Pdp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pdp1
Entrez Gene ID	54705
Full Name	pyruvate dehyrogenase phosphatase catalytic subunit 1
Synonyms	Ppm2c
General protein information	Preferred Names pyruvate dehyrogenase phosphatase catalytic subunit 1 Names [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial pyruvate dehydrogenase [acetyl-transferring]-phosphatase 1, mitochondrial PDP 1 PDPC 1 protein phosphatase 2C, magnesium dependent, catalytic subunit pyruvate dehydrogenase phosphatase isoenzyme 1 pyruvate dehydrogenase phosphatase, catalytic subunit 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q13
Summary	catalytic subunit of the mitochondrial matrix phosphatase; involved in reactivation of the pyruvate dehydrogenase complex [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_019372.4	NP_062245.2	[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial isoform 2
NM_001271108.2	NP_001258037.1	[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial isoform 1
XM_006237907.3	XP_006237969.1	pyruvate dehydrogenase [acetyl-transferring]-phosphatase 1, mitochondrial isoform X1
XM_006237909.3	XP_006237971.1	pyruvate dehydrogenase [acetyl-transferring]-phosphatase 1, mitochondrial isoform X3
XM_006237908.2	XP_006237970.1	pyruvate dehydrogenase [acetyl-transferring]-phosphatase 1, mitochondrial isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Pdp1	NP_001258037.1
Danio rerio (zebrafish)	pdp1	NP_001104628.1
Mus musculus (house mouse)	Pdp1	NP_001091700.1
Pan troglodytes (chimpanzee)	PDP1	XP_003311894.1
	PDP1	XP_002805489.1
Bos taurus (cattle)	PDP1	NP_001193282.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008349	XP_317107.3
Homo sapiens (human)	PDP1	NP_001155251.1
Canis lupus familiaris (dog)	PDP1	XP_005638184.1
Caenorhabditis elegans (roundworm)	pdp-1	NP_491357.1
Xenopus tropicalis (tropical clawed frog)	pdp1	NP_001072271.1
Gallus gallus (chicken)	PDP1	NP_001186839.1
Drosophila melanogaster (fruit fly)	Pdp	NP_572404.1

Gene Ontology
Bibliography

Related articles in PubMed

Pyruvate dehydrogenase phosphatase1 mRNA expression is divergently and dynamically regulated between rat cerebral cortex, hippocampus and thalamus after traumatic brain injury: a potential biomarker of TBI-induced hyper- and hypo-glycaemia and neuronal vulnerability.
Xing G, Ren M, O'Neill JT, Sharma P, Verma A, Watson WD
Neuroscience letters525(2)140-5(2012 Sep)

Ppm1E is an in cellulo AMP-activated protein kinase phosphatase.
Voss M, Paterson J, Kelsall IR, Mart?n-Granados C, Hastie CJ, Peggie MW, Cohen PT
Cellular signalling23(1)114-24(2011 Jan)

Skeletal muscle type comparison of pyruvate dehydrogenase phosphatase activity and isoform expression: effects of obesity and endurance training.
Leblanc PJ, Mulligan M, Antolic A, Macpherson L, Inglis JG, Martin D, Roy BD, Peters SJ
American journal of physiology. Regulatory, integrative and comparative physiology295(4)R1224-30(2008 Oct)

Crystal structure of pyruvate dehydrogenase phosphatase 1 and its functional implications.
Vassylyev DG, Symersky J
Journal of molecular biology370(3)417-26(2007 Jul)

Pyruvate dehydrogenase kinase regulatory mechanisms and inhibition in treating diabetes, heart ischemia, and cancer.
Roche TE, Hiromasa Y
Cellular and molecular life sciences : CMLS64(7-8)830-49(2007 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

divergent alterations of PDP1 mRNA expression were found in different brain regions following traumatic brain injury.
Title: Pyruvate dehydrogenase phosphatase1 mRNA expression is divergently and dynamically regulated between rat cerebral cortex, hippocampus and thalamus after traumatic brain injury: a potential biomarker of TBI-induced hyper- and hypo-glycaemia and neuronal vulnerability.

Results suggest that decreased PDP activity in oxidative skeletal muscles may play a role in the impairment of carbohydrate metabolism in obese rats, which is reversible with endurance training.
Title: Skeletal muscle type comparison of pyruvate dehydrogenase phosphatase activity and isoform expression: effects of obesity and endurance training.

Results report the crystal structure of the rat pyruvate dehydrogenase phosphatase 1 catalytic subunit at 1.8 A resolution.
Title: Crystal structure of pyruvate dehydrogenase phosphatase 1 and its functional implications.

role of PP2C and AMPK in the derangements of cardiac lipid metabolism in obesity
Title: Role of PP2C in cardiac lipid accumulation in obese rodents and its prevention by troglitazone.

Asp445 might be involved in the control of interactions between PDP1c and the protein substrate pyruvate dehydrogenase
Title: Probing a putative active site of the catalytic subunit of pyruvate dehydrogenase phosphatase 1 (PDP1c) by site-directed mutagenesis.

The following Pdp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pdp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa13236	NM_019372.4 Latest version!	Rattus norvegicus pyruvate dehyrogenase phosphatase catalytic subunit 1 (Pdp1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa13318	NM_001271108.2 Latest version!	Rattus norvegicus pyruvate dehyrogenase phosphatase catalytic subunit 1 (Pdp1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa13318	XM_006237909.3 Latest version!	Rattus norvegicus pyruvate dehyrogenase phosphatase catalytic subunit 1 (Pdp1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa28055	XM_006237907.3 Latest version!	Rattus norvegicus pyruvate dehyrogenase phosphatase catalytic subunit 1 (Pdp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa28056	XM_006237908.2 Latest version!	Rattus norvegicus pyruvate dehyrogenase phosphatase catalytic subunit 1 (Pdp1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pdp1 ( NM_019372.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062245.2 Rattus norvegicus pyruvate dehyrogenase phosphatase catalytic subunit 1 (Pdp1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa13236
Clone ID Related Accession (Same CDS sequence)	NM_019372.4
Accession Version	NM_019372.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FN800686.1 and AABR07047259.1. On Sep 4, 2012 this sequence version replaced NM_019372.3. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream AUG, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: FN800686.1, AF062740.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGCCAGCAC CAACTCAACT GTTTTTTCCT CTCGTCCGTA ACTGTGAACT GAGCAGAATC 
TATGGCACTG CATGTTACTG CCACCACAAA CATCTCTGCT GTTCACCACC GTACATTCCT 
CAGAATCGTC TGAGATACAC ACCCCATCCA GCATATGCTA CCTTTTGTAG GCCAAGGGAG 
AACTGGTGGC AGTATACTCA AGGAAGGAGA TATGCTTCTA CGCCTCAGAA ATTTTACCTC 
ACACCTCCAC AAGTCAACAG CATCCTTAAA GCTAATGAAT ACAGCTTCAA AGTACCAGAA 
TTTGATGGCA AAAATGTCAG TTCCATTCTT GGATTTGACA GCAATCAGCT GCCTGCAAAT 
GCACCCATAG AAGACCGGAG AAGTGCAGCA ACCTGCTTGC AGACCAGAGG GATGCTTTTG 
GGCGTTTTTG ATGGTCATGC AGGCTGTGCT TGCTCCCAGG CAGTCAGTGA AAGACTCTTC 
TATTATATTG CTGTTTCCTT GTTACCCCAT GAGACTTTGC TAGAGATTGA AAATGCAGTG 
GAGAGTGGTC GGGCACTGCT ACCTATCCTT CAGTGGCACA AGCACCCCAA TGATTACTTC 
AGTAAGGAGG CATCCAAATT GTATTTCAAC AGCTTGAGGA CTTACTGGCA AGAGCTTATA 
GACCTCAATA CTGGAGAATC AGCTGATATT GATGTTAAGG AGGCTTTAAT TAATGCTTTC 
AAGAGACTTG ATAATGACAT TTCATTGGAG GCTCAAGTCG GTGATCCTAA TTCTTTTCTC 
AATTACCTGG TGCTTCGGGT AGCATTTTCT GGGGCTACTG CTTGTGTGGC CCATGTAGAT 
GGTGTTGACC TCCATGTGGC TAACACTGGC GATAGTAGAG CCATGCTAGG TGTGCAAGAA 
GAAGATGGCT CCTGGTCAGC AGTCACGCTC TCTAATGACC ACAATGCTCA GAATGAAAGA 
GAACTAGAAC GTCTAAAACT GGAACACCCA AAAAATGAGG CCAAGAGCGT GGTAAAGCAG 
GATCGGCTGC TTGGCTTGCT GATGCCCTTT AGGGCTTTTG GGGATGTAAA GTTCAAATGG 
AGCATTGACC TTCAAAAGAG AGTGATAGAG TCTGGCCCAG ACCAGTTGAA TGACAATGAA 
TACACCAAGT TTATCCCTCC TAACTATCAT ACACCTCCTT ATCTCACTGC TGAGCCAGAG 
GTAACTTATC ACCGATTAAG GCCACAGGAT AAATTCCTAG TGTTAGCAAC TGATGGATTG 
TGGGAGACTA TGCATAGACA GGATGTGGTT AGGATTGTGG GTGAGTACTT AACTGGTATG 
CATCACCAAC AGCCAATAGC TGTTGGTGGG TACAAGGTGA CTCTGGGACA GATGCATGGC 
CTTTTAACAG AAAGGAGAGC AAAGATGTCA TCAGTCTTTG AGGATCAGAA TGCAGCAACC 
CATCTCATTC GCCATGCTGT AGGCAATAAT GAATTTGGAG CTGTTGATCA TGAACGACTC 
TCTAAAATGC TTAGCCTTCC TGAAGAGCTT GCTCGGATGT ATAGAGATGA CATTACAATC 
ATTGTAGTTC AGTTCAATTC TCATGTTGTA GGAGCATATC AAAACCAGGA ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062245.2
CDS	145..1761
Translation	MPAPTQLFFPLVRNCELSRIYGTACYCHHKHLCCSPPYIPQNRLRYTPHPAYATFCRPRENWWQYTQGRRYASTPQKFYLTPPQVNSILKANEYSFKVPEFDGKNVSSILGFDSNQLPANAPIEDRRSAATCLQTRGMLLGVFDGHAGCACSQAVSERLFYYIAVSLLPHETLLEIENAVESGRALLPILQWHKHPNDYFSKEASKLYFNSLRTYWQELIDLNTGESADIDVKEALINAFKRLDNDISLEAQVGDPNSFLNYLVLRVAFSGATACVAHVDGVDLHVANTGDSRAMLGVQEEDGSWSAVTLSNDHNAQNERELERLKLEHPKNEAKSVVKQDRLLGLLMPFRAFGDVKFKWSIDLQKRVIESGPDQLNDNEYTKFIPPNYHTPPYLTAEPEVTYHRLRPQDKFLVLATDGLWETMHRQDVVRIVGEYLTGMHHQQPIAVGGYKVTLGQMHGLLTERRAKMSSVFEDQNAATHLIRHAVGNNEFGAVDHERLSKMLSLPEELARMYRDDITIIVVQFNSHVVGAYQNQEQ

Target ORF information:

RefSeq Version	NM_019372.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pyruvate dehyrogenase phosphatase catalytic subunit 1 (Pdp1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019372.4

ORF Insert Sequence:

ATGCCAGCAC CAACTCAACT GTTTTTTCCT CTCGTCCGTA ACTGTGAACT GAGCAGAATC 
TATGGCACTG CATGTTACTG CCACCACAAA CATCTCTGCT GTTCACCACC GTACATTCCT 
CAGAATCGTC TGAGATACAC ACCCCATCCA GCATATGCTA CCTTTTGTAG GCCAAGGGAG 
AACTGGTGGC AGTATACTCA AGGAAGGAGA TATGCTTCTA CGCCTCAGAA ATTTTACCTC 
ACACCTCCAC AAGTCAACAG CATCCTTAAA GCTAATGAAT ACAGCTTCAA AGTACCAGAA 
TTTGATGGCA AAAATGTCAG TTCCATTCTT GGATTTGACA GCAATCAGCT GCCTGCAAAT 
GCACCCATAG AAGACCGGAG AAGTGCAGCA ACCTGCTTGC AGACCAGAGG GATGCTTTTG 
GGCGTTTTTG ATGGTCATGC AGGCTGTGCT TGCTCCCAGG CAGTCAGTGA AAGACTCTTC 
TATTATATTG CTGTTTCCTT GTTACCCCAT GAGACTTTGC TAGAGATTGA AAATGCAGTG 
GAGAGTGGTC GGGCACTGCT ACCTATCCTT CAGTGGCACA AGCACCCCAA TGATTACTTC 
AGTAAGGAGG CATCCAAATT GTATTTCAAC AGCTTGAGGA CTTACTGGCA AGAGCTTATA 
GACCTCAATA CTGGAGAATC AGCTGATATT GATGTTAAGG AGGCTTTAAT TAATGCTTTC 
AAGAGACTTG ATAATGACAT TTCATTGGAG GCTCAAGTCG GTGATCCTAA TTCTTTTCTC 
AATTACCTGG TGCTTCGGGT AGCATTTTCT GGGGCTACTG CTTGTGTGGC CCATGTAGAT 
GGTGTTGACC TCCATGTGGC TAACACTGGC GATAGTAGAG CCATGCTAGG TGTGCAAGAA 
GAAGATGGCT CCTGGTCAGC AGTCACGCTC TCTAATGACC ACAATGCTCA GAATGAAAGA 
GAACTAGAAC GTCTAAAACT GGAACACCCA AAAAATGAGG CCAAGAGCGT GGTAAAGCAG 
GATCGGCTGC TTGGCTTGCT GATGCCCTTT AGGGCTTTTG GGGATGTAAA GTTCAAATGG 
AGCATTGACC TTCAAAAGAG AGTGATAGAG TCTGGCCCAG ACCAGTTGAA TGACAATGAA 
TACACCAAGT TTATCCCTCC TAACTATCAT ACACCTCCTT ATCTCACTGC TGAGCCAGAG 
GTAACTTATC ACCGATTAAG GCCACAGGAT AAATTCCTAG TGTTAGCAAC TGATGGATTG 
TGGGAGACTA TGCATAGACA GGATGTGGTT AGGATTGTGG GTGAGTACTT AACTGGTATG 
CATCACCAAC AGCCAATAGC TGTTGGTGGG TACAAGGTGA CTCTGGGACA GATGCATGGC 
CTTTTAACAG AAAGGAGAGC AAAGATGTCA TCAGTCTTTG AGGATCAGAA TGCAGCAACC 
CATCTCATTC GCCATGCTGT AGGCAATAAT GAATTTGGAG CTGTTGATCA TGAACGACTC 
TCTAAAATGC TTAGCCTTCC TGAAGAGCTT GCTCGGATGT ATAGAGATGA CATTACAATC 
ATTGTAGTTC AGTTCAATTC TCATGTTGTA GGAGCATATC AAAACCAGGA ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pdp1 ( NM_001271108.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001258037.1 Rattus norvegicus pyruvate dehyrogenase phosphatase catalytic subunit 1 (Pdp1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa13318
Clone ID Related Accession (Same CDS sequence)	NM_001271108.2 , XM_006237909.3
Accession Version	NM_001271108.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07047259.1. On Sep 4, 2012 this sequence version replaced NM_001271108.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AW520836.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTGTGTGT GTCCCGGGCC CAGACGAATT GGAATCCCGG TCAGAACATC CAGTCTACCA 
CTGTTCTCTG ATGCCATGCC AGCACCAACT CAACTGTTTT TTCCTCTCGT CCGTAACTGT 
GAACTGAGCA GAATCTATGG CACTGCATGT TACTGCCACC ACAAACATCT CTGCTGTTCA 
CCACCGTACA TTCCTCAGAA TCGTCTGAGA TACACACCCC ATCCAGCATA TGCTACCTTT 
TGTAGGCCAA GGGAGAACTG GTGGCAGTAT ACTCAAGGAA GGAGATATGC TTCTACGCCT 
CAGAAATTTT ACCTCACACC TCCACAAGTC AACAGCATCC TTAAAGCTAA TGAATACAGC 
TTCAAAGTAC CAGAATTTGA TGGCAAAAAT GTCAGTTCCA TTCTTGGATT TGACAGCAAT 
CAGCTGCCTG CAAATGCACC CATAGAAGAC CGGAGAAGTG CAGCAACCTG CTTGCAGACC 
AGAGGGATGC TTTTGGGCGT TTTTGATGGT CATGCAGGCT GTGCTTGCTC CCAGGCAGTC 
AGTGAAAGAC TCTTCTATTA TATTGCTGTT TCCTTGTTAC CCCATGAGAC TTTGCTAGAG 
ATTGAAAATG CAGTGGAGAG TGGTCGGGCA CTGCTACCTA TCCTTCAGTG GCACAAGCAC 
CCCAATGATT ACTTCAGTAA GGAGGCATCC AAATTGTATT TCAACAGCTT GAGGACTTAC 
TGGCAAGAGC TTATAGACCT CAATACTGGA GAATCAGCTG ATATTGATGT TAAGGAGGCT 
TTAATTAATG CTTTCAAGAG ACTTGATAAT GACATTTCAT TGGAGGCTCA AGTCGGTGAT 
CCTAATTCTT TTCTCAATTA CCTGGTGCTT CGGGTAGCAT TTTCTGGGGC TACTGCTTGT 
GTGGCCCATG TAGATGGTGT TGACCTCCAT GTGGCTAACA CTGGCGATAG TAGAGCCATG 
CTAGGTGTGC AAGAAGAAGA TGGCTCCTGG TCAGCAGTCA CGCTCTCTAA TGACCACAAT 
GCTCAGAATG AAAGAGAACT AGAACGTCTA AAACTGGAAC ACCCAAAAAA TGAGGCCAAG 
AGCGTGGTAA AGCAGGATCG GCTGCTTGGC TTGCTGATGC CCTTTAGGGC TTTTGGGGAT 
GTAAAGTTCA AATGGAGCAT TGACCTTCAA AAGAGAGTGA TAGAGTCTGG CCCAGACCAG 
TTGAATGACA ATGAATACAC CAAGTTTATC CCTCCTAACT ATCATACACC TCCTTATCTC 
ACTGCTGAGC CAGAGGTAAC TTATCACCGA TTAAGGCCAC AGGATAAATT CCTAGTGTTA 
GCAACTGATG GATTGTGGGA GACTATGCAT AGACAGGATG TGGTTAGGAT TGTGGGTGAG 
TACTTAACTG GTATGCATCA CCAACAGCCA ATAGCTGTTG GTGGGTACAA GGTGACTCTG 
GGACAGATGC ATGGCCTTTT AACAGAAAGG AGAGCAAAGA TGTCATCAGT CTTTGAGGAT 
CAGAATGCAG CAACCCATCT CATTCGCCAT GCTGTAGGCA ATAATGAATT TGGAGCTGTT 
GATCATGAAC GACTCTCTAA AATGCTTAGC CTTCCTGAAG AGCTTGCTCG GATGTATAGA 
GATGACATTA CAATCATTGT AGTTCAGTTC AATTCTCATG TTGTAGGAGC ATATCAAAAC 
CAGGAACAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258037.1
CDS	75..1766
Translation	MCVCPGPRRIGIPVRTSSLPLFSDAMPAPTQLFFPLVRNCELSRIYGTACYCHHKHLCCSPPYIPQNRLRYTPHPAYATFCRPRENWWQYTQGRRYASTPQKFYLTPPQVNSILKANEYSFKVPEFDGKNVSSILGFDSNQLPANAPIEDRRSAATCLQTRGMLLGVFDGHAGCACSQAVSERLFYYIAVSLLPHETLLEIENAVESGRALLPILQWHKHPNDYFSKEASKLYFNSLRTYWQELIDLNTGESADIDVKEALINAFKRLDNDISLEAQVGDPNSFLNYLVLRVAFSGATACVAHVDGVDLHVANTGDSRAMLGVQEEDGSWSAVTLSNDHNAQNERELERLKLEHPKNEAKSVVKQDRLLGLLMPFRAFGDVKFKWSIDLQKRVIESGPDQLNDNEYTKFIPPNYHTPPYLTAEPEVTYHRLRPQDKFLVLATDGLWETMHRQDVVRIVGEYLTGMHHQQPIAVGGYKVTLGQMHGLLTERRAKMSSVFEDQNAATHLIRHAVGNNEFGAVDHERLSKMLSLPEELARMYRDDITIIVVQFNSHVVGAYQNQEQ

Target ORF information:

RefSeq Version	NM_001271108.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pyruvate dehyrogenase phosphatase catalytic subunit 1 (Pdp1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271108.2

ORF Insert Sequence:

ATGTGTGTGT GTCCCGGGCC CAGACGAATT GGAATCCCGG TCAGAACATC CAGTCTACCA 
CTGTTCTCTG ATGCCATGCC AGCACCAACT CAACTGTTTT TTCCTCTCGT CCGTAACTGT 
GAACTGAGCA GAATCTATGG CACTGCATGT TACTGCCACC ACAAACATCT CTGCTGTTCA 
CCACCGTACA TTCCTCAGAA TCGTCTGAGA TACACACCCC ATCCAGCATA TGCTACCTTT 
TGTAGGCCAA GGGAGAACTG GTGGCAGTAT ACTCAAGGAA GGAGATATGC TTCTACGCCT 
CAGAAATTTT ACCTCACACC TCCACAAGTC AACAGCATCC TTAAAGCTAA TGAATACAGC 
TTCAAAGTAC CAGAATTTGA TGGCAAAAAT GTCAGTTCCA TTCTTGGATT TGACAGCAAT 
CAGCTGCCTG CAAATGCACC CATAGAAGAC CGGAGAAGTG CAGCAACCTG CTTGCAGACC 
AGAGGGATGC TTTTGGGCGT TTTTGATGGT CATGCAGGCT GTGCTTGCTC CCAGGCAGTC 
AGTGAAAGAC TCTTCTATTA TATTGCTGTT TCCTTGTTAC CCCATGAGAC TTTGCTAGAG 
ATTGAAAATG CAGTGGAGAG TGGTCGGGCA CTGCTACCTA TCCTTCAGTG GCACAAGCAC 
CCCAATGATT ACTTCAGTAA GGAGGCATCC AAATTGTATT TCAACAGCTT GAGGACTTAC 
TGGCAAGAGC TTATAGACCT CAATACTGGA GAATCAGCTG ATATTGATGT TAAGGAGGCT 
TTAATTAATG CTTTCAAGAG ACTTGATAAT GACATTTCAT TGGAGGCTCA AGTCGGTGAT 
CCTAATTCTT TTCTCAATTA CCTGGTGCTT CGGGTAGCAT TTTCTGGGGC TACTGCTTGT 
GTGGCCCATG TAGATGGTGT TGACCTCCAT GTGGCTAACA CTGGCGATAG TAGAGCCATG 
CTAGGTGTGC AAGAAGAAGA TGGCTCCTGG TCAGCAGTCA CGCTCTCTAA TGACCACAAT 
GCTCAGAATG AAAGAGAACT AGAACGTCTA AAACTGGAAC ACCCAAAAAA TGAGGCCAAG 
AGCGTGGTAA AGCAGGATCG GCTGCTTGGC TTGCTGATGC CCTTTAGGGC TTTTGGGGAT 
GTAAAGTTCA AATGGAGCAT TGACCTTCAA AAGAGAGTGA TAGAGTCTGG CCCAGACCAG 
TTGAATGACA ATGAATACAC CAAGTTTATC CCTCCTAACT ATCATACACC TCCTTATCTC 
ACTGCTGAGC CAGAGGTAAC TTATCACCGA TTAAGGCCAC AGGATAAATT CCTAGTGTTA 
GCAACTGATG GATTGTGGGA GACTATGCAT AGACAGGATG TGGTTAGGAT TGTGGGTGAG 
TACTTAACTG GTATGCATCA CCAACAGCCA ATAGCTGTTG GTGGGTACAA GGTGACTCTG 
GGACAGATGC ATGGCCTTTT AACAGAAAGG AGAGCAAAGA TGTCATCAGT CTTTGAGGAT 
CAGAATGCAG CAACCCATCT CATTCGCCAT GCTGTAGGCA ATAATGAATT TGGAGCTGTT 
GATCATGAAC GACTCTCTAA AATGCTTAGC CTTCCTGAAG AGCTTGCTCG GATGTATAGA 
GATGACATTA CAATCATTGT AGTTCAGTTC AATTCTCATG TTGTAGGAGC ATATCAAAAC 
CAGGAACAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pdp1 ( XM_006237909.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006237971.1 Rattus norvegicus pyruvate dehyrogenase phosphatase catalytic subunit 1 (Pdp1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa13318
Clone ID Related Accession (Same CDS sequence)	NM_001271108.2 , XM_006237909.3
Accession Version	XM_006237909.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	pyruvate dehydrogenase [acetyl-transferring]-phosphatase 1, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006237909.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGTGT GTCCCGGGCC CAGACGAATT GGAATCCCGG TCAGAACATC CAGTCTACCA 
CTGTTCTCTG ATGCCATGCC AGCACCAACT CAACTGTTTT TTCCTCTCGT CCGTAACTGT 
GAACTGAGCA GAATCTATGG CACTGCATGT TACTGCCACC ACAAACATCT CTGCTGTTCA 
CCACCGTACA TTCCTCAGAA TCGTCTGAGA TACACACCCC ATCCAGCATA TGCTACCTTT 
TGTAGGCCAA GGGAGAACTG GTGGCAGTAT ACTCAAGGAA GGAGATATGC TTCTACGCCT 
CAGAAATTTT ACCTCACACC TCCACAAGTC AACAGCATCC TTAAAGCTAA TGAATACAGC 
TTCAAAGTAC CAGAATTTGA TGGCAAAAAT GTCAGTTCCA TTCTTGGATT TGACAGCAAT 
CAGCTGCCTG CAAATGCACC CATAGAAGAC CGGAGAAGTG CAGCAACCTG CTTGCAGACC 
AGAGGGATGC TTTTGGGCGT TTTTGATGGT CATGCAGGCT GTGCTTGCTC CCAGGCAGTC 
AGTGAAAGAC TCTTCTATTA TATTGCTGTT TCCTTGTTAC CCCATGAGAC TTTGCTAGAG 
ATTGAAAATG CAGTGGAGAG TGGTCGGGCA CTGCTACCTA TCCTTCAGTG GCACAAGCAC 
CCCAATGATT ACTTCAGTAA GGAGGCATCC AAATTGTATT TCAACAGCTT GAGGACTTAC 
TGGCAAGAGC TTATAGACCT CAATACTGGA GAATCAGCTG ATATTGATGT TAAGGAGGCT 
TTAATTAATG CTTTCAAGAG ACTTGATAAT GACATTTCAT TGGAGGCTCA AGTCGGTGAT 
CCTAATTCTT TTCTCAATTA CCTGGTGCTT CGGGTAGCAT TTTCTGGGGC TACTGCTTGT 
GTGGCCCATG TAGATGGTGT TGACCTCCAT GTGGCTAACA CTGGCGATAG TAGAGCCATG 
CTAGGTGTGC AAGAAGAAGA TGGCTCCTGG TCAGCAGTCA CGCTCTCTAA TGACCACAAT 
GCTCAGAATG AAAGAGAACT AGAACGTCTA AAACTGGAAC ACCCAAAAAA TGAGGCCAAG 
AGCGTGGTAA AGCAGGATCG GCTGCTTGGC TTGCTGATGC CCTTTAGGGC TTTTGGGGAT 
GTAAAGTTCA AATGGAGCAT TGACCTTCAA AAGAGAGTGA TAGAGTCTGG CCCAGACCAG 
TTGAATGACA ATGAATACAC CAAGTTTATC CCTCCTAACT ATCATACACC TCCTTATCTC 
ACTGCTGAGC CAGAGGTAAC TTATCACCGA TTAAGGCCAC AGGATAAATT CCTAGTGTTA 
GCAACTGATG GATTGTGGGA GACTATGCAT AGACAGGATG TGGTTAGGAT TGTGGGTGAG 
TACTTAACTG GTATGCATCA CCAACAGCCA ATAGCTGTTG GTGGGTACAA GGTGACTCTG 
GGACAGATGC ATGGCCTTTT AACAGAAAGG AGAGCAAAGA TGTCATCAGT CTTTGAGGAT 
CAGAATGCAG CAACCCATCT CATTCGCCAT GCTGTAGGCA ATAATGAATT TGGAGCTGTT 
GATCATGAAC GACTCTCTAA AATGCTTAGC CTTCCTGAAG AGCTTGCTCG GATGTATAGA 
GATGACATTA CAATCATTGT AGTTCAGTTC AATTCTCATG TTGTAGGAGC ATATCAAAAC 
CAGGAACAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006237971.1
CDS	152..1843
Translation	MCVCPGPRRIGIPVRTSSLPLFSDAMPAPTQLFFPLVRNCELSRIYGTACYCHHKHLCCSPPYIPQNRLRYTPHPAYATFCRPRENWWQYTQGRRYASTPQKFYLTPPQVNSILKANEYSFKVPEFDGKNVSSILGFDSNQLPANAPIEDRRSAATCLQTRGMLLGVFDGHAGCACSQAVSERLFYYIAVSLLPHETLLEIENAVESGRALLPILQWHKHPNDYFSKEASKLYFNSLRTYWQELIDLNTGESADIDVKEALINAFKRLDNDISLEAQVGDPNSFLNYLVLRVAFSGATACVAHVDGVDLHVANTGDSRAMLGVQEEDGSWSAVTLSNDHNAQNERELERLKLEHPKNEAKSVVKQDRLLGLLMPFRAFGDVKFKWSIDLQKRVIESGPDQLNDNEYTKFIPPNYHTPPYLTAEPEVTYHRLRPQDKFLVLATDGLWETMHRQDVVRIVGEYLTGMHHQQPIAVGGYKVTLGQMHGLLTERRAKMSSVFEDQNAATHLIRHAVGNNEFGAVDHERLSKMLSLPEELARMYRDDITIIVVQFNSHVVGAYQNQEQ

Target ORF information:

RefSeq Version	XM_006237909.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pyruvate dehyrogenase phosphatase catalytic subunit 1 (Pdp1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006237909.3

ORF Insert Sequence:

ATGTGTGTGT GTCCCGGGCC CAGACGAATT GGAATCCCGG TCAGAACATC CAGTCTACCA 
CTGTTCTCTG ATGCCATGCC AGCACCAACT CAACTGTTTT TTCCTCTCGT CCGTAACTGT 
GAACTGAGCA GAATCTATGG CACTGCATGT TACTGCCACC ACAAACATCT CTGCTGTTCA 
CCACCGTACA TTCCTCAGAA TCGTCTGAGA TACACACCCC ATCCAGCATA TGCTACCTTT 
TGTAGGCCAA GGGAGAACTG GTGGCAGTAT ACTCAAGGAA GGAGATATGC TTCTACGCCT 
CAGAAATTTT ACCTCACACC TCCACAAGTC AACAGCATCC TTAAAGCTAA TGAATACAGC 
TTCAAAGTAC CAGAATTTGA TGGCAAAAAT GTCAGTTCCA TTCTTGGATT TGACAGCAAT 
CAGCTGCCTG CAAATGCACC CATAGAAGAC CGGAGAAGTG CAGCAACCTG CTTGCAGACC 
AGAGGGATGC TTTTGGGCGT TTTTGATGGT CATGCAGGCT GTGCTTGCTC CCAGGCAGTC 
AGTGAAAGAC TCTTCTATTA TATTGCTGTT TCCTTGTTAC CCCATGAGAC TTTGCTAGAG 
ATTGAAAATG CAGTGGAGAG TGGTCGGGCA CTGCTACCTA TCCTTCAGTG GCACAAGCAC 
CCCAATGATT ACTTCAGTAA GGAGGCATCC AAATTGTATT TCAACAGCTT GAGGACTTAC 
TGGCAAGAGC TTATAGACCT CAATACTGGA GAATCAGCTG ATATTGATGT TAAGGAGGCT 
TTAATTAATG CTTTCAAGAG ACTTGATAAT GACATTTCAT TGGAGGCTCA AGTCGGTGAT 
CCTAATTCTT TTCTCAATTA CCTGGTGCTT CGGGTAGCAT TTTCTGGGGC TACTGCTTGT 
GTGGCCCATG TAGATGGTGT TGACCTCCAT GTGGCTAACA CTGGCGATAG TAGAGCCATG 
CTAGGTGTGC AAGAAGAAGA TGGCTCCTGG TCAGCAGTCA CGCTCTCTAA TGACCACAAT 
GCTCAGAATG AAAGAGAACT AGAACGTCTA AAACTGGAAC ACCCAAAAAA TGAGGCCAAG 
AGCGTGGTAA AGCAGGATCG GCTGCTTGGC TTGCTGATGC CCTTTAGGGC TTTTGGGGAT 
GTAAAGTTCA AATGGAGCAT TGACCTTCAA AAGAGAGTGA TAGAGTCTGG CCCAGACCAG 
TTGAATGACA ATGAATACAC CAAGTTTATC CCTCCTAACT ATCATACACC TCCTTATCTC 
ACTGCTGAGC CAGAGGTAAC TTATCACCGA TTAAGGCCAC AGGATAAATT CCTAGTGTTA 
GCAACTGATG GATTGTGGGA GACTATGCAT AGACAGGATG TGGTTAGGAT TGTGGGTGAG 
TACTTAACTG GTATGCATCA CCAACAGCCA ATAGCTGTTG GTGGGTACAA GGTGACTCTG 
GGACAGATGC ATGGCCTTTT AACAGAAAGG AGAGCAAAGA TGTCATCAGT CTTTGAGGAT 
CAGAATGCAG CAACCCATCT CATTCGCCAT GCTGTAGGCA ATAATGAATT TGGAGCTGTT 
GATCATGAAC GACTCTCTAA AATGCTTAGC CTTCCTGAAG AGCTTGCTCG GATGTATAGA 
GATGACATTA CAATCATTGT AGTTCAGTTC AATTCTCATG TTGTAGGAGC ATATCAAAAC 
CAGGAACAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pdp1 ( XM_006237907.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006237969.1 Rattus norvegicus pyruvate dehyrogenase phosphatase catalytic subunit 1 (Pdp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa28055
Clone ID Related Accession (Same CDS sequence)	XM_006237907.3
Accession Version	XM_006237907.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	pyruvate dehydrogenase [acetyl-transferring]-phosphatase 1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006237907.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATCT CTGCCTTGCT TTCAATGGGC CGCTGCTGCT GTCGCTGCTG CTGTCCGCGC 
GGGTTGTGGA TGTTGTCGGC TCCGTGTTGT GATGACAGGA GAATGTGTGT GTGTCCCGGG 
