Acan cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Acan
Entrez Gene ID	58968
Full Name	aggrecan
Synonyms	Agc,Agc1
General protein information	Preferred Names aggrecan Names aggrecan core protein CSPCP aggrecan 1 aggrecan, structural proteoglycan of cartilage cartilage-specific proteoglycan core protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q31
Summary	an extracellular matrix proteoglycan; cleaved by ADAMTS1 [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022190.1	NP_071526.1	aggrecan core protein precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ACAN	NP_776406.1
Mus musculus (house mouse)	Acan	NP_031450.2
Pan troglodytes (chimpanzee)	ACAN	XP_003952775.1
Rattus norvegicus (Norway rat)	Acan	NP_071526.1
	ACAN	XP_002804990.1

Gene Ontology
Bibliography

Related articles in PubMed

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Barallobre-Barreiro J, Oklu R, Lynch M, Fava M, Baig F, Yin X, Barwari T, Potier DN, Albadawi H, Jahangiri M, Porter KE, Watkins MT, Misra S, Stoughton J, Mayr M
Cardiovascular research110(3)419-30(2016 06)

Transforming growth factor-?1 induces type II collagen and aggrecan expression via activation of extracellular signal-regulated kinase 1/2 and Smad2/3 signaling pathways.
Zhu Y, Tao H, Jin C, Liu Y, Lu X, Hu X, Wang X
Molecular medicine reports12(4)5573-9(2015 Oct)

Idiopathic short stature due to novel heterozygous mutation of the aggrecan gene.
Quintos JB, Guo MH, Dauber A
Journal of pediatric endocrinology & metabolism : JPEM28(7-8)927-32(2015 Jul)

Global genetic analysis in mice unveils central role for cilia in congenital heart disease.
Li Y, Klena NT, Gabriel GC, Liu X, Kim AJ, Lemke K, Chen Y, Chatterjee B, Devine W, Damerla RR, Chang C, Yagi H, San Agustin JT, Thahir M, Anderton S, Lawhead C, Vescovi A, Pratt H, Morgan J, Haynes L, Smith CL, Eppig JT, Reinholdt L, Francis R, Leatherbury L, Ganapathiraju MK, Tobita K, Pazour GJ, Lo CW
Nature521(7553)520-4(2015 May)

Expression of hedgehog signal pathway in articular cartilage is associated with the severity of cartilage damage in rats with adjuvant-induced arthritis.
Li R, Cai L, Hu CM, Wu TN, Li J
Journal of inflammation (London, England)1224(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The ERK1/2 and Smad2/3 signaling pathways regulate type II collagen and aggrecan expression in rat chondrocytes.
Title: Transforming growth factor-?1 induces type II collagen and aggrecan expression via activation of extracellular signal-regulated kinase 1/2 and Smad2/3 signaling pathways.

To elucidate the transcriptional mechanism that leads to abundant expression of aggrecan by chondrocytes, it screened for enhancer elements in 130 kb of the aggrecan gene, Agc1, using a reporter assay system that we previously developed.
Title: A candidate enhancer element responsible for high-level expression of the aggrecan gene in chondrocytes.

Local adipose tissue stem cells protected the bronchial stump after pneumonectomy and induced local changes in Agc1 gene expression.
Title: Stem cells protect the bronchial stump in rat, increasing Sox6, Col2a1, and Agc1 expression.

These results support a role for MafB as a regulator of chondrocyte gene expression and matrix formation via control of aggrecan, MMP3 and MMP13 expression, and indicate an important role for RA in the regulation of MafB
Title: Retinoic acid and the transcription factor MafB act together and differentially to regulate aggrecan and matrix metalloproteinase gene expression in neonatal chondrocytes.

Data suggest that expression of aggrecan and matrix metallopeptidase 13 in femur of female neonatal rats is altered by maternal vitamin A status and neonatal diet (i.e., supplementation with vitamin A and retinoic acid).
Title: Perinatal exposure to vitamin A differentially regulates chondrocyte growth and the expression of aggrecan and matrix metalloprotein genes in the femur of neonatal rats.

The following Acan gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Acan cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05394	NM_022190.1 Latest version!	Rattus norvegicus aggrecan (Acan), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Acan ( NM_022190.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071526.1 Rattus norvegicus aggrecan (Acan), mRNA.

