Slc12a3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc12a3
Entrez Gene ID	54300
Full Name	solute carrier family 12 member 3
Synonyms	NCC,TSC
General protein information	Preferred Names solute carrier family 12 member 3 Names solute carrier family 12 member 3 Na-Cl symporter solute carrier family 12 (sodium/chloride transporter), member 3 solute carrier family 12 (sodium/chloride transporters), member 3 thiazide-sensitive Na-Cl cotransporter thiazide-sensitive sodium-chloride cotransporter
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19p13-p12
Summary	mediates thiazide-sensitive cotransport of sodium and chloride; may play a role in the metabolism of NaCl and other electrolytes [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008772327.1	XP_008770549.1	solute carrier family 12 member 3 isoform X5
XM_008772328.1	XP_008770550.1	solute carrier family 12 member 3 isoform X6
XM_008772325.1	XP_008770547.1	solute carrier family 12 member 3 isoform X2
XM_008772324.1	XP_008770546.1	solute carrier family 12 member 3 isoform X3
XM_008772323.1	XP_008770545.1	solute carrier family 12 member 3 isoform X1
XM_008772326.1	XP_008770548.1	solute carrier family 12 member 3 isoform X4
NM_019345.3	NP_062218.3	solute carrier family 12 member 3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Slc12a3	NP_062288.2
Rattus norvegicus (Norway rat)	Slc12a3	NP_062218.3
Xenopus tropicalis (tropical clawed frog)	slc12a3	XP_002937217.2
Gallus gallus (chicken)	SLC12A3	XP_414059.4
	SLC12A3	XP_001093467.2
Canis lupus familiaris (dog)	SLC12A3	XP_005617594.1
Bos taurus (cattle)	SLC12A3	NP_001193107.1
Danio rerio (zebrafish)	slc12a3	NP_001038545.1
Pan troglodytes (chimpanzee)	SLC12A3	XP_510983.2
Homo sapiens (human)	SLC12A3	NP_000330.2

Gene Ontology
Bibliography

Related articles in PubMed

Rare mutations in SLC12A1 and SLC12A3 protect against hypertension by reducing the activity of renal salt cotransporters.
Acu?a R, Mart?nez-de-la-Maza L, Ponce-Coria J, V?zquez N, Ortal-Vite P, Pacheco-Alvarez D, Bobadilla NA, Gamba G
Journal of hypertension29(3)475-83(2011 Mar)

Expression of the sodium potassium chloride cotransporter (NKCC1) and sodium chloride cotransporter (NCC) and their effects on rat lens transparency.
Chee KN, Vorontsova I, Lim JC, Kistler J, Donaldson PJ
Molecular vision16800-12(2010 May)

Short-term stimulation of the thiazide-sensitive Na+-Cl- cotransporter by vasopressin involves phosphorylation and membrane translocation.
Mutig K, Saritas T, Uchida S, Kahl T, Borowski T, Paliege A, B?hlick A, Bleich M, Shan Q, Bachmann S
American journal of physiology. Renal physiology298(3)F502-9(2010 Mar)

Acute hypertension provokes acute trafficking of distal tubule Na-Cl cotransporter (NCC) to subapical cytoplasmic vesicles.
Lee DH, Riquier AD, Yang LE, Leong PK, Maunsbach AB, McDonough AA
American journal of physiology. Renal physiology296(4)F810-8(2009 Apr)

Altered renal expression of Na(+) transporters and ROMK in protein-deprived rats.
Ruete MC, Carrizo LC, Bocanegra MV, Vall?s PG
Nephron. Physiology111(3)p17-29(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Kidneys respond acutely to hyperkalemic challenges by decreasing the activity of NCC while increasing that of ENaC.
Title: Responses of distal nephron Na

Direct epithelial action of calcineurin inhibitor cyclosporine A is sufficient for the activation of renal NCC.
Title: Calcineurin inhibitor cyclosporine A activates renal Na-K-Cl cotransporters via local and systemic mechanisms.

Data demonstrate that increased levels of NE prevent dietary sodium-evoked suppression of the NCC, via an ANG II-independent mechanism, to stimulate the development of salt-sensitive hypertension.
Title: Norepinephrine-evoked salt-sensitive hypertension requires impaired renal sodium chloride cotransporter activity in Sprague-Dawley rats.

Despite high circulating aldosterone levels in pregnancy, the aldosterone-responsive transporter NCC is not increased in total or activated (phosphorylated) abundance or in apical localization in midpregnancy.
Title: Renal NCC is unchanged in the midpregnant rat and decreased in the late pregnant rat despite avid renal Na+ retention.

Estradiol, progesterone, and prolactin increase renal NaCl cotransporter phosphorylation and activity.
Title: Ovarian hormones and prolactin increase renal NaCl cotransporter phosphorylation.

The following Slc12a3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc12a3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa39877	NM_019345.3 Latest version!	Rattus norvegicus solute carrier family 12 (sodium/chloride transporters), member 3 (Slc12a3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$734.30 $1049.00
ORa43423	XM_008772323.1 Latest version!	Rattus norvegicus solute carrier family 12 member 3 (Slc12a3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa43424	XM_008772324.1 Latest version!	Rattus norvegicus solute carrier family 12 member 3 (Slc12a3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa43425	XM_008772325.1 Latest version!	Rattus norvegicus solute carrier family 12 member 3 (Slc12a3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa43426	XM_008772326.1 Latest version!	Rattus norvegicus solute carrier family 12 member 3 (Slc12a3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa43427	XM_008772327.1 Latest version!	Rattus norvegicus solute carrier family 12 member 3 (Slc12a3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa43428	XM_008772328.1 Latest version!	Rattus norvegicus solute carrier family 12 member 3 (Slc12a3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc12a3 ( NM_019345.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062218.3 Rattus norvegicus solute carrier family 12 (sodium/chloride transporters), member 3 (Slc12a3), mRNA.

Price & Availability↑

CloneID	ORa39877
Clone ID Related Accession (Same CDS sequence)	NM_019345.3
Accession Version	NM_019345.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2012-04-28
Organism	Rattus norvegicus(Norway rat)
Product	solute carrier family 12 member 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U10097.2, AABR03114055.1, AABR03115267.1 and AABR03115555.1. On Oct 12, 2007 this sequence version replaced NM_019345.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: complete on the 3' end.

ATGGCAGAGC TACCCGTGAC AGAGATGCCA GGCGATGCTC TGTGCAGTGG GCGCTTCACC 
ATCAGCACGC TGCTGGGGGG TGACGAGCCC CCACCAGCTG CCTGTGACAA TAGCCAGCCC 
AGCCATTTGA CCCATGGCAG CACCTTGTAC CTACGCACCT TTGGTTACAA CACTATAGAT 
GTGGTACCCG CCTACGAACA CTACGCTAAC AGCGCTCTGC CCGGCGAGCC CCGGAAGGTG 
CGGCCCACCC TGGCTGACCT GCACTCGTTC CTCAAGCAGG AAGGTAGCCA CCTGCATGCC 
CTGGCCTTTG ATGGACGGCC AGGCCATGAG CTGACTGATG GACTTGTGGA AGACGAGACA 
GGCGCCAACA GCGAGAAGAG CCCTGGAGAA CCTGTGCGTT TCGGCTGGGT CAAGGGAGTG 
ATGATCCGAT GCATGCTCAA CATATGGGGT GTGATCCTCT ATCTGCGGCT GCCCTGGATT 
ACGGCGCAGG CGGGCATTGT CCTGACCTGG CTCATCATCC TGCTGTCAGT CATGGTGACA 
TCTATCACCG GCCTCTCTAT CTCGGCCATC TCCACCAATG GCAAGGTCAA GTCGGGTGGT 
ACCTATTTCC TAATTTCTCG GAGTCTGGGA CCTGAGCTGG GGGGCTCCAT TGGCCTCATC 
TTCGCCTTTG CCAATGCCGT GGGTGTGGCC ATGCACACTG TGGGCTTTGC AGAGACCGTA 
CGAGACCTAC TTCAGGAGAA CGGCACACCC ATTGTAGACC CCATCAATGA CATCCGCATC 
ATCGGTGTGG TCACGGTCAC TGTGCTGCTG GCCATCTCGC TGGCTGGGAT GGAGTGGGAG 
TCCAAGGCCC AGGTGCTGTT CTTTCTTGTC ATCATGGTCT CTTTTGCCAA CTACCTGGTG 
GGCACGCTGA TCCCAGCATC TAAGGACAAA GCCTCCAAAG GTTTCTACAG CTACCATGGG 
GACATTTTTG TTCAGAACCT GGTACCCGAC TGGAGAGGCA TCGATGGTAG CTTCTTCGGG 
ATGTTCTCCA TCTTCTTCCC TTCAGCCACA GGCATTCTGG CAGGGGCCAA CATCTCTGGG 
GACCTCAAGG ACCCTGCTGT CGCTATTCCC AAGGGAACAC TCATGGCCAT TTTCTGGACC 
ACCATTTCCT ACCTTGCCAT CTCGGCCACC ATCGGGTCCT GTGTGGTGAG GGATGCCTCT 
GGGGACGTGA ACGACACCGT AACTCCTGGC CCAGGCCTCT GTGAAGGGCT AGCCTGCGGT 
TATGGCTGGA ACTTCACGGA ATGCTCCCAG CAGCACAGCT GCCGCTATGG CCTCATCAAC 
TACTACCAGA CCATGAGCAT GGTGTCTGCC TTCGCACCCC TGATCACAGC CGGCATCTTT 
GGGGCCACCC TGTCCTCGGC CTTGGCCTGT CTTGTCTCAG CTGCCAAGGT CTTCCAGTGC 
CTGTGCGAAG ACCAGCTGTA CCCTCTGATT GGCTTCTTTG GCAAGGGCTA TGGGAAGAAC 
AAGGAACCTG TGCGTGGCTA CTTGCTGGCC TACGCCATCG CGGTGGCCTT CATCATCATT 
GCTGAGCTCA ACACCATCGC TCCCATCATT TCTAACTTCT TCCTCTGCTC CTACGCCCTC 
ATCAACTTCA GCTGCTTCCA TGCCTCCATC ACCAACTCAC CTGGATGGAG ACCCTCCTTC 
CGATACTACA GCAAGTGGGC GGCGCTGTTT GGGGCAGTGA TCTCCGTGGT CATCATGTTC 
CTGCTTACCT GGTGGGCAGC TCTCATTGCC ATTGGAGTCG TTCTCTTCCT CCTGCTCTAT 
GTGATCTACA AGAAGCCAGA GGTAAACTGG GGCTCCTCAG TGCAGGCTGG CTCCTACAAC 
CTGGCCCTGA GCTACTCCGT GGGCCTCAAT GAGGTGGAAG ACCACATCAA GAACTATCGC 
CCCCAGTGCC TGGTGCTTAC AGGACCCCCC AACTTCCGCC CAGCTCTTGT GGACTTTGTG 
AGCACGTTTA CCCAGAACCT AAGCCTGATG ATCTGTGGCC ACGTGCTCAT TGCACCCGGC 
AAACAGAGAG TGCCGGAGCT CCGACTCATC GCCAGCGGAC ACACCAAGTG GCTGAACAAG 
AGGAAGATCA AGGCCTTCTA CTCTGACGTC ATAGCTGAGG ACTTGCGCAG TGGCGTCCAG 
ATTCTCATGC AGGCCTCGGG TCTGGGAAGA ATGAAACCTA ACATTCTGGT GGTTGGCTTC 
AAGAAGAACT GGCAGTCTGC CCACCCAGCC ACACTGGAGG ACTACATTGG CATCCTGCAC 
GATGCCTTTG ACTTCAACTA CGGGGTGTGC ATCATGAGGA TGCGTGAGGG GCTCAATGTC 
TCTGAGGCGC TGCAGACGCA CACAGCCCCA GAGGCACTGG TACAGGAGGA GCAGACCAGC 
ACCATCTTCC AGTCGGAGCA AGGCAAGAAG ACCATTGACA TCTACTGGCT GTTTGACGAT 
GGAGGCCTCA CCCTCCTCAT CCCTTATCTC CTGCACCGCA AGAAGAGATG GGGCAAATGC 
AAGATCCGCG TGTTCGTTGG GGGCCAGATC AACAGGATGG ATGAAGAGAG AAAGGCGATC 
ATTTCTCTGT TGAGCAAGTT CAGACTGGGA TTCCATGAAG TCCACGTCCT TCCTGACATC 
AACCAGAAGC CCCAGGTGGA GCACACCAAG CGGTTTGAGG ACATGATTGC CCCCTTCCGT 
CTCAATGACG GCTTCAAGGA CGAGGCCACT GTTGCCGAGA TGAGGAGGGA CTATCCCTGG 
AAGATCTCAG ACGAGGAGAT TAATAAGAAC AGAATTAAGT CCCTTCGGCA GGTGAGGCTG 
AATGAGATTC TCCTGGATTA CTCCCGGGAT GCTGCTCTGA TCATCCTTAC CTTGCCCATT 
GGAAGGAAGG GGAAGTGCCC CAGTTCACTG TACATGGCCT GGCTGGAGAC CCTGTCCCAG 
GACCTCAGTC CTCCCGTTCT CCTCATCCGA GGTAACCAGG AGAACGTGCT CACCTTTTAC 
TGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062218.3
CDS	13..3021
Translation	MAELPVTEMPGDALCSGRFTISTLLGGDEPPPAACDNSQPSHLTHGSTLYLRTFGYNTIDVVPAYEHYANSALPGEPRKVRPTLADLHSFLKQEGSHLHALAFDGRPGHELTDGLVEDETGANSEKSPGEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWLIILLSVMVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQENGTPIVDPINDIRIIGVVTVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPASKDKASKGFYSYHGDIFVQNLVPDWRGIDGSFFGMFSIFFPSATGILAGANISGDLKDPAVAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGDVNDTVTPGPGLCEGLACGYGWNFTECSQQHSCRYGLINYYQTMSMVSAFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFRYYSKWAALFGAVISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVSTFTQNLSLMICGHVLIAPGKQRVPELRLIASGHTKWLNKRKIKAFYSDVIAEDLRSGVQILMQASGLGRMKPNILVVGFKKNWQSAHPATLEDYIGILHDAFDFNYGVCIMRMREGLNVSEALQTHTAPEALVQEEQTSTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLHRKKRWGKCKIRVFVGGQINRMDEERKAIISLLSKFRLGFHEVHVLPDINQKPQVEHTKRFEDMIAPFRLNDGFKDEATVAEMRRDYPWKISDEEINKNRIKSLRQVRLNEILLDYSRDAALIILTLPIGRKGKCPSSLYMAWLETLSQDLSPPVLLIRGNQENVLTFYCQ

Target ORF information:

RefSeq Version	NM_019345.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 12 (sodium/chloride transporters), member 3 (Slc12a3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019345.3

ORF Insert Sequence:

ATGGCAGAGC TACCCGTGAC AGAGATGCCA GGCGATGCTC TGTGCAGTGG GCGCTTCACC 
ATCAGCACGC TGCTGGGGGG TGACGAGCCC CCACCAGCTG CCTGTGACAA TAGCCAGCCC 
AGCCATTTGA CCCATGGCAG CACCTTGTAC CTACGCACCT TTGGTTACAA CACTATAGAT 
GTGGTACCCG CCTACGAACA CTACGCTAAC AGCGCTCTGC CCGGCGAGCC CCGGAAGGTG 
CGGCCCACCC TGGCTGACCT GCACTCGTTC CTCAAGCAGG AAGGTAGCCA CCTGCATGCC 
CTGGCCTTTG ATGGACGGCC AGGCCATGAG CTGACTGATG GACTTGTGGA AGACGAGACA 
GGCGCCAACA GCGAGAAGAG CCCTGGAGAA CCTGTGCGTT TCGGCTGGGT CAAGGGAGTG 
ATGATCCGAT GCATGCTCAA CATATGGGGT GTGATCCTCT ATCTGCGGCT GCCCTGGATT 
ACGGCGCAGG CGGGCATTGT CCTGACCTGG CTCATCATCC TGCTGTCAGT CATGGTGACA 
TCTATCACCG GCCTCTCTAT CTCGGCCATC TCCACCAATG GCAAGGTCAA GTCGGGTGGT 
ACCTATTTCC TAATTTCTCG GAGTCTGGGA CCTGAGCTGG GGGGCTCCAT TGGCCTCATC 
TTCGCCTTTG CCAATGCCGT GGGTGTGGCC ATGCACACTG TGGGCTTTGC AGAGACCGTA 
CGAGACCTAC TTCAGGAGAA CGGCACACCC ATTGTAGACC CCATCAATGA CATCCGCATC 
ATCGGTGTGG TCACGGTCAC TGTGCTGCTG GCCATCTCGC TGGCTGGGAT GGAGTGGGAG 
TCCAAGGCCC AGGTGCTGTT CTTTCTTGTC ATCATGGTCT CTTTTGCCAA CTACCTGGTG 
GGCACGCTGA TCCCAGCATC TAAGGACAAA GCCTCCAAAG GTTTCTACAG CTACCATGGG 
GACATTTTTG TTCAGAACCT GGTACCCGAC TGGAGAGGCA TCGATGGTAG CTTCTTCGGG 
ATGTTCTCCA TCTTCTTCCC TTCAGCCACA GGCATTCTGG CAGGGGCCAA CATCTCTGGG 
GACCTCAAGG ACCCTGCTGT CGCTATTCCC AAGGGAACAC TCATGGCCAT TTTCTGGACC 
ACCATTTCCT ACCTTGCCAT CTCGGCCACC ATCGGGTCCT GTGTGGTGAG GGATGCCTCT 
GGGGACGTGA ACGACACCGT AACTCCTGGC CCAGGCCTCT GTGAAGGGCT AGCCTGCGGT 
TATGGCTGGA ACTTCACGGA ATGCTCCCAG CAGCACAGCT GCCGCTATGG CCTCATCAAC 
TACTACCAGA CCATGAGCAT GGTGTCTGCC TTCGCACCCC TGATCACAGC CGGCATCTTT 
GGGGCCACCC TGTCCTCGGC CTTGGCCTGT CTTGTCTCAG CTGCCAAGGT CTTCCAGTGC 
CTGTGCGAAG ACCAGCTGTA CCCTCTGATT GGCTTCTTTG GCAAGGGCTA TGGGAAGAAC 
AAGGAACCTG TGCGTGGCTA CTTGCTGGCC TACGCCATCG CGGTGGCCTT CATCATCATT 
GCTGAGCTCA ACACCATCGC TCCCATCATT TCTAACTTCT TCCTCTGCTC CTACGCCCTC 
ATCAACTTCA GCTGCTTCCA TGCCTCCATC ACCAACTCAC CTGGATGGAG ACCCTCCTTC 
CGATACTACA GCAAGTGGGC GGCGCTGTTT GGGGCAGTGA TCTCCGTGGT CATCATGTTC 
CTGCTTACCT GGTGGGCAGC TCTCATTGCC ATTGGAGTCG TTCTCTTCCT CCTGCTCTAT 
GTGATCTACA AGAAGCCAGA GGTAAACTGG GGCTCCTCAG TGCAGGCTGG CTCCTACAAC 
CTGGCCCTGA GCTACTCCGT GGGCCTCAAT GAGGTGGAAG ACCACATCAA GAACTATCGC 
CCCCAGTGCC TGGTGCTTAC AGGACCCCCC AACTTCCGCC CAGCTCTTGT GGACTTTGTG 
AGCACGTTTA CCCAGAACCT AAGCCTGATG ATCTGTGGCC ACGTGCTCAT TGCACCCGGC 
AAACAGAGAG TGCCGGAGCT CCGACTCATC GCCAGCGGAC ACACCAAGTG GCTGAACAAG 
AGGAAGATCA AGGCCTTCTA CTCTGACGTC ATAGCTGAGG ACTTGCGCAG TGGCGTCCAG 
ATTCTCATGC AGGCCTCGGG TCTGGGAAGA ATGAAACCTA ACATTCTGGT GGTTGGCTTC 
AAGAAGAACT GGCAGTCTGC CCACCCAGCC ACACTGGAGG ACTACATTGG CATCCTGCAC 
GATGCCTTTG ACTTCAACTA CGGGGTGTGC ATCATGAGGA TGCGTGAGGG GCTCAATGTC 
TCTGAGGCGC TGCAGACGCA CACAGCCCCA GAGGCACTGG TACAGGAGGA GCAGACCAGC 
ACCATCTTCC AGTCGGAGCA AGGCAAGAAG ACCATTGACA TCTACTGGCT GTTTGACGAT 
GGAGGCCTCA CCCTCCTCAT CCCTTATCTC CTGCACCGCA AGAAGAGATG GGGCAAATGC 
AAGATCCGCG TGTTCGTTGG GGGCCAGATC AACAGGATGG ATGAAGAGAG AAAGGCGATC 
ATTTCTCTGT TGAGCAAGTT CAGACTGGGA TTCCATGAAG TCCACGTCCT TCCTGACATC 
AACCAGAAGC CCCAGGTGGA GCACACCAAG CGGTTTGAGG ACATGATTGC CCCCTTCCGT 
CTCAATGACG GCTTCAAGGA CGAGGCCACT GTTGCCGAGA TGAGGAGGGA CTATCCCTGG 
AAGATCTCAG ACGAGGAGAT TAATAAGAAC AGAATTAAGT CCCTTCGGCA GGTGAGGCTG 
AATGAGATTC TCCTGGATTA CTCCCGGGAT GCTGCTCTGA TCATCCTTAC CTTGCCCATT 
GGAAGGAAGG GGAAGTGCCC CAGTTCACTG TACATGGCCT GGCTGGAGAC CCTGTCCCAG 
GACCTCAGTC CTCCCGTTCT CCTCATCCGA GGTAACCAGG AGAACGTGCT CACCTTTTAC 
TGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a3 ( XM_008772323.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770545.1 Rattus norvegicus solute carrier family 12 member 3 (Slc12a3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43423
Clone ID Related Accession (Same CDS sequence)	XM_008772323.1
Accession Version	XM_008772323.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	solute carrier family 12 member 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC TACCCGTGAC AGAGATGCCA GGCGATGCTC TGTGCAGTGG GCGCTTCACC 
ATCAGCACGC TGCTGGGGGG TGACGAGCCC CCACCAGCTG CCTGTGACAA TAGCCAGCCC 
AGCCATTTGA CCCATGGCAG CACCTTGTAC CTACGCACCT TTGGTTACAA CACTATAGAT 
GTGGTACCCG CCTACGAACA CTACGCTAAC AGCGCTCTGC CCGGCGAGCC CCGGAAGGTG 
CGGCCCACCC TGGCTGACCT GCACTCGTTC CTCAAGCAGG AAGGTAGCCA CCTGCATGCC 
CTGGCCTTTG ATGGACGGCC AGGCCATGAG CTGACTGATG GACTTGTGGA AGACGAGACA 
GGCGCCAACA GCGAGAAGAG CCCTGGAGAA CCTGTGCGTT TCGGCTGGGT CAAGGGAGTG 
ATGATCCGAT GCATGCTCAA CATATGGGGT GTGATCCTCT ATCTGCGGCT GCCCTGGATT 
ACGGCGCAGG CGGGCATTGC ACACCTGGGA TGGCAGCAGC CACCGGTGAG GGTGGAGCAG 
AAGAGAACAT CGGATGCAGA GTCAGCCGTG TCAGGGTACC TCGTCCTGAC CTGGCTCATC 
ATCCTGCTGT CAGTCATGGT GACATCTATC ACCGGCCTCT CTATCTCGGC CATCTCCACC 
AATGGCAAGG TCAAGTCGGG TGGTACCTAT TTCCTAATTT CTCGGAGTCT GGGACCTGAG 
CTGGGGGGCT CCATTGGCCT CATCTTCGCC TTTGCCAATG CCGTGGGTGT GGCCATGCAC 
ACTGTGGGCT TTGCAGAGAC CGTACGAGAC CTACTTCAGG AGAACGGCAC ACCCATTGTA 
GACCCCATCA ATGACATCCG CATCATCGGT GTGGTCACGG TCACTGTGCT GCTGGCCATC 
TCGCTGGCTG GGATGGAGTG GGAGTCCAAG GCCCAGGTGC TGTTCTTTCT TGTCATCATG 
GTCTCTTTTG CCAACTACCT GGTGGGCACG CTGATCCCAG CATCTAAGGA CAAAGCCTCC 
AAAGGTTTCT ACAGCTACCA TGGGGACATT TTTGTTCAGA ACCTGGTACC CGACTGGAGA 
GGCATCGATG GTAGCTTCTT CGGGATGTTC TCCATCTTCT TCCCTTCAGC CACAGGCATT 
CTGGCAGGGG CCAACATCTC TGGGGACCTC AAGGACCCTG CTGTCGCTAT TCCCAAGGGA 
ACACTCATGG CCATTTTCTG GACCACCATT TCCTACCTTG CCATCTCGGC CACCATCGGG 
TCCTGTGTGG TGAGGGATGC CTCTGGGGAC GTGAACGACA CCGTAACTCC TGGCCCAGGC 
CTCTGTGAAG GGCTAGCCTG CGGTTATGGC TGGAACTTCA CGGAATGCTC CCAGCAGCAC 
AGCTGCCGCT ATGGCCTCAT CAACTACTAC CAGACCATGA GCATGGTGTC TGCCTTCGCA 
CCCCTGATCA CAGCCGGCAT CTTTGGGGCC ACCCTGTCCT CGGCCTTGGC CTGTCTTGTC 
TCAGCTGCCA AGGTCTTCCA GTGCCTGTGC GAAGACCAGC TGTACCCTCT GATTGGCTTC 
TTTGGCAAGG GCTATGGGAA GAACAAGGAA CCTGTGCGTG GCTACTTGCT GGCCTACGCC 
ATCGCGGTGG CCTTCATCAT CATTGCTGAG CTCAACACCA TCGCTCCCAT CATTTCTAAC 
TTCTTCCTCT GCTCCTACGC CCTCATCAAC TTCAGCTGCT TCCATGCCTC CATCACCAAC 
TCACCTGGAT GGAGACCCTC CTTCCGATAC TACAGCAAGT GGGCGGCGCT GTTTGGGGCA 
GTGATCTCCG TGGTCATCAT GTTCCTGCTT ACCTGGTGGG CAGCTCTCAT TGCCATTGGA 
GTCGTTCTCT TCCTCCTGCT CTATGTGATC TACAAGAAGC CAGAGGTAAA CTGGGGCTCC 
TCAGTGCAGG CTGGCTCCTA CAACCTGGCC CTGAGCTACT CCGTGGGCCT CAATGAGGTG 
GAAGACCACA TCAAGAACTA TCGCCCCCAG TGCCTGGTGC TTACAGGACC CCCCAACTTC 
CGCCCAGCTC TTGTGGACTT TGTGAGCACG TTTACCCAGA ACCTAAGCCT GATGATCTGT 
GGCCACGTGC TCATTGCACC CGGCAAACAG AGAGTGCCGG AGCTCCGACT CATCGCCAGC 
GGACACACCA AGTGGCTGAA CAAGAGGAAG ATCAAGGCCT TCTACTCTGA CGTCATAGCT 
GAGGACTTGC GCAGTGGCGT CCAGATTCTC ATGCAGGCCT CGGGTCTGGG AAGAATGAAA 
CCTAACATTC TGGTGGTTGG CTTCAAGAAG AACTGGCAGT CTGCCCACCC AGCCACACTG 
GAGGACTACA TTGGCATCCT GCACGATGCC TTTGACTTCA ACTACGGGGT GTGCATCATG 
AGGATGCGTG AGGGGCTCAA TGTCTCTGAG GCGCTGCAGA CGCACACAGC CCCAGAGGCA 
CTGGTACAGG AGGAGCAGAC CAGCACCATC TTCCAGTCGG AGCAAGGCAA GAAGACCATT 
GACATCTACT GGCTGTTTGA CGATGGAGGC CTCACCCTCC TCATCCCTTA TCTCCTGCAC 
CGCAAGAAGA GATGGGGCAA ATGCAAGATC CGCGTGTTCG TTGGGGGCCA GATCAACAGG 
ATGGATGAAG AGAGAAAGGC GATCATTTCT CTGTTGAGCA AGTTCAGACT GGGATTCCAT 
GAAGTCCACG TCCTTCCTGA CATCAACCAG AAGCCCCAGG TGGAGCACAC CAAGCGGTTT 
GAGGACATGA TTGCCCCCTT CCGTCTCAAT GACGGCTTCA AGGACGAGGC CACTGTTGCC 
GAGATGAGGA GGGACTATCC CTGGAAGATC TCAGACGAGG AGATTAATAA GAACAGAATT 
AAGTCCCTTC GGCAGGTGAG GCTGAATGAG ATTCTCCTGG ATTACTCCCG GGATGCTGCT 
CTGATCATCC TTACCTTGCC CATTGGAAGG AAGGGGAAGT GCCCCAGTTC ACTGTACATG 
GCCTGGCTGG AGACCCTGTC CCAGGACCTC AGTCCTCCCG TTCTCCTCAT CCGAGGTAAC 
CAGGAGAACG TGCTCACCTT TTACTGCCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770545.1
CDS	46..3138
Translation	MAELPVTEMPGDALCSGRFTISTLLGGDEPPPAACDNSQPSHLTHGSTLYLRTFGYNTIDVVPAYEHYANSALPGEPRKVRPTLADLHSFLKQEGSHLHALAFDGRPGHELTDGLVEDETGANSEKSPGEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIAHLGWQQPPVRVEQKRTSDAESAVSGYLVLTWLIILLSVMVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQENGTPIVDPINDIRIIGVVTVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPASKDKASKGFYSYHGDIFVQNLVPDWRGIDGSFFGMFSIFFPSATGILAGANISGDLKDPAVAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGDVNDTVTPGPGLCEGLACGYGWNFTECSQQHSCRYGLINYYQTMSMVSAFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFRYYSKWAALFGAVISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVSTFTQNLSLMICGHVLIAPGKQRVPELRLIASGHTKWLNKRKIKAFYSDVIAEDLRSGVQILMQASGLGRMKPNILVVGFKKNWQSAHPATLEDYIGILHDAFDFNYGVCIMRMREGLNVSEALQTHTAPEALVQEEQTSTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLHRKKRWGKCKIRVFVGGQINRMDEERKAIISLLSKFRLGFHEVHVLPDINQKPQVEHTKRFEDMIAPFRLNDGFKDEATVAEMRRDYPWKISDEEINKNRIKSLRQVRLNEILLDYSRDAALIILTLPIGRKGKCPSSLYMAWLETLSQDLSPPVLLIRGNQENVLTFYCQ

