Naaa cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Naaa
Entrez Gene ID	497009
Full Name	N-acylethanolamine acid amidase
Synonyms	Asahl
General protein information	Preferred Names N-acylethanolamine acid amidase Names N-acylethanolamine-hydrolyzing acid amidase ASAH-like protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14p22

mRNA and Protein(s)

mRNA	Protein	Name
NM_001010967.2	NP_001010967.1	N-acylethanolamine-hydrolyzing acid amidase precursor

Pathways from BioSystems

Homologies

	NAAA	XP_001099747.1
Bos taurus (cattle)	NAAA	NP_001093839.1
Mus musculus (house mouse)	Naaa	NP_080248.2
Rattus norvegicus (Norway rat)	Naaa	NP_001010967.1
Canis lupus familiaris (dog)	NAAA	XP_003640161.1
Caenorhabditis elegans (roundworm)	Y55D5A.3	NP_497647.1
Homo sapiens (human)	NAAA	NP_055250.2
Pan troglodytes (chimpanzee)	NAAA	XP_001152628.2
Gallus gallus (chicken)	NAAA	XP_420596.2
Xenopus tropicalis (tropical clawed frog)	naaa	NP_001005714.1

Gene Ontology
Bibliography

Related articles in PubMed

The tumor suppressor hTid1 inhibits STAT5b activity via functional interaction.
Dhennin-Duthille I, Nyga R, Yahiaoui S, Gouilleux-Gruart V, R?gnier A, Lassoued K, Gouilleux F
The Journal of biological chemistry286(7)5034-42(2011 Feb)

Proteolytic activation and glycosylation of N-acylethanolamine-hydrolyzing acid amidase, a lysosomal enzyme involved in the endocannabinoid metabolism.
Zhao LY, Tsuboi K, Okamoto Y, Nagahata S, Ueda N
Biochimica et biophysica acta1771(11)1397-405(2007 Nov)

Predominant expression of lysosomal N-acylethanolamine-hydrolyzing acid amidase in macrophages revealed by immunochemical studies.
Tsuboi K, Zhao LY, Okamoto Y, Araki N, Ueno M, Sakamoto H, Ueda N
Biochimica et biophysica acta1771(5)623-32(2007 May)

Molecular characterization of N-acylethanolamine-hydrolyzing acid amidase, a novel member of the choloylglycine hydrolase family with structural and functional similarity to acid ceramidase.
Tsuboi K, Sun YX, Okamoto Y, Araki N, Tonai T, Ueda N
The Journal of biological chemistry280(12)11082-92(2005 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mRNA and protein levels and activity of NAAA in alveolar macrophages are much higher than those in the whole lung tissue.
Title: Predominant expression of lysosomal N-acylethanolamine-hydrolyzing acid amidase in macrophages revealed by immunochemical studies.

The following Naaa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Naaa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01397	NM_001010967.2 Latest version!	Rattus norvegicus N-acylethanolamine acid amidase (Naaa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Naaa ( NM_001010967.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001010967.1 Rattus norvegicus N-acylethanolamine acid amidase (Naaa), mRNA.

Price & Availability↑

CloneID	ORa01397
Clone ID Related Accession (Same CDS sequence)	NM_001010967.2
Accession Version	NM_001010967.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	N-acylethanolamine-hydrolyzing acid amidase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC105771.1. On Oct 16, 2005 this sequence version replaced NM_001010967.1. ##Evidence-Data-START## Transcript exon combination :: BC105771.1, AB162193.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGGACCC CAGCCATCCG GGCCGCGTGC CATGGGGCAC ACCTGGCGCT GGCTCTGTTG 
CTGCTGCTGT CGCTGTCGGA TCCCTGGCTG TGGGCGACCG CCCCTGGCAC TCCGCCTCTC 
TTCAACGTCA GTCTGGACGC GGCCCCGGAG CTGCGCTGGC TGCCGATGCT GCAGCACTAC 
GACCCAGACT TCGTGCGCGC CGCGGTGGCG CAGGTTATTG GAGACAGGGT TCCCCAGTGG 
ATACTAGAGA TGATCGGAGA AATCGTGCAG AAGGTGGAGA GCTTCCTGCC TCAGCCCTTC 
ACCTCCGAGA TCCGCGGCAT ATGTGACTAC CTCAACCTCA GCCTGGCAGA GGGTGTCTTG 
GTCAACTTGG CCTATGAGGC TTCCGCATTC TGCACCAGTA TTGTGGCCCA AGACTCCCAA 
GGCCGTATTT ACCATGGCCG GAACCTGGAC TATCCTTTTG GAAATGCCTT ACGAAAGCTG 
ACAGCAGACG TGCAGTTTGT AAAGAACGGG CAGATTGTAT TCACAGCGAC CACTTTTGTT 
GGCTATGTAG GACTGTGGAC AGGTCAAAGT CCCCACAAGT TTACAATTTC TGGTGATGAA 
CGAGACAAAG GCTGGTGGTG GGAGAACATG ATTGCTGCGC TTTCCTTGGG ACACTCGCCC 
ATCAGCTGGC TTATCCGCAA AACCCTGACT GAGTCCGAAG ACTTCGAAGC GGCCGTTTAC 
ACGCTGGCCA AGACTCCCCT CATTGCTGAT GTGTATTACA TCGTTGGGGG TACATCGCCC 
CAGGAGGGAG TAGTCATCAC CAGGGACAGA GGTGGCCCCG CGGACATTTG GCCTCTTGAC 
CCTCTGAATG GAGCGTGGTT CCGAGTCGAG ACCAATTATG ACCACTGGGA GCCTGTGCCC 
AAGAGGGATG ACCGAAGGAC ACCAGCCATC AAAGCCCTAA ATGCCACAGG GCAAGCTCAC 
CTCAGTCTGG AGACCCTCTT CCAGGTTTTA TCCGTGTTTC CTGTTTATAA CAACTACACA 
ATTTATACCA CAGTGATGAG CGCTGCTGAG CCCGACAAGT ACATGACCAT GATCAGAAAC 
CCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001010967.1
CDS	18..1106
Translation	MGTPAIRAACHGAHLALALLLLLSLSDPWLWATAPGTPPLFNVSLDAAPELRWLPMLQHYDPDFVRAAVAQVIGDRVPQWILEMIGEIVQKVESFLPQPFTSEIRGICDYLNLSLAEGVLVNLAYEASAFCTSIVAQDSQGRIYHGRNLDYPFGNALRKLTADVQFVKNGQIVFTATTFVGYVGLWTGQSPHKFTISGDERDKGWWWENMIAALSLGHSPISWLIRKTLTESEDFEAAVYTLAKTPLIADVYYIVGGTSPQEGVVITRDRGGPADIWPLDPLNGAWFRVETNYDHWEPVPKRDDRRTPAIKALNATGQAHLSLETLFQVLSVFPVYNNYTIYTTVMSAAEPDKYMTMIRNPS