Price & Availability↑

CloneID	ORa05394
Clone ID Related Accession (Same CDS sequence)	NM_022190.1
Accession Version	NM_022190.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Rattus norvegicus(Norway rat)
Product	aggrecan core protein precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J03485.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J03485.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGACCACTT TACTCTTGGT CTTTGTGACT CTGCGGGTCA TCGCTGCAGT GATCTCAGAA 
GAAGTTCCAG ACCATGACAA CTCACTGAGT GTGAGCATCC CTCAACCATC CCCACTGAAG 
GCCCTCCTAG GGACTTCCCT CACCATCCCC TGCTACTTCA TCGACCCCAT GCATCCTGTG 
ACCACTGCTC CCTCCACTGC CCCCCTCACC CCAAGAATCA AGTGGAGCCG TGTTTCCAAG 
GAAAAGGAGG TGGTACTGTT GGTGGCCACT GAAGGACAGG TTCGAGTGAA CAGCATCTAC 
CAAGACAAGG TGTCACTTCC CAACTATCCA GCCATCCCCA GCGATGCCAC CTTGGAAATC 
CAGAACCTTC GCTCCAATGA CTCTGGGATC TATCGGTGTG AAGTGATGCA TGGCATTGAG 
GACAGCGAAG CCACCCTGGA GGTCATAGTA AAAGGCATTG TGTTCCACTA CAGAGCTATT 
TCCACACGCT ACACACTGGA CTTTGACCGA GCCCAGCGGG CTTGCCTTCA GAACAGCGCC 
ATCATCGCCA CCCCTGAGCA ACTGCAGGCC GCCTATGAGG ATGGCTTCCA CCAGTGTGAT 
GCAGGCTGGC TGGCTGATCA GACAGTCAGA TACCCCATCC ACACTCCCCG GGAAGGTTGC 
TATGGTGACA AGGACGAGTT CCCTGGAGTG AGGACCTATG GAATCCGAGA CACCAACGAG 
ACCTATGATG TGTACTGCTT CGCTGAAGAG ATGGAGGGTG AGGTCTTTTA TGCCACATCC 
CCGGAGAAAT TCACCTTCCA GGAGGCAGCC AACGAGTGCC GGAGGCTGGG GGCACGGTTG 
GCCACCACAG GCCAGCTCTA CCTTGCCTGG CAGGGCGGTA TGGACATGTG CAGCGCTGGC 
TGGCTGGCGG ACCGCAGCGT TCGCTACCCC ATCTCCAAGG CTCGGCCCAA CTGCGGAGGC 
AACCTCCTGG GTGTAAGGAC TGTCTATCTG CACGCCAACC AGACAGGCTA CCCTGATCCC 
TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTAGACAT CCCAGAAAAC 
TTCTTCGGAG TGGGTGGTGA AGAGGACATC ACCATCCAGA CAGTGACCTG GCCAGATCTG 
GAGCTGCCCC TGCCCCGTAA TATTACGGAG GGAGAAGCCC GGGGCAATGT GATCCTCACT 
GCAAAGCCCA TCTTCGACAT GTCCCCCACT GTCTCAGAGC CTGGGGAGGC CCTCACACTT 
GCCCCTGAAG TGGGGACCAC AGTCTTCCCT GAGGCTGGGG AGAGAACTGA AAAGACCACC 
AGGCCCTGGG GCTTTCCCGA GGAAGCCACA CGTGGGCCTG ATTCTGCCAC TGCCTTCGCC 
AGTGAGGACC TGGTGGTGCG AGTGACCATC TCTCCAGGTG CAGTTGAGGT CCCTGGTCAG 
CCCCGCTTGC CAGGGGGAGT TGTATTCCAC TACCGCCCGG GCTCTACCAG ATACTCTCTG 
ACATTTGAGG AGGCACAGCA GGCCTGCATT CGCACGGGAG CAGCCATAGC TTCTCCTGAG 
CAACTCCAGG CTGCCTATGA GGCAGGCTAT GAGCAGTGTG ATGCTGGCTG GCTGCAGGAC 
CAGACTGTCA GATACCCCAT TGTGAGCCCA CGGACCCCAT GTGTGGGTGA CAAGGACAGC 
AGCCCCGGAG TCAGGACCTA TGGCGTGCGC CCATCATCAG AAACCTATGA TGTCTACTGC 
TACGTGGACA AGCTTGAGGG GGAAGTGTTC TTCGCCACAC AAATGGAGCA GTTCACCTTC 
CAGGAAGCTC AGGCCTTCTG TGCAGCTCAA AATGCTACCC TGGCCTCCAC TGGCCAGCTC 
TACGCTGCCT GGAGCCAGGG CCTGGACAAG TGCTATGCTG GCTGGTTGGC AGATGGCACC 
CTCCGATACC CCATCGTCAA CCCTCGGCCT GCCTGTGGTG GTGACAAGCC GGGCGTGAGA 
ACTGTCTACC TCTACCCCAA CCAGACCGGC CTCCCTGATC CACTGTCCAA GCACCATGCC 
TTCTGCTTCC GAGGTGTGTC AGTGGTGCCC TCTCCAGGTG GTACACCCAC ATCGCCCTCT 
GACATAGAGG ACTGGATCGT CACTCGAGTG GAGCCTGGCG TGGATGCTGT CCCCTTGGAG 
CCGGAGACGA CAGAAGTACC ATATTTCACC ACTGAGCCAG AAAAACAGAC TGAATGGGAG 
CCAGCCTACA CCCCAGTGGG CACATCCCCA TTGCCAGGGA TCCCTCCCAC ATGGCTTCCC 
ACCGTCCCAG CAGCAGAGGA ACACACCGAA AGTCCCTCTG CCTCTCAAGA GCCCTCTGCC 
TCGCAAGTCC CTTCCACATC AGAAGAGCCA TACACACCTT CACTTGCAGT GCCAAGCGGG 
ACCGAGCTGC CCAGCTCAGG GGACACATCA GGGGCACCTG ACCTCAGTGG TGACTTCACA 
GGCAGCACAG ACACTTCAGG ACGCCTTGAC TCCAGTGGGG AGCCATCAGG AGGCAGTGAA 
AGTGGACTTC CCTCTGGCGA CCTTGACTCC AGTGGTCTCG GCCCCACAGT GAGCTCAGGC 