Target ORF information:

RefSeq Version	XM_008772323.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 12 member 3 (Slc12a3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772323.1

ORF Insert Sequence:

ATGGCAGAGC TACCCGTGAC AGAGATGCCA GGCGATGCTC TGTGCAGTGG GCGCTTCACC 
ATCAGCACGC TGCTGGGGGG TGACGAGCCC CCACCAGCTG CCTGTGACAA TAGCCAGCCC 
AGCCATTTGA CCCATGGCAG CACCTTGTAC CTACGCACCT TTGGTTACAA CACTATAGAT 
GTGGTACCCG CCTACGAACA CTACGCTAAC AGCGCTCTGC CCGGCGAGCC CCGGAAGGTG 
CGGCCCACCC TGGCTGACCT GCACTCGTTC CTCAAGCAGG AAGGTAGCCA CCTGCATGCC 
CTGGCCTTTG ATGGACGGCC AGGCCATGAG CTGACTGATG GACTTGTGGA AGACGAGACA 
GGCGCCAACA GCGAGAAGAG CCCTGGAGAA CCTGTGCGTT TCGGCTGGGT CAAGGGAGTG 
ATGATCCGAT GCATGCTCAA CATATGGGGT GTGATCCTCT ATCTGCGGCT GCCCTGGATT 
ACGGCGCAGG CGGGCATTGC ACACCTGGGA TGGCAGCAGC CACCGGTGAG GGTGGAGCAG 
AAGAGAACAT CGGATGCAGA GTCAGCCGTG TCAGGGTACC TCGTCCTGAC CTGGCTCATC 
ATCCTGCTGT CAGTCATGGT GACATCTATC ACCGGCCTCT CTATCTCGGC CATCTCCACC 
AATGGCAAGG TCAAGTCGGG TGGTACCTAT TTCCTAATTT CTCGGAGTCT GGGACCTGAG 
CTGGGGGGCT CCATTGGCCT CATCTTCGCC TTTGCCAATG CCGTGGGTGT GGCCATGCAC 
ACTGTGGGCT TTGCAGAGAC CGTACGAGAC CTACTTCAGG AGAACGGCAC ACCCATTGTA 
GACCCCATCA ATGACATCCG CATCATCGGT GTGGTCACGG TCACTGTGCT GCTGGCCATC 
TCGCTGGCTG GGATGGAGTG GGAGTCCAAG GCCCAGGTGC TGTTCTTTCT TGTCATCATG 
GTCTCTTTTG CCAACTACCT GGTGGGCACG CTGATCCCAG CATCTAAGGA CAAAGCCTCC 
AAAGGTTTCT ACAGCTACCA TGGGGACATT TTTGTTCAGA ACCTGGTACC CGACTGGAGA 
GGCATCGATG GTAGCTTCTT CGGGATGTTC TCCATCTTCT TCCCTTCAGC CACAGGCATT 
CTGGCAGGGG CCAACATCTC TGGGGACCTC AAGGACCCTG CTGTCGCTAT TCCCAAGGGA 
ACACTCATGG CCATTTTCTG GACCACCATT TCCTACCTTG CCATCTCGGC CACCATCGGG 
TCCTGTGTGG TGAGGGATGC CTCTGGGGAC GTGAACGACA CCGTAACTCC TGGCCCAGGC 
CTCTGTGAAG GGCTAGCCTG CGGTTATGGC TGGAACTTCA CGGAATGCTC CCAGCAGCAC 
AGCTGCCGCT ATGGCCTCAT CAACTACTAC CAGACCATGA GCATGGTGTC TGCCTTCGCA 
CCCCTGATCA CAGCCGGCAT CTTTGGGGCC ACCCTGTCCT CGGCCTTGGC CTGTCTTGTC 
TCAGCTGCCA AGGTCTTCCA GTGCCTGTGC GAAGACCAGC TGTACCCTCT GATTGGCTTC 
TTTGGCAAGG GCTATGGGAA GAACAAGGAA CCTGTGCGTG GCTACTTGCT GGCCTACGCC 
ATCGCGGTGG CCTTCATCAT CATTGCTGAG CTCAACACCA TCGCTCCCAT CATTTCTAAC 
TTCTTCCTCT GCTCCTACGC CCTCATCAAC TTCAGCTGCT TCCATGCCTC CATCACCAAC 
TCACCTGGAT GGAGACCCTC CTTCCGATAC TACAGCAAGT GGGCGGCGCT GTTTGGGGCA 
GTGATCTCCG TGGTCATCAT GTTCCTGCTT ACCTGGTGGG CAGCTCTCAT TGCCATTGGA 
GTCGTTCTCT TCCTCCTGCT CTATGTGATC TACAAGAAGC CAGAGGTAAA CTGGGGCTCC 
TCAGTGCAGG CTGGCTCCTA CAACCTGGCC CTGAGCTACT CCGTGGGCCT CAATGAGGTG 
GAAGACCACA TCAAGAACTA TCGCCCCCAG TGCCTGGTGC TTACAGGACC CCCCAACTTC 
CGCCCAGCTC TTGTGGACTT TGTGAGCACG TTTACCCAGA ACCTAAGCCT GATGATCTGT 
GGCCACGTGC TCATTGCACC CGGCAAACAG AGAGTGCCGG AGCTCCGACT CATCGCCAGC 
GGACACACCA AGTGGCTGAA CAAGAGGAAG ATCAAGGCCT TCTACTCTGA CGTCATAGCT 
GAGGACTTGC GCAGTGGCGT CCAGATTCTC ATGCAGGCCT CGGGTCTGGG AAGAATGAAA 
CCTAACATTC TGGTGGTTGG CTTCAAGAAG AACTGGCAGT CTGCCCACCC AGCCACACTG 
GAGGACTACA TTGGCATCCT GCACGATGCC TTTGACTTCA ACTACGGGGT GTGCATCATG 
AGGATGCGTG AGGGGCTCAA TGTCTCTGAG GCGCTGCAGA CGCACACAGC CCCAGAGGCA 
CTGGTACAGG AGGAGCAGAC CAGCACCATC TTCCAGTCGG AGCAAGGCAA GAAGACCATT 
GACATCTACT GGCTGTTTGA CGATGGAGGC CTCACCCTCC TCATCCCTTA TCTCCTGCAC 
CGCAAGAAGA GATGGGGCAA ATGCAAGATC CGCGTGTTCG TTGGGGGCCA GATCAACAGG 
ATGGATGAAG AGAGAAAGGC GATCATTTCT CTGTTGAGCA AGTTCAGACT GGGATTCCAT 
GAAGTCCACG TCCTTCCTGA CATCAACCAG AAGCCCCAGG TGGAGCACAC CAAGCGGTTT 
GAGGACATGA TTGCCCCCTT CCGTCTCAAT GACGGCTTCA AGGACGAGGC CACTGTTGCC 
GAGATGAGGA GGGACTATCC CTGGAAGATC TCAGACGAGG AGATTAATAA GAACAGAATT 
AAGTCCCTTC GGCAGGTGAG GCTGAATGAG ATTCTCCTGG ATTACTCCCG GGATGCTGCT 
CTGATCATCC TTACCTTGCC CATTGGAAGG AAGGGGAAGT GCCCCAGTTC ACTGTACATG 
GCCTGGCTGG AGACCCTGTC CCAGGACCTC AGTCCTCCCG TTCTCCTCAT CCGAGGTAAC 
CAGGAGAACG TGCTCACCTT TTACTGCCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a3 ( XM_008772324.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770546.1 Rattus norvegicus solute carrier family 12 member 3 (Slc12a3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa43424
Clone ID Related Accession (Same CDS sequence)	XM_008772324.1
Accession Version	XM_008772324.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	solute carrier family 12 member 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC TACCCGTGAC AGAGATGCCA GGCGATGCTC TGTGCAGTGG GCGCTTCACC 
ATCAGCACGC TGCTGGGGGG TGACGAGCCC CCACCAGCTG CCTGTGACAA TAGCCAGCCC 
AGCCATTTGA CCCATGGCAG CACCTTGTAC CTACGCACCT TTGGTTACAA CACTATAGAT 
GTGGTACCCG CCTACGAACA CTACGCTAAC AGCGCTCTGC CCGGCGAGCC CCGGAAGGTG 
CGGCCCACCC TGGCTGACCT GCACTCGTTC CTCAAGCAGG AAGGTAGCCA CCTGCATGCC 
CTGGCCTTTG ATGGACGGCC AGGCCATGAG CTGACTGATG GACTTGTGGA AGACGAGACA 
GGCGCCAACA GCGAGAAGAG CCCTGGAGAA CCTGTGCGTT TCGGCTGGGT CAAGGGAGTG 
ATGATCCGAT GCATGCTCAA CATATGGGGT GTGATCCTCT ATCTGCGGCT GCCCTGGATT 
ACGGCGCAGG CGGGCATTGC ACACCTGGGA TGGCAGCAGC CACCGGTGAG GGTGGAGCAG 
AAGAGAACAT CGGATGCAGA GTCAGCCGTG TCAGGGTACC TCGTCCTGAC CTGGCTCATC 
ATCCTGCTGT CAGTCATGGT GACATCTATC ACCGGCCTCT CTATCTCGGC CATCTCCACC 
AATGGCAAGG TCAAGTCGGG TGGTACCTAT TTCCTAATTT CTCGGAGTCT GGGACCTGAG 
CTGGGGGGCT CCATTGGCCT CATCTTCGCC TTTGCCAATG CCGTGGGTGT GGCCATGCAC 
ACTGTGGGCT TTGCAGAGAC CGTACGAGAC CTACTTCAGG AGAACGGCAC ACCCATTGTA 
GACCCCATCA ATGACATCCG CATCATCGGT GTGGTCACGG TCACTGTGCT GCTGGCCATC 
TCGCTGGCTG GGATGGAGTG GGAGTCCAAG GCCCAGGTGC TGTTCTTTCT TGTCATCATG 
GTCTCTTTTG CCAACTACCT GGTGGGCACG CTGATCCCAG CATCTAAGGA CAAAGCCTCC 
AAAGGTTTCT ACAGCTACCA TGGGGACATT TTTGTTCAGA ACCTGGTACC CGACTGGAGA 
GGCATCGATG GTAGCTTCTT CGGGATGTTC TCCATCTTCT TCCCTTCAGC CACAGGCATT 
CTGGCAGGGG CCAACATCTC TGGGGACCTC AAGGACCCTG CTGTCGCTAT TCCCAAGGGA 
ACACTCATGG CCATTTTCTG GACCACCATT TCCTACCTTG CCATCTCGGC CACCATCGGG 
TCCTGTGTGG TGAGGGATGC CTCTGGGGAC GTGAACGACA CCGTAACTCC TGGCCCAGGC 
CTCTGTGAAG GGCTAGCCTG CGGTTATGGC TGGAACTTCA CGGAATGCTC CCAGCAGCAC 
AGCTGCCGCT ATGGCCTCAT CAACTACTAC CAGACCATGA GCATGGTGTC TGCCTTCGCA 
CCCCTGATCA CAGCCGGCAT CTTTGGGGCC ACCCTGTCCT CGGCCTTGGC CTGTCTTGTC 
TCAGCTGCCA AGGTCTTCCA GTGCCTGTGC GAAGACCAGC TGTACCCTCT GATTGGCTTC 
TTTGGCAAGG GCTATGGGAA GAACAAGGAA CCTGTGCGTG GCTACTTGCT GGCCTACGCC 
ATCGCGGTGG CCTTCATCAT CATTGCTGAG CTCAACACCA TCGCTCCCAT CATTTCTAAC 
TTCTTCCTCT GCTCCTACGC CCTCATCAAC TTCAGCTGCT TCCATGCCTC CATCACCAAC 
TCACCTGGAT GGAGACCCTC CTTCCGATAC TACAGCAAGT GGGCGGCGCT GTTTGGGGCA 
GTGATCTCCG TGGTCATCAT GTTCCTGCTT ACCTGGTGGG CAGCTCTCAT TGCCATTGGA 
GTCGTTCTCT TCCTCCTGCT CTATGTGATC TACAAGAAGC CAGAGGTAAA CTGGGGCTCC 
TCAGTGCAGG CTGGCTCCTA CAACCTGGCC CTGAGCTACT CCGTGGGCCT CAATGAGGTG 
GAAGACCACA TCAAGAACTA TCGCCCCCAG TGCCTGGTGC TTACAGGACC CCCCAACTTC 
CGCCCAGCTC TTGTGGACTT TGTGAGCACG TTTACCCAGA ACCTAAGCCT GATGATCTGT 
GGCCACGTGC TCATTGCACC CGGCAAACAG AGAGTGCCGG AGCTCCGACT CATCGCCAGC 
GGACACACCA AGTGGCTGAA CAAGAGGAAG ATCAAGGCCT TCTACTCTGA CGTCATAGCT 
GAGGACTTGC GCAGTGGCGT CCAGATTCTC ATGCAGGCCT CGGGTCTGGG AAGAATGAAA 
CCTAACATTC TGGTGGTTGG CTTCAAGAAG AACTGGCAGT CTGCCCACCC AGCCACACTG 
GAGGACTACA TTGGCATCCT GCACGATGCC TTTGACTTCA ACTACGGGGT GTGCATCATG 
AGGATGCGTG AGGGGCTCAA TGTCTCTGAG GCGCTGCAGA CGCACACCCC AGAGGCACTG 
GTACAGGAGG AGCAGACCAG CACCATCTTC CAGTCGGAGC AAGGCAAGAA GACCATTGAC 
ATCTACTGGC TGTTTGACGA TGGAGGCCTC ACCCTCCTCA TCCCTTATCT CCTGCACCGC 
AAGAAGAGAT GGGGCAAATG CAAGATCCGC GTGTTCGTTG GGGGCCAGAT CAACAGGATG 
GATGAAGAGA GAAAGGCGAT CATTTCTCTG TTGAGCAAGT TCAGACTGGG ATTCCATGAA 
GTCCACGTCC TTCCTGACAT CAACCAGAAG CCCCAGGTGG AGCACACCAA GCGGTTTGAG 
GACATGATTG CCCCCTTCCG TCTCAATGAC GGCTTCAAGG ACGAGGCCAC TGTTGCCGAG 
ATGAGGAGGG ACTATCCCTG GAAGATCTCA GACGAGGAGA TTAATAAGAA CAGAATTAAG 
TCCCTTCGGC AGGTGAGGCT GAATGAGATT CTCCTGGATT ACTCCCGGGA TGCTGCTCTG 
ATCATCCTTA CCTTGCCCAT TGGAAGGAAG GGGAAGTGCC CCAGTTCACT GTACATGGCC 
TGGCTGGAGA CCCTGTCCCA GGACCTCAGT CCTCCCGTTC TCCTCATCCG AGGTAACCAG 
GAGAACGTGC TCACCTTTTA CTGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770546.1
CDS	46..3135
Translation	MAELPVTEMPGDALCSGRFTISTLLGGDEPPPAACDNSQPSHLTHGSTLYLRTFGYNTIDVVPAYEHYANSALPGEPRKVRPTLADLHSFLKQEGSHLHALAFDGRPGHELTDGLVEDETGANSEKSPGEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIAHLGWQQPPVRVEQKRTSDAESAVSGYLVLTWLIILLSVMVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQENGTPIVDPINDIRIIGVVTVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPASKDKASKGFYSYHGDIFVQNLVPDWRGIDGSFFGMFSIFFPSATGILAGANISGDLKDPAVAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGDVNDTVTPGPGLCEGLACGYGWNFTECSQQHSCRYGLINYYQTMSMVSAFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFRYYSKWAALFGAVISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVSTFTQNLSLMICGHVLIAPGKQRVPELRLIASGHTKWLNKRKIKAFYSDVIAEDLRSGVQILMQASGLGRMKPNILVVGFKKNWQSAHPATLEDYIGILHDAFDFNYGVCIMRMREGLNVSEALQTHTPEALVQEEQTSTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLHRKKRWGKCKIRVFVGGQINRMDEERKAIISLLSKFRLGFHEVHVLPDINQKPQVEHTKRFEDMIAPFRLNDGFKDEATVAEMRRDYPWKISDEEINKNRIKSLRQVRLNEILLDYSRDAALIILTLPIGRKGKCPSSLYMAWLETLSQDLSPPVLLIRGNQENVLTFYCQ

