Magi1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Magi1
Entrez Gene ID	500261
Full Name	membrane associated guanylate kinase, WW and PDZ domain containing 1
Synonyms	BAP-1,Baiap1,MAGI-1
General protein information	Preferred Names membrane associated guanylate kinase, WW and PDZ domain containing 1 Names membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 BAI1-associated protein 1 membrane-associated guanylate kinase inverted 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q34

mRNA and Protein(s)

mRNA	Protein	Name
XM_006236927.3	XP_006236989.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X1
XM_006236928.3	XP_006236990.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X3
XM_008763133.2	XP_008761355.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X5
XM_017592832.1	XP_017448321.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X2
XM_006236929.3	XP_006236991.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X4
NM_001030045.1	NP_001025216.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MAGI1	XP_003309903.1
Danio rerio (zebrafish)	magi1b	NP_001007064.1
Mus musculus (house mouse)	Magi1	NP_001025021.1
Gallus gallus (chicken)	MAGI1	XP_003642069.1
Rattus norvegicus (Norway rat)	Magi1	NP_001025216.1
Homo sapiens (human)	MAGI1	NP_001028229.1
Canis lupus familiaris (dog)	MAGI1	XP_005633237.1
Bos taurus (cattle)	MAGI1	XP_002697009.1

Gene Ontology
Bibliography

Related articles in PubMed

Biochemical and morphological characterization of MAGI-1 in neuronal tissue.
Ito H, Morishita R, Sudo K, Nishimura YV, Inaguma Y, Iwamoto I, Nagata K
Journal of neuroscience research90(9)1776-81(2012 Sep)

A genome-wide study reveals rare CNVs exclusive to extreme phenotypes of Alzheimer disease.
Rovelet-Lecrux A, Legallic S, Wallon D, Flaman JM, Martinaud O, Bombois S, Rollin-Sillaire A, Michon A, Le Ber I, Pariente J, Puel M, Paquet C, Croisile B, Thomas-Ant?rion C, Vercelletto M, L?vy R, Fr?bourg T, Hannequin D, Campion D,
European journal of human genetics : EJHG20(6)613-7(2012 Jun)

Regulation of glutamate transporter GLT-1 by MAGI-1.
Zou S, Pita-Almenar JD, Eskin A
Journal of neurochemistry117(5)833-40(2011 Jun)

Interaction of endothelial cell-selective adhesion molecule and MAGI-1 promotes mature cell-cell adhesion via activation of RhoA.
Kimura R, Ishida T, Kuriyama M, Hirata K, Hayashi Y
Genes to cells : devoted to molecular & cellular mechanisms15(4)385-96(2010 Apr)

MAGI-1a functions as a scaffolding protein for the distal renal tubular basolateral K+ channels.
Tanemoto M, Toyohara T, Abe T, Ito S
The Journal of biological chemistry283(18)12241-7(2008 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MAGI-1 was expressed in Purkinje cells, in hypocampal neurons in CA1 region, in the glomerulus region of olfactory bulb, and at the dorsal root entry zone in embryonic rat spinal cord
Title: Biochemical and morphological characterization of MAGI-1 in neuronal tissue.

These data suggest that MAGI-1 regulates surface expression of GLT-1 and the level of glutamate in the hippocampus
Title: Regulation of glutamate transporter GLT-1 by MAGI-1.

These results demonstrate that the phosphorylation-dependent scaffolding of the basolateral K(+) channels by MAGI-1a-long participates in the renal regulation of the fluid and electrolyte homeostasis.
Title: MAGI-1a functions as a scaffolding protein for the distal renal tubular basolateral K+ channels.

The following Magi1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Magi1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa60988	XM_017592832.1 Latest version!	Rattus norvegicus membrane associated guanylate kinase, WW and PDZ domain containing 1 (Magi1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ORa27495	XM_006236927.3 Latest version!	Rattus norvegicus membrane associated guanylate kinase, WW and PDZ domain containing 1 (Magi1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa27496	XM_006236928.3 Latest version!	Rattus norvegicus membrane associated guanylate kinase, WW and PDZ domain containing 1 (Magi1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa27497	XM_006236929.3 Latest version!	Rattus norvegicus membrane associated guanylate kinase, WW and PDZ domain containing 1 (Magi1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa49329	XM_008763133.2 Latest version!	Rattus norvegicus membrane associated guanylate kinase, WW and PDZ domain containing 1 (Magi1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa08314	NM_001030045.1 Latest version!	Rattus norvegicus membrane associated guanylate kinase, WW and PDZ domain containing 1 (Magi1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Magi1 ( XM_017592832.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448321.1 Rattus norvegicus membrane associated guanylate kinase, WW and PDZ domain containing 1 (Magi1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa60988
Clone ID Related Accession (Same CDS sequence)	XM_017592832.1
Accession Version	XM_017592832.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAG TGATCCAGAA GAAGAACCAC TGGACTGGCC GCGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GCATGGGGAG 
TTTCCGTACG TGGGGGCGGT GGCGGCGGCC GAGGCGGCGG GGCTTCCCGG CGGTGGCGAG 
GGACCGAAGC TGGCTGAAGG TGAGCTGCTG CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC 
TTGCCCCGCT ATGACGTGCT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA 
GCCGTCAGAC AAGGAGGAAG GCTCAACAAG GACCTGCGAC ATTTCCTCAA CCAACGGTTC 
CAGAAGGGGT CTCCGGATCA TGAACTCCAG CAGACCATAA GGGACAACCT CTACCGCCAT 
GCCGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAAG TGCCTGGCGT CGATTACAGC 
TTTCTGACTG TGAAGGAGTT CCTGGACCTC GAGCAGAGTG GGACCCTGTT GGAAGTCGGC 
ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCCA GCCAGCCAGT CAGTGGGAAA 
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CCAAGCAGTC GACCCCTAAG 
CGAACAAAAT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACACGGA GAATGAGGAA 
GAGGAGGATG TTCCTGAAAT GAACAGTAGC TTCACAGCCG ACTCTGGAGA CCAGGACGAG 
CCCACTCTCC AAGAAGCGAC GCTCCCGCCT GTGAATAGTA GCGCCCTTGC TGCTCCCATC 
ACGGACCCTT CTCAGAAGTT CCCTCAGTAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT 
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC 
CACAACACGA AAACAACATC ATGGTTAGAC CCTCGGTGCC TGAACAAACA GCAGAAGCCT 
CTGGAAGAAT GTGAAGATGA TGAAGAAGGG GTACACACCG AGGAGCTGGA CAGTGAACTA 
GAGCTGCCTG CTGGCTGGGA AAAGATTGAA GACCCTGTCT ACGGTGTCTA CTATGTAGAC 
CACATCAACA GGAAGACGCA ATATGAAAAC CCAGTCCTAG AAGCCAAAAG GAAGAGACAG 
CTTGAACAAC AGCAGCAGCA GCAGCAGCAC CAGCAGCAGC CGCAGCAGCC TCAGCCACCG 
CAGCCAGAAG AGTGGACAGA AGATCATGCA TCTGTTGTGC CTCCTGTTGC TCCTTCCCAT 
CCCCCAAGCA ACCCGGAGCC AGCCAGGGAA GCTCCACTTC AGGGCAAACC TTTTTTTACA 
AGAAACCCTT CTGAGCTGAA AGGCAAGTTC ATTCACACAA AGCTACGGAA AAGCAGTCGA 
GGCTTTGGCT TCACGGTGGT TGGAGGGGAC GAACCTGATG AGTTCCTGCA GATCAAGAGC 
CTTGTCCTAG ATGGTCCTGC TGCACTGGAT GGCAAGATGG AGACAGGAGA TGTAATCGTC 
AGTGTGAATG ACACCTGTGT TTTGGGACAC ACACATGCTC AAGTTGTGAA AATTTTCCAG 
TCCATTCCCA TTGGTGCCAG TGTGGACCTT GAACTCTGCA GAGGTTATCC ATTGCCTTTT 
GACCCGGATG ACCCTAACAC AAGTTTAGTG ACCTCGGTGG CCATTTTGGA CAAAGAACCA 
ATAATTGTAA ACGGACAAGA GACCTATGAT TCACCAGCAA GCCACAGTAG TAAAACGGGC 
AAAGTCAGCA ACATGAAGGA TGCCAGGCCA AGCAGCCCCG CAGACGTGGC TTCCAACAGT 
TCTCATGGGT ATCCCAACGA CACAGTCTCC TTGGCTTCCT CCATAGCCAC CCAGCCAGAG 
CTAATAACCG TTCACATAGT CAAAGGGCCA ATGGGATTTG GCTTTACGAT CGCAGATAGT 
CCCGGTGGGG GTGGCCAAAG AGTGAAGCAG ATTGTTGACA GTCCGCGCTG CAGAGGCCTC 
AAAGAAGGGG ATCTGATTGT GGAGGTGAAT AAGAGGAACG TGCAGGCCCT GACGCACAAT 
CAAGTCGTGG ATATGCTGAT TGAGTGTCCA AAGGGAAGTG AGGTCACACT GTTGGTGCAG 
CGAGGAGGGC TACCAGTTCC CAAGAAGAGT CCAAAGTCGC AGCCACTGGA GAGGAAAGAC 
AGCCAGAACA GCTCCCAGCA CAGTGTGTCC AGCCTCCGGA GCCTGCACAC AGCTTCCCCG 
AGCCACAGTG CACAGGTGCT CCCTGAGTAC CCACCTGCAG ACGTCCCTGC TCCAGATCAG 
ACCGACAGCT CTGGGCAGAA AAAGCCAGAT CCCTTTAAAA TCTGGGCCCA GTCGAGGAGC 
ATGTATGAAA ACCGACCTAT GTCACCTTCG CCTGCGTCAG GACTGAGCAA GGGTGAAAGA 
GACAGAGAGA TCAATTCCAC GAATTTTGGA GAATGTCAGA TTCCAGATTA CCAGGAGCAG 
GACATCTTCC TCTGGAGAAA AGAGACCGGT TTTGGATTTA GGATTCTGGG TGGAAATGAG 
CCAGGGGAAC CCATTTATAT CGGTCACATC GTACCACTGG GTGCTGCTGA CACAGACGGC 
CGCCTGAGGT CTGGAGATGA ATTAATCTGT GTGGATGGGA CACCAGTAAT TGGGAAATCA 
CACCAGCTCG TGGTCCAGCT TATGCAACAA GCTGCCAAGC AAGGCCACGT CAATCTCACA 
GTGAGGCGTA AAGTGGTGTT TACGGTCCCC AAAGCAGAGA ACGAGGTGCC CTCACCAGCC 
TCATCGCACC ACAGTAGCAA CCAGCCAGCG TCCCTGACGG AAGAGAAGCG CACACCGCAG 
GGCAGCCAGA ACTCGCTGAA CACCGTGAGC TCAGGCAGCG GCAGCACCAG CGGCATTGGC 
AGTGGTGGTG GCGGAGGCAG CGGCGTGGTG AGCGCTGTGC TGCAGCCATA TGATGTGGAG 
ATTCGGCGTG GGGAGAACGA GGGCTTTGGG TTTGTCATCG TGTCCTCCGT GAGCAGGCCT 
GAAGCGGGCA CCACCTTCGG TCGGATTATT GAGGGGAGCC CTGCTGACCG CTGTGGCAAG 
CTGAAAGTAG GAGACCGGAT CTTGGCGGTA AATGGATGTT CCATCACCAA CAAATCCCAT 
TCTGACATTG TCAACCTAAT CAAAGAAGCA GGCAACACAG TGACTCTCCG CATCATCCCC 
GGGGATGAGT CCTCCAATGC CACACTGCTG ACTAATGCGG AGAAGATTGC CACCATCACC 
ACCACTCATG CCCCCTCACA ACAGGGGACC CAGGAAACAA GGACCACCAC CAAACCAAAG 
CCGGATTCTC AGTTTGAGTT CAAAGGACCG CAGGCTACAC AGGAGCAAGA TTTTTACACT 
GTGGAATTGG AAAGAGGAGC CAAGGGATTT GGCTTTAGTC TTCGAGGAGG GCGAGAGTAT 
AACATGGACC TTTATGTTCT GCGCTTAGCA GAGGATGGTC CTGCAGAAAG ATGCGGAAAG 
ATGAGGATTG GCGATGAAAT TCTAGAGATC AATGGTGAGA CCACCAAAAA CATGAAACAC 
TCTCGGGCCA TAGAACTGAT CAAGAATGGC GGCCGCAGAG TCCGTCTGTT TCTGAGGCGG 
GGAGACGGCT CGGTCCCAGA ATATGACCCC AGCAGCGACA GGAACGGCCC CTCCACCGGT 
GCACAAGGTG TTCCGGAAGT GAGGCTGGGG CCACCGGACC ACAGATCACA TCCAGCCTTG 
GAGTCCAGTT ACCCACCCGA TCTTCACAAA TCATCACAAC ATGCCGAAAA GCGAGCACAC 
GCGAAGGATC CGAAAGGCAA CAGGGAGCAC AGCGGACAGC CCAACGAACA TCACACTTGG 
AACGGAACTT CTAGAAAACC CGACAGTGGG GCATGCCGGC CCAAAGACCG GCCACCCGAC 
ACATGGAGAG AGGCGCAGCC GGAATGGACA GCCACCAATG GCCCAAAGAG GAGGTCTCCG 
GAGAAGCGCA GGGAAGGCAC CCGCAGTGCT GACAACACTT TGGAAAGAAG GGAGAAGCAT 
GAGAAGAGAA GAGAGATGTC TCCTGAGAGG AAGCGAGAGC GGTCACCCAC CCGGAGAAAA 
GACAGCTCCC CGAGCCGCCG GCGGAGGTCC CTCGAAAGAC TCCTGGATCA AAGAAGGTCC 
CCAGAGCGCA GAAGAGGGGG CTCGCCCGAG AGGAGAGCAA AGTCCACCGA CAGGAGGAGG 
GCCAGGTCCC CAGAGCGCAG GCGAGAGCGG TCACTGGACA AAAGGAACCG AGACGACAAA 
GTTGGCCACC GAGAAAGGGA GGAGGCTGGT CTGAAGCTGG AAGCCGGGAG AAGCCCCAGA 
AATCCCCCAG AGCAGAGAAG GCGGCCTTAC AAAGAATGTA GCACCGACCT CAGCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448321.1
CDS	604..5043
Translation	MSKVIQKKNHWTGRVHECTVKRGPQGELGVTVLGGAEHGEFPYVGAVAAAEAAGLPGGGEGPKLAEGELLLEVQGVRVSGLPRYDVLGVIDSCKEAVTFKAVRQGGRLNKDLRHFLNQRFQKGSPDHELQQTIRDNLYRHAVPCTTRSPREGEVPGVDYSFLTVKEFLDLEQSGTLLEVGTYEGNYYGTPKPPSQPVSGKVITTDALHSLQSGSKQSTPKRTKSYNDMQNAGIVHTENEEEEDVPEMNSSFTADSGDQDEPTLQEATLPPVNSSALAAPITDPSQKFPQYLPLSAEDNLGPLPENWEMAYTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDEEGVHTEELDSELELPAGWEKIEDPVYGVYYVDHINRKTQYENPVLEAKRKRQLEQQQQQQQHQQQPQQPQPPQPEEWTEDHASVVPPVAPSHPPSNPEPAREAPLQGKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNDTCVLGHTHAQVVKIFQSIPIGASVDLELCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETYDSPASHSSKTGKVSNMKDARPSSPADVASNSSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKRNVQALTHNQVVDMLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSLRSLHTASPSHSAQVLPEYPPADVPAPDQTDSSGQKKPDPFKIWAQSRSMYENRPMSPSPASGLSKGERDREINSTNFGECQIPDYQEQDIFLWRKETGFGFRILGGNEPGEPIYIGHIVPLGAADTDGRLRSGDELICVDGTPVIGKSHQLVVQLMQQAAKQGHVNLTVRRKVVFTVPKAENEVPSPASSHHSSNQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGVVSAVLQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNATLLTNAEKIATITTTHAPSQQGTQETRTTTKPKPDSQFEFKGPQATQEQDFYTVELERGAKGFGFSLRGGREYNMDLYVLRLAEDGPAERCGKMRIGDEILEINGETTKNMKHSRAIELIKNGGRRVRLFLRRGDGSVPEYDPSSDRNGPSTGAQGVPEVRLGPPDHRSHPALESSYPPDLHKSSQHAEKRAHAKDPKGNREHSGQPNEHHTWNGTSRKPDSGACRPKDRPPDTWREAQPEWTATNGPKRRSPEKRREGTRSADNTLERREKHEKRREMSPERKRERSPTRRKDSSPSRRRRSLERLLDQRRSPERRRGGSPERRAKSTDRRRARSPERRRERSLDKRNRDDKVGHREREEAGLKLEAGRSPRNPPEQRRRPYKECSTDLSI

Target ORF information:

RefSeq Version	XM_017592832.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus membrane associated guanylate kinase, WW and PDZ domain containing 1 (Magi1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592832.1

ORF Insert Sequence:

ATGTCGAAAG TGATCCAGAA GAAGAACCAC TGGACTGGCC GCGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GCATGGGGAG 
TTTCCGTACG TGGGGGCGGT GGCGGCGGCC GAGGCGGCGG GGCTTCCCGG CGGTGGCGAG 
GGACCGAAGC TGGCTGAAGG TGAGCTGCTG CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC 
TTGCCCCGCT ATGACGTGCT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA 
GCCGTCAGAC AAGGAGGAAG GCTCAACAAG GACCTGCGAC ATTTCCTCAA CCAACGGTTC 
CAGAAGGGGT CTCCGGATCA TGAACTCCAG CAGACCATAA GGGACAACCT CTACCGCCAT 
GCCGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAAG TGCCTGGCGT CGATTACAGC 
TTTCTGACTG TGAAGGAGTT CCTGGACCTC GAGCAGAGTG GGACCCTGTT GGAAGTCGGC 
ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCCA GCCAGCCAGT CAGTGGGAAA 
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CCAAGCAGTC GACCCCTAAG 
CGAACAAAAT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACACGGA GAATGAGGAA 
GAGGAGGATG TTCCTGAAAT GAACAGTAGC TTCACAGCCG ACTCTGGAGA CCAGGACGAG 
CCCACTCTCC AAGAAGCGAC GCTCCCGCCT GTGAATAGTA GCGCCCTTGC TGCTCCCATC 
ACGGACCCTT CTCAGAAGTT CCCTCAGTAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT 
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC 
CACAACACGA AAACAACATC ATGGTTAGAC CCTCGGTGCC TGAACAAACA GCAGAAGCCT 
CTGGAAGAAT GTGAAGATGA TGAAGAAGGG GTACACACCG AGGAGCTGGA CAGTGAACTA 
GAGCTGCCTG CTGGCTGGGA AAAGATTGAA GACCCTGTCT ACGGTGTCTA CTATGTAGAC 
CACATCAACA GGAAGACGCA ATATGAAAAC CCAGTCCTAG AAGCCAAAAG GAAGAGACAG 
CTTGAACAAC AGCAGCAGCA GCAGCAGCAC CAGCAGCAGC CGCAGCAGCC TCAGCCACCG 
CAGCCAGAAG AGTGGACAGA AGATCATGCA TCTGTTGTGC CTCCTGTTGC TCCTTCCCAT 
CCCCCAAGCA ACCCGGAGCC AGCCAGGGAA GCTCCACTTC AGGGCAAACC TTTTTTTACA 
AGAAACCCTT CTGAGCTGAA AGGCAAGTTC ATTCACACAA AGCTACGGAA AAGCAGTCGA 
GGCTTTGGCT TCACGGTGGT TGGAGGGGAC GAACCTGATG AGTTCCTGCA GATCAAGAGC 
CTTGTCCTAG ATGGTCCTGC TGCACTGGAT GGCAAGATGG AGACAGGAGA TGTAATCGTC 
AGTGTGAATG ACACCTGTGT TTTGGGACAC ACACATGCTC AAGTTGTGAA AATTTTCCAG 
TCCATTCCCA TTGGTGCCAG TGTGGACCTT GAACTCTGCA GAGGTTATCC ATTGCCTTTT 
GACCCGGATG ACCCTAACAC AAGTTTAGTG ACCTCGGTGG CCATTTTGGA CAAAGAACCA 
ATAATTGTAA ACGGACAAGA GACCTATGAT TCACCAGCAA GCCACAGTAG TAAAACGGGC 
AAAGTCAGCA ACATGAAGGA TGCCAGGCCA AGCAGCCCCG CAGACGTGGC TTCCAACAGT 
TCTCATGGGT ATCCCAACGA CACAGTCTCC TTGGCTTCCT CCATAGCCAC CCAGCCAGAG 
CTAATAACCG TTCACATAGT CAAAGGGCCA ATGGGATTTG GCTTTACGAT CGCAGATAGT 
CCCGGTGGGG GTGGCCAAAG AGTGAAGCAG ATTGTTGACA GTCCGCGCTG CAGAGGCCTC 
AAAGAAGGGG ATCTGATTGT GGAGGTGAAT AAGAGGAACG TGCAGGCCCT GACGCACAAT 
CAAGTCGTGG ATATGCTGAT TGAGTGTCCA AAGGGAAGTG AGGTCACACT GTTGGTGCAG 
CGAGGAGGGC TACCAGTTCC CAAGAAGAGT CCAAAGTCGC AGCCACTGGA GAGGAAAGAC 
AGCCAGAACA GCTCCCAGCA CAGTGTGTCC AGCCTCCGGA GCCTGCACAC AGCTTCCCCG 
AGCCACAGTG CACAGGTGCT CCCTGAGTAC CCACCTGCAG ACGTCCCTGC TCCAGATCAG 
ACCGACAGCT CTGGGCAGAA AAAGCCAGAT CCCTTTAAAA TCTGGGCCCA GTCGAGGAGC 
ATGTATGAAA ACCGACCTAT GTCACCTTCG CCTGCGTCAG GACTGAGCAA GGGTGAAAGA 
GACAGAGAGA TCAATTCCAC GAATTTTGGA GAATGTCAGA TTCCAGATTA CCAGGAGCAG 
GACATCTTCC TCTGGAGAAA AGAGACCGGT TTTGGATTTA GGATTCTGGG TGGAAATGAG 
CCAGGGGAAC CCATTTATAT CGGTCACATC GTACCACTGG GTGCTGCTGA CACAGACGGC 
CGCCTGAGGT CTGGAGATGA ATTAATCTGT GTGGATGGGA CACCAGTAAT TGGGAAATCA 
CACCAGCTCG TGGTCCAGCT TATGCAACAA GCTGCCAAGC AAGGCCACGT CAATCTCACA 
GTGAGGCGTA AAGTGGTGTT TACGGTCCCC AAAGCAGAGA ACGAGGTGCC CTCACCAGCC 
TCATCGCACC ACAGTAGCAA CCAGCCAGCG TCCCTGACGG AAGAGAAGCG CACACCGCAG 
GGCAGCCAGA ACTCGCTGAA CACCGTGAGC TCAGGCAGCG GCAGCACCAG CGGCATTGGC 
AGTGGTGGTG GCGGAGGCAG CGGCGTGGTG AGCGCTGTGC TGCAGCCATA TGATGTGGAG 
ATTCGGCGTG GGGAGAACGA GGGCTTTGGG TTTGTCATCG TGTCCTCCGT GAGCAGGCCT 
GAAGCGGGCA CCACCTTCGG TCGGATTATT GAGGGGAGCC CTGCTGACCG CTGTGGCAAG 
CTGAAAGTAG GAGACCGGAT CTTGGCGGTA AATGGATGTT CCATCACCAA CAAATCCCAT 
TCTGACATTG TCAACCTAAT CAAAGAAGCA GGCAACACAG TGACTCTCCG CATCATCCCC 
GGGGATGAGT CCTCCAATGC CACACTGCTG ACTAATGCGG AGAAGATTGC CACCATCACC 
ACCACTCATG CCCCCTCACA ACAGGGGACC CAGGAAACAA GGACCACCAC CAAACCAAAG 
CCGGATTCTC AGTTTGAGTT CAAAGGACCG CAGGCTACAC AGGAGCAAGA TTTTTACACT 
GTGGAATTGG AAAGAGGAGC CAAGGGATTT GGCTTTAGTC TTCGAGGAGG GCGAGAGTAT 
AACATGGACC TTTATGTTCT GCGCTTAGCA GAGGATGGTC CTGCAGAAAG ATGCGGAAAG 
ATGAGGATTG GCGATGAAAT TCTAGAGATC AATGGTGAGA CCACCAAAAA CATGAAACAC 
TCTCGGGCCA TAGAACTGAT CAAGAATGGC GGCCGCAGAG TCCGTCTGTT TCTGAGGCGG 
GGAGACGGCT CGGTCCCAGA ATATGACCCC AGCAGCGACA GGAACGGCCC CTCCACCGGT 
GCACAAGGTG TTCCGGAAGT GAGGCTGGGG CCACCGGACC ACAGATCACA TCCAGCCTTG 
GAGTCCAGTT ACCCACCCGA TCTTCACAAA TCATCACAAC ATGCCGAAAA GCGAGCACAC 
GCGAAGGATC CGAAAGGCAA CAGGGAGCAC AGCGGACAGC CCAACGAACA TCACACTTGG 
AACGGAACTT CTAGAAAACC CGACAGTGGG GCATGCCGGC CCAAAGACCG GCCACCCGAC 
ACATGGAGAG AGGCGCAGCC GGAATGGACA GCCACCAATG GCCCAAAGAG GAGGTCTCCG 
GAGAAGCGCA GGGAAGGCAC CCGCAGTGCT GACAACACTT TGGAAAGAAG GGAGAAGCAT 
GAGAAGAGAA GAGAGATGTC TCCTGAGAGG AAGCGAGAGC GGTCACCCAC CCGGAGAAAA 
GACAGCTCCC CGAGCCGCCG GCGGAGGTCC CTCGAAAGAC TCCTGGATCA AAGAAGGTCC 
CCAGAGCGCA GAAGAGGGGG CTCGCCCGAG AGGAGAGCAA AGTCCACCGA CAGGAGGAGG 
GCCAGGTCCC CAGAGCGCAG GCGAGAGCGG TCACTGGACA AAAGGAACCG AGACGACAAA 
GTTGGCCACC GAGAAAGGGA GGAGGCTGGT CTGAAGCTGG AAGCCGGGAG AAGCCCCAGA 
AATCCCCCAG AGCAGAGAAG GCGGCCTTAC AAAGAATGTA GCACCGACCT CAGCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Magi1 ( XM_006236927.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236989.1 Rattus norvegicus membrane associated guanylate kinase, WW and PDZ domain containing 1 (Magi1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa27495
Clone ID Related Accession (Same CDS sequence)	XM_006236927.3
Accession Version	XM_006236927.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006236927.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAG TGATCCAGAA GAAGAACCAC TGGACTGGCC GCGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GCATGGGGAG 
TTTCCGTACG TGGGGGCGGT GGCGGCGGCC GAGGCGGCGG GGCTTCCCGG CGGTGGCGAG 
GGACCGAAGC TGGCTGAAGG TGAGCTGCTG CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC 
TTGCCCCGCT ATGACGTGCT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA 
GCCGTCAGAC AAGGAGGAAG GCTCAACAAG GACCTGCGAC ATTTCCTCAA CCAACGGTTC 
CAGAAGGGGT CTCCGGATCA TGAACTCCAG CAGACCATAA GGGACAACCT CTACCGCCAT 
GCCGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAAG TGCCTGGCGT CGATTACAGC 
TTTCTGACTG TGAAGGAGTT CCTGGACCTC GAGCAGAGTG GGACCCTGTT GGAAGTCGGC 
ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCCA GCCAGCCAGT CAGTGGGAAA 
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CCAAGCAGTC GACCCCTAAG 
CGAACAAAAT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACACGGA GAATGAGGAA 
GAGGAGGATG TTCCTGAAAT GAACAGTAGC TTCACAGCCG ACTCTGGAGA CCAGGACGAG 
CCCACTCTCC AAGAAGCGAC GCTCCCGCCT GTGAATAGTA GCGCCCTTGC TGCTCCCATC 
ACGGACCCTT CTCAGAAGTT CCCTCAGTAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT 
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC 
CACAACACGA AAACAACATC ATGGTTAGAC CCTCGGTGCC TGAACAAACA GCAGAAGCCT 
CTGGAAGAAT GTGAAGATGA TGAAGGGGTA CACACCGAGG AGCTGGACAG TGAACTAGAG 
CTGCCTGCTG GCTGGGAAAA GATTGAAGAC CCTGTCTACG GTGTCTACTA TGTAGACCAC 
ATCAACAGGA AGACGCAATA TGAAAACCCA GTCCTAGAAG CCAAAAGGAA GAGACAGCTT 
GAACAACAGC AGCAGCAGCA GCAGCACCAG CAGCAGCCGC AGCAGCCTCA GCCACCGCAG 
CCAGAAGAGT GGACAGAAGA TCATGCATCT GTTGTGCCTC CTGTTGCTCC TTCCCATCCC 
CCAAGCAACC CGGAGCCAGC CAGGGAAGCT CCACTTCAGG GCAAACCTTT TTTTACAAGA 
AACCCTTCTG AGCTGAAAGG CAAGTTCATT CACACAAAGC TACGGAAAAG CAGTCGAGGC 
TTTGGCTTCA CGGTGGTTGG AGGGGACGAA CCTGATGAGT TCCTGCAGAT CAAGAGCCTT 
GTCCTAGATG GTCCTGCTGC ACTGGATGGC AAGATGGAGA CAGGAGATGT AATCGTCAGT 
GTGAATGACA CCTGTGTTTT GGGACACACA CATGCTCAAG TTGTGAAAAT TTTCCAGTCC 
ATTCCCATTG GTGCCAGTGT GGACCTTGAA CTCTGCAGAG GTTATCCATT GCCTTTTGAC 
CCGGATGACC CTAACACAAG TTTAGTGACC TCGGTGGCCA TTTTGGACAA AGAACCAATA 
ATTGTAAACG GACAAGAGAC CTATGATTCA CCAGCAAGCC ACAGTAGTAA AACGGGCAAA 
GTCAGCAACA TGAAGGATGC CAGGCCAAGC AGCCCCGCAG ACGTGGCTTC CAACAGTTCT 
CATGGGTATC CCAACGACAC AGTCTCCTTG GCTTCCTCCA TAGCCACCCA GCCAGAGCTA 
ATAACCGTTC ACATAGTCAA AGGGCCAATG GGATTTGGCT TTACGATCGC AGATAGTCCC 
GGTGGGGGTG GCCAAAGAGT GAAGCAGATT GTTGACAGTC CGCGCTGCAG AGGCCTCAAA 
GAAGGGGATC TGATTGTGGA GGTGAATAAG AGGAACGTGC AGGCCCTGAC GCACAATCAA 
GTCGTGGATA TGCTGATTGA GTGTCCAAAG GGAAGTGAGG TCACACTGTT GGTGCAGCGA 
GGAGGGCTAC CAGTTCCCAA GAAGAGTCCA AAGTCGCAGC CACTGGAGAG GAAAGACAGC 
CAGAACAGCT CCCAGCACAG TGTGTCCAGC CTCCGGAGCC TGCACACAGC TTCCCCGAGC 
CACAGTGCAC AGGTGCTCCC TGAGTACCCA CCTGCAGACG TCCCTGCTCC AGATCAGACC 
GACAGCTCTG GGCAGAAAAA GCCAGATCCC TTTAAAATCT GGGCCCAGTC GAGGAGCATG 
TATGAAAACC GACCTATGTC ACCTTCGCCT GCGTCAGGAC TGAGCAAGGG TGAAAGAGAC 
AGAGAGATCA ATTCCACGAA TTTTGGAGAA TGTCAGATTC CAGATTACCA GGAGCAGGAC 
ATCTTCCTCT GGAGAAAAGA GACCGGTTTT GGATTTAGGA TTCTGGGTGG AAATGAGCCA 
GGGGAACCCA TTTATATCGG TCACATCGTA CCACTGGGTG CTGCTGACAC AGACGGCCGC 
CTGAGGTCTG GAGATGAATT AATCTGTGTG GATGGGACAC CAGTAATTGG GAAATCACAC 
CAGCTCGTGG TCCAGCTTAT GCAACAAGCT GCCAAGCAAG GCCACGTCAA TCTCACAGTG 
AGGCGTAAAG TGGTGTTTAC GGTCCCCAAA GCAGAGAACG AGGTGCCCTC ACCAGCCTCA 
TCGCACCACA GTAGCAACCA GCCAGCGTCC CTGACGGAAG AGAAGCGCAC ACCGCAGGGC 
AGCCAGAACT CGCTGAACAC CGTGAGCTCA GGCAGCGGCA GCACCAGCGG CATTGGCAGT 
GGTGGTGGCG GAGGCAGCGG CGTGGTGAGC GCTGTGCTGC AGCCATATGA TGTGGAGATT 
CGGCGTGGGG AGAACGAGGG CTTTGGGTTT GTCATCGTGT CCTCCGTGAG CAGGCCTGAA 
GCGGGCACCA CCTTCGCAGG CAATGCATGT GTGGCTATGC CTCACAAAAT AGGTCGGATT 
ATTGAGGGGA GCCCTGCTGA CCGCTGTGGC AAGCTGAAAG TAGGAGACCG GATCTTGGCG 
GTAAATGGAT GTTCCATCAC CAACAAATCC CATTCTGACA TTGTCAACCT AATCAAAGAA 
GCAGGCAACA CAGTGACTCT CCGCATCATC CCCGGGGATG AGTCCTCCAA TGCCACACTG 
CTGACTAATG CGGAGAAGAT TGCCACCATC ACCACCACTC ATGCCCCCTC ACAACAGGGG 
ACCCAGGAAA CAAGGACCAC CACCAAACCA AAGCCGGATT CTCAGTTTGA GTTCAAAGGA 
CCGCAGGCTA CACAGGAGCA AGATTTTTAC ACTGTGGAAT TGGAAAGAGG AGCCAAGGGA 
TTTGGCTTTA GTCTTCGAGG AGGGCGAGAG TATAACATGG ACCTTTATGT TCTGCGCTTA 
GCAGAGGATG GTCCTGCAGA AAGATGCGGA AAGATGAGGA TTGGCGATGA AATTCTAGAG 
ATCAATGGTG AGACCACCAA AAACATGAAA CACTCTCGGG CCATAGAACT GATCAAGAAT 
GGCGGCCGCA GAGTCCGTCT GTTTCTGAGG CGGGGAGACG GCTCGGTCCC AGAATATGAC 
CCCAGCAGCG ACAGGAACGG CCCCTCCACC GGTGCACAAG GTGTTCCGGA AGTGAGGCTG 
GGGCCACCGG ACCACAGATC ACATCCAGCC TTGGAGTCCA GTTACCCACC CGATCTTCAC 
AAATCATCAC AACATGCCGA AAAGCGAGCA CACGCGAAGG ATCCGAAAGG CAACAGGGAG 
CACAGCGGAC AGCCCAACGA ACATCACACT TGGAACGGAA CTTCTAGAAA ACCCGACAGT 
GGGGCATGCC GGCCCAAAGA CCGGCCACCC GACACATGGA GAGAGGCGCA GCCGGAATGG 
ACAGCCACCA ATGGCCCAAA GAGGAGGTCT CCGGAGAAGC GCAGGGAAGG CACCCGCAGT 
GCTGACAACA CTTTGGAAAG AAGGGAGAAG CATGAGAAGA GAAGAGAGAT GTCTCCTGAG 
AGGAAGCGAG AGCGGTCACC CACCCGGAGA AAAGACAGCT CCCCGAGCCG CCGGCGGAGG 
TCCCTCGAAA GACTCCTGGA TCAAAGAAGG TCCCCAGAGC GCAGAAGAGG GGGCTCGCCC 
GAGAGGAGAG CAAAGTCCAC CGACAGGAGG AGGGCCAGGT CCCCAGAGCG CAGGCGAGAG 
CGGTCACTGG ACAAAAGGAA CCGAGACGAC AAAGTTGGCC ACCGAGAAAG GGAGGAGGCT 
GGTCTGAAGC TGGAAGCCGG GAGAAGCCCC AGAAATCCCC CAGAGCAGAG AAGGCGGCCT 
TACAAAGAAT GTAGCACCGA CCTCAGCATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236989.1
CDS	604..5076
Translation	MSKVIQKKNHWTGRVHECTVKRGPQGELGVTVLGGAEHGEFPYVGAVAAAEAAGLPGGGEGPKLAEGELLLEVQGVRVSGLPRYDVLGVIDSCKEAVTFKAVRQGGRLNKDLRHFLNQRFQKGSPDHELQQTIRDNLYRHAVPCTTRSPREGEVPGVDYSFLTVKEFLDLEQSGTLLEVGTYEGNYYGTPKPPSQPVSGKVITTDALHSLQSGSKQSTPKRTKSYNDMQNAGIVHTENEEEEDVPEMNSSFTADSGDQDEPTLQEATLPPVNSSALAAPITDPSQKFPQYLPLSAEDNLGPLPENWEMAYTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDEGVHTEELDSELELPAGWEKIEDPVYGVYYVDHINRKTQYENPVLEAKRKRQLEQQQQQQQHQQQPQQPQPPQPEEWTEDHASVVPPVAPSHPPSNPEPAREAPLQGKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNDTCVLGHTHAQVVKIFQSIPIGASVDLELCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETYDSPASHSSKTGKVSNMKDARPSSPADVASNSSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKRNVQALTHNQVVDMLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSLRSLHTASPSHSAQVLPEYPPADVPAPDQTDSSGQKKPDPFKIWAQSRSMYENRPMSPSPASGLSKGERDREINSTNFGECQIPDYQEQDIFLWRKETGFGFRILGGNEPGEPIYIGHIVPLGAADTDGRLRSGDELICVDGTPVIGKSHQLVVQLMQQAAKQGHVNLTVRRKVVFTVPKAENEVPSPASSHHSSNQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGVVSAVLQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNATLLTNAEKIATITTTHAPSQQGTQETRTTTKPKPDSQFEFKGPQATQEQDFYTVELERGAKGFGFSLRGGREYNMDLYVLRLAEDGPAERCGKMRIGDEILEINGETTKNMKHSRAIELIKNGGRRVRLFLRRGDGSVPEYDPSSDRNGPSTGAQGVPEVRLGPPDHRSHPALESSYPPDLHKSSQHAEKRAHAKDPKGNREHSGQPNEHHTWNGTSRKPDSGACRPKDRPPDTWREAQPEWTATNGPKRRSPEKRREGTRSADNTLERREKHEKRREMSPERKRERSPTRRKDSSPSRRRRSLERLLDQRRSPERRRGGSPERRAKSTDRRRARSPERRRERSLDKRNRDDKVGHREREEAGLKLEAGRSPRNPPEQRRRPYKECSTDLSI

