Qrfp cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Qrfp
Entrez Gene ID	379044
Full Name	pyroglutamylated RFamide peptide
Synonyms	26RFa,P518
General protein information	Preferred Names pyroglutamylated RFamide peptide Names orexigenic neuropeptide QRFP P518 precursor protein neuropeptide
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3p12
Summary	peptide ligand for G-protein coupled receptor AQ27 [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_003749440.3	XP_003749488.1	orexigenic neuropeptide QRFP isoform X1
NM_198200.1	NP_937843.1	orexigenic neuropeptide QRFP precursor

Pathways from BioSystems

Homologies

	QRFP	XP_001106500.1
Canis lupus familiaris (dog)	QRFP	XP_850751.2
Bos taurus (cattle)	QRFP	NP_937865.1
Rattus norvegicus (Norway rat)	LOC100909783	XP_003749488.1
Pan troglodytes (chimpanzee)	QRFP	XP_001166241.3
Mus musculus (house mouse)	Qrfp	NP_906269.1
Homo sapiens (human)	QRFP	NP_937823.1
Rattus norvegicus (Norway rat)	Qrfp	NP_937843.1

Gene Ontology
Bibliography

Related articles in PubMed

The effects of high fat diet and estradiol on hypothalamic prepro-QRFP mRNA expression in female rats.
Schreiber AL, Arceneaux KP 3rd, Malbrue RA, Mouton AJ, Chen CS, Bench EM, Braymer HD, Primeaux SD
Neuropeptides58103-9(2016 Aug)

Hypothalamic QRFP: regulation of food intake and fat selection.
Primeaux SD, Barnes MJ, Braymer HD
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme45(13)967-74(2013 Dec)

QRFP in female rats: effects on high fat food intake and hypothalamic gene expression across the estrous cycle.
Primeaux SD
Peptides32(6)1270-5(2011 Jun)

Intracerebroventricular administration of 26RFa produces an analgesic effect in the rat formalin test.
Yamamoto T, Miyazaki R, Yamada T
Peptides30(9)1683-8(2009 Sep)

Suppression of QRFP 43 in the hypothalamic ventromedial nucleus of Long-Evans rats fed a high-fat diet.
Beck B, Richy S
Biochemical and biophysical research communications383(1)78-82(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies support a role for estradiol (E2) in the regulation of prepro-QRFP mRNA in the ventral medial nucleus of the hypothalamus/arcuate nucleus and suggest that E2's effects on food intake may be via a direct effect on the orexigenic peptide, QRFP.
Title: The effects of high fat diet and estradiol on hypothalamic prepro-QRFP mRNA expression in female rats.

The present review discusses the role of QRFP in the regulation of feeding behavior, with emphasis on the intake of dietary fat.
Title: Hypothalamic QRFP: regulation of food intake and fat selection.

QRFP may play a role in coordinating feeding behaviors with reproductive function in rats when energy demand is increased.
Title: QRFP in female rats: effects on high fat food intake and hypothalamic gene expression across the estrous cycle.

Data suggest that the GPR103-26RFa system modulates nociceptive transmission in both the spinal cord and the supraspinal brain in the formalin (paw) test.
Title: Intracerebroventricular administration of 26RFa produces an analgesic effect in the rat formalin test.

QRFP 43 might play a role downstream of leptin in the regulation of feeding behavior.
Title: Suppression of QRFP 43 in the hypothalamic ventromedial nucleus of Long-Evans rats fed a high-fat diet.

The following Qrfp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Qrfp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa15522	NM_198200.1 Latest version!	Rattus norvegicus pyroglutamylated RFamide peptide (Qrfp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa15522	XM_003749440.3 Latest version!	Rattus norvegicus pyroglutamylated RFamide peptide (Qrfp), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Qrfp ( NM_198200.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_937843.1 Rattus norvegicus pyroglutamylated RFamide peptide (Qrfp), mRNA.

Price & Availability↑

CloneID	ORa15522
Clone ID Related Accession (Same CDS sequence)	XM_003749440.3 , NM_198200.1
Accession Version	NM_198200.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	orexigenic neuropeptide QRFP precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB109627.1. ##Evidence-Data-START## Transcript exon combination :: AB109627.1 [ECO:0000332] ##Evidence-Data-END##

ATGAGGTGCC TCTGCTCTTG GCTTTGCCTC CTCCTGCCTC TGAGTGCCTG CTTTCCTCTG 
CTGGACAGAA GGGGACCCAC AGACATCGGT GACATCGGAG CCAGAATGAG CTGGGTCCAG 
CTGACTGAGG GACACACCCC CCGCTCAGTT CAAAGTCCAC GGCCACAGGC CCTGCTCGTG 
GTGGCCAAGG AGCAGCAGGC CTCTCGCAGG GAGCACACTG GCTTCCGTCT AGGGAGGCAG 
GACAGTGGCA GTGAAGCCAC GGGGTTCCTG CCCACTGACT CAGAGAAGGC CAGCGGCCCC 
CTGGGGACTC TGGCAGAGGA GCTCAGCAGC TACAGCCGGC GGAAGGGAGG CTTCAGCTTC 
CGCTTCGGCC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_937843.1
CDS	1..375
Translation	MRCLCSWLCLLLPLSACFPLLDRRGPTDIGDIGARMSWVQLTEGHTPRSVQSPRPQALLVVAKEQQASRREHTGFRLGRQDSGSEATGFLPTDSEKASGPLGTLAEELSSYSRRKGGFSFRFGR