CCCAGACGAA TTGGAATCCC GGTCAGAACA TCCAGTCTAC CACTGTTCTC TGATGCCATG 
CCAGCACCAA CTCAACTGTT TTTTCCTCTC GTCCGTAACT GTGAACTGAG CAGAATCTAT 
GGCACTGCAT GTTACTGCCA CCACAAACAT CTCTGCTGTT CACCACCGTA CATTCCTCAG 
AATCGTCTGA GATACACACC CCATCCAGCA TATGCTACCT TTTGTAGGCC AAGGGAGAAC 
TGGTGGCAGT ATACTCAAGG AAGGAGATAT GCTTCTACGC CTCAGAAATT TTACCTCACA 
CCTCCACAAG TCAACAGCAT CCTTAAAGCT AATGAATACA GCTTCAAAGT ACCAGAATTT 
GATGGCAAAA ATGTCAGTTC CATTCTTGGA TTTGACAGCA ATCAGCTGCC TGCAAATGCA 
CCCATAGAAG ACCGGAGAAG TGCAGCAACC TGCTTGCAGA CCAGAGGGAT GCTTTTGGGC 
GTTTTTGATG GTCATGCAGG CTGTGCTTGC TCCCAGGCAG TCAGTGAAAG ACTCTTCTAT 
TATATTGCTG TTTCCTTGTT ACCCCATGAG ACTTTGCTAG AGATTGAAAA TGCAGTGGAG 
AGTGGTCGGG CACTGCTACC TATCCTTCAG TGGCACAAGC ACCCCAATGA TTACTTCAGT 
AAGGAGGCAT CCAAATTGTA TTTCAACAGC TTGAGGACTT ACTGGCAAGA GCTTATAGAC 
CTCAATACTG GAGAATCAGC TGATATTGAT GTTAAGGAGG CTTTAATTAA TGCTTTCAAG 
AGACTTGATA ATGACATTTC ATTGGAGGCT CAAGTCGGTG ATCCTAATTC TTTTCTCAAT 
TACCTGGTGC TTCGGGTAGC ATTTTCTGGG GCTACTGCTT GTGTGGCCCA TGTAGATGGT 
GTTGACCTCC ATGTGGCTAA CACTGGCGAT AGTAGAGCCA TGCTAGGTGT GCAAGAAGAA 
GATGGCTCCT GGTCAGCAGT CACGCTCTCT AATGACCACA ATGCTCAGAA TGAAAGAGAA 
CTAGAACGTC TAAAACTGGA ACACCCAAAA AATGAGGCCA AGAGCGTGGT AAAGCAGGAT 
CGGCTGCTTG GCTTGCTGAT GCCCTTTAGG GCTTTTGGGG ATGTAAAGTT CAAATGGAGC 
ATTGACCTTC AAAAGAGAGT GATAGAGTCT GGCCCAGACC AGTTGAATGA CAATGAATAC 
ACCAAGTTTA TCCCTCCTAA CTATCATACA CCTCCTTATC TCACTGCTGA GCCAGAGGTA 
ACTTATCACC GATTAAGGCC ACAGGATAAA TTCCTAGTGT TAGCAACTGA TGGATTGTGG 
GAGACTATGC ATAGACAGGA TGTGGTTAGG ATTGTGGGTG AGTACTTAAC TGGTATGCAT 
CACCAACAGC CAATAGCTGT TGGTGGGTAC AAGGTGACTC TGGGACAGAT GCATGGCCTT 
TTAACAGAAA GGAGAGCAAA GATGTCATCA GTCTTTGAGG ATCAGAATGC AGCAACCCAT 
CTCATTCGCC ATGCTGTAGG CAATAATGAA TTTGGAGCTG TTGATCATGA ACGACTCTCT 
AAAATGCTTA GCCTTCCTGA AGAGCTTGCT CGGATGTATA GAGATGACAT TACAATCATT 
GTAGTTCAGT TCAATTCTCA TGTTGTAGGA GCATATCAAA ACCAGGAACA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006237969.1
CDS	89..1882
Translation	MSISALLSMGRCCCRCCCPRGLWMLSAPCCDDRRMCVCPGPRRIGIPVRTSSLPLFSDAMPAPTQLFFPLVRNCELSRIYGTACYCHHKHLCCSPPYIPQNRLRYTPHPAYATFCRPRENWWQYTQGRRYASTPQKFYLTPPQVNSILKANEYSFKVPEFDGKNVSSILGFDSNQLPANAPIEDRRSAATCLQTRGMLLGVFDGHAGCACSQAVSERLFYYIAVSLLPHETLLEIENAVESGRALLPILQWHKHPNDYFSKEASKLYFNSLRTYWQELIDLNTGESADIDVKEALINAFKRLDNDISLEAQVGDPNSFLNYLVLRVAFSGATACVAHVDGVDLHVANTGDSRAMLGVQEEDGSWSAVTLSNDHNAQNERELERLKLEHPKNEAKSVVKQDRLLGLLMPFRAFGDVKFKWSIDLQKRVIESGPDQLNDNEYTKFIPPNYHTPPYLTAEPEVTYHRLRPQDKFLVLATDGLWETMHRQDVVRIVGEYLTGMHHQQPIAVGGYKVTLGQMHGLLTERRAKMSSVFEDQNAATHLIRHAVGNNEFGAVDHERLSKMLSLPEELARMYRDDITIIVVQFNSHVVGAYQNQEQ

Target ORF information:

RefSeq Version	XM_006237907.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pyruvate dehyrogenase phosphatase catalytic subunit 1 (Pdp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006237907.3

ORF Insert Sequence:

ATGAGCATCT CTGCCTTGCT TTCAATGGGC CGCTGCTGCT GTCGCTGCTG CTGTCCGCGC 
GGGTTGTGGA TGTTGTCGGC TCCGTGTTGT GATGACAGGA GAATGTGTGT GTGTCCCGGG 
CCCAGACGAA TTGGAATCCC GGTCAGAACA TCCAGTCTAC CACTGTTCTC TGATGCCATG 
CCAGCACCAA CTCAACTGTT TTTTCCTCTC GTCCGTAACT GTGAACTGAG CAGAATCTAT 
GGCACTGCAT GTTACTGCCA CCACAAACAT CTCTGCTGTT CACCACCGTA CATTCCTCAG 
AATCGTCTGA GATACACACC CCATCCAGCA TATGCTACCT TTTGTAGGCC AAGGGAGAAC 
TGGTGGCAGT ATACTCAAGG AAGGAGATAT GCTTCTACGC CTCAGAAATT TTACCTCACA 
CCTCCACAAG TCAACAGCAT CCTTAAAGCT AATGAATACA GCTTCAAAGT ACCAGAATTT 
GATGGCAAAA ATGTCAGTTC CATTCTTGGA TTTGACAGCA ATCAGCTGCC TGCAAATGCA 
CCCATAGAAG ACCGGAGAAG TGCAGCAACC TGCTTGCAGA CCAGAGGGAT GCTTTTGGGC 
GTTTTTGATG GTCATGCAGG CTGTGCTTGC TCCCAGGCAG TCAGTGAAAG ACTCTTCTAT 
TATATTGCTG TTTCCTTGTT ACCCCATGAG ACTTTGCTAG AGATTGAAAA TGCAGTGGAG 
AGTGGTCGGG CACTGCTACC TATCCTTCAG TGGCACAAGC ACCCCAATGA TTACTTCAGT 
AAGGAGGCAT CCAAATTGTA TTTCAACAGC TTGAGGACTT ACTGGCAAGA GCTTATAGAC 
CTCAATACTG GAGAATCAGC TGATATTGAT GTTAAGGAGG CTTTAATTAA TGCTTTCAAG 
AGACTTGATA ATGACATTTC ATTGGAGGCT CAAGTCGGTG ATCCTAATTC TTTTCTCAAT 
TACCTGGTGC TTCGGGTAGC ATTTTCTGGG GCTACTGCTT GTGTGGCCCA TGTAGATGGT 
GTTGACCTCC ATGTGGCTAA CACTGGCGAT AGTAGAGCCA TGCTAGGTGT GCAAGAAGAA 
GATGGCTCCT GGTCAGCAGT CACGCTCTCT AATGACCACA ATGCTCAGAA TGAAAGAGAA 
CTAGAACGTC TAAAACTGGA ACACCCAAAA AATGAGGCCA AGAGCGTGGT AAAGCAGGAT 
CGGCTGCTTG GCTTGCTGAT GCCCTTTAGG GCTTTTGGGG ATGTAAAGTT CAAATGGAGC 
ATTGACCTTC AAAAGAGAGT GATAGAGTCT GGCCCAGACC AGTTGAATGA CAATGAATAC 
ACCAAGTTTA TCCCTCCTAA CTATCATACA CCTCCTTATC TCACTGCTGA GCCAGAGGTA 
ACTTATCACC GATTAAGGCC ACAGGATAAA TTCCTAGTGT TAGCAACTGA TGGATTGTGG 
GAGACTATGC ATAGACAGGA TGTGGTTAGG ATTGTGGGTG AGTACTTAAC TGGTATGCAT 
CACCAACAGC CAATAGCTGT TGGTGGGTAC AAGGTGACTC TGGGACAGAT GCATGGCCTT 
TTAACAGAAA GGAGAGCAAA GATGTCATCA GTCTTTGAGG ATCAGAATGC AGCAACCCAT 
CTCATTCGCC ATGCTGTAGG CAATAATGAA TTTGGAGCTG TTGATCATGA ACGACTCTCT 
AAAATGCTTA GCCTTCCTGA AGAGCTTGCT CGGATGTATA GAGATGACAT TACAATCATT 
GTAGTTCAGT TCAATTCTCA TGTTGTAGGA GCATATCAAA ACCAGGAACA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pdp1 ( XM_006237908.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006237970.1 Rattus norvegicus pyruvate dehyrogenase phosphatase catalytic subunit 1 (Pdp1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa28056
Clone ID Related Accession (Same CDS sequence)	XM_006237908.2
Accession Version	XM_006237908.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	pyruvate dehydrogenase [acetyl-transferring]-phosphatase 1, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006237908.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGC CGCGGCGCGC CTGTCCGGGG TCCTGGAGTT CCCCGCCTAG GGCTATGATG 
CCGCCTCGCC GGCAGGGAAT CCCGGTCAGA ACATCCAGTC TACCACTGTT CTCTGATGCC 
ATGCCAGCAC CAACTCAACT GTTTTTTCCT CTCGTCCGTA ACTGTGAACT GAGCAGAATC 
TATGGCACTG CATGTTACTG CCACCACAAA CATCTCTGCT GTTCACCACC GTACATTCCT 
CAGAATCGTC TGAGATACAC ACCCCATCCA GCATATGCTA CCTTTTGTAG GCCAAGGGAG 
AACTGGTGGC AGTATACTCA AGGAAGGAGA TATGCTTCTA CGCCTCAGAA ATTTTACCTC 
ACACCTCCAC AAGTCAACAG CATCCTTAAA GCTAATGAAT ACAGCTTCAA AGTACCAGAA 
TTTGATGGCA AAAATGTCAG TTCCATTCTT GGATTTGACA GCAATCAGCT GCCTGCAAAT 
GCACCCATAG AAGACCGGAG AAGTGCAGCA ACCTGCTTGC AGACCAGAGG GATGCTTTTG 
GGCGTTTTTG ATGGTCATGC AGGCTGTGCT TGCTCCCAGG CAGTCAGTGA AAGACTCTTC 
TATTATATTG CTGTTTCCTT GTTACCCCAT GAGACTTTGC TAGAGATTGA AAATGCAGTG 
GAGAGTGGTC GGGCACTGCT ACCTATCCTT CAGTGGCACA AGCACCCCAA TGATTACTTC 
AGTAAGGAGG CATCCAAATT GTATTTCAAC AGCTTGAGGA CTTACTGGCA AGAGCTTATA 
GACCTCAATA CTGGAGAATC AGCTGATATT GATGTTAAGG AGGCTTTAAT TAATGCTTTC 
AAGAGACTTG ATAATGACAT TTCATTGGAG GCTCAAGTCG GTGATCCTAA TTCTTTTCTC 
AATTACCTGG TGCTTCGGGT AGCATTTTCT GGGGCTACTG CTTGTGTGGC CCATGTAGAT 
GGTGTTGACC TCCATGTGGC TAACACTGGC GATAGTAGAG CCATGCTAGG TGTGCAAGAA 
GAAGATGGCT CCTGGTCAGC AGTCACGCTC TCTAATGACC ACAATGCTCA GAATGAAAGA 
GAACTAGAAC GTCTAAAACT GGAACACCCA AAAAATGAGG CCAAGAGCGT GGTAAAGCAG 
GATCGGCTGC TTGGCTTGCT GATGCCCTTT AGGGCTTTTG GGGATGTAAA GTTCAAATGG 
AGCATTGACC TTCAAAAGAG AGTGATAGAG TCTGGCCCAG ACCAGTTGAA TGACAATGAA 
TACACCAAGT TTATCCCTCC TAACTATCAT ACACCTCCTT ATCTCACTGC TGAGCCAGAG 
GTAACTTATC ACCGATTAAG GCCACAGGAT AAATTCCTAG TGTTAGCAAC TGATGGATTG 
TGGGAGACTA TGCATAGACA GGATGTGGTT AGGATTGTGG GTGAGTACTT AACTGGTATG 
CATCACCAAC AGCCAATAGC TGTTGGTGGG TACAAGGTGA CTCTGGGACA GATGCATGGC 
CTTTTAACAG AAAGGAGAGC AAAGATGTCA TCAGTCTTTG AGGATCAGAA TGCAGCAACC 
CATCTCATTC GCCATGCTGT AGGCAATAAT GAATTTGGAG CTGTTGATCA TGAACGACTC 
TCTAAAATGC TTAGCCTTCC TGAAGAGCTT GCTCGGATGT ATAGAGATGA CATTACAATC 
ATTGTAGTTC AGTTCAATTC TCATGTTGTA GGAGCATATC AAAACCAGGA ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006237970.1
CDS	18..1754
Translation	MERPRRACPGSWSSPPRAMMPPRRQGIPVRTSSLPLFSDAMPAPTQLFFPLVRNCELSRIYGTACYCHHKHLCCSPPYIPQNRLRYTPHPAYATFCRPRENWWQYTQGRRYASTPQKFYLTPPQVNSILKANEYSFKVPEFDGKNVSSILGFDSNQLPANAPIEDRRSAATCLQTRGMLLGVFDGHAGCACSQAVSERLFYYIAVSLLPHETLLEIENAVESGRALLPILQWHKHPNDYFSKEASKLYFNSLRTYWQELIDLNTGESADIDVKEALINAFKRLDNDISLEAQVGDPNSFLNYLVLRVAFSGATACVAHVDGVDLHVANTGDSRAMLGVQEEDGSWSAVTLSNDHNAQNERELERLKLEHPKNEAKSVVKQDRLLGLLMPFRAFGDVKFKWSIDLQKRVIESGPDQLNDNEYTKFIPPNYHTPPYLTAEPEVTYHRLRPQDKFLVLATDGLWETMHRQDVVRIVGEYLTGMHHQQPIAVGGYKVTLGQMHGLLTERRAKMSSVFEDQNAATHLIRHAVGNNEFGAVDHERLSKMLSLPEELARMYRDDITIIVVQFNSHVVGAYQNQEQ

Target ORF information:

RefSeq Version	XM_006237908.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pyruvate dehyrogenase phosphatase catalytic subunit 1 (Pdp1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006237908.2

ORF Insert Sequence:

ATGGAGCGGC CGCGGCGCGC CTGTCCGGGG TCCTGGAGTT CCCCGCCTAG GGCTATGATG 
CCGCCTCGCC GGCAGGGAAT CCCGGTCAGA ACATCCAGTC TACCACTGTT CTCTGATGCC 
ATGCCAGCAC CAACTCAACT GTTTTTTCCT CTCGTCCGTA ACTGTGAACT GAGCAGAATC 
TATGGCACTG CATGTTACTG CCACCACAAA CATCTCTGCT GTTCACCACC GTACATTCCT 
CAGAATCGTC TGAGATACAC ACCCCATCCA GCATATGCTA CCTTTTGTAG GCCAAGGGAG 
AACTGGTGGC AGTATACTCA AGGAAGGAGA TATGCTTCTA CGCCTCAGAA ATTTTACCTC 
ACACCTCCAC AAGTCAACAG CATCCTTAAA GCTAATGAAT ACAGCTTCAA AGTACCAGAA 
TTTGATGGCA AAAATGTCAG TTCCATTCTT GGATTTGACA GCAATCAGCT GCCTGCAAAT 
GCACCCATAG AAGACCGGAG AAGTGCAGCA ACCTGCTTGC AGACCAGAGG GATGCTTTTG 
GGCGTTTTTG ATGGTCATGC AGGCTGTGCT TGCTCCCAGG CAGTCAGTGA AAGACTCTTC 
TATTATATTG CTGTTTCCTT GTTACCCCAT GAGACTTTGC TAGAGATTGA AAATGCAGTG 
GAGAGTGGTC GGGCACTGCT ACCTATCCTT CAGTGGCACA AGCACCCCAA TGATTACTTC 
AGTAAGGAGG CATCCAAATT GTATTTCAAC AGCTTGAGGA CTTACTGGCA AGAGCTTATA 
GACCTCAATA CTGGAGAATC AGCTGATATT GATGTTAAGG AGGCTTTAAT TAATGCTTTC 
AAGAGACTTG ATAATGACAT TTCATTGGAG GCTCAAGTCG GTGATCCTAA TTCTTTTCTC 
AATTACCTGG TGCTTCGGGT AGCATTTTCT GGGGCTACTG CTTGTGTGGC CCATGTAGAT 
GGTGTTGACC TCCATGTGGC TAACACTGGC GATAGTAGAG CCATGCTAGG TGTGCAAGAA 
GAAGATGGCT CCTGGTCAGC AGTCACGCTC TCTAATGACC ACAATGCTCA GAATGAAAGA 
GAACTAGAAC GTCTAAAACT GGAACACCCA AAAAATGAGG CCAAGAGCGT GGTAAAGCAG 
GATCGGCTGC TTGGCTTGCT GATGCCCTTT AGGGCTTTTG GGGATGTAAA GTTCAAATGG 
AGCATTGACC TTCAAAAGAG AGTGATAGAG TCTGGCCCAG ACCAGTTGAA TGACAATGAA 
TACACCAAGT TTATCCCTCC TAACTATCAT ACACCTCCTT ATCTCACTGC TGAGCCAGAG 
GTAACTTATC ACCGATTAAG GCCACAGGAT AAATTCCTAG TGTTAGCAAC TGATGGATTG 
TGGGAGACTA TGCATAGACA GGATGTGGTT AGGATTGTGG GTGAGTACTT AACTGGTATG 
CATCACCAAC AGCCAATAGC TGTTGGTGGG TACAAGGTGA CTCTGGGACA GATGCATGGC 
CTTTTAACAG AAAGGAGAGC AAAGATGTCA TCAGTCTTTG AGGATCAGAA TGCAGCAACC 
CATCTCATTC GCCATGCTGT AGGCAATAAT GAATTTGGAG CTGTTGATCA TGAACGACTC 
TCTAAAATGC TTAGCCTTCC TGAAGAGCTT GCTCGGATGT ATAGAGATGA CATTACAATC 
ATTGTAGTTC AGTTCAATTC TCATGTTGTA GGAGCATATC AAAACCAGGA ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pyruvate dehydrogenase phosphatase1 mRNA expression is divergently and dynamically regulated between rat cerebral cortex, hippocampus and thalamus after traumatic brain injury: a potential biomarker of TBI-induced hyper- and hypo-glycaemia and neuronal vulnerability.
Neuroscience letters525(2)140-5(2012 Sep)
Xing G,Ren M,O'Neill JT,Sharma P,Verma A,Watson WD

Ppm1E is an in cellulo AMP-activated protein kinase phosphatase.
Cellular signalling23(1)114-24(2011 Jan)
Voss M,Paterson J,Kelsall IR,Mart?n-Granados C,Hastie CJ,Peggie MW,Cohen PT

Skeletal muscle type comparison of pyruvate dehydrogenase phosphatase activity and isoform expression: effects of obesity and endurance training.
American journal of physiology. Regulatory, integrative and comparative physiology295(4)R1224-30(2008 Oct)
Leblanc PJ,Mulligan M,Antolic A,Macpherson L,Inglis JG,Martin D,Roy BD,Peters SJ

Crystal structure of pyruvate dehydrogenase phosphatase 1 and its functional implications.
Journal of molecular biology370(3)417-26(2007 Jul)
Vassylyev DG,Symersky J

Pyruvate dehydrogenase kinase regulatory mechanisms and inhibition in treating diabetes, heart ischemia, and cancer.
Cellular and molecular life sciences : CMLS64(7-8)830-49(2007 Apr)
Roche TE,Hiromasa Y

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Pdp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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