Prkg1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prkg1
Entrez Gene ID	54286
Full Name	protein kinase, cGMP-dependent, type 1
Synonyms	Pkgi,cGk1
General protein information	Preferred Names protein kinase, cGMP-dependent, type 1 Names cGMP-dependent protein kinase 1 cGMP dependent protein kinase type 1 protein kinase, cGMP-dependent, type I
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q52
Summary	may play a role in cGMP mediated regulation of renal hemodynamics [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001105731.3	NP_001099201.3	cGMP-dependent protein kinase 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PRKG1	NP_006249.1
Mus musculus (house mouse)	Prkg1	NP_001013855.1
Gallus gallus (chicken)	PRKG1	XP_003641507.2
	PRKG1	XP_001099460.1
Rattus norvegicus (Norway rat)	Prkg1	NP_001099201.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008863	XP_319605.4
Canis lupus familiaris (dog)	PRKG1	XP_851997.2
Bos taurus (cattle)	PRKG1	NP_776861.1
Danio rerio (zebrafish)	prkg1a	NP_957324.1
Drosophila melanogaster (fruit fly)	for	NP_477488.1
Xenopus tropicalis (tropical clawed frog)	LOC100494278	XP_002935474.2
Pan troglodytes (chimpanzee)	PRKG1	XP_001162858.1

Gene Ontology
Bibliography

Related articles in PubMed

Melatonin protects diabetic heart against ischemia-reperfusion injury, role of membrane receptor-dependent cGMP-PKG activation.
Yu LM, Di WC, Dong X, Li Z, Zhang Y, Xue XD, Xu YL, Zhang J, Xiao X, Han JS, Liu Y, Yang Y, Wang HS
Biochimica et biophysica acta1864(2)563-578(2018 Feb)

Tetrandrine prevents monocrotaline-induced pulmonary arterial hypertension in rats through regulation of the protein expression of inducible nitric oxide synthase and cyclic guanosine monophosphate-dependent protein kinase type 1.
Wang X, Yang Y, Yang D, Tong G, Lv S, Lin X, Chen C, Dong W
Journal of vascular surgery64(5)1468-1477(2016 Nov)

Intracellular calcium signaling regulates glomerular filtration barrier permeability: the role of the PKGI?-dependent pathway.
Piwkowska A, Rogacka D, Audzeyenka I, Kasztan M, Angielski S, Jankowski M
FEBS letters590(12)1739-48(2016 06)

Crystal Structure of PKG I:cGMP Complex Reveals a cGMP-Mediated Dimeric Interface that Facilitates cGMP-Induced Activation.
Kim JJ, Lorenz R, Arold ST, Reger AS, Sankaran B, Casteel DE, Herberg FW, Kim C
Structure (London, England : 1993)24(5)710-720(2016 05)

Scutellarin's Cardiovascular Endothelium Protective Mechanism: Important Role of PKG-I?.
Li L, Li L, Chen C, Yang J, Li J, Hu N, Li Y, Zhang D, Guo T, Liu X, Yang W
PloS one10(10)e0139570(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Tetrandrine prevents monocrotaline-induced pulmonary arterial hypertension in rats through regulation of the iNOS and PKG-1 signaling pathway.
Title: Tetrandrine prevents monocrotaline-induced pulmonary arterial hypertension in rats through regulation of the protein expression of inducible nitric oxide synthase and cyclic guanosine monophosphate-dependent protein kinase type 1.

These data suggest the involvement of calcium and PKGIalpha signaling in insulin-enhanced filtration barrier permeability in podocytes.
Title: Intracellular calcium signaling regulates glomerular filtration barrier permeability: the role of the PKGI?-dependent pathway.

The Golgi apparatus regulates PKGIalpha compartmentation and proteolysis.
Title: The Golgi apparatus regulates cGMP-dependent protein kinase I compartmentation and proteolysis.

Our results demonstrate PKG-Ialpha might play an important role in the protective effects of scutellarin on endothelial dysfunction against myocardial ischemic reperfusion injury.
Title: Scutellarin's Cardiovascular Endothelium Protective Mechanism: Important Role of PKG-I?.

