Irak1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Irak1
Entrez Gene ID	363520
Full Name	interleukin-1 receptor-associated kinase 1
Synonyms	RGD1563841
General protein information	Preferred Names interleukin-1 receptor-associated kinase 1 Names interleukin-1 receptor-associated kinase 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq37

mRNA and Protein(s)

mRNA	Protein	Name
XM_006229598.2	XP_006229660.1	interleukin-1 receptor-associated kinase 1 isoform X1
NM_001127555.1	NP_001121027.1	interleukin-1 receptor-associated kinase 1

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	AT4G32710	NP_567903.3
Homo sapiens (human)	IRAK1	NP_001560.2
Canis lupus familiaris (dog)	IRAK1	XP_549367.2
Rattus norvegicus (Norway rat)	Irak1	NP_001121027.1
Xenopus tropicalis (tropical clawed frog)	irak1	NP_001006713.1
Danio rerio (zebrafish)	irak1	XP_005166760.1
Bos taurus (cattle)	IRAK1	NP_001035645.1
Mus musculus (house mouse)	Irak1	NP_001171446.1
Pan troglodytes (chimpanzee)	IRAK1	XP_521332.3

Gene Ontology
Bibliography

Related articles in PubMed

MicroRNA-146a Contributes to SCI Recovery via Regulating
Wei J, Wang J, Zhou Y, Yan S, Li K, Lin H
BioMed research international20164013487(2016)

MicroRNA-146a and its adapter proteins are affected by diabetes in rat's heart.
Eftekhar E, Doustaki Zaboli M, Katebi M, Soufi FG
Bratislavske lekarske listy117(3)166-70(2016)

Brain endothelial miR-146a negatively modulates T-cell adhesion through repressing multiple targets to inhibit NF-?B activation.
Wu D, Cerutti C, Lopez-Ramirez MA, Pryce G, King-Robson J, Simpson JE, van der Pol SM, Hirst MC, de Vries HE, Sharrack B, Baker D, Male DK, Michael GJ, Romero IA
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism35(3)412-23(2015 Mar)

Interleukin-1 Receptor-Associated Kinase 1/4 as a Novel Target for Inhibiting Neointimal Formation After Carotid Balloon Injury.
Bai S, Li D, Zhou Z, Cao J, Xu T, Zhang X, Wang Y, Guo J, Zhang Y
Journal of atherosclerosis and thrombosis22(12)1317-37(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

As a negative feedback element, miRNA-146a could make sure that the expression of IRAK1- and TRAF6-mediated genes was under tight control. Thus, miRNA-146a may serve as a novel therapeutic target for SCI interventions.
Title: MicroRNA-146a Contributes to SCI Recovery via Regulating

increased in the heart of diabetic rats
Title: MicroRNA-146a and its adapter proteins are affected by diabetes in rat's heart.

IRAK1/4 could serve as a potential therapeutic target to suppress neointimal formation in carotid arteries after balloon injury through the TLR4/NFkappaB signaling pathway
Title: Interleukin-1 Receptor-Associated Kinase 1/4 as a Novel Target for Inhibiting Neointimal Formation After Carotid Balloon Injury.

NSMase-2- and PP2A-dependent regulation of IRAK-1 degradation is a novel mechanism to fine tune the magnitude of IL-1beta response.
Title: Protein phosphatase 2A and neutral sphingomyelinase 2 regulate IRAK-1 protein ubiquitination and degradation in response to interleukin-1beta.

chronic elevated IL-18 levels at a supraphsiological concentration aggravated insulin resistance, enhanced vascular inflammation and remodeling, probably by increasing the level of IRAK1 and the activity of NF-kappaB
Title: IL-18 overexpression promotes vascular inflammation and remodeling in a rat model of metabolic syndrome.

The following Irak1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Irak1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01706	NM_001127555.1 Latest version!	Rattus norvegicus interleukin-1 receptor-associated kinase 1 (Irak1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$419.30 $599.00
ORa23035	XM_006229598.2 Latest version!	Rattus norvegicus interleukin-1 receptor-associated kinase 1 (Irak1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Irak1 ( NM_001127555.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001121027.1 Rattus norvegicus interleukin-1 receptor-associated kinase 1 (Irak1), mRNA.

