Dnmt3a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dnmt3a
Entrez Gene ID	444984
Full Name	DNA methyltransferase 3 alpha
General protein information	Preferred Names DNA methyltransferase 3 alpha Names DNA (cytosine-5)-methyltransferase 3A DNA (cytosine-5-)-methyltransferase 3 alpha DNA methyltransferase 3A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q14
Summary	member of a family of DNA methyltransferases [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017594267.1	XP_017449756.1	DNA (cytosine-5)-methyltransferase 3A isoform X1
XM_017594268.1	XP_017449757.1	DNA (cytosine-5)-methyltransferase 3A isoform X1
NM_001003958.1	NP_001003958.1	DNA (cytosine-5)-methyltransferase 3A isoform 1
XM_017594266.1	XP_017449755.1	DNA (cytosine-5)-methyltransferase 3A isoform X1
NM_001003957.1	NP_001003957.1	DNA (cytosine-5)-methyltransferase 3A isoform 2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	DNMT3A	NP_001020003.1
Pan troglodytes (chimpanzee)	DNMT3A	XP_001148731.1
Bos taurus (cattle)	DNMT3A	NP_001193431.1
Homo sapiens (human)	DNMT3A	NP_783328.1
Canis lupus familiaris (dog)	LOC482996	XP_005630271.1
Mus musculus (house mouse)	Dnmt3a	NP_031898.1
Rattus norvegicus (Norway rat)	Dnmt3a	NP_001003958.1
Danio rerio (zebrafish)	dnmt3ab	NP_001018150.1
Xenopus tropicalis (tropical clawed frog)	LOC100496503	XP_004919668.1

Gene Ontology
Bibliography

Related articles in PubMed

Nerve injury-induced epigenetic silencing of opioid receptors controlled by DNMT3a in primary afferent neurons.
Sun L, Zhao JY, Gu X, Liang L, Wu S, Mo K, Feng J, Guo W, Zhang J, Bekker A, Zhao X, Nestler EJ, Tao YX
Pain158(6)1153-1165(2017 Jun)

DNA methyltransferase DNMT3a contributes to neuropathic pain by repressing Kcna2 in primary afferent neurons.
Zhao JY, Liang L, Gu X, Li Z, Wu S, Sun L, Atianjoh FE, Feng J, Mo K, Jia S, Lutz BM, Bekker A, Nestler EJ, Tao YX
Nature communications814712(2017 Mar)

[EXPRESS] DNMT3a contributes to the development and maintenance of bone cancer pain by silencing Kv1.2 expression in spinal cord dorsal horn.
Miao XR, Fan LC, Wu S, Mao Q, Li Z, Lutz B, Xu JT, Lu Z, Tao YX
Molecular pain131744806917740681(2017 Jan-Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A severely altered chromatin structure during retinal degeneration coincided with an increased expression of the DNMT isozyme DNMT3a.
Title: DNA methylation and differential gene regulation in photoreceptor cell death.

The authors developed a resistant hepatocyte rat model of liver carcinogenesis showing underexpression of DNMT3a and DNMT3b during the early stages.
Title: DNA methyltransferases 3a and 3b are differentially expressed in the early stages of a rat liver carcinogenesis model.

We have found that during acute inflammatory pain induced by the application of 10% mustard oil on the tongues of rats, levels of DNMT3a and 3b were elevated markedly
Title: Epigenetic regulation of acute inflammatory pain.

DNMT3A and RASSF1A may serve as a new mechanism for cardiac fibrosis.
Title: DNMT3A silencing RASSF1A promotes cardiac fibrosis through upregulation of ERK1/2.

Topiramate prevented ethanol-induced increase in Dnmt3a expression in rate prefrontal cortex.
Title: Effects of topiramate on ethanol-cocaine interactions and DNA methyltransferase gene expression in the rat prefrontal cortex.

The following Dnmt3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dnmt3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa09906	NM_001003958.1 Latest version!	Rattus norvegicus DNA methyltransferase 3 alpha (Dnmt3a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa42447	NM_001003957.1 Latest version!	Rattus norvegicus DNA methyltransferase 3 alpha (Dnmt3a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa09906	XM_017594267.1 Latest version!	Rattus norvegicus DNA methyltransferase 3 alpha (Dnmt3a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa09906	XM_017594268.1 Latest version!	Rattus norvegicus DNA methyltransferase 3 alpha (Dnmt3a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa09906	XM_017594266.1 Latest version!	Rattus norvegicus DNA methyltransferase 3 alpha (Dnmt3a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dnmt3a ( NM_001003958.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001003958.1 Rattus norvegicus DNA methyltransferase 3 alpha (Dnmt3a), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa09906
Clone ID Related Accession (Same CDS sequence)	XM_017594267.1 , XM_017594268.1 , NM_001003958.1 , XM_017594266.1
Accession Version	NM_001003958.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Rattus norvegicus(Norway rat)
Product	DNA (cytosine-5)-methyltransferase 3A isoform 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BN000395.1. On Apr 19, 2005 this sequence version replaced XM_233941.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BN000395.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCCCTCCA GCGGCCCCGG GGACACCAGC ATCTCCTCTC TGGAGCGGGA GGATGATCGA 
AAGGAAGGAG AGGAACAGGA GGAGAACCGT GGTAAGGAAG AGCGTCAGGA GCCCAGCGCC 
ACGGCCCGGA AAGTGGGGAG GCCTGGCCGG AAGCGCAAGC ACCCACCGGT GGAAAGCAGT 
GACACCCCCA AGGACCCAGC GGTGACCACC AAGTCTCAGC CCACAGCCCA GGACTCTGGG 
CCCTCAGATC TGCTACCCAA TGGAGACTTG GAGAAGCGGA GTGAACCCCA ACCTGAGGAG 
GGGAGCCCAG CTGCAGGGCA GAAGGGTGGG GCCCCAGCTG AAGGAGAGGG AACTGAGACC 
CCACCAGAAG CCTCCAGAGC AGTGGAGAAT GGCTGCTGCG TAACCAAGGA AGGCCGTGGA 
GCCTCTGCGG GAGAGGGCAA AGAACAGAAG CAGACCAACA TCGAATCCAT GAAAATGGAG 
GGCTCCCGGG GCCGACTGCG TGGTGGCTTG GGGTGGGAGT CCAGCCTCCG TCAGCGGCCC 
ATGCCAAGAC TCACCTTCCA GGCAGGGGAC CCCTACTACA TCAGCAAACG GAAGCGGGAT 
GAGTGGCTGG CACGTTGGAA AAGGGAGGCT GAGAAGAAAG CCAAGGTGAT TGCAGTAATG 
AATGCTGTGG AGGAAAGCCA GGCCTCTGGG GAGTCCCAGA AGGTGGAGGA GGCCAGCCCT 
CCTGCAGTGC AGCAGCCCAC GGACCCTGCA TCTCCCACTG TGGCCACCAC CCCTGAGCCA 
GTAGGGGCTG ATGCTGGGGA CAAGAATGCT ACCAAGGCAG CTGATGACGA GCCCGAGTAT 
GAGGATGGCC GGGGCTTTGG CATTGGAGAG CTGGTGTGGG GGAAACTCCG GGGCTTCTCC 
TGGTGGCCAG GCCGAATTGT GTCTTGGTGG ATGACAGGCC GGAGCCGAGC AGCCGAAGGC 
ACTCGCTGGG TCATGTGGTT CGGAGATGGC AAATTCTCAG TGGTGTGTGT CGAGAAGCTC 
ATGCCCCTGA GCTCCTTCTG CAGTGCGTTC CACCAGGCCA CCTACAACAA GCAGCCCATG 
TACCGCAAAG CCATCTACGA AGTCCTCCAG GTGGCCAGCA GCCGTGCAGG GAAGCTGTTC 
CCAGCATGTC ATGACAGCGA TGAAAGTGAC ACTGGCAAGG CTGTGGAGGT GCAGAACAAG 
CAGATGATTG AGTGGGCCCT TGGCGGCTTC CAGCCCTCTG GTCCCAAAGG CCTGGAGCCA 
CCAGAAGAGG AGAAGAATCC TTACAAGGAA GTTTACACCG ACATGTGGGT GGAGCCTGAG 
GCAGCTGCTT ATGCCCCACC CCCACCAGCC AAGAAACCCA GAAAGAGCAC AACAGAGAAA 
CCCAAGGTCA AGGAGATCAT TGATGAACGC ACAAGAGAAC GGCTCGTGTA TGAGGTGCGC 
CAGAAGTGCC GAAACATCGA GGACATTTGT ATCTCATGTG GGAGCCTCAA TGTTACCCTG 
GAGCACCCAC TCTTCATTGG TGGAATGTGC CAGAACTGTA AGAACTGCTT CTTGGAGTGT 
GCTTACCAAT ACGATGACGA TGGGTACCAG TCCTACTGTA CCATCTGCTG TGGGGGGCGC 
GAAGTGCTCA TGTGTGGGAA CAACAACTGC TGCAGGTGCT TTTGTGTGGA GTGTGTGGAT 
CTCTTGGTGG GGCCAGGGGC TGCCCAAGCA GCCATTAAGG AAGACCCCTG GAACTGCTAC 
ATGTGTGGGC ACAAGGGCAC GTATGGGCTG CTGCGGAGAC GGGAGGACTG GCCTTCTCGA 
CTCCAGATGT TCTTCGCCAA TAACCACGAC CAGGAATTTG ACCCCCCGAA GGTTTACCCA 
CCTGTGCCAG CTGAGAAGAG GAAGCCCATC CGGGTGCTAT CTCTCTTTGA TGGGATTGCT 
ACAGGGCTCC TGGTGCTGAA GGACCTGGGC ATCCAAGTGG ACCGCTACAT CGCCTCCGAG 
GTGTGCGAGG ACTCCATCAC AGTGGGCATG GTGCGGCACC AGGGAAAGAT CATGTACGTC 
GGGGACGTCC GCAGCGTCAC ACAGAAGCAT ATCCAGGAGT GGGGCCCATT CGATCTGGTG 
ATTGGGGGCA GTCCCTGCAA TGACCTCTCC ATCGTCAACC CTGCCCGCAA GGGACTTTAC 
GAGGGCACGG GCCGCCTCTT CTTTGAGTTC TACCGCCTCC TGCATGACGC GCGGCCCAAG 
GAGGGAGATG ATCGCCCCTT CTTCTGGCTT TTTGAGAATG TGGTGGCCAT GGGCGTTAGT 
GACAAGAGGG ACATCTCGAG ATTTCTTGAG TCTAACCCCG TGATGATCGA CGCCAAAGAA 
GTGTCTGCTG CACACAGGGC CCGTTATTTC TGGGGTAACC TTCCTGGCAT GAACAGGCCA 
TTGGCATCCA CTGTGAATGA TAAGCTGGAG TTGCAAGAGT GTCTGGAACA CGGCAGAATA 
GCCAAGTTCA GCAAAGTGAG GACCATTACC ACCAGGTCAA ACTCCATAAA GCAGGGCAAA 
GACCAGCATT TCCCTGTCTT CATGAATGAG AAGGAAGACA TCCTCTGGTG CACTGAAATG 
GAAAGGGTGT TTGGCTTCCC TGTCCACTAC ACAGACGTCT CCAACATGAG CCGCTTGGCG 
AGGCAGAGAC TGCTGGGCCG ATCGTGGAGC GTGCCAGTCA TCCGCCACCT CTTCGCTCCG 
CTGAAGGAAT ATTTTGCTTG TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003958.1
CDS	172..2898
Translation	MPSSGPGDTSISSLEREDDRKEGEEQEENRGKEERQEPSATARKVGRPGRKRKHPPVESSDTPKDPAVTTKSQPTAQDSGPSDLLPNGDLEKRSEPQPEEGSPAAGQKGGAPAEGEGTETPPEASRAVENGCCVTKEGRGASAGEGKEQKQTNIESMKMEGSRGRLRGGLGWESSLRQRPMPRLTFQAGDPYYISKRKRDEWLARWKREAEKKAKVIAVMNAVEESQASGESQKVEEASPPAVQQPTDPASPTVATTPEPVGADAGDKNATKAADDEPEYEDGRGFGIGELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDGKFSVVCVEKLMPLSSFCSAFHQATYNKQPMYRKAIYEVLQVASSRAGKLFPACHDSDESDTGKAVEVQNKQMIEWALGGFQPSGPKGLEPPEEEKNPYKEVYTDMWVEPEAAAYAPPPPAKKPRKSTTEKPKVKEIIDERTRERLVYEVRQKCRNIEDICISCGSLNVTLEHPLFIGGMCQNCKNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVDLLVGPGAAQAAIKEDPWNCYMCGHKGTYGLLRRREDWPSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKHIQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFENVVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV

Target ORF information:

RefSeq Version	NM_001003958.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DNA methyltransferase 3 alpha (Dnmt3a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003958.1

ORF Insert Sequence:

ATGCCCTCCA GCGGCCCCGG GGACACCAGC ATCTCCTCTC TGGAGCGGGA GGATGATCGA 
AAGGAAGGAG AGGAACAGGA GGAGAACCGT GGTAAGGAAG AGCGTCAGGA GCCCAGCGCC 
ACGGCCCGGA AAGTGGGGAG GCCTGGCCGG AAGCGCAAGC ACCCACCGGT GGAAAGCAGT 
GACACCCCCA AGGACCCAGC GGTGACCACC AAGTCTCAGC CCACAGCCCA GGACTCTGGG 
CCCTCAGATC TGCTACCCAA TGGAGACTTG GAGAAGCGGA GTGAACCCCA ACCTGAGGAG 
GGGAGCCCAG CTGCAGGGCA GAAGGGTGGG GCCCCAGCTG AAGGAGAGGG AACTGAGACC 
CCACCAGAAG CCTCCAGAGC AGTGGAGAAT GGCTGCTGCG TAACCAAGGA AGGCCGTGGA 
GCCTCTGCGG GAGAGGGCAA AGAACAGAAG CAGACCAACA TCGAATCCAT GAAAATGGAG 
GGCTCCCGGG GCCGACTGCG TGGTGGCTTG GGGTGGGAGT CCAGCCTCCG TCAGCGGCCC 
ATGCCAAGAC TCACCTTCCA GGCAGGGGAC CCCTACTACA TCAGCAAACG GAAGCGGGAT 
GAGTGGCTGG CACGTTGGAA AAGGGAGGCT GAGAAGAAAG CCAAGGTGAT TGCAGTAATG 
AATGCTGTGG AGGAAAGCCA GGCCTCTGGG GAGTCCCAGA AGGTGGAGGA GGCCAGCCCT 
CCTGCAGTGC AGCAGCCCAC GGACCCTGCA TCTCCCACTG TGGCCACCAC CCCTGAGCCA 
GTAGGGGCTG ATGCTGGGGA CAAGAATGCT ACCAAGGCAG CTGATGACGA GCCCGAGTAT 
GAGGATGGCC GGGGCTTTGG CATTGGAGAG CTGGTGTGGG GGAAACTCCG GGGCTTCTCC 
TGGTGGCCAG GCCGAATTGT GTCTTGGTGG ATGACAGGCC GGAGCCGAGC AGCCGAAGGC 
ACTCGCTGGG TCATGTGGTT CGGAGATGGC AAATTCTCAG TGGTGTGTGT CGAGAAGCTC 
ATGCCCCTGA GCTCCTTCTG CAGTGCGTTC CACCAGGCCA CCTACAACAA GCAGCCCATG 
TACCGCAAAG CCATCTACGA AGTCCTCCAG GTGGCCAGCA GCCGTGCAGG GAAGCTGTTC 
CCAGCATGTC ATGACAGCGA TGAAAGTGAC ACTGGCAAGG CTGTGGAGGT GCAGAACAAG 
CAGATGATTG AGTGGGCCCT TGGCGGCTTC CAGCCCTCTG GTCCCAAAGG CCTGGAGCCA 
CCAGAAGAGG AGAAGAATCC TTACAAGGAA GTTTACACCG ACATGTGGGT GGAGCCTGAG 
GCAGCTGCTT ATGCCCCACC CCCACCAGCC AAGAAACCCA GAAAGAGCAC AACAGAGAAA 
CCCAAGGTCA AGGAGATCAT TGATGAACGC ACAAGAGAAC GGCTCGTGTA TGAGGTGCGC 
CAGAAGTGCC GAAACATCGA GGACATTTGT ATCTCATGTG GGAGCCTCAA TGTTACCCTG 
GAGCACCCAC TCTTCATTGG TGGAATGTGC CAGAACTGTA AGAACTGCTT CTTGGAGTGT 
GCTTACCAAT ACGATGACGA TGGGTACCAG TCCTACTGTA CCATCTGCTG TGGGGGGCGC 
GAAGTGCTCA TGTGTGGGAA CAACAACTGC TGCAGGTGCT TTTGTGTGGA GTGTGTGGAT 
CTCTTGGTGG GGCCAGGGGC TGCCCAAGCA GCCATTAAGG AAGACCCCTG GAACTGCTAC 
ATGTGTGGGC ACAAGGGCAC GTATGGGCTG CTGCGGAGAC GGGAGGACTG GCCTTCTCGA 
CTCCAGATGT TCTTCGCCAA TAACCACGAC CAGGAATTTG ACCCCCCGAA GGTTTACCCA 
CCTGTGCCAG CTGAGAAGAG GAAGCCCATC CGGGTGCTAT CTCTCTTTGA TGGGATTGCT 
ACAGGGCTCC TGGTGCTGAA GGACCTGGGC ATCCAAGTGG ACCGCTACAT CGCCTCCGAG 
GTGTGCGAGG ACTCCATCAC AGTGGGCATG GTGCGGCACC AGGGAAAGAT CATGTACGTC 
GGGGACGTCC GCAGCGTCAC ACAGAAGCAT ATCCAGGAGT GGGGCCCATT CGATCTGGTG 
ATTGGGGGCA GTCCCTGCAA TGACCTCTCC ATCGTCAACC CTGCCCGCAA GGGACTTTAC 
GAGGGCACGG GCCGCCTCTT CTTTGAGTTC TACCGCCTCC TGCATGACGC GCGGCCCAAG 
GAGGGAGATG ATCGCCCCTT CTTCTGGCTT TTTGAGAATG TGGTGGCCAT GGGCGTTAGT 
GACAAGAGGG ACATCTCGAG ATTTCTTGAG TCTAACCCCG TGATGATCGA CGCCAAAGAA 
GTGTCTGCTG CACACAGGGC CCGTTATTTC TGGGGTAACC TTCCTGGCAT GAACAGGCCA 
TTGGCATCCA CTGTGAATGA TAAGCTGGAG TTGCAAGAGT GTCTGGAACA CGGCAGAATA 
GCCAAGTTCA GCAAAGTGAG GACCATTACC ACCAGGTCAA ACTCCATAAA GCAGGGCAAA 
GACCAGCATT TCCCTGTCTT CATGAATGAG AAGGAAGACA TCCTCTGGTG CACTGAAATG 
GAAAGGGTGT TTGGCTTCCC TGTCCACTAC ACAGACGTCT CCAACATGAG CCGCTTGGCG 
AGGCAGAGAC TGCTGGGCCG ATCGTGGAGC GTGCCAGTCA TCCGCCACCT CTTCGCTCCG 
CTGAAGGAAT ATTTTGCTTG TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dnmt3a ( NM_001003957.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001003957.1 Rattus norvegicus DNA methyltransferase 3 alpha (Dnmt3a), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa42447
Clone ID Related Accession (Same CDS sequence)	NM_001003957.1
Accession Version	NM_001003957.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	DNA (cytosine-5)-methyltransferase 3A isoform 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BN000396.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BN000396.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689601, SAMN00632250 [ECO:0000348] ##Evidence-Data-END##

