscience1861-12(2011 Jul)

Cholinergic chemosensory cells in the trachea regulate breathing.
Krasteva G, Canning BJ, Hartmann P, Veres TZ, Papadakis T, M?hlfeld C, Schliecker K, Tallini YN, Braun A, Hackstein H, Baal N, Weihe E, Sch?tz B, Kotlikoff M, Ibanez-Tallon I, Kummer W
Proceedings of the National Academy of Sciences of the United States of America108(23)9478-83(2011 Jun)

Transcriptomic effects of depleted uranium on acetylcholine and cholesterol metabolisms in Alzheimer's disease model.
Lestaevel P, Bensoussan H, Racine R, Airault F, Gourmelon P, Souidi M
Comptes rendus biologies334(2)85-90(2011 Feb)

Segregation of met-enkephalin from vesicular acetylcholine transporter and choline acetyltransferase in sympathetic preganglionic varicosities mostly lacking synaptophysin and synaptotagmin.
S?mano C, Zetina ME, Cifuentes F, Morales MA
Neuroscience163(1)180-9(2009 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

VACHT is inserted into the presynaptic membrane during synaptic vesicles' exocytosis, removed during their endocytotic recycling, and plays a role in non-quantal secretion of acetylcholine.
Title: Increased non-quantal release of acetylcholine after inhibition of endocytosis by methyl-?-cyclodextrin: the role of vesicular acetylcholine transporter.

Sympathetic preganlionics segregate mENK from VAChT and ChAT; most of the subset of mENKergic varicosities negative for VAChT also do not express Syn, suggesting a different vesicular pattern in these sympathetic preganglionic varicosities.
Title: Segregation of met-enkephalin from vesicular acetylcholine transporter and choline acetyltransferase in sympathetic preganglionic varicosities mostly lacking synaptophysin and synaptotagmin.

results suggest that in rodent prefrontal cortex, the alpha7nAChR plays a major role in modulation of the postsynaptic excitation in spiny dendrites in contact with vesicular acetylcholine transporter containing axons
Title: Spatial and intracellular relationships between the alpha7 nicotinic acetylcholine receptor and the vesicular acetylcholine transporter in the prefrontal cortex of rat and mouse.

VAChT concentrations were increased in the brain of control SHR compared to age-matched normotensive Wistar-Kyoto rats.
Title: Vesicular acetylcholine transporter (VAChT) in the brain of spontaneously hypertensive rats (SHR): effect of treatment with an acetylcholinesterase inhibitor.

These results made [125I]-( R,R)-5-AOIBV inferior to [125I]-IBVM for the VAChT exploration in vivo.
Title: (E)-[125I]-5-AOIBV: a SPECT radioligand for the vesicular acetylcholine transporter.

The following Slc18a3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc18a3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42659	NM_031663.2 Latest version!	Rattus norvegicus solute carrier family 18 member A3 (Slc18a3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$314.30 $449.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc18a3 ( NM_031663.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113851.1 Rattus norvegicus solute carrier family 18 member A3 (Slc18a3), mRNA.

Price & Availability↑

CloneID	ORa42659
Clone ID Related Accession (Same CDS sequence)	NM_031663.2
Accession Version	NM_031663.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Rattus norvegicus(Norway rat)
Product	vesicular acetylcholine transporter
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U09211.1, U09838.1 and CB693073.1. On Nov 23, 2005 this sequence version replaced NM_031663.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: U09211.1 [ECO:0000345] ##Evidence-Data-END##