Target ORF information:

RefSeq Version	XM_006236927.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus membrane associated guanylate kinase, WW and PDZ domain containing 1 (Magi1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236927.3

ORF Insert Sequence:

ATGTCGAAAG TGATCCAGAA GAAGAACCAC TGGACTGGCC GCGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GCATGGGGAG 
TTTCCGTACG TGGGGGCGGT GGCGGCGGCC GAGGCGGCGG GGCTTCCCGG CGGTGGCGAG 
GGACCGAAGC TGGCTGAAGG TGAGCTGCTG CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC 
TTGCCCCGCT ATGACGTGCT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA 
GCCGTCAGAC AAGGAGGAAG GCTCAACAAG GACCTGCGAC ATTTCCTCAA CCAACGGTTC 
CAGAAGGGGT CTCCGGATCA TGAACTCCAG CAGACCATAA GGGACAACCT CTACCGCCAT 
GCCGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAAG TGCCTGGCGT CGATTACAGC 
TTTCTGACTG TGAAGGAGTT CCTGGACCTC GAGCAGAGTG GGACCCTGTT GGAAGTCGGC 
ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCCA GCCAGCCAGT CAGTGGGAAA 
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CCAAGCAGTC GACCCCTAAG 
CGAACAAAAT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACACGGA GAATGAGGAA 
GAGGAGGATG TTCCTGAAAT GAACAGTAGC TTCACAGCCG ACTCTGGAGA CCAGGACGAG 
CCCACTCTCC AAGAAGCGAC GCTCCCGCCT GTGAATAGTA GCGCCCTTGC TGCTCCCATC 
ACGGACCCTT CTCAGAAGTT CCCTCAGTAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT 
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC 
CACAACACGA AAACAACATC ATGGTTAGAC CCTCGGTGCC TGAACAAACA GCAGAAGCCT 
CTGGAAGAAT GTGAAGATGA TGAAGGGGTA CACACCGAGG AGCTGGACAG TGAACTAGAG 
CTGCCTGCTG GCTGGGAAAA GATTGAAGAC CCTGTCTACG GTGTCTACTA TGTAGACCAC 
ATCAACAGGA AGACGCAATA TGAAAACCCA GTCCTAGAAG CCAAAAGGAA GAGACAGCTT 
GAACAACAGC AGCAGCAGCA GCAGCACCAG CAGCAGCCGC AGCAGCCTCA GCCACCGCAG 
CCAGAAGAGT GGACAGAAGA TCATGCATCT GTTGTGCCTC CTGTTGCTCC TTCCCATCCC 
CCAAGCAACC CGGAGCCAGC CAGGGAAGCT CCACTTCAGG GCAAACCTTT TTTTACAAGA 
AACCCTTCTG AGCTGAAAGG CAAGTTCATT CACACAAAGC TACGGAAAAG CAGTCGAGGC 
TTTGGCTTCA CGGTGGTTGG AGGGGACGAA CCTGATGAGT TCCTGCAGAT CAAGAGCCTT 
GTCCTAGATG GTCCTGCTGC ACTGGATGGC AAGATGGAGA CAGGAGATGT AATCGTCAGT 
GTGAATGACA CCTGTGTTTT GGGACACACA CATGCTCAAG TTGTGAAAAT TTTCCAGTCC 
ATTCCCATTG GTGCCAGTGT GGACCTTGAA CTCTGCAGAG GTTATCCATT GCCTTTTGAC 
CCGGATGACC CTAACACAAG TTTAGTGACC TCGGTGGCCA TTTTGGACAA AGAACCAATA 
ATTGTAAACG GACAAGAGAC CTATGATTCA CCAGCAAGCC ACAGTAGTAA AACGGGCAAA 
GTCAGCAACA TGAAGGATGC CAGGCCAAGC AGCCCCGCAG ACGTGGCTTC CAACAGTTCT 
CATGGGTATC CCAACGACAC AGTCTCCTTG GCTTCCTCCA TAGCCACCCA GCCAGAGCTA 
ATAACCGTTC ACATAGTCAA AGGGCCAATG GGATTTGGCT TTACGATCGC AGATAGTCCC 
GGTGGGGGTG GCCAAAGAGT GAAGCAGATT GTTGACAGTC CGCGCTGCAG AGGCCTCAAA 
GAAGGGGATC TGATTGTGGA GGTGAATAAG AGGAACGTGC AGGCCCTGAC GCACAATCAA 
GTCGTGGATA TGCTGATTGA GTGTCCAAAG GGAAGTGAGG TCACACTGTT GGTGCAGCGA 
GGAGGGCTAC CAGTTCCCAA GAAGAGTCCA AAGTCGCAGC CACTGGAGAG GAAAGACAGC 
CAGAACAGCT CCCAGCACAG TGTGTCCAGC CTCCGGAGCC TGCACACAGC TTCCCCGAGC 
CACAGTGCAC AGGTGCTCCC TGAGTACCCA CCTGCAGACG TCCCTGCTCC AGATCAGACC 
GACAGCTCTG GGCAGAAAAA GCCAGATCCC TTTAAAATCT GGGCCCAGTC GAGGAGCATG 
TATGAAAACC GACCTATGTC ACCTTCGCCT GCGTCAGGAC TGAGCAAGGG TGAAAGAGAC 
AGAGAGATCA ATTCCACGAA TTTTGGAGAA TGTCAGATTC CAGATTACCA GGAGCAGGAC 
ATCTTCCTCT GGAGAAAAGA GACCGGTTTT GGATTTAGGA TTCTGGGTGG AAATGAGCCA 
GGGGAACCCA TTTATATCGG TCACATCGTA CCACTGGGTG CTGCTGACAC AGACGGCCGC 
CTGAGGTCTG GAGATGAATT AATCTGTGTG GATGGGACAC CAGTAATTGG GAAATCACAC 
CAGCTCGTGG TCCAGCTTAT GCAACAAGCT GCCAAGCAAG GCCACGTCAA TCTCACAGTG 
AGGCGTAAAG TGGTGTTTAC GGTCCCCAAA GCAGAGAACG AGGTGCCCTC ACCAGCCTCA 
TCGCACCACA GTAGCAACCA GCCAGCGTCC CTGACGGAAG AGAAGCGCAC ACCGCAGGGC 
AGCCAGAACT CGCTGAACAC CGTGAGCTCA GGCAGCGGCA GCACCAGCGG CATTGGCAGT 
GGTGGTGGCG GAGGCAGCGG CGTGGTGAGC GCTGTGCTGC AGCCATATGA TGTGGAGATT 
CGGCGTGGGG AGAACGAGGG CTTTGGGTTT GTCATCGTGT CCTCCGTGAG CAGGCCTGAA 
GCGGGCACCA CCTTCGCAGG CAATGCATGT GTGGCTATGC CTCACAAAAT AGGTCGGATT 
ATTGAGGGGA GCCCTGCTGA CCGCTGTGGC AAGCTGAAAG TAGGAGACCG GATCTTGGCG 
GTAAATGGAT GTTCCATCAC CAACAAATCC CATTCTGACA TTGTCAACCT AATCAAAGAA 
GCAGGCAACA CAGTGACTCT CCGCATCATC CCCGGGGATG AGTCCTCCAA TGCCACACTG 
CTGACTAATG CGGAGAAGAT TGCCACCATC ACCACCACTC ATGCCCCCTC ACAACAGGGG 
ACCCAGGAAA CAAGGACCAC CACCAAACCA AAGCCGGATT CTCAGTTTGA GTTCAAAGGA 
CCGCAGGCTA CACAGGAGCA AGATTTTTAC ACTGTGGAAT TGGAAAGAGG AGCCAAGGGA 
TTTGGCTTTA GTCTTCGAGG AGGGCGAGAG TATAACATGG ACCTTTATGT TCTGCGCTTA 
GCAGAGGATG GTCCTGCAGA AAGATGCGGA AAGATGAGGA TTGGCGATGA AATTCTAGAG 
ATCAATGGTG AGACCACCAA AAACATGAAA CACTCTCGGG CCATAGAACT GATCAAGAAT 
GGCGGCCGCA GAGTCCGTCT GTTTCTGAGG CGGGGAGACG GCTCGGTCCC AGAATATGAC 
CCCAGCAGCG ACAGGAACGG CCCCTCCACC GGTGCACAAG GTGTTCCGGA AGTGAGGCTG 
GGGCCACCGG ACCACAGATC ACATCCAGCC TTGGAGTCCA GTTACCCACC CGATCTTCAC 
AAATCATCAC AACATGCCGA AAAGCGAGCA CACGCGAAGG ATCCGAAAGG CAACAGGGAG 
CACAGCGGAC AGCCCAACGA ACATCACACT TGGAACGGAA CTTCTAGAAA ACCCGACAGT 
GGGGCATGCC GGCCCAAAGA CCGGCCACCC GACACATGGA GAGAGGCGCA GCCGGAATGG 
ACAGCCACCA ATGGCCCAAA GAGGAGGTCT CCGGAGAAGC GCAGGGAAGG CACCCGCAGT 
GCTGACAACA CTTTGGAAAG AAGGGAGAAG CATGAGAAGA GAAGAGAGAT GTCTCCTGAG 
AGGAAGCGAG AGCGGTCACC CACCCGGAGA AAAGACAGCT CCCCGAGCCG CCGGCGGAGG 
TCCCTCGAAA GACTCCTGGA TCAAAGAAGG TCCCCAGAGC GCAGAAGAGG GGGCTCGCCC 
GAGAGGAGAG CAAAGTCCAC CGACAGGAGG AGGGCCAGGT CCCCAGAGCG CAGGCGAGAG 
CGGTCACTGG ACAAAAGGAA CCGAGACGAC AAAGTTGGCC ACCGAGAAAG GGAGGAGGCT 
GGTCTGAAGC TGGAAGCCGG GAGAAGCCCC AGAAATCCCC CAGAGCAGAG AAGGCGGCCT 
TACAAAGAAT GTAGCACCGA CCTCAGCATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Magi1 ( XM_006236928.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236990.1 Rattus norvegicus membrane associated guanylate kinase, WW and PDZ domain containing 1 (Magi1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa27496
Clone ID Related Accession (Same CDS sequence)	XM_006236928.3
Accession Version	XM_006236928.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006236928.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAG TGATCCAGAA GAAGAACCAC TGGACTGGCC GCGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GCATGGGGAG 
TTTCCGTACG TGGGGGCGGT GGCGGCGGCC GAGGCGGCGG GGCTTCCCGG CGGTGGCGAG 
GGACCGAAGC TGGCTGAAGG TGAGCTGCTG CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC 
TTGCCCCGCT ATGACGTGCT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA 
GCCGTCAGAC AAGGAGGAAG GCTCAACAAG GACCTGCGAC ATTTCCTCAA CCAACGGTTC 
CAGAAGGGGT CTCCGGATCA TGAACTCCAG CAGACCATAA GGGACAACCT CTACCGCCAT 
GCCGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAAG TGCCTGGCGT CGATTACAGC 
TTTCTGACTG TGAAGGAGTT CCTGGACCTC GAGCAGAGTG GGACCCTGTT GGAAGTCGGC 
ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCCA GCCAGCCAGT CAGTGGGAAA 
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CCAAGCAGTC GACCCCTAAG 
CGAACAAAAT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACACGGA GAATGAGGAA 
GAGGAGGATG TTCCTGAAAT GAACAGTAGC TTCACAGCCG ACTCTGGAGA CCAGGACGAG 
CCCACTCTCC AAGAAGCGAC GCTCCCGCCT GTGAATAGTA GCGCCCTTGC TGCTCCCATC 
ACGGACCCTT CTCAGAAGTT CCCTCAGTAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT 
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC 
CACAACACGA AAACAACATC ATGGTTAGAC CCTCGGTGCC TGAACAAACA GCAGAAGCCT 
CTGGAAGAAT GTGAAGATGA TGAAGGGGTA CACACCGAGG AGCTGGACAG TGAACTAGAG 
CTGCCTGCTG GCTGGGAAAA GATTGAAGAC CCTGTCTACG GTGTCTACTA TGTAGACCAC 
ATCAACAGGA AGACGCAATA TGAAAACCCA GTCCTAGAAG CCAAAAGGAA GAGACAGCTT 
GAACAACAGC AGCAGCAGCA GCAGCACCAG CAGCAGCCGC AGCAGCCTCA GCCACCGCAG 
CCAGAAGAGT GGACAGAAGA TCATGCATCT GTTGTGCCTC CTGTTGCTCC TTCCCATCCC 
CCAAGCAACC CGGAGCCAGC CAGGGAAGCT CCACTTCAGG GCAAACCTTT TTTTACAAGA 
AACCCTTCTG AGCTGAAAGG CAAGTTCATT CACACAAAGC TACGGAAAAG CAGTCGAGGC 
TTTGGCTTCA CGGTGGTTGG AGGGGACGAA CCTGATGAGT TCCTGCAGAT CAAGAGCCTT 
GTCCTAGATG GTCCTGCTGC ACTGGATGGC AAGATGGAGA CAGGAGATGT AATCGTCAGT 
GTGAATGACA CCTGTGTTTT GGGACACACA CATGCTCAAG TTGTGAAAAT TTTCCAGTCC 
ATTCCCATTG GTGCCAGTGT GGACCTTGAA CTCTGCAGAG GTTATCCATT GCCTTTTGAC 
CCGGATGACC CTAACACAAG TTTAGTGACC TCGGTGGCCA TTTTGGACAA AGAACCAATA 
ATTGTAAACG GACAAGAGAC CTATGATTCA CCAGCAAGCC ACAGTAGTAA AACGGGCAAA 
GTCAGCAACA TGAAGGATGC CAGGCCAAGC AGCCCCGCAG ACGTGGCTTC CAACAGTTCT 
CATGGGTATC CCAACGACAC AGTCTCCTTG GCTTCCTCCA TAGCCACCCA GCCAGAGCTA 
ATAACCGTTC ACATAGTCAA AGGGCCAATG GGATTTGGCT TTACGATCGC AGATAGTCCC 
GGTGGGGGTG GCCAAAGAGT GAAGCAGATT GTTGACAGTC CGCGCTGCAG AGGCCTCAAA 
GAAGGGGATC TGATTGTGGA GGTGAATAAG AGGAACGTGC AGGCCCTGAC GCACAATCAA 
GTCGTGGATA TGCTGATTGA GTGTCCAAAG GGAAGTGAGG TCACACTGTT GGTGCAGCGA 
GGAGGGCTAC CAGTTCCCAA GAAGAGTCCA AAGTCGCAGC CACTGGAGAG GAAAGACAGC 
CAGAACAGCT CCCAGCACAG TGTGTCCAGC CTCCGGAGCC TGCACACAGC TTCCCCGAGC 
CACAGTGCAC AGGTGCTCCC TGAGTACCCA CCTGCAGACG TCCCTGCTCC AGATCAGACC 
GACAGCTCTG GGCAGAAAAA GCCAGATCCC TTTAAAATCT GGGCCCAGTC GAGGAGCATG 
TATGAAAACC GACCTATGTC ACCTTCGCCT GCGTCAGGAC TGAGCAAGGG TGAAAGAGAC 
AGAGAGATCA ATTCCACGAA TTTTGGAGAA TGTCAGATTC CAGATTACCA GGAGCAGGAC 
ATCTTCCTCT GGAGAAAAGA GACCGGTTTT GGATTTAGGA TTCTGGGTGG AAATGAGCCA 
GGGGAACCCA TTTATATCGG TCACATCGTA CCACTGGGTG CTGCTGACAC AGACGGCCGC 
CTGAGGTCTG GAGATGAATT AATCTGTGTG GATGGGACAC CAGTAATTGG GAAATCACAC 
CAGCTCGTGG TCCAGCTTAT GCAACAAGCT GCCAAGCAAG GCCACGTCAA TCTCACAGTG 
AGGCGTAAAG TGGTGTTTAC GGTCCCCAAA GCAGAGAACG AGGTGCCCTC ACCAGCCTCA 
TCGCACCACA GTAGCAACCA GCCAGCGTCC CTGACGGAAG AGAAGCGCAC ACCGCAGGGC 
AGCCAGAACT CGCTGAACAC CGTGAGCTCA GGCAGCGGCA GCACCAGCGG CATTGGCAGT 
GGTGGTGGCG GAGGCAGCGG CGTGGTGAGC GCTGTGCTGC AGCCATATGA TGTGGAGATT 
CGGCGTGGGG AGAACGAGGG CTTTGGGTTT GTCATCGTGT CCTCCGTGAG CAGGCCTGAA 
GCGGGCACCA CCTTCGGTCG GATTATTGAG GGGAGCCCTG CTGACCGCTG TGGCAAGCTG 
AAAGTAGGAG ACCGGATCTT GGCGGTAAAT GGATGTTCCA TCACCAACAA ATCCCATTCT 
GACATTGTCA ACCTAATCAA AGAAGCAGGC AACACAGTGA CTCTCCGCAT CATCCCCGGG 
GATGAGTCCT CCAATGCCAC ACTGCTGACT AATGCGGAGA AGATTGCCAC CATCACCACC 
ACTCATGCCC CCTCACAACA GGGGACCCAG GAAACAAGGA CCACCACCAA ACCAAAGCCG 
GATTCTCAGT TTGAGTTCAA AGGACCGCAG GCTACACAGG AGCAAGATTT TTACACTGTG 
GAATTGGAAA GAGGAGCCAA GGGATTTGGC TTTAGTCTTC GAGGAGGGCG AGAGTATAAC 
ATGGACCTTT ATGTTCTGCG CTTAGCAGAG GATGGTCCTG CAGAAAGATG CGGAAAGATG 
AGGATTGGCG ATGAAATTCT AGAGATCAAT GGTGAGACCA CCAAAAACAT GAAACACTCT 
CGGGCCATAG AACTGATCAA GAATGGCGGC CGCAGAGTCC GTCTGTTTCT GAGGCGGGGA 
GACGGCTCGG TCCCAGAATA TGACCCCAGC AGCGACAGGA ACGGCCCCTC CACCGGTGCA 
CAAGGTGTTC CGGAAGTGAG GCTGGGGCCA CCGGACCACA GATCACATCC AGCCTTGGAG 
TCCAGTTACC CACCCGATCT TCACAAATCA TCACAACATG CCGAAAAGCG AGCACACGCG 
AAGGATCCGA AAGGCAACAG GGAGCACAGC GGACAGCCCA ACGAACATCA CACTTGGAAC 
GGAACTTCTA GAAAACCCGA CAGTGGGGCA TGCCGGCCCA AAGACCGGCC ACCCGACACA 
TGGAGAGAGG CGCAGCCGGA ATGGACAGCC ACCAATGGCC CAAAGAGGAG GTCTCCGGAG 
AAGCGCAGGG AAGGCACCCG CAGTGCTGAC AACACTTTGG AAAGAAGGGA GAAGCATGAG 
AAGAGAAGAG AGATGTCTCC TGAGAGGAAG CGAGAGCGGT CACCCACCCG GAGAAAAGAC 
AGCTCCCCGA GCCGCCGGCG GAGGTCCCTC GAAAGACTCC TGGATCAAAG AAGGTCCCCA 
GAGCGCAGAA GAGGGGGCTC GCCCGAGAGG AGAGCAAAGT CCACCGACAG GAGGAGGGCC 
AGGTCCCCAG AGCGCAGGCG AGAGCGGTCA CTGGACAAAA GGAACCGAGA CGACAAAGTT 
GGCCACCGAG AAAGGGAGGA GGCTGGTCTG AAGCTGGAAG CCGGGAGAAG CCCCAGAAAT 
CCCCCAGAGC AGAGAAGGCG GCCTTACAAA GAATGTAGCA CCGACCTCAG CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236990.1
CDS	604..5040
Translation	MSKVIQKKNHWTGRVHECTVKRGPQGELGVTVLGGAEHGEFPYVGAVAAAEAAGLPGGGEGPKLAEGELLLEVQGVRVSGLPRYDVLGVIDSCKEAVTFKAVRQGGRLNKDLRHFLNQRFQKGSPDHELQQTIRDNLYRHAVPCTTRSPREGEVPGVDYSFLTVKEFLDLEQSGTLLEVGTYEGNYYGTPKPPSQPVSGKVITTDALHSLQSGSKQSTPKRTKSYNDMQNAGIVHTENEEEEDVPEMNSSFTADSGDQDEPTLQEATLPPVNSSALAAPITDPSQKFPQYLPLSAEDNLGPLPENWEMAYTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDEGVHTEELDSELELPAGWEKIEDPVYGVYYVDHINRKTQYENPVLEAKRKRQLEQQQQQQQHQQQPQQPQPPQPEEWTEDHASVVPPVAPSHPPSNPEPAREAPLQGKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNDTCVLGHTHAQVVKIFQSIPIGASVDLELCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETYDSPASHSSKTGKVSNMKDARPSSPADVASNSSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKRNVQALTHNQVVDMLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSLRSLHTASPSHSAQVLPEYPPADVPAPDQTDSSGQKKPDPFKIWAQSRSMYENRPMSPSPASGLSKGERDREINSTNFGECQIPDYQEQDIFLWRKETGFGFRILGGNEPGEPIYIGHIVPLGAADTDGRLRSGDELICVDGTPVIGKSHQLVVQLMQQAAKQGHVNLTVRRKVVFTVPKAENEVPSPASSHHSSNQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGVVSAVLQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNATLLTNAEKIATITTTHAPSQQGTQETRTTTKPKPDSQFEFKGPQATQEQDFYTVELERGAKGFGFSLRGGREYNMDLYVLRLAEDGPAERCGKMRIGDEILEINGETTKNMKHSRAIELIKNGGRRVRLFLRRGDGSVPEYDPSSDRNGPSTGAQGVPEVRLGPPDHRSHPALESSYPPDLHKSSQHAEKRAHAKDPKGNREHSGQPNEHHTWNGTSRKPDSGACRPKDRPPDTWREAQPEWTATNGPKRRSPEKRREGTRSADNTLERREKHEKRREMSPERKRERSPTRRKDSSPSRRRRSLERLLDQRRSPERRRGGSPERRAKSTDRRRARSPERRRERSLDKRNRDDKVGHREREEAGLKLEAGRSPRNPPEQRRRPYKECSTDLSI

