Pfkfb4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pfkfb4
Entrez Gene ID	54283
Full Name	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
General protein information	Preferred Names 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 Names 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 6PF-2-K/Fru-2,6-P2ASE testis-type isozyme 6PF-2-K/Fru-2,6-P2ase 4 PFK/FBPase 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q32
Summary	has both fructose-2,6-bisphosphate 2-phosphatase and 6-phosphofructo-2-kinase activities; plays a role in fructose 2,6 bisphosphate metabolism and regulation of glycolysis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006243840.3	XP_006243902.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 4 isoform X2
XM_017595868.1	XP_017451357.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 4 isoform X3
NM_019333.1	NP_062206.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 4
XM_008766563.2	XP_008764785.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 4 isoform X1
XM_006243842.3	XP_006243904.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 4 isoform X3

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pfkfb4	NP_001032454.1
Mus musculus (house mouse)	Pfkfb4	NP_766607.3
Rattus norvegicus (Norway rat)	Pfkfb4	NP_062206.1
Oryza sativa (rice)	Os05g0164100	NP_001054740.1
Arabidopsis thaliana (thale cress)	F2KP	NP_172191.1
Gallus gallus (chicken)	LOC100859653	XP_004944449.1
Xenopus tropicalis (tropical clawed frog)	pfkfb4	NP_998831.1
Homo sapiens (human)	PFKFB4	NP_004558.1
Pan troglodytes (chimpanzee)	PFKFB4	XP_001158394.1
	LOC709854	XP_001112503.2
Canis lupus familiaris (dog)	PFKFB4	XP_541893.2
Bos taurus (cattle)	PFKFB4	NP_001179764.1
Danio rerio (zebrafish)	pfkfb4l	NP_942111.1

Gene Ontology
Bibliography

Related articles in PubMed

Role of Akt/PKB and PFKFB isoenzymes in the control of glycolysis, cell proliferation and protein synthesis in mitogen-stimulated thymocytes.
Houddane A, Bultot L, Novellasdemunt L, Johanns M, Gueuning MA, Vertommen D, Coulie PG, Bartrons R, Hue L, Rider MH
Cellular signalling3423-37(2017 Jun)

Switches in 6-phosphofructo-2-kinase isoenzyme expression during rat sperm maturation.
G?mez M, Navarro-Sabat? A, Manzano A, Duran J, Obach M, Bartrons R
Biochemical and biophysical research communications387(2)330-5(2009 Sep)

The kinase activity of human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase is regulated via inhibition by phosphoenolpyruvate.
Manes NP, El-Maghrabi MR
Archives of biochemistry and biophysics438(2)125-36(2005 Jun)

6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase: head-to-head with a bifunctional enzyme that controls glycolysis.
Rider MH, Bertrand L, Vertommen D, Michels PA, Rousseau GG, Hue L
The Biochemical journal381(Pt 3)561-79(2004 Aug)

Cloning, expression and chromosomal localization of a human testis 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene.
Manzano A, P?rez JX, Nadal M, Estivill X, Lange A, Bartrons R
Gene229(1-2)83-9(1999 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Increased PFKFB4 expression, but not increased PFKFB3 Ser461 phosphorylation, plays a role in increasing glycolysis in mitogen-stimulated thymocytes.
Title: Role of Akt/PKB and PFKFB isoenzymes in the control of glycolysis, cell proliferation and protein synthesis in mitogen-stimulated thymocytes.

The maximum expression of Pfkfb3 was detected before puberty, while that of Pfkfb4 was observed in adulthood during rat sperm maturation.
Title: Switches in 6-phosphofructo-2-kinase isoenzyme expression during rat sperm maturation.

The following Pfkfb4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pfkfb4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa04447	NM_019333.1 Latest version!	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (Pfkfb4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa31623	XM_006243840.3 Latest version!	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (Pfkfb4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa31624	XM_017595868.1 Latest version!	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (Pfkfb4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa31624	XM_006243842.3 Latest version!	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (Pfkfb4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa43417	XM_008766563.2 Latest version!	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (Pfkfb4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pfkfb4 ( NM_019333.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062206.1 Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (Pfkfb4), mRNA.

Price & Availability↑

CloneID	ORa04447
Clone ID Related Accession (Same CDS sequence)	NM_019333.1
Accession Version	NM_019333.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M64797.1. ##Evidence-Data-START## Transcript exon combination :: M64797.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02642708, SAMN02642712 [ECO:0000348] ##Evidence-Data-END##