Target ORF information:

RefSeq Version	XM_008772324.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 12 member 3 (Slc12a3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772324.1

ORF Insert Sequence:

ATGGCAGAGC TACCCGTGAC AGAGATGCCA GGCGATGCTC TGTGCAGTGG GCGCTTCACC 
ATCAGCACGC TGCTGGGGGG TGACGAGCCC CCACCAGCTG CCTGTGACAA TAGCCAGCCC 
AGCCATTTGA CCCATGGCAG CACCTTGTAC CTACGCACCT TTGGTTACAA CACTATAGAT 
GTGGTACCCG CCTACGAACA CTACGCTAAC AGCGCTCTGC CCGGCGAGCC CCGGAAGGTG 
CGGCCCACCC TGGCTGACCT GCACTCGTTC CTCAAGCAGG AAGGTAGCCA CCTGCATGCC 
CTGGCCTTTG ATGGACGGCC AGGCCATGAG CTGACTGATG GACTTGTGGA AGACGAGACA 
GGCGCCAACA GCGAGAAGAG CCCTGGAGAA CCTGTGCGTT TCGGCTGGGT CAAGGGAGTG 
ATGATCCGAT GCATGCTCAA CATATGGGGT GTGATCCTCT ATCTGCGGCT GCCCTGGATT 
ACGGCGCAGG CGGGCATTGC ACACCTGGGA TGGCAGCAGC CACCGGTGAG GGTGGAGCAG 
AAGAGAACAT CGGATGCAGA GTCAGCCGTG TCAGGGTACC TCGTCCTGAC CTGGCTCATC 
ATCCTGCTGT CAGTCATGGT GACATCTATC ACCGGCCTCT CTATCTCGGC CATCTCCACC 
AATGGCAAGG TCAAGTCGGG TGGTACCTAT TTCCTAATTT CTCGGAGTCT GGGACCTGAG 
CTGGGGGGCT CCATTGGCCT CATCTTCGCC TTTGCCAATG CCGTGGGTGT GGCCATGCAC 
ACTGTGGGCT TTGCAGAGAC CGTACGAGAC CTACTTCAGG AGAACGGCAC ACCCATTGTA 
GACCCCATCA ATGACATCCG CATCATCGGT GTGGTCACGG TCACTGTGCT GCTGGCCATC 
TCGCTGGCTG GGATGGAGTG GGAGTCCAAG GCCCAGGTGC TGTTCTTTCT TGTCATCATG 
GTCTCTTTTG CCAACTACCT GGTGGGCACG CTGATCCCAG CATCTAAGGA CAAAGCCTCC 
AAAGGTTTCT ACAGCTACCA TGGGGACATT TTTGTTCAGA ACCTGGTACC CGACTGGAGA 
GGCATCGATG GTAGCTTCTT CGGGATGTTC TCCATCTTCT TCCCTTCAGC CACAGGCATT 
CTGGCAGGGG CCAACATCTC TGGGGACCTC AAGGACCCTG CTGTCGCTAT TCCCAAGGGA 
ACACTCATGG CCATTTTCTG GACCACCATT TCCTACCTTG CCATCTCGGC CACCATCGGG 
TCCTGTGTGG TGAGGGATGC CTCTGGGGAC GTGAACGACA CCGTAACTCC TGGCCCAGGC 
CTCTGTGAAG GGCTAGCCTG CGGTTATGGC TGGAACTTCA CGGAATGCTC CCAGCAGCAC 
AGCTGCCGCT ATGGCCTCAT CAACTACTAC CAGACCATGA GCATGGTGTC TGCCTTCGCA 
CCCCTGATCA CAGCCGGCAT CTTTGGGGCC ACCCTGTCCT CGGCCTTGGC CTGTCTTGTC 
TCAGCTGCCA AGGTCTTCCA GTGCCTGTGC GAAGACCAGC TGTACCCTCT GATTGGCTTC 
TTTGGCAAGG GCTATGGGAA GAACAAGGAA CCTGTGCGTG GCTACTTGCT GGCCTACGCC 
ATCGCGGTGG CCTTCATCAT CATTGCTGAG CTCAACACCA TCGCTCCCAT CATTTCTAAC 
TTCTTCCTCT GCTCCTACGC CCTCATCAAC TTCAGCTGCT TCCATGCCTC CATCACCAAC 
TCACCTGGAT GGAGACCCTC CTTCCGATAC TACAGCAAGT GGGCGGCGCT GTTTGGGGCA 
GTGATCTCCG TGGTCATCAT GTTCCTGCTT ACCTGGTGGG CAGCTCTCAT TGCCATTGGA 
GTCGTTCTCT TCCTCCTGCT CTATGTGATC TACAAGAAGC CAGAGGTAAA CTGGGGCTCC 
TCAGTGCAGG CTGGCTCCTA CAACCTGGCC CTGAGCTACT CCGTGGGCCT CAATGAGGTG 
GAAGACCACA TCAAGAACTA TCGCCCCCAG TGCCTGGTGC TTACAGGACC CCCCAACTTC 
CGCCCAGCTC TTGTGGACTT TGTGAGCACG TTTACCCAGA ACCTAAGCCT GATGATCTGT 
GGCCACGTGC TCATTGCACC CGGCAAACAG AGAGTGCCGG AGCTCCGACT CATCGCCAGC 
GGACACACCA AGTGGCTGAA CAAGAGGAAG ATCAAGGCCT TCTACTCTGA CGTCATAGCT 
GAGGACTTGC GCAGTGGCGT CCAGATTCTC ATGCAGGCCT CGGGTCTGGG AAGAATGAAA 
CCTAACATTC TGGTGGTTGG CTTCAAGAAG AACTGGCAGT CTGCCCACCC AGCCACACTG 
GAGGACTACA TTGGCATCCT GCACGATGCC TTTGACTTCA ACTACGGGGT GTGCATCATG 
AGGATGCGTG AGGGGCTCAA TGTCTCTGAG GCGCTGCAGA CGCACACCCC AGAGGCACTG 
GTACAGGAGG AGCAGACCAG CACCATCTTC CAGTCGGAGC AAGGCAAGAA GACCATTGAC 
ATCTACTGGC TGTTTGACGA TGGAGGCCTC ACCCTCCTCA TCCCTTATCT CCTGCACCGC 
AAGAAGAGAT GGGGCAAATG CAAGATCCGC GTGTTCGTTG GGGGCCAGAT CAACAGGATG 
GATGAAGAGA GAAAGGCGAT CATTTCTCTG TTGAGCAAGT TCAGACTGGG ATTCCATGAA 
GTCCACGTCC TTCCTGACAT CAACCAGAAG CCCCAGGTGG AGCACACCAA GCGGTTTGAG 
GACATGATTG CCCCCTTCCG TCTCAATGAC GGCTTCAAGG ACGAGGCCAC TGTTGCCGAG 
ATGAGGAGGG ACTATCCCTG GAAGATCTCA GACGAGGAGA TTAATAAGAA CAGAATTAAG 
TCCCTTCGGC AGGTGAGGCT GAATGAGATT CTCCTGGATT ACTCCCGGGA TGCTGCTCTG 
ATCATCCTTA CCTTGCCCAT TGGAAGGAAG GGGAAGTGCC CCAGTTCACT GTACATGGCC 
TGGCTGGAGA CCCTGTCCCA GGACCTCAGT CCTCCCGTTC TCCTCATCCG AGGTAACCAG 
GAGAACGTGC TCACCTTTTA CTGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a3 ( XM_008772325.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770547.1 Rattus norvegicus solute carrier family 12 member 3 (Slc12a3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa43425
Clone ID Related Accession (Same CDS sequence)	XM_008772325.1
Accession Version	XM_008772325.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	solute carrier family 12 member 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC TACCCGTGAC AGAGATGCCA GGCGATGCTC TGTGCAGTGG GCGCTTCACC 
ATCAGCACGC TGCTGGGGGG TGACGAGCCC CCACCAGCTG CCTGTGACAA TAGCCAGCCC 
AGCCATTTGA CCCATGGCAG CACCTTGTAC CTACGCACCT TTGGTTACAA CACTATAGAT 
GTGGTACCCG CCTACGAACA CTACGCTAAC AGCGCTCTGC CCGGCGAGCC CCGGAAGGTG 
CGGCCCACCC TGGCTGACCT GCACTCGTTC CTCAAGGAAG GTAGCCACCT GCATGCCCTG 
GCCTTTGATG GACGGCCAGG CCATGAGCTG ACTGATGGAC TTGTGGAAGA CGAGACAGGC 
GCCAACAGCG AGAAGAGCCC TGGAGAACCT GTGCGTTTCG GCTGGGTCAA GGGAGTGATG 
ATCCGATGCA TGCTCAACAT ATGGGGTGTG ATCCTCTATC TGCGGCTGCC CTGGATTACG 
GCGCAGGCGG GCATTGCACA CCTGGGATGG CAGCAGCCAC CGGTGAGGGT GGAGCAGAAG 
AGAACATCGG ATGCAGAGTC AGCCGTGTCA GGGTACCTCG TCCTGACCTG GCTCATCATC 
CTGCTGTCAG TCATGGTGAC ATCTATCACC GGCCTCTCTA TCTCGGCCAT CTCCACCAAT 
GGCAAGGTCA AGTCGGGTGG TACCTATTTC CTAATTTCTC GGAGTCTGGG ACCTGAGCTG 
GGGGGCTCCA TTGGCCTCAT CTTCGCCTTT GCCAATGCCG TGGGTGTGGC CATGCACACT 
GTGGGCTTTG CAGAGACCGT ACGAGACCTA CTTCAGGAGA ACGGCACACC CATTGTAGAC 
CCCATCAATG ACATCCGCAT CATCGGTGTG GTCACGGTCA CTGTGCTGCT GGCCATCTCG 
CTGGCTGGGA TGGAGTGGGA GTCCAAGGCC CAGGTGCTGT TCTTTCTTGT CATCATGGTC 
TCTTTTGCCA ACTACCTGGT GGGCACGCTG ATCCCAGCAT CTAAGGACAA AGCCTCCAAA 
GGTTTCTACA GCTACCATGG GGACATTTTT GTTCAGAACC TGGTACCCGA CTGGAGAGGC 
ATCGATGGTA GCTTCTTCGG GATGTTCTCC ATCTTCTTCC CTTCAGCCAC AGGCATTCTG 
GCAGGGGCCA ACATCTCTGG GGACCTCAAG GACCCTGCTG TCGCTATTCC CAAGGGAACA 
CTCATGGCCA TTTTCTGGAC CACCATTTCC TACCTTGCCA TCTCGGCCAC CATCGGGTCC 
TGTGTGGTGA GGGATGCCTC TGGGGACGTG AACGACACCG TAACTCCTGG CCCAGGCCTC 
TGTGAAGGGC TAGCCTGCGG TTATGGCTGG AACTTCACGG AATGCTCCCA GCAGCACAGC 
TGCCGCTATG GCCTCATCAA CTACTACCAG ACCATGAGCA TGGTGTCTGC CTTCGCACCC 
CTGATCACAG CCGGCATCTT TGGGGCCACC CTGTCCTCGG CCTTGGCCTG TCTTGTCTCA 
GCTGCCAAGG TCTTCCAGTG CCTGTGCGAA GACCAGCTGT ACCCTCTGAT TGGCTTCTTT 
GGCAAGGGCT ATGGGAAGAA CAAGGAACCT GTGCGTGGCT ACTTGCTGGC CTACGCCATC 
GCGGTGGCCT TCATCATCAT TGCTGAGCTC AACACCATCG CTCCCATCAT TTCTAACTTC 
TTCCTCTGCT CCTACGCCCT CATCAACTTC AGCTGCTTCC ATGCCTCCAT CACCAACTCA 
CCTGGATGGA GACCCTCCTT CCGATACTAC AGCAAGTGGG CGGCGCTGTT TGGGGCAGTG 
ATCTCCGTGG TCATCATGTT CCTGCTTACC TGGTGGGCAG CTCTCATTGC CATTGGAGTC 
GTTCTCTTCC TCCTGCTCTA TGTGATCTAC AAGAAGCCAG AGGTAAACTG GGGCTCCTCA 
GTGCAGGCTG GCTCCTACAA CCTGGCCCTG AGCTACTCCG TGGGCCTCAA TGAGGTGGAA 
GACCACATCA AGAACTATCG CCCCCAGTGC CTGGTGCTTA CAGGACCCCC CAACTTCCGC 
CCAGCTCTTG TGGACTTTGT GAGCACGTTT ACCCAGAACC TAAGCCTGAT GATCTGTGGC 
CACGTGCTCA TTGCACCCGG CAAACAGAGA GTGCCGGAGC TCCGACTCAT CGCCAGCGGA 
CACACCAAGT GGCTGAACAA GAGGAAGATC AAGGCCTTCT ACTCTGACGT CATAGCTGAG 
GACTTGCGCA GTGGCGTCCA GATTCTCATG CAGGCCTCGG GTCTGGGAAG AATGAAACCT 
AACATTCTGG TGGTTGGCTT CAAGAAGAAC TGGCAGTCTG CCCACCCAGC CACACTGGAG 
GACTACATTG GCATCCTGCA CGATGCCTTT GACTTCAACT ACGGGGTGTG CATCATGAGG 
ATGCGTGAGG GGCTCAATGT CTCTGAGGCG CTGCAGACGC ACACAGCCCC AGAGGCACTG 
GTACAGGAGG AGCAGACCAG CACCATCTTC CAGTCGGAGC AAGGCAAGAA GACCATTGAC 
ATCTACTGGC TGTTTGACGA TGGAGGCCTC ACCCTCCTCA TCCCTTATCT CCTGCACCGC 
AAGAAGAGAT GGGGCAAATG CAAGATCCGC GTGTTCGTTG GGGGCCAGAT CAACAGGATG 
GATGAAGAGA GAAAGGCGAT CATTTCTCTG TTGAGCAAGT TCAGACTGGG ATTCCATGAA 
GTCCACGTCC TTCCTGACAT CAACCAGAAG CCCCAGGTGG AGCACACCAA GCGGTTTGAG 
GACATGATTG CCCCCTTCCG TCTCAATGAC GGCTTCAAGG ACGAGGCCAC TGTTGCCGAG 
ATGAGGAGGG ACTATCCCTG GAAGATCTCA GACGAGGAGA TTAATAAGAA CAGAATTAAG 
TCCCTTCGGC AGGTGAGGCT GAATGAGATT CTCCTGGATT ACTCCCGGGA TGCTGCTCTG 
ATCATCCTTA CCTTGCCCAT TGGAAGGAAG GGGAAGTGCC CCAGTTCACT GTACATGGCC 
TGGCTGGAGA CCCTGTCCCA GGACCTCAGT CCTCCCGTTC TCCTCATCCG AGGTAACCAG 
GAGAACGTGC TCACCTTTTA CTGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770547.1
CDS	46..3135
Translation	MAELPVTEMPGDALCSGRFTISTLLGGDEPPPAACDNSQPSHLTHGSTLYLRTFGYNTIDVVPAYEHYANSALPGEPRKVRPTLADLHSFLKEGSHLHALAFDGRPGHELTDGLVEDETGANSEKSPGEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIAHLGWQQPPVRVEQKRTSDAESAVSGYLVLTWLIILLSVMVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQENGTPIVDPINDIRIIGVVTVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPASKDKASKGFYSYHGDIFVQNLVPDWRGIDGSFFGMFSIFFPSATGILAGANISGDLKDPAVAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGDVNDTVTPGPGLCEGLACGYGWNFTECSQQHSCRYGLINYYQTMSMVSAFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFRYYSKWAALFGAVISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVSTFTQNLSLMICGHVLIAPGKQRVPELRLIASGHTKWLNKRKIKAFYSDVIAEDLRSGVQILMQASGLGRMKPNILVVGFKKNWQSAHPATLEDYIGILHDAFDFNYGVCIMRMREGLNVSEALQTHTAPEALVQEEQTSTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLHRKKRWGKCKIRVFVGGQINRMDEERKAIISLLSKFRLGFHEVHVLPDINQKPQVEHTKRFEDMIAPFRLNDGFKDEATVAEMRRDYPWKISDEEINKNRIKSLRQVRLNEILLDYSRDAALIILTLPIGRKGKCPSSLYMAWLETLSQDLSPPVLLIRGNQENVLTFYCQ

