Gjb2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gjb2
Entrez Gene ID	394266
Full Name	gap junction protein, beta 2
Synonyms	CXN-26,Cx26
General protein information	Preferred Names gap junction protein, beta 2 Names gap junction beta-2 protein connexin 26 gap junction channel protein connexin 26 gap junction membrane channel protein beta 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p12
Summary	forms gap junctions; may play a role in epididymal development [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017599765.1	XP_017455254.1	gap junction beta-2 protein isoform X1
NM_001004099.2	NP_001004099.1	gap junction beta-2 protein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GJB2	XP_522618.1
Rattus norvegicus (Norway rat)	Gjb2	NP_001004099.1
Gallus gallus (chicken)	GJB2	NP_001257745.1
Homo sapiens (human)	GJB2	NP_003995.2
Canis lupus familiaris (dog)	GJB2	NP_001184077.1
Mus musculus (house mouse)	Gjb2	NP_032151.1
	GJB2	NP_001038200.1
Bos taurus (cattle)	GJB2	NP_001077106.1

Gene Ontology
Bibliography

Related articles in PubMed

Chronic prenatal hypoxia impairs cochlear development, a mechanism involving connexin26 expression and promoter methylation.
Lin J, Huang H, Lv G, Xu X, Lin W, Xu X, Cheng J, Zheng M
International journal of molecular medicine41(2)852-858(2018 Feb)

Vitamin E and caloric restriction promote hepatic homeostasis through expression of connexin 26, N-cad, E-cad and cholesterol metabolism genes.
Santolim LV, Amaral MECD, Fachi JL, Mendes MF, Oliveira CA
The Journal of nutritional biochemistry3986-92(2017 Jan)

Charged Residues at the First Transmembrane Region Contribute to the Voltage Dependence of the Slow Gate of Connexins.
Pinto BI, Garc?a IE, Pupo A, Retamal MA, Mart?nez AD, Latorre R, Gonz?lez C
The Journal of biological chemistry291(30)15740-52(2016 07)

Role of Specificity Protein-1 and Activating Protein-2 Transcription Factors in the Regulation of the Gap Junction Protein Beta-2 Gene in the Epididymis of the Rat.
Adam C, Cyr DG
Biology of reproduction94(6)120(2016 06)

An electrostatic mechanism for Ca(2+)-mediated regulation of gap junction channels.
Bennett BC, Purdy MD, Baker KA, Acharya C, McIntire WE, Stevens RC, Zhang Q, Harris AL, Abagyan R, Yeager M
Nature communications78770(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Supplementation with vitamin E, with or without caloric restriction, upregulated the mRNA expression of Cx26, N-cad, E-cad and genes involved in cholesterol metabolism, suggesting the maintenance of hepatic homeostasis.
Title: Vitamin E and caloric restriction promote hepatic homeostasis through expression of connexin 26, N-cad, E-cad and cholesterol metabolism genes.

the role of the charged residues at the end of TM-1 in voltage sensing in Cx26, Cx46, and Cx50.
Title: Charged Residues at the First Transmembrane Region Contribute to the Voltage Dependence of the Slow Gate of Connexins.

Increased Rat Placental Fatty Acid, but Decreased Amino Acid and Glucose Transporters Potentially Modify Intrauterine Programming
Title: Increased Rat Placental Fatty Acid, but Decreased Amino Acid and Glucose Transporters Potentially Modify Intrauterine Programming.

SP1 and TFAP2A regulate Gjb2 promoter activity during epididymal differentiation in rat.
Title: Role of Specificity Protein-1 and Activating Protein-2 Transcription Factors in the Regulation of the Gap Junction Protein Beta-2 Gene in the Epididymis of the Rat.

the surface topography of Cx26 hemichannels
Title: Atomic force microscopy shows connexin26 hemichannel clustering in purified membrane fragments.

The following Gjb2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gjb2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13050	NM_001004099.2 Latest version!	Rattus norvegicus gap junction protein, beta 2 (Gjb2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
ORa13050	XM_017599765.1 Latest version!	Rattus norvegicus gap junction protein, beta 2 (Gjb2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gjb2 ( NM_001004099.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001004099.1 Rattus norvegicus gap junction protein, beta 2 (Gjb2), mRNA.