ATGGCGTCCC CACGGGAACT GACCCAGAAT CCCCTGAAGA AGATCTGGAT GCCATACAGC 
AATGGGAGGC CCGCCCTGCA CGCCAGCCAA CGTGGTGTCT GCATGACCAA CTGCCCGACA 
CTCATCGTCA TGGTGGGCCT GCCAGCCAGG GGCAAGACCT ACATTTCTAA GAAGCTGACC 
CGGTACCTCA ACTGGATTGG CGTGCCTACT CGGGAATTCA ACGTGGGTCA GTATCGCCGG 
GACATGGTCA AGACATACAA ATCTTTTGAG TTCTTCCTCC CAGACAATGA AGAGGGCCTG 
AAAATCAGGA AGCAGTGTGC CTTGGCAGCC CTCAATGACG TCCGGAAGTT CCTCAGTGAG 
GAGGGGGGAC ACGTGGCGGT TTTTGATGCT ACCAATACCA CCCGAGAACG GAGAGCGATG 
ATCTTTAACT TTGGAGAACA GAATGGCTAT AAGACCTTCT TTGTTGAATC TATCTGTGTG 
GATCCTGAGG TCATTGCTGC CAACATCGTG CAAGTGAAGC TGGGCAGCCC TGACTATGTG 
AACCGGGACA GCGACGAAGC CACTGAGGAC TTCATGAGGC GCATTGAATG CTATGAAAAC 
TCCTATGAGT CTCTGGATGA AGAACAGGAC AGGGATCTGT CCTACATCAA GATCATGGAC 
GTGGGGCAGA GTTATGTGGT GAACCGTGTT GCCGACCACA TCCAGAGTCG CATCGTTTAT 
TACCTCATGA ACATCCATGT GACACCCCGC TCCATCTACC TCTGCCGGCA CGGGGAGAGT 
GAGCTCAACC TCAAGGGCCG GATTGGTGGG GACCCTGGAC TCTCCCCCCG GGGCCGGGAG 
TTTTCCAAGC ACCTAGCTCA GTTCATCAGT GACCAGAACA TCAAGGACCT GAAGGTCTGG 
ACGAGCCAGA TGAAGAGGAC GATCCAGACA GCCGAGGCGC TGAGTGTCCC TTATGAGCAG 
TGGAAGGTCC TCAACGAGAT TGACGCGGGT GTCTGTGAGG AAATGACCTA CGAAGAAATC 
CAGGACCACT ACCCGCTGGA GTTTGCTCTG AGGGACCAGG ACAAGTACCG GTACCGCTAC 
CCGAAGGGCG AGTCCTATGA AGACCTGGTG CAGCGGCTGG AGCCTGTCAT CATGGAGCTG 
GAGAGGCAGG AGAACGTGCT AGTCATTTGC CACCAGGCTG TGATGCGTTG CCTCCTGGCC 
TACTTCCTTG ACAAGGCAGC AGAAGAGCTG CCCTACCTCA AATGCCCCTT GCACACAGTC 
CTGAAGCTCA CACCTGTGGC TTACGGTTGT AAAGTGGAGT CCATATTCCT GAATGTGGCA 
GCTGTGAACA CACACCGAGA CAGACCTCAG AATGTAGACA TATCCAGGCC TTCAGAGGAA 
GCCCTTGTCA CAGTTCCTGC TCACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062206.1
CDS	35..1444
Translation	MASPRELTQNPLKKIWMPYSNGRPALHASQRGVCMTNCPTLIVMVGLPARGKTYISKKLTRYLNWIGVPTREFNVGQYRRDMVKTYKSFEFFLPDNEEGLKIRKQCALAALNDVRKFLSEEGGHVAVFDATNTTRERRAMIFNFGEQNGYKTFFVESICVDPEVIAANIVQVKLGSPDYVNRDSDEATEDFMRRIECYENSYESLDEEQDRDLSYIKIMDVGQSYVVNRVADHIQSRIVYYLMNIHVTPRSIYLCRHGESELNLKGRIGGDPGLSPRGREFSKHLAQFISDQNIKDLKVWTSQMKRTIQTAEALSVPYEQWKVLNEIDAGVCEEMTYEEIQDHYPLEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKAAEELPYLKCPLHTVLKLTPVAYGCKVESIFLNVAAVNTHRDRPQNVDISRPSEEALVTVPAHQ

Target ORF information:

RefSeq Version	NM_019333.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (Pfkfb4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019333.1

ORF Insert Sequence:

ATGGCGTCCC CACGGGAACT GACCCAGAAT CCCCTGAAGA AGATCTGGAT GCCATACAGC 
AATGGGAGGC CCGCCCTGCA CGCCAGCCAA CGTGGTGTCT GCATGACCAA CTGCCCGACA 
CTCATCGTCA TGGTGGGCCT GCCAGCCAGG GGCAAGACCT ACATTTCTAA GAAGCTGACC 
CGGTACCTCA ACTGGATTGG CGTGCCTACT CGGGAATTCA ACGTGGGTCA GTATCGCCGG 
GACATGGTCA AGACATACAA ATCTTTTGAG TTCTTCCTCC CAGACAATGA AGAGGGCCTG 
AAAATCAGGA AGCAGTGTGC CTTGGCAGCC CTCAATGACG TCCGGAAGTT CCTCAGTGAG 
GAGGGGGGAC ACGTGGCGGT TTTTGATGCT ACCAATACCA CCCGAGAACG GAGAGCGATG 
ATCTTTAACT TTGGAGAACA GAATGGCTAT AAGACCTTCT TTGTTGAATC TATCTGTGTG 
GATCCTGAGG TCATTGCTGC CAACATCGTG CAAGTGAAGC TGGGCAGCCC TGACTATGTG 
AACCGGGACA GCGACGAAGC CACTGAGGAC TTCATGAGGC GCATTGAATG CTATGAAAAC 
TCCTATGAGT CTCTGGATGA AGAACAGGAC AGGGATCTGT CCTACATCAA GATCATGGAC 
GTGGGGCAGA GTTATGTGGT GAACCGTGTT GCCGACCACA TCCAGAGTCG CATCGTTTAT 
TACCTCATGA ACATCCATGT GACACCCCGC TCCATCTACC TCTGCCGGCA CGGGGAGAGT 
GAGCTCAACC TCAAGGGCCG GATTGGTGGG GACCCTGGAC TCTCCCCCCG GGGCCGGGAG 
TTTTCCAAGC ACCTAGCTCA GTTCATCAGT GACCAGAACA TCAAGGACCT GAAGGTCTGG 
ACGAGCCAGA TGAAGAGGAC GATCCAGACA GCCGAGGCGC TGAGTGTCCC TTATGAGCAG 
TGGAAGGTCC TCAACGAGAT TGACGCGGGT GTCTGTGAGG AAATGACCTA CGAAGAAATC 
CAGGACCACT ACCCGCTGGA GTTTGCTCTG AGGGACCAGG ACAAGTACCG GTACCGCTAC 
CCGAAGGGCG AGTCCTATGA AGACCTGGTG CAGCGGCTGG AGCCTGTCAT CATGGAGCTG 
GAGAGGCAGG AGAACGTGCT AGTCATTTGC CACCAGGCTG TGATGCGTTG CCTCCTGGCC 
TACTTCCTTG ACAAGGCAGC AGAAGAGCTG CCCTACCTCA AATGCCCCTT GCACACAGTC 
CTGAAGCTCA CACCTGTGGC TTACGGTTGT AAAGTGGAGT CCATATTCCT GAATGTGGCA 
GCTGTGAACA CACACCGAGA CAGACCTCAG AATGTAGACA TATCCAGGCC TTCAGAGGAA 
GCCCTTGTCA CAGTTCCTGC TCACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfkfb4 ( XM_006243840.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243902.1 Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (Pfkfb4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa31623
Clone ID Related Accession (Same CDS sequence)	XM_006243840.3
Accession Version	XM_006243840.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243840.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGAT GGACTTTAGG AGGCACCTCT CTAACCGACC TTTGGCGGGA GAGCCACGGC 
CAGGTCTGCA TGACCAACTG CCCGACACTC ATCGTCATGG TGGGCCTGCC AGCCAGGGGC 
AAGACCTACA TTTCTAAGAA GCTGACCCGG TACCTCAACT GGATTGGCGT GCCTACTCGG 
GAATTCAACG TGGGTCAGTA TCGCCGGGAC ATGGTCAAGA CATACAAATC TTTTGAGTTC 
TTCCTCCCAG ACAATGAAGA GGGCCTGAAA ATCAGGAAGC AGTGTGCCTT GGCAGCCCTC 
AATGACGTCC GGAAGTTCCT CAGTGAGGAG GGGGGACACG TGGCGGTTTT TGATGCTACC 
AATACCACCC GAGAACGGAG AGCGATGATC TTTAACTTTG GAGAACAGAA TGGCTATAAG 
ACCTTCTTTG TTGAATCTAT CTGTGTGGAT CCTGAGGTCA TTGCTGCCAA CATCGTGCAA 
GTGAAGCTGG GCAGCCCTGA CTATGTGAAC CGGGACAGCG ACGAAGCCAC TGAGGACTTC 
ATGAGGCGCA TTGAATGCTA TGAAAACTCC TATGAGTCTC TGGATGAAGA ACAGGACAGG 
GATCTGTCCT ACATCAAGAT CATGGACGTG GGGCAGAGTT ATGTGGTGAA CCGTGTTGCC 
GACCACATCC AGAGTCGCAT CGTTTATTAC CTCATGAACA TCCATGTAAC ACCCCGCTCC 
ATCTACCTCT GCCGGCACGG GGAGAGTGAG CTCAACCTCA AGGGCCGGAT TGGTGGGGAC 
CCTGGACTCT CCCCCCGGGG CCGGGAGTTT TCCAAGCACC TAGCTCAGTT CATCAGTGAC 
CAGAACATCA AGGACCTGAA GGTCTGGACG AGCCAGATGA AGAGGACGAT CCAGACAGCC 
GAGGCGCTGA GTGTCCCTTA TGAGCAGTGG AAGGTCCTCA ACGAGATTGA CGCGGGTGTC 
TGTGAGGAAA TGACCTACGA AGAAATCCAG GACCACTACC CGCTGGAGTT TGCTCTGAGG 
GACCAGGACA AGTACCGGTA CCGCTACCCG AAGGGCGAGT CCTATGAAGA CCTGGTGCAG 
CGGCTGGAGC CTGTCATCAT GGAGCTGGAG AGGCAGGAGA ACGTGCTAGT CATTTGCCAC 
CAGGCTGTGA TGCGTTGCCT CCTGGCCTAC TTCCTTGACA AGGCAGCAGA AGAGCTGCCC 
TACCTCAAAT GCCCCTTGCA CACAGTCCTG AAGCTCACAC CTGTGGCTTA CGGTTGTAAA 
GTGGAGTCCA TATTCCTGAA TGTGGCAGCT GTGAACACAC ACCGAGACAG ACCTCAGAAT 
GTAGACATAT CCAGGCCTTC AGAGGAAGCC CTTGTCACAG TTCCTGCTCA CCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243902.1
CDS	35..1411
Translation	MDRWTLGGTSLTDLWRESHGQVCMTNCPTLIVMVGLPARGKTYISKKLTRYLNWIGVPTREFNVGQYRRDMVKTYKSFEFFLPDNEEGLKIRKQCALAALNDVRKFLSEEGGHVAVFDATNTTRERRAMIFNFGEQNGYKTFFVESICVDPEVIAANIVQVKLGSPDYVNRDSDEATEDFMRRIECYENSYESLDEEQDRDLSYIKIMDVGQSYVVNRVADHIQSRIVYYLMNIHVTPRSIYLCRHGESELNLKGRIGGDPGLSPRGREFSKHLAQFISDQNIKDLKVWTSQMKRTIQTAEALSVPYEQWKVLNEIDAGVCEEMTYEEIQDHYPLEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKAAEELPYLKCPLHTVLKLTPVAYGCKVESIFLNVAAVNTHRDRPQNVDISRPSEEALVTVPAHQ