Price & Availability↑

CloneID	ORa01706
Clone ID Related Accession (Same CDS sequence)	NM_001127555.1
Accession Version	NM_001127555.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-03
Organism	Rattus norvegicus(Norway rat)
Product	interleukin-1 receptor-associated kinase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474099.1. On or before May 23, 2008 this sequence version replaced XM_001057078.1, XM_343844.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC166780.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGGGG GCCCGGGCCC CGGGGAGCCT GTGGTTCCCG GCGCCCAGCA TTTCTTGTAC 
GAGGTGCCTC CCTGGGTTAT GTGCCGTTTC TACAAAGTGA TGGACGCCCT GGAGCCCGCC 
GACTGGTGCC AGTTCGCGGC CTTGATCGTG CGCGACCAGA CAGAGCTGCG GCTGTGTGAG 
CGCTCCGAGC AGCGCACGGC CAGTGTCCTG TGGCCCTGGA TCAACCGCAA CGCGCGTGTG 
GCTGACCTCG TTCACATCCT CACGCACCTG CAGCTGCTGC GTGCGCGGGA CATCATTACA 
GCCTGGCACC CTCCTGCCTC TGTTCTGCCC CCAAGCACCG GTGCCCCAAG GCCCAGCAGC 
ATCTCTGCAG GCTCTGAGAC TGAGGACTGG AGCCCCCGGA AATTGCAGTC CTCTGCCTCC 
ACCTTCCTCT CCCCAGCTTT TCCAGGCTCC CAGACCCATT CTGAGTCAGA GCTCCTCCAG 
GTTCCACTCT CTGATTCCCT CGGGCCACCA CTACAATCTT CAGCCCCCTC CTCCATCAAG 
CCAAGCCCAG AGAGCCCAGT GTCTGGCCTC CAAAGAGCTC GTCCCTCCCC ATTTTGCTGG 
CCCTTCTGTG AGATTTCCCA AGGCACTTGC AACTTCTCTG AAGAACTCAG GATTGGAGAG 
GGTGGTTTTG GGTGTGTGTA CCGAGCAGTA ATGAGAAATA CTACATATGC TGTGAAGAGA 
CTGAAGGAGG AGGCTGACCT AGAGTGGACT GTGGTGAAAC AGAGCTTCCT AACAGAGGTG 
GAACAGCTAT CAAGGTTTCG TCACCCAAAT ATCGTGGACT TTGCTGGCTA CTGTGCAGAG 
AGTGGCTTCT ACTGCCTTGT TTATGGCTTC CTGCCCAATG GCTCCTTAGA GGATCAGCTT 
CACCTTCAGA CCCAAGCCTG CTCCCCACTT TCCTGGCCTC AACGACTGGA CATTCTTCTG 
GGCACAGCCC GGGCTATTCA GTTTTTACAT CAGGACAGCC CCAGCCTTAT CCATGGAGAC 
ATCAAGAGTT CTAATGTGCT TCTGGATGAG AGACTGATGC CCAAGCTGGG AGACTTTGGT 
CTGGCTCGTT TCAGCCGCTT TGCAGGGGCC AACCCAAGCC AGAGCAGTAC TGTGGCCCGG 
ACTTGCACAG TTCGAGGTAC CCTCGCCTAC CTGCCTGAGG AGTACATCAA GACAGGCCGG 