Price & Availability↑

CloneID	ORa13050
Clone ID Related Accession (Same CDS sequence)	XM_017599765.1 , NM_001004099.2
Accession Version	NM_001004099.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Rattus norvegicus(Norway rat)
Product	gap junction beta-2 protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07018050.1 and BC078952.1. On Sep 24, 2016 this sequence version replaced NM_001004099.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGGATTGGG GCACACTACA GAGCATCCTC GGGGGTGTCA ACAAGCACTC CACCAGCATT 
GGGAAAATCT GGCTCACTGT CCTCTTCATC TTCCGCATCA TGATCCTCGT GGTGGCCGCG 
AAGGAGGTGT GGGGAGATGA GCAAGCCGAT TTTGTTTGCA ACACTCTCCA GCCTGGCTGT 
AAGAATGTGT GCTACGACCA CTACTTCCCC ATCTCTCACA TCCGGCTCTG GGCTCTGCAG 
CTGATCATGG TGTCCACGCC GGCCCTCCTG GTAGCTATGC ACGTGGCCTA CCGGAGACAC 
GAAAAGAAAC GGAAGTTCAT GAAGGGAGAG ATAAAGAACG AGTTTAAGGA CATCGAAGAG 
ATCAAAACCC AGAAGGTCCG TATCGAAGGG TCCCTGTGGT GGACCTACAC CACCAGCATC 
TTCTTCCGGG TCATCTTCGA AGCTGTCTTC ATGTATGTCT TTTACATCAT GTACAATGGC 
TTCTTCATGC AGCGTCTGGT GAAGTGTAAC GCCTGGCCTT GTCCCAATAC AGTGGACTGC 
TTCATTTCCA GGCCCACAGA AAAGACTGTC TTCACGGTGT TCATGATCTC TGTGTCTGGA 
ATTTGCATCC TGCTAAACAT CACAGAGCTG TGCTATCTGT TCATTAGGTA TTGCTCAGGG 
AAGTCCAAAA GACCAGTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001004099.1
CDS	290..970
Translation	MDWGTLQSILGGVNKHSTSIGKIWLTVLFIFRIMILVVAAKEVWGDEQADFVCNTLQPGCKNVCYDHYFPISHIRLWALQLIMVSTPALLVAMHVAYRRHEKKRKFMKGEIKNEFKDIEEIKTQKVRIEGSLWWTYTTSIFFRVIFEAVFMYVFYIMYNGFFMQRLVKCNAWPCPNTVDCFISRPTEKTVFTVFMISVSGICILLNITELCYLFIRYCSGKSKRPV

Target ORF information:

RefSeq Version	NM_001004099.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus gap junction protein, beta 2 (Gjb2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001004099.2

ORF Insert Sequence:

ATGGATTGGG GCACACTACA GAGCATCCTC GGGGGTGTCA ACAAGCACTC CACCAGCATT 
GGGAAAATCT GGCTCACTGT CCTCTTCATC TTCCGCATCA TGATCCTCGT GGTGGCCGCG 
AAGGAGGTGT GGGGAGATGA GCAAGCCGAT TTTGTTTGCA ACACTCTCCA GCCTGGCTGT 
AAGAATGTGT GCTACGACCA CTACTTCCCC ATCTCTCACA TCCGGCTCTG GGCTCTGCAG 
CTGATCATGG TGTCCACGCC GGCCCTCCTG GTAGCTATGC ACGTGGCCTA CCGGAGACAC 
GAAAAGAAAC GGAAGTTCAT GAAGGGAGAG ATAAAGAACG AGTTTAAGGA CATCGAAGAG 
ATCAAAACCC AGAAGGTCCG TATCGAAGGG TCCCTGTGGT GGACCTACAC CACCAGCATC 
TTCTTCCGGG TCATCTTCGA AGCTGTCTTC ATGTATGTCT TTTACATCAT GTACAATGGC 
TTCTTCATGC AGCGTCTGGT GAAGTGTAAC GCCTGGCCTT GTCCCAATAC AGTGGACTGC 
TTCATTTCCA GGCCCACAGA AAAGACTGTC TTCACGGTGT TCATGATCTC TGTGTCTGGA 
ATTTGCATCC TGCTAAACAT CACAGAGCTG TGCTATCTGT TCATTAGGTA TTGCTCAGGG 
AAGTCCAAAA GACCAGTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gjb2 ( XM_017599765.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455254.1 Rattus norvegicus gap junction protein, beta 2 (Gjb2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa13050
Clone ID Related Accession (Same CDS sequence)	XM_017599765.1 , NM_001004099.2
Accession Version	XM_017599765.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	gap junction beta-2 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTGGG GCACACTACA GAGCATCCTC GGGGGTGTCA ACAAGCACTC CACCAGCATT 
GGGAAAATCT GGCTCACTGT CCTCTTCATC TTCCGCATCA TGATCCTCGT GGTGGCCGCG 
AAGGAGGTGT GGGGAGATGA GCAAGCCGAT TTTGTTTGCA ACACTCTCCA GCCTGGCTGT 
AAGAATGTGT GCTACGACCA CTACTTCCCC ATCTCTCACA TCCGGCTCTG GGCTCTGCAG 
CTGATCATGG TGTCCACGCC GGCCCTCCTG GTAGCTATGC ACGTGGCCTA CCGGAGACAC 
GAAAAGAAAC GGAAGTTCAT GAAGGGAGAG ATAAAGAACG AGTTTAAGGA CATCGAAGAG 
ATCAAAACCC AGAAGGTCCG TATCGAAGGG TCCCTGTGGT GGACCTACAC CACCAGCATC 
TTCTTCCGGG TCATCTTCGA AGCTGTCTTC ATGTATGTCT TTTACATCAT GTACAATGGC 
TTCTTCATGC AGCGTCTGGT GAAGTGTAAC GCCTGGCCTT GTCCCAATAC AGTGGACTGC 
TTCATTTCCA GGCCCACAGA AAAGACTGTC TTCACGGTGT TCATGATCTC TGTGTCTGGA 
ATTTGCATCC TGCTAAACAT CACAGAGCTG TGCTATCTGT TCATTAGGTA TTGCTCAGGG 
AAGTCCAAAA GACCAGTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455254.1
CDS	103..783
Translation	MDWGTLQSILGGVNKHSTSIGKIWLTVLFIFRIMILVVAAKEVWGDEQADFVCNTLQPGCKNVCYDHYFPISHIRLWALQLIMVSTPALLVAMHVAYRRHEKKRKFMKGEIKNEFKDIEEIKTQKVRIEGSLWWTYTTSIFFRVIFEAVFMYVFYIMYNGFFMQRLVKCNAWPCPNTVDCFISRPTEKTVFTVFMISVSGICILLNITELCYLFIRYCSGKSKRPV