Target ORF information:

RefSeq Version	XM_006243840.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (Pfkfb4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243840.3

ORF Insert Sequence:

ATGGACAGAT GGACTTTAGG AGGCACCTCT CTAACCGACC TTTGGCGGGA GAGCCACGGC 
CAGGTCTGCA TGACCAACTG CCCGACACTC ATCGTCATGG TGGGCCTGCC AGCCAGGGGC 
AAGACCTACA TTTCTAAGAA GCTGACCCGG TACCTCAACT GGATTGGCGT GCCTACTCGG 
GAATTCAACG TGGGTCAGTA TCGCCGGGAC ATGGTCAAGA CATACAAATC TTTTGAGTTC 
TTCCTCCCAG ACAATGAAGA GGGCCTGAAA ATCAGGAAGC AGTGTGCCTT GGCAGCCCTC 
AATGACGTCC GGAAGTTCCT CAGTGAGGAG GGGGGACACG TGGCGGTTTT TGATGCTACC 
AATACCACCC GAGAACGGAG AGCGATGATC TTTAACTTTG GAGAACAGAA TGGCTATAAG 
ACCTTCTTTG TTGAATCTAT CTGTGTGGAT CCTGAGGTCA TTGCTGCCAA CATCGTGCAA 
GTGAAGCTGG GCAGCCCTGA CTATGTGAAC CGGGACAGCG ACGAAGCCAC TGAGGACTTC 
ATGAGGCGCA TTGAATGCTA TGAAAACTCC TATGAGTCTC TGGATGAAGA ACAGGACAGG 
GATCTGTCCT ACATCAAGAT CATGGACGTG GGGCAGAGTT ATGTGGTGAA CCGTGTTGCC 
GACCACATCC AGAGTCGCAT CGTTTATTAC CTCATGAACA TCCATGTAAC ACCCCGCTCC 
ATCTACCTCT GCCGGCACGG GGAGAGTGAG CTCAACCTCA AGGGCCGGAT TGGTGGGGAC 
CCTGGACTCT CCCCCCGGGG CCGGGAGTTT TCCAAGCACC TAGCTCAGTT CATCAGTGAC 
CAGAACATCA AGGACCTGAA GGTCTGGACG AGCCAGATGA AGAGGACGAT CCAGACAGCC 
GAGGCGCTGA GTGTCCCTTA TGAGCAGTGG AAGGTCCTCA ACGAGATTGA CGCGGGTGTC 
TGTGAGGAAA TGACCTACGA AGAAATCCAG GACCACTACC CGCTGGAGTT TGCTCTGAGG 
GACCAGGACA AGTACCGGTA CCGCTACCCG AAGGGCGAGT CCTATGAAGA CCTGGTGCAG 
CGGCTGGAGC CTGTCATCAT GGAGCTGGAG AGGCAGGAGA ACGTGCTAGT CATTTGCCAC 
CAGGCTGTGA TGCGTTGCCT CCTGGCCTAC TTCCTTGACA AGGCAGCAGA AGAGCTGCCC 
TACCTCAAAT GCCCCTTGCA CACAGTCCTG AAGCTCACAC CTGTGGCTTA CGGTTGTAAA 
GTGGAGTCCA TATTCCTGAA TGTGGCAGCT GTGAACACAC ACCGAGACAG ACCTCAGAAT 
GTAGACATAT CCAGGCCTTC AGAGGAAGCC CTTGTCACAG TTCCTGCTCA CCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfkfb4 ( XM_017595868.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017451357.1 Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (Pfkfb4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa31624
Clone ID Related Accession (Same CDS sequence)	XM_017595868.1 , XM_006243842.3
Accession Version	XM_017595868.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAACT GCCCGACACT CATCGTCATG GTGGGCCTGC CAGCCAGGGG CAAGACCTAC 
ATTTCTAAGA AGCTGACCCG GTACCTCAAC TGGATTGGCG TGCCTACTCG GGAATTCAAC 
GTGGGTCAGT ATCGCCGGGA CATGGTCAAG ACATACAAAT CTTTTGAGTT CTTCCTCCCA 
GACAATGAAG AGGGCCTGAA AATCAGGAAG CAGTGTGCCT TGGCAGCCCT CAATGACGTC 
CGGAAGTTCC TCAGTGAGGA GGGGGGACAC GTGGCGGTTT TTGATGCTAC CAATACCACC 
CGAGAACGGA GAGCGATGAT CTTTAACTTT GGAGAACAGA ATGGCTATAA GACCTTCTTT 
GTTGAATCTA TCTGTGTGGA TCCTGAGGTC ATTGCTGCCA ACATCGTGCA AGTGAAGCTG 
GGCAGCCCTG ACTATGTGAA CCGGGACAGC GACGAAGCCA CTGAGGACTT CATGAGGCGC 
ATTGAATGCT ATGAAAACTC CTATGAGTCT CTGGATGAAG AACAGGACAG GGATCTGTCC 
TACATCAAGA TCATGGACGT GGGGCAGAGT TATGTGGTGA ACCGTGTTGC CGACCACATC 
CAGAGTCGCA TCGTTTATTA CCTCATGAAC ATCCATGTAA CACCCCGCTC CATCTACCTC 
TGCCGGCACG GGGAGAGTGA GCTCAACCTC AAGGGCCGGA TTGGTGGGGA CCCTGGACTC 
TCCCCCCGGG GCCGGGAGTT TTCCAAGCAC CTAGCTCAGT TCATCAGTGA CCAGAACATC 
AAGGACCTGA AGGTCTGGAC GAGCCAGATG AAGAGGACGA TCCAGACAGC CGAGGCGCTG 
AGTGTCCCTT ATGAGCAGTG GAAGGTCCTC AACGAGATTG ACGCGGGTGT CTGTGAGGAA 
ATGACCTACG AAGAAATCCA GGACCACTAC CCGCTGGAGT TTGCTCTGAG GGACCAGGAC 
AAGTACCGGT ACCGCTACCC GAAGGGCGAG TCCTATGAAG ACCTGGTGCA GCGGCTGGAG 
CCTGTCATCA TGGAGCTGGA GAGGCAGGAG AACGTGCTAG TCATTTGCCA CCAGGCTGTG 
ATGCGTTGCC TCCTGGCCTA CTTCCTTGAC AAGGCAGCAG AAGAGCTGCC CTACCTCAAA 
TGCCCCTTGC ACACAGTCCT GAAGCTCACA CCTGTGGCTT ACGGTTGTAA AGTGGAGTCC 
ATATTCCTGA ATGTGGCAGC TGTGAACACA CACCGAGACA GACCTCAGAA TGTAGACATA 
TCCAGGCCTT CAGAGGAAGC CCTTGTCACA GTTCCTGCTC ACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017451357.1
CDS	285..1592
Translation	MTNCPTLIVMVGLPARGKTYISKKLTRYLNWIGVPTREFNVGQYRRDMVKTYKSFEFFLPDNEEGLKIRKQCALAALNDVRKFLSEEGGHVAVFDATNTTRERRAMIFNFGEQNGYKTFFVESICVDPEVIAANIVQVKLGSPDYVNRDSDEATEDFMRRIECYENSYESLDEEQDRDLSYIKIMDVGQSYVVNRVADHIQSRIVYYLMNIHVTPRSIYLCRHGESELNLKGRIGGDPGLSPRGREFSKHLAQFISDQNIKDLKVWTSQMKRTIQTAEALSVPYEQWKVLNEIDAGVCEEMTYEEIQDHYPLEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKAAEELPYLKCPLHTVLKLTPVAYGCKVESIFLNVAAVNTHRDRPQNVDISRPSEEALVTVPAHQ

Target ORF information:

RefSeq Version	XM_017595868.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (Pfkfb4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595868.1

ORF Insert Sequence:

ATGACCAACT GCCCGACACT CATCGTCATG GTGGGCCTGC CAGCCAGGGG CAAGACCTAC 
ATTTCTAAGA AGCTGACCCG GTACCTCAAC TGGATTGGCG TGCCTACTCG GGAATTCAAC 
GTGGGTCAGT ATCGCCGGGA CATGGTCAAG ACATACAAAT CTTTTGAGTT CTTCCTCCCA 
GACAATGAAG AGGGCCTGAA AATCAGGAAG CAGTGTGCCT TGGCAGCCCT CAATGACGTC 
CGGAAGTTCC TCAGTGAGGA GGGGGGACAC GTGGCGGTTT TTGATGCTAC CAATACCACC 
CGAGAACGGA GAGCGATGAT CTTTAACTTT GGAGAACAGA ATGGCTATAA GACCTTCTTT 
GTTGAATCTA TCTGTGTGGA TCCTGAGGTC ATTGCTGCCA ACATCGTGCA AGTGAAGCTG 
GGCAGCCCTG ACTATGTGAA CCGGGACAGC GACGAAGCCA CTGAGGACTT CATGAGGCGC 
ATTGAATGCT ATGAAAACTC CTATGAGTCT CTGGATGAAG AACAGGACAG GGATCTGTCC 
TACATCAAGA TCATGGACGT GGGGCAGAGT TATGTGGTGA ACCGTGTTGC CGACCACATC 
CAGAGTCGCA TCGTTTATTA CCTCATGAAC ATCCATGTAA CACCCCGCTC CATCTACCTC 
TGCCGGCACG GGGAGAGTGA GCTCAACCTC AAGGGCCGGA TTGGTGGGGA CCCTGGACTC 
TCCCCCCGGG GCCGGGAGTT TTCCAAGCAC CTAGCTCAGT TCATCAGTGA CCAGAACATC 
AAGGACCTGA AGGTCTGGAC GAGCCAGATG AAGAGGACGA TCCAGACAGC CGAGGCGCTG 
AGTGTCCCTT ATGAGCAGTG GAAGGTCCTC AACGAGATTG ACGCGGGTGT CTGTGAGGAA 
ATGACCTACG AAGAAATCCA GGACCACTAC CCGCTGGAGT TTGCTCTGAG GGACCAGGAC 
AAGTACCGGT ACCGCTACCC GAAGGGCGAG TCCTATGAAG ACCTGGTGCA GCGGCTGGAG 
CCTGTCATCA TGGAGCTGGA GAGGCAGGAG AACGTGCTAG TCATTTGCCA CCAGGCTGTG 
ATGCGTTGCC TCCTGGCCTA CTTCCTTGAC AAGGCAGCAG AAGAGCTGCC CTACCTCAAA 
TGCCCCTTGC ACACAGTCCT GAAGCTCACA CCTGTGGCTT ACGGTTGTAA AGTGGAGTCC 
ATATTCCTGA ATGTGGCAGC TGTGAACACA CACCGAGACA GACCTCAGAA TGTAGACATA 
TCCAGGCCTT CAGAGGAAGC CCTTGTCACA GTTCCTGCTC ACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfkfb4 ( XM_006243842.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243904.1 Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (Pfkfb4), transcript variant X3, mRNA.