Target ORF information:

RefSeq Version	XM_008772325.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 12 member 3 (Slc12a3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772325.1

ORF Insert Sequence:

ATGGCAGAGC TACCCGTGAC AGAGATGCCA GGCGATGCTC TGTGCAGTGG GCGCTTCACC 
ATCAGCACGC TGCTGGGGGG TGACGAGCCC CCACCAGCTG CCTGTGACAA TAGCCAGCCC 
AGCCATTTGA CCCATGGCAG CACCTTGTAC CTACGCACCT TTGGTTACAA CACTATAGAT 
GTGGTACCCG CCTACGAACA CTACGCTAAC AGCGCTCTGC CCGGCGAGCC CCGGAAGGTG 
CGGCCCACCC TGGCTGACCT GCACTCGTTC CTCAAGGAAG GTAGCCACCT GCATGCCCTG 
GCCTTTGATG GACGGCCAGG CCATGAGCTG ACTGATGGAC TTGTGGAAGA CGAGACAGGC 
GCCAACAGCG AGAAGAGCCC TGGAGAACCT GTGCGTTTCG GCTGGGTCAA GGGAGTGATG 
ATCCGATGCA TGCTCAACAT ATGGGGTGTG ATCCTCTATC TGCGGCTGCC CTGGATTACG 
GCGCAGGCGG GCATTGCACA CCTGGGATGG CAGCAGCCAC CGGTGAGGGT GGAGCAGAAG 
AGAACATCGG ATGCAGAGTC AGCCGTGTCA GGGTACCTCG TCCTGACCTG GCTCATCATC 
CTGCTGTCAG TCATGGTGAC ATCTATCACC GGCCTCTCTA TCTCGGCCAT CTCCACCAAT 
GGCAAGGTCA AGTCGGGTGG TACCTATTTC CTAATTTCTC GGAGTCTGGG ACCTGAGCTG 
GGGGGCTCCA TTGGCCTCAT CTTCGCCTTT GCCAATGCCG TGGGTGTGGC CATGCACACT 
GTGGGCTTTG CAGAGACCGT ACGAGACCTA CTTCAGGAGA ACGGCACACC CATTGTAGAC 
CCCATCAATG ACATCCGCAT CATCGGTGTG GTCACGGTCA CTGTGCTGCT GGCCATCTCG 
CTGGCTGGGA TGGAGTGGGA GTCCAAGGCC CAGGTGCTGT TCTTTCTTGT CATCATGGTC 
TCTTTTGCCA ACTACCTGGT GGGCACGCTG ATCCCAGCAT CTAAGGACAA AGCCTCCAAA 
GGTTTCTACA GCTACCATGG GGACATTTTT GTTCAGAACC TGGTACCCGA CTGGAGAGGC 
ATCGATGGTA GCTTCTTCGG GATGTTCTCC ATCTTCTTCC CTTCAGCCAC AGGCATTCTG 
GCAGGGGCCA ACATCTCTGG GGACCTCAAG GACCCTGCTG TCGCTATTCC CAAGGGAACA 
CTCATGGCCA TTTTCTGGAC CACCATTTCC TACCTTGCCA TCTCGGCCAC CATCGGGTCC 
TGTGTGGTGA GGGATGCCTC TGGGGACGTG AACGACACCG TAACTCCTGG CCCAGGCCTC 
TGTGAAGGGC TAGCCTGCGG TTATGGCTGG AACTTCACGG AATGCTCCCA GCAGCACAGC 
TGCCGCTATG GCCTCATCAA CTACTACCAG ACCATGAGCA TGGTGTCTGC CTTCGCACCC 
CTGATCACAG CCGGCATCTT TGGGGCCACC CTGTCCTCGG CCTTGGCCTG TCTTGTCTCA 
GCTGCCAAGG TCTTCCAGTG CCTGTGCGAA GACCAGCTGT ACCCTCTGAT TGGCTTCTTT 
GGCAAGGGCT ATGGGAAGAA CAAGGAACCT GTGCGTGGCT ACTTGCTGGC CTACGCCATC 
GCGGTGGCCT TCATCATCAT TGCTGAGCTC AACACCATCG CTCCCATCAT TTCTAACTTC 
TTCCTCTGCT CCTACGCCCT CATCAACTTC AGCTGCTTCC ATGCCTCCAT CACCAACTCA 
CCTGGATGGA GACCCTCCTT CCGATACTAC AGCAAGTGGG CGGCGCTGTT TGGGGCAGTG 
ATCTCCGTGG TCATCATGTT CCTGCTTACC TGGTGGGCAG CTCTCATTGC CATTGGAGTC 
GTTCTCTTCC TCCTGCTCTA TGTGATCTAC AAGAAGCCAG AGGTAAACTG GGGCTCCTCA 
GTGCAGGCTG GCTCCTACAA CCTGGCCCTG AGCTACTCCG TGGGCCTCAA TGAGGTGGAA 
GACCACATCA AGAACTATCG CCCCCAGTGC CTGGTGCTTA CAGGACCCCC CAACTTCCGC 
CCAGCTCTTG TGGACTTTGT GAGCACGTTT ACCCAGAACC TAAGCCTGAT GATCTGTGGC 
CACGTGCTCA TTGCACCCGG CAAACAGAGA GTGCCGGAGC TCCGACTCAT CGCCAGCGGA 
CACACCAAGT GGCTGAACAA GAGGAAGATC AAGGCCTTCT ACTCTGACGT CATAGCTGAG 
GACTTGCGCA GTGGCGTCCA GATTCTCATG CAGGCCTCGG GTCTGGGAAG AATGAAACCT 
AACATTCTGG TGGTTGGCTT CAAGAAGAAC TGGCAGTCTG CCCACCCAGC CACACTGGAG 
GACTACATTG GCATCCTGCA CGATGCCTTT GACTTCAACT ACGGGGTGTG CATCATGAGG 
ATGCGTGAGG GGCTCAATGT CTCTGAGGCG CTGCAGACGC ACACAGCCCC AGAGGCACTG 
GTACAGGAGG AGCAGACCAG CACCATCTTC CAGTCGGAGC AAGGCAAGAA GACCATTGAC 
ATCTACTGGC TGTTTGACGA TGGAGGCCTC ACCCTCCTCA TCCCTTATCT CCTGCACCGC 
AAGAAGAGAT GGGGCAAATG CAAGATCCGC GTGTTCGTTG GGGGCCAGAT CAACAGGATG 
GATGAAGAGA GAAAGGCGAT CATTTCTCTG TTGAGCAAGT TCAGACTGGG ATTCCATGAA 
GTCCACGTCC TTCCTGACAT CAACCAGAAG CCCCAGGTGG AGCACACCAA GCGGTTTGAG 
GACATGATTG CCCCCTTCCG TCTCAATGAC GGCTTCAAGG ACGAGGCCAC TGTTGCCGAG 
ATGAGGAGGG ACTATCCCTG GAAGATCTCA GACGAGGAGA TTAATAAGAA CAGAATTAAG 
TCCCTTCGGC AGGTGAGGCT GAATGAGATT CTCCTGGATT ACTCCCGGGA TGCTGCTCTG 
ATCATCCTTA CCTTGCCCAT TGGAAGGAAG GGGAAGTGCC CCAGTTCACT GTACATGGCC 
TGGCTGGAGA CCCTGTCCCA GGACCTCAGT CCTCCCGTTC TCCTCATCCG AGGTAACCAG 
GAGAACGTGC TCACCTTTTA CTGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a3 ( XM_008772326.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770548.1 Rattus norvegicus solute carrier family 12 member 3 (Slc12a3), transcript variant X4, mRNA.

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CloneID	ORa43426
Clone ID Related Accession (Same CDS sequence)	XM_008772326.1
Accession Version	XM_008772326.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	solute carrier family 12 member 3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC TACCCGTGAC AGAGATGCCA GGCGATGCTC TGTGCAGTGG GCGCTTCACC 
ATCAGCACGC TGCTGGGGGG TGACGAGCCC CCACCAGCTG CCTGTGACAA TAGCCAGCCC 
AGCCATTTGA CCCATGGCAG CACCTTGTAC CTACGCACCT TTGGTTACAA CACTATAGAT 
GTGGTACCCG CCTACGAACA CTACGCTAAC AGCGCTCTGC CCGGCGAGCC CCGGAAGGTG 
CGGCCCACCC TGGCTGACCT GCACTCGTTC CTCAAGGAAG GTAGCCACCT GCATGCCCTG 
GCCTTTGATG GACGGCCAGG CCATGAGCTG ACTGATGGAC TTGTGGAAGA CGAGACAGGC 
GCCAACAGCG AGAAGAGCCC TGGAGAACCT GTGCGTTTCG GCTGGGTCAA GGGAGTGATG 
ATCCGATGCA TGCTCAACAT ATGGGGTGTG ATCCTCTATC TGCGGCTGCC CTGGATTACG 
GCGCAGGCGG GCATTGTCCT GACCTGGCTC ATCATCCTGC TGTCAGTCAT GGTGACATCT 
ATCACCGGCC TCTCTATCTC GGCCATCTCC ACCAATGGCA AGGTCAAGTC GGGTGGTACC 
TATTTCCTAA TTTCTCGGAG TCTGGGACCT GAGCTGGGGG GCTCCATTGG CCTCATCTTC 
GCCTTTGCCA ATGCCGTGGG TGTGGCCATG CACACTGTGG GCTTTGCAGA GACCGTACGA 
GACCTACTTC AGGAGAACGG CACACCCATT GTAGACCCCA TCAATGACAT CCGCATCATC 
GGTGTGGTCA CGGTCACTGT GCTGCTGGCC ATCTCGCTGG CTGGGATGGA GTGGGAGTCC 
AAGGCCCAGG TGCTGTTCTT TCTTGTCATC ATGGTCTCTT TTGCCAACTA CCTGGTGGGC 
ACGCTGATCC CAGCATCTAA GGACAAAGCC TCCAAAGGTT TCTACAGCTA CCATGGGGAC 
ATTTTTGTTC AGAACCTGGT ACCCGACTGG AGAGGCATCG ATGGTAGCTT CTTCGGGATG 
TTCTCCATCT TCTTCCCTTC AGCCACAGGC ATTCTGGCAG GGGCCAACAT CTCTGGGGAC 
CTCAAGGACC CTGCTGTCGC TATTCCCAAG GGAACACTCA TGGCCATTTT CTGGACCACC 
ATTTCCTACC TTGCCATCTC GGCCACCATC GGGTCCTGTG TGGTGAGGGA TGCCTCTGGG 
GACGTGAACG ACACCGTAAC TCCTGGCCCA GGCCTCTGTG AAGGGCTAGC CTGCGGTTAT 
GGCTGGAACT TCACGGAATG CTCCCAGCAG CACAGCTGCC GCTATGGCCT CATCAACTAC 
TACCAGACCA TGAGCATGGT GTCTGCCTTC GCACCCCTGA TCACAGCCGG CATCTTTGGG 
GCCACCCTGT CCTCGGCCTT GGCCTGTCTT GTCTCAGCTG CCAAGGTCTT CCAGTGCCTG 
TGCGAAGACC AGCTGTACCC TCTGATTGGC TTCTTTGGCA AGGGCTATGG GAAGAACAAG 
GAACCTGTGC GTGGCTACTT GCTGGCCTAC GCCATCGCGG TGGCCTTCAT CATCATTGCT 
GAGCTCAACA CCATCGCTCC CATCATTTCT AACTTCTTCC TCTGCTCCTA CGCCCTCATC 
AACTTCAGCT GCTTCCATGC CTCCATCACC AACTCACCTG GATGGAGACC CTCCTTCCGA 
TACTACAGCA AGTGGGCGGC GCTGTTTGGG GCAGTGATCT CCGTGGTCAT CATGTTCCTG 
CTTACCTGGT GGGCAGCTCT CATTGCCATT GGAGTCGTTC TCTTCCTCCT GCTCTATGTG 
ATCTACAAGA AGCCAGAGGT AAACTGGGGC TCCTCAGTGC AGGCTGGCTC CTACAACCTG 
GCCCTGAGCT ACTCCGTGGG CCTCAATGAG GTGGAAGACC ACATCAAGAA CTATCGCCCC 
CAGTGCCTGG TGCTTACAGG ACCCCCCAAC TTCCGCCCAG CTCTTGTGGA CTTTGTGAGC 
ACGTTTACCC AGAACCTAAG CCTGATGATC TGTGGCCACG TGCTCATTGC ACCCGGCAAA 
CAGAGAGTGC CGGAGCTCCG ACTCATCGCC AGCGGACACA CCAAGTGGCT GAACAAGAGG 
AAGATCAAGG CCTTCTACTC TGACGTCATA GCTGAGGACT TGCGCAGTGG CGTCCAGATT 
CTCATGCAGG CCTCGGGTCT GGGAAGAATG AAACCTAACA TTCTGGTGGT TGGCTTCAAG 
AAGAACTGGC AGTCTGCCCA CCCAGCCACA CTGGAGGACT ACATTGGCAT CCTGCACGAT 
GCCTTTGACT TCAACTACGG GGTGTGCATC ATGAGGATGC GTGAGGGGCT CAATGTCTCT 
GAGGCGCTGC AGACGCACAC AGCCCCAGAG GCACTGGTAC AGGAGGAGCA GACCAGCACC 
ATCTTCCAGT CGGAGCAAGG CAAGAAGACC ATTGACATCT ACTGGCTGTT TGACGATGGA 
GGCCTCACCC TCCTCATCCC TTATCTCCTG CACCGCAAGA AGAGATGGGG CAAATGCAAG 
ATCCGCGTGT TCGTTGGGGG CCAGATCAAC AGGATGGATG AAGAGAGAAA GGCGATCATT 
TCTCTGTTGA GCAAGTTCAG ACTGGGATTC CATGAAGTCC ACGTCCTTCC TGACATCAAC 
CAGAAGCCCC AGGTGGAGCA CACCAAGCGG TTTGAGGACA TGATTGCCCC CTTCCGTCTC 
AATGACGGCT TCAAGGACGA GGCCACTGTT GCCGAGATGA GGAGGGACTA TCCCTGGAAG 
ATCTCAGACG AGGAGATTAA TAAGAACAGA ATTAAGTCCC TTCGGCAGGT GAGGCTGAAT 
GAGATTCTCC TGGATTACTC CCGGGATGCT GCTCTGATCA TCCTTACCTT GCCCATTGGA 
AGGAAGGGGA AGTGCCCCAG TTCACTGTAC ATGGCCTGGC TGGAGACCCT GTCCCAGGAC 
CTCAGTCCTC CCGTTCTCCT CATCCGAGGT AACCAGGAGA ACGTGCTCAC CTTTTACTGC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770548.1
CDS	46..3051
Translation	MAELPVTEMPGDALCSGRFTISTLLGGDEPPPAACDNSQPSHLTHGSTLYLRTFGYNTIDVVPAYEHYANSALPGEPRKVRPTLADLHSFLKEGSHLHALAFDGRPGHELTDGLVEDETGANSEKSPGEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWLIILLSVMVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQENGTPIVDPINDIRIIGVVTVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPASKDKASKGFYSYHGDIFVQNLVPDWRGIDGSFFGMFSIFFPSATGILAGANISGDLKDPAVAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGDVNDTVTPGPGLCEGLACGYGWNFTECSQQHSCRYGLINYYQTMSMVSAFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFRYYSKWAALFGAVISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVSTFTQNLSLMICGHVLIAPGKQRVPELRLIASGHTKWLNKRKIKAFYSDVIAEDLRSGVQILMQASGLGRMKPNILVVGFKKNWQSAHPATLEDYIGILHDAFDFNYGVCIMRMREGLNVSEALQTHTAPEALVQEEQTSTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLHRKKRWGKCKIRVFVGGQINRMDEERKAIISLLSKFRLGFHEVHVLPDINQKPQVEHTKRFEDMIAPFRLNDGFKDEATVAEMRRDYPWKISDEEINKNRIKSLRQVRLNEILLDYSRDAALIILTLPIGRKGKCPSSLYMAWLETLSQDLSPPVLLIRGNQENVLTFYCQ

