Rpf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rpf1
Entrez Gene ID	499725
Full Name	ribosome production factor 1 homolog
Synonyms	Bxdc5
General protein information	Preferred Names ribosome production factor 1 homolog Names ribosome production factor 1 RNA processing factor 1 brix domain containing 5 brix domain-containing protein 5 ribosome biogenesis protein RPF1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q44

mRNA and Protein(s)

mRNA	Protein	Name
NM_001100794.1	NP_001094264.1	ribosome production factor 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RPF1	XP_524755.2
Rattus norvegicus (Norway rat)	Rpf1	NP_001094264.1
Caenorhabditis elegans (roundworm)	F44G4.1	NP_495918.1
Arabidopsis thaliana (thale cress)	MEE49	NP_567213.1
Homo sapiens (human)	RPF1	NP_079341.2
Canis lupus familiaris (dog)	RPF1	XP_537096.1
Mus musculus (house mouse)	Rpf1	NP_081647.2
Gallus gallus (chicken)	RPF1	XP_422376.4
Saccharomyces cerevisiae (baker's yeast)	RPF1	NP_011956.1
Eremothecium gossypii	AGOS_AEL093C	NP_984768.1
Bos taurus (cattle)	RPF1	NP_001076886.1
Danio rerio (zebrafish)	rpf1	NP_001002160.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009968	XP_319105.2
Xenopus tropicalis (tropical clawed frog)	rpf1	NP_001165011.1
	RPF1	XP_001106723.1
Drosophila melanogaster (fruit fly)	CG6712	NP_477479.1
Kluyveromyces lactis	KLLA0F21670g	XP_456047.1
Schizosaccharomyces pombe (fission yeast)	SPAC4F8.04	NP_593868.1
Oryza sativa (rice)	Os06g0137500	NP_001056733.1

Gene Ontology
Bibliography

Related articles in PubMed

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Functional proteomic analysis of human nucleolus.
Scherl A, Cout? Y, D?on C, Call? A, Kindbeiter K, Sanchez JC, Greco A, Hochstrasser D, Diaz JJ
Molecular biology of the cell13(11)4100-9(2002 Nov)

The sigma(70)-like motif: a eukaryotic RNA binding domain unique to a superfamily of proteins required for ribosome biogenesis.
Wehner KA, Baserga SJ
Molecular cell9(2)329-39(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rpf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rpf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09292	NM_001100794.1 Latest version!	Rattus norvegicus ribosome production factor 1 homolog (Rpf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rpf1 ( NM_001100794.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001094264.1 Rattus norvegicus ribosome production factor 1 homolog (Rpf1), mRNA.

Price & Availability↑

CloneID	ORa09292
Clone ID Related Accession (Same CDS sequence)	NM_001100794.1
Accession Version	NM_001100794.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	ribosome production factor 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC166888.1, DY564389.1 and BI299235.1. On or before Nov 21, 2008 this sequence version replaced XM_001077236.1, XM_001077189.1, XM_575059.2, XM_001059406.1. ##Evidence-Data-START## Transcript exon combination :: BC166888.1, FQ220191.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGAAGG CCGGCGAGAA AAGTGGCGGT GGTGGCAAGC GAGGCTTGAA GAGGAAAGCG 
CCCGCTGAAG AGCCTCAAGA AACTGCGGTC GCGAGCGATG GGACGGCGGA GAGCGGGGTA 
CAGTCCGCGA AAGCGGCTGC CTTCCCCCCG GGCTTCAGTA TCTCGGAGAT TAAGAACAAA 
CAGCGGCGAC ACTTGATGTT CACGCGGTGG AAACAGCAGC AGCGGAAGGA AAAGTTGGCA 
GCTAAGAAAA AACTTAAAAG GGAGAGAGAG GCTCTTGGTG ATAAGGCTCC ACCGAAGCCT 
GTACCCAAGA CCATTGACAA CCAACGCGTG TACGATGAGA CCACAGTAGA CCCCAATGAT 
GAAGAGGTGG CTTATGATGA GGCTACAGAT GAATTTGCTT CTTACTTCAA CAGACAGACG 
TCTCCTAAGA TTCTTATCAC AACCTCAGAC AGACCTCATG GGAGAACAGT ACGACTCTGT 
GAGCAGCTCT CAACAGTTAT ACCGGATTCA CATGTTTATT ACAGAAGAGG ACTGGCTCTG 
AAAAAAATTA TCCCACAGTG CATTGCAAGA GATTTCACAG ACCTAATCGT TATTAATGAA 
GATCGTAAAA CACCAAATGG ACTTATTTTG AGTCATTTGC CAAATGGTCC AACTGCTCAT 
TTTAAAATGA GTAGTGTTCG TCTTCGTAAA GAAATTAAGC GACGAGGCAA AGACCCCACA 
GAACATGTAC CTGAGATAAT TCTAAACAAC TTTACCACAC GGCTAGGTCA CTCTATCGGA 
CGTATGTTTG CTTCTCTCTT TCCTCATAAC CCTCAATTTA TTGGAAGACA AGTTGCCACG 
TTCCACAATC AGCGGGATTA CATCTTCTTC AGGTTTCACA GATACATATT CAAGAGTGAA 
AAGAAGGTGG GAATTCAGGA ACTTGGACCA CGTTTTACCT TAAAATTACG ATCTCTGCAG 
AAGGGAACCT TTGATTCCAA GTATGGAGAG TATGAATGGG TCCATAAGCC ACGGGAAATG 
GATACAAGTA GAAGAAAATT CCATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001094264.1
CDS	30..1079
Translation	MAKAGEKSGGGGKRGLKRKAPAEEPQETAVASDGTAESGVQSAKAAAFPPGFSISEIKNKQRRHLMFTRWKQQQRKEKLAAKKKLKREREALGDKAPPKPVPKTIDNQRVYDETTVDPNDEEVAYDEATDEFASYFNRQTSPKILITTSDRPHGRTVRLCEQLSTVIPDSHVYYRRGLALKKIIPQCIARDFTDLIVINEDRKTPNGLILSHLPNGPTAHFKMSSVRLRKEIKRRGKDPTEHVPEIILNNFTTRLGHSIGRMFASLFPHNPQFIGRQVATFHNQRDYIFFRFHRYIFKSEKKVGIQELGPRFTLKLRSLQKGTFDSKYGEYEWVHKPREMDTSRRKFHL