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CloneID	ORa31624
Clone ID Related Accession (Same CDS sequence)	XM_017595868.1 , XM_006243842.3
Accession Version	XM_006243842.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243842.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAACT GCCCGACACT CATCGTCATG GTGGGCCTGC CAGCCAGGGG CAAGACCTAC 
ATTTCTAAGA AGCTGACCCG GTACCTCAAC TGGATTGGCG TGCCTACTCG GGAATTCAAC 
GTGGGTCAGT ATCGCCGGGA CATGGTCAAG ACATACAAAT CTTTTGAGTT CTTCCTCCCA 
GACAATGAAG AGGGCCTGAA AATCAGGAAG CAGTGTGCCT TGGCAGCCCT CAATGACGTC 
CGGAAGTTCC TCAGTGAGGA GGGGGGACAC GTGGCGGTTT TTGATGCTAC CAATACCACC 
CGAGAACGGA GAGCGATGAT CTTTAACTTT GGAGAACAGA ATGGCTATAA GACCTTCTTT 
GTTGAATCTA TCTGTGTGGA TCCTGAGGTC ATTGCTGCCA ACATCGTGCA AGTGAAGCTG 
GGCAGCCCTG ACTATGTGAA CCGGGACAGC GACGAAGCCA CTGAGGACTT CATGAGGCGC 
ATTGAATGCT ATGAAAACTC CTATGAGTCT CTGGATGAAG AACAGGACAG GGATCTGTCC 
TACATCAAGA TCATGGACGT GGGGCAGAGT TATGTGGTGA ACCGTGTTGC CGACCACATC 
CAGAGTCGCA TCGTTTATTA CCTCATGAAC ATCCATGTAA CACCCCGCTC CATCTACCTC 
TGCCGGCACG GGGAGAGTGA GCTCAACCTC AAGGGCCGGA TTGGTGGGGA CCCTGGACTC 
TCCCCCCGGG GCCGGGAGTT TTCCAAGCAC CTAGCTCAGT TCATCAGTGA CCAGAACATC 
AAGGACCTGA AGGTCTGGAC GAGCCAGATG AAGAGGACGA TCCAGACAGC CGAGGCGCTG 
AGTGTCCCTT ATGAGCAGTG GAAGGTCCTC AACGAGATTG ACGCGGGTGT CTGTGAGGAA 
ATGACCTACG AAGAAATCCA GGACCACTAC CCGCTGGAGT TTGCTCTGAG GGACCAGGAC 
AAGTACCGGT ACCGCTACCC GAAGGGCGAG TCCTATGAAG ACCTGGTGCA GCGGCTGGAG 
CCTGTCATCA TGGAGCTGGA GAGGCAGGAG AACGTGCTAG TCATTTGCCA CCAGGCTGTG 
ATGCGTTGCC TCCTGGCCTA CTTCCTTGAC AAGGCAGCAG AAGAGCTGCC CTACCTCAAA 
TGCCCCTTGC ACACAGTCCT GAAGCTCACA CCTGTGGCTT ACGGTTGTAA AGTGGAGTCC 
ATATTCCTGA ATGTGGCAGC TGTGAACACA CACCGAGACA GACCTCAGAA TGTAGACATA 
TCCAGGCCTT CAGAGGAAGC CCTTGTCACA GTTCCTGCTC ACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243904.1
CDS	168..1475
Translation	MTNCPTLIVMVGLPARGKTYISKKLTRYLNWIGVPTREFNVGQYRRDMVKTYKSFEFFLPDNEEGLKIRKQCALAALNDVRKFLSEEGGHVAVFDATNTTRERRAMIFNFGEQNGYKTFFVESICVDPEVIAANIVQVKLGSPDYVNRDSDEATEDFMRRIECYENSYESLDEEQDRDLSYIKIMDVGQSYVVNRVADHIQSRIVYYLMNIHVTPRSIYLCRHGESELNLKGRIGGDPGLSPRGREFSKHLAQFISDQNIKDLKVWTSQMKRTIQTAEALSVPYEQWKVLNEIDAGVCEEMTYEEIQDHYPLEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKAAEELPYLKCPLHTVLKLTPVAYGCKVESIFLNVAAVNTHRDRPQNVDISRPSEEALVTVPAHQ

Target ORF information:

RefSeq Version	XM_006243842.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (Pfkfb4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243842.3

ORF Insert Sequence:

ATGACCAACT GCCCGACACT CATCGTCATG GTGGGCCTGC CAGCCAGGGG CAAGACCTAC 
ATTTCTAAGA AGCTGACCCG GTACCTCAAC TGGATTGGCG TGCCTACTCG GGAATTCAAC 
GTGGGTCAGT ATCGCCGGGA CATGGTCAAG ACATACAAAT CTTTTGAGTT CTTCCTCCCA 
GACAATGAAG AGGGCCTGAA AATCAGGAAG CAGTGTGCCT TGGCAGCCCT CAATGACGTC 
CGGAAGTTCC TCAGTGAGGA GGGGGGACAC GTGGCGGTTT TTGATGCTAC CAATACCACC 
CGAGAACGGA GAGCGATGAT CTTTAACTTT GGAGAACAGA ATGGCTATAA GACCTTCTTT 
GTTGAATCTA TCTGTGTGGA TCCTGAGGTC ATTGCTGCCA ACATCGTGCA AGTGAAGCTG 
GGCAGCCCTG ACTATGTGAA CCGGGACAGC GACGAAGCCA CTGAGGACTT CATGAGGCGC 
ATTGAATGCT ATGAAAACTC CTATGAGTCT CTGGATGAAG AACAGGACAG GGATCTGTCC 
TACATCAAGA TCATGGACGT GGGGCAGAGT TATGTGGTGA ACCGTGTTGC CGACCACATC 
CAGAGTCGCA TCGTTTATTA CCTCATGAAC ATCCATGTAA CACCCCGCTC CATCTACCTC 
TGCCGGCACG GGGAGAGTGA GCTCAACCTC AAGGGCCGGA TTGGTGGGGA CCCTGGACTC 
TCCCCCCGGG GCCGGGAGTT TTCCAAGCAC CTAGCTCAGT TCATCAGTGA CCAGAACATC 
AAGGACCTGA AGGTCTGGAC GAGCCAGATG AAGAGGACGA TCCAGACAGC CGAGGCGCTG 
AGTGTCCCTT ATGAGCAGTG GAAGGTCCTC AACGAGATTG ACGCGGGTGT CTGTGAGGAA 
ATGACCTACG AAGAAATCCA GGACCACTAC CCGCTGGAGT TTGCTCTGAG GGACCAGGAC 
AAGTACCGGT ACCGCTACCC GAAGGGCGAG TCCTATGAAG ACCTGGTGCA GCGGCTGGAG 
CCTGTCATCA TGGAGCTGGA GAGGCAGGAG AACGTGCTAG TCATTTGCCA CCAGGCTGTG 
ATGCGTTGCC TCCTGGCCTA CTTCCTTGAC AAGGCAGCAG AAGAGCTGCC CTACCTCAAA 
TGCCCCTTGC ACACAGTCCT GAAGCTCACA CCTGTGGCTT ACGGTTGTAA AGTGGAGTCC 
ATATTCCTGA ATGTGGCAGC TGTGAACACA CACCGAGACA GACCTCAGAA TGTAGACATA 
TCCAGGCCTT CAGAGGAAGC CCTTGTCACA GTTCCTGCTC ACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfkfb4 ( XM_008766563.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764785.1 Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (Pfkfb4), transcript variant X1, mRNA.

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CloneID	ORa43417
Clone ID Related Accession (Same CDS sequence)	XM_008766563.2
Accession Version	XM_008766563.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008766563.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCC CACGGGAACT GACCCAGAAT CCCCTGAAGA AGATCTGGAT GCCATACAGC 
AATGGGAGGC CCGCCCTGCA CGCCAGCCAA CGTGGTGTCT GCATGACCAA CTGCCCGACA 
CTCATCGTCA TGGTGGGCCT GCCAGCCAGG GGCAAGACCT ACATTTCTAA GAAGCTGACC 
CGGTACCTCA ACTGGATTGG CGTGCCTACT CGGGAATTCA ACGTGGGTCA GTATCGCCGG 
GACATGGTCA AGACATACAA ATCTTTTGAG TTCTTCCTCC CAGACAATGA AGAGGGCCTG 
AAAATCAGGA AGCAGTGTGC CTTGGCAGCC CTCAATGACG TCCGGAAGTT CCTCAGTGAG 
GAGGGGGGAC ACGTGGCGGT TTTTGATGCT ACCAATACCA CCCGAGAACG GAGAGCGATG 
ATCTTTAACT TTGGAGAACA GAATGGCTAT AAGACCTTCT TTGTTGAATC TATCTGTGTG 
GATCCTGAGG TCATTGCTGC CAACATCGTG CAAGTGAAGC TGGGCAGCCC TGACTATGTG 
AACCGGGACA GCGACGAAGC CACTGAGGAC TTCATGAGGC GCATTGAATG CTATGAAAAC 
TCCTATGAGT CTCTGGATGA AGAACAGGAC AGGGATCTGT CCTACATCAA GATCATGGAC 
GTGGGGCAGA GTTATGTGGT GAACCGTGTT GCCGACCACA TCCAGAGTCG CATCGTTTAT 
TACCTCATGA ACATCCATGT AACACCCCGC TCCATCTACC TCTGCCGGCA CGGGGAGAGT 
GAGCTCAACC TCAAGGGCCG GATTGGTGGG GACCCTGGAC TCTCCCCCCG GGGCCGGGAG 
TTTTCCAAGC ACCTAGCTCA GTTCATCAGT GACCAGAACA TCAAGGACCT GAAGGTCTGG 
ACGAGCCAGA TGAAGAGGAC GATCCAGACA GCCGAGGCGC TGAGTGTCCC TTATGAGCAG 
TGGAAGGTCC TCAACGAGAT TGACGCGGGT GTCTGTGAGG AAATGACCTA CGAAGAAATC 
CAGGACCACT ACCCGCTGGA GTTTGCTCTG AGGGACCAGG ACAAGTACCG GTACCGCTAC 
CCGAAGGGCG AGTCCTATGA AGACCTGGTG CAGCGGCTGG AGCCTGTCAT CATGGAGCTG 
GAGAGGCAGG AGAACGTGCT AGTCATTTGC CACCAGGCTG TGATGCGTTG CCTCCTGGCC 
TACTTCCTTG ACAAGGCAGC AGAAGAGCTG CCCTACCTCA AATGCCCCTT GCACACAGTC 
CTGAAGCTCA CACCTGTGGC TTACGGTTGT AAAGTGGAGT CCATATTCCT GAATGTGGCA 
GCTGTGAACA CACACCGAGA CAGACCTCAG AATGTAGACA TATCCAGGCC TTCAGAGGAA 
GCCCTTGTCA CAGTTCCTGC TCACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764785.1
CDS	365..1774
Translation	MASPRELTQNPLKKIWMPYSNGRPALHASQRGVCMTNCPTLIVMVGLPARGKTYISKKLTRYLNWIGVPTREFNVGQYRRDMVKTYKSFEFFLPDNEEGLKIRKQCALAALNDVRKFLSEEGGHVAVFDATNTTRERRAMIFNFGEQNGYKTFFVESICVDPEVIAANIVQVKLGSPDYVNRDSDEATEDFMRRIECYENSYESLDEEQDRDLSYIKIMDVGQSYVVNRVADHIQSRIVYYLMNIHVTPRSIYLCRHGESELNLKGRIGGDPGLSPRGREFSKHLAQFISDQNIKDLKVWTSQMKRTIQTAEALSVPYEQWKVLNEIDAGVCEEMTYEEIQDHYPLEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKAAEELPYLKCPLHTVLKLTPVAYGCKVESIFLNVAAVNTHRDRPQNVDISRPSEEALVTVPAHQ