TTGCCTGTAG AAAGTGGGTC AGCCTCGGGA GATGGAGAAA TCCCCTGGTC GAGCACTCCC 
ACAGTTGACA GACTGCCCTC TGGAGGTGAG AGCCTTGAAG GTTCTGCCTC TGCCTCTGGA 
ACAGGAGACC TTAGTGGGCT GCCTTCAGGA GGGGAAATTA CAGAAACTTC TGCTTCTGGG 
ACAGAAGAAA TCAGTGGACT TCCTTCTGGA GGAGACGATC TAGAAACTTC TACCTCTGGA 
ATAGATGGAG CCAGTGTGCT TCCTACTGGA AGAGGAGGTC TAGAGACGTC TGCCTCTGGA 
GTAGAGGACC TCAGTGGACT TCCTTCTGGA GAAGAAGGTT CAGAAACATC TACCTCTGGA 
ATAGAGGACA TTAGTGTACT TCCGACTGGA GAAAGTCCAG AAACTTCTGC TTCTGGAGTG 
GGAGACTTGA GTGGACTTCC CTCAGGAGGA GAAAGTCTAG AAACATCTGC TTCAGGTGTA 
GAGGATGTCA CCCAGCTTCC TACTGAAAGA GGAGGTCTAG AGACTTCTGC CTCTGGAATA 
GAGGACATCA CTGTGCTTCC TACTGGAAGA GAAAATCTAG AAACTTCTGC CTCTGGAGTA 
GAGGATGTCA GTGGACTTCC TTCTGGAAAA GAAGGTCTAG AGACTTCTGC CTCTGGAATA 
GAGGACATTA GTGTGTTCCC TACTGAAGCA GAAGGTCTAG AAACTTCTGC CTCCGGGGGA 
TATGTCAGTG GGATTCCTTC TGGAGAAGAT GGTACAGAAA CCTCTACTTC TGGAGTAGAG 
GGTGTGAGTG GTCTTCCTTC TGGAGGAGAG GGTCTAGAAA CGTCTGCCTC TGGAGTAGAA 
GATCTTGGTC TTCCTACAAG AGATAGTCTA GAGACTTCTG CCTCAGGAGT AGATGTTACT 
GGGTATCCTT CTGGAAGAGA GGACACAGAA ACCTCTGTTC CTGGGGTAGG TGATGACCTT 
AGTGGACTTC CTTCTGGACA AGAAGGCCTA GAGACCTCAG CTTCAGGAGC TGAGGACCTT 
GGTGGCTTGC CTTCTGGAAA AGAAGACTTG GTAGGGTCTG CTTCTGGAGC CCTGGACTTC 
GGCAAGCTAC CTTCTGGAAC TCTAGGAAGT GGTCAAACTC CAGAAGCTAG TGGCCTTCCC 
TCTGGATTTA GTGGTGAATA TTCTGGAGTG GACATTGGAA GTGGCCCATC CTCTGGCCTG 
CCTGACTTTA GTGGACTTCC ATCCGGCTTC CCAACAGTCT CCCTTGTGGA CAGTACCTTA 
GTGGAAGTGA TCACAGCCAC CACTGCCAGT GAACTGGAAG GAAGGGGGAC CATCAGCGTC 
AGTGGTTCAG GAGAAGAGTC AGGGCCGCCC CTCAGTGAGT TGGACAGTAG TGCGGACATT 
AGTGGTCTCC CTTCAGGAAC TGAACTCAGT GGCCAAACAT CCGGATCTCT TGATGTCAGT 
GGAGAAACAT CTGGATTTTT TGATGTTAGT GGACAGCCAT TTGGGTCTTC TGGCACTGGT 
GAGGGAACAT CTGGGATTCC TGAAGTCAGT GGACAGGCCG TCAGGAGTCC TGACACCACT 
GAGATATCTG AGCTTAGTGG ACTGTCCTCT GGGCAACCAG ATGTCAGTGG AGAAGGGTCT 
GGAATTCTCT TTGGCAGTGG CCAATCCTCT GGCATAACAT CTGTGAGTGG AGAAACCTCT 
GGGATTTCTG ATCTCAGTGG GCAACCTTCA GGGTTCCCAG TGCTCAGCGG AACAACACCT 
GGAACCCCTG ACCTGGCTTC TGGCGCCATG AGTGGCAGTG GAGATTCTTC TGGCATTACG 
TTTGTGGACA CCAGTCTTAT TGAAGTGACC CCTACCACAT TTAGAGAAGA AGAGGGGTTA 
GGGTCTGTGG AACTCAGTGG CCTCCCTTCT GGGGAGACAG ATCTGTCTGG CACATCTGGG 
ATGGTGGATG TCAGTGGACA ATCTTCTGGA GCAATCGATT CCAGTGGACT CATATCCCCC 
ACTCCAGAAT TCAGTGGCCT CCCAAGCGGA GTAGCTGAGG TCAGTGGAGA GGTCTCTGGA 
GTTGAGACTG GGAGCAGCTT GTCCTCGGGA GCATTCGATG GCAGTGGACT TGTCTCAGGT 
TTCCCTACGG TGTCTCTTGT AGACAGAACT TTGGTGGAGT CTATAACCCT GGCTCCTACT 
GCTCAAGAAG CTGGAGAAGG GCCTTCCAGC ATTTTGGAAT TCAGTGGTGC CCATTCTGGA 
ACACCAGACA TATCTGGGGA CCTCTCTGGG TCTTTAGACC AAAGCACATG GCAGCCTGGG 
TGGACAGAAG CCAGCACAGA GCCACCAAGC TCCCCATATT TTAGTGGGGA CTTCTCTAGC 
ACCACTGATG CAAGTGGAGA ATCCATAACT GCCCCAACTG GCAGTGGGGA AACCTCGGGG 
CTTCCGGAAG TCACTTTAAT CACTTCAGAG TTAGTGGAGG GCGTGACTGA ACCAACTGTT 
TCCCAGGAAC TTGGCCATGG TCCTTCTATG ACATATACTC CCCGGCTCTT TGAGGCCAGT 
GGGGAAGCCT CAGCATCTGG GGACCTTGGT GGACCTGTAA CAATCTTTCC CGGGTCTGGG 
GTAGAAGCTT CAGTCCCAGA AGGCAGCAGT GACCCATCTG CTTACCCTGA GGCTGGAGTG 
GGAGTGTCTG CTGCCCCTGA GGCCAGCAGT CAATTGTCTG AGTTCCCAGA TCTGCATGGA 
ATCACCTCTG CCTCCCGTGA AACAGATCTG GAGATGACAA CCCCAGGCAC GGAGGTGAGC 
AGCAACCCAT GGACCTTTCA GGAAGGCACC AGGGAGGGAT CCGCTGCTCC AGAAGTGAGT 
GGAGAATCTA GCACTACCTC CGACATAGAT GCAGGCACTT CAGGTGTGCC TTTTGCCACG 
CCCATGACTT CTGGAGACAG GACTGAAATC AGTGGAGAAT GGTCTGATCA TACCTCAGAG 
GTGAATGTTA CCGTCAGCAC CACCGTCCCA GAGTCCAGGT GGGCCCAGTC TACCCAGCAC 
CCTACAGAGA CACTTCAAGA AATCGGATCC CCAAATCCCT CATACTCAGG AGAAGAGACC 
CAAACAGCAG AAACAGCCAA GTCCCTGACA GACACCCCTA CCCTTGCTTC TCCAGAAGGG 
TCAGGAGAAA CAGAGTCAAC CGCTGCAGAC ATTGATGAGT GCCTCTCAAG CCCTTGTCTG 
AATGGAGCCA CCTGCGTGGA TGCCTTGGAC ACTTTCACAT GCTTATGCCT TCCGAGCTAC 
AGAGGGGACC TGTGTGAGAT CGACCAGGAG CAATGTGAGG AGGGGTGGAC CAAGTTCCAG 
GGCCACTGTT ATCGCCACTT TCCCGACCGC GAGACCTGGG TGGATGCAGA GAGACGGTGT 
CGGGAGCAGC AGTCACATCT GAGCAGCATC GTCACCCCTG AGGAACAGGA GTTCGTCAAC 
AAAAATGCTC AAGACTACCA GTGGATCGGT CTGAATGACA GAACCATCGA AGGGGACTTC 
CGCTGGTCTG ATGGACACTC CCTGCAATTT GAGAAGTGGC GTCCAAACCA ACCCGACAAT 
TTCTTTGCCA CTGGAGAGGA CTGCGTAGTG ATGATCTGGC ATGAGAGGGG TGAATGGAAC 
GATGTCCCCT GCAATTACCA GCTGCCCTTC ACATGTAAAA AGGGCACAGT GGCCTGTGGA 
GAACCCCCAG CGGTGGAGCA TGCCAGAACC CTTGGGCAGA AGAAAGATCG CTATGAGATC 
AGCTCCCTGG TGCGGTACCA GTGTACTGAG GGCTTTGTCC AGCGCCATGT GCCCACCATC 
CGGTGTCAGC CCAGTGCGGA CTGGGAAGAG CCTCGAATCA CTTGCACAGA CCCCAACACC 
TACAAGCACA GGCTACAGAA GCGGACGATG AGACCCACAC GGAGGAGCCG CCCCAGCATG 
GCCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071526.1
CDS	389..6877
Translation	MTTLLLVFVTLRVIAAVISEEVPDHDNSLSVSIPQPSPLKALLGTSLTIPCYFIDPMHPVTTAPSTAPLTPRIKWSRVSKEKEVVLLVATEGQVRVNSIYQDKVSLPNYPAIPSDATLEIQNLRSNDSGIYRCEVMHGIEDSEATLEVIVKGIVFHYRAISTRYTLDFDRAQRACLQNSAIIATPEQLQAAYEDGFHQCDAGWLADQTVRYPIHTPREGCYGDKDEFPGVRTYGIRDTNETYDVYCFAEEMEGEVFYATSPEKFTFQEAANECRRLGARLATTGQLYLAWQGGMDMCSAGWLADRSVRYPISKARPNCGGNLLGVRTVYLHANQTGYPDPSSRYDAICYTGEDFVDIPENFFGVGGEEDITIQTVTWPDLELPLPRNITEGEARGNVILTAKPIFDMSPTVSEPGEALTLAPEVGTTVFPEAGERTEKTTRPWGFPEEATRGPDSATAFASEDLVVRVTISPGAVEVPGQPRLPGGVVFHYRPGSTRYSLTFEEAQQACIRTGAAIASPEQLQAAYEAGYEQCDAGWLQDQTVRYPIVSPRTPCVGDKDSSPGVRTYGVRPSSETYDVYCYVDKLEGEVFFATQMEQFTFQEAQAFCAAQNATLASTGQLYAAWSQGLDKCYAGWLADGTLRYPIVNPRPACGGDKPGVRTVYLYPNQTGLPDPLSKHHAFCFRGVSVVPSPGGTPTSPSDIEDWIVTRVEPGVDAVPLEPETTEVPYFTTEPEKQTEWEPAYTPVGTSPLPGIPPTWLPTVPAAEEHTESPSASQEPSASQVPSTSEEPYTPSLAVPSGTELPSSGDTSGAPDLSGDFTGSTDTSGRLDSSGEPSGGSESGLPSGDLDSSGLGPTVSSGLPVESGSASGDGEIPWSSTPTVDRLPSGGESLEGSASASGTGDLSGLPSGGEITETSASGTEEISGLPSGGDDLETSTSGIDGASVLPTGRGGLETSASGVEDLSGLPSGEEGSETSTSGIEDISVLPTGESPETSASGVGDLSGLPSGGESLETSASGVEDVTQLPTERGGLETSASGIEDITVLPTGRENLETSASGVEDVSGLPSGKEGLETSASGIEDISVFPTEAEGLETSASGGYVSGIPSGEDGTETSTSGVEGVSGLPSGGEGLETSASGVEDLGLPTRDSLETSASGVDVTGYPSGREDTETSVPGVGDDLSGLPSGQEGLETSASGAEDLGGLPSGKEDLVGSASGALDFGKLPSGTLGSGQTPEASGLPSGFSGEYSGVDIGSGPSSGLPDFSGLPSGFPTVSLVDSTLVEVITATTASELEGRGTISVSGSGEESGPPLSELDSSADISGLPSGTELSGQTSGSLDVSGETSGFFDVSGQPFGSSGTGEGTSGIPEVSGQAVRSPDTTEISELSGLSSGQPDVSGEGSGILFGSGQSSGITSVSGETSGISDLSGQPSGFPVLSGTTPGTPDLASGAMSGSGDSSGITFVDTSLIEVTPTTFREEEGLGSVELSGLPSGETDLSGTSGMVDVSGQSSGAIDSSGLISPTPEFSGLPSGVAEVSGEVSGVETGSSLSSGAFDGSGLVSGFPTVSLVDRTLVESITLAPTAQEAGEGPSSILEFSGAHSGTPDISGDLSGSLDQSTWQPGWTEASTEPPSSPYFSGDFSSTTDASGESITAPTGSGETSGLPEVTLITSELVEGVTEPTVSQELGHGPSMTYTPRLFEASGEASASGDLGGPVTIFPGSGVEASVPEGSSDPSAYPEAGVGVSAAPEASSQLSEFPDLHGITSASRETDLEMTTPGTEVSSNPWTFQEGTREGSAAPEVSGESSTTSDIDAGTSGVPFATPMTSGDRTEISGEWSDHTSEVNVTVSTTVPESRWAQSTQHPTETLQEIGSPNPSYSGEETQTAETAKSLTDTPTLASPEGSGETESTAADIDECLSSPCLNGATCVDALDTFTCLCLPSYRGDLCEIDQEQCEEGWTKFQGHCYRHFPDRETWVDAERRCREQQSHLSSIVTPEEQEFVNKNAQDYQWIGLNDRTIEGDFRWSDGHSLQFEKWRPNQPDNFFATGEDCVVMIWHERGEWNDVPCNYQLPFTCKKGTVACGEPPAVEHARTLGQKKDRYEISSLVRYQCTEGFVQRHVPTIRCQPSADWEEPRITCTDPNTYKHRLQKRTMRPTRRSRPSMAH

Target ORF information:

RefSeq Version	NM_022190.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus aggrecan (Acan), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022190.1

ORF Insert Sequence:

ATGACCACTT TACTCTTGGT CTTTGTGACT CTGCGGGTCA TCGCTGCAGT GATCTCAGAA 
GAAGTTCCAG ACCATGACAA CTCACTGAGT GTGAGCATCC CTCAACCATC CCCACTGAAG 
GCCCTCCTAG GGACTTCCCT CACCATCCCC TGCTACTTCA TCGACCCCAT GCATCCTGTG 
ACCACTGCTC CCTCCACTGC CCCCCTCACC CCAAGAATCA AGTGGAGCCG TGTTTCCAAG 
GAAAAGGAGG TGGTACTGTT GGTGGCCACT GAAGGACAGG TTCGAGTGAA CAGCATCTAC 
CAAGACAAGG TGTCACTTCC CAACTATCCA GCCATCCCCA GCGATGCCAC CTTGGAAATC 
CAGAACCTTC GCTCCAATGA CTCTGGGATC TATCGGTGTG AAGTGATGCA TGGCATTGAG 
GACAGCGAAG CCACCCTGGA GGTCATAGTA AAAGGCATTG TGTTCCACTA CAGAGCTATT 
TCCACACGCT ACACACTGGA CTTTGACCGA GCCCAGCGGG CTTGCCTTCA GAACAGCGCC 
ATCATCGCCA CCCCTGAGCA ACTGCAGGCC GCCTATGAGG ATGGCTTCCA CCAGTGTGAT 
GCAGGCTGGC TGGCTGATCA GACAGTCAGA TACCCCATCC ACACTCCCCG GGAAGGTTGC 
TATGGTGACA AGGACGAGTT CCCTGGAGTG AGGACCTATG GAATCCGAGA CACCAACGAG 
ACCTATGATG TGTACTGCTT CGCTGAAGAG ATGGAGGGTG AGGTCTTTTA TGCCACATCC 
CCGGAGAAAT TCACCTTCCA GGAGGCAGCC AACGAGTGCC GGAGGCTGGG GGCACGGTTG 
GCCACCACAG GCCAGCTCTA CCTTGCCTGG CAGGGCGGTA TGGACATGTG CAGCGCTGGC 
TGGCTGGCGG ACCGCAGCGT TCGCTACCCC ATCTCCAAGG CTCGGCCCAA CTGCGGAGGC 
AACCTCCTGG GTGTAAGGAC TGTCTATCTG CACGCCAACC AGACAGGCTA CCCTGATCCC 
TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTAGACAT CCCAGAAAAC 
TTCTTCGGAG TGGGTGGTGA AGAGGACATC ACCATCCAGA CAGTGACCTG GCCAGATCTG 
GAGCTGCCCC TGCCCCGTAA TATTACGGAG GGAGAAGCCC GGGGCAATGT GATCCTCACT 
GCAAAGCCCA TCTTCGACAT GTCCCCCACT GTCTCAGAGC CTGGGGAGGC CCTCACACTT 
GCCCCTGAAG TGGGGACCAC AGTCTTCCCT GAGGCTGGGG AGAGAACTGA AAAGACCACC 
AGGCCCTGGG GCTTTCCCGA GGAAGCCACA CGTGGGCCTG ATTCTGCCAC TGCCTTCGCC 
AGTGAGGACC TGGTGGTGCG AGTGACCATC TCTCCAGGTG CAGTTGAGGT CCCTGGTCAG 
CCCCGCTTGC CAGGGGGAGT TGTATTCCAC TACCGCCCGG GCTCTACCAG ATACTCTCTG 
ACATTTGAGG AGGCACAGCA GGCCTGCATT CGCACGGGAG CAGCCATAGC TTCTCCTGAG 
CAACTCCAGG CTGCCTATGA GGCAGGCTAT GAGCAGTGTG ATGCTGGCTG GCTGCAGGAC 
CAGACTGTCA GATACCCCAT TGTGAGCCCA CGGACCCCAT GTGTGGGTGA CAAGGACAGC 
AGCCCCGGAG TCAGGACCTA TGGCGTGCGC CCATCATCAG AAACCTATGA TGTCTACTGC 
TACGTGGACA AGCTTGAGGG GGAAGTGTTC TTCGCCACAC AAATGGAGCA GTTCACCTTC 
CAGGAAGCTC AGGCCTTCTG TGCAGCTCAA AATGCTACCC TGGCCTCCAC TGGCCAGCTC 
TACGCTGCCT GGAGCCAGGG CCTGGACAAG TGCTATGCTG GCTGGTTGGC AGATGGCACC 
CTCCGATACC CCATCGTCAA CCCTCGGCCT GCCTGTGGTG GTGACAAGCC GGGCGTGAGA 
ACTGTCTACC TCTACCCCAA CCAGACCGGC CTCCCTGATC CACTGTCCAA GCACCATGCC 
TTCTGCTTCC GAGGTGTGTC AGTGGTGCCC TCTCCAGGTG GTACACCCAC ATCGCCCTCT 