Target ORF information:

RefSeq Version	XM_017599765.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus gap junction protein, beta 2 (Gjb2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599765.1

ORF Insert Sequence:

ATGGATTGGG GCACACTACA GAGCATCCTC GGGGGTGTCA ACAAGCACTC CACCAGCATT 
GGGAAAATCT GGCTCACTGT CCTCTTCATC TTCCGCATCA TGATCCTCGT GGTGGCCGCG 
AAGGAGGTGT GGGGAGATGA GCAAGCCGAT TTTGTTTGCA ACACTCTCCA GCCTGGCTGT 
AAGAATGTGT GCTACGACCA CTACTTCCCC ATCTCTCACA TCCGGCTCTG GGCTCTGCAG 
CTGATCATGG TGTCCACGCC GGCCCTCCTG GTAGCTATGC ACGTGGCCTA CCGGAGACAC 
GAAAAGAAAC GGAAGTTCAT GAAGGGAGAG ATAAAGAACG AGTTTAAGGA CATCGAAGAG 
ATCAAAACCC AGAAGGTCCG TATCGAAGGG TCCCTGTGGT GGACCTACAC CACCAGCATC 
TTCTTCCGGG TCATCTTCGA AGCTGTCTTC ATGTATGTCT TTTACATCAT GTACAATGGC 
TTCTTCATGC AGCGTCTGGT GAAGTGTAAC GCCTGGCCTT GTCCCAATAC AGTGGACTGC 
TTCATTTCCA GGCCCACAGA AAAGACTGTC TTCACGGTGT TCATGATCTC TGTGTCTGGA 
ATTTGCATCC TGCTAAACAT CACAGAGCTG TGCTATCTGT TCATTAGGTA TTGCTCAGGG 
AAGTCCAAAA GACCAGTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chronic prenatal hypoxia impairs cochlear development, a mechanism involving connexin26 expression and promoter methylation.
International journal of molecular medicine41(2)852-858(2018 Feb)
Lin J,Huang H,Lv G,Xu X,Lin W,Xu X,Cheng J,Zheng M

Vitamin E and caloric restriction promote hepatic homeostasis through expression of connexin 26, N-cad, E-cad and cholesterol metabolism genes.
The Journal of nutritional biochemistry3986-92(2017 Jan)
Santolim LV,Amaral MECD,Fachi JL,Mendes MF,Oliveira CA

Charged Residues at the First Transmembrane Region Contribute to the Voltage Dependence of the Slow Gate of Connexins.
The Journal of biological chemistry291(30)15740-52(2016 07)
Pinto BI,Garc?a IE,Pupo A,Retamal MA,Mart?nez AD,Latorre R,Gonz?lez C

Role of Specificity Protein-1 and Activating Protein-2 Transcription Factors in the Regulation of the Gap Junction Protein Beta-2 Gene in the Epididymis of the Rat.
Biology of reproduction94(6)120(2016 06)
Adam C,Cyr DG

An electrostatic mechanism for Ca(2+)-mediated regulation of gap junction channels.
Nature communications78770(2016 Jan)
Bennett BC,Purdy MD,Baker KA,Acharya C,McIntire WE,Stevens RC,Zhang Q,Harris AL,Abagyan R,Yeager M

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Gjb2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム