Col5a3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col5a3
Entrez Gene ID	60379
Full Name	collagen type V alpha 3 chain
General protein information	Preferred Names collagen type V alpha 3 chain Names collagen alpha-3(V) chain collagen, type V, alpha 3 collagen, type V, alpha 4 procollagen, type V, alpha 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q13
Summary	alpha chain of type V collagen [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017595884.1	XP_017451373.1	collagen alpha-3(V) chain isoform X1
NM_021760.1	NP_068528.1	collagen alpha-3(V) chain precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL5A3	XP_003953413.1
Mus musculus (house mouse)	Col5a3	NP_058615.1
Bos taurus (cattle)	COL5A3	NP_001192553.1
Homo sapiens (human)	COL5A3	NP_056534.2
Rattus norvegicus (Norway rat)	Col5a3	NP_068528.1
	COL5A3	XP_002808215.1
Canis lupus familiaris (dog)	COL5A3	XP_005632967.1

Gene Ontology
Bibliography

Related articles in PubMed

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Barallobre-Barreiro J, Gupta SK, Zoccarato A, Kitazume-Taneike R, Fava M, Yin X, Werner T, Hirt MN, Zampetaki A, Viviano A, Chong M, Bern M, Kourliouros A, Domenech N, Willeit P, Shah AM, Jahangiri M, Schaefer L, Fischer JW, Iozzo RV, Viner R, Thum T, Heineke J, Kichler A, Otsu K, Mayr M
Circulation134(11)817-32(2016 Sep)

Glypican-1 and alpha4(V) collagen are required for Schwann cell myelination.
Chernousov MA, Rothblum K, Stahl RC, Evans A, Prentiss L, Carey DJ
The Journal of neuroscience : the official journal of the Society for Neuroscience26(2)508-17(2006 Jan)

Pro-alpha3(V) collagen chain is expressed in bone and its basic N-terminal peptide adheres to osteosarcoma cells.
Yamaguchi K, Matsuo N, Sumiyoshi H, Fujimoto N, Iyama KI, Yanagisawa S, Yoshioka H
Matrix biology : journal of the International Society for Matrix Biology24(4)283-94(2005 Jun)

Constitutive release of alpha4 type V collagen N-terminal domain by Schwann cells and binding to cell surface and extracellular matrix heparan sulfate proteoglycans.
Rothblum K, Stahl RC, Carey DJ
The Journal of biological chemistry279(49)51282-8(2004 Dec)

Schwann cells synthesize type V collagen that contains a novel alpha 4 chain. Molecular cloning, biochemical characterization, and high affinity heparin binding of alpha 4(V) collagen.
Chernousov MA, Rothblum K, Tyler WA, Stahl RC, Carey DJ
The Journal of biological chemistry275(36)28208-15(2000 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Alpha4(V) collagen has an important role in peripheral nerve terminal differentiation. alpha4(V) collagen coassembles with laminin on the surface of polarized Schwann cells to form tube-like ECM structures that are sites of myelination.
Title: Glypican-1 and alpha4(V) collagen are required for Schwann cell myelination.

N-terminal globular domain of the pro-alpha3(V) chain influence bone formation of osteoblasts through proteoglycan on the cell surface during development or regeneration
Title: Pro-alpha3(V) collagen chain is expressed in bone and its basic N-terminal peptide adheres to osteosarcoma cells.

alpha4(V)-NTD binding occurs via a mechanism that requires the high affinity heparin binding site and that is blocked by soluble heparin.
Title: Constitutive release of alpha4 type V collagen N-terminal domain by Schwann cells and binding to cell surface and extracellular matrix heparan sulfate proteoglycans.

The following Col5a3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col5a3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05189	NM_021760.1 Latest version!	Rattus norvegicus collagen type V alpha 3 chain (Col5a3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1532.30 $2189.00
ORa62534	XM_017595884.1 Latest version!	Rattus norvegicus collagen, type V, alpha 3 (Col5a3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col5a3 ( NM_021760.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_068528.1 Rattus norvegicus collagen type V alpha 3 chain (Col5a3), mRNA.

