Prkn cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prkn
Entrez Gene ID	56816
Full Name	parkin RBR E3 ubiquitin protein ligase
Synonyms	Park,Park2
General protein information	Preferred Names parkin RBR E3 ubiquitin protein ligase Names E3 ubiquitin-protein ligase parkin Parkinson disease (autosomal recessive, juvenile) 2, parkin parkin RBR E3 ubiquitin protein ligase 2 parkin variant SV5DEL parkinson protein 2, E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q11
Summary	localized to the cytoplasm in neurons of many regions of the brain; has N-terminal ubiquitin and ring-IBR-ring finger domains [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_020093.1	NP_064478.1	E3 ubiquitin-protein ligase parkin

Pathways from BioSystems

Homologies

	LOC710899	XP_001099588.2
Canis lupus familiaris (dog)	PARK2	XP_855152.2
Caenorhabditis elegans (roundworm)	pdr-1	NP_499846.2
Mus musculus (house mouse)	Park2	NP_057903.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006580	XP_316606.2
Homo sapiens (human)	PARK2	NP_004553.2
Danio rerio (zebrafish)	park2	NP_001017635.1
Pan troglodytes (chimpanzee)	PARK2	XP_001153913.1
Rattus norvegicus (Norway rat)	Park2	NP_064478.1
Gallus gallus (chicken)	PARK2	XP_004935613.1
Drosophila melanogaster (fruit fly)	park	NP_730601.1
Bos taurus (cattle)	PARK2	NP_001185994.1

Gene Ontology

The following Prkn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prkn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04720	NM_020093.1 Latest version!	Rattus norvegicus parkin RBR E3 ubiquitin protein ligase (Prkn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$272.30 $389.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prkn ( NM_020093.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_064478.1 Rattus norvegicus parkin RBR E3 ubiquitin protein ligase (Prkn), mRNA.

Price & Availability↑

CloneID	ORa04720
Clone ID Related Accession (Same CDS sequence)	NM_020093.1
Accession Version	NM_020093.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase parkin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB039878.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB039878.1, AF210434.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGATAGTGT TTGTCAGGTT CAACTCCAGC TATGGCTTCC CAGTGGAGGT CGATTCTGAC 
ACCAGCATCT TCCAGCTCAA GGAAGTGGTT GCTAAGCGAC AGGGGGTTCC AGCTGACCAG 
CTGCGAGTGA TTTTTGCTGG GAAGGAGCTG CAGAATCACC TGACAGTACA GAACTGTGAC 
CTGGAACAAC AGAGTATCGT TCACATAGTA CAGAGACCAC AGAGGAAAAG TCACGAAACA 
AATGCCTCTG GAGGGGACAA ACCCCAGAGC ACCCCGGAGG GCTCCATATG GGAGCCCAGA 
AGCTTGACTC GAGTGGACCT CAGCAGCCAT ATCCTGCCAG CGGACTCCGT GGGGCTGGCA 
GTCATTCTGG ACACAGACAG CAAGAGTGAC TCAGAAGCAG CCAGAGGTCC AGCAGCTAAA 
CCCACCTACC ACAGCTTTTT TGTCTACTGC AAAGGCCCCT GCCACAAGGT CCAGCCTGGG 
AAACTCCGAG TTCAGTGCGG CACCTGCAGA CAAGCAACCC TCACCTTGGC CCAGGGCCCA 
TCTTGCTGGG ATGATGTCTT AATTCCAAAC CGGATGAGTG GAGAGTGCCA ATCTCCAGAC 
TGCCCTGGGA CAAGAGCTGA ATTTTTCTTT AAATGTGGAG CACACCCAAC CTCAGACAAG 
GACACATCAG TAGCTTTGAA CCTGATCACC AACAACAGCC GCAGCATCCC CTGCATCGCG 
TGCACGGATG TCAGGAACCC TGTCTTGGTC TTCCAATGTA ACCACCGCCA CGTGATCTGT 
TTGGACTGCT TCCACTTGTA CTGTGTCACA AGGCTCAACG ATCGGCAGTT TGTCCACGAC 
GCTCAACTTG GCTACTCCCT GCCGTGTGTG GCTGGCTGTC CCAACTCCCT GATTAAAGAG 
CTCCATCACT TCAGGATCCT TGGAGAAGAG CAGTACAACA GGTACCAGCA GTATGGTGCC 
GAGGAGTGCG TGCTGCAGAT GGGAGGTGTG CTGTGCCCCC GTCCTGGCTG CGGAGCTGGG 
CTGCTGCCTG AACAGGGCCA GAGGAAAGTC ACCTGCGAAG GGGGCAACGG CCTGGGCTGT 
GGGTTCGTTT TCTGCCGGGA CTGCAAGGAA GCATACCATG AAGGGGAGTG CGACTCGATG 
TTCGAAGCCT CGGGGGCGAC TTCTCAGGCA TACCGGGTGG ATCAAAGAGC TGCTGAGCAA 
GCACGGTGGG AGGAGGCCTC CAAGGAAACC ATCAAGAAAA CCACCAAGCC TTGTCCTCGC 
TGCAATGTGC CCATTGAAAA GAATGGAGGA TGTATGCACA TGAAGTGTCC TCAGCCCCAG 
TGCAAGCTGG AGTGGTGCTG GAACTGTGGC TGTGAGTGGA ACCGAGCCTG CATGGGTGAT 
CACTGGTTTG ACGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064478.1
CDS	71..1468
Translation	MIVFVRFNSSYGFPVEVDSDTSIFQLKEVVAKRQGVPADQLRVIFAGKELQNHLTVQNCDLEQQSIVHIVQRPQRKSHETNASGGDKPQSTPEGSIWEPRSLTRVDLSSHILPADSVGLAVILDTDSKSDSEAARGPAAKPTYHSFFVYCKGPCHKVQPGKLRVQCGTCRQATLTLAQGPSCWDDVLIPNRMSGECQSPDCPGTRAEFFFKCGAHPTSDKDTSVALNLITNNSRSIPCIACTDVRNPVLVFQCNHRHVICLDCFHLYCVTRLNDRQFVHDAQLGYSLPCVAGCPNSLIKELHHFRILGEEQYNRYQQYGAEECVLQMGGVLCPRPGCGAGLLPEQGQRKVTCEGGNGLGCGFVFCRDCKEAYHEGECDSMFEASGATSQAYRVDQRAAEQARWEEASKETIKKTTKPCPRCNVPIEKNGGCMHMKCPQPQCKLEWCWNCGCEWNRACMGDHWFDV