Target ORF information:

RefSeq Version	XM_008766563.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (Pfkfb4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008766563.2

ORF Insert Sequence:

ATGGCGTCCC CACGGGAACT GACCCAGAAT CCCCTGAAGA AGATCTGGAT GCCATACAGC 
AATGGGAGGC CCGCCCTGCA CGCCAGCCAA CGTGGTGTCT GCATGACCAA CTGCCCGACA 
CTCATCGTCA TGGTGGGCCT GCCAGCCAGG GGCAAGACCT ACATTTCTAA GAAGCTGACC 
CGGTACCTCA ACTGGATTGG CGTGCCTACT CGGGAATTCA ACGTGGGTCA GTATCGCCGG 
GACATGGTCA AGACATACAA ATCTTTTGAG TTCTTCCTCC CAGACAATGA AGAGGGCCTG 
AAAATCAGGA AGCAGTGTGC CTTGGCAGCC CTCAATGACG TCCGGAAGTT CCTCAGTGAG 
GAGGGGGGAC ACGTGGCGGT TTTTGATGCT ACCAATACCA CCCGAGAACG GAGAGCGATG 
ATCTTTAACT TTGGAGAACA GAATGGCTAT AAGACCTTCT TTGTTGAATC TATCTGTGTG 
GATCCTGAGG TCATTGCTGC CAACATCGTG CAAGTGAAGC TGGGCAGCCC TGACTATGTG 
AACCGGGACA GCGACGAAGC CACTGAGGAC TTCATGAGGC GCATTGAATG CTATGAAAAC 
TCCTATGAGT CTCTGGATGA AGAACAGGAC AGGGATCTGT CCTACATCAA GATCATGGAC 
GTGGGGCAGA GTTATGTGGT GAACCGTGTT GCCGACCACA TCCAGAGTCG CATCGTTTAT 
TACCTCATGA ACATCCATGT AACACCCCGC TCCATCTACC TCTGCCGGCA CGGGGAGAGT 
GAGCTCAACC TCAAGGGCCG GATTGGTGGG GACCCTGGAC TCTCCCCCCG GGGCCGGGAG 
TTTTCCAAGC ACCTAGCTCA GTTCATCAGT GACCAGAACA TCAAGGACCT GAAGGTCTGG 
ACGAGCCAGA TGAAGAGGAC GATCCAGACA GCCGAGGCGC TGAGTGTCCC TTATGAGCAG 
TGGAAGGTCC TCAACGAGAT TGACGCGGGT GTCTGTGAGG AAATGACCTA CGAAGAAATC 
CAGGACCACT ACCCGCTGGA GTTTGCTCTG AGGGACCAGG ACAAGTACCG GTACCGCTAC 
CCGAAGGGCG AGTCCTATGA AGACCTGGTG CAGCGGCTGG AGCCTGTCAT CATGGAGCTG 
GAGAGGCAGG AGAACGTGCT AGTCATTTGC CACCAGGCTG TGATGCGTTG CCTCCTGGCC 
TACTTCCTTG ACAAGGCAGC AGAAGAGCTG CCCTACCTCA AATGCCCCTT GCACACAGTC 
CTGAAGCTCA CACCTGTGGC TTACGGTTGT AAAGTGGAGT CCATATTCCT GAATGTGGCA 
GCTGTGAACA CACACCGAGA CAGACCTCAG AATGTAGACA TATCCAGGCC TTCAGAGGAA 
GCCCTTGTCA CAGTTCCTGC TCACCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of Akt/PKB and PFKFB isoenzymes in the control of glycolysis, cell proliferation and protein synthesis in mitogen-stimulated thymocytes.
Cellular signalling3423-37(2017 Jun)
Houddane A,Bultot L,Novellasdemunt L,Johanns M,Gueuning MA,Vertommen D,Coulie PG,Bartrons R,Hue L,Rider MH

Switches in 6-phosphofructo-2-kinase isoenzyme expression during rat sperm maturation.
Biochemical and biophysical research communications387(2)330-5(2009 Sep)
G?mez M,Navarro-Sabat? A,Manzano A,Duran J,Obach M,Bartrons R

The kinase activity of human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase is regulated via inhibition by phosphoenolpyruvate.
Archives of biochemistry and biophysics438(2)125-36(2005 Jun)
Manes NP,El-Maghrabi MR

6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase: head-to-head with a bifunctional enzyme that controls glycolysis.
The Biochemical journal381(Pt 3)561-79(2004 Aug)
Rider MH,Bertrand L,Vertommen D,Michels PA,Rousseau GG,Hue L

Cloning, expression and chromosomal localization of a human testis 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene.
Gene229(1-2)83-9(1999 Mar)
Manzano A,P?rez JX,Nadal M,Estivill X,Lange A,Bartrons R