ATGGAACCCA CCGCGCCAAC CGGTCAGGCC CGGGCGGCGG CCACCAAACT GTCGGAAGCG 
GTGGGAGCCG CGCTACAAGA GCCCCAGAGG CAGCGGCGCC TGGTGCTGGT CATCGTGTGC 
GTTGCACTGT TACTGGACAA CATGTTGTAC ATGGTCATCG TGCCCATTGT TCCCGACTAT 
ATCGCCCACA TGCGCGGGGG CAGCGAGGGC CCGACCCTGG TCTCTGAGGT GTGGGAACCC 
ACTCTGCCGC CGCCCACTCT GGCTAATGCC AGTGCCTACT TGGCCAACAC GTCGGCGTCC 
CCGACGGCTG CCGGGTCGGC TCGGTCAATC CTGCGACCTC GCTACCCCAC AGAAAGCGAA 
GATGTGAAGA TAGGTGTGCT GTTTGCCTCC AAGGCTATCC TGCAGCTTCT GGTGAACCCC 
TTAAGCGGGC CTTTCATTGA TCGCATGAGC TACGACGTGC CGCTGCTTAT AGGCCTGGGC 
GTCATGTTCG CCTCCACAGT CATGTTTGCC TTTGCAGAAG ACTATGCCAC GCTCTTCGCT 
GCGCGCAGTC TACAAGGCCT GGGCTCGGCC TTCGCGGACA CGTCTGGCAT TGCCATGATC 
GCCGACAAGT ATCCCGAGGA GCCTGAGCGC AGTCGTGCCC TGGGCGTGGC GCTAGCCTTT 
ATTAGCTTTG GAAGCCTAGT GGCGCCACCG TTTGGGGGCA TCCTCTACGA GTTCGCGGGC 
AAGCGTGTAC CCTTTCTAGT GCTCGCCGCT GTGTCCCTTT TCGACGCGCT CCTGCTCCTG 
GCGGTGGCTA AGCCCTTCTC GGCTGCGGCT CGGGCGCGAG CCAACCTGCC GGTGGGCACA 
CCTATCCATC GCCTCATGCT AGACCCTTAC ATCGCTGTGG TAGCCGGCGC GCTCACCACT 
TGTAACATTC CCCTTGCGTT CCTCGAGCCC ACCATAGCCA CGTGGATGAA GCACACAATG 
GCCGCATCCG AGTGGGAGAT GGGCATGGTT TGGCTGCCGG CTTTCGTGCC ACACGTGTTA 
GGCGTCTACC TCACCGTGCG CCTGGCGGCG CGTTATCCAC ACCTGCAGTG GCTGTACGGC 
GCTCTCGGGC TAGCGGTAAT TGGAGTGAGC TCTTGCGTCG TACCTGCCTG TCGCTCATTC 
GCGCCGTTAG TGGTCTCGCT CTGCGGACTC TGCTTCGGCA TCGCGTTAGT GGACACAGCG 
CTCCTACCCA CGCTCGCCTT TCTGGTGGAC GTGCGCCACG TATCCGTCTA TGGCAGTGTC 
TATGCCATAG CTGACATCTC CTATTCTGTG GCCTACGCGC TCGGGCCCAT AGTGGCAGGC 
CACATCGTTC ACTCTCTTGG CTTTGAGCAG CTCAGCCTGG GCATGGGCCT GGCCAACCTG 
CTCTACGCAC CAGTCCTTCT TCTTTTGCGC AATGTAGGCC TCCTTACACG CTCGCGTTCG 
GAGCGCGATG TGTTGCTTGA TGAACCGCCG CAGGGTCTGT ACGACGCGGT GCGCCTGCGT 
GAGGTGCAGG GCAAGGATGG CGGCGAACCT TGTAGCCCAC CTGGCCCTTT TGACGGGTGC 
GAGGACGACT ACAACTATTA CTCCCGCAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113851.1
CDS	859..2451
Translation	MEPTAPTGQARAAATKLSEAVGAALQEPQRQRRLVLVIVCVALLLDNMLYMVIVPIVPDYIAHMRGGSEGPTLVSEVWEPTLPPPTLANASAYLANTSASPTAAGSARSILRPRYPTESEDVKIGVLFASKAILQLLVNPLSGPFIDRMSYDVPLLIGLGVMFASTVMFAFAEDYATLFAARSLQGLGSAFADTSGIAMIADKYPEEPERSRALGVALAFISFGSLVAPPFGGILYEFAGKRVPFLVLAAVSLFDALLLLAVAKPFSAAARARANLPVGTPIHRLMLDPYIAVVAGALTTCNIPLAFLEPTIATWMKHTMAASEWEMGMVWLPAFVPHVLGVYLTVRLAARYPHLQWLYGALGLAVIGVSSCVVPACRSFAPLVVSLCGLCFGIALVDTALLPTLAFLVDVRHVSVYGSVYAIADISYSVAYALGPIVAGHIVHSLGFEQLSLGMGLANLLYAPVLLLLRNVGLLTRSRSERDVLLDEPPQGLYDAVRLREVQGKDGGEPCSPPGPFDGCEDDYNYYSRS

Target ORF information:

RefSeq Version	NM_031663.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 18 member A3 (Slc18a3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031663.2

ORF Insert Sequence:

ATGGAACCCA CCGCGCCAAC CGGTCAGGCC CGGGCGGCGG CCACCAAACT GTCGGAAGCG 
GTGGGAGCCG CGCTACAAGA GCCCCAGAGG CAGCGGCGCC TGGTGCTGGT CATCGTGTGC 
GTTGCACTGT TACTGGACAA CATGTTGTAC ATGGTCATCG TGCCCATTGT TCCCGACTAT 
ATCGCCCACA TGCGCGGGGG CAGCGAGGGC CCGACCCTGG TCTCTGAGGT GTGGGAACCC 
ACTCTGCCGC CGCCCACTCT GGCTAATGCC AGTGCCTACT TGGCCAACAC GTCGGCGTCC 
CCGACGGCTG CCGGGTCGGC TCGGTCAATC CTGCGACCTC GCTACCCCAC AGAAAGCGAA 
GATGTGAAGA TAGGTGTGCT GTTTGCCTCC AAGGCTATCC TGCAGCTTCT GGTGAACCCC 
TTAAGCGGGC CTTTCATTGA TCGCATGAGC TACGACGTGC CGCTGCTTAT AGGCCTGGGC 
GTCATGTTCG CCTCCACAGT CATGTTTGCC TTTGCAGAAG ACTATGCCAC GCTCTTCGCT 
GCGCGCAGTC TACAAGGCCT GGGCTCGGCC TTCGCGGACA CGTCTGGCAT TGCCATGATC 
GCCGACAAGT ATCCCGAGGA GCCTGAGCGC AGTCGTGCCC TGGGCGTGGC GCTAGCCTTT 
ATTAGCTTTG GAAGCCTAGT GGCGCCACCG TTTGGGGGCA TCCTCTACGA GTTCGCGGGC 
AAGCGTGTAC CCTTTCTAGT GCTCGCCGCT GTGTCCCTTT TCGACGCGCT CCTGCTCCTG 
GCGGTGGCTA AGCCCTTCTC GGCTGCGGCT CGGGCGCGAG CCAACCTGCC GGTGGGCACA 
CCTATCCATC GCCTCATGCT AGACCCTTAC ATCGCTGTGG TAGCCGGCGC GCTCACCACT 
TGTAACATTC CCCTTGCGTT CCTCGAGCCC ACCATAGCCA CGTGGATGAA GCACACAATG 
GCCGCATCCG AGTGGGAGAT GGGCATGGTT TGGCTGCCGG CTTTCGTGCC ACACGTGTTA 
GGCGTCTACC TCACCGTGCG CCTGGCGGCG CGTTATCCAC ACCTGCAGTG GCTGTACGGC 
GCTCTCGGGC TAGCGGTAAT TGGAGTGAGC TCTTGCGTCG TACCTGCCTG TCGCTCATTC 
GCGCCGTTAG TGGTCTCGCT CTGCGGACTC TGCTTCGGCA TCGCGTTAGT GGACACAGCG 
CTCCTACCCA CGCTCGCCTT TCTGGTGGAC GTGCGCCACG TATCCGTCTA TGGCAGTGTC 
TATGCCATAG CTGACATCTC CTATTCTGTG GCCTACGCGC TCGGGCCCAT AGTGGCAGGC 
CACATCGTTC ACTCTCTTGG CTTTGAGCAG CTCAGCCTGG GCATGGGCCT GGCCAACCTG 
CTCTACGCAC CAGTCCTTCT TCTTTTGCGC AATGTAGGCC TCCTTACACG CTCGCGTTCG 
GAGCGCGATG TGTTGCTTGA TGAACCGCCG CAGGGTCTGT ACGACGCGGT GCGCCTGCGT 
GAGGTGCAGG GCAAGGATGG CGGCGAACCT TGTAGCCCAC CTGGCCCTTT TGACGGGTGC 
GAGGACGACT ACAACTATTA CTCCCGCAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Acetyl-CoA the key factor for survival or death of cholinergic neurons in course of neurodegenerative diseases.
Neurochemical research38(8)1523-42(2013 Aug)
Szutowicz A,Bielarczyk H,Jankowska-Kulawy A,Pawe?czyk T,Ronowska A

Increased non-quantal release of acetylcholine after inhibition of endocytosis by methyl-?-cyclodextrin: the role of vesicular acetylcholine transporter.
Neuroscience1861-12(2011 Jul)
Petrov AM,Naumenko NV,Uzinskaya KV,Giniatullin AR,Urazaev AK,Zefirov AL

Cholinergic chemosensory cells in the trachea regulate breathing.
Proceedings of the National Academy of Sciences of the United States of America108(23)9478-83(2011 Jun)
Krasteva G,Canning BJ,Hartmann P,Veres TZ,Papadakis T,M?hlfeld C,Schliecker K,Tallini YN,Braun A,Hackstein H,Baal N,Weihe E,Sch?tz B,Kotlikoff M,Ibanez-Tallon I,Kummer W

Transcriptomic effects of depleted uranium on acetylcholine and cholesterol metabolisms in Alzheimer's disease model.
Comptes rendus biologies334(2)85-90(2011 Feb)
Lestaevel P,Bensoussan H,Racine R,Airault F,Gourmelon P,Souidi M

Segregation of met-enkephalin from vesicular acetylcholine transporter and choline acetyltransferase in sympathetic preganglionic varicosities mostly lacking synaptophysin and synaptotagmin.
Neuroscience163(1)180-9(2009 Sep)
S?mano C,Zetina ME,Cifuentes F,Morales MA