CTGGCTGTGG ACACCGATAC CTTCAGCTTT GGGGTGGTAA TACTGGAGAC CCTTGCTGGT 
CAGAGGGCTG TGAGGACACA AGGTGCAAAA ACCAAATATC TGAAAGACCT GATTGAAGAT 
GAGGCTGAAG AGGTGGGAGT GACCTTAAAA AGCACCCAGC CTACTTTGTG GATGGGTGTA 
GCCACGGATG CTTGGACTGC TCCAATTGCT GCCCAGATCT ATAAGAAGCA CCTGGACTCC 
AGACCTGGGC CCTGCCCACC CCAGTTGGGC CTGGCCCTGG CTCAACTAGC TTGCTGCTGC 
ATGCACCGTC GGGCCAAGAA GAGGCCACCC ATGACCCAGG TATACAAGAG ACTAGAAGGG 
CTTCAGGCAG TACCTCCCTG GAAGCTAGAG GTTGCCGGCC ATGGCTCTCC TTCCCCACAG 
GAGAACTCCT ACATGTCCAC CACTGGCAGT GCCCAGAGTG GGGATGAACC ATGGCAGCCT 
CTGGTAGTGA CCACAAGAGC TCCAGCCCAG GCTGCCCAGC AACTCCAGAG AAGTCCCAAC 
CAGCCAGTGG AGAGTGATGA GAGTGTTCCT GGCCTCTCTG CTACCCTGCA TTCCTGGCAC 
TTGACTCCAG ATTCCCACCC AAGCCCTGTG TCCTTCAGAG AGGCTAGCTG TACACAAGGA 
GGCACTACCA GAGAATCAAG TTTGAGGAGT GGCCCAGGCT TCCAGCCTAC GACCATGGAA 
GGCTCATCGA TGGGCAGTTC ATCCCTGCTG TCATCAGAGC CACCACAGAT CATCATCAAC 
CCAGCCCGAC AGAAGATGGT ACAAAAGCTG GCCCTGTATG AAGAGGGGGT CTTGGATAGC 
CTGCAACTGC TGTCATCAGG CTTTTTCCCA GGCTTGGATT TAGAACCTGA AAAGAGAAAG 
AGACCTGAAG AAAGTGATGA ATTCCAGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121027.1
CDS	182..2314
Translation	MAGGPGPGEPVVPGAQHFLYEVPPWVMCRFYKVMDALEPADWCQFAALIVRDQTELRLCERSEQRTASVLWPWINRNARVADLVHILTHLQLLRARDIITAWHPPASVLPPSTGAPRPSSISAGSETEDWSPRKLQSSASTFLSPAFPGSQTHSESELLQVPLSDSLGPPLQSSAPSSIKPSPESPVSGLQRARPSPFCWPFCEISQGTCNFSEELRIGEGGFGCVYRAVMRNTTYAVKRLKEEADLEWTVVKQSFLTEVEQLSRFRHPNIVDFAGYCAESGFYCLVYGFLPNGSLEDQLHLQTQACSPLSWPQRLDILLGTARAIQFLHQDSPSLIHGDIKSSNVLLDERLMPKLGDFGLARFSRFAGANPSQSSTVARTCTVRGTLAYLPEEYIKTGRLAVDTDTFSFGVVILETLAGQRAVRTQGAKTKYLKDLIEDEAEEVGVTLKSTQPTLWMGVATDAWTAPIAAQIYKKHLDSRPGPCPPQLGLALAQLACCCMHRRAKKRPPMTQVYKRLEGLQAVPPWKLEVAGHGSPSPQENSYMSTTGSAQSGDEPWQPLVVTTRAPAQAAQQLQRSPNQPVESDESVPGLSATLHSWHLTPDSHPSPVSFREASCTQGGTTRESSLRSGPGFQPTTMEGSSMGSSSLLSSEPPQIIINPARQKMVQKLALYEEGVLDSLQLLSSGFFPGLDLEPEKRKRPEESDEFQS

Target ORF information:

RefSeq Version	NM_001127555.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus interleukin-1 receptor-associated kinase 1 (Irak1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127555.1

ORF Insert Sequence:

ATGGCCGGGG GCCCGGGCCC CGGGGAGCCT GTGGTTCCCG GCGCCCAGCA TTTCTTGTAC 
GAGGTGCCTC CCTGGGTTAT GTGCCGTTTC TACAAAGTGA TGGACGCCCT GGAGCCCGCC 
GACTGGTGCC AGTTCGCGGC CTTGATCGTG CGCGACCAGA CAGAGCTGCG GCTGTGTGAG 
CGCTCCGAGC AGCGCACGGC CAGTGTCCTG TGGCCCTGGA TCAACCGCAA CGCGCGTGTG 
GCTGACCTCG TTCACATCCT CACGCACCTG CAGCTGCTGC GTGCGCGGGA CATCATTACA 
GCCTGGCACC CTCCTGCCTC TGTTCTGCCC CCAAGCACCG GTGCCCCAAG GCCCAGCAGC 
ATCTCTGCAG GCTCTGAGAC TGAGGACTGG AGCCCCCGGA AATTGCAGTC CTCTGCCTCC 
ACCTTCCTCT CCCCAGCTTT TCCAGGCTCC CAGACCCATT CTGAGTCAGA GCTCCTCCAG 
GTTCCACTCT CTGATTCCCT CGGGCCACCA CTACAATCTT CAGCCCCCTC CTCCATCAAG 
CCAAGCCCAG AGAGCCCAGT GTCTGGCCTC CAAAGAGCTC GTCCCTCCCC ATTTTGCTGG 
CCCTTCTGTG AGATTTCCCA AGGCACTTGC AACTTCTCTG AAGAACTCAG GATTGGAGAG 
GGTGGTTTTG GGTGTGTGTA CCGAGCAGTA ATGAGAAATA CTACATATGC TGTGAAGAGA 
CTGAAGGAGG AGGCTGACCT AGAGTGGACT GTGGTGAAAC AGAGCTTCCT AACAGAGGTG 
GAACAGCTAT CAAGGTTTCG TCACCCAAAT ATCGTGGACT TTGCTGGCTA CTGTGCAGAG 
AGTGGCTTCT ACTGCCTTGT TTATGGCTTC CTGCCCAATG GCTCCTTAGA GGATCAGCTT 
CACCTTCAGA CCCAAGCCTG CTCCCCACTT TCCTGGCCTC AACGACTGGA CATTCTTCTG 
GGCACAGCCC GGGCTATTCA GTTTTTACAT CAGGACAGCC CCAGCCTTAT CCATGGAGAC 
ATCAAGAGTT CTAATGTGCT TCTGGATGAG AGACTGATGC CCAAGCTGGG AGACTTTGGT 
CTGGCTCGTT TCAGCCGCTT TGCAGGGGCC AACCCAAGCC AGAGCAGTAC TGTGGCCCGG 
ACTTGCACAG TTCGAGGTAC CCTCGCCTAC CTGCCTGAGG AGTACATCAA GACAGGCCGG 
CTGGCTGTGG ACACCGATAC CTTCAGCTTT GGGGTGGTAA TACTGGAGAC CCTTGCTGGT 
CAGAGGGCTG TGAGGACACA AGGTGCAAAA ACCAAATATC TGAAAGACCT GATTGAAGAT 
GAGGCTGAAG AGGTGGGAGT GACCTTAAAA AGCACCCAGC CTACTTTGTG GATGGGTGTA 
GCCACGGATG CTTGGACTGC TCCAATTGCT GCCCAGATCT ATAAGAAGCA CCTGGACTCC 
AGACCTGGGC CCTGCCCACC CCAGTTGGGC CTGGCCCTGG CTCAACTAGC TTGCTGCTGC 
ATGCACCGTC GGGCCAAGAA GAGGCCACCC ATGACCCAGG TATACAAGAG ACTAGAAGGG 
CTTCAGGCAG TACCTCCCTG GAAGCTAGAG GTTGCCGGCC ATGGCTCTCC TTCCCCACAG 
GAGAACTCCT ACATGTCCAC CACTGGCAGT GCCCAGAGTG GGGATGAACC ATGGCAGCCT 
CTGGTAGTGA CCACAAGAGC TCCAGCCCAG GCTGCCCAGC AACTCCAGAG AAGTCCCAAC 
CAGCCAGTGG AGAGTGATGA GAGTGTTCCT GGCCTCTCTG CTACCCTGCA TTCCTGGCAC 
TTGACTCCAG ATTCCCACCC AAGCCCTGTG TCCTTCAGAG AGGCTAGCTG TACACAAGGA 
GGCACTACCA GAGAATCAAG TTTGAGGAGT GGCCCAGGCT TCCAGCCTAC GACCATGGAA 
GGCTCATCGA TGGGCAGTTC ATCCCTGCTG TCATCAGAGC CACCACAGAT CATCATCAAC 
CCAGCCCGAC AGAAGATGGT ACAAAAGCTG GCCCTGTATG AAGAGGGGGT CTTGGATAGC 
CTGCAACTGC TGTCATCAGG CTTTTTCCCA GGCTTGGATT TAGAACCTGA AAAGAGAAAG 
AGACCTGAAG AAAGTGATGA ATTCCAGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Irak1 ( XM_006229598.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229660.1 Rattus norvegicus interleukin-1 receptor-associated kinase 1 (Irak1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa23035
Clone ID Related Accession (Same CDS sequence)	XM_006229598.2
Accession Version	XM_006229598.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	interleukin-1 receptor-associated kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229598.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG GCCCGGGCCC CGGGGAGCCT GTGGTTCCCG GCGCCCAGCA TTTCTTGTAC 
GAGGTGCCTC CCTGGGTTAT GTGCCGTTTC TACAAAGTGA TGGACGCCCT GGAGCCCGCC 
GACTGGTGCC AGTTCGCGGC CTTGATCGTG CGCGACCAGA CAGAGCTGCG GCTGTGTGAG 
CGCTCCGAGC AGCGCACGGC CAGTGTCCTG TGGCCCTGGA TCAACCGCAA CGCGCGTGTG 
GCTGACCTCG TTCACATCCT CACGCACCTG CAGCTGCTGC GTGCGCGGGA CATCATTACA 
GCCTGGCACC CTCCTGCCTC TGTTCTGCCC CCAAGCACCG GTGCCCCAAG GCCCAGCAGC 
ATCTCTGCAG GCTCTGAGAC TGAGGACTGG AGCCCCCGGA AATTGCAGTC CTCTGCCTCC 
ACCTTCCTCT CCCCAGCTTT TCCAGGCTCC CAGACCCATT CTGAGTCAGA GCTCCTCCAG 
GTTCCACTCT CTGATTCCCT CGGGCCACCA CTACAATCTT CAGCCCCCTC CTCCATCAAG 
CAGCCAAGCC CAGAGAGCCC AGTGTCTGGC CTCCAAAGAG CTCGTCCCTC CCCATTTTGC 
TGGCCCTTCT GTGAGATTTC CCAAGGCACT TGCAACTTCT CTGAAGAACT CAGGATTGGA 
GAGGGTGGTT TTGGGTGTGT GTACCGAGCA GTAATGAGAA ATACTACATA TGCTGTGAAG 
AGACTGAAGG AGGAGGCTGA CCTAGAGTGG ACTGTGGTGA AACAGAGCTT CCTAACAGAG 
GTGGAACAGC TATCAAGGTT TCGTCACCCA AATATCGTGG ACTTTGCTGG CTACTGTGCA 
GAGAGTGGCT TCTACTGCCT TGTTTATGGC TTCCTGCCCA ATGGCTCCTT AGAGGATCAG 
CTTCACCTTC AGACCCAAGC CTGCTCCCCA CTTTCCTGGC CTCAACGACT GGACATTCTT 
CTGGGCACAG CCCGGGCTAT TCAGTTTTTA CATCAGGACA GCCCCAGCCT TATCCATGGA 
GACATCAAGA GTTCTAATGT GCTTCTGGAT GAGAGACTGA TGCCCAAGCT GGGAGACTTT 
GGTCTGGCTC GTTTCAGCCG CTTTGCAGGG GCCAACCCAA GCCAGAGCAG TACTGTGGCC 
CGGACTTGCA CAGTTCGAGG TACCCTCGCC TACCTGCCTG AGGAGTACAT CAAGACAGGC 
CGGCTGGCTG TGGACACCGA TACCTTCAGC TTTGGGGTGG TAATACTGGA GACCCTTGCT 
GGTCAGAGGG CTGTGAGGAC ACAAGGTGCA AAAACCAAAT ATCTGAAAGA CCTGATTGAA 
GATGAGGCTG AAGAGGTGGG AGTGACCTTA AAAAGCACCC AGCCTACTTT GTGGATGGGT 
GTAGCCACGG ATGCTTGGAC TGCTCCAATT GCTGCCCAGA TCTATAAGAA GCACCTGGAC 
TCCAGACCTG GGCCCTGCCC ACCCCAGTTG GGCCTGGCCC TGGCTCAACT AGCTTGCTGC 
TGCATGCACC GTCGGGCCAA GAAGAGGCCA CCCATGACCC AGGTATACAA GAGACTAGAA 
GGGCTTCAGG CAGTACCTCC CTGGAAGCTA GAGGTTGCCG GCCATGGCTC TCCTTCCCCA 
CAGGAGAACT CCTACATGTC CACCACTGGC AGTGCCCAGA GTGGGGATGA ACCATGGCAG 
CCTCTGGTAG TGACCACAAG AGCTCCAGCC CAGGCTGCCC AGCAACTCCA GAGAAGTCCC 
AACCAGCCAG TGGAGAGTGA TGAGAGTGTT CCTGGCCTCT CTGCTACCCT GCATTCCTGG 
CACTTGACTC CAGATTCCCA CCCAAGCCCT GTGTCCTTCA GAGAGGCTAG CTGTACACAA 
GGAGGCACTA CCAGAGAATC AAGTTTGAGG AGTGGCCCAG GCTTCCAGCC TACGACCATG 
GAAGGCTCAT CGATGGGCAG TTCATCCCTG CTGTCATCAG AGCCACCACA GATCATCATC 
AACCCAGCCC GACAGAAGAT GGTACAAAAG CTGGCCCTGT ATGAAGAGGG GGTCTTGGAT 
AGCCTGCAAC TGCTGTCATC AGGCTTTTTC CCAGGCTTGG ATTTAGAACC TGAAAAGAGA 
AAGAGACCTG AAGAAAGTGA TGAATTCCAG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229660.1
CDS	162..2297
Translation	MAGGPGPGEPVVPGAQHFLYEVPPWVMCRFYKVMDALEPADWCQFAALIVRDQTELRLCERSEQRTASVLWPWINRNARVADLVHILTHLQLLRARDIITAWHPPASVLPPSTGAPRPSSISAGSETEDWSPRKLQSSASTFLSPAFPGSQTHSESELLQVPLSDSLGPPLQSSAPSSIKQPSPESPVSGLQRARPSPFCWPFCEISQGTCNFSEELRIGEGGFGCVYRAVMRNTTYAVKRLKEEADLEWTVVKQSFLTEVEQLSRFRHPNIVDFAGYCAESGFYCLVYGFLPNGSLEDQLHLQTQACSPLSWPQRLDILLGTARAIQFLHQDSPSLIHGDIKSSNVLLDERLMPKLGDFGLARFSRFAGANPSQSSTVARTCTVRGTLAYLPEEYIKTGRLAVDTDTFSFGVVILETLAGQRAVRTQGAKTKYLKDLIEDEAEEVGVTLKSTQPTLWMGVATDAWTAPIAAQIYKKHLDSRPGPCPPQLGLALAQLACCCMHRRAKKRPPMTQVYKRLEGLQAVPPWKLEVAGHGSPSPQENSYMSTTGSAQSGDEPWQPLVVTTRAPAQAAQQLQRSPNQPVESDESVPGLSATLHSWHLTPDSHPSPVSFREASCTQGGTTRESSLRSGPGFQPTTMEGSSMGSSSLLSSEPPQIIINPARQKMVQKLALYEEGVLDSLQLLSSGFFPGLDLEPEKRKRPEESDEFQS

Target ORF information:

RefSeq Version	XM_006229598.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus interleukin-1 receptor-associated kinase 1 (Irak1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229598.2

ORF Insert Sequence:

ATGGCCGGGG GCCCGGGCCC CGGGGAGCCT GTGGTTCCCG GCGCCCAGCA TTTCTTGTAC 
GAGGTGCCTC CCTGGGTTAT GTGCCGTTTC TACAAAGTGA TGGACGCCCT GGAGCCCGCC 
GACTGGTGCC AGTTCGCGGC CTTGATCGTG CGCGACCAGA CAGAGCTGCG GCTGTGTGAG 
CGCTCCGAGC AGCGCACGGC CAGTGTCCTG TGGCCCTGGA TCAACCGCAA CGCGCGTGTG 
GCTGACCTCG TTCACATCCT CACGCACCTG CAGCTGCTGC GTGCGCGGGA CATCATTACA 
GCCTGGCACC CTCCTGCCTC TGTTCTGCCC CCAAGCACCG GTGCCCCAAG GCCCAGCAGC 
ATCTCTGCAG GCTCTGAGAC TGAGGACTGG AGCCCCCGGA AATTGCAGTC CTCTGCCTCC 
ACCTTCCTCT CCCCAGCTTT TCCAGGCTCC CAGACCCATT CTGAGTCAGA GCTCCTCCAG 
GTTCCACTCT CTGATTCCCT CGGGCCACCA CTACAATCTT CAGCCCCCTC CTCCATCAAG 
CAGCCAAGCC CAGAGAGCCC AGTGTCTGGC CTCCAAAGAG CTCGTCCCTC CCCATTTTGC 
TGGCCCTTCT GTGAGATTTC CCAAGGCACT TGCAACTTCT CTGAAGAACT CAGGATTGGA 
GAGGGTGGTT TTGGGTGTGT GTACCGAGCA GTAATGAGAA ATACTACATA TGCTGTGAAG 
AGACTGAAGG AGGAGGCTGA CCTAGAGTGG ACTGTGGTGA AACAGAGCTT CCTAACAGAG 
GTGGAACAGC TATCAAGGTT TCGTCACCCA AATATCGTGG ACTTTGCTGG CTACTGTGCA 
GAGAGTGGCT TCTACTGCCT TGTTTATGGC TTCCTGCCCA ATGGCTCCTT AGAGGATCAG 
CTTCACCTTC AGACCCAAGC CTGCTCCCCA CTTTCCTGGC CTCAACGACT GGACATTCTT 
CTGGGCACAG CCCGGGCTAT TCAGTTTTTA CATCAGGACA GCCCCAGCCT TATCCATGGA 
GACATCAAGA GTTCTAATGT GCTTCTGGAT GAGAGACTGA TGCCCAAGCT GGGAGACTTT 
GGTCTGGCTC GTTTCAGCCG CTTTGCAGGG GCCAACCCAA GCCAGAGCAG TACTGTGGCC 
CGGACTTGCA CAGTTCGAGG TACCCTCGCC TACCTGCCTG AGGAGTACAT CAAGACAGGC 
CGGCTGGCTG TGGACACCGA TACCTTCAGC TTTGGGGTGG TAATACTGGA GACCCTTGCT 
GGTCAGAGGG CTGTGAGGAC ACAAGGTGCA AAAACCAAAT ATCTGAAAGA CCTGATTGAA 
GATGAGGCTG AAGAGGTGGG AGTGACCTTA AAAAGCACCC AGCCTACTTT GTGGATGGGT 
GTAGCCACGG ATGCTTGGAC TGCTCCAATT GCTGCCCAGA TCTATAAGAA GCACCTGGAC 
TCCAGACCTG GGCCCTGCCC ACCCCAGTTG GGCCTGGCCC TGGCTCAACT AGCTTGCTGC 
TGCATGCACC GTCGGGCCAA GAAGAGGCCA CCCATGACCC AGGTATACAA GAGACTAGAA 
GGGCTTCAGG CAGTACCTCC CTGGAAGCTA GAGGTTGCCG GCCATGGCTC TCCTTCCCCA 
CAGGAGAACT CCTACATGTC CACCACTGGC AGTGCCCAGA GTGGGGATGA ACCATGGCAG 
CCTCTGGTAG TGACCACAAG AGCTCCAGCC CAGGCTGCCC AGCAACTCCA GAGAAGTCCC 
AACCAGCCAG TGGAGAGTGA TGAGAGTGTT CCTGGCCTCT CTGCTACCCT GCATTCCTGG 
CACTTGACTC CAGATTCCCA CCCAAGCCCT GTGTCCTTCA GAGAGGCTAG CTGTACACAA 
GGAGGCACTA CCAGAGAATC AAGTTTGAGG AGTGGCCCAG GCTTCCAGCC TACGACCATG 
GAAGGCTCAT CGATGGGCAG TTCATCCCTG CTGTCATCAG AGCCACCACA GATCATCATC 
AACCCAGCCC GACAGAAGAT GGTACAAAAG CTGGCCCTGT ATGAAGAGGG GGTCTTGGAT 
AGCCTGCAAC TGCTGTCATC AGGCTTTTTC CCAGGCTTGG ATTTAGAACC TGAAAAGAGA 
AAGAGACCTG AAGAAAGTGA TGAATTCCAG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MicroRNA-146a Contributes to SCI Recovery via Regulating
BioMed research international20164013487(2016)
Wei J,Wang J,Zhou Y,Yan S,Li K,Lin H

MicroRNA-146a and its adapter proteins are affected by diabetes in rat's heart.
Bratislavske lekarske listy117(3)166-70(2016)
Eftekhar E,Doustaki Zaboli M,Katebi M,Soufi FG

Brain endothelial miR-146a negatively modulates T-cell adhesion through repressing multiple targets to inhibit NF-?B activation.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism35(3)412-23(2015 Mar)
Wu D,Cerutti C,Lopez-Ramirez MA,Pryce G,King-Robson J,Simpson JE,van der Pol SM,Hirst MC,de Vries HE,Sharrack B,Baker D,Male DK,Michael GJ,Romero IA

Interleukin-1 Receptor-Associated Kinase 1/4 as a Novel Target for Inhibiting Neointimal Formation After Carotid Balloon Injury.
Journal of atherosclerosis and thrombosis22(12)1317-37(2015)
Bai S,Li D,Zhou Z,Cao J,Xu T,Zhang X,Wang Y,Guo J,Zhang Y