Price & Availability↑

CloneID	ORa05189
Clone ID Related Accession (Same CDS sequence)	NM_021760.1
Accession Version	NM_021760.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-26
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-3(V) chain precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF272661.1. ##Evidence-Data-START## Transcript exon combination :: AF272661.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAGAAGCT GCCGGAGACT GGACCAGCTT CAGGCCGGCC TCTGCCTGCT CCTGGCCTCC 
CTACAACTCG TGTCCTGGAC GCTGGCTGCA GAACCTGTGG ATGTATTGGA AACCTTGGGT 
GTGCATAGGG ACAAGGCTGG GGTGACCAAA GGGCCTGGCT TCTGCCCCCA GAGGATTCCA 
CAGGGTGACC GAGCATTCAG GGTGGGCAAG TCCAGCCTTC TCAGCGTCCC CACATGGCAG 
CTCTTTCCAG ATGGACATTT TCCTGAGAAC TTTTCTGTGC TGCTCACTCT GCGGGCCCAG 
CCAGCCAATC AATCTGTCCT TCTGTCTATA TATGACGAGA ATGGTGTCCG GCAGATGGGG 
CTGGCACTGG GGCCAGCTCT GGGTCTCCTT GGTGACTCTT TCAGGTCTCT TCCCAAGCAG 
ATCAACCTTA TGGATGGCAG GTGGCACCGG GTGGCGGTCA GCATCAGCGG TGACAAGGTG 
ACCCTGGTGG TTGACTGTGA ACCTCAGCCC CCCATGTTTG GTCAGGGGCC TCGGGTTATA 
AGTACAGCTG GACTCACGGT GATGGGAACC CAGGAGCTCG GGGAGGAGTC TTTTGAGGGA 
GACATCCAGG AGCTGCTGTT AATTCCAGAC CCTCAGGCTG CTCTCCAGGC CTGTGAGCTC 
TACCTGCCTG ACTGTGAGAC CCCCGATTCC ACAACCACAG GGGTCACTAA AGAAGAACCA 
GAAACACCTG CCCCTCGTCG ACGAAAGGGC AAAGGAAAGA AAAAAGATCG GGGTCGCAAG 
GGCAAGGGAA GAAAGAAAAA CAAGACCTCA AGACTGAGTC CAACCCCAGG TGCCCCTGAG 
AACCAGACCT CCCTCCACAC CCCTGAGACA GAGAAGCCAT CGCCCCATCT GCCTCCAACT 
CCCACACCTT TGGCCATCAC CACCACTGTC ACGATGGGAG GAAATGCCAC AGTTTTGCAG 
GGGTTGGACT CCAGTACTGA AACTGAGCAG ATGACTCCAG AGATGGACGC TACTGAAGAG 
AGTGAAGGAG GTGGCCCGAC CATGGGCCCC GAGTTCCGGG CAGCAGAACA GTCTTTACAG 
ACTGAGTTCC AGATCTTTCC TGGTGCTGGA GAAAAGGGAG CGAAAGGAGA ACCTGCGACT 
ATAGAGCAGG GACAGCAGTT TGAGGGACCT GCAGGAGCTC CAGGACCCCG GGGAATATCT 
GGTCCTTCAG GCCCTCCTGG GCCTCCGGGC TTCCCTGGGG ACCGTGGCCT ACCGGGCCCT 
GCTGGCCTCC CAGGAATCCC AGGCATTGAT GGCGTCCGGG GCCTGCCAGG CACAGTGATC 
ATGATGCCGT TCCATTTTGC AAGCGGCTCG ATGAAAGGAC CCCCAGTCTC CTTTCAACAG 
GCACAGGCCC AGGCAGTACT GCAACAGGCT CAGCTGTCCA TGAAAGGGCC CCCTGGTCCA 
GTAGGGCTCA CTGGGCGCCC AGGCCCTGTG GGCCTCCCTG GATATCCAGG TCTGAAAGGT 
GAATTGGGAG AAGCAGGGCC TCAGGGCCCC CGAGGATTGC AGGGCCCTCC TGGGCCTCCT 
GGACGGGAAG GCAAGACAGG CCGAGCTGGA GCAGATGGGG CTCGGGGTCT TCCGGGAGAC 
ACAGGACCTA AGGGTGACCG GGGCTTTGAT GGCCTGCCTG GACTGCCTGG TGAGAAGGGC 
CAGAGGGGTG ACTTTGGACC TGTAGGACAA CCTGGTCCCC CAGGAGAAGA TGGTATAAAG 
GGCCTGCAGG GACCTCCAGG CCCCACTGGC CAGGCTGGAG AGCCGGGTCC CCGAGGTCTG 
ATTGGCCCCA GAGGCCCCCC TGGTCCTTTA GGACGCCCGG GTGTGACAGG GAGTGATGGC 
GCACCGGGTG CCAAAGGCAA CGTGGGTCCT CCCGGAGAAC CAGGCCCCCC AGGACAGCAA 
GGAAACCACG GCTCCCAGGG AATCCCAGGC CCCCAGGGGC CCATTGGCAC ACCCGGGGAG 
AAGGGTCCTC CTGGAAACCC CGGAATTCCA GGTGTCCCAG GATCTGAGGG CCCCCCGGGC 
CACCCAGGCC ATGAGGGTCC CACCGGAGAA AAGGGGGCTC AGGGCCCACC AGGGTCAGCA 
GGCCCTCAGG GCTATCCTGG ACCTCGTGGT GTGAAGGGTA CCTCTGGTAA CCGGGGCCTC 
CAAGGAGAGA AAGGAGAAAG GGGAGAGGAT GGTTTTCCAG GCTTCAAGGG TGATGAGGGA 
CCAAAAGGCG ACCGGGGAAA CCCGGGACTC CCAGGTCCCA GAGGAGAGGA TGGCCCAGAA 
GGACAAAAGG GGCCTGAGGG ACTGCCTGGT GATGAGGGCC CCCCAGGAGC AGCAGGGGAG 
AAGGGCAAGC TTGGGGTGCC AGGTCTCCCA GGTTATCCAG GACGCCCAGG ACCTAAGGGA 
TCTATTGGAT TTCCTGGGCC CCTTGGACCA CTGGGGGAGA AAGGCAAGCG GGGCAAAGCA 
GGGCAGCCAG GGGAGGAAGG AGAACGTGGC ACACCGGGCA CCAGAGGAGA CAGAGGACAG 
CCAGGGGCCA CAGGCCAGCC TGGCCCTAAG GGAGATGTGG GCCAGAACGG GTCTCCTGGG 
GCCCCCGGAG AAAAGGGTCT ACCCGGTCTG CAAGGTCCCC CAGGATTCCC TGGGCCAAAA 
GGCCCCCCAG GTCCTCAGGG GAAAGATGGG ATACCCGGAC ACCCTGGACA AAGAGGAGAA 
TTGGGCTTCC AAGGTCAGAC AGGCCCCCCT GGACCAGCTG GTGTCCTTGG TCCTCAGGGA 
AAGGTAGGGG ATGTGGGTCC TCTAGGCGAG AGAGGCCCCC CAGGGCCTCC TGGACCGCCT 
GGTGAACCAG GTCTGCCAGG CATAGAAGGC AGAGAAGGGG CCAAGGGTGA GCTAGGACCC 
CTGGGGTCCC TCGGAAAGGA GGGGCCACCT GGGCCCATGG GCTTCCCTGG CCCACAAGGA 
GCCCCCGGAG ACCCAGGACC CATTGGTTTG AAGGGTGACA AGGGTCCCCC GGGCCCTCTT 
GGGGCAAATG GCTCCCCAGG TGAGCGTGGT CCTGTGGGCC CCTCTGGAGG CATTGGGCTT 
CCTGGCCAGA GTGGTGGGCA AGGCCCTATT GGTCCTGCTG GTGAGAAGGG GTCCCCGGGA 
GAACGGGGTG TGCCTGGTCC TACTGGCAAA GATGGTATTC CAGGGCCCCC GGGACTTCAG 
GGGCCCCCTG GAGCTGCAGG GCCTTCTGGT GAGGAAGGAG ACAAGGGGGA AGTAGGGATG 