Target ORF information:

RefSeq Version	NM_001010967.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus N-acylethanolamine acid amidase (Naaa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001010967.2

ORF Insert Sequence:

ATGGGGACCC CAGCCATCCG GGCCGCGTGC CATGGGGCAC ACCTGGCGCT GGCTCTGTTG 
CTGCTGCTGT CGCTGTCGGA TCCCTGGCTG TGGGCGACCG CCCCTGGCAC TCCGCCTCTC 
TTCAACGTCA GTCTGGACGC GGCCCCGGAG CTGCGCTGGC TGCCGATGCT GCAGCACTAC 
GACCCAGACT TCGTGCGCGC CGCGGTGGCG CAGGTTATTG GAGACAGGGT TCCCCAGTGG 
ATACTAGAGA TGATCGGAGA AATCGTGCAG AAGGTGGAGA GCTTCCTGCC TCAGCCCTTC 
ACCTCCGAGA TCCGCGGCAT ATGTGACTAC CTCAACCTCA GCCTGGCAGA GGGTGTCTTG 
GTCAACTTGG CCTATGAGGC TTCCGCATTC TGCACCAGTA TTGTGGCCCA AGACTCCCAA 
GGCCGTATTT ACCATGGCCG GAACCTGGAC TATCCTTTTG GAAATGCCTT ACGAAAGCTG 
ACAGCAGACG TGCAGTTTGT AAAGAACGGG CAGATTGTAT TCACAGCGAC CACTTTTGTT 
GGCTATGTAG GACTGTGGAC AGGTCAAAGT CCCCACAAGT TTACAATTTC TGGTGATGAA 
CGAGACAAAG GCTGGTGGTG GGAGAACATG ATTGCTGCGC TTTCCTTGGG ACACTCGCCC 
ATCAGCTGGC TTATCCGCAA AACCCTGACT GAGTCCGAAG ACTTCGAAGC GGCCGTTTAC 
ACGCTGGCCA AGACTCCCCT CATTGCTGAT GTGTATTACA TCGTTGGGGG TACATCGCCC 
CAGGAGGGAG TAGTCATCAC CAGGGACAGA GGTGGCCCCG CGGACATTTG GCCTCTTGAC 
CCTCTGAATG GAGCGTGGTT CCGAGTCGAG ACCAATTATG ACCACTGGGA GCCTGTGCCC 
AAGAGGGATG ACCGAAGGAC ACCAGCCATC AAAGCCCTAA ATGCCACAGG GCAAGCTCAC 
CTCAGTCTGG AGACCCTCTT CCAGGTTTTA TCCGTGTTTC CTGTTTATAA CAACTACACA 
ATTTATACCA CAGTGATGAG CGCTGCTGAG CCCGACAAGT ACATGACCAT GATCAGAAAC 
CCAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The tumor suppressor hTid1 inhibits STAT5b activity via functional interaction.
The Journal of biological chemistry286(7)5034-42(2011 Feb)
Dhennin-Duthille I,Nyga R,Yahiaoui S,Gouilleux-Gruart V,R?gnier A,Lassoued K,Gouilleux F

Proteolytic activation and glycosylation of N-acylethanolamine-hydrolyzing acid amidase, a lysosomal enzyme involved in the endocannabinoid metabolism.
Biochimica et biophysica acta1771(11)1397-405(2007 Nov)
Zhao LY,Tsuboi K,Okamoto Y,Nagahata S,Ueda N

Predominant expression of lysosomal N-acylethanolamine-hydrolyzing acid amidase in macrophages revealed by immunochemical studies.
Biochimica et biophysica acta1771(5)623-32(2007 May)
Tsuboi K,Zhao LY,Okamoto Y,Araki N,Ueno M,Sakamoto H,Ueda N

Molecular characterization of N-acylethanolamine-hydrolyzing acid amidase, a novel member of the choloylglycine hydrolase family with structural and functional similarity to acid ceramidase.
The Journal of biological chemistry280(12)11082-92(2005 Mar)
Tsuboi K,Sun YX,Okamoto Y,Araki N,Tonai T,Ueda N