ATGAATGCTG TGGAGGAAAG CCAGGCCTCT GGGGAGTCCC AGAAGGTGGA GGAGGCCAGC 
CCTCCTGCAG TGCAGCAGCC CACGGACCCT GCATCTCCCA CTGTGGCCAC CACCCCTGAG 
CCAGTAGGGG CTGATGCTGG GGACAAGAAT GCTACCAAGG CAGCTGATGA CGAGCCCGAG 
TATGAGGATG GCCGGGGCTT TGGCATTGGA GAGCTGGTGT GGGGGAAACT CCGGGGCTTC 
TCCTGGTGGC CAGGCCGAAT TGTGTCTTGG TGGATGACAG GCCGGAGCCG AGCAGCCGAA 
GGCACTCGCT GGGTCATGTG GTTCGGAGAT GGCAAATTCT CAGTGGTGTG TGTCGAGAAG 
CTCATGCCCC TGAGCTCCTT CTGCAGTGCG TTCCACCAGG CCACCTACAA CAAGCAGCCC 
ATGTACCGCA AAGCCATCTA CGAAGTCCTC CAGGTGGCCA GCAGCCGTGC AGGGAAGCTG 
TTCCCAGCAT GTCATGACAG CGATGAAAGT GACACTGGCA AGGCTGTGGA GGTGCAGAAC 
AAGCAGATGA TTGAGTGGGC CCTTGGCGGC TTCCAGCCCT CTGGTCCCAA AGGCCTGGAG 
CCACCAGAAG AGGAGAAGAA TCCTTACAAG GAAGTTTACA CCGACATGTG GGTGGAGCCT 
GAGGCAGCTG CTTATGCCCC ACCCCCACCA GCCAAGAAAC CCAGAAAGAG CACAACAGAG 
AAACCCAAGG TCAAGGAGAT CATTGATGAA CGCACAAGAG AACGGCTCGT GTATGAGGTG 
CGCCAGAAGT GCCGAAACAT CGAGGACATT TGTATCTCAT GTGGGAGCCT CAATGTTACC 
CTGGAGCACC CACTCTTCAT TGGTGGAATG TGCCAGAACT GTAAGAACTG CTTCTTGGAG 
TGTGCTTACC AATACGATGA CGATGGGTAC CAGTCCTACT GTACCATCTG CTGTGGGGGG 
CGCGAAGTGC TCATGTGTGG GAACAACAAC TGCTGCAGGT GCTTTTGTGT GGAGTGTGTG 
GATCTCTTGG TGGGGCCAGG GGCTGCCCAA GCAGCCATTA AGGAAGACCC CTGGAACTGC 
TACATGTGTG GGCACAAGGG CACGTATGGG CTGCTGCGGA GACGGGAGGA CTGGCCTTCT 
CGACTCCAGA TGTTCTTCGC CAATAACCAC GACCAGGAAT TTGACCCCCC GAAGGTTTAC 
CCACCTGTGC CAGCTGAGAA GAGGAAGCCC ATCCGGGTGC TATCTCTCTT TGATGGGATT 
GCTACAGGGC TCCTGGTGCT GAAGGACCTG GGCATCCAAG TGGACCGCTA CATCGCCTCC 
GAGGTGTGCG AGGACTCCAT CACAGTGGGC ATGGTGCGGC ACCAGGGAAA GATCATGTAC 
GTCGGGGACG TCCGCAGCGT CACACAGAAG CATATCCAGG AGTGGGGCCC ATTCGATCTG 
GTGATTGGGG GCAGTCCCTG CAATGACCTC TCCATCGTCA ACCCTGCCCG CAAGGGACTT 
TACGAGGGCA CGGGCCGCCT CTTCTTTGAG TTCTACCGCC TCCTGCATGA CGCGCGGCCC 
AAGGAGGGAG ATGATCGCCC CTTCTTCTGG CTTTTTGAGA ATGTGGTGGC CATGGGCGTT 
AGTGACAAGA GGGACATCTC GAGATTTCTT GAGTCTAACC CCGTGATGAT CGACGCCAAA 
GAAGTGTCTG CTGCACACAG GGCCCGTTAT TTCTGGGGTA ACCTTCCTGG CATGAACAGG 
CCATTGGCAT CCACTGTGAA TGATAAGCTG GAGTTGCAAG AGTGTCTGGA ACACGGCAGA 
ATAGCCAAGT TCAGCAAAGT GAGGACCATT ACCACCAGGT CAAACTCCAT AAAGCAGGGC 
AAAGACCAGC ATTTCCCTGT CTTCATGAAT GAGAAGGAAG ACATCCTCTG GTGCACTGAA 
ATGGAAAGGG TGTTTGGCTT CCCTGTCCAC TACACAGACG TCTCCAACAT GAGCCGCTTG 
GCGAGGCAGA GACTGCTGGG CCGATCGTGG AGCGTGCCAG TCATCCGCCA CCTCTTCGCT 
CCGCTGAAGG AATATTTTGC TTGTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003957.1
CDS	147..2216
Translation	MNAVEESQASGESQKVEEASPPAVQQPTDPASPTVATTPEPVGADAGDKNATKAADDEPEYEDGRGFGIGELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDGKFSVVCVEKLMPLSSFCSAFHQATYNKQPMYRKAIYEVLQVASSRAGKLFPACHDSDESDTGKAVEVQNKQMIEWALGGFQPSGPKGLEPPEEEKNPYKEVYTDMWVEPEAAAYAPPPPAKKPRKSTTEKPKVKEIIDERTRERLVYEVRQKCRNIEDICISCGSLNVTLEHPLFIGGMCQNCKNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVDLLVGPGAAQAAIKEDPWNCYMCGHKGTYGLLRRREDWPSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKHIQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFENVVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV

Target ORF information:

RefSeq Version	NM_001003957.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DNA methyltransferase 3 alpha (Dnmt3a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003957.1

ORF Insert Sequence:

ATGAATGCTG TGGAGGAAAG CCAGGCCTCT GGGGAGTCCC AGAAGGTGGA GGAGGCCAGC 
CCTCCTGCAG TGCAGCAGCC CACGGACCCT GCATCTCCCA CTGTGGCCAC CACCCCTGAG 
CCAGTAGGGG CTGATGCTGG GGACAAGAAT GCTACCAAGG CAGCTGATGA CGAGCCCGAG 
TATGAGGATG GCCGGGGCTT TGGCATTGGA GAGCTGGTGT GGGGGAAACT CCGGGGCTTC 
TCCTGGTGGC CAGGCCGAAT TGTGTCTTGG TGGATGACAG GCCGGAGCCG AGCAGCCGAA 
GGCACTCGCT GGGTCATGTG GTTCGGAGAT GGCAAATTCT CAGTGGTGTG TGTCGAGAAG 
CTCATGCCCC TGAGCTCCTT CTGCAGTGCG TTCCACCAGG CCACCTACAA CAAGCAGCCC 
ATGTACCGCA AAGCCATCTA CGAAGTCCTC CAGGTGGCCA GCAGCCGTGC AGGGAAGCTG 
TTCCCAGCAT GTCATGACAG CGATGAAAGT GACACTGGCA AGGCTGTGGA GGTGCAGAAC 
AAGCAGATGA TTGAGTGGGC CCTTGGCGGC TTCCAGCCCT CTGGTCCCAA AGGCCTGGAG 
CCACCAGAAG AGGAGAAGAA TCCTTACAAG GAAGTTTACA CCGACATGTG GGTGGAGCCT 
GAGGCAGCTG CTTATGCCCC ACCCCCACCA GCCAAGAAAC CCAGAAAGAG CACAACAGAG 
AAACCCAAGG TCAAGGAGAT CATTGATGAA CGCACAAGAG AACGGCTCGT GTATGAGGTG 
CGCCAGAAGT GCCGAAACAT CGAGGACATT TGTATCTCAT GTGGGAGCCT CAATGTTACC 
CTGGAGCACC CACTCTTCAT TGGTGGAATG TGCCAGAACT GTAAGAACTG CTTCTTGGAG 
TGTGCTTACC AATACGATGA CGATGGGTAC CAGTCCTACT GTACCATCTG CTGTGGGGGG 
CGCGAAGTGC TCATGTGTGG GAACAACAAC TGCTGCAGGT GCTTTTGTGT GGAGTGTGTG 
GATCTCTTGG TGGGGCCAGG GGCTGCCCAA GCAGCCATTA AGGAAGACCC CTGGAACTGC 
TACATGTGTG GGCACAAGGG CACGTATGGG CTGCTGCGGA GACGGGAGGA CTGGCCTTCT 
CGACTCCAGA TGTTCTTCGC CAATAACCAC GACCAGGAAT TTGACCCCCC GAAGGTTTAC 
CCACCTGTGC CAGCTGAGAA GAGGAAGCCC ATCCGGGTGC TATCTCTCTT TGATGGGATT 
GCTACAGGGC TCCTGGTGCT GAAGGACCTG GGCATCCAAG TGGACCGCTA CATCGCCTCC 
GAGGTGTGCG AGGACTCCAT CACAGTGGGC ATGGTGCGGC ACCAGGGAAA GATCATGTAC 
GTCGGGGACG TCCGCAGCGT CACACAGAAG CATATCCAGG AGTGGGGCCC ATTCGATCTG 
GTGATTGGGG GCAGTCCCTG CAATGACCTC TCCATCGTCA ACCCTGCCCG CAAGGGACTT 
TACGAGGGCA CGGGCCGCCT CTTCTTTGAG TTCTACCGCC TCCTGCATGA CGCGCGGCCC 
AAGGAGGGAG ATGATCGCCC CTTCTTCTGG CTTTTTGAGA ATGTGGTGGC CATGGGCGTT 
AGTGACAAGA GGGACATCTC GAGATTTCTT GAGTCTAACC CCGTGATGAT CGACGCCAAA 
GAAGTGTCTG CTGCACACAG GGCCCGTTAT TTCTGGGGTA ACCTTCCTGG CATGAACAGG 
CCATTGGCAT CCACTGTGAA TGATAAGCTG GAGTTGCAAG AGTGTCTGGA ACACGGCAGA 
ATAGCCAAGT TCAGCAAAGT GAGGACCATT ACCACCAGGT CAAACTCCAT AAAGCAGGGC 
AAAGACCAGC ATTTCCCTGT CTTCATGAAT GAGAAGGAAG ACATCCTCTG GTGCACTGAA 
ATGGAAAGGG TGTTTGGCTT CCCTGTCCAC TACACAGACG TCTCCAACAT GAGCCGCTTG 
GCGAGGCAGA GACTGCTGGG CCGATCGTGG AGCGTGCCAG TCATCCGCCA CCTCTTCGCT 
CCGCTGAAGG AATATTTTGC TTGTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dnmt3a ( XM_017594267.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449756.1 Rattus norvegicus DNA methyltransferase 3 alpha (Dnmt3a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa09906
Clone ID Related Accession (Same CDS sequence)	XM_017594267.1 , XM_017594268.1 , NM_001003958.1 , XM_017594266.1
Accession Version	XM_017594267.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	DNA (cytosine-5)-methyltransferase 3A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCCA GCGGCCCCGG GGACACCAGC ATCTCCTCTC TGGAGCGGGA GGATGATCGA 
AAGGAAGGAG AGGAACAGGA GGAGAACCGT GGTAAGGAAG AGCGTCAGGA GCCCAGCGCC 
ACGGCCCGGA AAGTGGGGAG GCCTGGCCGG AAGCGCAAGC ACCCACCGGT GGAAAGCAGT 
GACACCCCCA AGGACCCAGC GGTGACCACC AAGTCTCAGC CCACAGCCCA GGACTCTGGG 
CCCTCAGATC TGCTACCCAA TGGAGACTTG GAGAAGCGGA GTGAACCCCA ACCTGAGGAG 
GGGAGCCCAG CTGCAGGGCA GAAGGGTGGG GCCCCAGCTG AAGGAGAGGG AACTGAGACC 
CCACCAGAAG CCTCCAGAGC AGTGGAGAAT GGCTGCTGCG TAACCAAGGA AGGCCGTGGA 
GCCTCTGCGG GAGAGGGCAA AGAACAGAAG CAGACCAACA TCGAATCCAT GAAAATGGAG 
GGCTCCCGGG GCCGACTGCG TGGTGGCTTG GGGTGGGAGT CCAGCCTCCG TCAGCGGCCC 
ATGCCAAGAC TCACCTTCCA GGCAGGGGAC CCCTACTACA TCAGCAAACG GAAGCGGGAT 
GAGTGGCTGG CACGTTGGAA AAGGGAGGCT GAGAAGAAAG CCAAGGTGAT TGCAGTAATG 
AATGCTGTGG AGGAAAGCCA GGCCTCTGGG GAGTCCCAGA AGGTGGAGGA GGCCAGCCCT 
CCTGCAGTGC AGCAGCCCAC GGACCCTGCA TCTCCCACTG TGGCCACCAC CCCTGAGCCA 
GTAGGGGCTG ATGCTGGGGA CAAGAATGCT ACCAAGGCAG CTGATGACGA GCCCGAGTAT 
GAGGATGGCC GGGGCTTTGG CATTGGAGAG CTGGTGTGGG GGAAACTCCG GGGCTTCTCC 
TGGTGGCCAG GCCGAATTGT GTCTTGGTGG ATGACAGGCC GGAGCCGAGC AGCCGAAGGC 
ACTCGCTGGG TCATGTGGTT CGGAGATGGC AAATTCTCAG TGGTGTGTGT CGAGAAGCTC 
ATGCCCCTGA GCTCCTTCTG CAGTGCGTTC CACCAGGCCA CCTACAACAA GCAGCCCATG 
TACCGCAAAG CCATCTACGA AGTCCTCCAG GTGGCCAGCA GCCGTGCAGG GAAGCTGTTC 
CCAGCATGTC ATGACAGCGA TGAAAGTGAC ACTGGCAAGG CTGTGGAGGT GCAGAACAAG 
CAGATGATTG AGTGGGCCCT TGGCGGCTTC CAGCCCTCTG GTCCCAAAGG CCTGGAGCCA 
CCAGAAGAGG AGAAGAATCC TTACAAGGAA GTTTACACCG ACATGTGGGT GGAGCCTGAG 
GCAGCTGCTT ATGCCCCACC CCCACCAGCC AAGAAACCCA GAAAGAGCAC AACAGAGAAA 
CCCAAGGTCA AGGAGATCAT TGATGAACGC ACAAGAGAAC GGCTCGTGTA TGAGGTGCGC 
CAGAAGTGCC GAAACATCGA GGACATTTGT ATCTCATGTG GGAGCCTCAA TGTTACCCTG 
GAGCACCCAC TCTTCATTGG TGGAATGTGC CAGAACTGTA AGAACTGCTT CTTGGAGTGT 
GCTTACCAAT ACGATGACGA TGGGTACCAG TCCTACTGTA CCATCTGCTG TGGGGGGCGC 
GAAGTGCTCA TGTGTGGGAA CAACAACTGC TGCAGGTGCT TTTGTGTGGA GTGTGTGGAT 
CTCTTGGTGG GGCCAGGGGC TGCCCAAGCA GCCATTAAGG AAGACCCCTG GAACTGCTAC 
ATGTGTGGGC ACAAGGGCAC GTATGGGCTG CTGCGGAGAC GGGAGGACTG GCCTTCTCGA 
CTCCAGATGT TCTTCGCCAA TAACCACGAC CAGGAATTTG ACCCCCCGAA GGTTTACCCA 
CCTGTGCCAG CTGAGAAGAG GAAGCCCATC CGGGTGCTAT CTCTCTTTGA TGGGATTGCT 
ACAGGGCTCC TGGTGCTGAA GGACCTGGGC ATCCAAGTGG ACCGCTACAT CGCCTCCGAG 
GTGTGCGAGG ACTCCATCAC AGTGGGCATG GTGCGGCACC AGGGAAAGAT CATGTACGTC 
GGGGACGTCC GCAGCGTCAC ACAGAAGCAT ATCCAGGAGT GGGGCCCATT CGATCTGGTG 
ATTGGGGGCA GTCCCTGCAA TGACCTCTCC ATCGTCAACC CTGCCCGCAA GGGACTTTAC 
GAGGGCACGG GCCGCCTCTT CTTTGAGTTC TACCGCCTCC TGCATGACGC GCGGCCCAAG 
GAGGGAGATG ATCGCCCCTT CTTCTGGCTT TTTGAGAATG TGGTGGCCAT GGGCGTTAGT 
GACAAGAGGG ACATCTCGAG ATTTCTTGAG TCTAACCCCG TGATGATCGA CGCCAAAGAA 
GTGTCTGCTG CACACAGGGC CCGTTATTTC TGGGGTAACC TTCCTGGCAT GAACAGGCCA 
TTGGCATCCA CTGTGAATGA TAAGCTGGAG TTGCAAGAGT GTCTGGAACA CGGCAGAATA 
GCCAAGTTCA GCAAAGTGAG GACCATTACC ACCAGGTCAA ACTCCATAAA GCAGGGCAAA 
GACCAGCATT TCCCTGTCTT CATGAATGAG AAGGAAGACA TCCTCTGGTG CACTGAAATG 
GAAAGGGTGT TTGGCTTCCC TGTCCACTAC ACAGACGTCT CCAACATGAG CCGCTTGGCG 
AGGCAGAGAC TGCTGGGCCG ATCGTGGAGC GTGCCAGTCA TCCGCCACCT CTTCGCTCCG 
CTGAAGGAAT ATTTTGCTTG TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449756.1
CDS	242..2968
Translation	MPSSGPGDTSISSLEREDDRKEGEEQEENRGKEERQEPSATARKVGRPGRKRKHPPVESSDTPKDPAVTTKSQPTAQDSGPSDLLPNGDLEKRSEPQPEEGSPAAGQKGGAPAEGEGTETPPEASRAVENGCCVTKEGRGASAGEGKEQKQTNIESMKMEGSRGRLRGGLGWESSLRQRPMPRLTFQAGDPYYISKRKRDEWLARWKREAEKKAKVIAVMNAVEESQASGESQKVEEASPPAVQQPTDPASPTVATTPEPVGADAGDKNATKAADDEPEYEDGRGFGIGELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDGKFSVVCVEKLMPLSSFCSAFHQATYNKQPMYRKAIYEVLQVASSRAGKLFPACHDSDESDTGKAVEVQNKQMIEWALGGFQPSGPKGLEPPEEEKNPYKEVYTDMWVEPEAAAYAPPPPAKKPRKSTTEKPKVKEIIDERTRERLVYEVRQKCRNIEDICISCGSLNVTLEHPLFIGGMCQNCKNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVDLLVGPGAAQAAIKEDPWNCYMCGHKGTYGLLRRREDWPSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKHIQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFENVVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV

Target ORF information:

RefSeq Version	XM_017594267.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DNA methyltransferase 3 alpha (Dnmt3a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594267.1

ORF Insert Sequence:

ATGCCCTCCA GCGGCCCCGG GGACACCAGC ATCTCCTCTC TGGAGCGGGA GGATGATCGA 
AAGGAAGGAG AGGAACAGGA GGAGAACCGT GGTAAGGAAG AGCGTCAGGA GCCCAGCGCC 
ACGGCCCGGA AAGTGGGGAG GCCTGGCCGG AAGCGCAAGC ACCCACCGGT GGAAAGCAGT 
GACACCCCCA AGGACCCAGC GGTGACCACC AAGTCTCAGC CCACAGCCCA GGACTCTGGG 
CCCTCAGATC TGCTACCCAA TGGAGACTTG GAGAAGCGGA GTGAACCCCA ACCTGAGGAG 
GGGAGCCCAG CTGCAGGGCA GAAGGGTGGG GCCCCAGCTG AAGGAGAGGG AACTGAGACC 
CCACCAGAAG CCTCCAGAGC AGTGGAGAAT GGCTGCTGCG TAACCAAGGA AGGCCGTGGA 
GCCTCTGCGG GAGAGGGCAA AGAACAGAAG CAGACCAACA TCGAATCCAT GAAAATGGAG 
GGCTCCCGGG GCCGACTGCG TGGTGGCTTG GGGTGGGAGT CCAGCCTCCG TCAGCGGCCC 
ATGCCAAGAC TCACCTTCCA GGCAGGGGAC CCCTACTACA TCAGCAAACG GAAGCGGGAT 
GAGTGGCTGG CACGTTGGAA AAGGGAGGCT GAGAAGAAAG CCAAGGTGAT TGCAGTAATG 
AATGCTGTGG AGGAAAGCCA GGCCTCTGGG GAGTCCCAGA AGGTGGAGGA GGCCAGCCCT 
CCTGCAGTGC AGCAGCCCAC GGACCCTGCA TCTCCCACTG TGGCCACCAC CCCTGAGCCA 
GTAGGGGCTG ATGCTGGGGA CAAGAATGCT ACCAAGGCAG CTGATGACGA GCCCGAGTAT 
GAGGATGGCC GGGGCTTTGG CATTGGAGAG CTGGTGTGGG GGAAACTCCG GGGCTTCTCC 
TGGTGGCCAG GCCGAATTGT GTCTTGGTGG ATGACAGGCC GGAGCCGAGC AGCCGAAGGC 
ACTCGCTGGG TCATGTGGTT CGGAGATGGC AAATTCTCAG TGGTGTGTGT CGAGAAGCTC 
ATGCCCCTGA GCTCCTTCTG CAGTGCGTTC CACCAGGCCA CCTACAACAA GCAGCCCATG 
TACCGCAAAG CCATCTACGA AGTCCTCCAG GTGGCCAGCA GCCGTGCAGG GAAGCTGTTC 
CCAGCATGTC ATGACAGCGA TGAAAGTGAC ACTGGCAAGG CTGTGGAGGT GCAGAACAAG 
CAGATGATTG AGTGGGCCCT TGGCGGCTTC CAGCCCTCTG GTCCCAAAGG CCTGGAGCCA 
CCAGAAGAGG AGAAGAATCC TTACAAGGAA GTTTACACCG ACATGTGGGT GGAGCCTGAG 
GCAGCTGCTT ATGCCCCACC CCCACCAGCC AAGAAACCCA GAAAGAGCAC AACAGAGAAA 
CCCAAGGTCA AGGAGATCAT TGATGAACGC ACAAGAGAAC GGCTCGTGTA TGAGGTGCGC 
CAGAAGTGCC GAAACATCGA GGACATTTGT ATCTCATGTG GGAGCCTCAA TGTTACCCTG 
GAGCACCCAC TCTTCATTGG TGGAATGTGC CAGAACTGTA AGAACTGCTT CTTGGAGTGT 
GCTTACCAAT ACGATGACGA TGGGTACCAG TCCTACTGTA CCATCTGCTG TGGGGGGCGC 
GAAGTGCTCA TGTGTGGGAA CAACAACTGC TGCAGGTGCT TTTGTGTGGA GTGTGTGGAT 
CTCTTGGTGG GGCCAGGGGC TGCCCAAGCA GCCATTAAGG AAGACCCCTG GAACTGCTAC 
ATGTGTGGGC ACAAGGGCAC GTATGGGCTG CTGCGGAGAC GGGAGGACTG GCCTTCTCGA 
CTCCAGATGT TCTTCGCCAA TAACCACGAC CAGGAATTTG ACCCCCCGAA GGTTTACCCA 
CCTGTGCCAG CTGAGAAGAG GAAGCCCATC CGGGTGCTAT CTCTCTTTGA TGGGATTGCT 
ACAGGGCTCC TGGTGCTGAA GGACCTGGGC ATCCAAGTGG ACCGCTACAT CGCCTCCGAG 
GTGTGCGAGG ACTCCATCAC AGTGGGCATG GTGCGGCACC AGGGAAAGAT CATGTACGTC 
GGGGACGTCC GCAGCGTCAC ACAGAAGCAT ATCCAGGAGT GGGGCCCATT CGATCTGGTG 
ATTGGGGGCA GTCCCTGCAA TGACCTCTCC ATCGTCAACC CTGCCCGCAA GGGACTTTAC 
GAGGGCACGG GCCGCCTCTT CTTTGAGTTC TACCGCCTCC TGCATGACGC GCGGCCCAAG 
GAGGGAGATG ATCGCCCCTT CTTCTGGCTT TTTGAGAATG TGGTGGCCAT GGGCGTTAGT 
GACAAGAGGG ACATCTCGAG ATTTCTTGAG TCTAACCCCG TGATGATCGA CGCCAAAGAA 
GTGTCTGCTG CACACAGGGC CCGTTATTTC TGGGGTAACC TTCCTGGCAT GAACAGGCCA 
TTGGCATCCA CTGTGAATGA TAAGCTGGAG TTGCAAGAGT GTCTGGAACA CGGCAGAATA 
GCCAAGTTCA GCAAAGTGAG GACCATTACC ACCAGGTCAA ACTCCATAAA GCAGGGCAAA 
GACCAGCATT TCCCTGTCTT CATGAATGAG AAGGAAGACA TCCTCTGGTG CACTGAAATG 
GAAAGGGTGT TTGGCTTCCC TGTCCACTAC ACAGACGTCT CCAACATGAG CCGCTTGGCG 
AGGCAGAGAC TGCTGGGCCG ATCGTGGAGC GTGCCAGTCA TCCGCCACCT CTTCGCTCCG 
CTGAAGGAAT ATTTTGCTTG TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dnmt3a ( XM_017594268.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449757.1 Rattus norvegicus DNA methyltransferase 3 alpha (Dnmt3a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa09906
Clone ID Related Accession (Same CDS sequence)	XM_017594267.1 , XM_017594268.1 , NM_001003958.1 , XM_017594266.1
Accession Version	XM_017594268.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	DNA (cytosine-5)-methyltransferase 3A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCCA GCGGCCCCGG GGACACCAGC ATCTCCTCTC TGGAGCGGGA GGATGATCGA 
AAGGAAGGAG AGGAACAGGA GGAGAACCGT GGTAAGGAAG AGCGTCAGGA GCCCAGCGCC 
ACGGCCCGGA AAGTGGGGAG GCCTGGCCGG AAGCGCAAGC ACCCACCGGT GGAAAGCAGT 
GACACCCCCA AGGACCCAGC GGTGACCACC AAGTCTCAGC CCACAGCCCA GGACTCTGGG 
CCCTCAGATC TGCTACCCAA TGGAGACTTG GAGAAGCGGA GTGAACCCCA ACCTGAGGAG 
GGGAGCCCAG CTGCAGGGCA GAAGGGTGGG GCCCCAGCTG AAGGAGAGGG AACTGAGACC 
CCACCAGAAG CCTCCAGAGC AGTGGAGAAT GGCTGCTGCG TAACCAAGGA AGGCCGTGGA 
GCCTCTGCGG GAGAGGGCAA AGAACAGAAG CAGACCAACA TCGAATCCAT GAAAATGGAG 
GGCTCCCGGG GCCGACTGCG TGGTGGCTTG GGGTGGGAGT CCAGCCTCCG TCAGCGGCCC 
ATGCCAAGAC TCACCTTCCA GGCAGGGGAC CCCTACTACA TCAGCAAACG GAAGCGGGAT 
GAGTGGCTGG CACGTTGGAA AAGGGAGGCT GAGAAGAAAG CCAAGGTGAT TGCAGTAATG 
AATGCTGTGG AGGAAAGCCA GGCCTCTGGG GAGTCCCAGA AGGTGGAGGA GGCCAGCCCT 
CCTGCAGTGC AGCAGCCCAC GGACCCTGCA TCTCCCACTG TGGCCACCAC CCCTGAGCCA 
GTAGGGGCTG ATGCTGGGGA CAAGAATGCT ACCAAGGCAG CTGATGACGA GCCCGAGTAT 
GAGGATGGCC GGGGCTTTGG CATTGGAGAG CTGGTGTGGG GGAAACTCCG GGGCTTCTCC 
TGGTGGCCAG GCCGAATTGT GTCTTGGTGG ATGACAGGCC GGAGCCGAGC AGCCGAAGGC 
ACTCGCTGGG TCATGTGGTT CGGAGATGGC AAATTCTCAG TGGTGTGTGT CGAGAAGCTC 
ATGCCCCTGA GCTCCTTCTG CAGTGCGTTC CACCAGGCCA CCTACAACAA GCAGCCCATG 
TACCGCAAAG CCATCTACGA AGTCCTCCAG GTGGCCAGCA GCCGTGCAGG GAAGCTGTTC 
CCAGCATGTC ATGACAGCGA TGAAAGTGAC ACTGGCAAGG CTGTGGAGGT GCAGAACAAG 
CAGATGATTG AGTGGGCCCT TGGCGGCTTC CAGCCCTCTG GTCCCAAAGG CCTGGAGCCA 
CCAGAAGAGG AGAAGAATCC TTACAAGGAA GTTTACACCG ACATGTGGGT GGAGCCTGAG 
GCAGCTGCTT ATGCCCCACC CCCACCAGCC AAGAAACCCA GAAAGAGCAC AACAGAGAAA 
CCCAAGGTCA AGGAGATCAT TGATGAACGC ACAAGAGAAC GGCTCGTGTA TGAGGTGCGC 
CAGAAGTGCC GAAACATCGA GGACATTTGT ATCTCATGTG GGAGCCTCAA TGTTACCCTG 
GAGCACCCAC TCTTCATTGG TGGAATGTGC CAGAACTGTA AGAACTGCTT CTTGGAGTGT 
GCTTACCAAT ACGATGACGA TGGGTACCAG TCCTACTGTA CCATCTGCTG TGGGGGGCGC 
GAAGTGCTCA TGTGTGGGAA CAACAACTGC TGCAGGTGCT TTTGTGTGGA GTGTGTGGAT 
CTCTTGGTGG GGCCAGGGGC TGCCCAAGCA GCCATTAAGG AAGACCCCTG GAACTGCTAC 
ATGTGTGGGC ACAAGGGCAC GTATGGGCTG CTGCGGAGAC GGGAGGACTG GCCTTCTCGA 
CTCCAGATGT TCTTCGCCAA TAACCACGAC CAGGAATTTG ACCCCCCGAA GGTTTACCCA 
CCTGTGCCAG CTGAGAAGAG GAAGCCCATC CGGGTGCTAT CTCTCTTTGA TGGGATTGCT 
ACAGGGCTCC TGGTGCTGAA GGACCTGGGC ATCCAAGTGG ACCGCTACAT CGCCTCCGAG 
GTGTGCGAGG ACTCCATCAC AGTGGGCATG GTGCGGCACC AGGGAAAGAT CATGTACGTC 
GGGGACGTCC GCAGCGTCAC ACAGAAGCAT ATCCAGGAGT GGGGCCCATT CGATCTGGTG 
ATTGGGGGCA GTCCCTGCAA TGACCTCTCC ATCGTCAACC CTGCCCGCAA GGGACTTTAC 
GAGGGCACGG GCCGCCTCTT CTTTGAGTTC TACCGCCTCC TGCATGACGC GCGGCCCAAG 
GAGGGAGATG ATCGCCCCTT CTTCTGGCTT TTTGAGAATG TGGTGGCCAT GGGCGTTAGT 
GACAAGAGGG ACATCTCGAG ATTTCTTGAG TCTAACCCCG TGATGATCGA CGCCAAAGAA 
GTGTCTGCTG CACACAGGGC CCGTTATTTC TGGGGTAACC TTCCTGGCAT GAACAGGCCA 
TTGGCATCCA CTGTGAATGA TAAGCTGGAG TTGCAAGAGT GTCTGGAACA CGGCAGAATA 
GCCAAGTTCA GCAAAGTGAG GACCATTACC ACCAGGTCAA ACTCCATAAA GCAGGGCAAA 
GACCAGCATT TCCCTGTCTT CATGAATGAG AAGGAAGACA TCCTCTGGTG CACTGAAATG 
GAAAGGGTGT TTGGCTTCCC TGTCCACTAC ACAGACGTCT CCAACATGAG CCGCTTGGCG 
AGGCAGAGAC TGCTGGGCCG ATCGTGGAGC GTGCCAGTCA TCCGCCACCT CTTCGCTCCG 
CTGAAGGAAT ATTTTGCTTG TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449757.1
CDS	280..3006
Translation	MPSSGPGDTSISSLEREDDRKEGEEQEENRGKEERQEPSATARKVGRPGRKRKHPPVESSDTPKDPAVTTKSQPTAQDSGPSDLLPNGDLEKRSEPQPEEGSPAAGQKGGAPAEGEGTETPPEASRAVENGCCVTKEGRGASAGEGKEQKQTNIESMKMEGSRGRLRGGLGWESSLRQRPMPRLTFQAGDPYYISKRKRDEWLARWKREAEKKAKVIAVMNAVEESQASGESQKVEEASPPAVQQPTDPASPTVATTPEPVGADAGDKNATKAADDEPEYEDGRGFGIGELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDGKFSVVCVEKLMPLSSFCSAFHQATYNKQPMYRKAIYEVLQVASSRAGKLFPACHDSDESDTGKAVEVQNKQMIEWALGGFQPSGPKGLEPPEEEKNPYKEVYTDMWVEPEAAAYAPPPPAKKPRKSTTEKPKVKEIIDERTRERLVYEVRQKCRNIEDICISCGSLNVTLEHPLFIGGMCQNCKNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVDLLVGPGAAQAAIKEDPWNCYMCGHKGTYGLLRRREDWPSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKHIQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFENVVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV

Target ORF information:

RefSeq Version	XM_017594268.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DNA methyltransferase 3 alpha (Dnmt3a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594268.1

ORF Insert Sequence:

ATGCCCTCCA GCGGCCCCGG GGACACCAGC ATCTCCTCTC TGGAGCGGGA GGATGATCGA 
AAGGAAGGAG AGGAACAGGA GGAGAACCGT GGTAAGGAAG AGCGTCAGGA GCCCAGCGCC 
ACGGCCCGGA AAGTGGGGAG GCCTGGCCGG AAGCGCAAGC ACCCACCGGT GGAAAGCAGT 
GACACCCCCA AGGACCCAGC GGTGACCACC AAGTCTCAGC CCACAGCCCA GGACTCTGGG 
CCCTCAGATC TGCTACCCAA TGGAGACTTG GAGAAGCGGA GTGAACCCCA ACCTGAGGAG 
GGGAGCCCAG CTGCAGGGCA GAAGGGTGGG GCCCCAGCTG AAGGAGAGGG AACTGAGACC 
CCACCAGAAG CCTCCAGAGC AGTGGAGAAT GGCTGCTGCG TAACCAAGGA AGGCCGTGGA 
GCCTCTGCGG GAGAGGGCAA AGAACAGAAG CAGACCAACA TCGAATCCAT GAAAATGGAG 
GGCTCCCGGG GCCGACTGCG TGGTGGCTTG GGGTGGGAGT CCAGCCTCCG TCAGCGGCCC 
ATGCCAAGAC TCACCTTCCA GGCAGGGGAC CCCTACTACA TCAGCAAACG GAAGCGGGAT 
GAGTGGCTGG CACGTTGGAA AAGGGAGGCT GAGAAGAAAG CCAAGGTGAT TGCAGTAATG 
AATGCTGTGG AGGAAAGCCA GGCCTCTGGG GAGTCCCAGA AGGTGGAGGA GGCCAGCCCT 
CCTGCAGTGC AGCAGCCCAC GGACCCTGCA TCTCCCACTG TGGCCACCAC CCCTGAGCCA 
GTAGGGGCTG ATGCTGGGGA CAAGAATGCT ACCAAGGCAG CTGATGACGA GCCCGAGTAT 
GAGGATGGCC GGGGCTTTGG CATTGGAGAG CTGGTGTGGG GGAAACTCCG GGGCTTCTCC 
TGGTGGCCAG GCCGAATTGT GTCTTGGTGG ATGACAGGCC GGAGCCGAGC AGCCGAAGGC 
ACTCGCTGGG TCATGTGGTT CGGAGATGGC AAATTCTCAG TGGTGTGTGT CGAGAAGCTC 
ATGCCCCTGA GCTCCTTCTG CAGTGCGTTC CACCAGGCCA CCTACAACAA GCAGCCCATG 
TACCGCAAAG CCATCTACGA AGTCCTCCAG GTGGCCAGCA GCCGTGCAGG GAAGCTGTTC 
CCAGCATGTC ATGACAGCGA TGAAAGTGAC ACTGGCAAGG CTGTGGAGGT GCAGAACAAG 
CAGATGATTG AGTGGGCCCT TGGCGGCTTC CAGCCCTCTG GTCCCAAAGG CCTGGAGCCA 
CCAGAAGAGG AGAAGAATCC TTACAAGGAA GTTTACACCG ACATGTGGGT GGAGCCTGAG 
GCAGCTGCTT ATGCCCCACC CCCACCAGCC AAGAAACCCA GAAAGAGCAC AACAGAGAAA 
CCCAAGGTCA AGGAGATCAT TGATGAACGC ACAAGAGAAC GGCTCGTGTA TGAGGTGCGC 
CAGAAGTGCC GAAACATCGA GGACATTTGT ATCTCATGTG GGAGCCTCAA TGTTACCCTG 
GAGCACCCAC TCTTCATTGG TGGAATGTGC CAGAACTGTA AGAACTGCTT CTTGGAGTGT 
GCTTACCAAT ACGATGACGA TGGGTACCAG TCCTACTGTA CCATCTGCTG TGGGGGGCGC 
GAAGTGCTCA TGTGTGGGAA CAACAACTGC TGCAGGTGCT TTTGTGTGGA GTGTGTGGAT 
CTCTTGGTGG GGCCAGGGGC TGCCCAAGCA GCCATTAAGG AAGACCCCTG GAACTGCTAC 
ATGTGTGGGC ACAAGGGCAC GTATGGGCTG CTGCGGAGAC GGGAGGACTG GCCTTCTCGA 
CTCCAGATGT TCTTCGCCAA TAACCACGAC CAGGAATTTG ACCCCCCGAA GGTTTACCCA 
CCTGTGCCAG CTGAGAAGAG GAAGCCCATC CGGGTGCTAT CTCTCTTTGA TGGGATTGCT 
ACAGGGCTCC TGGTGCTGAA GGACCTGGGC ATCCAAGTGG ACCGCTACAT CGCCTCCGAG 
GTGTGCGAGG ACTCCATCAC AGTGGGCATG GTGCGGCACC AGGGAAAGAT CATGTACGTC 
GGGGACGTCC GCAGCGTCAC ACAGAAGCAT ATCCAGGAGT GGGGCCCATT CGATCTGGTG 
ATTGGGGGCA GTCCCTGCAA TGACCTCTCC ATCGTCAACC CTGCCCGCAA GGGACTTTAC 
GAGGGCACGG GCCGCCTCTT CTTTGAGTTC TACCGCCTCC TGCATGACGC GCGGCCCAAG 
GAGGGAGATG ATCGCCCCTT CTTCTGGCTT TTTGAGAATG TGGTGGCCAT GGGCGTTAGT 
GACAAGAGGG ACATCTCGAG ATTTCTTGAG TCTAACCCCG TGATGATCGA CGCCAAAGAA 
GTGTCTGCTG CACACAGGGC CCGTTATTTC TGGGGTAACC TTCCTGGCAT GAACAGGCCA 
TTGGCATCCA CTGTGAATGA TAAGCTGGAG TTGCAAGAGT GTCTGGAACA CGGCAGAATA 
GCCAAGTTCA GCAAAGTGAG GACCATTACC ACCAGGTCAA ACTCCATAAA GCAGGGCAAA 
GACCAGCATT TCCCTGTCTT CATGAATGAG AAGGAAGACA TCCTCTGGTG CACTGAAATG 
GAAAGGGTGT TTGGCTTCCC TGTCCACTAC ACAGACGTCT CCAACATGAG CCGCTTGGCG 
AGGCAGAGAC TGCTGGGCCG ATCGTGGAGC GTGCCAGTCA TCCGCCACCT CTTCGCTCCG 
CTGAAGGAAT ATTTTGCTTG TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dnmt3a ( XM_017594266.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449755.1 Rattus norvegicus DNA methyltransferase 3 alpha (Dnmt3a), transcript variant X1, mRNA.

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CloneID	ORa09906
Clone ID Related Accession (Same CDS sequence)	XM_017594267.1 , XM_017594268.1 , NM_001003958.1 , XM_017594266.1
Accession Version	XM_017594266.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	DNA (cytosine-5)-methyltransferase 3A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCCA GCGGCCCCGG GGACACCAGC ATCTCCTCTC TGGAGCGGGA GGATGATCGA 
AAGGAAGGAG AGGAACAGGA GGAGAACCGT GGTAAGGAAG AGCGTCAGGA GCCCAGCGCC 
ACGGCCCGGA AAGTGGGGAG GCCTGGCCGG AAGCGCAAGC ACCCACCGGT GGAAAGCAGT 
GACACCCCCA AGGACCCAGC GGTGACCACC AAGTCTCAGC CCACAGCCCA GGACTCTGGG 
CCCTCAGATC TGCTACCCAA TGGAGACTTG GAGAAGCGGA GTGAACCCCA ACCTGAGGAG 
GGGAGCCCAG CTGCAGGGCA GAAGGGTGGG GCCCCAGCTG AAGGAGAGGG AACTGAGACC 
CCACCAGAAG CCTCCAGAGC AGTGGAGAAT GGCTGCTGCG TAACCAAGGA AGGCCGTGGA 
GCCTCTGCGG GAGAGGGCAA AGAACAGAAG CAGACCAACA TCGAATCCAT GAAAATGGAG 
GGCTCCCGGG GCCGACTGCG TGGTGGCTTG GGGTGGGAGT CCAGCCTCCG TCAGCGGCCC 
ATGCCAAGAC TCACCTTCCA GGCAGGGGAC CCCTACTACA TCAGCAAACG GAAGCGGGAT 
GAGTGGCTGG CACGTTGGAA AAGGGAGGCT GAGAAGAAAG CCAAGGTGAT TGCAGTAATG 
AATGCTGTGG AGGAAAGCCA GGCCTCTGGG GAGTCCCAGA AGGTGGAGGA GGCCAGCCCT 
CCTGCAGTGC AGCAGCCCAC GGACCCTGCA TCTCCCACTG TGGCCACCAC CCCTGAGCCA 
GTAGGGGCTG ATGCTGGGGA CAAGAATGCT ACCAAGGCAG CTGATGACGA GCCCGAGTAT 
GAGGATGGCC GGGGCTTTGG CATTGGAGAG CTGGTGTGGG GGAAACTCCG GGGCTTCTCC 
TGGTGGCCAG GCCGAATTGT GTCTTGGTGG ATGACAGGCC GGAGCCGAGC AGCCGAAGGC 
ACTCGCTGGG TCATGTGGTT CGGAGATGGC AAATTCTCAG TGGTGTGTGT CGAGAAGCTC 
ATGCCCCTGA GCTCCTTCTG CAGTGCGTTC CACCAGGCCA CCTACAACAA GCAGCCCATG 
TACCGCAAAG CCATCTACGA AGTCCTCCAG GTGGCCAGCA GCCGTGCAGG GAAGCTGTTC 
CCAGCATGTC ATGACAGCGA TGAAAGTGAC ACTGGCAAGG CTGTGGAGGT GCAGAACAAG 
CAGATGATTG AGTGGGCCCT TGGCGGCTTC CAGCCCTCTG GTCCCAAAGG CCTGGAGCCA 
CCAGAAGAGG AGAAGAATCC TTACAAGGAA GTTTACACCG ACATGTGGGT GGAGCCTGAG 
GCAGCTGCTT ATGCCCCACC CCCACCAGCC AAGAAACCCA GAAAGAGCAC AACAGAGAAA 
CCCAAGGTCA AGGAGATCAT TGATGAACGC ACAAGAGAAC GGCTCGTGTA TGAGGTGCGC 
CAGAAGTGCC GAAACATCGA GGACATTTGT ATCTCATGTG GGAGCCTCAA TGTTACCCTG 
GAGCACCCAC TCTTCATTGG TGGAATGTGC CAGAACTGTA AGAACTGCTT CTTGGAGTGT 
GCTTACCAAT ACGATGACGA TGGGTACCAG TCCTACTGTA CCATCTGCTG TGGGGGGCGC 
GAAGTGCTCA TGTGTGGGAA CAACAACTGC TGCAGGTGCT TTTGTGTGGA GTGTGTGGAT 
CTCTTGGTGG GGCCAGGGGC TGCCCAAGCA GCCATTAAGG AAGACCCCTG GAACTGCTAC 
ATGTGTGGGC ACAAGGGCAC GTATGGGCTG CTGCGGAGAC GGGAGGACTG GCCTTCTCGA 
CTCCAGATGT TCTTCGCCAA TAACCACGAC CAGGAATTTG ACCCCCCGAA GGTTTACCCA 
CCTGTGCCAG CTGAGAAGAG GAAGCCCATC CGGGTGCTAT CTCTCTTTGA TGGGATTGCT 
ACAGGGCTCC TGGTGCTGAA GGACCTGGGC ATCCAAGTGG ACCGCTACAT CGCCTCCGAG 
GTGTGCGAGG ACTCCATCAC AGTGGGCATG GTGCGGCACC AGGGAAAGAT CATGTACGTC 
GGGGACGTCC GCAGCGTCAC ACAGAAGCAT ATCCAGGAGT GGGGCCCATT CGATCTGGTG 
ATTGGGGGCA GTCCCTGCAA TGACCTCTCC ATCGTCAACC CTGCCCGCAA GGGACTTTAC 
GAGGGCACGG GCCGCCTCTT CTTTGAGTTC TACCGCCTCC TGCATGACGC GCGGCCCAAG 
GAGGGAGATG ATCGCCCCTT CTTCTGGCTT TTTGAGAATG TGGTGGCCAT GGGCGTTAGT 
GACAAGAGGG ACATCTCGAG ATTTCTTGAG TCTAACCCCG TGATGATCGA CGCCAAAGAA 
GTGTCTGCTG CACACAGGGC CCGTTATTTC TGGGGTAACC TTCCTGGCAT GAACAGGCCA 
TTGGCATCCA CTGTGAATGA TAAGCTGGAG TTGCAAGAGT GTCTGGAACA CGGCAGAATA 
GCCAAGTTCA GCAAAGTGAG GACCATTACC ACCAGGTCAA ACTCCATAAA GCAGGGCAAA 
GACCAGCATT TCCCTGTCTT CATGAATGAG AAGGAAGACA TCCTCTGGTG CACTGAAATG 
GAAAGGGTGT TTGGCTTCCC TGTCCACTAC ACAGACGTCT CCAACATGAG CCGCTTGGCG 
AGGCAGAGAC TGCTGGGCCG ATCGTGGAGC GTGCCAGTCA TCCGCCACCT CTTCGCTCCG 
CTGAAGGAAT ATTTTGCTTG TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449755.1
CDS	2526..5252
Translation	MPSSGPGDTSISSLEREDDRKEGEEQEENRGKEERQEPSATARKVGRPGRKRKHPPVESSDTPKDPAVTTKSQPTAQDSGPSDLLPNGDLEKRSEPQPEEGSPAAGQKGGAPAEGEGTETPPEASRAVENGCCVTKEGRGASAGEGKEQKQTNIESMKMEGSRGRLRGGLGWESSLRQRPMPRLTFQAGDPYYISKRKRDEWLARWKREAEKKAKVIAVMNAVEESQASGESQKVEEASPPAVQQPTDPASPTVATTPEPVGADAGDKNATKAADDEPEYEDGRGFGIGELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDGKFSVVCVEKLMPLSSFCSAFHQATYNKQPMYRKAIYEVLQVASSRAGKLFPACHDSDESDTGKAVEVQNKQMIEWALGGFQPSGPKGLEPPEEEKNPYKEVYTDMWVEPEAAAYAPPPPAKKPRKSTTEKPKVKEIIDERTRERLVYEVRQKCRNIEDICISCGSLNVTLEHPLFIGGMCQNCKNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVDLLVGPGAAQAAIKEDPWNCYMCGHKGTYGLLRRREDWPSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKHIQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFENVVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV

Target ORF information:

RefSeq Version	XM_017594266.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DNA methyltransferase 3 alpha (Dnmt3a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594266.1

ORF Insert Sequence:

ATGCCCTCCA GCGGCCCCGG GGACACCAGC ATCTCCTCTC TGGAGCGGGA GGATGATCGA 
AAGGAAGGAG AGGAACAGGA GGAGAACCGT GGTAAGGAAG AGCGTCAGGA GCCCAGCGCC 
ACGGCCCGGA AAGTGGGGAG GCCTGGCCGG AAGCGCAAGC ACCCACCGGT GGAAAGCAGT 
GACACCCCCA AGGACCCAGC GGTGACCACC AAGTCTCAGC CCACAGCCCA GGACTCTGGG 
CCCTCAGATC TGCTACCCAA TGGAGACTTG GAGAAGCGGA GTGAACCCCA ACCTGAGGAG 
GGGAGCCCAG CTGCAGGGCA GAAGGGTGGG GCCCCAGCTG AAGGAGAGGG AACTGAGACC 
CCACCAGAAG CCTCCAGAGC AGTGGAGAAT GGCTGCTGCG TAACCAAGGA AGGCCGTGGA 
GCCTCTGCGG GAGAGGGCAA AGAACAGAAG CAGACCAACA TCGAATCCAT GAAAATGGAG 
GGCTCCCGGG GCCGACTGCG TGGTGGCTTG GGGTGGGAGT CCAGCCTCCG TCAGCGGCCC 
ATGCCAAGAC TCACCTTCCA GGCAGGGGAC CCCTACTACA TCAGCAAACG GAAGCGGGAT 
GAGTGGCTGG CACGTTGGAA AAGGGAGGCT GAGAAGAAAG CCAAGGTGAT TGCAGTAATG 
AATGCTGTGG AGGAAAGCCA GGCCTCTGGG GAGTCCCAGA AGGTGGAGGA GGCCAGCCCT 
CCTGCAGTGC AGCAGCCCAC GGACCCTGCA TCTCCCACTG TGGCCACCAC CCCTGAGCCA 
GTAGGGGCTG ATGCTGGGGA CAAGAATGCT ACCAAGGCAG CTGATGACGA GCCCGAGTAT 
GAGGATGGCC GGGGCTTTGG CATTGGAGAG CTGGTGTGGG GGAAACTCCG GGGCTTCTCC 
TGGTGGCCAG GCCGAATTGT GTCTTGGTGG ATGACAGGCC GGAGCCGAGC AGCCGAAGGC 
ACTCGCTGGG TCATGTGGTT CGGAGATGGC AAATTCTCAG TGGTGTGTGT CGAGAAGCTC 
ATGCCCCTGA GCTCCTTCTG CAGTGCGTTC CACCAGGCCA CCTACAACAA GCAGCCCATG 
TACCGCAAAG CCATCTACGA AGTCCTCCAG GTGGCCAGCA GCCGTGCAGG GAAGCTGTTC 
CCAGCATGTC ATGACAGCGA TGAAAGTGAC ACTGGCAAGG CTGTGGAGGT GCAGAACAAG 
CAGATGATTG AGTGGGCCCT TGGCGGCTTC CAGCCCTCTG GTCCCAAAGG CCTGGAGCCA 
CCAGAAGAGG AGAAGAATCC TTACAAGGAA GTTTACACCG ACATGTGGGT GGAGCCTGAG 
GCAGCTGCTT ATGCCCCACC CCCACCAGCC AAGAAACCCA GAAAGAGCAC AACAGAGAAA 
CCCAAGGTCA AGGAGATCAT TGATGAACGC ACAAGAGAAC GGCTCGTGTA TGAGGTGCGC 
CAGAAGTGCC GAAACATCGA GGACATTTGT ATCTCATGTG GGAGCCTCAA TGTTACCCTG 
GAGCACCCAC TCTTCATTGG TGGAATGTGC CAGAACTGTA AGAACTGCTT CTTGGAGTGT 
GCTTACCAAT ACGATGACGA TGGGTACCAG TCCTACTGTA CCATCTGCTG TGGGGGGCGC 
GAAGTGCTCA TGTGTGGGAA CAACAACTGC TGCAGGTGCT TTTGTGTGGA GTGTGTGGAT 
CTCTTGGTGG GGCCAGGGGC TGCCCAAGCA GCCATTAAGG AAGACCCCTG GAACTGCTAC 
ATGTGTGGGC ACAAGGGCAC GTATGGGCTG CTGCGGAGAC GGGAGGACTG GCCTTCTCGA 
CTCCAGATGT TCTTCGCCAA TAACCACGAC CAGGAATTTG ACCCCCCGAA GGTTTACCCA 
CCTGTGCCAG CTGAGAAGAG GAAGCCCATC CGGGTGCTAT CTCTCTTTGA TGGGATTGCT 
ACAGGGCTCC TGGTGCTGAA GGACCTGGGC ATCCAAGTGG ACCGCTACAT CGCCTCCGAG 
GTGTGCGAGG ACTCCATCAC AGTGGGCATG GTGCGGCACC AGGGAAAGAT CATGTACGTC 
GGGGACGTCC GCAGCGTCAC ACAGAAGCAT ATCCAGGAGT GGGGCCCATT CGATCTGGTG 
ATTGGGGGCA GTCCCTGCAA TGACCTCTCC ATCGTCAACC CTGCCCGCAA GGGACTTTAC 
GAGGGCACGG GCCGCCTCTT CTTTGAGTTC TACCGCCTCC TGCATGACGC GCGGCCCAAG 
GAGGGAGATG ATCGCCCCTT CTTCTGGCTT TTTGAGAATG TGGTGGCCAT GGGCGTTAGT 
GACAAGAGGG ACATCTCGAG ATTTCTTGAG TCTAACCCCG TGATGATCGA CGCCAAAGAA 
GTGTCTGCTG CACACAGGGC CCGTTATTTC TGGGGTAACC TTCCTGGCAT GAACAGGCCA 
TTGGCATCCA CTGTGAATGA TAAGCTGGAG TTGCAAGAGT GTCTGGAACA CGGCAGAATA 
GCCAAGTTCA GCAAAGTGAG GACCATTACC ACCAGGTCAA ACTCCATAAA GCAGGGCAAA 
GACCAGCATT TCCCTGTCTT CATGAATGAG AAGGAAGACA TCCTCTGGTG CACTGAAATG 
GAAAGGGTGT TTGGCTTCCC TGTCCACTAC ACAGACGTCT CCAACATGAG CCGCTTGGCG 
AGGCAGAGAC TGCTGGGCCG ATCGTGGAGC GTGCCAGTCA TCCGCCACCT CTTCGCTCCG 
CTGAAGGAAT ATTTTGCTTG TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nerve injury-induced epigenetic silencing of opioid receptors controlled by DNMT3a in primary afferent neurons.
Pain158(6)1153-1165(2017 Jun)
Sun L,Zhao JY,Gu X,Liang L,Wu S,Mo K,Feng J,Guo W,Zhang J,Bekker A,Zhao X,Nestler EJ,Tao YX

DNA methyltransferase DNMT3a contributes to neuropathic pain by repressing Kcna2 in primary afferent neurons.
Nature communications814712(2017 Mar)
Zhao JY,Liang L,Gu X,Li Z,Wu S,Sun L,Atianjoh FE,Feng J,Mo K,Jia S,Lutz BM,Bekker A,Nestler EJ,Tao YX

[EXPRESS] DNMT3a contributes to the development and maintenance of bone cancer pain by silencing Kv1.2 expression in spinal cord dorsal horn.
Molecular pain131744806917740681(2017 Jan-Dec)
Miao XR,Fan LC,Wu S,Mao Q,Li Z,Lutz B,Xu JT,Lu Z,Tao YX

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Dnmt3a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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