Tumor cell implantation into the tibia induced upregulation of cGKI messenger ribonucleic acid and protein in dorsal root ganglia and hyperexcitability in DRG neurons. Continuing activation of this pathway is needed for hyperalgesia and/or allodynia.
Title: cGMP and cGMP-dependent protein kinase I pathway in dorsal root ganglia contributes to bone cancer pain in rats.

The following Prkg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prkg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12288	NM_001105731.3 Latest version!	Rattus norvegicus protein kinase cGMP-dependent 1 (Prkg1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prkg1 ( NM_001105731.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099201.3 Rattus norvegicus protein kinase cGMP-dependent 1 (Prkg1), mRNA.

Price & Availability↑

CloneID	ORa12288
Clone ID Related Accession (Same CDS sequence)	NM_001105731.3
Accession Version	NM_001105731.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Rattus norvegicus(Norway rat)
Product	cGMP-dependent protein kinase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAHX01000119.1, AAHX01000118.1, AAHX01000105.1, AAHX01000086.1, AAHX01000084.1, AAHX01000077.1, AAHX01000073.1, AAHX01000072.1 and AAHX01000070.1. On Nov 27, 2012 this sequence version replaced NM_001105731.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the Celera genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU251189.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2689601 [ECO:0000350] ##Evidence-Data-END##