Target ORF information:

RefSeq Version	XM_006236928.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus membrane associated guanylate kinase, WW and PDZ domain containing 1 (Magi1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236928.3

ORF Insert Sequence:

ATGTCGAAAG TGATCCAGAA GAAGAACCAC TGGACTGGCC GCGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GCATGGGGAG 
TTTCCGTACG TGGGGGCGGT GGCGGCGGCC GAGGCGGCGG GGCTTCCCGG CGGTGGCGAG 
GGACCGAAGC TGGCTGAAGG TGAGCTGCTG CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC 
TTGCCCCGCT ATGACGTGCT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA 
GCCGTCAGAC AAGGAGGAAG GCTCAACAAG GACCTGCGAC ATTTCCTCAA CCAACGGTTC 
CAGAAGGGGT CTCCGGATCA TGAACTCCAG CAGACCATAA GGGACAACCT CTACCGCCAT 
GCCGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAAG TGCCTGGCGT CGATTACAGC 
TTTCTGACTG TGAAGGAGTT CCTGGACCTC GAGCAGAGTG GGACCCTGTT GGAAGTCGGC 
ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCCA GCCAGCCAGT CAGTGGGAAA 
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CCAAGCAGTC GACCCCTAAG 
CGAACAAAAT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACACGGA GAATGAGGAA 
GAGGAGGATG TTCCTGAAAT GAACAGTAGC TTCACAGCCG ACTCTGGAGA CCAGGACGAG 
CCCACTCTCC AAGAAGCGAC GCTCCCGCCT GTGAATAGTA GCGCCCTTGC TGCTCCCATC 
ACGGACCCTT CTCAGAAGTT CCCTCAGTAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT 
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC 
CACAACACGA AAACAACATC ATGGTTAGAC CCTCGGTGCC TGAACAAACA GCAGAAGCCT 
CTGGAAGAAT GTGAAGATGA TGAAGGGGTA CACACCGAGG AGCTGGACAG TGAACTAGAG 
CTGCCTGCTG GCTGGGAAAA GATTGAAGAC CCTGTCTACG GTGTCTACTA TGTAGACCAC 
ATCAACAGGA AGACGCAATA TGAAAACCCA GTCCTAGAAG CCAAAAGGAA GAGACAGCTT 
GAACAACAGC AGCAGCAGCA GCAGCACCAG CAGCAGCCGC AGCAGCCTCA GCCACCGCAG 
CCAGAAGAGT GGACAGAAGA TCATGCATCT GTTGTGCCTC CTGTTGCTCC TTCCCATCCC 
CCAAGCAACC CGGAGCCAGC CAGGGAAGCT CCACTTCAGG GCAAACCTTT TTTTACAAGA 
AACCCTTCTG AGCTGAAAGG CAAGTTCATT CACACAAAGC TACGGAAAAG CAGTCGAGGC 
TTTGGCTTCA CGGTGGTTGG AGGGGACGAA CCTGATGAGT TCCTGCAGAT CAAGAGCCTT 
GTCCTAGATG GTCCTGCTGC ACTGGATGGC AAGATGGAGA CAGGAGATGT AATCGTCAGT 
GTGAATGACA CCTGTGTTTT GGGACACACA CATGCTCAAG TTGTGAAAAT TTTCCAGTCC 
ATTCCCATTG GTGCCAGTGT GGACCTTGAA CTCTGCAGAG GTTATCCATT GCCTTTTGAC 
CCGGATGACC CTAACACAAG TTTAGTGACC TCGGTGGCCA TTTTGGACAA AGAACCAATA 
ATTGTAAACG GACAAGAGAC CTATGATTCA CCAGCAAGCC ACAGTAGTAA AACGGGCAAA 
GTCAGCAACA TGAAGGATGC CAGGCCAAGC AGCCCCGCAG ACGTGGCTTC CAACAGTTCT 
CATGGGTATC CCAACGACAC AGTCTCCTTG GCTTCCTCCA TAGCCACCCA GCCAGAGCTA 
ATAACCGTTC ACATAGTCAA AGGGCCAATG GGATTTGGCT TTACGATCGC AGATAGTCCC 
GGTGGGGGTG GCCAAAGAGT GAAGCAGATT GTTGACAGTC CGCGCTGCAG AGGCCTCAAA 
GAAGGGGATC TGATTGTGGA GGTGAATAAG AGGAACGTGC AGGCCCTGAC GCACAATCAA 
GTCGTGGATA TGCTGATTGA GTGTCCAAAG GGAAGTGAGG TCACACTGTT GGTGCAGCGA 
GGAGGGCTAC CAGTTCCCAA GAAGAGTCCA AAGTCGCAGC CACTGGAGAG GAAAGACAGC 
CAGAACAGCT CCCAGCACAG TGTGTCCAGC CTCCGGAGCC TGCACACAGC TTCCCCGAGC 
CACAGTGCAC AGGTGCTCCC TGAGTACCCA CCTGCAGACG TCCCTGCTCC AGATCAGACC 
GACAGCTCTG GGCAGAAAAA GCCAGATCCC TTTAAAATCT GGGCCCAGTC GAGGAGCATG 
TATGAAAACC GACCTATGTC ACCTTCGCCT GCGTCAGGAC TGAGCAAGGG TGAAAGAGAC 
AGAGAGATCA ATTCCACGAA TTTTGGAGAA TGTCAGATTC CAGATTACCA GGAGCAGGAC 
ATCTTCCTCT GGAGAAAAGA GACCGGTTTT GGATTTAGGA TTCTGGGTGG AAATGAGCCA 
GGGGAACCCA TTTATATCGG TCACATCGTA CCACTGGGTG CTGCTGACAC AGACGGCCGC 
CTGAGGTCTG GAGATGAATT AATCTGTGTG GATGGGACAC CAGTAATTGG GAAATCACAC 
CAGCTCGTGG TCCAGCTTAT GCAACAAGCT GCCAAGCAAG GCCACGTCAA TCTCACAGTG 
AGGCGTAAAG TGGTGTTTAC GGTCCCCAAA GCAGAGAACG AGGTGCCCTC ACCAGCCTCA 
TCGCACCACA GTAGCAACCA GCCAGCGTCC CTGACGGAAG AGAAGCGCAC ACCGCAGGGC 
AGCCAGAACT CGCTGAACAC CGTGAGCTCA GGCAGCGGCA GCACCAGCGG CATTGGCAGT 
GGTGGTGGCG GAGGCAGCGG CGTGGTGAGC GCTGTGCTGC AGCCATATGA TGTGGAGATT 
CGGCGTGGGG AGAACGAGGG CTTTGGGTTT GTCATCGTGT CCTCCGTGAG CAGGCCTGAA 
GCGGGCACCA CCTTCGGTCG GATTATTGAG GGGAGCCCTG CTGACCGCTG TGGCAAGCTG 
AAAGTAGGAG ACCGGATCTT GGCGGTAAAT GGATGTTCCA TCACCAACAA ATCCCATTCT 
GACATTGTCA ACCTAATCAA AGAAGCAGGC AACACAGTGA CTCTCCGCAT CATCCCCGGG 
GATGAGTCCT CCAATGCCAC ACTGCTGACT AATGCGGAGA AGATTGCCAC CATCACCACC 
ACTCATGCCC CCTCACAACA GGGGACCCAG GAAACAAGGA CCACCACCAA ACCAAAGCCG 
GATTCTCAGT TTGAGTTCAA AGGACCGCAG GCTACACAGG AGCAAGATTT TTACACTGTG 
GAATTGGAAA GAGGAGCCAA GGGATTTGGC TTTAGTCTTC GAGGAGGGCG AGAGTATAAC 
ATGGACCTTT ATGTTCTGCG CTTAGCAGAG GATGGTCCTG CAGAAAGATG CGGAAAGATG 
AGGATTGGCG ATGAAATTCT AGAGATCAAT GGTGAGACCA CCAAAAACAT GAAACACTCT 
CGGGCCATAG AACTGATCAA GAATGGCGGC CGCAGAGTCC GTCTGTTTCT GAGGCGGGGA 
GACGGCTCGG TCCCAGAATA TGACCCCAGC AGCGACAGGA ACGGCCCCTC CACCGGTGCA 
CAAGGTGTTC CGGAAGTGAG GCTGGGGCCA CCGGACCACA GATCACATCC AGCCTTGGAG 
TCCAGTTACC CACCCGATCT TCACAAATCA TCACAACATG CCGAAAAGCG AGCACACGCG 
AAGGATCCGA AAGGCAACAG GGAGCACAGC GGACAGCCCA ACGAACATCA CACTTGGAAC 
GGAACTTCTA GAAAACCCGA CAGTGGGGCA TGCCGGCCCA AAGACCGGCC ACCCGACACA 
TGGAGAGAGG CGCAGCCGGA ATGGACAGCC ACCAATGGCC CAAAGAGGAG GTCTCCGGAG 
AAGCGCAGGG AAGGCACCCG CAGTGCTGAC AACACTTTGG AAAGAAGGGA GAAGCATGAG 
AAGAGAAGAG AGATGTCTCC TGAGAGGAAG CGAGAGCGGT CACCCACCCG GAGAAAAGAC 
AGCTCCCCGA GCCGCCGGCG GAGGTCCCTC GAAAGACTCC TGGATCAAAG AAGGTCCCCA 
GAGCGCAGAA GAGGGGGCTC GCCCGAGAGG AGAGCAAAGT CCACCGACAG GAGGAGGGCC 
AGGTCCCCAG AGCGCAGGCG AGAGCGGTCA CTGGACAAAA GGAACCGAGA CGACAAAGTT 
GGCCACCGAG AAAGGGAGGA GGCTGGTCTG AAGCTGGAAG CCGGGAGAAG CCCCAGAAAT 
CCCCCAGAGC AGAGAAGGCG GCCTTACAAA GAATGTAGCA CCGACCTCAG CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Magi1 ( XM_006236929.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236991.1 Rattus norvegicus membrane associated guanylate kinase, WW and PDZ domain containing 1 (Magi1), transcript variant X4, mRNA.

