Iars2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Iars2
Entrez Gene ID	364070
Full Name	isoleucyl-tRNA synthetase 2, mitochondrial
Synonyms	RGD1311857
General protein information	Preferred Names isoleucyl-tRNA synthetase 2, mitochondrial Names isoleucyl-tRNA synthetase, mitochondrial LOW QUALITY PROTEIN: isoleucine--tRNA ligase, mitochondrial isoleucine--tRNA ligase, mitochondrial isoleucine-tRNA synthetase 2, mitochondrial mitochondrial isoleucine tRNA synthetase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q26

mRNA and Protein(s)

mRNA	Protein	Name
XM_017604692.1	XP_017460181.1	isoleucine--tRNA ligase, mitochondrial isoform X2
XM_017599008.1	XP_017454497.1	LOW QUALITY PROTEIN: isoleucine--tRNA ligase, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG5414	NP_648837.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003156	XP_312846.5
Eremothecium gossypii	AGOS_AGL247C	NP_986420.1
Neurospora crassa	NCU00920	XP_964746.2
Arabidopsis thaliana (thale cress)	OVA2	NP_199714.2
Canis lupus familiaris (dog)	IARS2	XP_536121.3
Gallus gallus (chicken)	IARS2	NP_001006397.1
Saccharomyces cerevisiae (baker's yeast)	ISM1	NP_015285.1
Schizosaccharomyces pombe (fission yeast)	SPCC18B5.08c	NP_587938.1
Homo sapiens (human)	IARS2	NP_060530.3
Pan troglodytes (chimpanzee)	IARS2	XP_514209.2
	IARS2	XP_001099877.1
Danio rerio (zebrafish)	iars2	NP_001157270.1
Caenorhabditis elegans (roundworm)	iars-2	NP_001251317.1
Kluyveromyces lactis	KLLA0D11858g	XP_453590.1
Xenopus tropicalis (tropical clawed frog)	iars2	NP_001120515.1
Bos taurus (cattle)	IARS2	NP_001193724.1
Mus musculus (house mouse)	Iars2	NP_941055.1
Rattus norvegicus (Norway rat)	Iars2	XP_006250494.1
Magnaporthe oryzae (rice blast fungus)	MGG_01165	XP_003714047.1
Oryza sativa (rice)	Os02g0778200	NP_001048293.1

Gene Ontology

The following Iars2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Iars2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa54350	XM_017604692.1 Latest version!	Rattus norvegicus isoleucyl-tRNA synthetase 2, mitochondrial (Iars2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa58177	XM_017599008.1 Latest version!	Rattus norvegicus isoleucyl-tRNA synthetase 2, mitochondrial (Iars2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Iars2 ( XM_017604692.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017460181.1 Rattus norvegicus isoleucyl-tRNA synthetase 2, mitochondrial (Iars2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa54350