CCTGGACACA AGGGAAGCAA AGGGGATAAA GGAGATGCGG GCCCACCTGG ACCAACAGGA 
ATAAGGGGTC CAGCGGGCCA TCCAGGCCCC CCGGGTGCTG ATGGTGCTCA GGGTCGCCGG 
GGACCCCCTG GCCTCTTTGG GCAGAAAGGT GATGATGGAG TCCGAGGCTT TGTAGGTGTA 
ATTGGTCCTC CAGGCCTGCA GGGGCTGCCG GGCCCTCCTG GGGAGAAAGG CGAGGTTGGA 
GACGTGGGAT CCATGGGTCC CCACGGAGCT CCAGGCCCTC GGGGTCCCCC TGGGCCCAGT 
GGATCAGCGG GTTCCCCAGG GCTGCCTGGA GGAGTAGGGC AGCCTGGCGC TGTGGGCGAG 
AAGGGTGAGC CAGGGAATGC TGGAGACGCT GGACCCCCAG GAGTTCCAGG CATCCCTGGG 
CCCAAAGGTG AAATTGGTGA AAAGGGGGAT TCGGGTCCAT CAGGGGCTGC TGGTCCTCCA 
GGCAAAAAGG GACCTCCTGG AGAGGACGGA GCTAAGGGGA ACATGGGTCC CACAGGACTC 
CCTGGAGATC TAGGACCCCC AGGAGACCCT GGAGTTCCGG GTACGGATGG CATCCCAGGG 
GAGAAGGGGA ATGCTGGTGA TATTGGGGGA CCGGGGCCAC CTGGAGCTTC AGGGGAACCT 
GGTGCCCGTG GGCTCCCTGG CAAGAGGGGT TCCCCTGGCC GCATGGGTCC AGAAGGAAAA 
GAGGGAGAGA AAGGCGCCAA GGGAGATGCT GGTCCCGATG GCCCCCCAGG CAGAACAGGC 
CCCATTGGGG CTCGGGGGCC CCCTGGACGG GTTGGGCCTG ATGGTCTTCC AGGGATCCCT 
GGTCCTGTGG GTGAACCAGG TCTCCTGGGA CCTCCTGGGC TAATCGGCCC TCCAGGGCCC 
CTGGGCCCAC CTGGCCTCCC TGGCCTGAAG GGAGACGCTG GCCTCAAGGG GGAAAAGGGC 
CACATTGGGC TAATCGGCCT CATTGGTCCC CCAGGGGAGG CTGGGGAAAA AGGCGATCAG 
GGGTTGCCAG GTGTGCAGGG CCCTCCAGGC CTTCAGGGAG ACCCTGGTCT TCCTGGTCCT 
GTTGGCTCCT TAGGTCACCC TGGGCCCCCA GGTGTGGTGG GCCCTCTGGG ACAGAAGGGT 
TCCAAGGGGT CCCCGGGATC TCTTGGACCT CGTGGAGACC CTGGACCAGC AGGTCCTCCT 
GGTCCCCCGG GTTCCCCTGC TGAGGTGCAC GGCCTGCGTA GGCGCCGCTC TGTGTTGGAC 
ACCCCAGAAG GTGGCCTGGA GGAAGTGATG GCCTCACTCA ATTCACTGAG CTTGGAGCTG 
GAGCAGTTGC AGCGACCCCC AGGCACAGCC GAGAGCCCAG GCCTCATATG CCACGAGCTT 
CATCGAAACC ACCCACACCT GCCGGACGGA GAGTACTGGA TTGACCCCAA TCAGGGCTGT 
GCACGGGACG CCTTCAAGGT CTTCTGCAAC TTCACTGCCG GAGGTGAGAC CTGTCTCTAC 
CCAGACAAGA AGTTTGAGAC GGTGAAACTG GCCTCCTGGT CCCGAGAAAA GCCTGGAGGT 
TGGTACAGCA CCTTCCGCCG AGGGAAGAAG TTCTCCTATG TGGATGCTGA CGGCTCCCCG 
GTGAATGTGG TCCAGTTGAC CTTCCTGAAG TTGTTGAGCG CTGCAGCCCA TCAGAGGTTC 
ACTTACACCT GCCAGAACTC CGCAGCATGG CTGGACGAAG CTTCAGGTGA CCACAGGCAC 
TCTATCCGCT TCCAAGGGAC CAACTGGGAA GAGTTGTCCT TCAACCAGAC AACAGCAGCC 
ACCATCAAGG TCCCCCATGA TGGCTGCCGG GTCCGGAAGG GACAGGCGAA GACCCTCTTT 
GAATTCAGCT CTTCTGTGGG CTTTCTGCCT CTGTGGGATG TGGCCGCCGT TGACTTTGGC 
CAGACGAACC AGAAGTTTGG GTTTGAACTT GGCTCCGTCT GCTTTAGCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068528.1
CDS	56..5269
Translation	MRSCRRLDQLQAGLCLLLASLQLVSWTLAAEPVDVLETLGVHRDKAGVTKGPGFCPQRIPQGDRAFRVGKSSLLSVPTWQLFPDGHFPENFSVLLTLRAQPANQSVLLSIYDENGVRQMGLALGPALGLLGDSFRSLPKQINLMDGRWHRVAVSISGDKVTLVVDCEPQPPMFGQGPRVISTAGLTVMGTQELGEESFEGDIQELLLIPDPQAALQACELYLPDCETPDSTTTGVTKEEPETPAPRRRKGKGKKKDRGRKGKGRKKNKTSRLSPTPGAPENQTSLHTPETEKPSPHLPPTPTPLAITTTVTMGGNATVLQGLDSSTETEQMTPEMDATEESEGGGPTMGPEFRAAEQSLQTEFQIFPGAGEKGAKGEPATIEQGQQFEGPAGAPGPRGISGPSGPPGPPGFPGDRGLPGPAGLPGIPGIDGVRGLPGTVIMMPFHFASGSMKGPPVSFQQAQAQAVLQQAQLSMKGPPGPVGLTGRPGPVGLPGYPGLKGELGEAGPQGPRGLQGPPGPPGREGKTGRAGADGARGLPGDTGPKGDRGFDGLPGLPGEKGQRGDFGPVGQPGPPGEDGIKGLQGPPGPTGQAGEPGPRGLIGPRGPPGPLGRPGVTGSDGAPGAKGNVGPPGEPGPPGQQGNHGSQGIPGPQGPIGTPGEKGPPGNPGIPGVPGSEGPPGHPGHEGPTGEKGAQGPPGSAGPQGYPGPRGVKGTSGNRGLQGEKGERGEDGFPGFKGDEGPKGDRGNPGLPGPRGEDGPEGQKGPEGLPGDEGPPGAAGEKGKLGVPGLPGYPGRPGPKGSIGFPGPLGPLGEKGKRGKAGQPGEEGERGTPGTRGDRGQPGATGQPGPKGDVGQNGSPGAPGEKGLPGLQGPPGFPGPKGPPGPQGKDGIPGHPGQRGELGFQGQTGPPGPAGVLGPQGKVGDVGPLGERGPPGPPGPPGEPGLPGIEGREGAKGELGPLGSLGKEGPPGPMGFPGPQGAPGDPGPIGLKGDKGPPGPLGANGSPGERGPVGPSGGIGLPGQSGGQGPIGPAGEKGSPGERGVPGPTGKDGIPGPPGLQGPPGAAGPSGEEGDKGEVGMPGHKGSKGDKGDAGPPGPTGIRGPAGHPGPPGADGAQGRRGPPGLFGQKGDDGVRGFVGVIGPPGLQGLPGPPGEKGEVGDVGSMGPHGAPGPRGPPGPSGSAGSPGLPGGVGQPGAVGEKGEPGNAGDAGPPGVPGIPGPKGEIGEKGDSGPSGAAGPPGKKGPPGEDGAKGNMGPTGLPGDLGPPGDPGVPGTDGIPGEKGNAGDIGGPGPPGASGEPGARGLPGKRGSPGRMGPEGKEGEKGAKGDAGPDGPPGRTGPIGARGPPGRVGPDGLPGIPGPVGEPGLLGPPGLIGPPGPLGPPGLPGLKGDAGLKGEKGHIGLIGLIGPPGEAGEKGDQGLPGVQGPPGLQGDPGLPGPVGSLGHPGPPGVVGPLGQKGSKGSPGSLGPRGDPGPAGPPGPPGSPAEVHGLRRRRSVLDTPEGGLEEVMASLNSLSLELEQLQRPPGTAESPGLICHELHRNHPHLPDGEYWIDPNQGCARDAFKVFCNFTAGGETCLYPDKKFETVKLASWSREKPGGWYSTFRRGKKFSYVDADGSPVNVVQLTFLKLLSAAAHQRFTYTCQNSAAWLDEASGDHRHSIRFQGTNWEELSFNQTTAATIKVPHDGCRVRKGQAKTLFEFSSSVGFLPLWDVAAVDFGQTNQKFGFELGSVCFSS

Target ORF information:

RefSeq Version	NM_021760.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen type V alpha 3 chain (Col5a3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021760.1

ORF Insert Sequence:

ATGAGAAGCT GCCGGAGACT GGACCAGCTT CAGGCCGGCC TCTGCCTGCT CCTGGCCTCC 
CTACAACTCG TGTCCTGGAC GCTGGCTGCA GAACCTGTGG ATGTATTGGA AACCTTGGGT 
GTGCATAGGG ACAAGGCTGG GGTGACCAAA GGGCCTGGCT TCTGCCCCCA GAGGATTCCA 
CAGGGTGACC GAGCATTCAG GGTGGGCAAG TCCAGCCTTC TCAGCGTCCC CACATGGCAG 
CTCTTTCCAG ATGGACATTT TCCTGAGAAC TTTTCTGTGC TGCTCACTCT GCGGGCCCAG 
CCAGCCAATC AATCTGTCCT TCTGTCTATA TATGACGAGA ATGGTGTCCG GCAGATGGGG 
CTGGCACTGG GGCCAGCTCT GGGTCTCCTT GGTGACTCTT TCAGGTCTCT TCCCAAGCAG 
ATCAACCTTA TGGATGGCAG GTGGCACCGG GTGGCGGTCA GCATCAGCGG TGACAAGGTG 
ACCCTGGTGG TTGACTGTGA ACCTCAGCCC CCCATGTTTG GTCAGGGGCC TCGGGTTATA 
AGTACAGCTG GACTCACGGT GATGGGAACC CAGGAGCTCG GGGAGGAGTC TTTTGAGGGA 
GACATCCAGG AGCTGCTGTT AATTCCAGAC CCTCAGGCTG CTCTCCAGGC CTGTGAGCTC 
TACCTGCCTG ACTGTGAGAC CCCCGATTCC ACAACCACAG GGGTCACTAA AGAAGAACCA 
GAAACACCTG CCCCTCGTCG ACGAAAGGGC AAAGGAAAGA AAAAAGATCG GGGTCGCAAG 
GGCAAGGGAA GAAAGAAAAA CAAGACCTCA AGACTGAGTC CAACCCCAGG TGCCCCTGAG 
AACCAGACCT CCCTCCACAC CCCTGAGACA GAGAAGCCAT CGCCCCATCT GCCTCCAACT 
CCCACACCTT TGGCCATCAC CACCACTGTC ACGATGGGAG GAAATGCCAC AGTTTTGCAG 
GGGTTGGACT CCAGTACTGA AACTGAGCAG ATGACTCCAG AGATGGACGC TACTGAAGAG 
AGTGAAGGAG GTGGCCCGAC CATGGGCCCC GAGTTCCGGG CAGCAGAACA GTCTTTACAG 
ACTGAGTTCC AGATCTTTCC TGGTGCTGGA GAAAAGGGAG CGAAAGGAGA ACCTGCGACT 
ATAGAGCAGG GACAGCAGTT TGAGGGACCT GCAGGAGCTC CAGGACCCCG GGGAATATCT 
GGTCCTTCAG GCCCTCCTGG GCCTCCGGGC TTCCCTGGGG ACCGTGGCCT ACCGGGCCCT 
GCTGGCCTCC CAGGAATCCC AGGCATTGAT GGCGTCCGGG GCCTGCCAGG CACAGTGATC 
ATGATGCCGT TCCATTTTGC AAGCGGCTCG ATGAAAGGAC CCCCAGTCTC CTTTCAACAG 
GCACAGGCCC AGGCAGTACT GCAACAGGCT CAGCTGTCCA TGAAAGGGCC CCCTGGTCCA 
GTAGGGCTCA CTGGGCGCCC AGGCCCTGTG GGCCTCCCTG GATATCCAGG TCTGAAAGGT 
GAATTGGGAG AAGCAGGGCC TCAGGGCCCC CGAGGATTGC AGGGCCCTCC TGGGCCTCCT 
GGACGGGAAG GCAAGACAGG CCGAGCTGGA GCAGATGGGG CTCGGGGTCT TCCGGGAGAC 
ACAGGACCTA AGGGTGACCG GGGCTTTGAT GGCCTGCCTG GACTGCCTGG TGAGAAGGGC 
CAGAGGGGTG ACTTTGGACC TGTAGGACAA CCTGGTCCCC CAGGAGAAGA TGGTATAAAG 
GGCCTGCAGG GACCTCCAGG CCCCACTGGC CAGGCTGGAG AGCCGGGTCC CCGAGGTCTG 
ATTGGCCCCA GAGGCCCCCC TGGTCCTTTA GGACGCCCGG GTGTGACAGG GAGTGATGGC 
GCACCGGGTG CCAAAGGCAA CGTGGGTCCT CCCGGAGAAC CAGGCCCCCC AGGACAGCAA 
GGAAACCACG GCTCCCAGGG AATCCCAGGC CCCCAGGGGC CCATTGGCAC ACCCGGGGAG 
AAGGGTCCTC CTGGAAACCC CGGAATTCCA GGTGTCCCAG GATCTGAGGG CCCCCCGGGC 
CACCCAGGCC ATGAGGGTCC CACCGGAGAA AAGGGGGCTC AGGGCCCACC AGGGTCAGCA 
GGCCCTCAGG GCTATCCTGG ACCTCGTGGT GTGAAGGGTA CCTCTGGTAA CCGGGGCCTC 
CAAGGAGAGA AAGGAGAAAG GGGAGAGGAT GGTTTTCCAG GCTTCAAGGG TGATGAGGGA 
CCAAAAGGCG ACCGGGGAAA CCCGGGACTC CCAGGTCCCA GAGGAGAGGA TGGCCCAGAA 
GGACAAAAGG GGCCTGAGGG ACTGCCTGGT GATGAGGGCC CCCCAGGAGC AGCAGGGGAG 
AAGGGCAAGC TTGGGGTGCC AGGTCTCCCA GGTTATCCAG GACGCCCAGG ACCTAAGGGA 
TCTATTGGAT TTCCTGGGCC CCTTGGACCA CTGGGGGAGA AAGGCAAGCG GGGCAAAGCA 
GGGCAGCCAG GGGAGGAAGG AGAACGTGGC ACACCGGGCA CCAGAGGAGA CAGAGGACAG 
CCAGGGGCCA CAGGCCAGCC TGGCCCTAAG GGAGATGTGG GCCAGAACGG GTCTCCTGGG 
GCCCCCGGAG AAAAGGGTCT ACCCGGTCTG CAAGGTCCCC CAGGATTCCC TGGGCCAAAA 
GGCCCCCCAG GTCCTCAGGG GAAAGATGGG ATACCCGGAC ACCCTGGACA AAGAGGAGAA 
TTGGGCTTCC AAGGTCAGAC AGGCCCCCCT GGACCAGCTG GTGTCCTTGG TCCTCAGGGA 
AAGGTAGGGG ATGTGGGTCC TCTAGGCGAG AGAGGCCCCC CAGGGCCTCC TGGACCGCCT 
GGTGAACCAG GTCTGCCAGG CATAGAAGGC AGAGAAGGGG CCAAGGGTGA GCTAGGACCC 
CTGGGGTCCC TCGGAAAGGA GGGGCCACCT GGGCCCATGG GCTTCCCTGG CCCACAAGGA 
GCCCCCGGAG ACCCAGGACC CATTGGTTTG AAGGGTGACA AGGGTCCCCC GGGCCCTCTT 
GGGGCAAATG GCTCCCCAGG TGAGCGTGGT CCTGTGGGCC CCTCTGGAGG CATTGGGCTT 
CCTGGCCAGA GTGGTGGGCA AGGCCCTATT GGTCCTGCTG GTGAGAAGGG GTCCCCGGGA 
GAACGGGGTG TGCCTGGTCC TACTGGCAAA GATGGTATTC CAGGGCCCCC GGGACTTCAG 
GGGCCCCCTG GAGCTGCAGG GCCTTCTGGT GAGGAAGGAG ACAAGGGGGA AGTAGGGATG 
CCTGGACACA AGGGAAGCAA AGGGGATAAA GGAGATGCGG GCCCACCTGG ACCAACAGGA 
ATAAGGGGTC CAGCGGGCCA TCCAGGCCCC CCGGGTGCTG ATGGTGCTCA GGGTCGCCGG 
GGACCCCCTG GCCTCTTTGG GCAGAAAGGT GATGATGGAG TCCGAGGCTT TGTAGGTGTA 
ATTGGTCCTC CAGGCCTGCA GGGGCTGCCG GGCCCTCCTG GGGAGAAAGG CGAGGTTGGA 
GACGTGGGAT CCATGGGTCC CCACGGAGCT CCAGGCCCTC GGGGTCCCCC TGGGCCCAGT 
GGATCAGCGG GTTCCCCAGG GCTGCCTGGA GGAGTAGGGC AGCCTGGCGC TGTGGGCGAG 
AAGGGTGAGC CAGGGAATGC TGGAGACGCT GGACCCCCAG GAGTTCCAGG CATCCCTGGG 
CCCAAAGGTG AAATTGGTGA AAAGGGGGAT TCGGGTCCAT CAGGGGCTGC TGGTCCTCCA 
GGCAAAAAGG GACCTCCTGG AGAGGACGGA GCTAAGGGGA ACATGGGTCC CACAGGACTC 
CCTGGAGATC TAGGACCCCC AGGAGACCCT GGAGTTCCGG GTACGGATGG CATCCCAGGG 
GAGAAGGGGA ATGCTGGTGA TATTGGGGGA CCGGGGCCAC CTGGAGCTTC AGGGGAACCT 
GGTGCCCGTG GGCTCCCTGG CAAGAGGGGT TCCCCTGGCC GCATGGGTCC AGAAGGAAAA 
GAGGGAGAGA AAGGCGCCAA GGGAGATGCT GGTCCCGATG GCCCCCCAGG CAGAACAGGC 
CCCATTGGGG CTCGGGGGCC CCCTGGACGG GTTGGGCCTG ATGGTCTTCC AGGGATCCCT 
GGTCCTGTGG GTGAACCAGG TCTCCTGGGA CCTCCTGGGC TAATCGGCCC TCCAGGGCCC 
CTGGGCCCAC CTGGCCTCCC TGGCCTGAAG GGAGACGCTG GCCTCAAGGG GGAAAAGGGC 
CACATTGGGC TAATCGGCCT CATTGGTCCC CCAGGGGAGG CTGGGGAAAA AGGCGATCAG 
GGGTTGCCAG GTGTGCAGGG CCCTCCAGGC CTTCAGGGAG ACCCTGGTCT TCCTGGTCCT 
GTTGGCTCCT TAGGTCACCC TGGGCCCCCA GGTGTGGTGG GCCCTCTGGG ACAGAAGGGT 
TCCAAGGGGT CCCCGGGATC TCTTGGACCT CGTGGAGACC CTGGACCAGC AGGTCCTCCT 
GGTCCCCCGG GTTCCCCTGC TGAGGTGCAC GGCCTGCGTA GGCGCCGCTC TGTGTTGGAC 
ACCCCAGAAG GTGGCCTGGA GGAAGTGATG GCCTCACTCA ATTCACTGAG CTTGGAGCTG 
GAGCAGTTGC AGCGACCCCC AGGCACAGCC GAGAGCCCAG GCCTCATATG CCACGAGCTT 
CATCGAAACC ACCCACACCT GCCGGACGGA GAGTACTGGA TTGACCCCAA TCAGGGCTGT 
GCACGGGACG CCTTCAAGGT CTTCTGCAAC TTCACTGCCG GAGGTGAGAC CTGTCTCTAC 
CCAGACAAGA AGTTTGAGAC GGTGAAACTG GCCTCCTGGT CCCGAGAAAA GCCTGGAGGT 
TGGTACAGCA CCTTCCGCCG AGGGAAGAAG TTCTCCTATG TGGATGCTGA CGGCTCCCCG 
GTGAATGTGG TCCAGTTGAC CTTCCTGAAG TTGTTGAGCG CTGCAGCCCA TCAGAGGTTC 
ACTTACACCT GCCAGAACTC CGCAGCATGG CTGGACGAAG CTTCAGGTGA CCACAGGCAC 
TCTATCCGCT TCCAAGGGAC CAACTGGGAA GAGTTGTCCT TCAACCAGAC AACAGCAGCC 
ACCATCAAGG TCCCCCATGA TGGCTGCCGG GTCCGGAAGG GACAGGCGAA GACCCTCTTT 
GAATTCAGCT CTTCTGTGGG CTTTCTGCCT CTGTGGGATG TGGCCGCCGT TGACTTTGGC 
CAGACGAACC AGAAGTTTGG GTTTGAACTT GGCTCCGTCT GCTTTAGCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col5a3 ( XM_017595884.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017451373.1 Rattus norvegicus collagen, type V, alpha 3 (Col5a3), transcript variant X1, mRNA.

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CloneID	ORa62534
Clone ID Related Accession (Same CDS sequence)	XM_017595884.1
Accession Version	XM_017595884.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-3(V) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGCT GCCGGAGACT GGACCAGCTT CAGGCCGGCC TCTGCCTGCT CCTGGCCTCC 
CTACAACTCG TGTCCTGGAC GCTGGCTGCA GAACCTGTGG ATGTATTGGA AACCTTGGGT 
GTGCATAGGG ACAAGGCTGG GGTGACCAAA GGGCCTGGCT TCTGCCCCCA GAGGATTCCA 
CAGGGTGACC GAGCATTCAG GGTGGGCAAG TCCAGCCTTC TCAGCGTCCC CACATGGCAG 
CTCTTTCCAG ATGGACATTT TCCTGAGAAC TTTTCTGTGC TGCTCACTCT GCGGGCCCAG 
CCAGCCAATC AATCTGTCCT TCTGTCTATA TATGACGAGA ATGGTGTCCG GCAGATGGGG 
CTGGCACTGG GGCCAGCTCT GGGTCTCCTT GGTGACTCTT TCAGGTCTCT TCCCAAGCAG 
ATCAACCTTA TGGATGGCAG GTGGCACCGG GTGGCGGTCA GCATCAGCGG TGACAAGGTG 
ACCCTGGTGG TTGACTGTGA ACCTCAGCCC CCCATGTTTG GTCAGGGGCC TCGGGTTATA 
AGTACAGCTG GACTCACGGT GATGGGAACC CAGGAGCTCG GGGAGGAGTC TTTTGAGGGA 
GACATCCAGG AGCTGCTGTT AATTCCAGAC CCTCAGGCTG CTCTCCAGGC CTGTGAGCTC 
TACCTGCCTG ACTGTGAGAC CCCCGATTCC ACAACCACAG GGGTCACTAA AGAAGAACCA 
GAAACACCTG CCCCTCGTCG ACGAAAGGGC AAAGGAAAGA AAAAAGATCG GGGTCGCAAG 
GGCAAGGGAA GAAAGAAAAA CAAGACCTCA AGACTGAGTC CAACCCCAGG TGCCCCTGAG 
AACCAGACCT CCCTCCACAC CCCTGAGACA GAGAAGCCAT CGCCCCATCT GCCTCCAACT 
CCCACACCTT TGGCCATCAC CACCACTGTC ACGATGGGAG GAAATGCCAC AGTTTTGCAG 
GGGTTGGACT CCAGTACTGA AACTGAGCAG ATGACTCCAG AGATGGACGC TACTGAAGAG 
AGTGAAGGAG GTGGCCCGAC CATGGGCCCC GAGTTCCGGG CAGCAGAACA GTCTTTACAG 
ACTGAGTTCC AGATCTTTCC TGGTGCTGGA GAAAAGGGAG CGAAAGGAGA ACCTGCGACT 
ATAGAGCAGG GACAGCAGTT TGAGGGACCT GCAGGAGCTC CAGGACCCCG GGGAATATCT 
GGTCCTTCAG GCCCTCCTGG GCCTCCGGGC TTCCCTGGGG ACCGTGGCCT ACCGGGCCCT 
GCTGGCCTCC CAGGAATCCC AGGCATTGAT GGCGTCCGGG GCCTGCCAGG CACAGTGATC 
ATGATGCCGT TCCATTTTGC AAGCGGCTCG ATGAAAGGAC CCCCAGTCTC CTTTCAACAG 
GCACAGGCCC AGGCAGTACT GCAACAGGCT CAGCTGTCCA TGAAAGGGCC CCCTGGTCCA 
GTAGGGCTCA CTGGGCGCCC AGGCCCTGTG GGCCTCCCTG GATATCCAGG TCTGAAAGGT 
GAATTGGGAG AAGCAGGGCC TCAGGGCCCC CGAGGATTGC AGGGCCCTCC TGGGCCTCCT 
GGACGGGAAG GCAAGACAGG CCGAGCTGGA GCAGATGGGG CTCGGGGTCT TCCGGGAGAC 
ACAGGACCTA AGGGTGACCG GGGCTTTGAT GGCCTGCCTG GACTGCCTGG TGAGAAGGGC 
CAGAGGGGTG ACTTTGGACC TGTAGGACAA CCTGGTCCCC CAGGAGAAGA TGGTATAAAG 
GGCCTGCAGG GACCTCCAGG CCCCACTGGC CAGGCTGGAG AGCCGGGTCC CCGAGGTCTG 
ATTGGCCCCA GAGGCCCCCC TGGTCCTTTA GGACGCCCGG GTGTGACAGG GAGTGATGGC 
GCACCGGGTG CCAAAGGCAA CGTGGGTCCT CCCGGAGAAC CAGGCCCCCC AGGACAGCAA 
GGAAACCACG GCTCCCAGGG AATCCCAGGC CCCCAGGGGC CCATTGGCAC ACCCGGGGAG 
AAGGGCAAGC TTGGGGTGCC AGGTCTCCCA GGTTATCCAG GACGCCCAGG ACCTAAGGGA 
TCTATTGGAT TTCCTGGGCC CCTTGGACCA CTGGGGGAGA AAGGCAAGCG GGGCAAAGCA 
GGGCAGCCAG GGGAGGAAGG AGAACGTGGC ACACCGGGCA CCAGAGGAGA CAGAGGACAG 
CCAGGGGCCA CAGGCCAGCC TGGCCCTAAG GGAGATGTGG GCCAGAACGG GTCTCCTGGG 
GCCCCCGGAG AAAAGGGTCT ACCCGGTCTG CAAGGTCCCC CAGGATTCCC TGGGCCAAAA 
GGCCCCCCAG GTCCTCAGGG GAAAGATGGG ATACCCGGAC ACCCTGGACA AAGAGGAGAA 
TTGGGCTTCC AAGGTCAGAC AGGCCCCCCT GGACCAGCTG GTGTCCTTGG TCCTCAGGGA 
AAGGTAGGGG ATGTGGGTCC TCTAGGCGAG AGAGGCCCCC CAGGGCCTCC TGGACCGCCT 
GGTGAACCAG GTCTGCCAGG CATAGAAGGC AGAGAAGGGG CCAAGGGTGA GCTAGGACCC 
CTGGGGTCCC TCGGAAAGGA GGGGCCACCT GGGCCCATGG GCTTCCCTGG CCCACAAGGA 
GCCCCCGGAG ACCCAGGACC CATTGGTTTG AAGGGTGACA AGGGTCCCCC GGGCCCTCTT 
GGGGCAAATG GCTCCCCAGG TGAGCGTGGT CCTGTGGGCC CCTCTGGAGG CATTGGGCTT 
CCTGGCCAGA GTGGTGGGCA AGGCCCTATT GGTCCTGCTG GTGAGAAGGG GTCCCCGGGA 
GAACGGGGTG TGCCTGGTCC TACTGGCAAA GATGGTATTC CAGGGCCCCC GGGACTTCAG 
GGGCCCCCTG GAGCTGCAGG GCCTTCTGGT GAGGAAGGAG ACAAGGGGGA AGTAGGGATG 
CCTGGACACA AGGGAAGCAA AGGGGATAAA GGAGATGCGG GCCCACCTGG ACCAACAGGA 
ATAAGGGGTC CAGCGGGCCA TCCAGGCCCC CCGGGTGCTG ATGGTGCTCA GGGTCGCCGG 
GGACCCCCTG GCCTCTTTGG GCAGAAAGGT GATGATGGAG TCCGAGGCTT TGTAGGTGTA 
ATTGGTCCTC CAGGCCTGCA GGGGCTGCCG GGCCCTCCTG GGGAGAAAGG CGAGGTTGGA 
GACGTGGGAT CCATGGGTCC CCACGGAGCT CCAGGCCCTC GGGGTCCCCC TGGGCCCAGT 
GGATCAGCGG GTTCCCCAGG GCTGCCTGGA GGAGTAGGGC AGCCTGGCGC TGTGGGCGAG 
AAGGGTGAGC CAGGGAATGC TGGAGACGCT GGACCCCCAG GAGTTCCAGG CATCCCTGGG 
CCCAAAGGTG AAATTGGTGA AAAGGGGGAT TCGGGTCCAT CAGGGGCTGC TGGTCCTCCA 
GGCAAAAAGG GACCTCCTGG AGAGGACGGA GCTAAGGGGA ACATGGGTCC CACAGGACTC 
CCTGGAGATC TAGGACCCCC AGGAGACCCT GGAGTTCCGG GTACGGATGG CATCCCAGGG 
GAGAAGGGGA ATGCTGGTGA TATTGGGGGA CCGGGGCCAC CTGGAGCTTC AGGGGAACCT 
GGTGCCCGTG GGCTCCCTGG CAAGAGGGGT TCCCCTGGCC GCATGGGTCC AGAAGGAAAA 
GAGGGAGAGA AAGGCGCCAA GGGAGATGCT GGTCCCGATG GCCCCCCAGG CAGAACAGGC 
CCCATTGGGG CTCGGGGGCC CCCTGGACGG GTTGGGCCTG ATGGTCTTCC AGGGATCCCT 
GGTCCTGTGG GTGAACCAGG TCTCCTGGGA CCTCCTGGGC TAATCGGCCC TCCAGGGCCC 
CTGGGCCCAC CTGGCCTCCC TGGCCTGAAG GGAGACGCTG GCCTCAAGGG GGAAAAGGGC 
CACATTGGGC TAATCGGCCT CATTGGTCCC CCAGGGGAGG CTGGGGAAAA AGGCGATCAG 
GGGTTGCCAG GTGTGCAGGG CCCTCCAGGC CTTCAGGGAG ACCCTGGTCT TCCTGGTCCT 
GTTGGCTCCT TAGGTCACCC TGGGCCCCCA GGTGTGGTGG GCCCTCTGGG ACAGAAGGGT 
TCCAAGGGGT CCCCGGGATC TCTTGGACCT CGTGGAGACC CTGGACCAGC AGGTCCTCCT 
GGTCCCCCGG GTTCCCCTGC TGAGGTGCAC GGCCTGCGTA GGCGCCGCTC TGTGTTGGAC 
ACCCCAGAAG GTGGCCTGGA GGAAGTGATG GCCTCACTCA ATTCACTGAG CTTGGAGCTG 
GAGCAGTTGC AGCGACCCCC AGGCACAGCC GAGAGCCCAG GCCTCATATG CCACGAGCTT 
CATCGAAACC ACCCACACCT GCCGGACGGA GAGTACTGGA TTGACCCCAA TCAGGGCTGT 
GCACGGGACG CCTTCAAGGT CTTCTGCAAC TTCACTGCCG GAGGTGAGAC CTGTCTCTAC 
CCAGACAAGA AGTTTGAGAC GGTGAAACTG GCCTCCTGGT CCCGAGAAAA GCCTGGAGGT 
TGGTACAGCA CCTTCCGCCG AGGGAAGAAG TTCTCCTATG TGGATGCTGA CGGCTCCCCG 
GTGAATGTGG TCCAGTTGAC CTTCCTGAAG TTGTTGAGCG CTGCAGCCCA TCAGAGGTTC 
ACTTACACCT GCCAGAACTC CGCAGCATGG CTGGACGAAG CTTCAGGTGA CCACAGGCAC 
TCTATCCGCT TCCAAGGGAC CAACTGGGAA GAGTTGTCCT TCAACCAGAC AACAGCAGCC 
ACCATCAAGG TCCCCCATGA TGGCTGCCGG GTCCGGAAGG GACAGGCGAA GACCCTCTTT 
GAATTCAGCT CTTCTGTGGG CTTTCTGCCT CTGTGGGATG TGGCCGCCGT TGACTTTGGC 
CAGACGAACC AGAAGTTTGG GTTTGAACTT GGCTCCGTCT GCTTTAGCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017451373.1
CDS	139..4992
Translation	MRSCRRLDQLQAGLCLLLASLQLVSWTLAAEPVDVLETLGVHRDKAGVTKGPGFCPQRIPQGDRAFRVGKSSLLSVPTWQLFPDGHFPENFSVLLTLRAQPANQSVLLSIYDENGVRQMGLALGPALGLLGDSFRSLPKQINLMDGRWHRVAVSISGDKVTLVVDCEPQPPMFGQGPRVISTAGLTVMGTQELGEESFEGDIQELLLIPDPQAALQACELYLPDCETPDSTTTGVTKEEPETPAPRRRKGKGKKKDRGRKGKGRKKNKTSRLSPTPGAPENQTSLHTPETEKPSPHLPPTPTPLAITTTVTMGGNATVLQGLDSSTETEQMTPEMDATEESEGGGPTMGPEFRAAEQSLQTEFQIFPGAGEKGAKGEPATIEQGQQFEGPAGAPGPRGISGPSGPPGPPGFPGDRGLPGPAGLPGIPGIDGVRGLPGTVIMMPFHFASGSMKGPPVSFQQAQAQAVLQQAQLSMKGPPGPVGLTGRPGPVGLPGYPGLKGELGEAGPQGPRGLQGPPGPPGREGKTGRAGADGARGLPGDTGPKGDRGFDGLPGLPGEKGQRGDFGPVGQPGPPGEDGIKGLQGPPGPTGQAGEPGPRGLIGPRGPPGPLGRPGVTGSDGAPGAKGNVGPPGEPGPPGQQGNHGSQGIPGPQGPIGTPGEKGKLGVPGLPGYPGRPGPKGSIGFPGPLGPLGEKGKRGKAGQPGEEGERGTPGTRGDRGQPGATGQPGPKGDVGQNGSPGAPGEKGLPGLQGPPGFPGPKGPPGPQGKDGIPGHPGQRGELGFQGQTGPPGPAGVLGPQGKVGDVGPLGERGPPGPPGPPGEPGLPGIEGREGAKGELGPLGSLGKEGPPGPMGFPGPQGAPGDPGPIGLKGDKGPPGPLGANGSPGERGPVGPSGGIGLPGQSGGQGPIGPAGEKGSPGERGVPGPTGKDGIPGPPGLQGPPGAAGPSGEEGDKGEVGMPGHKGSKGDKGDAGPPGPTGIRGPAGHPGPPGADGAQGRRGPPGLFGQKGDDGVRGFVGVIGPPGLQGLPGPPGEKGEVGDVGSMGPHGAPGPRGPPGPSGSAGSPGLPGGVGQPGAVGEKGEPGNAGDAGPPGVPGIPGPKGEIGEKGDSGPSGAAGPPGKKGPPGEDGAKGNMGPTGLPGDLGPPGDPGVPGTDGIPGEKGNAGDIGGPGPPGASGEPGARGLPGKRGSPGRMGPEGKEGEKGAKGDAGPDGPPGRTGPIGARGPPGRVGPDGLPGIPGPVGEPGLLGPPGLIGPPGPLGPPGLPGLKGDAGLKGEKGHIGLIGLIGPPGEAGEKGDQGLPGVQGPPGLQGDPGLPGPVGSLGHPGPPGVVGPLGQKGSKGSPGSLGPRGDPGPAGPPGPPGSPAEVHGLRRRRSVLDTPEGGLEEVMASLNSLSLELEQLQRPPGTAESPGLICHELHRNHPHLPDGEYWIDPNQGCARDAFKVFCNFTAGGETCLYPDKKFETVKLASWSREKPGGWYSTFRRGKKFSYVDADGSPVNVVQLTFLKLLSAAAHQRFTYTCQNSAAWLDEASGDHRHSIRFQGTNWEELSFNQTTAATIKVPHDGCRVRKGQAKTLFEFSSSVGFLPLWDVAAVDFGQTNQKFGFELGSVCFSS

Target ORF information:

RefSeq Version	XM_017595884.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen, type V, alpha 3 (Col5a3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595884.1

ORF Insert Sequence:

ATGAGAAGCT GCCGGAGACT GGACCAGCTT CAGGCCGGCC TCTGCCTGCT CCTGGCCTCC 
CTACAACTCG TGTCCTGGAC GCTGGCTGCA GAACCTGTGG ATGTATTGGA AACCTTGGGT 
GTGCATAGGG ACAAGGCTGG GGTGACCAAA GGGCCTGGCT TCTGCCCCCA GAGGATTCCA 
CAGGGTGACC GAGCATTCAG GGTGGGCAAG TCCAGCCTTC TCAGCGTCCC CACATGGCAG 
CTCTTTCCAG ATGGACATTT TCCTGAGAAC TTTTCTGTGC TGCTCACTCT GCGGGCCCAG 
CCAGCCAATC AATCTGTCCT TCTGTCTATA TATGACGAGA ATGGTGTCCG GCAGATGGGG 
CTGGCACTGG GGCCAGCTCT GGGTCTCCTT GGTGACTCTT TCAGGTCTCT TCCCAAGCAG 
ATCAACCTTA TGGATGGCAG GTGGCACCGG GTGGCGGTCA GCATCAGCGG TGACAAGGTG 
ACCCTGGTGG TTGACTGTGA ACCTCAGCCC CCCATGTTTG GTCAGGGGCC TCGGGTTATA 
AGTACAGCTG GACTCACGGT GATGGGAACC CAGGAGCTCG GGGAGGAGTC TTTTGAGGGA 
GACATCCAGG AGCTGCTGTT AATTCCAGAC CCTCAGGCTG CTCTCCAGGC CTGTGAGCTC 
TACCTGCCTG ACTGTGAGAC CCCCGATTCC ACAACCACAG GGGTCACTAA AGAAGAACCA 
GAAACACCTG CCCCTCGTCG ACGAAAGGGC AAAGGAAAGA AAAAAGATCG GGGTCGCAAG 
GGCAAGGGAA GAAAGAAAAA CAAGACCTCA AGACTGAGTC CAACCCCAGG TGCCCCTGAG 
AACCAGACCT CCCTCCACAC CCCTGAGACA GAGAAGCCAT CGCCCCATCT GCCTCCAACT 
CCCACACCTT TGGCCATCAC CACCACTGTC ACGATGGGAG GAAATGCCAC AGTTTTGCAG 
GGGTTGGACT CCAGTACTGA AACTGAGCAG ATGACTCCAG AGATGGACGC TACTGAAGAG 
AGTGAAGGAG GTGGCCCGAC CATGGGCCCC GAGTTCCGGG CAGCAGAACA GTCTTTACAG 
ACTGAGTTCC AGATCTTTCC TGGTGCTGGA GAAAAGGGAG CGAAAGGAGA ACCTGCGACT 
ATAGAGCAGG GACAGCAGTT TGAGGGACCT GCAGGAGCTC CAGGACCCCG GGGAATATCT 
GGTCCTTCAG GCCCTCCTGG GCCTCCGGGC TTCCCTGGGG ACCGTGGCCT ACCGGGCCCT 
GCTGGCCTCC CAGGAATCCC AGGCATTGAT GGCGTCCGGG GCCTGCCAGG CACAGTGATC 
ATGATGCCGT TCCATTTTGC AAGCGGCTCG ATGAAAGGAC CCCCAGTCTC CTTTCAACAG 
GCACAGGCCC AGGCAGTACT GCAACAGGCT CAGCTGTCCA TGAAAGGGCC CCCTGGTCCA 
GTAGGGCTCA CTGGGCGCCC AGGCCCTGTG GGCCTCCCTG GATATCCAGG TCTGAAAGGT 
GAATTGGGAG AAGCAGGGCC TCAGGGCCCC CGAGGATTGC AGGGCCCTCC TGGGCCTCCT 
GGACGGGAAG GCAAGACAGG CCGAGCTGGA GCAGATGGGG CTCGGGGTCT TCCGGGAGAC 
ACAGGACCTA AGGGTGACCG GGGCTTTGAT GGCCTGCCTG GACTGCCTGG TGAGAAGGGC 
CAGAGGGGTG ACTTTGGACC TGTAGGACAA CCTGGTCCCC CAGGAGAAGA TGGTATAAAG 
GGCCTGCAGG GACCTCCAGG CCCCACTGGC CAGGCTGGAG AGCCGGGTCC CCGAGGTCTG 
ATTGGCCCCA GAGGCCCCCC TGGTCCTTTA GGACGCCCGG GTGTGACAGG GAGTGATGGC 
GCACCGGGTG CCAAAGGCAA CGTGGGTCCT CCCGGAGAAC CAGGCCCCCC AGGACAGCAA 
GGAAACCACG GCTCCCAGGG AATCCCAGGC CCCCAGGGGC CCATTGGCAC ACCCGGGGAG 
AAGGGCAAGC TTGGGGTGCC AGGTCTCCCA GGTTATCCAG GACGCCCAGG ACCTAAGGGA 
TCTATTGGAT TTCCTGGGCC CCTTGGACCA CTGGGGGAGA AAGGCAAGCG GGGCAAAGCA 
GGGCAGCCAG GGGAGGAAGG AGAACGTGGC ACACCGGGCA CCAGAGGAGA CAGAGGACAG 
CCAGGGGCCA CAGGCCAGCC TGGCCCTAAG GGAGATGTGG GCCAGAACGG GTCTCCTGGG 
GCCCCCGGAG AAAAGGGTCT ACCCGGTCTG CAAGGTCCCC CAGGATTCCC TGGGCCAAAA 
GGCCCCCCAG GTCCTCAGGG GAAAGATGGG ATACCCGGAC ACCCTGGACA AAGAGGAGAA 
TTGGGCTTCC AAGGTCAGAC AGGCCCCCCT GGACCAGCTG GTGTCCTTGG TCCTCAGGGA 
AAGGTAGGGG ATGTGGGTCC TCTAGGCGAG AGAGGCCCCC CAGGGCCTCC TGGACCGCCT 
GGTGAACCAG GTCTGCCAGG CATAGAAGGC AGAGAAGGGG CCAAGGGTGA GCTAGGACCC 
CTGGGGTCCC TCGGAAAGGA GGGGCCACCT GGGCCCATGG GCTTCCCTGG CCCACAAGGA 
GCCCCCGGAG ACCCAGGACC CATTGGTTTG AAGGGTGACA AGGGTCCCCC GGGCCCTCTT 
GGGGCAAATG GCTCCCCAGG TGAGCGTGGT CCTGTGGGCC CCTCTGGAGG CATTGGGCTT 
CCTGGCCAGA GTGGTGGGCA AGGCCCTATT GGTCCTGCTG GTGAGAAGGG GTCCCCGGGA 
GAACGGGGTG TGCCTGGTCC TACTGGCAAA GATGGTATTC CAGGGCCCCC GGGACTTCAG 
GGGCCCCCTG GAGCTGCAGG GCCTTCTGGT GAGGAAGGAG ACAAGGGGGA AGTAGGGATG 
CCTGGACACA AGGGAAGCAA AGGGGATAAA GGAGATGCGG GCCCACCTGG ACCAACAGGA 
ATAAGGGGTC CAGCGGGCCA TCCAGGCCCC CCGGGTGCTG ATGGTGCTCA GGGTCGCCGG 
GGACCCCCTG GCCTCTTTGG GCAGAAAGGT GATGATGGAG TCCGAGGCTT TGTAGGTGTA 
ATTGGTCCTC CAGGCCTGCA GGGGCTGCCG GGCCCTCCTG GGGAGAAAGG CGAGGTTGGA 
GACGTGGGAT CCATGGGTCC CCACGGAGCT CCAGGCCCTC GGGGTCCCCC TGGGCCCAGT 
GGATCAGCGG GTTCCCCAGG GCTGCCTGGA GGAGTAGGGC AGCCTGGCGC TGTGGGCGAG 
AAGGGTGAGC CAGGGAATGC TGGAGACGCT GGACCCCCAG GAGTTCCAGG CATCCCTGGG 
CCCAAAGGTG AAATTGGTGA AAAGGGGGAT TCGGGTCCAT CAGGGGCTGC TGGTCCTCCA 
GGCAAAAAGG GACCTCCTGG AGAGGACGGA GCTAAGGGGA ACATGGGTCC CACAGGACTC 
CCTGGAGATC TAGGACCCCC AGGAGACCCT GGAGTTCCGG GTACGGATGG CATCCCAGGG 
GAGAAGGGGA ATGCTGGTGA TATTGGGGGA CCGGGGCCAC CTGGAGCTTC AGGGGAACCT 
GGTGCCCGTG GGCTCCCTGG CAAGAGGGGT TCCCCTGGCC GCATGGGTCC AGAAGGAAAA 
GAGGGAGAGA AAGGCGCCAA GGGAGATGCT GGTCCCGATG GCCCCCCAGG CAGAACAGGC 
CCCATTGGGG CTCGGGGGCC CCCTGGACGG GTTGGGCCTG ATGGTCTTCC AGGGATCCCT 
GGTCCTGTGG GTGAACCAGG TCTCCTGGGA CCTCCTGGGC TAATCGGCCC TCCAGGGCCC 
CTGGGCCCAC CTGGCCTCCC TGGCCTGAAG GGAGACGCTG GCCTCAAGGG GGAAAAGGGC 
CACATTGGGC TAATCGGCCT CATTGGTCCC CCAGGGGAGG CTGGGGAAAA AGGCGATCAG 
GGGTTGCCAG GTGTGCAGGG CCCTCCAGGC CTTCAGGGAG ACCCTGGTCT TCCTGGTCCT 
GTTGGCTCCT TAGGTCACCC TGGGCCCCCA GGTGTGGTGG GCCCTCTGGG ACAGAAGGGT 
TCCAAGGGGT CCCCGGGATC TCTTGGACCT CGTGGAGACC CTGGACCAGC AGGTCCTCCT 
GGTCCCCCGG GTTCCCCTGC TGAGGTGCAC GGCCTGCGTA GGCGCCGCTC TGTGTTGGAC 
ACCCCAGAAG GTGGCCTGGA GGAAGTGATG GCCTCACTCA ATTCACTGAG CTTGGAGCTG 
GAGCAGTTGC AGCGACCCCC AGGCACAGCC GAGAGCCCAG GCCTCATATG CCACGAGCTT 
CATCGAAACC ACCCACACCT GCCGGACGGA GAGTACTGGA TTGACCCCAA TCAGGGCTGT 
GCACGGGACG CCTTCAAGGT CTTCTGCAAC TTCACTGCCG GAGGTGAGAC CTGTCTCTAC 
CCAGACAAGA AGTTTGAGAC GGTGAAACTG GCCTCCTGGT CCCGAGAAAA GCCTGGAGGT 
TGGTACAGCA CCTTCCGCCG AGGGAAGAAG TTCTCCTATG TGGATGCTGA CGGCTCCCCG 
GTGAATGTGG TCCAGTTGAC CTTCCTGAAG TTGTTGAGCG CTGCAGCCCA TCAGAGGTTC 
ACTTACACCT GCCAGAACTC CGCAGCATGG CTGGACGAAG CTTCAGGTGA CCACAGGCAC 
TCTATCCGCT TCCAAGGGAC CAACTGGGAA GAGTTGTCCT TCAACCAGAC AACAGCAGCC 
ACCATCAAGG TCCCCCATGA TGGCTGCCGG GTCCGGAAGG GACAGGCGAA GACCCTCTTT 
GAATTCAGCT CTTCTGTGGG CTTTCTGCCT CTGTGGGATG TGGCCGCCGT TGACTTTGGC 
CAGACGAACC AGAAGTTTGG GTTTGAACTT GGCTCCGTCT GCTTTAGCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Circulation134(11)817-32(2016 Sep)
Barallobre-Barreiro J,Gupta SK,Zoccarato A,Kitazume-Taneike R,Fava M,Yin X,Werner T,Hirt MN,Zampetaki A,Viviano A,Chong M,Bern M,Kourliouros A,Domenech N,Willeit P,Shah AM,Jahangiri M,Schaefer L,Fischer JW,Iozzo RV,Viner R,Thum T,Heineke J,Kichler A,Otsu K,Mayr M

Glypican-1 and alpha4(V) collagen are required for Schwann cell myelination.
The Journal of neuroscience : the official journal of the Society for Neuroscience26(2)508-17(2006 Jan)
Chernousov MA,Rothblum K,Stahl RC,Evans A,Prentiss L,Carey DJ

Pro-alpha3(V) collagen chain is expressed in bone and its basic N-terminal peptide adheres to osteosarcoma cells.
Matrix biology : journal of the International Society for Matrix Biology24(4)283-94(2005 Jun)
Yamaguchi K,Matsuo N,Sumiyoshi H,Fujimoto N,Iyama KI,Yanagisawa S,Yoshioka H

Constitutive release of alpha4 type V collagen N-terminal domain by Schwann cells and binding to cell surface and extracellular matrix heparan sulfate proteoglycans.
The Journal of biological chemistry279(49)51282-8(2004 Dec)
Rothblum K,Stahl RC,Carey DJ

Schwann cells synthesize type V collagen that contains a novel alpha 4 chain. Molecular cloning, biochemical characterization, and high affinity heparin binding of alpha 4(V) collagen.
The Journal of biological chemistry275(36)28208-15(2000 Sep)
Chernousov MA,Rothblum K,Tyler WA,Stahl RC,Carey DJ