ATGAGCGAAC TGGAGGAAGA CTTTGCGAAG ATTCTCATGC TCAAGGAGGA GAGGATCAAA 
GAGCTGGAGA AGCGGCTGTC AGAGAAAGAG GAAGAAATCC AGGAGCTGAA GAGGAAACTC 
CACAAATGCC AGTCGGTGCT GCCCGTGCCC TCCACCCACA TTGGCCCGCG GACCACCCGG 
GCGCAGGGCA TCTCCGCGGA GCCGCAGACC TACAGGTCCT TCCACGACCT GCGCCAGGCG 
TTCCGGAAGT TCACTAAATC CGAGAGGTCG AAGGATCTCA TAAAGGAGGC TATCCTTGAC 
AATGACTTCA TGAAGAACTT GGAGCTGTCA CAGATCCAGG AGATTGTGGA TTGTATGTAC 
CCCGTGGAAT ATGGGAAGGA CAGCTGCATC ATCAAGGAAG GCGATGTGGG GTCACTGGTG 
TATGTCATGG AAGATGGGAA GGTCGAAGTC ACAAAAGAAG GTGTGAAGTT GTGCACCATG 
GGTCCCGGGA AAGTGTTCGG GGAGCTGGCT ATTCTTTACA ACTGTACCCG GACAGCGACC 
GTCAAGACTC TTGTAAATGT GAAACTCTGG GCCATTGATC GACAATGTTT TCAAACAATA 
ATGATGAGAA CAGGACTCAT CAAGCACACC GAGTACATGG AGTTTTTAAA AAGTGTTCCA 
ACATTCCAGA GCCTTCCTGA TGAAATCCTC AGCAAGCTGG CTGATGTCCT CGAAGAGACT 
CACTATGAAA ATGGAGAATA TATCATAAGG CAGGGTGCAA GAGGAGACAC CTTCTTCATC 
ATCAGCAAAG GGAAGGTAAA CGTTACCCGA GAAGACTCAC CAAGTGAAGA CCCAGTCTTT 
CTTAGAACTT TAGGGAAGGG AGATTGGTTT GGAGAGAAAG CCTTGCAGGG GGAGGATGTA 
AGAACAGCGA ACGTCATTGC TGCAGAGGCT GTCACCTGCC TCGTGATTGA CAGAGACTCT 
TTCAAGCATT TAATTGGAGG ACTGGATGAT GTCTCTAACA AAGCATATGA GGATGCAGAA 
GCGAAAGCAA AATATGAAGC CGAAGCTGCC TTCTTCGCCA ACCTGAAGCT GTCTGACTTC 
AACATCATTG ACACCCTTGG AGTGGGAGGT TTCGGACGAG TGGAGCTGGT CCAGTTGAAG 
AGTGAAGAAT CTAAAACATT TGCGATGAAG ATCCTCAAGA AACGACACAT TGTGGACACG 
AGACAGCAGG AGCACATCCG CTCAGAGAAG CAGATCATGC AGGGGGCTCA TTCCGATTTC 
ATCGTGAGGC TATACCGGAC ATTTAAAGAC AGCAAATACT TGTATATGTT GATGGAAGCT 
TGCCTAGGTG GAGAACTCTG GACTATTCTC AGGGATAGGG GTTCATTTGA AGACTCGACA 
ACCAGATTTT ACACAGCATG TGTGGTAGAA GCGTTTGCCT ATCTGCATTC CAAAGGAATC 
ATTTACAGGG ACCTCAAGCC AGAAAATCTC ATCCTAGATC ACCGAGGTTA TGCCAAACTG 
GTTGACTTTG GCTTTGCAAA GAAAATAGGA TTTGGAAAGA AAACATGGAC TTTTTGTGGG 
ACCCCAGAAT ACGTGGCCCC AGAGATCATC CTGAACAAAG GCCATGACAT TTCTGCTGAC 
TACTGGTCAC TAGGGATTCT GATGTATGAG CTTCTGACTG GCAGCCCACC TTTCTCAGGC 
CCAGATCCTA TGAAAACCTA CAACATCATA CTGCGGGGGA TTGACATGAT AGAATTTCCA 
AAGAAGATTG CAAAAAATGC TGCTAATTTA ATTAAAAAAC TATGCAGGGA CAACCCATCA 
GAAAGGTTAG GAAATTTGAA AAACGGAGTC AAAGACATTC AAAAACACAA GTGGTTTGAG 
GGCTTTAACT GGGAAGGCTT AAGAAAAGGC ACCTTGACTC CTCCCATAAT TCCGAGCGTT 
GCGTCGCCCA CGGACACAAG CAATTTTGAC AGTTTCCCTG AGGACAGCGA CGAGCCACCG 
CCTGATGACA ACTCAGGCTG GGACATAGAC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099201.3
CDS	1..2016
Translation	MSELEEDFAKILMLKEERIKELEKRLSEKEEEIQELKRKLHKCQSVLPVPSTHIGPRTTRAQGISAEPQTYRSFHDLRQAFRKFTKSERSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYGKDSCIIKEGDVGSLVYVMEDGKVEVTKEGVKLCTMGPGKVFGELAILYNCTRTATVKTLVNVKLWAIDRQCFQTIMMRTGLIKHTEYMEFLKSVPTFQSLPDEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGKVNVTREDSPSEDPVFLRTLGKGDWFGEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKAYEDAEAKAKYEAEAAFFANLKLSDFNIIDTLGVGGFGRVELVQLKSEESKTFAMKILKKRHIVDTRQQEHIRSEKQIMQGAHSDFIVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYTACVVEAFAYLHSKGIIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISADYWSLGILMYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKIAKNAANLIKKLCRDNPSERLGNLKNGVKDIQKHKWFEGFNWEGLRKGTLTPPIIPSVASPTDTSNFDSFPEDSDEPPPDDNSGWDIDF

Target ORF information:

RefSeq Version	NM_001105731.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase cGMP-dependent 1 (Prkg1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105731.3

ORF Insert Sequence:

ATGAGCGAAC TGGAGGAAGA CTTTGCGAAG ATTCTCATGC TCAAGGAGGA GAGGATCAAA 
GAGCTGGAGA AGCGGCTGTC AGAGAAAGAG GAAGAAATCC AGGAGCTGAA GAGGAAACTC 
CACAAATGCC AGTCGGTGCT GCCCGTGCCC TCCACCCACA TTGGCCCGCG GACCACCCGG 
GCGCAGGGCA TCTCCGCGGA GCCGCAGACC TACAGGTCCT TCCACGACCT GCGCCAGGCG 
TTCCGGAAGT TCACTAAATC CGAGAGGTCG AAGGATCTCA TAAAGGAGGC TATCCTTGAC 
AATGACTTCA TGAAGAACTT GGAGCTGTCA CAGATCCAGG AGATTGTGGA TTGTATGTAC 
CCCGTGGAAT ATGGGAAGGA CAGCTGCATC ATCAAGGAAG GCGATGTGGG GTCACTGGTG 
TATGTCATGG AAGATGGGAA GGTCGAAGTC ACAAAAGAAG GTGTGAAGTT GTGCACCATG 
GGTCCCGGGA AAGTGTTCGG GGAGCTGGCT ATTCTTTACA ACTGTACCCG GACAGCGACC 
GTCAAGACTC TTGTAAATGT GAAACTCTGG GCCATTGATC GACAATGTTT TCAAACAATA 
ATGATGAGAA CAGGACTCAT CAAGCACACC GAGTACATGG AGTTTTTAAA AAGTGTTCCA 
ACATTCCAGA GCCTTCCTGA TGAAATCCTC AGCAAGCTGG CTGATGTCCT CGAAGAGACT 
CACTATGAAA ATGGAGAATA TATCATAAGG CAGGGTGCAA GAGGAGACAC CTTCTTCATC 
ATCAGCAAAG GGAAGGTAAA CGTTACCCGA GAAGACTCAC CAAGTGAAGA CCCAGTCTTT 
CTTAGAACTT TAGGGAAGGG AGATTGGTTT GGAGAGAAAG CCTTGCAGGG GGAGGATGTA 
AGAACAGCGA ACGTCATTGC TGCAGAGGCT GTCACCTGCC TCGTGATTGA CAGAGACTCT 
TTCAAGCATT TAATTGGAGG ACTGGATGAT GTCTCTAACA AAGCATATGA GGATGCAGAA 
GCGAAAGCAA AATATGAAGC CGAAGCTGCC TTCTTCGCCA ACCTGAAGCT GTCTGACTTC 
AACATCATTG ACACCCTTGG AGTGGGAGGT TTCGGACGAG TGGAGCTGGT CCAGTTGAAG 
AGTGAAGAAT CTAAAACATT TGCGATGAAG ATCCTCAAGA AACGACACAT TGTGGACACG 
AGACAGCAGG AGCACATCCG CTCAGAGAAG CAGATCATGC AGGGGGCTCA TTCCGATTTC 
ATCGTGAGGC TATACCGGAC ATTTAAAGAC AGCAAATACT TGTATATGTT GATGGAAGCT 
TGCCTAGGTG GAGAACTCTG GACTATTCTC AGGGATAGGG GTTCATTTGA AGACTCGACA 
ACCAGATTTT ACACAGCATG TGTGGTAGAA GCGTTTGCCT ATCTGCATTC CAAAGGAATC 
ATTTACAGGG ACCTCAAGCC AGAAAATCTC ATCCTAGATC ACCGAGGTTA TGCCAAACTG 
GTTGACTTTG GCTTTGCAAA GAAAATAGGA TTTGGAAAGA AAACATGGAC TTTTTGTGGG 
ACCCCAGAAT ACGTGGCCCC AGAGATCATC CTGAACAAAG GCCATGACAT TTCTGCTGAC 
TACTGGTCAC TAGGGATTCT GATGTATGAG CTTCTGACTG GCAGCCCACC TTTCTCAGGC 
CCAGATCCTA TGAAAACCTA CAACATCATA CTGCGGGGGA TTGACATGAT AGAATTTCCA 
AAGAAGATTG CAAAAAATGC TGCTAATTTA ATTAAAAAAC TATGCAGGGA CAACCCATCA 
GAAAGGTTAG GAAATTTGAA AAACGGAGTC AAAGACATTC AAAAACACAA GTGGTTTGAG 
GGCTTTAACT GGGAAGGCTT AAGAAAAGGC ACCTTGACTC CTCCCATAAT TCCGAGCGTT 
GCGTCGCCCA CGGACACAAG CAATTTTGAC AGTTTCCCTG AGGACAGCGA CGAGCCACCG 
CCTGATGACA ACTCAGGCTG GGACATAGAC TTCTAA