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CloneID	ORa27497
Clone ID Related Accession (Same CDS sequence)	XM_006236929.3
Accession Version	XM_006236929.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006236929.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAG TGATCCAGAA GAAGAACCAC TGGACTGGCC GCGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GCATGGGGAG 
TTTCCGTACG TGGGGGCGGT GGCGGCGGCC GAGGCGGCGG GGCTTCCCGG CGGTGGCGAG 
GGACCGAAGC TGGCTGAAGG TGAGCTGCTG CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC 
TTGCCCCGCT ATGACGTGCT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA 
GCCGTCAGAC AAGGAGGAAG GCTCAACAAG GACCTGCGAC ATTTCCTCAA CCAACGGTTC 
CAGAAGGGGT CTCCGGATCA TGAACTCCAG CAGACCATAA GGGACAACCT CTACCGCCAT 
GCCGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAAG TGCCTGGCGT CGATTACAGC 
TTTCTGACTG TGAAGGAGTT CCTGGACCTC GAGCAGAGTG GGACCCTGTT GGAAGTCGGC 
ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCCA GCCAGCCAGT CAGTGGGAAA 
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CCAAGCAGTC GACCCCTAAG 
CGAACAAAAT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACACGGA GAATGAGGAA 
GAGGAGGATG TTCCTGAAAT GAACAGTAGC TTCACAGCCG ACTCTGGAGA CCAGGACGAG 
CCCACTCTCC AAGAAGCGAC GCTCCCGCCT GTGAATAGTA GCGCCCTTGC TGCTCCCATC 
ACGGACCCTT CTCAGAAGTT CCCTCAGTAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT 
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC 
CACAACACGA AAACAACATC ATGGTTAGAC CCTCGGTGCC TGAACAAACA GCAGAAGCCT 
CTGGAAGAAT GTGAAGATGA TGAGCTGCCT GCTGGCTGGG AAAAGATTGA AGACCCTGTC 
TACGGTGTCT ACTATGTAGA CCACATCAAC AGGAAGACGC AATATGAAAA CCCAGTCCTA 
GAAGCCAAAA GGAAGAGACA GCTTGAACAA CAGCAGCAGC AGCAGCAGCA CCAGCAGCAG 
CCGCAGCAGC CTCAGCCACC GCAGCCAGAA GAGTGGACAG AAGATCATGC ATCTGTTGTG 
CCTCCTGTTG CTCCTTCCCA TCCCCCAAGC AACCCGGAGC CAGCCAGGGA AGCTCCACTT 
CAGGGCAAAC CTTTTTTTAC AAGAAACCCT TCTGAGCTGA AAGGCAAGTT CATTCACACA 
AAGCTACGGA AAAGCAGTCG AGGCTTTGGC TTCACGGTGG TTGGAGGGGA CGAACCTGAT 
GAGTTCCTGC AGATCAAGAG CCTTGTCCTA GATGGTCCTG CTGCACTGGA TGGCAAGATG 
GAGACAGGAG ATGTAATCGT CAGTGTGAAT GACACCTGTG TTTTGGGACA CACACATGCT 
CAAGTTGTGA AAATTTTCCA GTCCATTCCC ATTGGTGCCA GTGTGGACCT TGAACTCTGC 
AGAGGTTATC CATTGCCTTT TGACCCGGAT GACCCTAACA CAAGTTTAGT GACCTCGGTG 
GCCATTTTGG ACAAAGAACC AATAATTGTA AACGGACAAG AGACCTATGA TTCACCAGCA 
AGCCACAGTA GTAAAACGGG CAAAGTCAGC AACATGAAGG ATGCCAGGCC AAGCAGCCCC 
GCAGACGTGG CTTCCAACAG TTCTCATGGG TATCCCAACG ACACAGTCTC CTTGGCTTCC 
TCCATAGCCA CCCAGCCAGA GCTAATAACC GTTCACATAG TCAAAGGGCC AATGGGATTT 
GGCTTTACGA TCGCAGATAG TCCCGGTGGG GGTGGCCAAA GAGTGAAGCA GATTGTTGAC 
AGTCCGCGCT GCAGAGGCCT CAAAGAAGGG GATCTGATTG TGGAGGTGAA TAAGAGGAAC 
GTGCAGGCCC TGACGCACAA TCAAGTCGTG GATATGCTGA TTGAGTGTCC AAAGGGAAGT 
GAGGTCACAC TGTTGGTGCA GCGAGGAGGG CTACCAGTTC CCAAGAAGAG TCCAAAGTCG 
CAGCCACTGG AGAGGAAAGA CAGCCAGAAC AGCTCCCAGC ACAGTGTGTC CAGCCTCCGG 
AGCCTGCACA CAGCTTCCCC GAGCCACAGT GCACAGGTGC TCCCTGAGTA CCCACCTGCA 
GACGTCCCTG CTCCAGATCA GACCGACAGC TCTGGGCAGA AAAAGCCAGA TCCCTTTAAA 
ATCTGGGCCC AGTCGAGGAG CATGTATGAA AACCGACCTA TGTCACCTTC GCCTGCGTCA 
GGACTGAGCA AGGGTGAAAG AGACAGAGAG ATCAATTCCA CGAATTTTGG AGAATGTCAG 
ATTCCAGATT ACCAGGAGCA GGACATCTTC CTCTGGAGAA AAGAGACCGG TTTTGGATTT 
AGGATTCTGG GTGGAAATGA GCCAGGGGAA CCCATTTATA TCGGTCACAT CGTACCACTG 
GGTGCTGCTG ACACAGACGG CCGCCTGAGG TCTGGAGATG AATTAATCTG TGTGGATGGG 
ACACCAGTAA TTGGGAAATC ACACCAGCTC GTGGTCCAGC TTATGCAACA AGCTGCCAAG 
CAAGGCCACG TCAATCTCAC AGTGAGGCGT AAAGTGGTGT TTACGGTCCC CAAAGCAGAG 
AACGAGGTGC CCTCACCAGC CTCATCGCAC CACAGTAGCA ACCAGCCAGC GTCCCTGACG 
GAAGAGAAGC GCACACCGCA GGGCAGCCAG AACTCGCTGA ACACCGTGAG CTCAGGCAGC 
GGCAGCACCA GCGGCATTGG CAGTGGTGGT GGCGGAGGCA GCGGCGTGGT GAGCGCTGTG 
CTGCAGCCAT ATGATGTGGA GATTCGGCGT GGGGAGAACG AGGGCTTTGG GTTTGTCATC 
GTGTCCTCCG TGAGCAGGCC TGAAGCGGGC ACCACCTTCG GCAATGCATG TGTGGCTATG 
CCTCACAAAA TAGGTCGGAT TATTGAGGGG AGCCCTGCTG ACCGCTGTGG CAAGCTGAAA 
GTAGGAGACC GGATCTTGGC GGTAAATGGA TGTTCCATCA CCAACAAATC CCATTCTGAC 
ATTGTCAACC TAATCAAAGA AGCAGGCAAC ACAGTGACTC TCCGCATCAT CCCCGGGGAT 
GAGTCCTCCA ATGCCACACT GCTGACTAAT GCGGAGAAGA TTGCCACCAT CACCACCACT 
CATGCCCCCT CACAACAGGG GACCCAGGAA ACAAGGACCA CCACCAAACC AAAGCCGGAT 
TCTCAGTTTG AGTTCAAAGG ACCGCAGGCT ACACAGGAGC AAGATTTTTA CACTGTGGAA 
TTGGAAAGAG GAGCCAAGGG ATTTGGCTTT AGTCTTCGAG GAGGGCGAGA GTATAACATG 
GACCTTTATG TTCTGCGCTT AGCAGAGGAT GGTCCTGCAG AAAGATGCGG AAAGATGAGG 
ATTGGCGATG AAATTCTAGA GATCAATGGT GAGACCACCA AAAACATGAA ACACTCTCGG 
GCCATAGAAC TGATCAAGAA TGGCGGCCGC AGAGTCCGTC TGTTTCTGAG GCGGGGAGAC 
GGCTCGGTCC CAGAATATGA CCCCAGCAGC GACAGGAACG GCCCCTCCAC CGGTGCACAA 
GGTGTTCCGG AAGTGAGGCT GGGGCCACCG GACCACAGAT CACATCCAGC CTTGGAGTCC 
AGTTACCCAC CCGATCTTCA CAAATCATCA CAACATGCCG AAAAGCGAGC ACACGCGAAG 
GATCCGAAAG GCAACAGGGA GCACAGCGGA CAGCCCAACG AACATCACAC TTGGAACGGA 
ACTTCTAGAA AACCCGACAG TGGGGCATGC CGGCCCAAAG ACCGGCCACC CGACACATGG 
AGAGAGGCGC AGCCGGAATG GACAGCCACC AATGGCCCAA AGAGGAGGTC TCCGGAGAAG 
CGCAGGGAAG GCACCCGCAG TGCTGACAAC ACTTTGGAAA GAAGGGAGAA GCATGAGAAG 
AGAAGAGAGA TGTCTCCTGA GAGGAAGCGA GAGCGGTCAC CCACCCGGAG AAAAGACAGC 
TCCCCGAGCC GCCGGCGGAG GTCCCTCGAA AGACTCCTGG ATCAAAGAAG GTCCCCAGAG 
CGCAGAAGAG GGGGCTCGCC CGAGAGGAGA GCAAAGTCCA CCGACAGGAG GAGGGCCAGG 
TCCCCAGAGC GCAGGCGAGA GCGGTCACTG GACAAAAGGA ACCGAGACGA CAAAGTTGGC 
CACCGAGAAA GGGAGGAGGC TGGTCTGAAG CTGGAAGCCG GGAGAAGCCC CAGAAATCCC 
CCAGAGCAGA GAAGGCGGCC TTACAAAGAA TGTAGCACCG ACCTCAGCAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236991.1
CDS	604..5037
Translation	MSKVIQKKNHWTGRVHECTVKRGPQGELGVTVLGGAEHGEFPYVGAVAAAEAAGLPGGGEGPKLAEGELLLEVQGVRVSGLPRYDVLGVIDSCKEAVTFKAVRQGGRLNKDLRHFLNQRFQKGSPDHELQQTIRDNLYRHAVPCTTRSPREGEVPGVDYSFLTVKEFLDLEQSGTLLEVGTYEGNYYGTPKPPSQPVSGKVITTDALHSLQSGSKQSTPKRTKSYNDMQNAGIVHTENEEEEDVPEMNSSFTADSGDQDEPTLQEATLPPVNSSALAAPITDPSQKFPQYLPLSAEDNLGPLPENWEMAYTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDELPAGWEKIEDPVYGVYYVDHINRKTQYENPVLEAKRKRQLEQQQQQQQHQQQPQQPQPPQPEEWTEDHASVVPPVAPSHPPSNPEPAREAPLQGKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNDTCVLGHTHAQVVKIFQSIPIGASVDLELCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETYDSPASHSSKTGKVSNMKDARPSSPADVASNSSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKRNVQALTHNQVVDMLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSLRSLHTASPSHSAQVLPEYPPADVPAPDQTDSSGQKKPDPFKIWAQSRSMYENRPMSPSPASGLSKGERDREINSTNFGECQIPDYQEQDIFLWRKETGFGFRILGGNEPGEPIYIGHIVPLGAADTDGRLRSGDELICVDGTPVIGKSHQLVVQLMQQAAKQGHVNLTVRRKVVFTVPKAENEVPSPASSHHSSNQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGVVSAVLQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNATLLTNAEKIATITTTHAPSQQGTQETRTTTKPKPDSQFEFKGPQATQEQDFYTVELERGAKGFGFSLRGGREYNMDLYVLRLAEDGPAERCGKMRIGDEILEINGETTKNMKHSRAIELIKNGGRRVRLFLRRGDGSVPEYDPSSDRNGPSTGAQGVPEVRLGPPDHRSHPALESSYPPDLHKSSQHAEKRAHAKDPKGNREHSGQPNEHHTWNGTSRKPDSGACRPKDRPPDTWREAQPEWTATNGPKRRSPEKRREGTRSADNTLERREKHEKRREMSPERKRERSPTRRKDSSPSRRRRSLERLLDQRRSPERRRGGSPERRAKSTDRRRARSPERRRERSLDKRNRDDKVGHREREEAGLKLEAGRSPRNPPEQRRRPYKECSTDLSI

Target ORF information:

RefSeq Version	XM_006236929.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus membrane associated guanylate kinase, WW and PDZ domain containing 1 (Magi1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236929.3

ORF Insert Sequence:

ATGTCGAAAG TGATCCAGAA GAAGAACCAC TGGACTGGCC GCGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GCATGGGGAG 
TTTCCGTACG TGGGGGCGGT GGCGGCGGCC GAGGCGGCGG GGCTTCCCGG CGGTGGCGAG 
GGACCGAAGC TGGCTGAAGG TGAGCTGCTG CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC 
TTGCCCCGCT ATGACGTGCT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA 
GCCGTCAGAC AAGGAGGAAG GCTCAACAAG GACCTGCGAC ATTTCCTCAA CCAACGGTTC 
CAGAAGGGGT CTCCGGATCA TGAACTCCAG CAGACCATAA GGGACAACCT CTACCGCCAT 
GCCGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAAG TGCCTGGCGT CGATTACAGC 
TTTCTGACTG TGAAGGAGTT CCTGGACCTC GAGCAGAGTG GGACCCTGTT GGAAGTCGGC 
ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCCA GCCAGCCAGT CAGTGGGAAA 
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CCAAGCAGTC GACCCCTAAG 
CGAACAAAAT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACACGGA GAATGAGGAA 
GAGGAGGATG TTCCTGAAAT GAACAGTAGC TTCACAGCCG ACTCTGGAGA CCAGGACGAG 
CCCACTCTCC AAGAAGCGAC GCTCCCGCCT GTGAATAGTA GCGCCCTTGC TGCTCCCATC 
ACGGACCCTT CTCAGAAGTT CCCTCAGTAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT 
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC 
CACAACACGA AAACAACATC ATGGTTAGAC CCTCGGTGCC TGAACAAACA GCAGAAGCCT 
CTGGAAGAAT GTGAAGATGA TGAGCTGCCT GCTGGCTGGG AAAAGATTGA AGACCCTGTC 
TACGGTGTCT ACTATGTAGA CCACATCAAC AGGAAGACGC AATATGAAAA CCCAGTCCTA 
GAAGCCAAAA GGAAGAGACA GCTTGAACAA CAGCAGCAGC AGCAGCAGCA CCAGCAGCAG 
CCGCAGCAGC CTCAGCCACC GCAGCCAGAA GAGTGGACAG AAGATCATGC ATCTGTTGTG 
CCTCCTGTTG CTCCTTCCCA TCCCCCAAGC AACCCGGAGC CAGCCAGGGA AGCTCCACTT 
CAGGGCAAAC CTTTTTTTAC AAGAAACCCT TCTGAGCTGA AAGGCAAGTT CATTCACACA 
AAGCTACGGA AAAGCAGTCG AGGCTTTGGC TTCACGGTGG TTGGAGGGGA CGAACCTGAT 
GAGTTCCTGC AGATCAAGAG CCTTGTCCTA GATGGTCCTG CTGCACTGGA TGGCAAGATG 
GAGACAGGAG ATGTAATCGT CAGTGTGAAT GACACCTGTG TTTTGGGACA CACACATGCT 
CAAGTTGTGA AAATTTTCCA GTCCATTCCC ATTGGTGCCA GTGTGGACCT TGAACTCTGC 
AGAGGTTATC CATTGCCTTT TGACCCGGAT GACCCTAACA CAAGTTTAGT GACCTCGGTG 
GCCATTTTGG ACAAAGAACC AATAATTGTA AACGGACAAG AGACCTATGA TTCACCAGCA 
AGCCACAGTA GTAAAACGGG CAAAGTCAGC AACATGAAGG ATGCCAGGCC AAGCAGCCCC 
GCAGACGTGG CTTCCAACAG TTCTCATGGG TATCCCAACG ACACAGTCTC CTTGGCTTCC 
TCCATAGCCA CCCAGCCAGA GCTAATAACC GTTCACATAG TCAAAGGGCC AATGGGATTT 
GGCTTTACGA TCGCAGATAG TCCCGGTGGG GGTGGCCAAA GAGTGAAGCA GATTGTTGAC 
AGTCCGCGCT GCAGAGGCCT CAAAGAAGGG GATCTGATTG TGGAGGTGAA TAAGAGGAAC 
GTGCAGGCCC TGACGCACAA TCAAGTCGTG GATATGCTGA TTGAGTGTCC AAAGGGAAGT 
GAGGTCACAC TGTTGGTGCA GCGAGGAGGG CTACCAGTTC CCAAGAAGAG TCCAAAGTCG 
CAGCCACTGG AGAGGAAAGA CAGCCAGAAC AGCTCCCAGC ACAGTGTGTC CAGCCTCCGG 
AGCCTGCACA CAGCTTCCCC GAGCCACAGT GCACAGGTGC TCCCTGAGTA CCCACCTGCA 
GACGTCCCTG CTCCAGATCA GACCGACAGC TCTGGGCAGA AAAAGCCAGA TCCCTTTAAA 
ATCTGGGCCC AGTCGAGGAG CATGTATGAA AACCGACCTA TGTCACCTTC GCCTGCGTCA 
GGACTGAGCA AGGGTGAAAG AGACAGAGAG ATCAATTCCA CGAATTTTGG AGAATGTCAG 
ATTCCAGATT ACCAGGAGCA GGACATCTTC CTCTGGAGAA AAGAGACCGG TTTTGGATTT 
AGGATTCTGG GTGGAAATGA GCCAGGGGAA CCCATTTATA TCGGTCACAT CGTACCACTG 
GGTGCTGCTG ACACAGACGG CCGCCTGAGG TCTGGAGATG AATTAATCTG TGTGGATGGG 
ACACCAGTAA TTGGGAAATC ACACCAGCTC GTGGTCCAGC TTATGCAACA AGCTGCCAAG 
CAAGGCCACG TCAATCTCAC AGTGAGGCGT AAAGTGGTGT TTACGGTCCC CAAAGCAGAG 
AACGAGGTGC CCTCACCAGC CTCATCGCAC CACAGTAGCA ACCAGCCAGC GTCCCTGACG 
GAAGAGAAGC GCACACCGCA GGGCAGCCAG AACTCGCTGA ACACCGTGAG CTCAGGCAGC 
GGCAGCACCA GCGGCATTGG CAGTGGTGGT GGCGGAGGCA GCGGCGTGGT GAGCGCTGTG 
CTGCAGCCAT ATGATGTGGA GATTCGGCGT GGGGAGAACG AGGGCTTTGG GTTTGTCATC 
GTGTCCTCCG TGAGCAGGCC TGAAGCGGGC ACCACCTTCG GCAATGCATG TGTGGCTATG 
CCTCACAAAA TAGGTCGGAT TATTGAGGGG AGCCCTGCTG ACCGCTGTGG CAAGCTGAAA 
GTAGGAGACC GGATCTTGGC GGTAAATGGA TGTTCCATCA CCAACAAATC CCATTCTGAC 
ATTGTCAACC TAATCAAAGA AGCAGGCAAC ACAGTGACTC TCCGCATCAT CCCCGGGGAT 
GAGTCCTCCA ATGCCACACT GCTGACTAAT GCGGAGAAGA TTGCCACCAT CACCACCACT 
CATGCCCCCT CACAACAGGG GACCCAGGAA ACAAGGACCA CCACCAAACC AAAGCCGGAT 
TCTCAGTTTG AGTTCAAAGG ACCGCAGGCT ACACAGGAGC AAGATTTTTA CACTGTGGAA 
TTGGAAAGAG GAGCCAAGGG ATTTGGCTTT AGTCTTCGAG GAGGGCGAGA GTATAACATG 
GACCTTTATG TTCTGCGCTT AGCAGAGGAT GGTCCTGCAG AAAGATGCGG AAAGATGAGG 
ATTGGCGATG AAATTCTAGA GATCAATGGT GAGACCACCA AAAACATGAA ACACTCTCGG 
GCCATAGAAC TGATCAAGAA TGGCGGCCGC AGAGTCCGTC TGTTTCTGAG GCGGGGAGAC 
GGCTCGGTCC CAGAATATGA CCCCAGCAGC GACAGGAACG GCCCCTCCAC CGGTGCACAA 
GGTGTTCCGG AAGTGAGGCT GGGGCCACCG GACCACAGAT CACATCCAGC CTTGGAGTCC 
AGTTACCCAC CCGATCTTCA CAAATCATCA CAACATGCCG AAAAGCGAGC ACACGCGAAG 
GATCCGAAAG GCAACAGGGA GCACAGCGGA CAGCCCAACG AACATCACAC TTGGAACGGA 
ACTTCTAGAA AACCCGACAG TGGGGCATGC CGGCCCAAAG ACCGGCCACC CGACACATGG 
AGAGAGGCGC AGCCGGAATG GACAGCCACC AATGGCCCAA AGAGGAGGTC TCCGGAGAAG 
CGCAGGGAAG GCACCCGCAG TGCTGACAAC ACTTTGGAAA GAAGGGAGAA GCATGAGAAG 
AGAAGAGAGA TGTCTCCTGA GAGGAAGCGA GAGCGGTCAC CCACCCGGAG AAAAGACAGC 
TCCCCGAGCC GCCGGCGGAG GTCCCTCGAA AGACTCCTGG ATCAAAGAAG GTCCCCAGAG 
CGCAGAAGAG GGGGCTCGCC CGAGAGGAGA GCAAAGTCCA CCGACAGGAG GAGGGCCAGG 
TCCCCAGAGC GCAGGCGAGA GCGGTCACTG GACAAAAGGA ACCGAGACGA CAAAGTTGGC 
CACCGAGAAA GGGAGGAGGC TGGTCTGAAG CTGGAAGCCG GGAGAAGCCC CAGAAATCCC 
CCAGAGCAGA GAAGGCGGCC TTACAAAGAA TGTAGCACCG ACCTCAGCAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Magi1 ( XM_008763133.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761355.1 Rattus norvegicus membrane associated guanylate kinase, WW and PDZ domain containing 1 (Magi1), transcript variant X5, mRNA.

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CloneID	ORa49329
Clone ID Related Accession (Same CDS sequence)	XM_008763133.2
Accession Version	XM_008763133.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763133.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAAAG TGATCCAGAA GAAGAACCAC TGGACTGGCC GCGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GCATGGGGAG 
TTTCCGTACG TGGGGGCGGT GGCGGCGGCC GAGGCGGCGG GGCTTCCCGG CGGTGGCGAG 
GGACCGAAGC TGGCTGAAGG TGAGCTGCTG CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC 
TTGCCCCGCT ATGACGTGCT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA 
GCCGTCAGAC AAGGAGGAAG GCTCAACAAG GACCTGCGAC ATTTCCTCAA CCAACGGTTC 
CAGAAGGGGT CTCCGGATCA TGAACTCCAG CAGACCATAA GGGACAACCT CTACCGCCAT 
GCCGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAAG TGCCTGGCGT CGATTACAGC 
TTTCTGACTG TGAAGGAGTT CCTGGACCTC GAGCAGAGTG GGACCCTGTT GGAAGTCGGC 
ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCCA GCCAGCCAGT CAGTGGGAAA 
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CCAAGCAGTC GACCCCTAAG 
CGAACAAAAT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACACGGA GAATGAGGAA 
GAGGAGGATG TTCCTGAAAT GAACAGTAGC TTCACAGCCG ACTCTGGAGA CCAGGACGAG 
CCCACTCTCC AAGAAGCGAC GCTCCCGCCT GTGAATAGTA GCGCCCTTGC TGCTCCCATC 
ACGGACCCTT CTCAGAAGTT CCCTCAGTAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT 
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC 
CACAACACGA AAACAACATC ATGGTTAGAC CCTCGGTGCC TGAACAAACA GCAGAAGCCT 
CTGGAAGAAT GTGAAGATGA TGAAGGGGTA CACACCGAGG AGCTGGACAG TGAACTAGAG 
CTGCCTGCTG GCTGGGAAAA GATTGAAGAC CCTGTCTACG GTGTCTACTA TGTAGACCAC 
ATCAACAGGA AGACGCAATA TGAAAACCCA GTCCTAGAAG CCAAAAGGAA GAGACAGCTT 
GAACAACAGC AGCAGCAGCA GCAGCACCAG CAGCAGCCGC AGCAGCCTCA GCCACCGCAG 
CCAGAAGAGT GGACAGAAGA TCATGCATCT GTTGTGCCTC CTGTTGCTCC TTCCCATCCC 
CCAAGCAACC CGGAGCCAGC CAGGGAAGCT CCACTTCAGG GCAAACCTTT TTTTACAAGA 
AACCCTTCTG AGCTGAAAGG CAAGTTCATT CACACAAAGC TACGGAAAAG CAGTCGAGGC 
TTTGGCTTCA CGGTGGTTGG AGGGGACGAA CCTGATGAGT TCCTGCAGAT CAAGAGCCTT 
GTCCTAGATG GTCCTGCTGC ACTGGATGGC AAGATGGAGA CAGGAGATGT AATCGTCAGT 
GTGAATGACA CCTGTGTTTT GGGACACACA CATGCTCAAG TTGTGAAAAT TTTCCAGTCC 
ATTCCCATTG GTGCCAGTGT GGACCTTGAA CTCTGCAGAG GTTATCCATT GCCTTTTGAC 
CCGGATGACC CTAACACAAG TTTAGTGACC TCGGTGGCCA TTTTGGACAA AGAACCAATA 
ATTGTAAACG GACAAGAGAC CTATGATTCA CCAGCAAGCC ACAGTAGTAA AACGGGCAAA 
GTCAGCAACA TGAAGGATGC CAGGCCAAGC AGCCCCGCAG ACGTGGCTTC CAACAGTTCT 
CATGGGTATC CCAACGACAC AGTCTCCTTG GCTTCCTCCA TAGCCACCCA GCCAGAGCTA 
ATAACCGTTC ACATAGTCAA AGGGCCAATG GGATTTGGCT TTACGATCGC AGATAGTCCC 
GGTGGGGGTG GCCAAAGAGT GAAGCAGATT GTTGACAGTC CGCGCTGCAG AGGCCTCAAA 
GAAGGGGATC TGATTGTGGA GGTGAATAAG AGGAACGTGC AGGCCCTGAC GCACAATCAA 
GTCGTGGATA TGCTGATTGA GTGTCCAAAG GGAAGTGAGG TCACACTGTT GGTGCAGCGA 
GGAGGGCTAC CAGTTCCCAA GAAGAGTCCA AAGTCGCAGC CACTGGAGAG GAAAGACAGC 
CAGAACAGCT CCCAGCACAG TGTGTCCAGC CTCCGGAGCC TGCACACAGC TTCCCCGAGC 
CACAGTGCAC AGGTGCTCCC TGAGTACCCA CCTGCAGACG TCCCTGCTCC AGATCAGACC 
GACAGCTCTG GGCAGAAAAA GCCAGATCCC TTTAAAATCT GGGCCCAGTC GAGGAGCATG 
TATGAAAACC GACTTCCAGA TTACCAGGAG CAGGACATCT TCCTCTGGAG AAAAGAGACC 
GGTTTTGGAT TTAGGATTCT GGGTGGAAAT GAGCCAGGGG AACCCATTTA TATCGGTCAC 
ATCGTACCAC TGGGTGCTGC TGACACAGAC GGCCGCCTGA GGTCTGGAGA TGAATTAATC 
TGTGTGGATG GGACACCAGT AATTGGGAAA TCACACCAGC TCGTGGTCCA GCTTATGCAA 
CAAGCTGCCA AGCAAGGCCA CGTCAATCTC ACAGTGAGGC GTAAAGTGGT GTTTACGGTC 
CCCAAAGCAG AGAACGAGGT GCCCTCACCA GCCTCATCGC ACCACAGTAG CAACCAGCCA 
GCGTCCCTGA CGGAAGAGAA GCGCACACCG CAGGGCAGCC AGAACTCGCT GAACACCGTG 
AGCTCAGGCA GCGGCAGCAC CAGCGGCATT GGCAGTGGTG GTGGCGGAGG CAGCGGCGTG 
GTGAGCGCTG TGCTGCAGCC ATATGATGTG GAGATTCGGC GTGGGGAGAA CGAGGGCTTT 
GGGTTTGTCA TCGTGTCCTC CGTGAGCAGG CCTGAAGCGG GCACCACCTT CGGCAATGCA 
TGTGTGGCTA TGCCTCACAA AATAGGTCGG ATTATTGAGG GGAGCCCTGC TGACCGCTGT 
GGCAAGCTGA AAGTAGGAGA CCGGATCTTG GCGGTAAATG GATGTTCCAT CACCAACAAA 
TCCCATTCTG ACATTGTCAA CCTAATCAAA GAAGCAGGCA ACACAGTGAC TCTCCGCATC 
ATCCCCGGGG ATGAGTCCTC CAATGCCACA CTGCTGACTA ATGCGGAGAA GATTGCCACC 
ATCACCACCA CTCATGCCCC CTCACAACAG GGGACCCAGG AAACAAGGAC CACCACCAAA 
CCAAAGCCGG ATTCTCAGTT TGAGTTCAAA GGACCGCAGG CTACACAGGA GCAAGATTTT 
TACACTGTGG AATTGGAAAG AGGAGCCAAG GGATTTGGCT TTAGTCTTCG AGGAGGGCGA 
GAGTATAACA TGGACCTTTA TGTTCTGCGC TTAGCAGAGG ATGGTCCTGC AGAAAGATGC 
GGAAAGATGA GGATTGGCGA TGAAATTCTA GAGATCAATG GTGAGACCAC CAAAAACATG 
AAACACTCTC GGGCCATAGA ACTGATCAAG AATGGCGGCC GCAGAGTCCG TCTGTTTCTG 
AGGCGGGGAG ACGGCTCGGT CCCAGAATAT GACCCCAGCA GCGACAGGAA CGGCCCCTCC 
ACCGGTGCAC AAGGTGTTCC GGAAGTGAGG CTGGGGCCAC CGGACCACAG ATCACATCCA 
GCCTTGGAGT CCAGTTACCC ACCCGATCTT CACAAATCAT CACAACATGC CGAAAAGCGA 
GCACACGCGA AGGATCCGAA AGGCAACAGG GAGCACAGCG GACAGCCCAA CGAACATCAC 
ACTTGGAACG GAACTTCTAG AAAACCCGAC AGTGGGGCAT GCCGGCCCAA AGACCGGCCA 
CCCGACACAT GGAGAGAGGC GCAGCCGGAA TGGACAGCCA CCAATGGCCC AAAGAGGAGG 
TCTCCGGAGA AGCGCAGGGA AGGCACCCGC AGTGCTGACA ACACTTTGGA AAGAAGGGAG 
AAGCATGAGA AGAGAAGAGA GATGTCTCCT GAGAGGAAGC GAGAGCGGTC ACCCACCCGG 
AGAAAAGACA GCTCCCCGAG CCGCCGGCGG AGGTCCCTCG AAAGACTCCT GGATCAAAGA 
AGGTCCCCAG AGCGCAGAAG AGGGGGCTCG CCCGAGAGGA GAGCAAAGTC CACCGACAGG 
AGGAGGGCCA GGTCCCCAGA GCGCAGGCGA GAGCGGTCAC TGGACAAAAG GAACCGAGAC 
GACAAAGTTG GCCACCGAGA AAGGGAGGAG GCTGGTCTGA AGCTGGAAGC CGGGAGAAGC 
CCCAGAAATC CCCCAGAGCA GAGAAGGCGG CCTTACAAAG AATGTAGCAC CGACCTCAGC 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761355.1
CDS	604..4989
Translation	MSKVIQKKNHWTGRVHECTVKRGPQGELGVTVLGGAEHGEFPYVGAVAAAEAAGLPGGGEGPKLAEGELLLEVQGVRVSGLPRYDVLGVIDSCKEAVTFKAVRQGGRLNKDLRHFLNQRFQKGSPDHELQQTIRDNLYRHAVPCTTRSPREGEVPGVDYSFLTVKEFLDLEQSGTLLEVGTYEGNYYGTPKPPSQPVSGKVITTDALHSLQSGSKQSTPKRTKSYNDMQNAGIVHTENEEEEDVPEMNSSFTADSGDQDEPTLQEATLPPVNSSALAAPITDPSQKFPQYLPLSAEDNLGPLPENWEMAYTENGEVYFIDHNTKTTSWLDPRCLNKQQKPLEECEDDEGVHTEELDSELELPAGWEKIEDPVYGVYYVDHINRKTQYENPVLEAKRKRQLEQQQQQQQHQQQPQQPQPPQPEEWTEDHASVVPPVAPSHPPSNPEPAREAPLQGKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNDTCVLGHTHAQVVKIFQSIPIGASVDLELCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETYDSPASHSSKTGKVSNMKDARPSSPADVASNSSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKRNVQALTHNQVVDMLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSLRSLHTASPSHSAQVLPEYPPADVPAPDQTDSSGQKKPDPFKIWAQSRSMYENRLPDYQEQDIFLWRKETGFGFRILGGNEPGEPIYIGHIVPLGAADTDGRLRSGDELICVDGTPVIGKSHQLVVQLMQQAAKQGHVNLTVRRKVVFTVPKAENEVPSPASSHHSSNQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGVVSAVLQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNATLLTNAEKIATITTTHAPSQQGTQETRTTTKPKPDSQFEFKGPQATQEQDFYTVELERGAKGFGFSLRGGREYNMDLYVLRLAEDGPAERCGKMRIGDEILEINGETTKNMKHSRAIELIKNGGRRVRLFLRRGDGSVPEYDPSSDRNGPSTGAQGVPEVRLGPPDHRSHPALESSYPPDLHKSSQHAEKRAHAKDPKGNREHSGQPNEHHTWNGTSRKPDSGACRPKDRPPDTWREAQPEWTATNGPKRRSPEKRREGTRSADNTLERREKHEKRREMSPERKRERSPTRRKDSSPSRRRRSLERLLDQRRSPERRRGGSPERRAKSTDRRRARSPERRRERSLDKRNRDDKVGHREREEAGLKLEAGRSPRNPPEQRRRPYKECSTDLSI