GACATAGAGG ACTGGATCGT CACTCGAGTG GAGCCTGGCG TGGATGCTGT CCCCTTGGAG 
CCGGAGACGA CAGAAGTACC ATATTTCACC ACTGAGCCAG AAAAACAGAC TGAATGGGAG 
CCAGCCTACA CCCCAGTGGG CACATCCCCA TTGCCAGGGA TCCCTCCCAC ATGGCTTCCC 
ACCGTCCCAG CAGCAGAGGA ACACACCGAA AGTCCCTCTG CCTCTCAAGA GCCCTCTGCC 
TCGCAAGTCC CTTCCACATC AGAAGAGCCA TACACACCTT CACTTGCAGT GCCAAGCGGG 
ACCGAGCTGC CCAGCTCAGG GGACACATCA GGGGCACCTG ACCTCAGTGG TGACTTCACA 
GGCAGCACAG ACACTTCAGG ACGCCTTGAC TCCAGTGGGG AGCCATCAGG AGGCAGTGAA 
AGTGGACTTC CCTCTGGCGA CCTTGACTCC AGTGGTCTCG GCCCCACAGT GAGCTCAGGC 
TTGCCTGTAG AAAGTGGGTC AGCCTCGGGA GATGGAGAAA TCCCCTGGTC GAGCACTCCC 
ACAGTTGACA GACTGCCCTC TGGAGGTGAG AGCCTTGAAG GTTCTGCCTC TGCCTCTGGA 
ACAGGAGACC TTAGTGGGCT GCCTTCAGGA GGGGAAATTA CAGAAACTTC TGCTTCTGGG 
ACAGAAGAAA TCAGTGGACT TCCTTCTGGA GGAGACGATC TAGAAACTTC TACCTCTGGA 
ATAGATGGAG CCAGTGTGCT TCCTACTGGA AGAGGAGGTC TAGAGACGTC TGCCTCTGGA 
GTAGAGGACC TCAGTGGACT TCCTTCTGGA GAAGAAGGTT CAGAAACATC TACCTCTGGA 
ATAGAGGACA TTAGTGTACT TCCGACTGGA GAAAGTCCAG AAACTTCTGC TTCTGGAGTG 
GGAGACTTGA GTGGACTTCC CTCAGGAGGA GAAAGTCTAG AAACATCTGC TTCAGGTGTA 
GAGGATGTCA CCCAGCTTCC TACTGAAAGA GGAGGTCTAG AGACTTCTGC CTCTGGAATA 
GAGGACATCA CTGTGCTTCC TACTGGAAGA GAAAATCTAG AAACTTCTGC CTCTGGAGTA 
GAGGATGTCA GTGGACTTCC TTCTGGAAAA GAAGGTCTAG AGACTTCTGC CTCTGGAATA 
GAGGACATTA GTGTGTTCCC TACTGAAGCA GAAGGTCTAG AAACTTCTGC CTCCGGGGGA 
TATGTCAGTG GGATTCCTTC TGGAGAAGAT GGTACAGAAA CCTCTACTTC TGGAGTAGAG 
GGTGTGAGTG GTCTTCCTTC TGGAGGAGAG GGTCTAGAAA CGTCTGCCTC TGGAGTAGAA 
GATCTTGGTC TTCCTACAAG AGATAGTCTA GAGACTTCTG CCTCAGGAGT AGATGTTACT 
GGGTATCCTT CTGGAAGAGA GGACACAGAA ACCTCTGTTC CTGGGGTAGG TGATGACCTT 
AGTGGACTTC CTTCTGGACA AGAAGGCCTA GAGACCTCAG CTTCAGGAGC TGAGGACCTT 
GGTGGCTTGC CTTCTGGAAA AGAAGACTTG GTAGGGTCTG CTTCTGGAGC CCTGGACTTC 
GGCAAGCTAC CTTCTGGAAC TCTAGGAAGT GGTCAAACTC CAGAAGCTAG TGGCCTTCCC 
TCTGGATTTA GTGGTGAATA TTCTGGAGTG GACATTGGAA GTGGCCCATC CTCTGGCCTG 
CCTGACTTTA GTGGACTTCC ATCCGGCTTC CCAACAGTCT CCCTTGTGGA CAGTACCTTA 
GTGGAAGTGA TCACAGCCAC CACTGCCAGT GAACTGGAAG GAAGGGGGAC CATCAGCGTC 
AGTGGTTCAG GAGAAGAGTC AGGGCCGCCC CTCAGTGAGT TGGACAGTAG TGCGGACATT 
AGTGGTCTCC CTTCAGGAAC TGAACTCAGT GGCCAAACAT CCGGATCTCT TGATGTCAGT 
GGAGAAACAT CTGGATTTTT TGATGTTAGT GGACAGCCAT TTGGGTCTTC TGGCACTGGT 
GAGGGAACAT CTGGGATTCC TGAAGTCAGT GGACAGGCCG TCAGGAGTCC TGACACCACT 
GAGATATCTG AGCTTAGTGG ACTGTCCTCT GGGCAACCAG ATGTCAGTGG AGAAGGGTCT 
GGAATTCTCT TTGGCAGTGG CCAATCCTCT GGCATAACAT CTGTGAGTGG AGAAACCTCT 
GGGATTTCTG ATCTCAGTGG GCAACCTTCA GGGTTCCCAG TGCTCAGCGG AACAACACCT 
GGAACCCCTG ACCTGGCTTC TGGCGCCATG AGTGGCAGTG GAGATTCTTC TGGCATTACG 
TTTGTGGACA CCAGTCTTAT TGAAGTGACC CCTACCACAT TTAGAGAAGA AGAGGGGTTA 
GGGTCTGTGG AACTCAGTGG CCTCCCTTCT GGGGAGACAG ATCTGTCTGG CACATCTGGG 
ATGGTGGATG TCAGTGGACA ATCTTCTGGA GCAATCGATT CCAGTGGACT CATATCCCCC 
ACTCCAGAAT TCAGTGGCCT CCCAAGCGGA GTAGCTGAGG TCAGTGGAGA GGTCTCTGGA 
GTTGAGACTG GGAGCAGCTT GTCCTCGGGA GCATTCGATG GCAGTGGACT TGTCTCAGGT 
TTCCCTACGG TGTCTCTTGT AGACAGAACT TTGGTGGAGT CTATAACCCT GGCTCCTACT 
GCTCAAGAAG CTGGAGAAGG GCCTTCCAGC ATTTTGGAAT TCAGTGGTGC CCATTCTGGA 
ACACCAGACA TATCTGGGGA CCTCTCTGGG TCTTTAGACC AAAGCACATG GCAGCCTGGG 
TGGACAGAAG CCAGCACAGA GCCACCAAGC TCCCCATATT TTAGTGGGGA CTTCTCTAGC 
ACCACTGATG CAAGTGGAGA ATCCATAACT GCCCCAACTG GCAGTGGGGA AACCTCGGGG 
CTTCCGGAAG TCACTTTAAT CACTTCAGAG TTAGTGGAGG GCGTGACTGA ACCAACTGTT 
TCCCAGGAAC TTGGCCATGG TCCTTCTATG ACATATACTC CCCGGCTCTT TGAGGCCAGT 
GGGGAAGCCT CAGCATCTGG GGACCTTGGT GGACCTGTAA CAATCTTTCC CGGGTCTGGG 
GTAGAAGCTT CAGTCCCAGA AGGCAGCAGT GACCCATCTG CTTACCCTGA GGCTGGAGTG 
GGAGTGTCTG CTGCCCCTGA GGCCAGCAGT CAATTGTCTG AGTTCCCAGA TCTGCATGGA 
ATCACCTCTG CCTCCCGTGA AACAGATCTG GAGATGACAA CCCCAGGCAC GGAGGTGAGC 
AGCAACCCAT GGACCTTTCA GGAAGGCACC AGGGAGGGAT CCGCTGCTCC AGAAGTGAGT 
GGAGAATCTA GCACTACCTC CGACATAGAT GCAGGCACTT CAGGTGTGCC TTTTGCCACG 
CCCATGACTT CTGGAGACAG GACTGAAATC AGTGGAGAAT GGTCTGATCA TACCTCAGAG 
GTGAATGTTA CCGTCAGCAC CACCGTCCCA GAGTCCAGGT GGGCCCAGTC TACCCAGCAC 
CCTACAGAGA CACTTCAAGA AATCGGATCC CCAAATCCCT CATACTCAGG AGAAGAGACC 
CAAACAGCAG AAACAGCCAA GTCCCTGACA GACACCCCTA CCCTTGCTTC TCCAGAAGGG 
TCAGGAGAAA CAGAGTCAAC CGCTGCAGAC ATTGATGAGT GCCTCTCAAG CCCTTGTCTG 
AATGGAGCCA CCTGCGTGGA TGCCTTGGAC ACTTTCACAT GCTTATGCCT TCCGAGCTAC 
AGAGGGGACC TGTGTGAGAT CGACCAGGAG CAATGTGAGG AGGGGTGGAC CAAGTTCCAG 
GGCCACTGTT ATCGCCACTT TCCCGACCGC GAGACCTGGG TGGATGCAGA GAGACGGTGT 
CGGGAGCAGC AGTCACATCT GAGCAGCATC GTCACCCCTG AGGAACAGGA GTTCGTCAAC 
AAAAATGCTC AAGACTACCA GTGGATCGGT CTGAATGACA GAACCATCGA AGGGGACTTC 
CGCTGGTCTG ATGGACACTC CCTGCAATTT GAGAAGTGGC GTCCAAACCA ACCCGACAAT 
TTCTTTGCCA CTGGAGAGGA CTGCGTAGTG ATGATCTGGC ATGAGAGGGG TGAATGGAAC 
GATGTCCCCT GCAATTACCA GCTGCCCTTC ACATGTAAAA AGGGCACAGT GGCCTGTGGA 
GAACCCCCAG CGGTGGAGCA TGCCAGAACC CTTGGGCAGA AGAAAGATCG CTATGAGATC 
AGCTCCCTGG TGCGGTACCA GTGTACTGAG GGCTTTGTCC AGCGCCATGT GCCCACCATC 
CGGTGTCAGC CCAGTGCGGA CTGGGAAGAG CCTCGAATCA CTTGCACAGA CCCCAACACC 
TACAAGCACA GGCTACAGAA GCGGACGATG AGACCCACAC GGAGGAGCCG CCCCAGCATG 
GCCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Cardiovascular research110(3)419-30(2016 06)
Barallobre-Barreiro J,Oklu R,Lynch M,Fava M,Baig F,Yin X,Barwari T,Potier DN,Albadawi H,Jahangiri M,Porter KE,Watkins MT,Misra S,Stoughton J,Mayr M