Target ORF information:

RefSeq Version	XM_008772326.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 12 member 3 (Slc12a3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772326.1

ORF Insert Sequence:

ATGGCAGAGC TACCCGTGAC AGAGATGCCA GGCGATGCTC TGTGCAGTGG GCGCTTCACC 
ATCAGCACGC TGCTGGGGGG TGACGAGCCC CCACCAGCTG CCTGTGACAA TAGCCAGCCC 
AGCCATTTGA CCCATGGCAG CACCTTGTAC CTACGCACCT TTGGTTACAA CACTATAGAT 
GTGGTACCCG CCTACGAACA CTACGCTAAC AGCGCTCTGC CCGGCGAGCC CCGGAAGGTG 
CGGCCCACCC TGGCTGACCT GCACTCGTTC CTCAAGGAAG GTAGCCACCT GCATGCCCTG 
GCCTTTGATG GACGGCCAGG CCATGAGCTG ACTGATGGAC TTGTGGAAGA CGAGACAGGC 
GCCAACAGCG AGAAGAGCCC TGGAGAACCT GTGCGTTTCG GCTGGGTCAA GGGAGTGATG 
ATCCGATGCA TGCTCAACAT ATGGGGTGTG ATCCTCTATC TGCGGCTGCC CTGGATTACG 
GCGCAGGCGG GCATTGTCCT GACCTGGCTC ATCATCCTGC TGTCAGTCAT GGTGACATCT 
ATCACCGGCC TCTCTATCTC GGCCATCTCC ACCAATGGCA AGGTCAAGTC GGGTGGTACC 
TATTTCCTAA TTTCTCGGAG TCTGGGACCT GAGCTGGGGG GCTCCATTGG CCTCATCTTC 
GCCTTTGCCA ATGCCGTGGG TGTGGCCATG CACACTGTGG GCTTTGCAGA GACCGTACGA 
GACCTACTTC AGGAGAACGG CACACCCATT GTAGACCCCA TCAATGACAT CCGCATCATC 
GGTGTGGTCA CGGTCACTGT GCTGCTGGCC ATCTCGCTGG CTGGGATGGA GTGGGAGTCC 
AAGGCCCAGG TGCTGTTCTT TCTTGTCATC ATGGTCTCTT TTGCCAACTA CCTGGTGGGC 
ACGCTGATCC CAGCATCTAA GGACAAAGCC TCCAAAGGTT TCTACAGCTA CCATGGGGAC 
ATTTTTGTTC AGAACCTGGT ACCCGACTGG AGAGGCATCG ATGGTAGCTT CTTCGGGATG 
TTCTCCATCT TCTTCCCTTC AGCCACAGGC ATTCTGGCAG GGGCCAACAT CTCTGGGGAC 
CTCAAGGACC CTGCTGTCGC TATTCCCAAG GGAACACTCA TGGCCATTTT CTGGACCACC 
ATTTCCTACC TTGCCATCTC GGCCACCATC GGGTCCTGTG TGGTGAGGGA TGCCTCTGGG 
GACGTGAACG ACACCGTAAC TCCTGGCCCA GGCCTCTGTG AAGGGCTAGC CTGCGGTTAT 
GGCTGGAACT TCACGGAATG CTCCCAGCAG CACAGCTGCC GCTATGGCCT CATCAACTAC 
TACCAGACCA TGAGCATGGT GTCTGCCTTC GCACCCCTGA TCACAGCCGG CATCTTTGGG 
GCCACCCTGT CCTCGGCCTT GGCCTGTCTT GTCTCAGCTG CCAAGGTCTT CCAGTGCCTG 
TGCGAAGACC AGCTGTACCC TCTGATTGGC TTCTTTGGCA AGGGCTATGG GAAGAACAAG 
GAACCTGTGC GTGGCTACTT GCTGGCCTAC GCCATCGCGG TGGCCTTCAT CATCATTGCT 
GAGCTCAACA CCATCGCTCC CATCATTTCT AACTTCTTCC TCTGCTCCTA CGCCCTCATC 
AACTTCAGCT GCTTCCATGC CTCCATCACC AACTCACCTG GATGGAGACC CTCCTTCCGA 
TACTACAGCA AGTGGGCGGC GCTGTTTGGG GCAGTGATCT CCGTGGTCAT CATGTTCCTG 
CTTACCTGGT GGGCAGCTCT CATTGCCATT GGAGTCGTTC TCTTCCTCCT GCTCTATGTG 
ATCTACAAGA AGCCAGAGGT AAACTGGGGC TCCTCAGTGC AGGCTGGCTC CTACAACCTG 
GCCCTGAGCT ACTCCGTGGG CCTCAATGAG GTGGAAGACC ACATCAAGAA CTATCGCCCC 
CAGTGCCTGG TGCTTACAGG ACCCCCCAAC TTCCGCCCAG CTCTTGTGGA CTTTGTGAGC 
ACGTTTACCC AGAACCTAAG CCTGATGATC TGTGGCCACG TGCTCATTGC ACCCGGCAAA 
CAGAGAGTGC CGGAGCTCCG ACTCATCGCC AGCGGACACA CCAAGTGGCT GAACAAGAGG 
AAGATCAAGG CCTTCTACTC TGACGTCATA GCTGAGGACT TGCGCAGTGG CGTCCAGATT 
CTCATGCAGG CCTCGGGTCT GGGAAGAATG AAACCTAACA TTCTGGTGGT TGGCTTCAAG 
AAGAACTGGC AGTCTGCCCA CCCAGCCACA CTGGAGGACT ACATTGGCAT CCTGCACGAT 
GCCTTTGACT TCAACTACGG GGTGTGCATC ATGAGGATGC GTGAGGGGCT CAATGTCTCT 
GAGGCGCTGC AGACGCACAC AGCCCCAGAG GCACTGGTAC AGGAGGAGCA GACCAGCACC 
ATCTTCCAGT CGGAGCAAGG CAAGAAGACC ATTGACATCT ACTGGCTGTT TGACGATGGA 
GGCCTCACCC TCCTCATCCC TTATCTCCTG CACCGCAAGA AGAGATGGGG CAAATGCAAG 
ATCCGCGTGT TCGTTGGGGG CCAGATCAAC AGGATGGATG AAGAGAGAAA GGCGATCATT 
TCTCTGTTGA GCAAGTTCAG ACTGGGATTC CATGAAGTCC ACGTCCTTCC TGACATCAAC 
CAGAAGCCCC AGGTGGAGCA CACCAAGCGG TTTGAGGACA TGATTGCCCC CTTCCGTCTC 
AATGACGGCT TCAAGGACGA GGCCACTGTT GCCGAGATGA GGAGGGACTA TCCCTGGAAG 
ATCTCAGACG AGGAGATTAA TAAGAACAGA ATTAAGTCCC TTCGGCAGGT GAGGCTGAAT 
GAGATTCTCC TGGATTACTC CCGGGATGCT GCTCTGATCA TCCTTACCTT GCCCATTGGA 
AGGAAGGGGA AGTGCCCCAG TTCACTGTAC ATGGCCTGGC TGGAGACCCT GTCCCAGGAC 
CTCAGTCCTC CCGTTCTCCT CATCCGAGGT AACCAGGAGA ACGTGCTCAC CTTTTACTGC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a3 ( XM_008772327.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770549.1 Rattus norvegicus solute carrier family 12 member 3 (Slc12a3), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa43427
Clone ID Related Accession (Same CDS sequence)	XM_008772327.1
Accession Version	XM_008772327.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	solute carrier family 12 member 3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC TACCCGTGAC AGAGATGCCA GGCGATGCTC TGTGCAGTGG GCGCTTCACC 
ATCAGCACGC TGCTGGGGGG TGACGAGCCC CCACCAGCTG CCTGTGACAA TAGCCAGCCC 
AGCCATTTGA CCCATGGCAG CACCTTGTAC CTACGCACCT TTGGTTACAA CACTATAGAT 
GTGGTACCCG CCTACGAACA CTACGCTAAC AGCGCTCTGC CCGGCGAGCC CCGGAAGGTG 
CGGCCCACCC TGGCTGACCT GCACTCGTTC CTCAAGCAGG AAGGTAGCCA CCTGCATGCC 
CTGGCCTTTG ATGGACGGCC AGGCCATGAG CTGACTGATG GACTTGTGGA AGACGAGACA 
GGCGCCAACA GCGAGAAGAG CCCTGGAGAA CCTGTGCGTT TCGGCTGGGT CAAGGGAGTG 
ATGATCCGAT GCATGCTCAA CATATGGGGT GTGATCCTCT ATCTGCGGCT GCCCTGGATT 
ACGGCGCAGG CGGGCATTGT CCTGACCTGG CTCATCATCC TGCTGTCAGT CATGGTGACA 
TCTATCACCG GCCTCTCTAT CTCGGCCATC TCCACCAATG GCAAGGTCAA GTCGGGTGGT 
ACCTATTTCC TAATTTCTCG GAGTCTGGGA CCTGAGCTGG GGGGCTCCAT TGGCCTCATC 
TTCGCCTTTG CCAATGCCGT GGGTGTGGCC ATGCACACTG TGGGCTTTGC AGAGACCGTA 
CGAGACCTAC TTCAGGAGAA CGGCACACCC ATTGTAGACC CCATCAATGA CATCCGCATC 
ATCGGTGTGG TCACGGTCAC TGTGCTGCTG GCCATCTCGC TGGCTGGGAT GGAGTGGGAG 
TCCAAGGCCC AGGTGCTGTT CTTTCTTGTC ATCATGGTCT CTTTTGCCAA CTACCTGGTG 
GGCACGCTGA TCCCAGCATC TAAGGACAAA GCCTCCAAAG GTTTCTACAG CTACCATGGG 
GACATTTTTG TTCAGAACCT GGTACCCGAC TGGAGAGGCA TCGATGGTAG CTTCTTCGGG 
ATGTTCTCCA TCTTCTTCCC TTCAGCCACA GGCATTCTGG CAGGGGCCAA CATCTCTGGG 
GACCTCAAGG ACCCTGCTGT CGCTATTCCC AAGGGAACAC TCATGGCCAT TTTCTGGACC 
ACCATTTCCT ACCTTGCCAT CTCGGCCACC ATCGGGTCCT GTGTGGTGAG GGATGCCTCT 
GGGGACGTGA ACGACACCGT AACTCCTGGC CCAGGCCTCT GTGAAGGGCT AGCCTGCGGT 
TATGGCTGGA ACTTCACGGA ATGCTCCCAG CAGCACAGCT GCCGCTATGG CCTCATCAAC 
TACTACCAGA CCATGAGCAT GGTGTCTGCC TTCGCACCCC TGATCACAGC CGGCATCTTT 
GGGGCCACCC TGTCCTCGGC CTTGGCCTGT CTTGTCTCAG CTGCCAAGGT CTTCCAGTGC 
CTGTGCGAAG ACCAGCTGTA CCCTCTGATT GGCTTCTTTG GCAAGGGCTA TGGGAAGAAC 
AAGGAACCTG TGCGTGGCTA CTTGCTGGCC TACGCCATCG CGGTGGCCTT CATCATCATT 
GCTGAGCTCA ACACCATCGC TCCCATCATT TCTAACTTCT TCCTCTGCTC CTACGCCCTC 
ATCAACTTCA GCTGCTTCCA TGCCTCCATC ACCAACTCAC CTGGATGGAG ACCCTCCTTC 
CGATACTACA GCAAGTGGGC GGCGCTGTTT GGGGCAGTGA TCTCCGTGGT CATCATGTTC 
CTGCTTACCT GGTGGGCAGC TCTCATTGCC ATTGGAGTCG TTCTCTTCCT CCTGCTCTAT 
GTGATCTACA AGAAGCCAGA GGTAAACTGG GGCTCCTCAG TGCAGGCTGG CTCCTACAAC 
CTGGCCCTGA GCTACTCCGT GGGCCTCAAT GAGGTGGAAG ACCACATCAA GAACTATCGC 
CCCCAGTGCC TGGTGCTTAC AGGACCCCCC AACTTCCGCC CAGCTCTTGT GGACTTTGTG 
AGCACGTTTA CCCAGAACCT AAGCCTGATG ATCTGTGGCC ACGTGCTCAT TGCACCCGGC 
AAACAGAGAG TGCCGGAGCT CCGACTCATC GCCAGCGGAC ACACCAAGTG GCTGAACAAG 
AGGAAGATCA AGGCCTTCTA CTCTGACGTC ATAGCTGAGG ACTTGCGCAG TGGCGTCCAG 
ATTCTCATGC AGGCCTCGGG TCTGGGAAGA ATGAAACCTA ACATTCTGGT GGTTGGCTTC 
AAGAAGAACT GGCAGTCTGC CCACCCAGCC ACACTGGAGG ACTACATTGG CATCCTGCAC 
GATGCCTTTG ACTTCAACTA CGGGGTGTGC ATCATGAGGA TGCGTGAGGG GCTCAATGTC 
TCTGAGGCGC TGCAGACGCA CATTAATCCC ATGTTTGACC CCAGAGAGGG AGACAGGAGA 
GCCAGTGGCA GAAAGGCTGG GCCATCTGTG TCTGGCATGG CAGCCCCAGA GGCACTGGTA 
CAGGAGGAGC AGACCAGCAC CATCTTCCAG TCGGAGCAAG GCAAGAAGAC CATTGACATC 
TACTGGCTGT TTGACGATGG AGGCCTCACC CTCCTCATCC CTTATCTCCT GCACCGCAAG 
AAGAGATGGG GCAAATGCAA GATCCGCGTG TTCGTTGGGG GCCAGATCAA CAGGATGGAT 
GAAGAGAGAA AGGCGATCAT TTCTCTGTTG AGCAAGTTCA GACTGGGATT CCATGAAGTC 
CACGTCCTTC CTGACATCAA CCAGAAGCCC CAGGTGGAGC ACACCAAGCG GTTTGAGGAC 
ATGATTGCCC CCTTCCGTCT CAATGACGGC TTCAAGGACG AGGCCACTGT TGCCGAGATG 
AGGAGGGACT ATCCCTGGAA GATCTCAGAC GAGGAGATTA ATAAGAACAG AATTAAGTCC 
CTTCGGCAGG TGAGGCTGAA TGAGATTCTC CTGGATTACT CCCGGGATGC TGCTCTGATC 
ATCCTTACCT TGCCCATTGG AAGGAAGGGG AAGTGCCCCA GTTCACTGTA CATGGCCTGG 
CTGGAGACCC TGTCCCAGGA CCTCAGTCCT CCCGTTCTCC TCATCCGAGG TAACCAGGAG 
AACGTGCTCA CCTTTTACTG CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770549.1
CDS	46..3132
Translation	MAELPVTEMPGDALCSGRFTISTLLGGDEPPPAACDNSQPSHLTHGSTLYLRTFGYNTIDVVPAYEHYANSALPGEPRKVRPTLADLHSFLKQEGSHLHALAFDGRPGHELTDGLVEDETGANSEKSPGEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWLIILLSVMVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQENGTPIVDPINDIRIIGVVTVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPASKDKASKGFYSYHGDIFVQNLVPDWRGIDGSFFGMFSIFFPSATGILAGANISGDLKDPAVAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGDVNDTVTPGPGLCEGLACGYGWNFTECSQQHSCRYGLINYYQTMSMVSAFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFRYYSKWAALFGAVISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVSTFTQNLSLMICGHVLIAPGKQRVPELRLIASGHTKWLNKRKIKAFYSDVIAEDLRSGVQILMQASGLGRMKPNILVVGFKKNWQSAHPATLEDYIGILHDAFDFNYGVCIMRMREGLNVSEALQTHINPMFDPREGDRRASGRKAGPSVSGMAAPEALVQEEQTSTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLHRKKRWGKCKIRVFVGGQINRMDEERKAIISLLSKFRLGFHEVHVLPDINQKPQVEHTKRFEDMIAPFRLNDGFKDEATVAEMRRDYPWKISDEEINKNRIKSLRQVRLNEILLDYSRDAALIILTLPIGRKGKCPSSLYMAWLETLSQDLSPPVLLIRGNQENVLTFYCQ

Target ORF information:

RefSeq Version	XM_008772327.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 12 member 3 (Slc12a3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772327.1

ORF Insert Sequence:

ATGGCAGAGC TACCCGTGAC AGAGATGCCA GGCGATGCTC TGTGCAGTGG GCGCTTCACC 
ATCAGCACGC TGCTGGGGGG TGACGAGCCC CCACCAGCTG CCTGTGACAA TAGCCAGCCC 
AGCCATTTGA CCCATGGCAG CACCTTGTAC CTACGCACCT TTGGTTACAA CACTATAGAT 
GTGGTACCCG CCTACGAACA CTACGCTAAC AGCGCTCTGC CCGGCGAGCC CCGGAAGGTG 
CGGCCCACCC TGGCTGACCT GCACTCGTTC CTCAAGCAGG AAGGTAGCCA CCTGCATGCC 
CTGGCCTTTG ATGGACGGCC AGGCCATGAG CTGACTGATG GACTTGTGGA AGACGAGACA 
GGCGCCAACA GCGAGAAGAG CCCTGGAGAA CCTGTGCGTT TCGGCTGGGT CAAGGGAGTG 
ATGATCCGAT GCATGCTCAA CATATGGGGT GTGATCCTCT ATCTGCGGCT GCCCTGGATT 
ACGGCGCAGG CGGGCATTGT CCTGACCTGG CTCATCATCC TGCTGTCAGT CATGGTGACA 
TCTATCACCG GCCTCTCTAT CTCGGCCATC TCCACCAATG GCAAGGTCAA GTCGGGTGGT 
ACCTATTTCC TAATTTCTCG GAGTCTGGGA CCTGAGCTGG GGGGCTCCAT TGGCCTCATC 
TTCGCCTTTG CCAATGCCGT GGGTGTGGCC ATGCACACTG TGGGCTTTGC AGAGACCGTA 
CGAGACCTAC TTCAGGAGAA CGGCACACCC ATTGTAGACC CCATCAATGA CATCCGCATC 
ATCGGTGTGG TCACGGTCAC TGTGCTGCTG GCCATCTCGC TGGCTGGGAT GGAGTGGGAG 
TCCAAGGCCC AGGTGCTGTT CTTTCTTGTC ATCATGGTCT CTTTTGCCAA CTACCTGGTG 
GGCACGCTGA TCCCAGCATC TAAGGACAAA GCCTCCAAAG GTTTCTACAG CTACCATGGG 
GACATTTTTG TTCAGAACCT GGTACCCGAC TGGAGAGGCA TCGATGGTAG CTTCTTCGGG 
ATGTTCTCCA TCTTCTTCCC TTCAGCCACA GGCATTCTGG CAGGGGCCAA CATCTCTGGG 
GACCTCAAGG ACCCTGCTGT CGCTATTCCC AAGGGAACAC TCATGGCCAT TTTCTGGACC 
ACCATTTCCT ACCTTGCCAT CTCGGCCACC ATCGGGTCCT GTGTGGTGAG GGATGCCTCT 
GGGGACGTGA ACGACACCGT AACTCCTGGC CCAGGCCTCT GTGAAGGGCT AGCCTGCGGT 
TATGGCTGGA ACTTCACGGA ATGCTCCCAG CAGCACAGCT GCCGCTATGG CCTCATCAAC 
TACTACCAGA CCATGAGCAT GGTGTCTGCC TTCGCACCCC TGATCACAGC CGGCATCTTT 
GGGGCCACCC TGTCCTCGGC CTTGGCCTGT CTTGTCTCAG CTGCCAAGGT CTTCCAGTGC 
CTGTGCGAAG ACCAGCTGTA CCCTCTGATT GGCTTCTTTG GCAAGGGCTA TGGGAAGAAC 
AAGGAACCTG TGCGTGGCTA CTTGCTGGCC TACGCCATCG CGGTGGCCTT CATCATCATT 
GCTGAGCTCA ACACCATCGC TCCCATCATT TCTAACTTCT TCCTCTGCTC CTACGCCCTC 
ATCAACTTCA GCTGCTTCCA TGCCTCCATC ACCAACTCAC CTGGATGGAG ACCCTCCTTC 
CGATACTACA GCAAGTGGGC GGCGCTGTTT GGGGCAGTGA TCTCCGTGGT CATCATGTTC 
CTGCTTACCT GGTGGGCAGC TCTCATTGCC ATTGGAGTCG TTCTCTTCCT CCTGCTCTAT 
GTGATCTACA AGAAGCCAGA GGTAAACTGG GGCTCCTCAG TGCAGGCTGG CTCCTACAAC 
CTGGCCCTGA GCTACTCCGT GGGCCTCAAT GAGGTGGAAG ACCACATCAA GAACTATCGC 
CCCCAGTGCC TGGTGCTTAC AGGACCCCCC AACTTCCGCC CAGCTCTTGT GGACTTTGTG 
AGCACGTTTA CCCAGAACCT AAGCCTGATG ATCTGTGGCC ACGTGCTCAT TGCACCCGGC 
AAACAGAGAG TGCCGGAGCT CCGACTCATC GCCAGCGGAC ACACCAAGTG GCTGAACAAG 
AGGAAGATCA AGGCCTTCTA CTCTGACGTC ATAGCTGAGG ACTTGCGCAG TGGCGTCCAG 
ATTCTCATGC AGGCCTCGGG TCTGGGAAGA ATGAAACCTA ACATTCTGGT GGTTGGCTTC 
AAGAAGAACT GGCAGTCTGC CCACCCAGCC ACACTGGAGG ACTACATTGG CATCCTGCAC 
GATGCCTTTG ACTTCAACTA CGGGGTGTGC ATCATGAGGA TGCGTGAGGG GCTCAATGTC 
TCTGAGGCGC TGCAGACGCA CATTAATCCC ATGTTTGACC CCAGAGAGGG AGACAGGAGA 
GCCAGTGGCA GAAAGGCTGG GCCATCTGTG TCTGGCATGG CAGCCCCAGA GGCACTGGTA 
CAGGAGGAGC AGACCAGCAC CATCTTCCAG TCGGAGCAAG GCAAGAAGAC CATTGACATC 
TACTGGCTGT TTGACGATGG AGGCCTCACC CTCCTCATCC CTTATCTCCT GCACCGCAAG 
AAGAGATGGG GCAAATGCAA GATCCGCGTG TTCGTTGGGG GCCAGATCAA CAGGATGGAT 
GAAGAGAGAA AGGCGATCAT TTCTCTGTTG AGCAAGTTCA GACTGGGATT CCATGAAGTC 
CACGTCCTTC CTGACATCAA CCAGAAGCCC CAGGTGGAGC ACACCAAGCG GTTTGAGGAC 
ATGATTGCCC CCTTCCGTCT CAATGACGGC TTCAAGGACG AGGCCACTGT TGCCGAGATG 
AGGAGGGACT ATCCCTGGAA GATCTCAGAC GAGGAGATTA ATAAGAACAG AATTAAGTCC 
CTTCGGCAGG TGAGGCTGAA TGAGATTCTC CTGGATTACT CCCGGGATGC TGCTCTGATC 
ATCCTTACCT TGCCCATTGG AAGGAAGGGG AAGTGCCCCA GTTCACTGTA CATGGCCTGG 
CTGGAGACCC TGTCCCAGGA CCTCAGTCCT CCCGTTCTCC TCATCCGAGG TAACCAGGAG 
AACGTGCTCA CCTTTTACTG CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a3 ( XM_008772328.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770550.1 Rattus norvegicus solute carrier family 12 member 3 (Slc12a3), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa43428
Clone ID Related Accession (Same CDS sequence)	XM_008772328.1
Accession Version	XM_008772328.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	solute carrier family 12 member 3 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC TACCCGTGAC AGAGATGCCA GGCGATGCTC TGTGCAGTGG GCGCTTCACC 
ATCAGCACGC TGCTGGGGGG TGACGAGCCC CCACCAGCTG CCTGTGACAA TAGCCAGCCC 
AGCCATTTGA CCCATGGCAG CACCTTGTAC CTACGCACCT TTGGTTACAA CACTATAGAT 
GTGGTACCCG CCTACGAACA CTACGCTAAC AGCGCTCTGC CCGGCGAGCC CCGGAAGGTG 
CGGCCCACCC TGGCTGACCT GCACTCGTTC CTCAAGGAAG GTAGCCACCT GCATGCCCTG 
GCCTTTGATG GACGGCCAGG CCATGAGCTG ACTGATGGAC TTGTGGAAGA CGAGACAGGC 
GCCAACAGCG AGAAGAGCCC TGGAGAACCT GTGCGTTTCG GCTGGGTCAA GGGAGTGATG 
ATCCGATGCA TGCTCAACAT ATGGGGTGTG ATCCTCTATC TGCGGCTGCC CTGGATTACG 
GCGCAGGCGG GCATTGTCCT GACCTGGCTC ATCATCCTGC TGTCAGTCAT GGTGACATCT 
ATCACCGGCC TCTCTATCTC GGCCATCTCC ACCAATGGCA AGGTCAAGTC GGGTGGTACC 
TATTTCCTAA TTTCTCGGAG TCTGGGACCT GAGCTGGGGG GCTCCATTGG CCTCATCTTC 
GCCTTTGCCA ATGCCGTGGG TGTGGCCATG CACACTGTGG GCTTTGCAGA GACCGTACGA 
GACCTACTTC AGGAGAACGG CACACCCATT GTAGACCCCA TCAATGACAT CCGCATCATC 
GGTGTGGTCA CGGTCACTGT GCTGCTGGCC ATCTCGCTGG CTGGGATGGA GTGGGAGTCC 
AAGGCCCAGG TGCTGTTCTT TCTTGTCATC ATGGTCTCTT TTGCCAACTA CCTGGTGGGC 
ACGCTGATCC CAGCATCTAA GGACAAAGCC TCCAAAGGTT TCTACAGCTA CCATGGGGAC 
ATTTTTGTTC AGAACCTGGT ACCCGACTGG AGAGGCATCG ATGGTAGCTT CTTCGGGATG 
TTCTCCATCT TCTTCCCTTC AGCCACAGGC ATTCTGGCAG GGGCCAACAT CTCTGGGGAC 
CTCAAGGACC CTGCTGTCGC TATTCCCAAG GGAACACTCA TGGCCATTTT CTGGACCACC 
ATTTCCTACC TTGCCATCTC GGCCACCATC GGGTCCTGTG TGGTGAGGGA TGCCTCTGGG 
GACGTGAACG ACACCGTAAC TCCTGGCCCA GGCCTCTGTG AAGGGCTAGC CTGCGGTTAT 
GGCTGGAACT TCACGGAATG CTCCCAGCAG CACAGCTGCC GCTATGGCCT CATCAACTAC 
TACCAGACCA TGAGCATGGT GTCTGCCTTC GCACCCCTGA TCACAGCCGG CATCTTTGGG 
GCCACCCTGT CCTCGGCCTT GGCCTGTCTT GTCTCAGCTG CCAAGGTCTT CCAGTGCCTG 
TGCGAAGACC AGCTGTACCC TCTGATTGGC TTCTTTGGCA AGGGCTATGG GAAGAACAAG 
GAACCTGTGC GTGGCTACTT GCTGGCCTAC GCCATCGCGG TGGCCTTCAT CATCATTGCT 
GAGCTCAACA CCATCGCTCC CATCATTTCT AACTTCTTCC TCTGCTCCTA CGCCCTCATC 
AACTTCAGCT GCTTCCATGC CTCCATCACC AACTCACCTG GATGGAGACC CTCCTTCCGA 
TACTACAGCA AGTGGGCGGC GCTGTTTGGG GCAGTGATCT CCGTGGTCAT CATGTTCCTG 
CTTACCTGGT GGGCAGCTCT CATTGCCATT GGAGTCGTTC TCTTCCTCCT GCTCTATGTG 
ATCTACAAGA AGCCAGAGGT AAACTGGGGC TCCTCAGTGC AGGCTGGCTC CTACAACCTG 
GCCCTGAGCT ACTCCGTGGG CCTCAATGAG GTGGAAGACC ACATCAAGAA CTATCGCCCC 
CAGTGCCTGG TGCTTACAGG ACCCCCCAAC TTCCGCCCAG CTCTTGTGGA CTTTGTGAGC 
ACGTTTACCC AGAACCTAAG CCTGATGATC TGTGGCCACG TGCTCATTGC ACCCGGCAAA 
CAGAGAGTGC CGGAGCTCCG ACTCATCGCC AGCGGACACA CCAAGTGGCT GAACAAGAGG 
AAGATCAAGG CCTTCTACTC TGACGTCATA GCTGAGGACT TGCGCAGTGG CGTCCAGATT 
CTCATGCAGG CCTCGGGTCT GGGAAGAATG AAACCTAACA TTCTGGTGGT TGGCTTCAAG 
AAGAACTGGC AGTCTGCCCA CCCAGCCACA CTGGAGGACT ACATTGGCAT CCTGCACGAT 
GCCTTTGACT TCAACTACGG GGTGTGCATC ATGAGGATGC GTGAGGGGCT CAATGTCTCT 
GAGGCGCTGC AGACGCACAT TAATCCCATG TTTGACCCCA GAGAGGGAGA CAGGAGAGCC 
AGTGGCAGAA AGGCTGGGCC ATCTGTGTCT GGCATGGCAG CCCCAGAGGC ACTGGTACAG 
GAGGAGCAGA CCAGCACCAT CTTCCAGTCG GAGCAAGGCA AGAAGACCAT TGACATCTAC 
TGGCTGTTTG ACGATGGAGG CCTCACCCTC CTCATCCCTT ATCTCCTGCA CCGCAAGAAG 
AGATGGGGCA AATGCAAGAT CCGCGTGTTC GTTGGGGGCC AGATCAACAG GATGGATGAA 
GAGAGAAAGG CGATCATTTC TCTGTTGAGC AAGTTCAGAC TGGGATTCCA TGAAGTCCAC 
GTCCTTCCTG ACATCAACCA GAAGCCCCAG GTGGAGCACA CCAAGCGGTT TGAGGACATG 
ATTGCCCCCT TCCGTCTCAA TGACGGCTTC AAGGACGAGG CCACTGTTGC CGAGATGAGG 
AGGGACTATC CCTGGAAGAT CTCAGACGAG GAGATTAATA AGAACAGAAT TAAGTCCCTT 
CGGCAGGTGA GGCTGAATGA GATTCTCCTG GATTACTCCC GGGATGCTGC TCTGATCATC 
CTTACCTTGC CCATTGGAAG GAAGGGGAAG TGCCCCAGTT CACTGTACAT GGCCTGGCTG 
GAGACCCTGT CCCAGGACCT CAGTCCTCCC GTTCTCCTCA TCCGAGGTAA CCAGGAGAAC 
GTGCTCACCT TTTACTGCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770550.1
CDS	46..3129
Translation	MAELPVTEMPGDALCSGRFTISTLLGGDEPPPAACDNSQPSHLTHGSTLYLRTFGYNTIDVVPAYEHYANSALPGEPRKVRPTLADLHSFLKEGSHLHALAFDGRPGHELTDGLVEDETGANSEKSPGEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWLIILLSVMVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQENGTPIVDPINDIRIIGVVTVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPASKDKASKGFYSYHGDIFVQNLVPDWRGIDGSFFGMFSIFFPSATGILAGANISGDLKDPAVAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGDVNDTVTPGPGLCEGLACGYGWNFTECSQQHSCRYGLINYYQTMSMVSAFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFRYYSKWAALFGAVISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVSTFTQNLSLMICGHVLIAPGKQRVPELRLIASGHTKWLNKRKIKAFYSDVIAEDLRSGVQILMQASGLGRMKPNILVVGFKKNWQSAHPATLEDYIGILHDAFDFNYGVCIMRMREGLNVSEALQTHINPMFDPREGDRRASGRKAGPSVSGMAAPEALVQEEQTSTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLHRKKRWGKCKIRVFVGGQINRMDEERKAIISLLSKFRLGFHEVHVLPDINQKPQVEHTKRFEDMIAPFRLNDGFKDEATVAEMRRDYPWKISDEEINKNRIKSLRQVRLNEILLDYSRDAALIILTLPIGRKGKCPSSLYMAWLETLSQDLSPPVLLIRGNQENVLTFYCQ