Target ORF information:

RefSeq Version	XM_008763133.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus membrane associated guanylate kinase, WW and PDZ domain containing 1 (Magi1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763133.2

ORF Insert Sequence:

ATGTCGAAAG TGATCCAGAA GAAGAACCAC TGGACTGGCC GCGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GCATGGGGAG 
TTTCCGTACG TGGGGGCGGT GGCGGCGGCC GAGGCGGCGG GGCTTCCCGG CGGTGGCGAG 
GGACCGAAGC TGGCTGAAGG TGAGCTGCTG CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC 
TTGCCCCGCT ATGACGTGCT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA 
GCCGTCAGAC AAGGAGGAAG GCTCAACAAG GACCTGCGAC ATTTCCTCAA CCAACGGTTC 
CAGAAGGGGT CTCCGGATCA TGAACTCCAG CAGACCATAA GGGACAACCT CTACCGCCAT 
GCCGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAAG TGCCTGGCGT CGATTACAGC 
TTTCTGACTG TGAAGGAGTT CCTGGACCTC GAGCAGAGTG GGACCCTGTT GGAAGTCGGC 
ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCCA GCCAGCCAGT CAGTGGGAAA 
GTGATCACGA CGGATGCCTT GCACAGCCTT CAGTCTGGCT CCAAGCAGTC GACCCCTAAG 
CGAACAAAAT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACACGGA GAATGAGGAA 
GAGGAGGATG TTCCTGAAAT GAACAGTAGC TTCACAGCCG ACTCTGGAGA CCAGGACGAG 
CCCACTCTCC AAGAAGCGAC GCTCCCGCCT GTGAATAGTA GCGCCCTTGC TGCTCCCATC 
ACGGACCCTT CTCAGAAGTT CCCTCAGTAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT 
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC 
CACAACACGA AAACAACATC ATGGTTAGAC CCTCGGTGCC TGAACAAACA GCAGAAGCCT 
CTGGAAGAAT GTGAAGATGA TGAAGGGGTA CACACCGAGG AGCTGGACAG TGAACTAGAG 
CTGCCTGCTG GCTGGGAAAA GATTGAAGAC CCTGTCTACG GTGTCTACTA TGTAGACCAC 
ATCAACAGGA AGACGCAATA TGAAAACCCA GTCCTAGAAG CCAAAAGGAA GAGACAGCTT 
GAACAACAGC AGCAGCAGCA GCAGCACCAG CAGCAGCCGC AGCAGCCTCA GCCACCGCAG 
CCAGAAGAGT GGACAGAAGA TCATGCATCT GTTGTGCCTC CTGTTGCTCC TTCCCATCCC 
CCAAGCAACC CGGAGCCAGC CAGGGAAGCT CCACTTCAGG GCAAACCTTT TTTTACAAGA 
AACCCTTCTG AGCTGAAAGG CAAGTTCATT CACACAAAGC TACGGAAAAG CAGTCGAGGC 
TTTGGCTTCA CGGTGGTTGG AGGGGACGAA CCTGATGAGT TCCTGCAGAT CAAGAGCCTT 
GTCCTAGATG GTCCTGCTGC ACTGGATGGC AAGATGGAGA CAGGAGATGT AATCGTCAGT 
GTGAATGACA CCTGTGTTTT GGGACACACA CATGCTCAAG TTGTGAAAAT TTTCCAGTCC 
ATTCCCATTG GTGCCAGTGT GGACCTTGAA CTCTGCAGAG GTTATCCATT GCCTTTTGAC 
CCGGATGACC CTAACACAAG TTTAGTGACC TCGGTGGCCA TTTTGGACAA AGAACCAATA 
ATTGTAAACG GACAAGAGAC CTATGATTCA CCAGCAAGCC ACAGTAGTAA AACGGGCAAA 
GTCAGCAACA TGAAGGATGC CAGGCCAAGC AGCCCCGCAG ACGTGGCTTC CAACAGTTCT 
CATGGGTATC CCAACGACAC AGTCTCCTTG GCTTCCTCCA TAGCCACCCA GCCAGAGCTA 
ATAACCGTTC ACATAGTCAA AGGGCCAATG GGATTTGGCT TTACGATCGC AGATAGTCCC 
GGTGGGGGTG GCCAAAGAGT GAAGCAGATT GTTGACAGTC CGCGCTGCAG AGGCCTCAAA 
GAAGGGGATC TGATTGTGGA GGTGAATAAG AGGAACGTGC AGGCCCTGAC GCACAATCAA 
GTCGTGGATA TGCTGATTGA GTGTCCAAAG GGAAGTGAGG TCACACTGTT GGTGCAGCGA 
GGAGGGCTAC CAGTTCCCAA GAAGAGTCCA AAGTCGCAGC CACTGGAGAG GAAAGACAGC 
CAGAACAGCT CCCAGCACAG TGTGTCCAGC CTCCGGAGCC TGCACACAGC TTCCCCGAGC 
CACAGTGCAC AGGTGCTCCC TGAGTACCCA CCTGCAGACG TCCCTGCTCC AGATCAGACC 
GACAGCTCTG GGCAGAAAAA GCCAGATCCC TTTAAAATCT GGGCCCAGTC GAGGAGCATG 
TATGAAAACC GACTTCCAGA TTACCAGGAG CAGGACATCT TCCTCTGGAG AAAAGAGACC 
GGTTTTGGAT TTAGGATTCT GGGTGGAAAT GAGCCAGGGG AACCCATTTA TATCGGTCAC 
ATCGTACCAC TGGGTGCTGC TGACACAGAC GGCCGCCTGA GGTCTGGAGA TGAATTAATC 
TGTGTGGATG GGACACCAGT AATTGGGAAA TCACACCAGC TCGTGGTCCA GCTTATGCAA 
CAAGCTGCCA AGCAAGGCCA CGTCAATCTC ACAGTGAGGC GTAAAGTGGT GTTTACGGTC 
CCCAAAGCAG AGAACGAGGT GCCCTCACCA GCCTCATCGC ACCACAGTAG CAACCAGCCA 
GCGTCCCTGA CGGAAGAGAA GCGCACACCG CAGGGCAGCC AGAACTCGCT GAACACCGTG 
AGCTCAGGCA GCGGCAGCAC CAGCGGCATT GGCAGTGGTG GTGGCGGAGG CAGCGGCGTG 
GTGAGCGCTG TGCTGCAGCC ATATGATGTG GAGATTCGGC GTGGGGAGAA CGAGGGCTTT 
GGGTTTGTCA TCGTGTCCTC CGTGAGCAGG CCTGAAGCGG GCACCACCTT CGGCAATGCA 
TGTGTGGCTA TGCCTCACAA AATAGGTCGG ATTATTGAGG GGAGCCCTGC TGACCGCTGT 
GGCAAGCTGA AAGTAGGAGA CCGGATCTTG GCGGTAAATG GATGTTCCAT CACCAACAAA 
TCCCATTCTG ACATTGTCAA CCTAATCAAA GAAGCAGGCA ACACAGTGAC TCTCCGCATC 
ATCCCCGGGG ATGAGTCCTC CAATGCCACA CTGCTGACTA ATGCGGAGAA GATTGCCACC 
ATCACCACCA CTCATGCCCC CTCACAACAG GGGACCCAGG AAACAAGGAC CACCACCAAA 
CCAAAGCCGG ATTCTCAGTT TGAGTTCAAA GGACCGCAGG CTACACAGGA GCAAGATTTT 
TACACTGTGG AATTGGAAAG AGGAGCCAAG GGATTTGGCT TTAGTCTTCG AGGAGGGCGA 
GAGTATAACA TGGACCTTTA TGTTCTGCGC TTAGCAGAGG ATGGTCCTGC AGAAAGATGC 
GGAAAGATGA GGATTGGCGA TGAAATTCTA GAGATCAATG GTGAGACCAC CAAAAACATG 
AAACACTCTC GGGCCATAGA ACTGATCAAG AATGGCGGCC GCAGAGTCCG TCTGTTTCTG 
AGGCGGGGAG ACGGCTCGGT CCCAGAATAT GACCCCAGCA GCGACAGGAA CGGCCCCTCC 
ACCGGTGCAC AAGGTGTTCC GGAAGTGAGG CTGGGGCCAC CGGACCACAG ATCACATCCA 
GCCTTGGAGT CCAGTTACCC ACCCGATCTT CACAAATCAT CACAACATGC CGAAAAGCGA 
GCACACGCGA AGGATCCGAA AGGCAACAGG GAGCACAGCG GACAGCCCAA CGAACATCAC 
ACTTGGAACG GAACTTCTAG AAAACCCGAC AGTGGGGCAT GCCGGCCCAA AGACCGGCCA 
CCCGACACAT GGAGAGAGGC GCAGCCGGAA TGGACAGCCA CCAATGGCCC AAAGAGGAGG 
TCTCCGGAGA AGCGCAGGGA AGGCACCCGC AGTGCTGACA ACACTTTGGA AAGAAGGGAG 
AAGCATGAGA AGAGAAGAGA GATGTCTCCT GAGAGGAAGC GAGAGCGGTC ACCCACCCGG 
AGAAAAGACA GCTCCCCGAG CCGCCGGCGG AGGTCCCTCG AAAGACTCCT GGATCAAAGA 
AGGTCCCCAG AGCGCAGAAG AGGGGGCTCG CCCGAGAGGA GAGCAAAGTC CACCGACAGG 
AGGAGGGCCA GGTCCCCAGA GCGCAGGCGA GAGCGGTCAC TGGACAAAAG GAACCGAGAC 
GACAAAGTTG GCCACCGAGA AAGGGAGGAG GCTGGTCTGA AGCTGGAAGC CGGGAGAAGC 
CCCAGAAATC CCCCAGAGCA GAGAAGGCGG CCTTACAAAG AATGTAGCAC CGACCTCAGC 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Magi1 ( NM_001030045.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001025216.1 Rattus norvegicus membrane associated guanylate kinase, WW and PDZ domain containing 1 (Magi1), mRNA.

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CloneID	ORa08314
Clone ID Related Accession (Same CDS sequence)	NM_001030045.1
Accession Version	NM_001030045.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Rattus norvegicus(Norway rat)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY598952.1. On Aug 5, 2005 this sequence version replaced XM_575611.1. ##Evidence-Data-START## Transcript exon combination :: AY598952.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCGAAAG TGATCCAGAA GAAGAACCAC TGGACTGGCC GCGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GCATGGGGAG 
TTTCCGTACG TGGGGGCGGT GGCGGCGGCC GAGGCGGCGG GGCTTCCCGG CGGTGGCGAG 
GGGCCGAAGC TGGCTGAAGG TGAGCTGCTG CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC 
TTGCCCCGCT ATGACGTGCT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA 
GCCGTCAGAC AAGGAGGAAG GCTCAACAAG GACCTACGAC ATTTCCTCAA CCAACGGTTC 
CAGAAGGGGT CTCCAGATCA TGAGCTCCAG CAGACCATAA GGGACAACCT CTACCGCCAT 
GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAAG TGCCTGGCGT CGATTACAGC 
TTTCTGACTG TGAAGGAGTT CCTGGACCTC GAGCAGAGTG GGACCCTGTT GGAAGTCGGC 
ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCCA GCCAGCCAGT CAGTGGGAAA 
GTGATCACGA CGGATGCCTT GCACAGCCTG CAGTCTGGCT CCAAACAGTC GACCCCTAAG 
CGAACAAAAT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACACGGA GAATGAGGAA 
GAGGAGGATG TTCCTGAAAT GAACAGTAGC TTCACAGCCG ACTCTGGAGA CCAGGACGAG 
CCCACTCTCC AAGAAGCGAC GCTCCCGCCT GTGAATAGTA GCGCCCTTGC TGCTCCCATC 
ACGGACCCTT CTCAGAAGTT CCCTCAGTAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT 
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC 
CACAACGCGA AAACAACATC ATGGTTAGAC CCTCGGTGCC TGAACAAACA GCAGAAGCCT 
CTGGAAGAAT GTGAAGATGA TGAAGGGGTA CACACCGAGG AGCTGGACAG TGAACTAGAG 
CTGCCTGCTG GCTGGGAAAA GATTGAAGAC CCTGTCTACG GTGTCTACTA TGTAGACCAC 
ATCAACAGGA AGACGCAATA TGAAAACCCA GTCCTAGAAG CCAAAAGGAA GAGACAGCTT 
GAACAACAGC AGCAGCAGCA GCAGCACCAG CAGCAGCCGC AGCAGCCTCA GCCACCGCAG 
CCAGAAGAGT GGACAGAAGA TCATGCATCT GTTGTGCCTC CTGTTGCTCC TTCCCATCCC 
CCAAGCAACC CGGAGCCAGC CAGGGAAGCT CCACTTCAGG GCAAACCTTT TTTTACAAGA 
AACCCTTCTG AGCTGGAAGG CAAGTTCATT CACACAAAGC TACGGAAAAG CAGTCGAGGC 
TTTGGCTTCA CGGTGGTTGG AGGGGACGAA CCTGATGAGT TCCTGCAGAT CAAGAGCCTT 
GTCCTAGATG GTCCTGCTGC ACTGGATGGC AAGATGGAGA CAGGAGATGT AATCGTCAGT 
GTGAATGACA CCTGTGTTTT GGGACACACA CATGCTCAAG TTGTGAAAAT TTTCCAGTCC 
ATTCCCATTG GTGCCAGTGT GGACCTTGAA CTCTGCAGAG GTTATCCATT GCCTTTTGAC 
CCGGATGACC CTAACACAAG TTTAGTGACC TCGGTGGCCA TTTTGGACAA AGAACCAATA 
ATTGTAAACG GACAAGAGAC CTATGATTCA CCAGCAAGCC ACAGTAGTAA AACGGGCAAA 
GTCAGCAACA TGAAGGATGC CAGGCCAAGC AGCCCCGCAG ACGTGGCTTC CAACAGTTCT 
CATGGGTATC CCAACGACAC AGTCTCCTTG GCTTCCTCCA TAGCCACCCA GCCAGAGCTA 
ATAACCGTTC ACATAGTCAA AGGGCCAATG GGATTTGGCT TTACGATCGC AGATAGTCCC 
GGTGGGGGTG GCCAAAGAGT GAAGCAGATT GTTGACAGTC CGCGCTGCAG AGGCCTCAAA 
GAAGGGGATC TGATTGTGGA GGTGAATAAG AGGAACGTGC AGGCCCTGAC GCACAATCAA 
GTCGTGGATA TGCTGATTGA GTGTCCAAAG GGAAGTGAGG TCACACTGTT GGTGCAGCGA 
GGAGGGCTAC CAGTTCCCAA GAAGAGTCCA AAGTCGCAGC CACTGGAGAG GAAAGACAGC 
CAGAACAGCT CCCAGCACAG TGTGTCCAGC CTCCGGAGCC TGCACACAGC TTCCCCGAGC 
CACAGTGCAC AGGTGCTCCC TGAGTACCCA CCTGCAGACG TCCCTGCTCC AGATCAGACC 
GACAGCTCTG GGCAGAAAAA GCCAGATCCC TTTAAAATCT GGGCCCAGTC GAGGAGCATG 
TATGAAGACC GACCTATGTC ACCTTCGCCT GCGTCAGGAC TGAGCAAGGG TGAAAGAGAC 
AGAGAGATCA ATTCCACGAA TTTTGGAGAA TGTCAGATTC CAGATTACCA GGAGCAGGAC 
ATCTTCCTCT GGAGAAAAGA GACCGGTTTT GGATTTAGGA TTCTGGGTGG AAATGAGCCA 
GGGGAACCCA TTTATATCGG TCACATCGTA CCACTGGGTG CTGCTGACAC AGACGGCCGC 
CTGAGGTCTG GAGATGAATT AATCTGTGTG GATGGGACAC CAGTAATTGG GAAATCACAC 
CAGCTCGTGG TCCAGCTTAT GCAACAAGCT GCCAAGCAAG GCCACGTCAA TCTCACAGTG 
AGGCGTAAAG TGGTGTTTAC GGTCCCCAAA GCAGAGAACG AGGTGCCCTC ACCAGCCTCA 
TCGCACCACA GTAGCAACCA GCCAGCGTCC CTGACGGAAG AGAAGCGCAC ACCGCAGGGC 
AGCCAGAACT CGCTGAACAC CGTGAGCTCA GGCAGCGGCA GCACCAGCGG CATTGGCAGT 
GGTGGTGGCG GAGGCAGCGG CGTGGTGAGC GCTGTGCTGC AGCCATATGA TGTGGAGATT 
CGGCGTGGGG AGAACGAGGG CTTTGGGTTT GTCATCGTGT CCTCCGTGAG CAGGCCCGAA 
GCGGGCACCA CCTTCGCAGG CAATGCATGT GTGGCTATGC CTCACAAAAT AGGTCGGATT 
ATTGAGGGGA GCCCTGCTGA CCGCTGTGGC AAGCTGAAAG TAGGAGACCG GATCTTGGCG 
GTAAATGGAT GTTCCATCAC CAACAAATCC CATTCTGACA TTGTCAACCT AATCAAAGAA 
GCAGGCAACA CAGTGACTCT CCGCATCATC CCCGGGGATG AGTCCTCCAA TGCCACACTG 
CTGACTAATG CGGAGAAGAT TGCCACCATC ACCACCACTC ATGCCCCCTC ACAACAGGGG 
ACCCAGGAAA CAAGGACCAC CACCAAACCA AAGCCGGATT CTCAGTTTGA GTTCAAAGGA 
CCGCAGGCTA CACAGGAGCA AGATTTTTAC ACTGTGGAAT TGGAAAGAGG AGCCAAGGGA 
TTTGGCTTTA GTCTTCGAGG AGGGCGAGAG TATAACATGG ACCTTTATGT TCTGCGCTTA 
GCAGAGGATG GTCCTGCAGA AAGATGCGGA AAGATGAGGA TTGGCGATGA AATTCTAGAG 
ATCAATGGTG AGACCACCAA AAACATGAAA CACTCTCGGG CCATAGAACT GATCAAGAAT 
GGCGGCCGCA GAGTCCGTCT GTTTCTGAGG CGGGGAGACG GCTCGGTCCC AGAATATGGT 
GGGTCAAACT ATGAAAACAT CCCTTCCTTC CCTGGCATGA CTCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025216.1
CDS	1..3768
Translation	MSKVIQKKNHWTGRVHECTVKRGPQGELGVTVLGGAEHGEFPYVGAVAAAEAAGLPGGGEGPKLAEGELLLEVQGVRVSGLPRYDVLGVIDSCKEAVTFKAVRQGGRLNKDLRHFLNQRFQKGSPDHELQQTIRDNLYRHAVPCTTRSPREGEVPGVDYSFLTVKEFLDLEQSGTLLEVGTYEGNYYGTPKPPSQPVSGKVITTDALHSLQSGSKQSTPKRTKSYNDMQNAGIVHTENEEEEDVPEMNSSFTADSGDQDEPTLQEATLPPVNSSALAAPITDPSQKFPQYLPLSAEDNLGPLPENWEMAYTENGEVYFIDHNAKTTSWLDPRCLNKQQKPLEECEDDEGVHTEELDSELELPAGWEKIEDPVYGVYYVDHINRKTQYENPVLEAKRKRQLEQQQQQQQHQQQPQQPQPPQPEEWTEDHASVVPPVAPSHPPSNPEPAREAPLQGKPFFTRNPSELEGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDGKMETGDVIVSVNDTCVLGHTHAQVVKIFQSIPIGASVDLELCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETYDSPASHSSKTGKVSNMKDARPSSPADVASNSSHGYPNDTVSLASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKRNVQALTHNQVVDMLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSLRSLHTASPSHSAQVLPEYPPADVPAPDQTDSSGQKKPDPFKIWAQSRSMYEDRPMSPSPASGLSKGERDREINSTNFGECQIPDYQEQDIFLWRKETGFGFRILGGNEPGEPIYIGHIVPLGAADTDGRLRSGDELICVDGTPVIGKSHQLVVQLMQQAAKQGHVNLTVRRKVVFTVPKAENEVPSPASSHHSSNQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGVVSAVLQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNATLLTNAEKIATITTTHAPSQQGTQETRTTTKPKPDSQFEFKGPQATQEQDFYTVELERGAKGFGFSLRGGREYNMDLYVLRLAEDGPAERCGKMRIGDEILEINGETTKNMKHSRAIELIKNGGRRVRLFLRRGDGSVPEYGGSNYENIPSFPGMTP

Target ORF information:

RefSeq Version	NM_001030045.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus membrane associated guanylate kinase, WW and PDZ domain containing 1 (Magi1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030045.1

ORF Insert Sequence:

ATGTCGAAAG TGATCCAGAA GAAGAACCAC TGGACTGGCC GCGTTCACGA ATGCACCGTG 
AAGCGGGGAC CCCAGGGCGA GCTGGGGGTG ACGGTGCTGG GGGGCGCGGA GCATGGGGAG 
TTTCCGTACG TGGGGGCGGT GGCGGCGGCC GAGGCGGCGG GGCTTCCCGG CGGTGGCGAG 
GGGCCGAAGC TGGCTGAAGG TGAGCTGCTG CTGGAGGTGC AGGGGGTCCG GGTGTCCGGC 
TTGCCCCGCT ATGACGTGCT GGGAGTCATC GACAGCTGCA AGGAGGCCGT CACCTTCAAA 
GCCGTCAGAC AAGGAGGAAG GCTCAACAAG GACCTACGAC ATTTCCTCAA CCAACGGTTC 
CAGAAGGGGT CTCCAGATCA TGAGCTCCAG CAGACCATAA GGGACAACCT CTACCGCCAT 
GCTGTGCCTT GCACAACCCG GTCTCCCAGA GAAGGAGAAG TGCCTGGCGT CGATTACAGC 
TTTCTGACTG TGAAGGAGTT CCTGGACCTC GAGCAGAGTG GGACCCTGTT GGAAGTCGGC 
ACCTATGAAG GAAACTATTA TGGGACACCC AAACCTCCCA GCCAGCCAGT CAGTGGGAAA 
GTGATCACGA CGGATGCCTT GCACAGCCTG CAGTCTGGCT CCAAACAGTC GACCCCTAAG 
CGAACAAAAT CCTACAATGA TATGCAAAAT GCTGGCATAG TCCACACGGA GAATGAGGAA 
GAGGAGGATG TTCCTGAAAT GAACAGTAGC TTCACAGCCG ACTCTGGAGA CCAGGACGAG 
CCCACTCTCC AAGAAGCGAC GCTCCCGCCT GTGAATAGTA GCGCCCTTGC TGCTCCCATC 
ACGGACCCTT CTCAGAAGTT CCCTCAGTAC CTACCTCTTT CTGCAGAGGA TAATTTAGGT 
CCTCTACCTG AAAACTGGGA GATGGCCTAT ACTGAAAATG GAGAAGTCTA TTTTATAGAC 
CACAACGCGA AAACAACATC ATGGTTAGAC CCTCGGTGCC TGAACAAACA GCAGAAGCCT 
CTGGAAGAAT GTGAAGATGA TGAAGGGGTA CACACCGAGG AGCTGGACAG TGAACTAGAG 
CTGCCTGCTG GCTGGGAAAA GATTGAAGAC CCTGTCTACG GTGTCTACTA TGTAGACCAC 
ATCAACAGGA AGACGCAATA TGAAAACCCA GTCCTAGAAG CCAAAAGGAA GAGACAGCTT 
GAACAACAGC AGCAGCAGCA GCAGCACCAG CAGCAGCCGC AGCAGCCTCA GCCACCGCAG 
CCAGAAGAGT GGACAGAAGA TCATGCATCT GTTGTGCCTC CTGTTGCTCC TTCCCATCCC 
CCAAGCAACC CGGAGCCAGC CAGGGAAGCT CCACTTCAGG GCAAACCTTT TTTTACAAGA 
AACCCTTCTG AGCTGGAAGG CAAGTTCATT CACACAAAGC TACGGAAAAG CAGTCGAGGC 
TTTGGCTTCA CGGTGGTTGG AGGGGACGAA CCTGATGAGT TCCTGCAGAT CAAGAGCCTT 
GTCCTAGATG GTCCTGCTGC ACTGGATGGC AAGATGGAGA CAGGAGATGT AATCGTCAGT 
GTGAATGACA CCTGTGTTTT GGGACACACA CATGCTCAAG TTGTGAAAAT TTTCCAGTCC 
ATTCCCATTG GTGCCAGTGT GGACCTTGAA CTCTGCAGAG GTTATCCATT GCCTTTTGAC 
CCGGATGACC CTAACACAAG TTTAGTGACC TCGGTGGCCA TTTTGGACAA AGAACCAATA 
ATTGTAAACG GACAAGAGAC CTATGATTCA CCAGCAAGCC ACAGTAGTAA AACGGGCAAA 
GTCAGCAACA TGAAGGATGC CAGGCCAAGC AGCCCCGCAG ACGTGGCTTC CAACAGTTCT 
CATGGGTATC CCAACGACAC AGTCTCCTTG GCTTCCTCCA TAGCCACCCA GCCAGAGCTA 
ATAACCGTTC ACATAGTCAA AGGGCCAATG GGATTTGGCT TTACGATCGC AGATAGTCCC 
GGTGGGGGTG GCCAAAGAGT GAAGCAGATT GTTGACAGTC CGCGCTGCAG AGGCCTCAAA 
GAAGGGGATC TGATTGTGGA GGTGAATAAG AGGAACGTGC AGGCCCTGAC GCACAATCAA 
GTCGTGGATA TGCTGATTGA GTGTCCAAAG GGAAGTGAGG TCACACTGTT GGTGCAGCGA 
GGAGGGCTAC CAGTTCCCAA GAAGAGTCCA AAGTCGCAGC CACTGGAGAG GAAAGACAGC 
CAGAACAGCT CCCAGCACAG TGTGTCCAGC CTCCGGAGCC TGCACACAGC TTCCCCGAGC 
CACAGTGCAC AGGTGCTCCC TGAGTACCCA CCTGCAGACG TCCCTGCTCC AGATCAGACC 
GACAGCTCTG GGCAGAAAAA GCCAGATCCC TTTAAAATCT GGGCCCAGTC GAGGAGCATG 
TATGAAGACC GACCTATGTC ACCTTCGCCT GCGTCAGGAC TGAGCAAGGG TGAAAGAGAC 
AGAGAGATCA ATTCCACGAA TTTTGGAGAA TGTCAGATTC CAGATTACCA GGAGCAGGAC 
ATCTTCCTCT GGAGAAAAGA GACCGGTTTT GGATTTAGGA TTCTGGGTGG AAATGAGCCA 
GGGGAACCCA TTTATATCGG TCACATCGTA CCACTGGGTG CTGCTGACAC AGACGGCCGC 
CTGAGGTCTG GAGATGAATT AATCTGTGTG GATGGGACAC CAGTAATTGG GAAATCACAC 
CAGCTCGTGG TCCAGCTTAT GCAACAAGCT GCCAAGCAAG GCCACGTCAA TCTCACAGTG 
AGGCGTAAAG TGGTGTTTAC GGTCCCCAAA GCAGAGAACG AGGTGCCCTC ACCAGCCTCA 
TCGCACCACA GTAGCAACCA GCCAGCGTCC CTGACGGAAG AGAAGCGCAC ACCGCAGGGC 
AGCCAGAACT CGCTGAACAC CGTGAGCTCA GGCAGCGGCA GCACCAGCGG CATTGGCAGT 
GGTGGTGGCG GAGGCAGCGG CGTGGTGAGC GCTGTGCTGC AGCCATATGA TGTGGAGATT 
CGGCGTGGGG AGAACGAGGG CTTTGGGTTT GTCATCGTGT CCTCCGTGAG CAGGCCCGAA 
GCGGGCACCA CCTTCGCAGG CAATGCATGT GTGGCTATGC CTCACAAAAT AGGTCGGATT 
ATTGAGGGGA GCCCTGCTGA CCGCTGTGGC AAGCTGAAAG TAGGAGACCG GATCTTGGCG 
GTAAATGGAT GTTCCATCAC CAACAAATCC CATTCTGACA TTGTCAACCT AATCAAAGAA 
GCAGGCAACA CAGTGACTCT CCGCATCATC CCCGGGGATG AGTCCTCCAA TGCCACACTG 
CTGACTAATG CGGAGAAGAT TGCCACCATC ACCACCACTC ATGCCCCCTC ACAACAGGGG 
ACCCAGGAAA CAAGGACCAC CACCAAACCA AAGCCGGATT CTCAGTTTGA GTTCAAAGGA 
CCGCAGGCTA CACAGGAGCA AGATTTTTAC ACTGTGGAAT TGGAAAGAGG AGCCAAGGGA 
TTTGGCTTTA GTCTTCGAGG AGGGCGAGAG TATAACATGG ACCTTTATGT TCTGCGCTTA 
GCAGAGGATG GTCCTGCAGA AAGATGCGGA AAGATGAGGA TTGGCGATGA AATTCTAGAG 
ATCAATGGTG AGACCACCAA AAACATGAAA CACTCTCGGG CCATAGAACT GATCAAGAAT 
GGCGGCCGCA GAGTCCGTCT GTTTCTGAGG CGGGGAGACG GCTCGGTCCC AGAATATGGT 
GGGTCAAACT ATGAAAACAT CCCTTCCTTC CCTGGCATGA CTCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Biochemical and morphological characterization of MAGI-1 in neuronal tissue.
Journal of neuroscience research90(9)1776-81(2012 Sep)
Ito H,Morishita R,Sudo K,Nishimura YV,Inaguma Y,Iwamoto I,Nagata K

A genome-wide study reveals rare CNVs exclusive to extreme phenotypes of Alzheimer disease.
European journal of human genetics : EJHG20(6)613-7(2012 Jun)
Rovelet-Lecrux A,Legallic S,Wallon D,Flaman JM,Martinaud O,Bombois S,Rollin-Sillaire A,Michon A,Le Ber I,Pariente J,Puel M,Paquet C,Croisile B,Thomas-Ant?rion C,Vercelletto M,L?vy R,Fr?bourg T,Hannequin D,Campion D,

Regulation of glutamate transporter GLT-1 by MAGI-1.
Journal of neurochemistry117(5)833-40(2011 Jun)
Zou S,Pita-Almenar JD,Eskin A

Interaction of endothelial cell-selective adhesion molecule and MAGI-1 promotes mature cell-cell adhesion via activation of RhoA.
Genes to cells : devoted to molecular & cellular mechanisms15(4)385-96(2010 Apr)
Kimura R,Ishida T,Kuriyama M,Hirata K,Hayashi Y

MAGI-1a functions as a scaffolding protein for the distal renal tubular basolateral K+ channels.
The Journal of biological chemistry283(18)12241-7(2008 May)
Tanemoto M,Toyohara T,Abe T,Ito S

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Magi1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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