Target ORF information:

RefSeq Version	NM_020093.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus parkin RBR E3 ubiquitin protein ligase (Prkn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020093.1

ORF Insert Sequence:

ATGATAGTGT TTGTCAGGTT CAACTCCAGC TATGGCTTCC CAGTGGAGGT CGATTCTGAC 
ACCAGCATCT TCCAGCTCAA GGAAGTGGTT GCTAAGCGAC AGGGGGTTCC AGCTGACCAG 
CTGCGAGTGA TTTTTGCTGG GAAGGAGCTG CAGAATCACC TGACAGTACA GAACTGTGAC 
CTGGAACAAC AGAGTATCGT TCACATAGTA CAGAGACCAC AGAGGAAAAG TCACGAAACA 
AATGCCTCTG GAGGGGACAA ACCCCAGAGC ACCCCGGAGG GCTCCATATG GGAGCCCAGA 
AGCTTGACTC GAGTGGACCT CAGCAGCCAT ATCCTGCCAG CGGACTCCGT GGGGCTGGCA 
GTCATTCTGG ACACAGACAG CAAGAGTGAC TCAGAAGCAG CCAGAGGTCC AGCAGCTAAA 
CCCACCTACC ACAGCTTTTT TGTCTACTGC AAAGGCCCCT GCCACAAGGT CCAGCCTGGG 
AAACTCCGAG TTCAGTGCGG CACCTGCAGA CAAGCAACCC TCACCTTGGC CCAGGGCCCA 
TCTTGCTGGG ATGATGTCTT AATTCCAAAC CGGATGAGTG GAGAGTGCCA ATCTCCAGAC 
TGCCCTGGGA CAAGAGCTGA ATTTTTCTTT AAATGTGGAG CACACCCAAC CTCAGACAAG 
GACACATCAG TAGCTTTGAA CCTGATCACC AACAACAGCC GCAGCATCCC CTGCATCGCG 
TGCACGGATG TCAGGAACCC TGTCTTGGTC TTCCAATGTA ACCACCGCCA CGTGATCTGT 
TTGGACTGCT TCCACTTGTA CTGTGTCACA AGGCTCAACG ATCGGCAGTT TGTCCACGAC 
GCTCAACTTG GCTACTCCCT GCCGTGTGTG GCTGGCTGTC CCAACTCCCT GATTAAAGAG 
CTCCATCACT TCAGGATCCT TGGAGAAGAG CAGTACAACA GGTACCAGCA GTATGGTGCC 
GAGGAGTGCG TGCTGCAGAT GGGAGGTGTG CTGTGCCCCC GTCCTGGCTG CGGAGCTGGG 
CTGCTGCCTG AACAGGGCCA GAGGAAAGTC ACCTGCGAAG GGGGCAACGG CCTGGGCTGT 
GGGTTCGTTT TCTGCCGGGA CTGCAAGGAA GCATACCATG AAGGGGAGTG CGACTCGATG 
TTCGAAGCCT CGGGGGCGAC TTCTCAGGCA TACCGGGTGG ATCAAAGAGC TGCTGAGCAA 
GCACGGTGGG AGGAGGCCTC CAAGGAAACC ATCAAGAAAA CCACCAAGCC TTGTCCTCGC 
TGCAATGTGC CCATTGAAAA GAATGGAGGA TGTATGCACA TGAAGTGTCC TCAGCCCCAG 
TGCAAGCTGG AGTGGTGCTG GAACTGTGGC TGTGAGTGGA ACCGAGCCTG CATGGGTGAT 
CACTGGTTTG ACGTGTAG