Polr3k cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Polr3k
Entrez Gene ID	366277
Full Name	RNA polymerase III subunit K
General protein information	Preferred Names RNA polymerase III subunit K Names DNA-directed RNA polymerase III subunit RPC10 estrous-specific protein, 250 kDa polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa polymerase (RNA) III subunit K
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q43
Summary	estrogen responsive protein; may be regulated by 17beta-estradiol [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001014259.1	NP_001014281.1	DNA-directed RNA polymerase III subunit RPC10

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Polr3k	NP_080177.1
Danio rerio (zebrafish)	polr3k	NP_001002553.1
Xenopus tropicalis (tropical clawed frog)	polr3k	NP_001015803.1
Pan troglodytes (chimpanzee)	POLR3K	XP_001150299.1
Kluyveromyces lactis	KLLA0F18568g	XP_455912.1
Saccharomyces cerevisiae (baker's yeast)	RPC11	NP_010330.1
Bos taurus (cattle)	POLR3K	NP_001040082.1
Rattus norvegicus (Norway rat)	Polr3k	NP_001014281.1
Drosophila melanogaster (fruit fly)	CG33785	NP_001027443.1
Schizosaccharomyces pombe (fission yeast)	rpc11	NP_593235.1
Magnaporthe oryzae (rice blast fungus)	MGG_03072	XP_003720603.1
Neurospora crassa	NCU00570	XP_964731.1
Homo sapiens (human)	POLR3K	NP_057394.2
Caenorhabditis elegans (roundworm)	Y77E11A.6	NP_500076.1
Eremothecium gossypii	AGOS_AGR029W	NP_986694.1

Gene Ontology
Bibliography

Related articles in PubMed

Cell growth- and differentiation-dependent regulation of RNA polymerase III transcription.
Dumay-Odelot H, Durrieu-Gaillard S, Da Silva D, Roeder RG, Teichmann M
Cell cycle (Georgetown, Tex.)9(18)3687-99(2010 Sep)

Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits.
Hu P, Wu S, Sun Y, Yuan CC, Kobayashi R, Myers MP, Hernandez N
Molecular and cellular biology22(22)8044-55(2002 Nov)

Characterization of a uterine luminal fluid protein ULF-250 using N-terminal microsequencing and RT-PCR identifies a novel estrogen-regulated gene in the rat uterus.
Kumar G P, Laloraya M, Koide SS
FEBS letters399(1-2)33-6(1996 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Polr3k gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Polr3k cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05557	NM_001014259.1 Latest version!	Rattus norvegicus RNA polymerase III subunit K (Polr3k), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Polr3k ( NM_001014259.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001014281.1 Rattus norvegicus RNA polymerase III subunit K (Polr3k), mRNA.

Price & Availability↑

CloneID	ORa05557
Clone ID Related Accession (Same CDS sequence)	NM_001014259.1
Accession Version	NM_001014259.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	DNA-directed RNA polymerase III subunit RPC10
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC089990.1. On Apr 1, 2005 this sequence version replaced XM_345487.1. ##Evidence-Data-START## Transcript exon combination :: BC089990.1, FM076686.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCTGCTGT TCTGCCCGGG CTGCGGGAAC GGCCTCATTG TGGAGGAGGG GCAGCGCTGT 
CACCGCTTCG CCTGCAACAC GTGCCCGTAC GTCCATAACA TCACCCGCAA GGTAACAAAC 
CGAAAGTATC CAAAGCTAAA AGAAGTGGAT GATGTGCTTG GTGGAGCAGC TGCCTGGGAG 
AATGTGGACT CTACTGCAGA GCCATGTCCA AAATGTGAGC ATCCTCGTGC CTACTTCATG 
CAGCTTCAGA CCCGTTCTGC GGACGAGCCA ATGACCACCT TCTATAAGTG CTGCAATGCT 
CAGTGTGGAC ATCGCTGGAG GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014281.1
CDS	65..391
Translation	MLLFCPGCGNGLIVEEGQRCHRFACNTCPYVHNITRKVTNRKYPKLKEVDDVLGGAAAWENVDSTAEPCPKCEHPRAYFMQLQTRSADEPMTTFYKCCNAQCGHRWRD

Target ORF information:

RefSeq Version	NM_001014259.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RNA polymerase III subunit K (Polr3k), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014259.1

ORF Insert Sequence:

ATGCTGCTGT TCTGCCCGGG CTGCGGGAAC GGCCTCATTG TGGAGGAGGG GCAGCGCTGT 
CACCGCTTCG CCTGCAACAC GTGCCCGTAC GTCCATAACA TCACCCGCAA GGTAACAAAC 
CGAAAGTATC CAAAGCTAAA AGAAGTGGAT GATGTGCTTG GTGGAGCAGC TGCCTGGGAG 
AATGTGGACT CTACTGCAGA GCCATGTCCA AAATGTGAGC ATCCTCGTGC CTACTTCATG 
CAGCTTCAGA CCCGTTCTGC GGACGAGCCA ATGACCACCT TCTATAAGTG CTGCAATGCT 
CAGTGTGGAC ATCGCTGGAG GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cell growth- and differentiation-dependent regulation of RNA polymerase III transcription.
Cell cycle (Georgetown, Tex.)9(18)3687-99(2010 Sep)
Dumay-Odelot H,Durrieu-Gaillard S,Da Silva D,Roeder RG,Teichmann M

Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits.
Molecular and cellular biology22(22)8044-55(2002 Nov)
Hu P,Wu S,Sun Y,Yuan CC,Kobayashi R,Myers MP,Hernandez N

Characterization of a uterine luminal fluid protein ULF-250 using N-terminal microsequencing and RT-PCR identifies a novel estrogen-regulated gene in the rat uterus.
FEBS letters399(1-2)33-6(1996 Dec)
Kumar G P,Laloraya M,Koide SS