Target ORF information:

RefSeq Version	NM_198200.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pyroglutamylated RFamide peptide (Qrfp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198200.1

ORF Insert Sequence:

ATGAGGTGCC TCTGCTCTTG GCTTTGCCTC CTCCTGCCTC TGAGTGCCTG CTTTCCTCTG 
CTGGACAGAA GGGGACCCAC AGACATCGGT GACATCGGAG CCAGAATGAG CTGGGTCCAG 
CTGACTGAGG GACACACCCC CCGCTCAGTT CAAAGTCCAC GGCCACAGGC CCTGCTCGTG 
GTGGCCAAGG AGCAGCAGGC CTCTCGCAGG GAGCACACTG GCTTCCGTCT AGGGAGGCAG 
GACAGTGGCA GTGAAGCCAC GGGGTTCCTG CCCACTGACT CAGAGAAGGC CAGCGGCCCC 
CTGGGGACTC TGGCAGAGGA GCTCAGCAGC TACAGCCGGC GGAAGGGAGG CTTCAGCTTC 
CGCTTCGGCC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Qrfp ( XM_003749440.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_003749488.1 Rattus norvegicus pyroglutamylated RFamide peptide (Qrfp), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa15522
Clone ID Related Accession (Same CDS sequence)	XM_003749440.3 , NM_198200.1
Accession Version	XM_003749440.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	orexigenic neuropeptide QRFP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_003749440.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTGCC TCTGCTCTTG GCTTTGCCTC CTCCTGCCTC TGAGTGCCTG CTTTCCTCTG 
CTGGACAGAA GGGGACCCAC AGACATCGGT GACATCGGAG CCAGAATGAG CTGGGTCCAG 
CTGACTGAGG GACACACCCC CCGCTCAGTT CAAAGTCCAC GGCCACAGGC CCTGCTCGTG 
GTGGCCAAGG AGCAGCAGGC CTCTCGCAGG GAGCACACTG GCTTCCGTCT AGGGAGGCAG 
GACAGTGGCA GTGAAGCCAC GGGGTTCCTG CCCACTGACT CAGAGAAGGC CAGCGGCCCC 
CTGGGGACTC TGGCAGAGGA GCTCAGCAGC TACAGCCGGC GGAAGGGAGG CTTCAGCTTC 
CGCTTCGGCC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003749488.1
CDS	14..388
Translation	MRCLCSWLCLLLPLSACFPLLDRRGPTDIGDIGARMSWVQLTEGHTPRSVQSPRPQALLVVAKEQQASRREHTGFRLGRQDSGSEATGFLPTDSEKASGPLGTLAEELSSYSRRKGGFSFRFGR

Target ORF information:

RefSeq Version	XM_003749440.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pyroglutamylated RFamide peptide (Qrfp), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003749440.3

ORF Insert Sequence:

ATGAGGTGCC TCTGCTCTTG GCTTTGCCTC CTCCTGCCTC TGAGTGCCTG CTTTCCTCTG 
CTGGACAGAA GGGGACCCAC AGACATCGGT GACATCGGAG CCAGAATGAG CTGGGTCCAG 
CTGACTGAGG GACACACCCC CCGCTCAGTT CAAAGTCCAC GGCCACAGGC CCTGCTCGTG 
GTGGCCAAGG AGCAGCAGGC CTCTCGCAGG GAGCACACTG GCTTCCGTCT AGGGAGGCAG 
GACAGTGGCA GTGAAGCCAC GGGGTTCCTG CCCACTGACT CAGAGAAGGC CAGCGGCCCC 
CTGGGGACTC TGGCAGAGGA GCTCAGCAGC TACAGCCGGC GGAAGGGAGG CTTCAGCTTC 
CGCTTCGGCC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The effects of high fat diet and estradiol on hypothalamic prepro-QRFP mRNA expression in female rats.
Neuropeptides58103-9(2016 Aug)
Schreiber AL,Arceneaux KP 3rd,Malbrue RA,Mouton AJ,Chen CS,Bench EM,Braymer HD,Primeaux SD

Hypothalamic QRFP: regulation of food intake and fat selection.
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme45(13)967-74(2013 Dec)
Primeaux SD,Barnes MJ,Braymer HD

QRFP in female rats: effects on high fat food intake and hypothalamic gene expression across the estrous cycle.
Peptides32(6)1270-5(2011 Jun)
Primeaux SD

Intracerebroventricular administration of 26RFa produces an analgesic effect in the rat formalin test.
Peptides30(9)1683-8(2009 Sep)
Yamamoto T,Miyazaki R,Yamada T

Suppression of QRFP 43 in the hypothalamic ventromedial nucleus of Long-Evans rats fed a high-fat diet.
Biochemical and biophysical research communications383(1)78-82(2009 May)
Beck B,Richy S