Ensa cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ensa
Entrez Gene ID	60334
Full Name	endosulfine alpha
General protein information	Preferred Names endosulfine alpha Names alpha-endosulfine ARPP-19e
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34
Summary	may mediate insulin secretion through interaction with the pancreatic beta-cell ATP-sensitive potassium (K(ATP)) channel [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_021842.3	NP_068614.1	alpha-endosulfine isoform 2
NM_001033974.2	NP_001029146.1	alpha-endosulfine isoform 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ensa	NP_062507.1
Homo sapiens (human)	ENSA	NP_996927.1
Canis lupus familiaris (dog)	ENSA	XP_860994.1
Pan troglodytes (chimpanzee)	ENSA	XP_003949584.1
Bos taurus (cattle)	ENSA	XP_005204022.1
Rattus norvegicus (Norway rat)	Ensa	NP_068614.1
	LOC715857	XP_001106271.1
Gallus gallus (chicken)	ENSA	NP_001007966.1
Danio rerio (zebrafish)	ensab	NP_001243660.1
Xenopus tropicalis (tropical clawed frog)	ensa	NP_998845.1

Gene Ontology
Bibliography

Related articles in PubMed

?-Endosulfine (ARPP-19e) Expression in a Rat Model of Stroke.
Mehta RI, Tsymbalyuk N, Ivanova S, Stokum JA, Woo K, Gerzanich V, Simard JM
Journal of neuropathology and experimental neurology76(10)898-907(2017 10)

cDNA microarray analysis of spinal cord injury and regeneration related genes in rat.
Xiao L, Ma ZL, Li X, Lin QX, Que HP, Liu SJ
Sheng li xue bao : [Acta physiologica Sinica]57(6)705-13(2005 Dec)

Rat mesangial alpha-endosulfine.
Yee J, Cortes P, Barnes JL, Alviani R, Biederman JI, Szamosfalvi B
Kidney international65(5)1731-9(2004 May)

Gene expression of alpha-endosulfine in the rat brain: correlative changes with aging, learning and stress.
Dou J, Cui C, Dufour F, Alkon DL, Zhao WQ
Journal of neurochemistry87(5)1086-100(2003 Dec)

Localization of alpha-endosulphine in pancreatic somatostatin delta cells and expression during rat pancreas development.
Gros L, Br?ant B, Duchene B, Leroy C, Fauconnier G, Bataille D, Virsolvy A
Diabetologia45(5)703-10(2002 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study demonstrated that the alphaEnsa levels are significantly upregulated in mammalian brain at various times following the onset of ischemia/reperfusion. Protein upregulation was detected as early as 2 hours after ischemic onset, with sustained upregulation noted subsequently over several days
Title: ?-Endosulfine (ARPP-19e) Expression in a Rat Model of Stroke.

ENSA and alpha-endosulfine are expressed in glomeruli and mesangial cells, and gene and protein expression are up-regulated by a high glucose environment.
Title: Rat mesangial alpha-endosulfine.

results suggest that alpha-endosulfine gene expression is differentially regulated by distinct brain processes involving excitatory neuronal activities
Title: Gene expression of alpha-endosulfine in the rat brain: correlative changes with aging, learning and stress.

localization in pancreatic somatostatin delta cells and expression during rat pancreas development
Title: Localization of alpha-endosulphine in pancreatic somatostatin delta cells and expression during rat pancreas development.

The following Ensa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ensa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08805	NM_001033974.2 Latest version!	Rattus norvegicus endosulfine alpha (Ensa), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa05254	NM_021842.3 Latest version!	Rattus norvegicus endosulfine alpha (Ensa), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ensa ( NM_001033974.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001029146.1 Rattus norvegicus endosulfine alpha (Ensa), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa08805
Clone ID Related Accession (Same CDS sequence)	NM_001033974.2
Accession Version	NM_001033974.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Rattus norvegicus(Norway rat)
Product	alpha-endosulfine isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC059135.1 and AABR07012575.1. On Nov 23, 2013 this sequence version replaced NM_001033974.1. Transcript Variant: This variant (1) represents the longer transcript and encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC059135.1, FQ216114.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTCCCAGA AACAAGAAGA AGAAAACCCT GCGGAGGAAA CCGGCGAGGA GAAGCAGGAT 
ACACAGGAGA AAGAAGGTAT TCTTCCTGAG AAAGCTGAGG AAGCAAAGCT AAAGGCCAAA 
TATCCAAGCC TAGGACAAAA GCCTGGAGGC TCCGACTTCC TCATGAAGAG ACTCCAGAAA 
GGGCAAAAGT ACTTTGACTC AGGAGACTAC AACATGGCCA AAGCCAAGAT GAAGAACAAG 
CAGCTACCGA GTGCAGGAGC AGACAAGAAC CTGGTGACCG GTGACCACAT CCCCACCCCA 
CAGGACCTGC CCCAGAGAAA GTCCTCGCTC GTCACCAGCA AGCTTGCGGG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001029146.1
CDS	141..494
Translation	MSQKQEEENPAEETGEEKQDTQEKEGILPEKAEEAKLKAKYPSLGQKPGGSDFLMKRLQKGQKYFDSGDYNMAKAKMKNKQLPSAGADKNLVTGDHIPTPQDLPQRKSSLVTSKLAG

Target ORF information:

RefSeq Version	NM_001033974.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus endosulfine alpha (Ensa), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001033974.2

ORF Insert Sequence:

ATGTCCCAGA AACAAGAAGA AGAAAACCCT GCGGAGGAAA CCGGCGAGGA GAAGCAGGAT 
ACACAGGAGA AAGAAGGTAT TCTTCCTGAG AAAGCTGAGG AAGCAAAGCT AAAGGCCAAA 
TATCCAAGCC TAGGACAAAA GCCTGGAGGC TCCGACTTCC TCATGAAGAG ACTCCAGAAA 
GGGCAAAAGT ACTTTGACTC AGGAGACTAC AACATGGCCA AAGCCAAGAT GAAGAACAAG 
CAGCTACCGA GTGCAGGAGC AGACAAGAAC CTGGTGACCG GTGACCACAT CCCCACCCCA 
CAGGACCTGC CCCAGAGAAA GTCCTCGCTC GTCACCAGCA AGCTTGCGGG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ensa ( NM_021842.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_068614.1 Rattus norvegicus endosulfine alpha (Ensa), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa05254
Clone ID Related Accession (Same CDS sequence)	NM_021842.3
Accession Version	NM_021842.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Rattus norvegicus(Norway rat)
Product	alpha-endosulfine isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07012575.1 and CB812628.1. On Nov 23, 2013 this sequence version replaced NM_021842.2. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region and a different 3' terminal exon compared to variant 1. The resulting protein (isoform 2) is longer and has a distinct C-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: FQ213912.1, FQ212892.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTCCCAGA AACAAGAAGA AGAAAACCCT GCGGAGGAAA CCGGCGAGGA GAAGCAGGAT 
ACACAGGAGA AAGAAGGTAT TCTTCCTGAG AAAGCTGAGG AAGCAAAGCT AAAGGCCAAA 
TATCCAAGCC TAGGACAAAA GCCTGGAGGC TCCGACTTCC TCATGAAGAG ACTCCAGAAA 
GGGCAAAAGT ACTTTGACTC AGGAGACTAC AACATGGCCA AAGCCAAGAT GAAGAACAAG 
CAGCTACCGA GTGCAGGAGC AGACAAGAAC CTGGTGACCG GTGACCACAT CCCCACCCCA 
CAGGACCTGC CCCAGAGAAA GTCCTCGCTC GTCACCAGCA AGCTTGCGGG TGGCCAAGTT 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068614.1
CDS	141..506
Translation	MSQKQEEENPAEETGEEKQDTQEKEGILPEKAEEAKLKAKYPSLGQKPGGSDFLMKRLQKGQKYFDSGDYNMAKAKMKNKQLPSAGADKNLVTGDHIPTPQDLPQRKSSLVTSKLAGGQVE

Target ORF information:

RefSeq Version	NM_021842.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus endosulfine alpha (Ensa), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021842.3

ORF Insert Sequence:

ATGTCCCAGA AACAAGAAGA AGAAAACCCT GCGGAGGAAA CCGGCGAGGA GAAGCAGGAT 
ACACAGGAGA AAGAAGGTAT TCTTCCTGAG AAAGCTGAGG AAGCAAAGCT AAAGGCCAAA 
TATCCAAGCC TAGGACAAAA GCCTGGAGGC TCCGACTTCC TCATGAAGAG ACTCCAGAAA 
GGGCAAAAGT ACTTTGACTC AGGAGACTAC AACATGGCCA AAGCCAAGAT GAAGAACAAG 
CAGCTACCGA GTGCAGGAGC AGACAAGAAC CTGGTGACCG GTGACCACAT CCCCACCCCA 
CAGGACCTGC CCCAGAGAAA GTCCTCGCTC GTCACCAGCA AGCTTGCGGG TGGCCAAGTT 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

?-Endosulfine (ARPP-19e) Expression in a Rat Model of Stroke.
Journal of neuropathology and experimental neurology76(10)898-907(2017 10)
Mehta RI,Tsymbalyuk N,Ivanova S,Stokum JA,Woo K,Gerzanich V,Simard JM

cDNA microarray analysis of spinal cord injury and regeneration related genes in rat.
Sheng li xue bao : [Acta physiologica Sinica]57(6)705-13(2005 Dec)
Xiao L,Ma ZL,Li X,Lin QX,Que HP,Liu SJ

Rat mesangial alpha-endosulfine.
Kidney international65(5)1731-9(2004 May)
Yee J,Cortes P,Barnes JL,Alviani R,Biederman JI,Szamosfalvi B

Gene expression of alpha-endosulfine in the rat brain: correlative changes with aging, learning and stress.
Journal of neurochemistry87(5)1086-100(2003 Dec)
Dou J,Cui C,Dufour F,Alkon DL,Zhao WQ

Localization of alpha-endosulphine in pancreatic somatostatin delta cells and expression during rat pancreas development.
Diabetologia45(5)703-10(2002 May)
Gros L,Br?ant B,Duchene B,Leroy C,Fauconnier G,Bataille D,Virsolvy A