Clone ID Related Accession (Same CDS sequence)	XM_017604692.1
Accession Version	XM_017604692.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	isoleucine--tRNA ligase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGCTTTGGG GGCTGCACCC TCACGGACCC GGAACGGCGG CGCTCACCGC GGCTGGGAGC 
TTATGGGGGC CAGCCCGTTT TCCCAGCCGC CTGGGGTGTC TGGGGATGAC GAGGAGGCTC 
GTGGTACGCT CCGTAGCCGG AGCGGACAGC CCCCAAAGCA GCCCGAAAAG CGGCAGATAC 
CGCGACACTG TGCTGCTGCC GCAGACCAAC TTCCCGATGA AGCTGCTGGG CCACCAGCAG 
TCCGACATGG AGCTGGAGAT CCAGCAGAAA TGTGGCTTTT CAGAGCTTTA TTCCTGGCAA 
AGAGAAAGAA AAGTAAAGAC GGAATTTTGC CTTCACGATG GCCCTCCCTA TGCAAATGGT 
GACCCTCATG TTGGGCATGC CTTAAACAAG ATTTTGAAAG ACATTGTCAA TCGATTCCAT 
ATGATGAGAG GTTCCAAGGT ACACTTTGTG CCTGGCTGGG ATTGCCATGG CTTGCCCATT 
GAAACAAAAG TGTTATCAGA GCTCGGGGTA AACACCCAGA CTCTTTCAGC CATGGAGATC 
AGGGAGAAAG CCAGGTCGTT TGCTCAAGCA GCCATTGAAA AGCAGAAATC GGCGTTTGCC 
CGCTGGGGAG TGATGGCGGA TTGGAGTAAC TGCTATTACA CATTCGATCC AAAGTATGAA 
GCCAAGCAGC TGAGAGTATT TCAGCAAATG TACGAGAAGG GCTTGGTTTA TCGATCTTAC 
AAACCAGTGT ATTGGTCTCC CTCATCGAGG ACGGCGCTGG CTGAAGCAGA GCTTGAGTAC 
AACCCTGAGC ATGTTAGTCG GTCGGTGTAT GTGAGATTCC CTCTCCTGAA GCCTCCACCT 
ACGCTGGAAT CTCTCACAGG TGCCTCATCC CCTGTTAGCG CTTTAGTCTG GACCACCCAG 
CCTTGGACAG TTCCTGCCAA TCAGGCTATA TGCTACATGC CGGAGGCCAA GTACGCTGTT 
GTGAAATGTT CTGCTTCTGG AAACTTCTAC ATCCTGGCTG AAGATAAAAT ATCACCTGTT 
GCTTCTGCCT TGGAAACAAC ATTTGATGTT ACTGCAACGT TTTCAGGTGT GGATCTGGAA 
GGTGGCACTT GTAGTCACCC TTTAATTCCC GATAAAGTGT CTCCTCTTTT ACCTGCCACT 
CACGTGACTA TGGCCAAAGG AACAGGGCTG GTCCACACAG CGCCAGCTCA CGGTATGGAA 
GATTACAGCG TAGCATCGCA GCACAGCCTC CCTATGGTAC TGTCCTCTTT CTTCCCTTTA 
ATTATTCGCC TTGCAGTATG GACACCCGGA ATGGAGAGCA GGATTAGGAA AAGTCAGCAC 
AGCTCTTTAA AGTATGTTCT GCTTCTCCCA CTTGACCTGC TGATGAATGA CTTGCAGGAG 
TCGTTAAAGA CAGTGAAGTT CATTCCCGGA GCAGCACTGA ATAGCATGAT GGACATGCTG 
GACAGACGGC CGTACTGGTG CATATCAAGG CAGAGAGTCT GGGGTGTTCC AATCCCTGTC 
TTTCATCATA AGACAAAAGA TGAATATTTG ATCAACAGCC AAACCACCGA GCATATTATC 
AAACTCGTAG AGCAGCACGG CAGTGATGTG TGGTGGACCC TCCCCGCTGA GAAGCTTCTC 
CCGGAGGAAG TTGTAGCTCA GGCTGGTGGC CCCGGTGCTT TGGAGTACGT GCCAGGGCAG 
GACATTTTGG ACATCTGGTT TGACAGTGGA ACCTCGTGGT CTTGTGTTCT TCAAGGCACT 
GAGCAGAGGG CAGACTTGTA CCTGGAGGGG AAGGACCAGC TCGGGGGCTG GTTCCAGTCT 
TCGTTACTGA CAAGTGTGGC CACAAGGTCA AAAGCACCTT TCAGGACAGT GATGGTTCAT 
GGATTTACAC TCGGAGAAAA AGGAGAAAAG ATGTCCAAGT CCCTCGGGAA CGTCATTGAC 
CCTGACACTA TCATCAGTGG AGGAAAAGAC CACAGCAAAG AGCCTCCCTA CGGAGCTGAC 
ATCCTGCGCT GGTGGATAGC GGAATCCAAC GTCTTCACGG AGGTGACCAT TGGCCCCTCT 
GTACTCAGTG CTGCCAGAGA TGACATCAGC AAGCTTAGAA ACACCCTCCG CTTTCTCTTG 
GGAAATTTGG CCGGTTTTAA CCCAGAGACG GCTTCTGTTC CTGTGAAGAA TATGTACATC 
ATAGACCAGT ATATGCTGCA CCTAATTCAG GATTTTGCCA CAAAGGTTAC TGACTCATAC 
AAACAGTATG ACTTTGGAAA GGTTGTCAGG CTGTTGAAAG CCTTCTACAC CAGAGAACTG 
TCCAGCTTTT ACTTCAGCAT AGTCAAGGAT AGGCTCTACT GTGAAAGCGA GACGGATCCC 
AAGCGGCGCT CCTGTCAGAC AGCATTGGCG GAAATTCTGG ATGTGTTGGT CCGTTCTTTT 
GCGCCCGTTC TTCCTCACTT GGCAGAAGAG GTGTTCCAGC ACATCCCCTA CGTTAAAGAA 
CCCAAGAGTG TTTTTCGCAC TGGCTGGATT AGTACAAGCT CTGTTTGGAA GAAGCCAGGA 
TTGGAGGAGG CAGTGGAGAG TGCCTGTGCT ATGCGGGATT CCTTCCTTGG CAGCATCCCT 
GGAAAAAATG CTGCCGAGTA TGACGTCATC ATAGTGATCG AGCCCGGGCT GCTGTTTGAG 
ATCATGGAGA TGCTCCAGGC TGAGGAGACC TCTAGCACAT CTCAGCTGAA TGAGTTGATG 
ATGGCCTCCC AGACAACACT GTTGGCTCAG GAGCCACGGG AAAGAAGTGC AGATGCCATT 
GAGCTCACTG GGACATTCGT CATCAATCTA GAAGGTGGTG ACATTCGTGA GGAATCTTCA 
TATAAAGTCA TTGTCGTACC AACTGCCAGA GAGAAGTGCC CGCGATGTTG GAAGCATACC 
GCTGAGACTG CAGATGCTCT CTGTCCTCGG TGTGCAGAGG TCATTGGTGC AAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017460181.1
CDS	114..3050
Translation	MLWGLHPHGPGTAALTAAGSLWGPARFPSRLGCLGMTRRLVVRSVAGADSPQSSPKSGRYRDTVLLPQTNFPMKLLGHQQSDMELEIQQKCGFSELYSWQRERKVKTEFCLHDGPPYANGDPHVGHALNKILKDIVNRFHMMRGSKVHFVPGWDCHGLPIETKVLSELGVNTQTLSAMEIREKARSFAQAAIEKQKSAFARWGVMADWSNCYYTFDPKYEAKQLRVFQQMYEKGLVYRSYKPVYWSPSSRTALAEAELEYNPEHVSRSVYVRFPLLKPPPTLESLTGASSPVSALVWTTQPWTVPANQAICYMPEAKYAVVKCSASGNFYILAEDKISPVASALETTFDVTATFSGVDLEGGTCSHPLIPDKVSPLLPATHVTMAKGTGLVHTAPAHGMEDYSVASQHSLPMVLSSFFPLIIRLAVWTPGMESRIRKSQHSSLKYVLLLPLDLLMNDLQESLKTVKFIPGAALNSMMDMLDRRPYWCISRQRVWGVPIPVFHHKTKDEYLINSQTTEHIIKLVEQHGSDVWWTLPAEKLLPEEVVAQAGGPGALEYVPGQDILDIWFDSGTSWSCVLQGTEQRADLYLEGKDQLGGWFQSSLLTSVATRSKAPFRTVMVHGFTLGEKGEKMSKSLGNVIDPDTIISGGKDHSKEPPYGADILRWWIAESNVFTEVTIGPSVLSAARDDISKLRNTLRFLLGNLAGFNPETASVPVKNMYIIDQYMLHLIQDFATKVTDSYKQYDFGKVVRLLKAFYTRELSSFYFSIVKDRLYCESETDPKRRSCQTALAEILDVLVRSFAPVLPHLAEEVFQHIPYVKEPKSVFRTGWISTSSVWKKPGLEEAVESACAMRDSFLGSIPGKNAAEYDVIIVIEPGLLFEIMEMLQAEETSSTSQLNELMMASQTTLLAQEPRERSADAIELTGTFVINLEGGDIREESSYKVIVVPTAREKCPRCWKHTAETADALCPRCAEVIGAK

Target ORF information:

RefSeq Version	XM_017604692.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus isoleucyl-tRNA synthetase 2, mitochondrial (Iars2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017604692.1

ORF Insert Sequence:

ATGCTTTGGG GGCTGCACCC TCACGGACCC GGAACGGCGG CGCTCACCGC GGCTGGGAGC 
TTATGGGGGC CAGCCCGTTT TCCCAGCCGC CTGGGGTGTC TGGGGATGAC GAGGAGGCTC 
GTGGTACGCT CCGTAGCCGG AGCGGACAGC CCCCAAAGCA GCCCGAAAAG CGGCAGATAC 
CGCGACACTG TGCTGCTGCC GCAGACCAAC TTCCCGATGA AGCTGCTGGG CCACCAGCAG 
TCCGACATGG AGCTGGAGAT CCAGCAGAAA TGTGGCTTTT CAGAGCTTTA TTCCTGGCAA 
AGAGAAAGAA AAGTAAAGAC GGAATTTTGC CTTCACGATG GCCCTCCCTA TGCAAATGGT 
GACCCTCATG TTGGGCATGC CTTAAACAAG ATTTTGAAAG ACATTGTCAA TCGATTCCAT 
ATGATGAGAG GTTCCAAGGT ACACTTTGTG CCTGGCTGGG ATTGCCATGG CTTGCCCATT 
GAAACAAAAG TGTTATCAGA GCTCGGGGTA AACACCCAGA CTCTTTCAGC CATGGAGATC 
AGGGAGAAAG CCAGGTCGTT TGCTCAAGCA GCCATTGAAA AGCAGAAATC GGCGTTTGCC 
CGCTGGGGAG TGATGGCGGA TTGGAGTAAC TGCTATTACA CATTCGATCC AAAGTATGAA 
GCCAAGCAGC TGAGAGTATT TCAGCAAATG TACGAGAAGG GCTTGGTTTA TCGATCTTAC 
AAACCAGTGT ATTGGTCTCC CTCATCGAGG ACGGCGCTGG CTGAAGCAGA GCTTGAGTAC 
AACCCTGAGC ATGTTAGTCG GTCGGTGTAT GTGAGATTCC CTCTCCTGAA GCCTCCACCT 
ACGCTGGAAT CTCTCACAGG TGCCTCATCC CCTGTTAGCG CTTTAGTCTG GACCACCCAG 
CCTTGGACAG TTCCTGCCAA TCAGGCTATA TGCTACATGC CGGAGGCCAA GTACGCTGTT 
GTGAAATGTT CTGCTTCTGG AAACTTCTAC ATCCTGGCTG AAGATAAAAT ATCACCTGTT 
GCTTCTGCCT TGGAAACAAC ATTTGATGTT ACTGCAACGT TTTCAGGTGT GGATCTGGAA 
GGTGGCACTT GTAGTCACCC TTTAATTCCC GATAAAGTGT CTCCTCTTTT ACCTGCCACT 
CACGTGACTA TGGCCAAAGG AACAGGGCTG GTCCACACAG CGCCAGCTCA CGGTATGGAA 
GATTACAGCG TAGCATCGCA GCACAGCCTC CCTATGGTAC TGTCCTCTTT CTTCCCTTTA 
ATTATTCGCC TTGCAGTATG GACACCCGGA ATGGAGAGCA GGATTAGGAA AAGTCAGCAC 
AGCTCTTTAA AGTATGTTCT GCTTCTCCCA CTTGACCTGC TGATGAATGA CTTGCAGGAG 
TCGTTAAAGA CAGTGAAGTT CATTCCCGGA GCAGCACTGA ATAGCATGAT GGACATGCTG 
GACAGACGGC CGTACTGGTG CATATCAAGG CAGAGAGTCT GGGGTGTTCC AATCCCTGTC 
TTTCATCATA AGACAAAAGA TGAATATTTG ATCAACAGCC AAACCACCGA GCATATTATC 
AAACTCGTAG AGCAGCACGG CAGTGATGTG TGGTGGACCC TCCCCGCTGA GAAGCTTCTC 
CCGGAGGAAG TTGTAGCTCA GGCTGGTGGC CCCGGTGCTT TGGAGTACGT GCCAGGGCAG 
GACATTTTGG ACATCTGGTT TGACAGTGGA ACCTCGTGGT CTTGTGTTCT TCAAGGCACT 
GAGCAGAGGG CAGACTTGTA CCTGGAGGGG AAGGACCAGC TCGGGGGCTG GTTCCAGTCT 
TCGTTACTGA CAAGTGTGGC CACAAGGTCA AAAGCACCTT TCAGGACAGT GATGGTTCAT 
GGATTTACAC TCGGAGAAAA AGGAGAAAAG ATGTCCAAGT CCCTCGGGAA CGTCATTGAC 
CCTGACACTA TCATCAGTGG AGGAAAAGAC CACAGCAAAG AGCCTCCCTA CGGAGCTGAC 
ATCCTGCGCT GGTGGATAGC GGAATCCAAC GTCTTCACGG AGGTGACCAT TGGCCCCTCT 
GTACTCAGTG CTGCCAGAGA TGACATCAGC AAGCTTAGAA ACACCCTCCG CTTTCTCTTG 
GGAAATTTGG CCGGTTTTAA CCCAGAGACG GCTTCTGTTC CTGTGAAGAA TATGTACATC 
ATAGACCAGT ATATGCTGCA CCTAATTCAG GATTTTGCCA CAAAGGTTAC TGACTCATAC 
AAACAGTATG ACTTTGGAAA GGTTGTCAGG CTGTTGAAAG CCTTCTACAC CAGAGAACTG 
TCCAGCTTTT ACTTCAGCAT AGTCAAGGAT AGGCTCTACT GTGAAAGCGA GACGGATCCC 
AAGCGGCGCT CCTGTCAGAC AGCATTGGCG GAAATTCTGG ATGTGTTGGT CCGTTCTTTT 
GCGCCCGTTC TTCCTCACTT GGCAGAAGAG GTGTTCCAGC ACATCCCCTA CGTTAAAGAA 
CCCAAGAGTG TTTTTCGCAC TGGCTGGATT AGTACAAGCT CTGTTTGGAA GAAGCCAGGA 
TTGGAGGAGG CAGTGGAGAG TGCCTGTGCT ATGCGGGATT CCTTCCTTGG CAGCATCCCT 
GGAAAAAATG CTGCCGAGTA TGACGTCATC ATAGTGATCG AGCCCGGGCT GCTGTTTGAG 
ATCATGGAGA TGCTCCAGGC TGAGGAGACC TCTAGCACAT CTCAGCTGAA TGAGTTGATG 
ATGGCCTCCC AGACAACACT GTTGGCTCAG GAGCCACGGG AAAGAAGTGC AGATGCCATT 
GAGCTCACTG GGACATTCGT CATCAATCTA GAAGGTGGTG ACATTCGTGA GGAATCTTCA 
TATAAAGTCA TTGTCGTACC AACTGCCAGA GAGAAGTGCC CGCGATGTTG GAAGCATACC 
GCTGAGACTG CAGATGCTCT CTGTCCTCGG TGTGCAGAGG TCATTGGTGC AAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Iars2 ( XM_017599008.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454497.1 Rattus norvegicus isoleucyl-tRNA synthetase 2, mitochondrial (Iars2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa58177
Clone ID Related Accession (Same CDS sequence)	XM_017599008.1
Accession Version	XM_017599008.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	LOW QUALITY PROTEIN: isoleucine--tRNA ligase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 4 indels ##RefSeq-Attributes-END##

ATGCTTTGGG GGCTGCACCC TCACGGACCC GGAACGGCGG CGCTCACCGC GGCTGGGAGC 
TTATGGGGGC CAGCCCGTTT TCCCAGCCGC CTGGGGTGTC TGGGGATGAC GAGGAGGCTC 
GTGGTACGCT CCGTAGCCGG AGCGGACAGC CCCCAAAGCA GCCCGAAAAG CGGCAGATAC 
CGCGACACTG TGCTGCTGCC GCAGACCAAC TTCCCGATGA AGCTGCTGGG CCACCAGCAG 
TCCGACATGG AGCTGGAGAT CCAGCAGAAA TGTGGCTTTT CAGAGCTTTA TTCCTGGCAA 
AGAGAAAGAA AAGTAAAGAC GGAATTTTGC CTTCACGATG GCCCTCCCTA TGCAAATGGT 
GACCCTCATG TTGGGCATGC CTTAAACAAG ATTTTGAAAG ACATTGTCAA TCGATTCCAT 
ATGATGAGAG GTTCCAAGGT ACACTTTGTG CCTGGCTGGG ATTGCCATGG CTTGCCCATT 
GAAACAAAAG TGTTATCAGA GCTCGGGGTA AACACCCAGA CTCTTTCAGC CATGGAGATC 
AGGGAGAAAG CCAGGTCGTT TGCTCAAGCA GCCATTGAAA AGCAGAAATC GGCGTTTGCC 
CGCTGGGGAG TGATGGCGGA TTGGAGTAAC TGCTATTACA CATTCGATCC AAAGTATGAA 
GCCAAGCAGC TGAGAGTATT TCAGCAAATG TACGAGAAGG GCTTGGTTTA TCGATCTTAC 
AAACCAGTGT ATTGGTCTCC CTCATCGAGG ACGGCGCTGG CTGAAGCAGA GCTTGAGTAC 
AACCCTGAGC ATGTTAGTCG GTCGGTGTAT GTGAGATTCC CTCTCCTGAA GCCTCCACCT 
ACGCTGGAAT CTCTCACAGG TGCCTCATCC CCTGTTAGCG CTTTAGTCTG GACCACCCAG 
CCTTGGACAG TTCCTGCCAA TCAGGCTATA TGCTACATGC CGGAGGCCAA GTACGCTGTT 
GTGAAATGTT CTGCTTCTGG AAACTTCTAC ATCCTGGCTG AAGATAAAAT ATCACCTGTT 
GCTTCTGCCT TGGAAACAAC ATTTGATGTT ACTGCAACGT TTTCAGGTGT GGATCTGGAA 
GGTGGCACTT GTAGTCACCC TTTAATTCCC GATAAAGTGT CTCCTCTTTT ACCTGCCACT 
CACGTGACTA TGGCCAAAGG AACAGGGCTG GTCCACACAG CGCCAGCTCA CGGTATGGAA 
GATTACAGCG TAGCATCGCA GCACAGCCTC CCTATGGACT GTTTAGTGGA TGAAGAAGGA 
ATGTTCACCA GTGCTGCCGG CCCTGAACTT CAGAACAAGG CTGTGCTTAC GGAGGGGACC 
GATGTGGTTA TAAAGATGCT TCAGGCTACC AAGAATGTGT TGAAAGAAGA GAGCATTGTG 
CACAGCTATC CTTGTGACTG GAGAACTAAG AAACCCGTGC TGATTCGGGC CAGCAAGCAG 
TGGTTTGTAA ATATCATGGA TATCAAGGCT CACAGAAAAG AGTCGTTAAA GACAGTGAAG 
TTCATTCCCG GAGCAGCACT GAATAGCATG ATGGACATGC TGGACAGACG GCCGTACTGG 
TGCATATCAA GGCAGAGAGT CTGGGGTGTT CCAATCCCTG TCTTTCATCA TAAGACAAAA 
GATGAATATT TGATCAACAG CCAAACCACC GAGCATATTA TCAAACTCGT AGAGCAGCAC 
GGCAGTGATG TGTGGTGGAC CCTCCCCGCT GAGAAGCTTC TCCCGGAGGA AGTTGTAGCT 
CAGGCTGGTG GCCCCGGTGC TTTGGAGTAC GTGCCAGGGC AGGACATTTT GGACATCTGG 
TTTGACAGTG GAACCTCGTG GTCTTGTGTT CTTCAAGGCA CTGAGCAGAG GGCAGACTTG 
TACCTGGAGG GGAAGGACCA GCTCGGGGGC TGGTTCCAGT CTTCGTTACT GACAAGTGTG 
GCCACAAGGT CAAAAGCACC TTTCAGGACA GTGATGGTTC ATGGATTTAC ACTCGGAGAA 
AAAGGAGAAA AGATGTCCAA GTCCCTCGGG AACGTCATTG ACCCTGACAC TATCATCAGT 
GGAGGAAAAG ACCACAGCAA AGAGCCTCCC TACGGAGCTG ACATCCTGCG CTGGTGGATA 
GCGGAATCCA ACGTCTTCAC GGAGGTGACC ATTGGCCCCT CTGTACTCAG TGCTGCCAGA 
GATGACATCA GCAAGCTTAG AAACACCCTC CGCTTTCTCT TGGGAAATTT GGCCGGTTTT 
AACCCAGAGA CGGCTTCTGT TCCTGTGAAG AATATGTACA TCATAGACCA GTATATGCTG 
CACCTAATTC AGGATTTTGC CACAAAGGTT ACTGACTCAT ACAAACAGTA TGACTTTGGA 
AAGGTTGTCA GGCTGTTGAA AGCCTTCTAC ACCAGAGAAC TGTCCAGCTT TTACTTCAGC 
ATAGTCAAGG ATAGGCTCTA CTGTGAAAGC GAGACGGATC CCAAGCGGCG CTCCTGTCAG 
ACAGCATTGG CGGAAATTCT GGATGTGTTG GTCCGTTCTT TTGCGCCCGT TCTTCCTCAC 
TTGGCAGAAG AGGTGTTCCA GCACATCCCC TACGTTAAAG AACCCAAGAG TGTTTTTCGC 
ACTGGCTGGA TTAGTACAAG CTCTGTTTGG AAGAAGCCAG GATTGGAGGA GGCAGTGGAG 
AGTGCCTGTG CTATGCGGGA TTCCTTCCTT GGCAGCATCC CTGGAAAAAA TGCTGCCGAG 
TATGACGTCA TCATAGTGAT CGAGCCCGGG CTGCTGTTTG AGATCATGGA GATGCTCCAG 
GCTGAGGAGA CCTCTAGCAC ATCTCAGCTG AATGAGTTGA TGATGGCCTC CCAGACAACA 
CTGTTGGCTC AGGAGCCACG GGAAAGAAGT GCAGATGCCA TTGAGCTCAC TGGGACATTC 
GTCATCAATC TAGAAGGTGG TGACATTCGT GAGGAATCTT CATATAAAGT CATTGTCGTA 
CCAACTGCCA GAGAGAAGTG CCCGCGATGT TGGAAGCATA CCGCTGAGAC TGCAGATGCT 
CTCTGTCCTC GGTGTGCAGA GGTCATTGGT GCAAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454497.1
CDS	114..3152
Translation	MLWGLHPHGPGTAALTAAGSLWGPARFPSRLGCLGMTRRLVVRSVAGADSPQSSPKSGRYRDTVLLPQTNFPMKLLGHQQSDMELEIQQKCGFSELYSWQRERKVKTEFCLHDGPPYANGDPHVGHALNKILKDIVNRFHMMRGSKVHFVPGWDCHGLPIETKVLSELGVNTQTLSAMEIREKARSFAQAAIEKQKSAFARWGVMADWSNCYYTFDPKYEAKQLRVFQQMYEKGLVYRSYKPVYWSPSSRTALAEAELEYNPEHVSRSVYVRFPLLKPPPTLESLTGASSPVSALVWTTQPWTVPANQAICYMPEAKYAVVKCSASGNFYILAEDKISPVASALETTFDVTATFSGVDLEGGTCSHPLIPDKVSPLLPATHVTMAKGTGLVHTAPAHGMEDYSVASQHSLPMDCLVDEEGMFTSAAGPELQNKAVLTEGTDVVIKMLQATKNVLKEESIVHSYPCDWRTKKPVLIRASKQWFVNIMDIKAHRKESLKTVKFIPGAALNSMMDMLDRRPYWCISRQRVWGVPIPVFHHKTKDEYLINSQTTEHIIKLVEQHGSDVWWTLPAEKLLPEEVVAQAGGPGALEYVPGQDILDIWFDSGTSWSCVLQGTEQRADLYLEGKDQLGGWFQSSLLTSVATRSKAPFRTVMVHGFTLGEKGEKMSKSLGNVIDPDTIISGGKDHSKEPPYGADILRWWIAESNVFTEVTIGPSVLSAARDDISKLRNTLRFLLGNLAGFNPETASVPVKNMYIIDQYMLHLIQDFATKVTDSYKQYDFGKVVRLLKAFYTRELSSFYFSIVKDRLYCESETDPKRRSCQTALAEILDVLVRSFAPVLPHLAEEVFQHIPYVKEPKSVFRTGWISTSSVWKKPGLEEAVESACAMRDSFLGSIPGKNAAEYDVIIVIEPGLLFEIMEMLQAEETSSTSQLNELMMASQTTLLAQEPRERSADAIELTGTFVINLEGGDIREESSYKVIVVPTAREKCPRCWKHTAETADALCPRCAEVIGAK

Target ORF information:

RefSeq Version	XM_017599008.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus isoleucyl-tRNA synthetase 2, mitochondrial (Iars2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599008.1

ORF Insert Sequence:

ATGCTTTGGG GGCTGCACCC TCACGGACCC GGAACGGCGG CGCTCACCGC GGCTGGGAGC 
TTATGGGGGC CAGCCCGTTT TCCCAGCCGC CTGGGGTGTC TGGGGATGAC GAGGAGGCTC 
GTGGTACGCT CCGTAGCCGG AGCGGACAGC CCCCAAAGCA GCCCGAAAAG CGGCAGATAC 
CGCGACACTG TGCTGCTGCC GCAGACCAAC TTCCCGATGA AGCTGCTGGG CCACCAGCAG 
TCCGACATGG AGCTGGAGAT CCAGCAGAAA TGTGGCTTTT CAGAGCTTTA TTCCTGGCAA 
AGAGAAAGAA AAGTAAAGAC GGAATTTTGC CTTCACGATG GCCCTCCCTA TGCAAATGGT 
GACCCTCATG TTGGGCATGC CTTAAACAAG ATTTTGAAAG ACATTGTCAA TCGATTCCAT 
ATGATGAGAG GTTCCAAGGT ACACTTTGTG CCTGGCTGGG ATTGCCATGG CTTGCCCATT 
GAAACAAAAG TGTTATCAGA GCTCGGGGTA AACACCCAGA CTCTTTCAGC CATGGAGATC 
AGGGAGAAAG CCAGGTCGTT TGCTCAAGCA GCCATTGAAA AGCAGAAATC GGCGTTTGCC 
CGCTGGGGAG TGATGGCGGA TTGGAGTAAC TGCTATTACA CATTCGATCC AAAGTATGAA 
GCCAAGCAGC TGAGAGTATT TCAGCAAATG TACGAGAAGG GCTTGGTTTA TCGATCTTAC 
AAACCAGTGT ATTGGTCTCC CTCATCGAGG ACGGCGCTGG CTGAAGCAGA GCTTGAGTAC 
AACCCTGAGC ATGTTAGTCG GTCGGTGTAT GTGAGATTCC CTCTCCTGAA GCCTCCACCT 
ACGCTGGAAT CTCTCACAGG TGCCTCATCC CCTGTTAGCG CTTTAGTCTG GACCACCCAG 
CCTTGGACAG TTCCTGCCAA TCAGGCTATA TGCTACATGC CGGAGGCCAA GTACGCTGTT 
GTGAAATGTT CTGCTTCTGG AAACTTCTAC ATCCTGGCTG AAGATAAAAT ATCACCTGTT 
GCTTCTGCCT TGGAAACAAC ATTTGATGTT ACTGCAACGT TTTCAGGTGT GGATCTGGAA 
GGTGGCACTT GTAGTCACCC TTTAATTCCC GATAAAGTGT CTCCTCTTTT ACCTGCCACT 
CACGTGACTA TGGCCAAAGG AACAGGGCTG GTCCACACAG CGCCAGCTCA CGGTATGGAA 
GATTACAGCG TAGCATCGCA GCACAGCCTC CCTATGGACT GTTTAGTGGA TGAAGAAGGA 
ATGTTCACCA GTGCTGCCGG CCCTGAACTT CAGAACAAGG CTGTGCTTAC GGAGGGGACC 
GATGTGGTTA TAAAGATGCT TCAGGCTACC AAGAATGTGT TGAAAGAAGA GAGCATTGTG 
CACAGCTATC CTTGTGACTG GAGAACTAAG AAACCCGTGC TGATTCGGGC CAGCAAGCAG 
TGGTTTGTAA ATATCATGGA TATCAAGGCT CACAGAAAAG AGTCGTTAAA GACAGTGAAG 
TTCATTCCCG GAGCAGCACT GAATAGCATG ATGGACATGC TGGACAGACG GCCGTACTGG 
TGCATATCAA GGCAGAGAGT CTGGGGTGTT CCAATCCCTG TCTTTCATCA TAAGACAAAA 
GATGAATATT TGATCAACAG CCAAACCACC GAGCATATTA TCAAACTCGT AGAGCAGCAC 
GGCAGTGATG TGTGGTGGAC CCTCCCCGCT GAGAAGCTTC TCCCGGAGGA AGTTGTAGCT 
CAGGCTGGTG GCCCCGGTGC TTTGGAGTAC GTGCCAGGGC AGGACATTTT GGACATCTGG 
TTTGACAGTG GAACCTCGTG GTCTTGTGTT CTTCAAGGCA CTGAGCAGAG GGCAGACTTG 
TACCTGGAGG GGAAGGACCA GCTCGGGGGC TGGTTCCAGT CTTCGTTACT GACAAGTGTG 
GCCACAAGGT CAAAAGCACC TTTCAGGACA GTGATGGTTC ATGGATTTAC ACTCGGAGAA 
AAAGGAGAAA AGATGTCCAA GTCCCTCGGG AACGTCATTG ACCCTGACAC TATCATCAGT 
GGAGGAAAAG ACCACAGCAA AGAGCCTCCC TACGGAGCTG ACATCCTGCG CTGGTGGATA 
GCGGAATCCA ACGTCTTCAC GGAGGTGACC ATTGGCCCCT CTGTACTCAG TGCTGCCAGA 
GATGACATCA GCAAGCTTAG AAACACCCTC CGCTTTCTCT TGGGAAATTT GGCCGGTTTT 
AACCCAGAGA CGGCTTCTGT TCCTGTGAAG AATATGTACA TCATAGACCA GTATATGCTG 
CACCTAATTC AGGATTTTGC CACAAAGGTT ACTGACTCAT ACAAACAGTA TGACTTTGGA 
AAGGTTGTCA GGCTGTTGAA AGCCTTCTAC ACCAGAGAAC TGTCCAGCTT TTACTTCAGC 
ATAGTCAAGG ATAGGCTCTA CTGTGAAAGC GAGACGGATC CCAAGCGGCG CTCCTGTCAG 
ACAGCATTGG CGGAAATTCT GGATGTGTTG GTCCGTTCTT TTGCGCCCGT TCTTCCTCAC 
TTGGCAGAAG AGGTGTTCCA GCACATCCCC TACGTTAAAG AACCCAAGAG TGTTTTTCGC 
ACTGGCTGGA TTAGTACAAG CTCTGTTTGG AAGAAGCCAG GATTGGAGGA GGCAGTGGAG 
AGTGCCTGTG CTATGCGGGA TTCCTTCCTT GGCAGCATCC CTGGAAAAAA TGCTGCCGAG 
TATGACGTCA TCATAGTGAT CGAGCCCGGG CTGCTGTTTG AGATCATGGA GATGCTCCAG 
GCTGAGGAGA CCTCTAGCAC ATCTCAGCTG AATGAGTTGA TGATGGCCTC CCAGACAACA 
CTGTTGGCTC AGGAGCCACG GGAAAGAAGT GCAGATGCCA TTGAGCTCAC TGGGACATTC 
GTCATCAATC TAGAAGGTGG TGACATTCGT GAGGAATCTT CATATAAAGT CATTGTCGTA 
CCAACTGCCA GAGAGAAGTG CCCGCGATGT TGGAAGCATA CCGCTGAGAC TGCAGATGCT 
CTCTGTCCTC GGTGTGCAGA GGTCATTGGT GCAAAGTAG