Target ORF information:

RefSeq Version	NM_001100794.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ribosome production factor 1 homolog (Rpf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100794.1

ORF Insert Sequence:

ATGGCGAAGG CCGGCGAGAA AAGTGGCGGT GGTGGCAAGC GAGGCTTGAA GAGGAAAGCG 
CCCGCTGAAG AGCCTCAAGA AACTGCGGTC GCGAGCGATG GGACGGCGGA GAGCGGGGTA 
CAGTCCGCGA AAGCGGCTGC CTTCCCCCCG GGCTTCAGTA TCTCGGAGAT TAAGAACAAA 
CAGCGGCGAC ACTTGATGTT CACGCGGTGG AAACAGCAGC AGCGGAAGGA AAAGTTGGCA 
GCTAAGAAAA AACTTAAAAG GGAGAGAGAG GCTCTTGGTG ATAAGGCTCC ACCGAAGCCT 
GTACCCAAGA CCATTGACAA CCAACGCGTG TACGATGAGA CCACAGTAGA CCCCAATGAT 
GAAGAGGTGG CTTATGATGA GGCTACAGAT GAATTTGCTT CTTACTTCAA CAGACAGACG 
TCTCCTAAGA TTCTTATCAC AACCTCAGAC AGACCTCATG GGAGAACAGT ACGACTCTGT 
GAGCAGCTCT CAACAGTTAT ACCGGATTCA CATGTTTATT ACAGAAGAGG ACTGGCTCTG 
AAAAAAATTA TCCCACAGTG CATTGCAAGA GATTTCACAG ACCTAATCGT TATTAATGAA 
GATCGTAAAA CACCAAATGG ACTTATTTTG AGTCATTTGC CAAATGGTCC AACTGCTCAT 
TTTAAAATGA GTAGTGTTCG TCTTCGTAAA GAAATTAAGC GACGAGGCAA AGACCCCACA 
GAACATGTAC CTGAGATAAT TCTAAACAAC TTTACCACAC GGCTAGGTCA CTCTATCGGA 
CGTATGTTTG CTTCTCTCTT TCCTCATAAC CCTCAATTTA TTGGAAGACA AGTTGCCACG 
TTCCACAATC AGCGGGATTA CATCTTCTTC AGGTTTCACA GATACATATT CAAGAGTGAA 
AAGAAGGTGG GAATTCAGGA ACTTGGACCA CGTTTTACCT TAAAATTACG ATCTCTGCAG 
AAGGGAACCT TTGATTCCAA GTATGGAGAG TATGAATGGG TCCATAAGCC ACGGGAAATG 
GATACAAGTA GAAGAAAATT CCATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Functional proteomic analysis of human nucleolus.
Molecular biology of the cell13(11)4100-9(2002 Nov)
Scherl A,Cout? Y,D?on C,Call? A,Kindbeiter K,Sanchez JC,Greco A,Hochstrasser D,Diaz JJ

The sigma(70)-like motif: a eukaryotic RNA binding domain unique to a superfamily of proteins required for ribosome biogenesis.
Molecular cell9(2)329-39(2002 Feb)
Wehner KA,Baserga SJ