Transforming growth factor-?1 induces type II collagen and aggrecan expression via activation of extracellular signal-regulated kinase 1/2 and Smad2/3 signaling pathways.
Molecular medicine reports12(4)5573-9(2015 Oct)
Zhu Y,Tao H,Jin C,Liu Y,Lu X,Hu X,Wang X

Idiopathic short stature due to novel heterozygous mutation of the aggrecan gene.
Journal of pediatric endocrinology & metabolism : JPEM28(7-8)927-32(2015 Jul)
Quintos JB,Guo MH,Dauber A

Global genetic analysis in mice unveils central role for cilia in congenital heart disease.
Nature521(7553)520-4(2015 May)
Li Y,Klena NT,Gabriel GC,Liu X,Kim AJ,Lemke K,Chen Y,Chatterjee B,Devine W,Damerla RR,Chang C,Yagi H,San Agustin JT,Thahir M,Anderton S,Lawhead C,Vescovi A,Pratt H,Morgan J,Haynes L,Smith CL,Eppig JT,Reinholdt L,Francis R,Leatherbury L,Ganapathiraju MK,Tobita K,Pazour GJ,Lo CW

Expression of hedgehog signal pathway in articular cartilage is associated with the severity of cartilage damage in rats with adjuvant-induced arthritis.
Journal of inflammation (London, England)1224(2015)
Li R,Cai L,Hu CM,Wu TN,Li J