Target ORF information:

RefSeq Version	XM_008772328.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 12 member 3 (Slc12a3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772328.1

ORF Insert Sequence:

ATGGCAGAGC TACCCGTGAC AGAGATGCCA GGCGATGCTC TGTGCAGTGG GCGCTTCACC 
ATCAGCACGC TGCTGGGGGG TGACGAGCCC CCACCAGCTG CCTGTGACAA TAGCCAGCCC 
AGCCATTTGA CCCATGGCAG CACCTTGTAC CTACGCACCT TTGGTTACAA CACTATAGAT 
GTGGTACCCG CCTACGAACA CTACGCTAAC AGCGCTCTGC CCGGCGAGCC CCGGAAGGTG 
CGGCCCACCC TGGCTGACCT GCACTCGTTC CTCAAGGAAG GTAGCCACCT GCATGCCCTG 
GCCTTTGATG GACGGCCAGG CCATGAGCTG ACTGATGGAC TTGTGGAAGA CGAGACAGGC 
GCCAACAGCG AGAAGAGCCC TGGAGAACCT GTGCGTTTCG GCTGGGTCAA GGGAGTGATG 
ATCCGATGCA TGCTCAACAT ATGGGGTGTG ATCCTCTATC TGCGGCTGCC CTGGATTACG 
GCGCAGGCGG GCATTGTCCT GACCTGGCTC ATCATCCTGC TGTCAGTCAT GGTGACATCT 
ATCACCGGCC TCTCTATCTC GGCCATCTCC ACCAATGGCA AGGTCAAGTC GGGTGGTACC 
TATTTCCTAA TTTCTCGGAG TCTGGGACCT GAGCTGGGGG GCTCCATTGG CCTCATCTTC 
GCCTTTGCCA ATGCCGTGGG TGTGGCCATG CACACTGTGG GCTTTGCAGA GACCGTACGA 
GACCTACTTC AGGAGAACGG CACACCCATT GTAGACCCCA TCAATGACAT CCGCATCATC 
GGTGTGGTCA CGGTCACTGT GCTGCTGGCC ATCTCGCTGG CTGGGATGGA GTGGGAGTCC 
AAGGCCCAGG TGCTGTTCTT TCTTGTCATC ATGGTCTCTT TTGCCAACTA CCTGGTGGGC 
ACGCTGATCC CAGCATCTAA GGACAAAGCC TCCAAAGGTT TCTACAGCTA CCATGGGGAC 
ATTTTTGTTC AGAACCTGGT ACCCGACTGG AGAGGCATCG ATGGTAGCTT CTTCGGGATG 
TTCTCCATCT TCTTCCCTTC AGCCACAGGC ATTCTGGCAG GGGCCAACAT CTCTGGGGAC 
CTCAAGGACC CTGCTGTCGC TATTCCCAAG GGAACACTCA TGGCCATTTT CTGGACCACC 
ATTTCCTACC TTGCCATCTC GGCCACCATC GGGTCCTGTG TGGTGAGGGA TGCCTCTGGG 
GACGTGAACG ACACCGTAAC TCCTGGCCCA GGCCTCTGTG AAGGGCTAGC CTGCGGTTAT 
GGCTGGAACT TCACGGAATG CTCCCAGCAG CACAGCTGCC GCTATGGCCT CATCAACTAC 
TACCAGACCA TGAGCATGGT GTCTGCCTTC GCACCCCTGA TCACAGCCGG CATCTTTGGG 
GCCACCCTGT CCTCGGCCTT GGCCTGTCTT GTCTCAGCTG CCAAGGTCTT CCAGTGCCTG 
TGCGAAGACC AGCTGTACCC TCTGATTGGC TTCTTTGGCA AGGGCTATGG GAAGAACAAG 
GAACCTGTGC GTGGCTACTT GCTGGCCTAC GCCATCGCGG TGGCCTTCAT CATCATTGCT 
GAGCTCAACA CCATCGCTCC CATCATTTCT AACTTCTTCC TCTGCTCCTA CGCCCTCATC 
AACTTCAGCT GCTTCCATGC CTCCATCACC AACTCACCTG GATGGAGACC CTCCTTCCGA 
TACTACAGCA AGTGGGCGGC GCTGTTTGGG GCAGTGATCT CCGTGGTCAT CATGTTCCTG 
CTTACCTGGT GGGCAGCTCT CATTGCCATT GGAGTCGTTC TCTTCCTCCT GCTCTATGTG 
ATCTACAAGA AGCCAGAGGT AAACTGGGGC TCCTCAGTGC AGGCTGGCTC CTACAACCTG 
GCCCTGAGCT ACTCCGTGGG CCTCAATGAG GTGGAAGACC ACATCAAGAA CTATCGCCCC 
CAGTGCCTGG TGCTTACAGG ACCCCCCAAC TTCCGCCCAG CTCTTGTGGA CTTTGTGAGC 
ACGTTTACCC AGAACCTAAG CCTGATGATC TGTGGCCACG TGCTCATTGC ACCCGGCAAA 
CAGAGAGTGC CGGAGCTCCG ACTCATCGCC AGCGGACACA CCAAGTGGCT GAACAAGAGG 
AAGATCAAGG CCTTCTACTC TGACGTCATA GCTGAGGACT TGCGCAGTGG CGTCCAGATT 
CTCATGCAGG CCTCGGGTCT GGGAAGAATG AAACCTAACA TTCTGGTGGT TGGCTTCAAG 
AAGAACTGGC AGTCTGCCCA CCCAGCCACA CTGGAGGACT ACATTGGCAT CCTGCACGAT 
GCCTTTGACT TCAACTACGG GGTGTGCATC ATGAGGATGC GTGAGGGGCT CAATGTCTCT 
GAGGCGCTGC AGACGCACAT TAATCCCATG TTTGACCCCA GAGAGGGAGA CAGGAGAGCC 
AGTGGCAGAA AGGCTGGGCC ATCTGTGTCT GGCATGGCAG CCCCAGAGGC ACTGGTACAG 
GAGGAGCAGA CCAGCACCAT CTTCCAGTCG GAGCAAGGCA AGAAGACCAT TGACATCTAC 
TGGCTGTTTG ACGATGGAGG CCTCACCCTC CTCATCCCTT ATCTCCTGCA CCGCAAGAAG 
AGATGGGGCA AATGCAAGAT CCGCGTGTTC GTTGGGGGCC AGATCAACAG GATGGATGAA 
GAGAGAAAGG CGATCATTTC TCTGTTGAGC AAGTTCAGAC TGGGATTCCA TGAAGTCCAC 
GTCCTTCCTG ACATCAACCA GAAGCCCCAG GTGGAGCACA CCAAGCGGTT TGAGGACATG 
ATTGCCCCCT TCCGTCTCAA TGACGGCTTC AAGGACGAGG CCACTGTTGC CGAGATGAGG 
AGGGACTATC CCTGGAAGAT CTCAGACGAG GAGATTAATA AGAACAGAAT TAAGTCCCTT 
CGGCAGGTGA GGCTGAATGA GATTCTCCTG GATTACTCCC GGGATGCTGC TCTGATCATC 
CTTACCTTGC CCATTGGAAG GAAGGGGAAG TGCCCCAGTT CACTGTACAT GGCCTGGCTG 
GAGACCCTGT CCCAGGACCT CAGTCCTCCC GTTCTCCTCA TCCGAGGTAA CCAGGAGAAC 
GTGCTCACCT TTTACTGCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Rare mutations in SLC12A1 and SLC12A3 protect against hypertension by reducing the activity of renal salt cotransporters.
Journal of hypertension29(3)475-83(2011 Mar)
Acu?a R,Mart?nez-de-la-Maza L,Ponce-Coria J,V?zquez N,Ortal-Vite P,Pacheco-Alvarez D,Bobadilla NA,Gamba G

Expression of the sodium potassium chloride cotransporter (NKCC1) and sodium chloride cotransporter (NCC) and their effects on rat lens transparency.
Molecular vision16800-12(2010 May)
Chee KN,Vorontsova I,Lim JC,Kistler J,Donaldson PJ

Short-term stimulation of the thiazide-sensitive Na+-Cl- cotransporter by vasopressin involves phosphorylation and membrane translocation.
American journal of physiology. Renal physiology298(3)F502-9(2010 Mar)
Mutig K,Saritas T,Uchida S,Kahl T,Borowski T,Paliege A,B?hlick A,Bleich M,Shan Q,Bachmann S

Acute hypertension provokes acute trafficking of distal tubule Na-Cl cotransporter (NCC) to subapical cytoplasmic vesicles.
American journal of physiology. Renal physiology296(4)F810-8(2009 Apr)
Lee DH,Riquier AD,Yang LE,Leong PK,Maunsbach AB,McDonough AA

Altered renal expression of Na(+) transporters and ROMK in protein-deprived rats.
Nephron. Physiology111(3)p17-29(2009)
Ruete MC,Carrizo LC,Bocanegra MV,Vall?s PG

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Slc12a3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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